Rh4AG270900

Belongs to the eukaryotic ribosomal protein eS4 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0001275
Physical Location & Seq
Forward (+)
58699319 .. 58700401
1083 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG270900.1

Sequence Viewer

Length: 795 bp
ATGGCGAGAGGTTTGAAGAAGCATCTGAAGAGGCTCAATGCCCCAAAGCATTGGATGCTCGACAAGCTTGGTGGTGCTTTTGCTCCCAAACCATCATCTGGGCCACACAAATCGAGGGAGTGCCTTCCCCTGATCATCATTCTGAGGAACAGGTTGAAGTATGCTCTCACATACCGTGAGGTCATTGCTATTCTCATGCAACGTCATGTTTTGGTTGATGGAAAGGTCAGGACTGATAAGACCTACCCTGCTGGCTTCATGGATGTTGTTTCCATCCCAAAAACCAATGAGAACTTCCGTCTGCTCTACGACACCAAGGGTCGTTTCCGTCTCCATTCAATCAGGGATGAGGAGGCAAAGTTCAAGCTCTGCAAGGTTCGCTCAGTGCAGTTTGGGCAGAAGAACATCCCCTATATTAACACTTATGATGGGAGAACTATTCGCTACCCAGACCCACTTATTAAGGCCAATGATACTATCAAGCTGGACTTAGAGACCAACAAGATTATTGACTTCATTAAGTTTGATGTTGGGAATGTTGTCATGGTGACTGGTGGAAGGAATAGGGGACGTGTTGGAGTCATTAAGAACAGGGAAAAGTATAAGGGAAGCTTTGAGACCATCCACGTTCAGGATGCCGCAGGTCATGAGTTTGCCACCCGTCTTGGCAATGTGTTCACCATTGGCAAGGGTACAAAGCCTTGGGTGTCACTTCCCAAGGGCAAGGGTATCAAGCTCTCCATCATTGAGGAGGCAAGGAAGCGACAAGCAGCACTCCAAACTGCTACTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
40S_S4_C KOW RS4NT Ribosomal_S4e S4

Protein Analysis

264

Amino Acids

29.98

Weight (kDa)

10.25

Isoelectric Point (pI)

24.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RS4NT PF08071 3 - 39 2.3e-18 RS4NT (NUC023) domain
S4 PF01479 44 - 90 4.3e-06 S4 domain
Ribosomal_S4e PF00900 95 - 169 4.4e-34 Ribosomal family S4e
40S_S4_C PF16121 212 - 257 2.3e-25 40S ribosomal protein S4 C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 632
AccB7I CCANNNNNTGG 1 cut(s) 98
AciI CCGC 1 cut(s) 639
AcuI CTGAAG 1 cut(s) 47
AfaI GTAC 1 cut(s) 694
AfiI CCNNNNNNNGG 2 cut(s) 98, 631
AflIII ACRYGT 1 cut(s) 571
AgsI TTSAA 4 cut(s) 16, 157, 339, 364
AjiI CACGTC 1 cut(s) 572
AjuI GAANNNNNNNTTGG 4 cut(s) 278, 308, 310, 340
AloI GAACNNNNNNTCC 2 cut(s) 344, 376
AluBI AGCT 5 cut(s) 67, 367, 484, 612, 736
AluI AGCT 5 cut(s) 67, 367, 484, 612, 736
Alw26I GTCTC 3 cut(s) 335, 488, 611
AoxI GGCC 2 cut(s) 101, 465
ApeKI GCWGC 1 cut(s) 770
AspS9I GGNCC 1 cut(s) 101
AsuHPI GGTGA 2 cut(s) 559, 670
BbvI GCAGC 1 cut(s) 782
BccI CCATC 6 cut(s) 100, 212, 281, 422, 629, 749
BclI TGATCA 1 cut(s) 132
BcoDI GTCTC 3 cut(s) 335, 488, 611
BfuAI ACCTGC 1 cut(s) 632
BisI GCNGC 2 cut(s) 639, 771
BlsI GCNGC 2 cut(s) 640, 772
BmgBI CACGTC 1 cut(s) 572
BmgT120I GGNCC 1 cut(s) 101
BmsI GCATC 3 cut(s) 31, 45, 625
BsaI GGTCTC 2 cut(s) 488, 611
BsaJI CCNNGG 3 cut(s) 315, 701, 717
BsaXI ACNNNNNCTCC 2 cut(s) 344, 374
Bsc4I CCNNNNNNNGG 2 cut(s) 98, 631
Bse1I ACTGG 1 cut(s) 556
Bse3DI GCAATG 2 cut(s) 183, 676
BseDI CCNNGG 3 cut(s) 315, 701, 717
BseGI GGATG 7 cut(s) 60, 268, 273, 352, 405, 621, 640
BseLI CCNNNNNNNGG 2 cut(s) 98, 631
BseMI GCAATG 2 cut(s) 183, 676
BseMII CTCAG 2 cut(s) 134, 396
BseNI ACTGG 1 cut(s) 556
BseRI GAGGAG 2 cut(s) 365, 764
BseXI GCAGC 1 cut(s) 782
BsgI GTGCAG 1 cut(s) 407
BshFI GGCC 2 cut(s) 103, 467
BslFI GGGAC 1 cut(s) 582
BslI CCNNNNNNNGG 2 cut(s) 98, 631
BsmAI GTCTC 3 cut(s) 335, 488, 611
BsmBI CGTCTC 1 cut(s) 335
BsmFI GGGAC 1 cut(s) 582
BsnI GGCC 2 cut(s) 103, 467
Bso31I GGTCTC 2 cut(s) 488, 611
Bsp143I GATC 1 cut(s) 132
BspACI CCGC 1 cut(s) 639
BspANI GGCC 2 cut(s) 103, 467
BspCNI CTCAG 2 cut(s) 135, 395
BspHI TCATGA 1 cut(s) 646
BspMI ACCTGC 1 cut(s) 632
BspTNI GGTCTC 2 cut(s) 488, 611
BsrDI GCAATG 2 cut(s) 183, 676
BsrI ACTGG 1 cut(s) 556
BssECI CCNNGG 3 cut(s) 315, 701, 717
BssMI GATC 1 cut(s) 132
BssT1I CCWWGG 3 cut(s) 315, 701, 717
Bst4CI ACNGT 1 cut(s) 176
Bst6I CTCTTC 1 cut(s) 23
BstAPI GCANNNNNTGC 1 cut(s) 55
BstC8I GCNNGC 1 cut(s) 253
BstDEI CTNAG 3 cut(s) 143, 382, 490
BstF5I GGATG 7 cut(s) 60, 268, 273, 352, 405, 621, 640
BstKTI GATC 1 cut(s) 135
BstMAI GTCTC 3 cut(s) 335, 488, 611
BstMBI GATC 1 cut(s) 132
BstMWI GCNNNNNNNGC 4 cut(s) 55, 64, 378, 394
BstV1I GCAGC 1 cut(s) 782
BstXI CCANNNNNNTGG 1 cut(s) 51
BsuRI GGCC 2 cut(s) 103, 467
BtrI CACGTC 1 cut(s) 572
BtsCI GGATG 7 cut(s) 60, 268, 273, 352, 405, 621, 640
BtsIMutI CAGTG 1 cut(s) 390
BveI ACCTGC 1 cut(s) 632
Cac8I GCNNGC 1 cut(s) 253
CciI TCATGA 1 cut(s) 646
Cfr13I GGNCC 1 cut(s) 101
Csp6I GTAC 1 cut(s) 693
CspCI CAANNNNNGTGG 4 cut(s) 52, 87, 646, 681
CviAII CATG 5 cut(s) 196, 206, 259, 544, 647
CviQI GTAC 1 cut(s) 693
DdeI CTNAG 3 cut(s) 143, 382, 490
DpnI GATC 1 cut(s) 134
DpnII GATC 1 cut(s) 132
Eam1104I CTCTTC 1 cut(s) 23
EarI CTCTTC 1 cut(s) 23
Eco130I CCWWGG 3 cut(s) 315, 701, 717
Eco31I GGTCTC 2 cut(s) 488, 611
Eco57I CTGAAG 1 cut(s) 47
EcoT14I CCWWGG 3 cut(s) 315, 701, 717
ErhI CCWWGG 3 cut(s) 315, 701, 717
Esp3I CGTCTC 1 cut(s) 335
FaeI CATG 5 cut(s) 199, 209, 262, 547, 650
FalI AAGNNNNNCTT 4 cut(s) 473, 505, 596, 628
FaqI GGGAC 1 cut(s) 582
FatI CATG 5 cut(s) 195, 205, 258, 543, 646
FbaI TGATCA 1 cut(s) 132
Fnu4HI GCNGC 2 cut(s) 639, 771
FokI GGATG 7 cut(s) 67, 260, 275, 359, 392, 608, 647
Fsp4HI GCNGC 2 cut(s) 639, 771
GluI GCNGC 2 cut(s) 639, 771
HaeIII GGCC 2 cut(s) 103, 467
Hin1II CATG 5 cut(s) 199, 209, 262, 547, 650
HindIII AAGCTT 2 cut(s) 65, 610
HinfI GANTC 1 cut(s) 579
HphI GGTGA 2 cut(s) 559, 670
Hpy166II GTNNAC 1 cut(s) 678
Hpy188I TCNGA 2 cut(s) 27, 144
Hpy188III TCNNGA 3 cut(s) 229, 632, 647
Hpy8I GTNNAC 1 cut(s) 678
HpyAV CCTTC 2 cut(s) 134, 552
HpyCH4III ACNGT 1 cut(s) 176
HpyCH4IV ACGT 3 cut(s) 202, 571, 627
HpyCH4V TGCA 3 cut(s) 199, 372, 388
HpyF10VI GCNNNNNNNGC 4 cut(s) 55, 64, 378, 394
HpyF3I CTNAG 3 cut(s) 143, 382, 490
HpySE526I ACGT 3 cut(s) 202, 571, 627
Hsp92II CATG 5 cut(s) 199, 209, 262, 547, 650
Ksp22I TGATCA 1 cut(s) 132
Kzo9I GATC 1 cut(s) 132
LmnI GCTCC 1 cut(s) 88
Lsp1109I GCAGC 1 cut(s) 782
LweI GCATC 3 cut(s) 31, 45, 625
MaeII ACGT 3 cut(s) 202, 571, 627
MaeIII GTNAC 2 cut(s) 547, 708
MalI GATC 1 cut(s) 134
MboI GATC 1 cut(s) 132
MboII GAAGA 3 cut(s) 28, 40, 412
MlyI GAGTC 1 cut(s) 588
MmeI TCCRAC 1 cut(s) 556
MnlI CCTC 8 cut(s) 24, 108, 138, 172, 343, 346, 742, 745
MseI TTAA 5 cut(s) 417, 462, 519, 585, 793
MwoI GCNNNNNNNGC 4 cut(s) 55, 64, 378, 394
NdeII GATC 1 cut(s) 132
NlaIII CATG 5 cut(s) 199, 209, 262, 547, 650
NmuCI GTSAC 2 cut(s) 547, 708
PagI TCATGA 1 cut(s) 646
PflMI CCANNNNNTGG 1 cut(s) 98
PkrI GCNGC 2 cut(s) 640, 772
PleI GAGTC 1 cut(s) 587
PpsI GAGTC 1 cut(s) 587
PspPI GGNCC 1 cut(s) 101
RsaI GTAC 1 cut(s) 694
RsaNI GTAC 1 cut(s) 693
SaqAI TTAA 5 cut(s) 417, 462, 519, 585, 793
SatI GCNGC 2 cut(s) 639, 771
Sau3AI GATC 1 cut(s) 132
Sau96I GGNCC 1 cut(s) 101
SchI GAGTC 1 cut(s) 588
SfaNI GCATC 3 cut(s) 31, 45, 625
SsiI CCGC 1 cut(s) 639
StyI CCWWGG 3 cut(s) 315, 701, 717
TaaI ACNGT 1 cut(s) 176
TaiI ACGT 3 cut(s) 205, 574, 630
TaqI TCGA 2 cut(s) 60, 113
TauI GCSGC 1 cut(s) 641
Tru1I TTAA 5 cut(s) 417, 462, 519, 585, 793
Tru9I TTAA 5 cut(s) 417, 462, 519, 585, 793
TscAI CASTG 1 cut(s) 390
TseFI GTSAC 2 cut(s) 547, 708
TseI GCWGC 1 cut(s) 770
Tsp45I GTSAC 2 cut(s) 547, 708
TspDTI ATGAA 2 cut(s) 247, 505
TspGWI ACGGA 2 cut(s) 287, 317
TspRI CASTG 1 cut(s) 390
Van91I CCANNNNNTGG 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.