Rw5G001750

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0002602
Physical Location & Seq
Forward (+)
1527406 .. 1532029
4624 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G001750.1

Sequence Viewer

Length: 1101 bp
ATGGTTCCTTTACCTACTGAAGCTTCAATCAAATGGGATTCTCAGCAGCCAGACCTCTACTCTCCTTCTTCTTCTTCTTCTTCTTCTTCATTATCCCGCGCGAGATCTCGAATTTCTCGCCACAGCGTCAGCTTCGCCGGCCCCGCCAAGATCGCCTTCCTATTTCTCGCGCGACGTGACCTCCCGCTCGATTTTCTTTGGGAGAGCTTCTTCGAGAATGCCGGTGGCGCTTTGAATTTTTCGATTTATATTCACTCGGCGCCTGGTTTTGTGTTCGATGAGTCCACGACGAGGTCTCGCTTCTTTCACGGCCGTCAGTTGCCGAATAGCATACAGGTGGGATGGGGAGAATCGAGCATGATTGAAGCGGAGAGGTTGTTGTTTGCAACAGCTCTTGAGGATCCGGCAAATCAAAGATTTGTTCTCCTCTCTGACAGTTGCGTTCCTTTGTACAACTTCAGCTTCATATACAATTACCTGATGGCTTCTCGTAGGAGTATTGTGGACAGCTTCCTGGATGTGAAGGAGGGCCGCTACAACCCAAAAATGTCACCCATAATACCAAAAGCCAAATGGCGGAAAGGATCACAGTGGATTGCTTTGATTCGGAGACACGCTGAAGTCCTTGTGGATGATGAAGTTATACTTCCAGTTTTCAAAAAGTTTTGCAGACGTCGTCCACCTGTTGATATCAGAAAGGGGAAGCTGAATCTTAAACTTCAGAAACAGCACAACTGTATCCCAGACGAACACTATGTGCAGACACTGCTTGCGATGAGTGAGCTTGACGGCGAACTTGAGCGAAGGACATTAACCTATACATTGTGGAGCAACTCTCCAAATAAAACAGAGAGTAAAGGCTGGCATCCTATGACTTTCAACCATGCCAATGCGGCCCCTCAAAAGATTAGGGACATAAAGGGGATCAACCATGTATACTATGAGACAGAATTCCGAACAGAATGGTGTCGTATAAATTCTACTGTTGTTCCCTGTTTTTTATTCGCAAGGAAGTTCTCCAAGGGTGCTGCCATGCGCCTTCTGAGCGAGGGAGTTGTTGGTCACTTTGACACAGCTGCATTGTTAAAACCATCACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

366

Amino Acids

41.83

Weight (kDa)

9.49

Isoelectric Point (pI)

57.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 51 - 306 1.5e-68 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 676
AccB1I GGYRCC 1 cut(s) 259
AccBSI CCGCTC 1 cut(s) 187
AccI GTMKAC 1 cut(s) 936
AccII CGCG 4 cut(s) 99, 101, 170, 172
AciI CCGC 7 cut(s) 97, 144, 185, 368, 532, 577, 893
AclWI GGATC 4 cut(s) 395, 408, 592, 932
AcoI YGGCCR 1 cut(s) 310
AcsI RAATTY 4 cut(s) 111, 235, 950, 976
AcuI CTGAAG 4 cut(s) 39, 442, 639, 704
AcyI GRCGYC 2 cut(s) 260, 673
AdeI CACNNNGTG 1 cut(s) 757
AfaI GTAC 1 cut(s) 452
AfiI CCNNNNNNNGG 2 cut(s) 291, 576
AgsI TTSAA 5 cut(s) 27, 235, 365, 658, 880
AjiI CACGTC 1 cut(s) 176
AjnI CCWGG 2 cut(s) 262, 513
AjuI GAANNNNNNNTTGG 2 cut(s) 140, 172
AluBI AGCT 9 cut(s) 23, 132, 207, 392, 462, 510, 706, 784, 1076
AluI AGCT 9 cut(s) 23, 132, 207, 392, 462, 510, 706, 784, 1076
Alw26I GTCTC 3 cut(s) 300, 604, 938
AlwI GGATC 4 cut(s) 395, 408, 592, 932
AlwNI CAGNNNCTG 1 cut(s) 766
AoxI GGCC 4 cut(s) 139, 310, 529, 894
ApeKI GCWGC 3 cut(s) 46, 1028, 1076
ApoI RAATTY 4 cut(s) 111, 235, 950, 976
AspLEI GCGC 5 cut(s) 101, 172, 230, 262, 1038
AspS9I GGNCC 3 cut(s) 140, 529, 895
AsuHPI GGTGA 2 cut(s) 543, 1086
BaeI ACNNNNGTAYC 2 cut(s) 721, 754
BamHI GGATCC 1 cut(s) 400
BanI GGYRCC 1 cut(s) 259
BbvI GCAGC 3 cut(s) 58, 1015, 1063
BccI CCATC 3 cut(s) 336, 475, 1099
BceAI ACGGC 3 cut(s) 297, 325, 805
BciT130I CCWGG 2 cut(s) 264, 515
BciVI GTATCC 1 cut(s) 749
BcoDI GTCTC 3 cut(s) 300, 604, 938
BfoI RGCGCY 2 cut(s) 231, 263
BfuI GTATCC 1 cut(s) 749
BglI GCCNNNNNGGC 1 cut(s) 893
BglII AGATCT 1 cut(s) 104
BisI GCNGC 5 cut(s) 47, 532, 894, 1029, 1077
BlsI GCNGC 5 cut(s) 48, 533, 895, 1030, 1078
Bme1390I CCNGG 2 cut(s) 264, 515
BmgBI CACGTC 1 cut(s) 176
BmgT120I GGNCC 3 cut(s) 140, 529, 895
BmiI GGNNCC 5 cut(s) 6, 142, 261, 402, 897
BmrFI CCNGG 2 cut(s) 264, 515
BmsI GCATC 1 cut(s) 874
BplI GAGNNNNNCTC 2 cut(s) 820, 852
BpuEI CTTGAG 2 cut(s) 416, 818
BsaHI GRCGYC 2 cut(s) 260, 673
BsaI GGTCTC 1 cut(s) 300
BsaJI CCNNGG 1 cut(s) 1020
BsaXI ACNNNNNCTCC 4 cut(s) 165, 195, 362, 392
Bsc4I CCNNNNNNNGG 2 cut(s) 291, 576
Bse118I RCCGGY 2 cut(s) 137, 221
Bse1I ACTGG 1 cut(s) 650
BseBI CCWGG 2 cut(s) 264, 515
BseDI CCNNGG 1 cut(s) 1020
BseGI GGATG 4 cut(s) 347, 523, 637, 865
BseLI CCNNNNNNNGG 2 cut(s) 291, 576
BseMII CTCAG 2 cut(s) 56, 1034
BseNI ACTGG 1 cut(s) 650
BseRI GAGGAG 1 cut(s) 416
BseX3I CGGCCG 1 cut(s) 310
BseXI GCAGC 3 cut(s) 58, 1015, 1063
BsgI GTGCAG 1 cut(s) 779
Bsh1236I CGCG 4 cut(s) 99, 101, 170, 172
Bsh1285I CGRYCG 1 cut(s) 313
BshFI GGCC 4 cut(s) 141, 312, 531, 896
BshNI GGYRCC 1 cut(s) 259
BsiEI CGRYCG 1 cut(s) 313
BsiSI CCGG 3 cut(s) 138, 222, 404
BslFI GGGAC 1 cut(s) 926
BslI CCNNNNNNNGG 2 cut(s) 291, 576
BsmAI GTCTC 3 cut(s) 300, 604, 938
BsmFI GGGAC 1 cut(s) 926
BsmI GAATGC 1 cut(s) 223
BsnI GGCC 4 cut(s) 141, 312, 531, 896
Bso31I GGTCTC 1 cut(s) 300
Bsp1407I TGTACA 1 cut(s) 450
Bsp143I GATC 5 cut(s) 104, 150, 400, 584, 924
BspACI CCGC 7 cut(s) 97, 144, 185, 368, 532, 577, 893
BspANI GGCC 4 cut(s) 141, 312, 531, 896
BspCNI CTCAG 2 cut(s) 55, 1035
BspFNI CGCG 4 cut(s) 99, 101, 170, 172
BspLI GGNNCC 5 cut(s) 6, 142, 261, 402, 897
BspPI GGATC 4 cut(s) 395, 408, 592, 932
BspT107I GGYRCC 1 cut(s) 259
BspTNI GGTCTC 1 cut(s) 300
BsrBI CCGCTC 1 cut(s) 187
BsrFI RCCGGY 2 cut(s) 137, 221
BsrGI TGTACA 1 cut(s) 450
BsrI ACTGG 1 cut(s) 650
BssAI RCCGGY 2 cut(s) 137, 221
BssECI CCNNGG 1 cut(s) 1020
BssMI GATC 5 cut(s) 104, 150, 400, 584, 924
BssNAI GTATAC 1 cut(s) 937
BssNI GRCGYC 2 cut(s) 260, 673
BssT1I CCWWGG 1 cut(s) 1020
Bst1107I GTATAC 1 cut(s) 937
Bst2UI CCWGG 2 cut(s) 264, 515
Bst4CI ACNGT 4 cut(s) 437, 591, 737, 985
BstACI GRCGYC 2 cut(s) 260, 673
BstAPI GCANNNNNTGC 1 cut(s) 766
BstAUI TGTACA 1 cut(s) 450
BstC8I GCNNGC 3 cut(s) 139, 771, 863
BstDEI CTNAG 2 cut(s) 42, 1043
BstF5I GGATG 4 cut(s) 347, 523, 637, 865
BstFNI CGCG 4 cut(s) 99, 101, 170, 172
BstH2I RGCGCY 2 cut(s) 231, 263
BstHHI GCGC 5 cut(s) 101, 172, 230, 262, 1038
BstKTI GATC 5 cut(s) 107, 153, 403, 587, 927
BstMAI GTCTC 3 cut(s) 300, 604, 938
BstMBI GATC 5 cut(s) 104, 150, 400, 584, 924
BstMCI CGRYCG 1 cut(s) 313
BstMWI GCNNNNNNNGC 7 cut(s) 138, 143, 152, 227, 766, 893, 1044
BstNI CCWGG 2 cut(s) 264, 515
BstSCI CCNGG 2 cut(s) 262, 513
BstUI CGCG 4 cut(s) 99, 101, 170, 172
BstV1I GCAGC 3 cut(s) 58, 1015, 1063
BstX2I RGATCY 2 cut(s) 104, 400
BstYI RGATCY 2 cut(s) 104, 400
BstZ17I GTATAC 1 cut(s) 937
BstZI CGGCCG 1 cut(s) 310
BsuI GTATCC 1 cut(s) 749
BsuRI GGCC 4 cut(s) 141, 312, 531, 896
BtgZI GCGATG 1 cut(s) 788
BtrI CACGTC 1 cut(s) 176
BtsCI GGATG 4 cut(s) 347, 523, 637, 865
BtsI GCAGTG 1 cut(s) 764
BtsIMutI CAGTG 2 cut(s) 596, 764
Cac8I GCNNGC 3 cut(s) 139, 771, 863
CaiI CAGNNNCTG 1 cut(s) 766
CfoI GCGC 5 cut(s) 101, 172, 230, 262, 1038
Cfr10I RCCGGY 2 cut(s) 137, 221
Cfr13I GGNCC 3 cut(s) 140, 529, 895
CseI GACGC 1 cut(s) 115
Csp6I GTAC 1 cut(s) 451
CviAII CATG 5 cut(s) 358, 884, 932, 1033, 1098
CviQI GTAC 1 cut(s) 451
DdeI CTNAG 2 cut(s) 42, 1043
DinI GGCGCC 1 cut(s) 261
DpnI GATC 5 cut(s) 106, 152, 402, 586, 926
DpnII GATC 5 cut(s) 104, 150, 400, 584, 924
DraIII CACNNNGTG 1 cut(s) 757
EaeI YGGCCR 1 cut(s) 310
EagI CGGCCG 1 cut(s) 310
EciI GGCGGA 1 cut(s) 592
EclXI CGGCCG 1 cut(s) 310
Eco130I CCWWGG 1 cut(s) 1020
Eco31I GGTCTC 1 cut(s) 300
Eco32I GATATC 1 cut(s) 691
Eco52I CGGCCG 1 cut(s) 310
Eco57I CTGAAG 4 cut(s) 39, 442, 639, 704
EcoRI GAATTC 1 cut(s) 950
EcoRII CCWGG 2 cut(s) 262, 513
EcoRV GATATC 1 cut(s) 691
EcoT14I CCWWGG 1 cut(s) 1020
EgeI GGCGCC 1 cut(s) 261
EheI GGCGCC 1 cut(s) 261
ErhI CCWWGG 1 cut(s) 1020
FaeI CATG 5 cut(s) 361, 887, 935, 1036, 1101
FalI AAGNNNNNCTT 4 cut(s) 140, 172, 630, 662
FaqI GGGAC 1 cut(s) 926
FatI CATG 5 cut(s) 357, 883, 931, 1032, 1097
FauI CCCGC 3 cut(s) 104, 151, 192
FblI GTMKAC 1 cut(s) 936
Fnu4HI GCNGC 5 cut(s) 47, 532, 894, 1029, 1077
FokI GGATG 4 cut(s) 354, 530, 644, 852
Fsp4HI GCNGC 5 cut(s) 47, 532, 894, 1029, 1077
GlaI GCGC 5 cut(s) 100, 171, 229, 261, 1037
GluI GCNGC 5 cut(s) 47, 532, 894, 1029, 1077
HaeII RGCGCY 2 cut(s) 231, 263
HaeIII GGCC 4 cut(s) 141, 312, 531, 896
HapII CCGG 3 cut(s) 138, 222, 404
HgaI GACGC 1 cut(s) 115
HhaI GCGC 5 cut(s) 101, 172, 230, 262, 1038
Hin1I GRCGYC 2 cut(s) 260, 673
Hin1II CATG 5 cut(s) 361, 887, 935, 1036, 1101
Hin6I GCGC 5 cut(s) 99, 170, 228, 260, 1036
HinP1I GCGC 5 cut(s) 99, 170, 228, 260, 1036
HindIII AAGCTT 1 cut(s) 21
HinfI GANTC 5 cut(s) 38, 281, 350, 604, 709
HpaII CCGG 3 cut(s) 138, 222, 404
HphI GGTGA 2 cut(s) 543, 1086
Hpy166II GTNNAC 4 cut(s) 285, 505, 680, 937
Hpy188I TCNGA 6 cut(s) 433, 609, 695, 723, 956, 1044
Hpy188III TCNNGA 3 cut(s) 108, 214, 395
Hpy8I GTNNAC 4 cut(s) 285, 505, 680, 937
Hpy99I CGWCG 3 cut(s) 177, 292, 678
HpyAV CCTTC 5 cut(s) 75, 166, 517, 798, 1049
HpyCH4III ACNGT 4 cut(s) 437, 591, 737, 985
HpyCH4IV ACGT 2 cut(s) 175, 673
HpyCH4V TGCA 4 cut(s) 386, 669, 760, 1079
HpyF10VI GCNNNNNNNGC 7 cut(s) 138, 143, 152, 227, 766, 893, 1044
HpyF3I CTNAG 2 cut(s) 42, 1043
HpySE526I ACGT 2 cut(s) 175, 673
Hsp92I GRCGYC 2 cut(s) 260, 673
Hsp92II CATG 5 cut(s) 361, 887, 935, 1036, 1101
HspAI GCGC 5 cut(s) 99, 170, 228, 260, 1036
KasI GGCGCC 1 cut(s) 259
KroI GCCGGC 1 cut(s) 137
KroNI GCCGGC 1 cut(s) 139
Kzo9I GATC 5 cut(s) 104, 150, 400, 584, 924
LmnI GCTCC 1 cut(s) 828
Lsp1109I GCAGC 3 cut(s) 58, 1015, 1063
LweI GCATC 1 cut(s) 874
MaeII ACGT 2 cut(s) 175, 673
MaeIII GTNAC 3 cut(s) 176, 549, 1061
MalI GATC 5 cut(s) 106, 152, 402, 586, 926
MbiI CCGCTC 1 cut(s) 187
MboI GATC 5 cut(s) 104, 150, 400, 584, 924
MboII GAAGA 8 cut(s) 60, 63, 66, 69, 72, 75, 78, 202
MflI RGATCY 2 cut(s) 104, 400
MluCI AATT 5 cut(s) 111, 235, 472, 950, 976
Mly113I GGCGCC 1 cut(s) 260
MlyI GAGTC 1 cut(s) 290
MnlI CCTC 9 cut(s) 65, 191, 285, 366, 391, 437, 520, 909, 1042
MroNI GCCGGC 1 cut(s) 137
MseI TTAA 3 cut(s) 714, 812, 1085
MslI CAYNNNNRTG 3 cut(s) 335, 888, 1096
MspA1I CMGCKG 1 cut(s) 1076
MspI CCGG 3 cut(s) 138, 222, 404
MspR9I CCNGG 2 cut(s) 264, 515
Mva1269I GAATGC 1 cut(s) 223
MvaI CCWGG 2 cut(s) 264, 515
MvnI CGCG 4 cut(s) 99, 101, 170, 172
MwoI GCNNNNNNNGC 7 cut(s) 138, 143, 152, 227, 766, 893, 1044
NaeI GCCGGC 1 cut(s) 139
NarI GGCGCC 1 cut(s) 260
NdeII GATC 5 cut(s) 104, 150, 400, 584, 924
NgoMIV GCCGGC 1 cut(s) 137
NlaIII CATG 5 cut(s) 361, 887, 935, 1036, 1101
NlaIV GGNNCC 5 cut(s) 6, 142, 261, 402, 897
NmeAIII GCCGAG 1 cut(s) 236
NmuCI GTSAC 3 cut(s) 176, 549, 1061
PctI GAATGC 1 cut(s) 223
PdiI GCCGGC 1 cut(s) 139
PfeI GAWTC 4 cut(s) 38, 350, 604, 709
PflFI GACNNNGTC 2 cut(s) 292, 675
PfoI TCCNGGA 1 cut(s) 513
PkrI GCNGC 5 cut(s) 48, 533, 895, 1030, 1078
PleI GAGTC 1 cut(s) 289
PluTI GGCGCC 1 cut(s) 263
PpsI GAGTC 1 cut(s) 289
Psp6I CCWGG 2 cut(s) 262, 513
PspGI CCWGG 2 cut(s) 262, 513
PspN4I GGNNCC 5 cut(s) 6, 142, 261, 402, 897
PspPI GGNCC 3 cut(s) 140, 529, 895
PstNI CAGNNNCTG 1 cut(s) 766
PsuI RGATCY 2 cut(s) 104, 400
PsyI GACNNNGTC 2 cut(s) 292, 675
PvuII CAGCTG 1 cut(s) 1076
RsaI GTAC 1 cut(s) 452
RsaNI GTAC 1 cut(s) 451
RseI CAYNNNNRTG 3 cut(s) 335, 888, 1096
SaqAI TTAA 3 cut(s) 714, 812, 1085
SatI GCNGC 5 cut(s) 47, 532, 894, 1029, 1077
Sau3AI GATC 5 cut(s) 104, 150, 400, 584, 924
Sau96I GGNCC 3 cut(s) 140, 529, 895
SchI GAGTC 1 cut(s) 290
ScrFI CCNGG 2 cut(s) 264, 515
SfaNI GCATC 1 cut(s) 874
SfoI GGCGCC 1 cut(s) 261
SmiMI CAYNNNNRTG 3 cut(s) 335, 888, 1096
SmlI CTYRAG 2 cut(s) 395, 797
SmoI CTYRAG 2 cut(s) 395, 797
Sse9I AATT 5 cut(s) 111, 235, 472, 950, 976
SsiI CCGC 7 cut(s) 97, 144, 185, 368, 532, 577, 893
SspDI GGCGCC 1 cut(s) 259
StyD4I CCNGG 2 cut(s) 262, 513
StyI CCWWGG 1 cut(s) 1020
TaaI ACNGT 4 cut(s) 437, 591, 737, 985
TaiI ACGT 2 cut(s) 178, 676
TaqI TCGA 6 cut(s) 109, 189, 213, 242, 276, 353
TasI AATT 5 cut(s) 111, 235, 472, 950, 976
TatI WGTACW 1 cut(s) 450
TauI GCSGC 2 cut(s) 534, 896
TfiI GAWTC 4 cut(s) 38, 350, 604, 709
Tru1I TTAA 3 cut(s) 714, 812, 1085
Tru9I TTAA 3 cut(s) 714, 812, 1085
TscAI CASTG 2 cut(s) 596, 771
TseFI GTSAC 3 cut(s) 176, 549, 1061
TseI GCWGC 3 cut(s) 46, 1028, 1076
Tsp45I GTSAC 3 cut(s) 176, 549, 1061
TspDTI ATGAA 3 cut(s) 78, 454, 651
TspRI CASTG 2 cut(s) 596, 771
Tth111I GACNNNGTC 2 cut(s) 292, 675
XapI RAATTY 4 cut(s) 111, 235, 950, 976
XcmI CCANNNNNNNNNTGG 1 cut(s) 570
XmiI GTMKAC 1 cut(s) 936
ZraI GACGTC 1 cut(s) 674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.