Rorug02G0470900

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002602
Physical Location & Seq
Forward (+)
60176828 .. 60181716
4889 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0470900.1

Sequence Viewer

Length: 1641 bp
ATGCTATCTACACTCCAACCTCCGCGCTCACTCTCTCACTTCCGCACTAAACCCCTCCTCCTCCTCCGCCGCCACACCTCCCTTAAACCCCTCTCCTCCTTATCCTCACAGCCCGTCACCCACTCCACCCTCACAGACCAATCCAAATCCCAAATCCTCTCCACCCGCGACTCCCTCCTCTCCCGCCAAATCACCGCCGCCGAGCTCGCCGAGTATTTCCTCGCCCGCCTCCGCCGCACCGAGCCCCACCTCCGCTCCTTCCTCCACGTCTCCGACGCCGTCCTCGCCCAGGCCCGCGACCTCGACGACAAGCTTCGCCGGAATGAGGAGGTGGGACCCCTGGCCGGGGTCCTTGTGGCGGTGAAGGATAACATATGTACGGCGGAGATGCCGTCCACGGCGGGTTCCAGGGTTTTGGAGGGCTATACGCCGCCGTATGATGCCACCGCCGTCAGGAAGATTAAGGAGCTGGGTGGGATTGTGGTGGGCAAGACTAATTTGGATGAGTTTGGGATGGGTAGCACCACCGAAGGCTCCGCGTTTCAGGTGACTGCAAACCCATGGGACACGACACGGGTGCCAGGAGGGTCATCAGGAGGTTCGGCTGCAGCTGTATCTGCTAGGCAGAGTATGGTATCATTGGGAAGTGATACTGGTGGAAGTGTGAGGCAGCCAGCATCGTTTTGTGGTGTTGTTGGGTTGAAGCCCACGTATGGGCGTGTATCGAGGTTTGGACTTATGGCATATGCATCATCTCTTGATGTTATTGGCTGCTTTGGAACATCTGTTGCTGATGCTGGGATTCTTCTTCATGCAATTTCTGGTCATGATAGACTTGATGCTACCAGCAGCAAGCGTGAGGTTCCCGACCTTGCATCTCAATTTGTTTCCATTAGTTTGCTAGAGTCTAAACCTCTGAAAGGACTAAAAATTGGTCTGATCCGTGAAACTCTCGATGATGGTGTTGATATTGGAGTAACTTCTGCAATTCGTACTGCTGCACTGCATCTTGAAGAATTAGGATGCTCCGTAACAGAGGTCTCATTACCATCTTTCTCACTTGGATTGCCAGCCTACTATGTTATTGCTTCATCCGAGTCATCTTCAAACTTATCGCGTTATGACGGTCTCAGGTATGGAAACCAAGTTGCTGCTGATGAGCTGAACTCGCAATACATGGACTCCCGCGCCAAGGGATTTGGTTCTGAGGTCAAAAGGAGAATTCTAACCGGAACATATGCCCTTTCAGCTGGTTATTATGATGCATATTATAAACGCGCCCAGCAGGTGAGGACGATAATTCGGAAAAGCTTCAAGGCGGTACTGGACGAACATGACATCTTAATCTCACCTGCTGCTCCATCTGCTGCGTATAAGATTGGTGAAAAGAAAAATGATCCGTTAGCAATGTATGCAGGCGACATAATGACGGTTAATGTTAACTTGGCAGGTCTACCTGCATTGGTTCTGCCATGTGGATTTGCCGAAGGGGGGTCAGCAGGCCTTCCGGTTGGTCTTCAAATGATTGGTGCAGCATTCGATGAGGAAAAGTTGCTTAGAGTGGGTCACATCTTTGAGCAGACCCTTCAAAATTGCAGATTTGTACCTCCATTGATAGCAGATGAGGATGTACCATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

546

Amino Acids

58.36

Weight (kDa)

7.14

Isoelectric Point (pI)

46.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 68 - 519 3.5e-143 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1272
AarI CACCTGC 2 cut(s) 1276, 1360
Acc36I ACCTGC 4 cut(s) 1276, 1360, 1439, 1465
AccB1I GGYRCC 1 cut(s) 577
AccBSI CCGCTC 1 cut(s) 255
AccI GTMKAC 1 cut(s) 1453
AccII CGCG 7 cut(s) 25, 168, 297, 539, 1117, 1188, 1278
AclWI GGATC 2 cut(s) 934, 1391
AcoI YGGCCR 1 cut(s) 342
AcsI RAATTY 1 cut(s) 1221
AcyI GRCGYC 1 cut(s) 276
AfaI GTAC 5 cut(s) 379, 994, 1323, 1605, 1632
AgsI TTSAA 6 cut(s) 703, 1013, 1107, 1315, 1520, 1589
AjiI CACGTC 1 cut(s) 268
AjnI CCWGG 4 cut(s) 288, 339, 407, 580
AluBI AGCT 7 cut(s) 205, 313, 469, 611, 1162, 1250, 1311
AluI AGCT 7 cut(s) 205, 313, 469, 611, 1162, 1250, 1311
Alw21I GWGCWC 1 cut(s) 207
Alw26I GTCTC 3 cut(s) 274, 1045, 1133
AlwI GGATC 2 cut(s) 934, 1391
AoxI GGCC 3 cut(s) 291, 342, 1501
ApoI RAATTY 1 cut(s) 1221
Asp700I GAANNNNTTC 1 cut(s) 1310
AspLEI GCGC 3 cut(s) 27, 1190, 1280
AspS9I GGNCC 3 cut(s) 292, 335, 349
AsuC2I CCSGG 1 cut(s) 346
AsuHPI GGTGA 7 cut(s) 109, 184, 373, 559, 1300, 1341, 1394
AvaII GGWCC 2 cut(s) 335, 349
BanI GGYRCC 1 cut(s) 577
BanII GRGCYC 2 cut(s) 207, 246
BbsI GAAGAC 1 cut(s) 1508
Bbv12I GWGCWC 1 cut(s) 207
BccI CCATC 4 cut(s) 508, 953, 1057, 1369
BceAI ACGGC 6 cut(s) 263, 376, 396, 414, 418, 434
BcgI CGANNNNNNTGC 2 cut(s) 559, 593
BciT130I CCWGG 4 cut(s) 290, 341, 409, 582
BcnI CCSGG 1 cut(s) 346
BcoDI GTCTC 3 cut(s) 274, 1045, 1133
BfaI CTAG 3 cut(s) 621, 902, 1639
BfmI CTRYAG 1 cut(s) 606
BfuAI ACCTGC 4 cut(s) 1276, 1360, 1439, 1465
Bme1390I CCNGG 5 cut(s) 290, 341, 346, 409, 582
Bme18I GGWCC 2 cut(s) 335, 349
BmgBI CACGTC 1 cut(s) 268
BmgT120I GGNCC 3 cut(s) 292, 335, 349
BmiI GGNNCC 7 cut(s) 336, 337, 350, 406, 535, 579, 864
BmrFI CCNGG 5 cut(s) 290, 341, 346, 409, 582
BpiI GAAGAC 1 cut(s) 1508
BplI GAGNNNNNCTC 2 cut(s) 1151, 1183
BpuMI CCSGG 1 cut(s) 346
BsaAI YACGTR 1 cut(s) 711
BsaHI GRCGYC 1 cut(s) 276
BsaI GGTCTC 2 cut(s) 1045, 1133
BsaJI CCNNGG 7 cut(s) 288, 339, 345, 396, 408, 560, 1191
BsaWI WCCGGW 2 cut(s) 1229, 1507
BsaXI ACNNNNNCTCC 8 cut(s) 42, 72, 239, 269, 377, 407, 1166, 1196
Bse1I ACTGG 2 cut(s) 658, 1329
Bse3DI GCAATG 1 cut(s) 1413
BseBI CCWGG 4 cut(s) 290, 341, 409, 582
BseDI CCNNGG 7 cut(s) 288, 339, 345, 396, 408, 560, 1191
BseGI GGATG 5 cut(s) 508, 519, 1028, 1091, 1633
BseMI GCAATG 1 cut(s) 1413
BseMII CTCAG 2 cut(s) 1144, 1197
BseNI ACTGG 2 cut(s) 658, 1329
BseRI GAGGAG 6 cut(s) 47, 50, 53, 85, 167, 341
BseYI CCCAGC 3 cut(s) 469, 797, 1281
BsgI GTGCAG 2 cut(s) 984, 1551
Bsh1236I CGCG 7 cut(s) 25, 168, 297, 539, 1117, 1188, 1278
BshFI GGCC 3 cut(s) 293, 344, 1503
BshNI GGYRCC 1 cut(s) 577
BsiHKAI GWGCWC 1 cut(s) 207
BsiSI CCGG 4 cut(s) 319, 345, 1230, 1508
BslFI GGGAC 2 cut(s) 348, 578
BsmAI GTCTC 3 cut(s) 274, 1045, 1133
BsmBI CGTCTC 1 cut(s) 274
BsmFI GGGAC 2 cut(s) 348, 578
BsmI GAATGC 1 cut(s) 1535
BsnI GGCC 3 cut(s) 293, 344, 1503
Bso31I GGTCTC 2 cut(s) 1045, 1133
Bsp1286I GDGCHC 2 cut(s) 207, 246
Bsp143I GATC 2 cut(s) 939, 1396
Bsp19I CCATGG 1 cut(s) 560
BspANI GGCC 3 cut(s) 293, 344, 1503
BspCNI CTCAG 2 cut(s) 1143, 1198
BspFNI CGCG 7 cut(s) 25, 168, 297, 539, 1117, 1188, 1278
BspHI TCATGA 1 cut(s) 826
BspLI GGNNCC 7 cut(s) 336, 337, 350, 406, 535, 579, 864
BspMAI CTGCAG 1 cut(s) 610
BspMI ACCTGC 4 cut(s) 1276, 1360, 1439, 1465
BspPI GGATC 2 cut(s) 934, 1391
BspT107I GGYRCC 1 cut(s) 577
BspTNI GGTCTC 2 cut(s) 1045, 1133
BsrBI CCGCTC 1 cut(s) 255
BsrDI GCAATG 1 cut(s) 1413
BsrI ACTGG 2 cut(s) 658, 1329
BssECI CCNNGG 7 cut(s) 288, 339, 345, 396, 408, 560, 1191
BssMI GATC 2 cut(s) 939, 1396
BssNI GRCGYC 1 cut(s) 276
BssT1I CCWWGG 2 cut(s) 560, 1191
Bst2UI CCWGG 4 cut(s) 290, 341, 409, 582
Bst4CI ACNGT 2 cut(s) 1127, 1432
BstACI GRCGYC 1 cut(s) 276
BstAPI GCANNNNNTGC 1 cut(s) 1412
BstBAI YACGTR 1 cut(s) 711
BstC8I GCNNGC 8 cut(s) 207, 226, 295, 675, 854, 1071, 1417, 1501
BstDEI CTNAG 3 cut(s) 1130, 1206, 1556
BstDSI CCRYGG 2 cut(s) 396, 560
BstENI CCTNNNNNAGG 2 cut(s) 287, 918
BstF5I GGATG 5 cut(s) 508, 519, 1028, 1091, 1633
BstFNI CGCG 7 cut(s) 25, 168, 297, 539, 1117, 1188, 1278
BstHHI GCGC 3 cut(s) 27, 1190, 1280
BstKTI GATC 2 cut(s) 942, 1399
BstMAI GTCTC 3 cut(s) 274, 1045, 1133
BstMBI GATC 2 cut(s) 939, 1396
BstMWI GCNNNNNNNGC 8 cut(s) 206, 234, 284, 617, 1168, 1247, 1364, 1412
BstNI CCWGG 4 cut(s) 290, 341, 409, 582
BstSCI CCNGG 5 cut(s) 288, 339, 344, 407, 580
BstSFI CTRYAG 1 cut(s) 606
BstUI CGCG 7 cut(s) 25, 168, 297, 539, 1117, 1188, 1278
BstV2I GAAGAC 1 cut(s) 1508
BstXI CCANNNNNNTGG 1 cut(s) 415
BsuRI GGCC 3 cut(s) 293, 344, 1503
BtgI CCRYGG 2 cut(s) 396, 560
BtrI CACGTC 1 cut(s) 268
BtsCI GGATG 5 cut(s) 508, 519, 1028, 1091, 1633
BtsI GCAGTG 1 cut(s) 1001
BtsIMutI CAGTG 1 cut(s) 1001
BveI ACCTGC 4 cut(s) 1276, 1360, 1439, 1465
Cac8I GCNNGC 8 cut(s) 207, 226, 295, 675, 854, 1071, 1417, 1501
CciI TCATGA 1 cut(s) 826
CfoI GCGC 3 cut(s) 27, 1190, 1280
Cfr13I GGNCC 3 cut(s) 292, 335, 349
CseI GACGC 1 cut(s) 284
Csp6I GTAC 5 cut(s) 378, 993, 1322, 1604, 1631
CviAII CATG 7 cut(s) 561, 812, 827, 1177, 1334, 1473, 1635
CviQI GTAC 5 cut(s) 378, 993, 1322, 1604, 1631
DdeI CTNAG 3 cut(s) 1130, 1206, 1556
DpnI GATC 2 cut(s) 941, 1398
DpnII GATC 2 cut(s) 939, 1396
EaeI YGGCCR 1 cut(s) 342
EciI GGCGGA 3 cut(s) 56, 221, 398
Ecl136II GAGCTC 1 cut(s) 205
Eco130I CCWWGG 2 cut(s) 560, 1191
Eco147I AGGCCT 1 cut(s) 1503
Eco24I GRGCYC 2 cut(s) 207, 246
Eco31I GGTCTC 2 cut(s) 1045, 1133
Eco47I GGWCC 2 cut(s) 335, 349
Eco53kI GAGCTC 1 cut(s) 205
EcoICRI GAGCTC 1 cut(s) 205
EcoNI CCTNNNNNAGG 2 cut(s) 287, 918
EcoO109I RGGNCCY 2 cut(s) 335, 349
EcoRI GAATTC 1 cut(s) 1221
EcoRII CCWGG 4 cut(s) 288, 339, 407, 580
EcoT14I CCWWGG 2 cut(s) 560, 1191
EcoT22I ATGCAT 2 cut(s) 751, 1267
EcoT38I GRGCYC 2 cut(s) 207, 246
ErhI CCWWGG 2 cut(s) 560, 1191
Esp3I CGTCTC 1 cut(s) 274
FaeI CATG 7 cut(s) 564, 815, 830, 1180, 1337, 1476, 1638
FaqI GGGAC 2 cut(s) 348, 578
FatI CATG 7 cut(s) 560, 811, 826, 1176, 1333, 1472, 1634
FauI CCCGC 6 cut(s) 173, 191, 233, 302, 394, 1193
FauNDI CATATG 3 cut(s) 374, 745, 1237
FblI GTMKAC 1 cut(s) 1453
FokI GGATG 4 cut(s) 515, 526, 1035, 1078
FriOI GRGCYC 2 cut(s) 207, 246
FspBI CTAG 3 cut(s) 621, 902, 1639
GlaI GCGC 3 cut(s) 26, 1189, 1279
GsaI CCCAGC 3 cut(s) 473, 801, 1285
HaeIII GGCC 3 cut(s) 293, 344, 1503
HapII CCGG 4 cut(s) 319, 345, 1230, 1508
HgaI GACGC 1 cut(s) 284
HhaI GCGC 3 cut(s) 27, 1190, 1280
Hin1I GRCGYC 1 cut(s) 276
Hin1II CATG 7 cut(s) 564, 815, 830, 1180, 1337, 1476, 1638
Hin6I GCGC 3 cut(s) 25, 1188, 1278
HinP1I GCGC 3 cut(s) 25, 1188, 1278
HincII GTYRAC 1 cut(s) 1441
HindII GTYRAC 1 cut(s) 1441
HindIII AAGCTT 2 cut(s) 311, 1309
HinfI GANTC 5 cut(s) 170, 802, 905, 1097, 1181
HpaI GTTAAC 1 cut(s) 1441
HpaII CCGG 4 cut(s) 319, 345, 1230, 1508
HphI GGTGA 7 cut(s) 109, 184, 373, 559, 1300, 1341, 1394
Hpy166II GTNNAC 3 cut(s) 396, 1441, 1454
Hpy188I TCNGA 6 cut(s) 274, 918, 939, 1096, 1207, 1305
Hpy188III TCNNGA 7 cut(s) 454, 594, 758, 827, 866, 953, 1010
Hpy8I GTNNAC 3 cut(s) 396, 1441, 1454
Hpy99I CGWCG 2 cut(s) 278, 308
HpyAV CCTTC 6 cut(s) 268, 358, 524, 1481, 1514, 1595
HpyCH4III ACNGT 2 cut(s) 1127, 1432
HpyCH4IV ACGT 2 cut(s) 267, 710
HpyF10VI GCNNNNNNNGC 8 cut(s) 206, 234, 284, 617, 1168, 1247, 1364, 1412
HpyF3I CTNAG 3 cut(s) 1130, 1206, 1556
HpySE526I ACGT 2 cut(s) 267, 710
Hsp92I GRCGYC 1 cut(s) 276
Hsp92II CATG 7 cut(s) 564, 815, 830, 1180, 1337, 1476, 1638
HspAI GCGC 3 cut(s) 25, 1188, 1278
KflI GGGWCCC 1 cut(s) 335
KspAI GTTAAC 1 cut(s) 1441
Kzo9I GATC 2 cut(s) 939, 1396
LmnI GCTCC 5 cut(s) 260, 466, 539, 1031, 1363
MaeI CTAG 3 cut(s) 621, 902, 1639
MaeII ACGT 2 cut(s) 267, 710
MaeIII GTNAC 5 cut(s) 115, 547, 976, 1030, 1565
MalI GATC 2 cut(s) 941, 1398
MbiI CCGCTC 1 cut(s) 255
MboI GATC 2 cut(s) 939, 1396
MboII GAAGA 6 cut(s) 469, 797, 800, 1025, 1095, 1508
MhlI GDGCHC 2 cut(s) 207, 246
MluCI AATT 9 cut(s) 496, 816, 881, 930, 987, 1016, 1221, 1299, 1591
MlyI GAGTC 4 cut(s) 164, 914, 1106, 1175
MmeI TCCRAC 2 cut(s) 40, 297
Mph1103I ATGCAT 2 cut(s) 751, 1267
MroXI GAANNNNTTC 1 cut(s) 1310
MseI TTAA 5 cut(s) 84, 462, 1343, 1434, 1440
MspA1I CMGCKG 2 cut(s) 611, 1250
MspI CCGG 4 cut(s) 319, 345, 1230, 1508
MspR9I CCNGG 5 cut(s) 290, 341, 346, 409, 582
Mva1269I GAATGC 1 cut(s) 1535
MvaI CCWGG 4 cut(s) 290, 341, 409, 582
MvnI CGCG 7 cut(s) 25, 168, 297, 539, 1117, 1188, 1278
MwoI GCNNNNNNNGC 8 cut(s) 206, 234, 284, 617, 1168, 1247, 1364, 1412
NciI CCSGG 1 cut(s) 346
NcoI CCATGG 1 cut(s) 560
NdeI CATATG 3 cut(s) 374, 745, 1237
NdeII GATC 2 cut(s) 939, 1396
NlaIII CATG 7 cut(s) 564, 815, 830, 1180, 1337, 1476, 1638
NlaIV GGNNCC 7 cut(s) 336, 337, 350, 406, 535, 579, 864
NmeAIII GCCGAG 2 cut(s) 226, 235
NmuCI GTSAC 3 cut(s) 115, 547, 1565
NsiI ATGCAT 2 cut(s) 751, 1267
PagI TCATGA 1 cut(s) 826
PaqCI CACCTGC 2 cut(s) 1276, 1360
PceI AGGCCT 1 cut(s) 1503
PctI GAATGC 1 cut(s) 1535
PdmI GAANNNNTTC 1 cut(s) 1310
PfeI GAWTC 1 cut(s) 802
PflFI GACNNNGTC 1 cut(s) 278
PleI GAGTC 4 cut(s) 164, 913, 1105, 1175
PpsI GAGTC 4 cut(s) 164, 913, 1105, 1175
Ppu21I YACGTR 1 cut(s) 711
PpuMI RGGWCCY 2 cut(s) 335, 349
PsiI TTATAA 1 cut(s) 1272
Psp124BI GAGCTC 1 cut(s) 207
Psp5II RGGWCCY 2 cut(s) 335, 349
Psp6I CCWGG 4 cut(s) 288, 339, 407, 580
PspFI CCCAGC 3 cut(s) 469, 797, 1281
PspGI CCWGG 4 cut(s) 288, 339, 407, 580
PspN4I GGNNCC 7 cut(s) 336, 337, 350, 406, 535, 579, 864
PspPI GGNCC 3 cut(s) 292, 335, 349
PspPPI RGGWCCY 2 cut(s) 335, 349
PsrI GAACNNNNNNTAC 2 cut(s) 1157, 1189
PstI CTGCAG 1 cut(s) 610
PsyI GACNNNGTC 1 cut(s) 278
PvuII CAGCTG 2 cut(s) 611, 1250
RsaI GTAC 5 cut(s) 379, 994, 1323, 1605, 1632
RsaNI GTAC 5 cut(s) 378, 993, 1322, 1604, 1631
SacI GAGCTC 1 cut(s) 207
SaqAI TTAA 5 cut(s) 84, 462, 1343, 1434, 1440
Sau3AI GATC 2 cut(s) 939, 1396
Sau96I GGNCC 3 cut(s) 292, 335, 349
SchI GAGTC 4 cut(s) 164, 914, 1106, 1175
ScrFI CCNGG 5 cut(s) 290, 341, 346, 409, 582
SduI GDGCHC 2 cut(s) 207, 246
SfcI CTRYAG 1 cut(s) 606
SinI GGWCC 2 cut(s) 335, 349
Sse9I AATT 9 cut(s) 496, 816, 881, 930, 987, 1016, 1221, 1299, 1591
SseBI AGGCCT 1 cut(s) 1503
SspMI CTAG 3 cut(s) 621, 902, 1639
SstI GAGCTC 1 cut(s) 207
StuI AGGCCT 1 cut(s) 1503
StyD4I CCNGG 5 cut(s) 288, 339, 344, 407, 580
StyI CCWWGG 2 cut(s) 560, 1191
TaaI ACNGT 2 cut(s) 1127, 1432
TaiI ACGT 2 cut(s) 270, 713
TaqI TCGA 4 cut(s) 303, 725, 954, 1539
TasI AATT 9 cut(s) 496, 816, 881, 930, 987, 1016, 1221, 1299, 1591
TauI GCSGC 4 cut(s) 72, 200, 237, 433
TfiI GAWTC 1 cut(s) 802
Tru1I TTAA 5 cut(s) 84, 462, 1343, 1434, 1440
Tru9I TTAA 5 cut(s) 84, 462, 1343, 1434, 1440
TscAI CASTG 1 cut(s) 1008
TseFI GTSAC 3 cut(s) 115, 547, 1565
Tsp45I GTSAC 3 cut(s) 115, 547, 1565
TspDTI ATGAA 2 cut(s) 800, 1080
TspGWI ACGGA 3 cut(s) 932, 1018, 1389
TspRI CASTG 1 cut(s) 1008
Tth111I GACNNNGTC 1 cut(s) 278
VpaK11BI GGWCC 2 cut(s) 335, 349
XagI CCTNNNNNAGG 2 cut(s) 287, 918
XapI RAATTY 1 cut(s) 1221
XmiI GTMKAC 1 cut(s) 1453
XmnI GAANNNNTTC 1 cut(s) 1310
XspI CTAG 3 cut(s) 621, 902, 1639
Zsp2I ATGCAT 2 cut(s) 751, 1267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.