MD05G1242900.v1.1

Belongs to the eukaryotic ribosomal protein eS4 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001275
Physical Location & Seq
Forward (+)
37572485 .. 37574699
2215 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1242900.v1.1.491

Sequence Viewer

Length: 795 bp
ATGGCTCGTGGTTTGAAGAAACATTTGAAGAGGCTCAATGCCCCCAAGCATTGGATGCTCGACAAACTTGGCGGTGCATTTGCTCCCAAGCCTTCGTCTGGACCTCACAAATCCAGGGAGTGCCTGCCATTGGTTATCATCTTGCGTAACCGATTGAAGTATGCTCTGACCTACCGTGAGGTCATTTCCATTCTGATGCAACGACATGTTCTCGTTGATGGGAAAGTGAGGACAGATAAGACATATCCGTCTGGTTTCATGGATGTTGTTTCAATCCCCAAGACAAATGAGAACTTCCGTCTCCTTTATGACACCAAGGGTCGGTTCCGTCTCCACTCCATCAGGGATGAAGAAGCAAAGTTTAAACTCTGCAAAGTCCGCTCTGTGCAGTTTGGACAGAAAGGCATTCCATATATTAACACCTATGATGGGCGAACAATCCGTTACCCAGACCCTCTCATCAAGGCAAATGACACCATTAAGTTGGACTTGGAGACCAACAAGATCACTGACTTCATCAAGTTTGATGTCGGCAATGTGGTCATGGTGACTGGAGGAAGGAACAGAGGGCGTGTTGGAGTAATCAAGAACAGGGAGAAGCACAAGGGAAGCTTCGAGACTATCCACGTCCAGGATGCCAGTGGTCATGAGTTTGCAACCCGGTTGGGCAATGTGTTCACAATTGGCAAGGGGACCAAGCCATGGATAAGCCTTCCCAAGGGTAAGGGTATTAAGCTTACCATTATTGAAGAGGCCAAGAAGAGAGCAGCAGCGCAAGCCACAGCCACTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
40S_S4_C KOW RS4NT Ribosomal_S4e S4

Protein Analysis

265

Amino Acids

29.82

Weight (kDa)

10.25

Isoelectric Point (pI)

20.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RS4NT PF08071 3 - 39 2.3e-18 RS4NT (NUC023) domain
Ribosomal_S4e PF00900 95 - 169 1.2e-34 Ribosomal family S4e
KOW PF00467 177 - 210 1.9e-06 KOW motif
40S_S4_C PF16121 212 - 258 1.3e-25 40S ribosomal protein S4 C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 247
AccB7I CCANNNNNTGG 2 cut(s) 51, 702
AccBSI CCGCTC 1 cut(s) 381
AciI CCGC 2 cut(s) 72, 379
AfiI CCNNNNNNNGG 8 cut(s) 51, 98, 130, 321, 429, 631, 702, 718
AflIII ACRYGT 1 cut(s) 205
AgsI TTSAA 5 cut(s) 16, 28, 157, 273, 749
AjiI CACGTC 1 cut(s) 628
AjnI CCWGG 2 cut(s) 113, 630
AjuI GAANNNNNNNTTGG 2 cut(s) 308, 340
AluBI AGCT 2 cut(s) 612, 736
AluI AGCT 2 cut(s) 612, 736
Alw26I GTCTC 4 cut(s) 305, 335, 488, 611
AlwNI CAGNNNCTG 1 cut(s) 788
AoxI GGCC 1 cut(s) 753
ApeKI GCWGC 2 cut(s) 767, 770
AspLEI GCGC 1 cut(s) 775
AspS9I GGNCC 2 cut(s) 101, 693
AsuC2I CCSGG 1 cut(s) 661
AsuHPI GGTGA 1 cut(s) 559
AvaII GGWCC 2 cut(s) 101, 693
BauI CACGAG 1 cut(s) 6
BbvI GCAGC 2 cut(s) 779, 782
BccI CCATC 3 cut(s) 212, 347, 422
BciT130I CCWGG 2 cut(s) 115, 632
BcnI CCSGG 1 cut(s) 661
BcoDI GTCTC 4 cut(s) 305, 335, 488, 611
BisI GCNGC 2 cut(s) 768, 771
BlsI GCNGC 2 cut(s) 769, 772
Bme1390I CCNGG 3 cut(s) 115, 632, 661
Bme18I GGWCC 2 cut(s) 101, 693
BmgBI CACGTC 1 cut(s) 628
BmgT120I GGNCC 2 cut(s) 101, 693
BmiI GGNNCC 2 cut(s) 326, 694
BmrFI CCNGG 3 cut(s) 115, 632, 661
BmsI GCATC 3 cut(s) 45, 186, 625
BpmI CTGGAG 1 cut(s) 573
BpuMI CCSGG 1 cut(s) 661
BsaI GGTCTC 1 cut(s) 488
BsaJI CCNNGG 4 cut(s) 114, 315, 701, 717
Bsc4I CCNNNNNNNGG 8 cut(s) 51, 98, 130, 321, 429, 631, 702, 718
Bse1I ACTGG 2 cut(s) 556, 639
Bse3DI GCAATG 2 cut(s) 541, 676
BseBI CCWGG 2 cut(s) 115, 632
BseDI CCNNGG 4 cut(s) 114, 315, 701, 717
BseGI GGATG 4 cut(s) 60, 268, 352, 640
BseLI CCNNNNNNNGG 8 cut(s) 51, 98, 130, 321, 429, 631, 702, 718
BseMI GCAATG 2 cut(s) 541, 676
BseNI ACTGG 2 cut(s) 556, 639
BseXI GCAGC 2 cut(s) 779, 782
BsgI GTGCAG 1 cut(s) 407
BshFI GGCC 1 cut(s) 755
BsiSI CCGG 1 cut(s) 661
BslFI GGGAC 1 cut(s) 706
BslI CCNNNNNNNGG 8 cut(s) 51, 98, 130, 321, 429, 631, 702, 718
BsmAI GTCTC 4 cut(s) 305, 335, 488, 611
BsmBI CGTCTC 2 cut(s) 305, 335
BsmFI GGGAC 1 cut(s) 706
BsmI GAATGC 1 cut(s) 405
BsnI GGCC 1 cut(s) 755
Bso31I GGTCTC 1 cut(s) 488
Bsp143I GATC 1 cut(s) 504
Bsp19I CCATGG 1 cut(s) 701
BspACI CCGC 2 cut(s) 72, 379
BspANI GGCC 1 cut(s) 755
BspHI TCATGA 1 cut(s) 646
BspLI GGNNCC 2 cut(s) 326, 694
BspTNI GGTCTC 1 cut(s) 488
BsrBI CCGCTC 1 cut(s) 381
BsrDI GCAATG 2 cut(s) 541, 676
BsrI ACTGG 2 cut(s) 556, 639
BssECI CCNNGG 4 cut(s) 114, 315, 701, 717
BssMI GATC 1 cut(s) 504
BssSI CACGAG 1 cut(s) 6
BssT1I CCWWGG 3 cut(s) 315, 701, 717
Bst2BI CACGAG 1 cut(s) 6
Bst2UI CCWGG 2 cut(s) 115, 632
Bst4CI ACNGT 1 cut(s) 176
Bst6I CTCTTC 3 cut(s) 23, 744, 755
BstAPI GCANNNNNTGC 1 cut(s) 55
BstC8I GCNNGC 2 cut(s) 125, 777
BstDSI CCRYGG 1 cut(s) 701
BstENI CCTNNNNNAGG 1 cut(s) 716
BstF5I GGATG 4 cut(s) 60, 268, 352, 640
BstHHI GCGC 1 cut(s) 775
BstKTI GATC 1 cut(s) 507
BstMAI GTCTC 4 cut(s) 305, 335, 488, 611
BstMBI GATC 1 cut(s) 504
BstMWI GCNNNNNNNGC 3 cut(s) 55, 378, 776
BstNI CCWGG 2 cut(s) 115, 632
BstNSI RCATGY 1 cut(s) 209
BstSCI CCNGG 3 cut(s) 113, 630, 659
BstV1I GCAGC 2 cut(s) 779, 782
BstXI CCANNNNNNTGG 1 cut(s) 484
BsuRI GGCC 1 cut(s) 755
BtgI CCRYGG 1 cut(s) 701
BtrI CACGTC 1 cut(s) 628
BtsCI GGATG 4 cut(s) 60, 268, 352, 640
BtsI GCAGTG 1 cut(s) 786
BtsIMutI CAGTG 3 cut(s) 507, 646, 786
Cac8I GCNNGC 2 cut(s) 125, 777
CaiI CAGNNNCTG 1 cut(s) 788
CciI TCATGA 1 cut(s) 646
CfoI GCGC 1 cut(s) 775
Cfr13I GGNCC 2 cut(s) 101, 693
CviAII CATG 5 cut(s) 206, 259, 544, 647, 702
DpnI GATC 1 cut(s) 506
DpnII GATC 1 cut(s) 504
DraI TTTAAA 1 cut(s) 364
DrdI GACNNNNNNGTC 1 cut(s) 247
DseDI GACNNNNNNGTC 1 cut(s) 247
Eam1104I CTCTTC 3 cut(s) 23, 744, 755
EarI CTCTTC 3 cut(s) 23, 744, 755
Eco130I CCWWGG 3 cut(s) 315, 701, 717
Eco31I GGTCTC 1 cut(s) 488
Eco47I GGWCC 2 cut(s) 101, 693
EcoNI CCTNNNNNAGG 1 cut(s) 716
EcoRII CCWGG 2 cut(s) 113, 630
EcoT14I CCWWGG 3 cut(s) 315, 701, 717
ErhI CCWWGG 3 cut(s) 315, 701, 717
Esp3I CGTCTC 2 cut(s) 305, 335
FaeI CATG 5 cut(s) 209, 262, 547, 650, 705
FalI AAGNNNNNCTT 4 cut(s) 473, 505, 596, 628
FaqI GGGAC 1 cut(s) 706
FatI CATG 5 cut(s) 205, 258, 543, 646, 701
Fnu4HI GCNGC 2 cut(s) 768, 771
FokI GGATG 4 cut(s) 67, 275, 359, 647
Fsp4HI GCNGC 2 cut(s) 768, 771
GlaI GCGC 1 cut(s) 774
GluI GCNGC 2 cut(s) 768, 771
GsuI CTGGAG 1 cut(s) 573
HaeIII GGCC 1 cut(s) 755
HapII CCGG 1 cut(s) 661
HhaI GCGC 1 cut(s) 775
Hin1II CATG 5 cut(s) 209, 262, 547, 650, 705
Hin6I GCGC 1 cut(s) 773
HinP1I GCGC 1 cut(s) 773
HindIII AAGCTT 2 cut(s) 610, 734
HpaII CCGG 1 cut(s) 661
HphI GGTGA 1 cut(s) 559
Hpy166II GTNNAC 1 cut(s) 678
Hpy188I TCNGA 2 cut(s) 168, 195
Hpy188III TCNNGA 4 cut(s) 99, 586, 616, 647
Hpy8I GTNNAC 1 cut(s) 678
HpyAV CCTTC 3 cut(s) 102, 552, 722
HpyCH4III ACNGT 1 cut(s) 176
HpyCH4IV ACGT 1 cut(s) 627
HpyCH4V TGCA 5 cut(s) 77, 199, 372, 388, 656
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 378, 776
HpySE526I ACGT 1 cut(s) 627
Hsp92II CATG 5 cut(s) 209, 262, 547, 650, 705
HspAI GCGC 1 cut(s) 773
Kzo9I GATC 1 cut(s) 504
LmnI GCTCC 1 cut(s) 88
Lsp1109I GCAGC 2 cut(s) 779, 782
LweI GCATC 3 cut(s) 45, 186, 625
MaeII ACGT 1 cut(s) 627
MaeIII GTNAC 3 cut(s) 146, 443, 547
MalI GATC 1 cut(s) 506
MbiI CCGCTC 1 cut(s) 381
MboI GATC 1 cut(s) 504
MboII GAAGA 5 cut(s) 28, 40, 362, 761, 772
MfeI CAATTG 1 cut(s) 681
MluCI AATT 1 cut(s) 681
MmeI TCCRAC 2 cut(s) 465, 556
MnlI CCTC 8 cut(s) 24, 114, 172, 222, 465, 548, 560, 745
MseI TTAA 5 cut(s) 363, 417, 480, 732, 793
MslI CAYNNNNRTG 1 cut(s) 194
MspI CCGG 1 cut(s) 661
MspR9I CCNGG 3 cut(s) 115, 632, 661
MssI GTTTAAAC 1 cut(s) 364
MunI CAATTG 1 cut(s) 681
Mva1269I GAATGC 1 cut(s) 405
MvaI CCWGG 2 cut(s) 115, 632
MwoI GCNNNNNNNGC 3 cut(s) 55, 378, 776
NciI CCSGG 1 cut(s) 661
NcoI CCATGG 1 cut(s) 701
NdeII GATC 1 cut(s) 504
NlaIII CATG 5 cut(s) 209, 262, 547, 650, 705
NlaIV GGNNCC 2 cut(s) 326, 694
NmuCI GTSAC 1 cut(s) 547
NspI RCATGY 1 cut(s) 209
PagI TCATGA 1 cut(s) 646
PciI ACATGT 1 cut(s) 205
PctI GAATGC 1 cut(s) 405
PflMI CCANNNNNTGG 2 cut(s) 51, 702
PfoI TCCNGGA 1 cut(s) 630
PkrI GCNGC 2 cut(s) 769, 772
PmeI GTTTAAAC 1 cut(s) 364
PscI ACATGT 1 cut(s) 205
Psp6I CCWGG 2 cut(s) 113, 630
PspGI CCWGG 2 cut(s) 113, 630
PspN4I GGNNCC 2 cut(s) 326, 694
PspPI GGNCC 2 cut(s) 101, 693
PstNI CAGNNNCTG 1 cut(s) 788
RseI CAYNNNNRTG 1 cut(s) 194
SaqAI TTAA 5 cut(s) 363, 417, 480, 732, 793
SatI GCNGC 2 cut(s) 768, 771
Sau3AI GATC 1 cut(s) 504
Sau96I GGNCC 2 cut(s) 101, 693
ScrFI CCNGG 3 cut(s) 115, 632, 661
SetI ASST 7 cut(s) 106, 173, 183, 425, 614, 630, 738
SfaNI GCATC 3 cut(s) 45, 186, 625
SinI GGWCC 2 cut(s) 101, 693
SmiMI CAYNNNNRTG 1 cut(s) 194
Sse9I AATT 1 cut(s) 681
SsiI CCGC 2 cut(s) 72, 379
StyD4I CCNGG 3 cut(s) 113, 630, 659
StyI CCWWGG 3 cut(s) 315, 701, 717
TaaI ACNGT 1 cut(s) 176
TaiI ACGT 1 cut(s) 630
TaqI TCGA 2 cut(s) 60, 615
TasI AATT 1 cut(s) 681
Tru1I TTAA 5 cut(s) 363, 417, 480, 732, 793
Tru9I TTAA 5 cut(s) 363, 417, 480, 732, 793
TscAI CASTG 3 cut(s) 514, 646, 793
TseFI GTSAC 1 cut(s) 547
TseI GCWGC 2 cut(s) 767, 770
Tsp45I GTSAC 1 cut(s) 547
TspDTI ATGAA 3 cut(s) 247, 363, 505
TspGWI ACGGA 4 cut(s) 237, 287, 317, 431
TspRI CASTG 3 cut(s) 514, 646, 793
Van91I CCANNNNNTGG 2 cut(s) 51, 702
VpaK11BI GGWCC 2 cut(s) 101, 693
XagI CCTNNNNNAGG 1 cut(s) 716
XceI RCATGY 1 cut(s) 209
XcmI CCANNNNNNNNNTGG 1 cut(s) 638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.