Rh5BG021500

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0002602
Physical Location & Seq
Forward (+)
1578243 .. 1581458
3216 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG021500.1

Sequence Viewer

Length: 1188 bp
ATGGTGACGATGACGAAGAAGACGTCGCCACCGTCAATCACGGCGCGCCACGTGATCAGGTCCAGCTGGAAGCTTCTGATCGTCTTCTCCGTCTTGCTCTGCGTTTTGGCTCTCTACAGGTTTCAGTCTCAGCAGCCTGACCTTTACTCTCCTTCTTCTTCTTCTTCTTCTTCTTCATTATCTCGCGCGAGATCTCGAATTGCTCGCCACAGCGTCAGCTTCGCCGGCCCCGCCAAGATCGCCTTCCTATTTCTCGCGCGACGTGACCTCCCGCTCGATTTTCTTTGGGAGAGCTTCTTCGAGAATGCCGGTGGCGCTTTGAATTTTTCGATTTATATTCACTCCGCGCCTGGTTTTGTGTTCGATGAGTCCACCACGAGGTCTCGCTTCTTTCACGGCCGTCAATTGCCGAATAGCATACAGGTGGGATGGGGAGAATCGAGCATGATTGAAGCGGAGAGGTTGTTGTTTGCGACAGCTCTTGAGGATCCAGCAAATCAAAGATTTGTTCTCCTCTCTGACAGTTGCGTTCCTTTGTACAACTTCAGCTTCATATACAATTACCTGATGGCTTCTCCTAGGAGTATTGTGGACAGCTTCCTGGATGTGAAGGAGGGCCGCTACAACCCAAAAATGTCACCCACAATACCGAAAGCCAAATGGCGGAAAGGATCACAGTGGATTGCTTTGATTCGGAGACACGCCGAAGTTCTTGTGGATGATGAAGTGATACTTCCGGTATTCAAAAAGTTTTGCAGGCGTCGCCCACCTGTTGATATCAGAAAGGGGAAGCTGAATCTTAAACTTCAGAAACAGCACAACTGTATCCCAGACGAACACTATGTGCAGACGCTGCTTGCGATGAGTGAGCTTGACGGCGAACTTGAGCGAAGGACATTAACCTATACATTGTGGAGCAACTCTCCAAATAAAACAGAGAGTAAAGGCTGGCATCCTATGACTTTCAACCATGCCAATGCGGCCCCTCAAAAGATAAGGGCAATAAAGGAGATCAGCCATGTATACTATGAGACAGAATTCCGAACAGAATGGTGCCGTATAAATTCAACTGCAGTTCCCTGTTTTTTGTTCGCAAGGAAGTTCTCCAAGGGTGCTGCAATGCGCCTTCTGAGCGAGGGAGTTGTTGGTCACTTTAACACAGCCACATTGTTAAAACCATCACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

395

Amino Acids

45.09

Weight (kDa)

9.79

Isoelectric Point (pI)

55.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 80 - 335 2.6e-68 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 26
AccB1I GGYRCC 1 cut(s) 1053
AccBSI CCGCTC 1 cut(s) 274
AccI GTMKAC 1 cut(s) 1023
AccII CGCG 6 cut(s) 46, 186, 188, 257, 259, 347
AciI CCGC 7 cut(s) 231, 272, 345, 455, 619, 664, 980
AclWI GGATC 3 cut(s) 482, 495, 679
AcoI YGGCCR 1 cut(s) 397
AcsI RAATTY 3 cut(s) 322, 1037, 1063
AcuI CTGAAG 2 cut(s) 529, 791
AcvI CACGTG 1 cut(s) 52
AcyI GRCGYC 2 cut(s) 23, 760
AdeI CACNNNGTG 1 cut(s) 844
AfaI GTAC 1 cut(s) 539
AfiI CCNNNNNNNGG 2 cut(s) 378, 663
AgsI TTSAA 5 cut(s) 322, 452, 745, 967, 1068
AjiI CACGTC 1 cut(s) 263
AjnI CCWGG 2 cut(s) 349, 600
AjuI GAANNNNNNNTTGG 2 cut(s) 227, 259
AluBI AGCT 9 cut(s) 66, 73, 219, 294, 479, 549, 597, 793, 871
AluI AGCT 9 cut(s) 66, 73, 219, 294, 479, 549, 597, 793, 871
Alw26I GTCTC 4 cut(s) 132, 387, 691, 1025
AlwI GGATC 3 cut(s) 482, 495, 679
AlwNI CAGNNNCTG 1 cut(s) 853
AoxI GGCC 4 cut(s) 226, 397, 616, 981
ApeKI GCWGC 3 cut(s) 133, 853, 1115
ApoI RAATTY 3 cut(s) 322, 1037, 1063
ArsI GACNNNNNNTTYG 2 cut(s) 8, 40
AscI GGCGCGCC 1 cut(s) 44
AspA2I CCTAGG 1 cut(s) 578
AspLEI GCGC 7 cut(s) 46, 48, 188, 259, 317, 349, 1125
AspS9I GGNCC 4 cut(s) 60, 227, 616, 982
AsuHPI GGTGA 3 cut(s) 16, 630, 1173
AvaII GGWCC 1 cut(s) 60
AvrII CCTAGG 1 cut(s) 578
BaeI ACNNNNGTAYC 2 cut(s) 808, 841
BamHI GGATCC 1 cut(s) 487
BanI GGYRCC 1 cut(s) 1053
BauI CACGAG 1 cut(s) 376
BbrPI CACGTG 1 cut(s) 52
BbsI GAAGAC 2 cut(s) 26, 76
BbvI GCAGC 3 cut(s) 145, 840, 1102
BccI CCATC 3 cut(s) 423, 562, 1186
BceAI ACGGC 5 cut(s) 57, 384, 412, 892, 1041
BciT130I CCWGG 2 cut(s) 351, 602
BciVI GTATCC 1 cut(s) 836
BclI TGATCA 1 cut(s) 54
BcoDI GTCTC 4 cut(s) 132, 387, 691, 1025
BfaI CTAG 1 cut(s) 579
BfmI CTRYAG 2 cut(s) 115, 1071
BfoI RGCGCY 1 cut(s) 318
BfuI GTATCC 1 cut(s) 836
BglI GCCNNNNNGGC 1 cut(s) 980
BglII AGATCT 1 cut(s) 191
BisI GCNGC 5 cut(s) 134, 619, 854, 981, 1116
BlnI CCTAGG 1 cut(s) 578
BlsI GCNGC 5 cut(s) 135, 620, 855, 982, 1117
Bme1390I CCNGG 2 cut(s) 351, 602
Bme18I GGWCC 1 cut(s) 60
BmgBI CACGTC 1 cut(s) 263
BmgT120I GGNCC 4 cut(s) 60, 227, 616, 982
BmiI GGNNCC 4 cut(s) 229, 489, 984, 1055
BmrFI CCNGG 2 cut(s) 351, 602
BmsI GCATC 1 cut(s) 961
BpiI GAAGAC 2 cut(s) 26, 76
BplI GAGNNNNNCTC 2 cut(s) 907, 939
BpuEI CTTGAG 2 cut(s) 503, 905
BsaAI YACGTR 1 cut(s) 52
BsaHI GRCGYC 2 cut(s) 23, 760
BsaI GGTCTC 1 cut(s) 387
BsaJI CCNNGG 2 cut(s) 578, 1107
BsaWI WCCGGW 1 cut(s) 736
BsaXI ACNNNNNCTCC 4 cut(s) 252, 282, 449, 479
Bsc4I CCNNNNNNNGG 2 cut(s) 378, 663
Bse118I RCCGGY 2 cut(s) 224, 308
Bse3DI GCAATG 1 cut(s) 1125
BseBI CCWGG 2 cut(s) 351, 602
BseDI CCNNGG 2 cut(s) 578, 1107
BseGI GGATG 4 cut(s) 434, 610, 724, 952
BseLI CCNNNNNNNGG 2 cut(s) 378, 663
BseMI GCAATG 1 cut(s) 1125
BseMII CTCAG 2 cut(s) 143, 1121
BsePI GCGCGC 1 cut(s) 44
BseRI GAGGAG 1 cut(s) 503
BseX3I CGGCCG 1 cut(s) 397
BseXI GCAGC 3 cut(s) 145, 840, 1102
BsgI GTGCAG 1 cut(s) 866
Bsh1236I CGCG 6 cut(s) 46, 186, 188, 257, 259, 347
Bsh1285I CGRYCG 1 cut(s) 400
BshFI GGCC 4 cut(s) 228, 399, 618, 983
BshNI GGYRCC 1 cut(s) 1053
BsiEI CGRYCG 1 cut(s) 400
BsiSI CCGG 3 cut(s) 225, 309, 737
BslI CCNNNNNNNGG 2 cut(s) 378, 663
BsmAI GTCTC 4 cut(s) 132, 387, 691, 1025
BsmI GAATGC 1 cut(s) 310
BsnI GGCC 4 cut(s) 228, 399, 618, 983
Bso31I GGTCTC 1 cut(s) 387
Bsp1407I TGTACA 1 cut(s) 537
Bsp143I GATC 7 cut(s) 54, 78, 191, 237, 487, 671, 1011
BspACI CCGC 7 cut(s) 231, 272, 345, 455, 619, 664, 980
BspANI GGCC 4 cut(s) 228, 399, 618, 983
BspCNI CTCAG 2 cut(s) 142, 1122
BspFNI CGCG 6 cut(s) 46, 186, 188, 257, 259, 347
BspLI GGNNCC 4 cut(s) 229, 489, 984, 1055
BspMAI CTGCAG 1 cut(s) 1075
BspPI GGATC 3 cut(s) 482, 495, 679
BspT107I GGYRCC 1 cut(s) 1053
BspTNI GGTCTC 1 cut(s) 387
BsrBI CCGCTC 1 cut(s) 274
BsrDI GCAATG 1 cut(s) 1125
BsrFI RCCGGY 2 cut(s) 224, 308
BsrGI TGTACA 1 cut(s) 537
BssAI RCCGGY 2 cut(s) 224, 308
BssECI CCNNGG 2 cut(s) 578, 1107
BssHII GCGCGC 1 cut(s) 44
BssMI GATC 7 cut(s) 54, 78, 191, 237, 487, 671, 1011
BssNAI GTATAC 1 cut(s) 1024
BssNI GRCGYC 2 cut(s) 23, 760
BssSI CACGAG 1 cut(s) 376
BssT1I CCWWGG 2 cut(s) 578, 1107
Bst1107I GTATAC 1 cut(s) 1024
Bst2BI CACGAG 1 cut(s) 376
Bst2UI CCWGG 2 cut(s) 351, 602
Bst4CI ACNGT 4 cut(s) 33, 524, 678, 824
BstACI GRCGYC 2 cut(s) 23, 760
BstAPI GCANNNNNTGC 1 cut(s) 853
BstAUI TGTACA 1 cut(s) 537
BstBAI YACGTR 1 cut(s) 52
BstC8I GCNNGC 6 cut(s) 46, 205, 226, 758, 858, 950
BstDEI CTNAG 2 cut(s) 129, 1130
BstF5I GGATG 4 cut(s) 434, 610, 724, 952
BstFNI CGCG 6 cut(s) 46, 186, 188, 257, 259, 347
BstH2I RGCGCY 1 cut(s) 318
BstHHI GCGC 7 cut(s) 46, 48, 188, 259, 317, 349, 1125
BstKTI GATC 7 cut(s) 57, 81, 194, 240, 490, 674, 1014
BstMAI GTCTC 4 cut(s) 132, 387, 691, 1025
BstMBI GATC 7 cut(s) 54, 78, 191, 237, 487, 671, 1011
BstMCI CGRYCG 1 cut(s) 400
BstMWI GCNNNNNNNGC 8 cut(s) 225, 230, 239, 314, 762, 853, 980, 1131
BstNI CCWGG 2 cut(s) 351, 602
BstSCI CCNGG 2 cut(s) 349, 600
BstSFI CTRYAG 2 cut(s) 115, 1071
BstUI CGCG 6 cut(s) 46, 186, 188, 257, 259, 347
BstV1I GCAGC 3 cut(s) 145, 840, 1102
BstV2I GAAGAC 2 cut(s) 26, 76
BstX2I RGATCY 2 cut(s) 191, 487
BstYI RGATCY 2 cut(s) 191, 487
BstZ17I GTATAC 1 cut(s) 1024
BstZI CGGCCG 1 cut(s) 397
BsuI GTATCC 1 cut(s) 836
BsuRI GGCC 4 cut(s) 228, 399, 618, 983
BtgZI GCGATG 1 cut(s) 875
BtrI CACGTC 1 cut(s) 263
BtsCI GGATG 4 cut(s) 434, 610, 724, 952
BtsIMutI CAGTG 1 cut(s) 683
Cac8I GCNNGC 6 cut(s) 46, 205, 226, 758, 858, 950
CaiI CAGNNNCTG 1 cut(s) 853
CfoI GCGC 7 cut(s) 46, 48, 188, 259, 317, 349, 1125
Cfr10I RCCGGY 2 cut(s) 224, 308
Cfr13I GGNCC 4 cut(s) 60, 227, 616, 982
CseI GACGC 3 cut(s) 202, 749, 859
Csp6I GTAC 1 cut(s) 538
CviAII CATG 4 cut(s) 445, 971, 1019, 1185
CviQI GTAC 1 cut(s) 538
DdeI CTNAG 2 cut(s) 129, 1130
DpnI GATC 7 cut(s) 56, 80, 193, 239, 489, 673, 1013
DpnII GATC 7 cut(s) 54, 78, 191, 237, 487, 671, 1011
DraIII CACNNNGTG 1 cut(s) 844
EaeI YGGCCR 1 cut(s) 397
EagI CGGCCG 1 cut(s) 397
EciI GGCGGA 1 cut(s) 679
EclXI CGGCCG 1 cut(s) 397
Eco130I CCWWGG 2 cut(s) 578, 1107
Eco31I GGTCTC 1 cut(s) 387
Eco32I GATATC 1 cut(s) 778
Eco47I GGWCC 1 cut(s) 60
Eco52I CGGCCG 1 cut(s) 397
Eco57I CTGAAG 2 cut(s) 529, 791
Eco72I CACGTG 1 cut(s) 52
EcoRI GAATTC 1 cut(s) 1037
EcoRII CCWGG 2 cut(s) 349, 600
EcoRV GATATC 1 cut(s) 778
EcoT14I CCWWGG 2 cut(s) 578, 1107
ErhI CCWWGG 2 cut(s) 578, 1107
FaeI CATG 4 cut(s) 448, 974, 1022, 1188
FalI AAGNNNNNCTT 4 cut(s) 227, 259, 717, 749
FatI CATG 4 cut(s) 444, 970, 1018, 1184
FauI CCCGC 2 cut(s) 238, 279
FbaI TGATCA 1 cut(s) 54
FblI GTMKAC 1 cut(s) 1023
Fnu4HI GCNGC 5 cut(s) 134, 619, 854, 981, 1116
FokI GGATG 4 cut(s) 441, 617, 731, 939
Fsp4HI GCNGC 5 cut(s) 134, 619, 854, 981, 1116
FspBI CTAG 1 cut(s) 579
GlaI GCGC 7 cut(s) 45, 47, 187, 258, 316, 348, 1124
GluI GCNGC 5 cut(s) 134, 619, 854, 981, 1116
HaeII RGCGCY 1 cut(s) 318
HaeIII GGCC 4 cut(s) 228, 399, 618, 983
HapII CCGG 3 cut(s) 225, 309, 737
HgaI GACGC 3 cut(s) 202, 749, 859
HhaI GCGC 7 cut(s) 46, 48, 188, 259, 317, 349, 1125
Hin1I GRCGYC 2 cut(s) 23, 760
Hin1II CATG 4 cut(s) 448, 974, 1022, 1188
Hin6I GCGC 7 cut(s) 44, 46, 186, 257, 315, 347, 1123
HinP1I GCGC 7 cut(s) 44, 46, 186, 257, 315, 347, 1123
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 4 cut(s) 368, 437, 691, 796
HpaII CCGG 3 cut(s) 225, 309, 737
HphI GGTGA 3 cut(s) 16, 630, 1173
Hpy166II GTNNAC 3 cut(s) 372, 592, 1024
Hpy188I TCNGA 7 cut(s) 78, 520, 696, 782, 810, 1043, 1131
Hpy188III TCNNGA 3 cut(s) 195, 301, 482
Hpy8I GTNNAC 3 cut(s) 372, 592, 1024
Hpy99I CGWCG 3 cut(s) 28, 264, 765
HpyAV CCTTC 5 cut(s) 162, 253, 604, 885, 1136
HpyCH4III ACNGT 4 cut(s) 33, 524, 678, 824
HpyCH4IV ACGT 3 cut(s) 23, 51, 262
HpyCH4V TGCA 4 cut(s) 756, 847, 1073, 1118
HpyF10VI GCNNNNNNNGC 8 cut(s) 225, 230, 239, 314, 762, 853, 980, 1131
HpyF3I CTNAG 2 cut(s) 129, 1130
HpySE526I ACGT 3 cut(s) 23, 51, 262
Hsp92I GRCGYC 2 cut(s) 23, 760
Hsp92II CATG 4 cut(s) 448, 974, 1022, 1188
HspAI GCGC 7 cut(s) 44, 46, 186, 257, 315, 347, 1123
KroI GCCGGC 1 cut(s) 224
KroNI GCCGGC 1 cut(s) 226
Ksp22I TGATCA 1 cut(s) 54
Kzo9I GATC 7 cut(s) 54, 78, 191, 237, 487, 671, 1011
LmnI GCTCC 1 cut(s) 915
Lsp1109I GCAGC 3 cut(s) 145, 840, 1102
LweI GCATC 1 cut(s) 961
MaeI CTAG 1 cut(s) 579
MaeII ACGT 3 cut(s) 23, 51, 262
MaeIII GTNAC 4 cut(s) 4, 263, 636, 1148
MalI GATC 7 cut(s) 56, 80, 193, 239, 489, 673, 1013
MbiI CCGCTC 1 cut(s) 274
MboI GATC 7 cut(s) 54, 78, 191, 237, 487, 671, 1011
MfeI CAATTG 1 cut(s) 404
MflI RGATCY 2 cut(s) 191, 487
MluCI AATT 6 cut(s) 198, 322, 404, 559, 1037, 1063
MlyI GAGTC 1 cut(s) 377
MnlI CCTC 8 cut(s) 278, 372, 453, 478, 524, 607, 996, 1129
MroNI GCCGGC 1 cut(s) 224
MseI TTAA 4 cut(s) 801, 899, 1155, 1172
MslI CAYNNNNRTG 3 cut(s) 422, 975, 1183
MspA1I CMGCKG 1 cut(s) 66
MspI CCGG 3 cut(s) 225, 309, 737
MspR9I CCNGG 2 cut(s) 351, 602
MunI CAATTG 1 cut(s) 404
Mva1269I GAATGC 1 cut(s) 310
MvaI CCWGG 2 cut(s) 351, 602
MvnI CGCG 6 cut(s) 46, 186, 188, 257, 259, 347
MwoI GCNNNNNNNGC 8 cut(s) 225, 230, 239, 314, 762, 853, 980, 1131
NaeI GCCGGC 1 cut(s) 226
NdeII GATC 7 cut(s) 54, 78, 191, 237, 487, 671, 1011
NgoMIV GCCGGC 1 cut(s) 224
NlaIII CATG 4 cut(s) 448, 974, 1022, 1188
NlaIV GGNNCC 4 cut(s) 229, 489, 984, 1055
NmuCI GTSAC 4 cut(s) 4, 263, 636, 1148
PalAI GGCGCGCC 1 cut(s) 44
PauI GCGCGC 1 cut(s) 44
PcsI WCGNNNNNNNCGW 4 cut(s) 20, 29, 87, 857
PctI GAATGC 1 cut(s) 310
PdiI GCCGGC 1 cut(s) 226
PfeI GAWTC 3 cut(s) 437, 691, 796
PfoI TCCNGGA 1 cut(s) 600
PkrI GCNGC 5 cut(s) 135, 620, 855, 982, 1117
PleI GAGTC 1 cut(s) 376
PmaCI CACGTG 1 cut(s) 52
PmlI CACGTG 1 cut(s) 52
PpsI GAGTC 1 cut(s) 376
Ppu21I YACGTR 1 cut(s) 52
Psp6I CCWGG 2 cut(s) 349, 600
PspCI CACGTG 1 cut(s) 52
PspGI CCWGG 2 cut(s) 349, 600
PspN4I GGNNCC 4 cut(s) 229, 489, 984, 1055
PspPI GGNCC 4 cut(s) 60, 227, 616, 982
PstI CTGCAG 1 cut(s) 1075
PstNI CAGNNNCTG 1 cut(s) 853
PsuI RGATCY 2 cut(s) 191, 487
PteI GCGCGC 1 cut(s) 44
PvuII CAGCTG 1 cut(s) 66
RsaI GTAC 1 cut(s) 539
RsaNI GTAC 1 cut(s) 538
RseI CAYNNNNRTG 3 cut(s) 422, 975, 1183
SaqAI TTAA 4 cut(s) 801, 899, 1155, 1172
SatI GCNGC 5 cut(s) 134, 619, 854, 981, 1116
Sau3AI GATC 7 cut(s) 54, 78, 191, 237, 487, 671, 1011
Sau96I GGNCC 4 cut(s) 60, 227, 616, 982
SchI GAGTC 1 cut(s) 377
ScrFI CCNGG 2 cut(s) 351, 602
SfaNI GCATC 1 cut(s) 961
SfcI CTRYAG 2 cut(s) 115, 1071
SgsI GGCGCGCC 1 cut(s) 44
SinI GGWCC 1 cut(s) 60
SmiMI CAYNNNNRTG 3 cut(s) 422, 975, 1183
SmlI CTYRAG 2 cut(s) 482, 884
SmoI CTYRAG 2 cut(s) 482, 884
Sse9I AATT 6 cut(s) 198, 322, 404, 559, 1037, 1063
SsiI CCGC 7 cut(s) 231, 272, 345, 455, 619, 664, 980
SspMI CTAG 1 cut(s) 579
StyD4I CCNGG 2 cut(s) 349, 600
StyI CCWWGG 2 cut(s) 578, 1107
TaaI ACNGT 4 cut(s) 33, 524, 678, 824
TaiI ACGT 3 cut(s) 26, 54, 265
TaqI TCGA 6 cut(s) 196, 276, 300, 329, 363, 440
TasI AATT 6 cut(s) 198, 322, 404, 559, 1037, 1063
TatI WGTACW 1 cut(s) 537
TauI GCSGC 2 cut(s) 621, 983
TfiI GAWTC 3 cut(s) 437, 691, 796
Tru1I TTAA 4 cut(s) 801, 899, 1155, 1172
Tru9I TTAA 4 cut(s) 801, 899, 1155, 1172
TscAI CASTG 1 cut(s) 683
TseFI GTSAC 4 cut(s) 4, 263, 636, 1148
TseI GCWGC 3 cut(s) 133, 853, 1115
Tsp45I GTSAC 4 cut(s) 4, 263, 636, 1148
TspDTI ATGAA 3 cut(s) 165, 541, 738
TspGWI ACGGA 1 cut(s) 79
TspRI CASTG 1 cut(s) 683
VpaK11BI GGWCC 1 cut(s) 60
XapI RAATTY 3 cut(s) 322, 1037, 1063
XmaJI CCTAGG 1 cut(s) 578
XmiI GTMKAC 1 cut(s) 1023
XspI CTAG 1 cut(s) 579
ZraI GACGTC 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.