FvH4_3g13310

Belongs to the eukaryotic ribosomal protein eS4 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001275
Physical Location & Seq
Reverse (-)
7950421 .. 7952806
2386 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g13310.t1

Sequence Viewer

Length: 795 bp
ATGGCGCGAGGACTGAAGAAGCATTTGAAGAGGCTCAATGCCCCCAAGCATTGGATGCTTGACAAATTGGGTGGTGCATTTGCCCCCAAGCCCTCATCTGGACCTCACAAGTCGAGGGAGTGTCTTCCATTGATCATTATCTTGCGAAACCGGTTGAAGTATGCTCTGACATACCGTGAGGTCATTGCCATCTTGATGCAACGTCATGTTATGGTTGATGGGAAAGTTAGGACTGATAAGACTTATCCTTCTGGTTTCATGGATGTTGTCTCAATCCCCAAGACAAATGAGAATTTCCGTCTCCTTTATGACACCAAAGGACGGTTCCGTCTGCATTCAATCAGGGATGAAGAGGCAAAGTTCAAACTCTGCAAGGTCCGTTCTGTGCAGTTTGGCCAAAAGAATATCCCTTACATTAATACCTATGATGGGAGAACAATTCGTTACCCTGATCCTCTCATCAAGGCAAATGACACCATCAAGCTTGATTTGGAGACCAACAAGATTGTTGACTTCATCAAGTTCGATGTTGGTAATGTTGTCATGGTGACAGGTGGTAGGAACAGAGGGCGAGTTGGAGTAATCAAGAGCAGGGAGAAGCATAAGGGAAGCTTTGAGACTATTCACGTCCAGGACGCCACTGGACATGAATTTGCAACCCGTTTGGGGAATGTGTTCACAATTGGTAAGGGTACCAAGCCATGGGTAAGCCTTCCCAAGGGTAAGGGTATTAAGCTTACTATCATTGAGGAGGCCAAGAAGAGGCAAGCTGCCCAAGCCACAGCTGCAGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
40S_S4_C KOW RS4NT Ribosomal_S4e S4

Protein Analysis

265

Amino Acids

29.89

Weight (kDa)

10.25

Isoelectric Point (pI)

23.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RS4NT PF08071 3 - 39 2.3e-18 RS4NT (NUC023) domain
Ribosomal_S4e PF00900 95 - 169 4.4e-34 Ribosomal family S4e
KOW PF00467 177 - 210 8e-07 KOW motif
40S_S4_C PF16121 212 - 258 7.6e-26 40S ribosomal protein S4 C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 327
Acc65I GGTACC 1 cut(s) 692
AccB1I GGYRCC 1 cut(s) 692
AccB7I CCANNNNNTGG 2 cut(s) 51, 702
AccII CGCG 1 cut(s) 7
AclWI GGATC 1 cut(s) 446
AcoI YGGCCR 1 cut(s) 394
AcsI RAATTY 2 cut(s) 292, 650
AcuI CTGAAG 1 cut(s) 35
AcyI GRCGYC 1 cut(s) 636
AfaI GTAC 1 cut(s) 694
AfiI CCNNNNNNNGG 8 cut(s) 51, 98, 321, 429, 666, 702, 718, 762
AgeI ACCGGT 1 cut(s) 150
AgsI TTSAA 4 cut(s) 28, 157, 339, 364
AjiI CACGTC 1 cut(s) 628
AjnI CCWGG 1 cut(s) 630
AjuI GAANNNNNNNTTGG 2 cut(s) 308, 340
AluBI AGCT 5 cut(s) 484, 612, 736, 770, 785
AluI AGCT 5 cut(s) 484, 612, 736, 770, 785
Alw26I GTCTC 4 cut(s) 274, 305, 488, 611
AlwI GGATC 1 cut(s) 446
AoxI GGCC 2 cut(s) 394, 753
ApeKI GCWGC 3 cut(s) 770, 785, 788
ApoI RAATTY 2 cut(s) 292, 650
AseI ATTAAT 1 cut(s) 417
AsiGI ACCGGT 1 cut(s) 150
Asp700I GAANNNNTTC 1 cut(s) 674
Asp718I GGTACC 1 cut(s) 692
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 2 cut(s) 101, 376
AsuHPI GGTGA 1 cut(s) 559
AvaII GGWCC 2 cut(s) 101, 376
BalI TGGCCA 1 cut(s) 396
BanI GGYRCC 1 cut(s) 692
BbsI GAAGAC 1 cut(s) 116
BbvI GCAGC 2 cut(s) 757, 772
BccI CCATC 4 cut(s) 197, 212, 422, 485
BciT130I CCWGG 1 cut(s) 632
BclI TGATCA 1 cut(s) 132
BcoDI GTCTC 4 cut(s) 274, 305, 488, 611
BfaI CTAG 1 cut(s) 793
BfmI CTRYAG 1 cut(s) 786
BisI GCNGC 3 cut(s) 771, 786, 789
BlsI GCNGC 3 cut(s) 772, 787, 790
Bme1390I CCNGG 1 cut(s) 632
Bme18I GGWCC 2 cut(s) 101, 376
BmgBI CACGTC 1 cut(s) 628
BmgT120I GGNCC 2 cut(s) 101, 376
BmiI GGNNCC 2 cut(s) 326, 694
BmrFI CCNGG 1 cut(s) 632
BmsI GCATC 2 cut(s) 45, 186
BpiI GAAGAC 1 cut(s) 116
BsaBI GATNNNNATC 1 cut(s) 137
BsaHI GRCGYC 1 cut(s) 636
BsaI GGTCTC 1 cut(s) 488
BsaJI CCNNGG 2 cut(s) 701, 717
BsaWI WCCGGW 1 cut(s) 150
Bsc4I CCNNNNNNNGG 8 cut(s) 51, 98, 321, 429, 666, 702, 718, 762
Bse118I RCCGGY 1 cut(s) 150
Bse1I ACTGG 1 cut(s) 646
Bse3DI GCAATG 1 cut(s) 183
Bse8I GATNNNNATC 1 cut(s) 137
BseBI CCWGG 1 cut(s) 632
BseDI CCNNGG 2 cut(s) 701, 717
BseGI GGATG 3 cut(s) 60, 268, 352
BseJI GATNNNNATC 1 cut(s) 137
BseLI CCNNNNNNNGG 8 cut(s) 51, 98, 321, 429, 666, 702, 718, 762
BseMI GCAATG 1 cut(s) 183
BseNI ACTGG 1 cut(s) 646
BseRI GAGGAG 1 cut(s) 764
BseXI GCAGC 2 cut(s) 757, 772
BsgI GTGCAG 1 cut(s) 407
Bsh1236I CGCG 1 cut(s) 7
BshFI GGCC 2 cut(s) 396, 755
BshNI GGYRCC 1 cut(s) 692
BshTI ACCGGT 1 cut(s) 150
BsiSI CCGG 1 cut(s) 151
BslI CCNNNNNNNGG 8 cut(s) 51, 98, 321, 429, 666, 702, 718, 762
BsmAI GTCTC 4 cut(s) 274, 305, 488, 611
BsmBI CGTCTC 1 cut(s) 305
BsmI GAATGC 1 cut(s) 334
BsnI GGCC 2 cut(s) 396, 755
Bso31I GGTCTC 1 cut(s) 488
Bsp143I GATC 2 cut(s) 132, 451
Bsp19I CCATGG 1 cut(s) 701
BspANI GGCC 2 cut(s) 396, 755
BspFNI CGCG 1 cut(s) 7
BspLI GGNNCC 2 cut(s) 326, 694
BspMAI CTGCAG 1 cut(s) 790
BspPI GGATC 1 cut(s) 446
BspT107I GGYRCC 1 cut(s) 692
BspTNI GGTCTC 1 cut(s) 488
BsrDI GCAATG 1 cut(s) 183
BsrFI RCCGGY 1 cut(s) 150
BsrI ACTGG 1 cut(s) 646
BssAI RCCGGY 1 cut(s) 150
BssECI CCNNGG 2 cut(s) 701, 717
BssMI GATC 2 cut(s) 132, 451
BssNI GRCGYC 1 cut(s) 636
BssT1I CCWWGG 2 cut(s) 701, 717
Bst2UI CCWGG 1 cut(s) 632
Bst4CI ACNGT 2 cut(s) 176, 324
Bst6I CTCTTC 3 cut(s) 23, 345, 755
BstACI GRCGYC 1 cut(s) 636
BstAPI GCANNNNNTGC 1 cut(s) 55
BstC8I GCNNGC 1 cut(s) 768
BstDSI CCRYGG 1 cut(s) 701
BstENI CCTNNNNNAGG 1 cut(s) 716
BstF5I GGATG 3 cut(s) 60, 268, 352
BstFNI CGCG 1 cut(s) 7
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 2 cut(s) 135, 454
BstMAI GTCTC 4 cut(s) 274, 305, 488, 611
BstMBI GATC 2 cut(s) 132, 451
BstMWI GCNNNNNNNGC 3 cut(s) 55, 776, 785
BstNI CCWGG 1 cut(s) 632
BstSCI CCNGG 1 cut(s) 630
BstSFI CTRYAG 1 cut(s) 786
BstUI CGCG 1 cut(s) 7
BstV1I GCAGC 2 cut(s) 757, 772
BstV2I GAAGAC 1 cut(s) 116
BsuRI GGCC 2 cut(s) 396, 755
BtgI CCRYGG 1 cut(s) 701
BtrI CACGTC 1 cut(s) 628
BtsCI GGATG 3 cut(s) 60, 268, 352
BtsIMutI CAGTG 1 cut(s) 639
Cac8I GCNNGC 1 cut(s) 768
CfoI GCGC 1 cut(s) 7
Cfr10I RCCGGY 1 cut(s) 150
Cfr13I GGNCC 2 cut(s) 101, 376
CseI GACGC 1 cut(s) 644
Csp6I GTAC 1 cut(s) 693
CspAI ACCGGT 1 cut(s) 150
CspCI CAANNNNNGTGG 2 cut(s) 52, 87
CviAII CATG 5 cut(s) 206, 259, 544, 647, 702
CviQI GTAC 1 cut(s) 693
DpnI GATC 2 cut(s) 134, 453
DpnII GATC 2 cut(s) 132, 451
DrdI GACNNNNNNGTC 1 cut(s) 327
DseDI GACNNNNNNGTC 1 cut(s) 327
EaeI YGGCCR 1 cut(s) 394
Eam1104I CTCTTC 3 cut(s) 23, 345, 755
EarI CTCTTC 3 cut(s) 23, 345, 755
Eco130I CCWWGG 2 cut(s) 701, 717
Eco31I GGTCTC 1 cut(s) 488
Eco47I GGWCC 2 cut(s) 101, 376
Eco57I CTGAAG 1 cut(s) 35
EcoNI CCTNNNNNAGG 1 cut(s) 716
EcoRII CCWGG 1 cut(s) 630
EcoT14I CCWWGG 2 cut(s) 701, 717
ErhI CCWWGG 2 cut(s) 701, 717
Esp3I CGTCTC 1 cut(s) 305
FaeI CATG 5 cut(s) 209, 262, 547, 650, 705
FalI AAGNNNNNCTT 2 cut(s) 596, 628
FatI CATG 5 cut(s) 205, 258, 543, 646, 701
FbaI TGATCA 1 cut(s) 132
Fnu4HI GCNGC 3 cut(s) 771, 786, 789
FokI GGATG 3 cut(s) 67, 275, 359
Fsp4HI GCNGC 3 cut(s) 771, 786, 789
FspBI CTAG 1 cut(s) 793
GlaI GCGC 1 cut(s) 6
GluI GCNGC 3 cut(s) 771, 786, 789
HaeIII GGCC 2 cut(s) 396, 755
HapII CCGG 1 cut(s) 151
HgaI GACGC 1 cut(s) 644
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 636
Hin1II CATG 5 cut(s) 209, 262, 547, 650, 705
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 1 cut(s) 511
HindII GTYRAC 1 cut(s) 511
HindIII AAGCTT 3 cut(s) 482, 610, 734
HpaII CCGG 1 cut(s) 151
HphI GGTGA 1 cut(s) 559
Hpy166II GTNNAC 2 cut(s) 511, 678
Hpy188I TCNGA 1 cut(s) 168
Hpy188III TCNNGA 3 cut(s) 99, 193, 586
Hpy8I GTNNAC 2 cut(s) 511, 678
HpyAV CCTTC 2 cut(s) 258, 722
HpyCH4III ACNGT 2 cut(s) 176, 324
HpyCH4IV ACGT 2 cut(s) 202, 627
HpyCH4V TGCA 7 cut(s) 77, 199, 334, 372, 388, 656, 788
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 776, 785
HpySE526I ACGT 2 cut(s) 202, 627
Hsp92I GRCGYC 1 cut(s) 636
Hsp92II CATG 5 cut(s) 209, 262, 547, 650, 705
HspAI GCGC 1 cut(s) 5
KpnI GGTACC 1 cut(s) 696
Ksp22I TGATCA 1 cut(s) 132
Kzo9I GATC 2 cut(s) 132, 451
Lsp1109I GCAGC 2 cut(s) 757, 772
LweI GCATC 2 cut(s) 45, 186
MaeI CTAG 1 cut(s) 793
MaeII ACGT 2 cut(s) 202, 627
MaeIII GTNAC 2 cut(s) 443, 547
MalI GATC 2 cut(s) 134, 453
MboI GATC 2 cut(s) 132, 451
MboII GAAGA 5 cut(s) 28, 40, 116, 362, 772
MfeI CAATTG 1 cut(s) 681
MlsI TGGCCA 1 cut(s) 396
MluCI AATT 5 cut(s) 65, 292, 438, 650, 681
MluNI TGGCCA 1 cut(s) 396
MmeI TCCRAC 1 cut(s) 556
Mox20I TGGCCA 1 cut(s) 396
MroXI GAANNNNTTC 1 cut(s) 674
MscI TGGCCA 1 cut(s) 396
MseI TTAA 2 cut(s) 417, 732
MslI CAYNNNNRTG 1 cut(s) 194
Msp20I TGGCCA 1 cut(s) 396
MspA1I CMGCKG 1 cut(s) 785
MspI CCGG 1 cut(s) 151
MspR9I CCNGG 1 cut(s) 632
MunI CAATTG 1 cut(s) 681
Mva1269I GAATGC 1 cut(s) 334
MvaI CCWGG 1 cut(s) 632
MvnI CGCG 1 cut(s) 7
MwoI GCNNNNNNNGC 3 cut(s) 55, 776, 785
NcoI CCATGG 1 cut(s) 701
NdeII GATC 2 cut(s) 132, 451
NlaIII CATG 5 cut(s) 209, 262, 547, 650, 705
NlaIV GGNNCC 2 cut(s) 326, 694
NmuCI GTSAC 1 cut(s) 547
PctI GAATGC 1 cut(s) 334
PdmI GAANNNNTTC 1 cut(s) 674
PflMI CCANNNNNTGG 2 cut(s) 51, 702
PfoI TCCNGGA 1 cut(s) 630
PinAI ACCGGT 1 cut(s) 150
PkrI GCNGC 3 cut(s) 772, 787, 790
PshBI ATTAAT 1 cut(s) 417
Psp6I CCWGG 1 cut(s) 630
PspGI CCWGG 1 cut(s) 630
PspN4I GGNNCC 2 cut(s) 326, 694
PspPI GGNCC 2 cut(s) 101, 376
PstI CTGCAG 1 cut(s) 790
PvuII CAGCTG 1 cut(s) 785
RsaI GTAC 1 cut(s) 694
RsaNI GTAC 1 cut(s) 693
RseI CAYNNNNRTG 1 cut(s) 194
SaqAI TTAA 2 cut(s) 417, 732
SatI GCNGC 3 cut(s) 771, 786, 789
Sau3AI GATC 2 cut(s) 132, 451
Sau96I GGNCC 2 cut(s) 101, 376
ScrFI CCNGG 1 cut(s) 632
SfaNI GCATC 2 cut(s) 45, 186
SfcI CTRYAG 1 cut(s) 786
SinI GGWCC 2 cut(s) 101, 376
SmiMI CAYNNNNRTG 1 cut(s) 194
Sse9I AATT 5 cut(s) 65, 292, 438, 650, 681
SspMI CTAG 1 cut(s) 793
StyD4I CCNGG 1 cut(s) 630
StyI CCWWGG 2 cut(s) 701, 717
TaaI ACNGT 2 cut(s) 176, 324
TaiI ACGT 2 cut(s) 205, 630
TaqI TCGA 2 cut(s) 113, 525
TasI AATT 5 cut(s) 65, 292, 438, 650, 681
Tru1I TTAA 2 cut(s) 417, 732
Tru9I TTAA 2 cut(s) 417, 732
TscAI CASTG 1 cut(s) 646
TseFI GTSAC 1 cut(s) 547
TseI GCWGC 3 cut(s) 770, 785, 788
Tsp45I GTSAC 1 cut(s) 547
TspDTI ATGAA 4 cut(s) 247, 363, 505, 663
TspGWI ACGGA 3 cut(s) 287, 317, 368
TspRI CASTG 1 cut(s) 646
Van91I CCANNNNNTGG 2 cut(s) 51, 702
VpaK11BI GGWCC 2 cut(s) 101, 376
VspI ATTAAT 1 cut(s) 417
XagI CCTNNNNNAGG 1 cut(s) 716
XapI RAATTY 2 cut(s) 292, 650
XcmI CCANNNNNNNNNTGG 1 cut(s) 638
XmnI GAANNNNTTC 1 cut(s) 674
XspI CTAG 1 cut(s) 793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.