RchiOBHm_Chr5g0021781

Belongs to the eukaryotic ribosomal protein eS4 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001275
Physical Location & Seq
Reverse (-)
15940220 .. 15942906
2687 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30181

Sequence Viewer

Length: 795 bp
ATGGCGCGAGGACTGAAGAAGCATTTGAAGAGGCTCAATGCCCCCAAGCATTGGATGCTTGACAAATTGGGTGGTGCATTTGCCCCCAAGCCCTCATCTGGACCTCACAAATCCAGGGAATGTCTTCCGTTGATCATTATCTTGCGAAACCGGTTGAAGTATGCTTTGACATACCGTGAGGTTATTGCCATTTTGATGCAACGTCATGTTATGGTTGATGGGAAAGTTAGGACTGATAAGACTTATCCTTCTGGTTTCATGGATGTTGTCTCAATCCCCAAGACAAATGAGAATTTCCGTCTCCTTTATGACACCAAAGGACGGTTCCGTCTGCATTCAATCAGGGATGAAGAGGCAAAGTTCAAACTCTGCAAGGTCCGTTCTGTGCAGTTCGGCCAAAAGAACATCCCTTACATTAATACCTATGATGGAAGAACAATTCGTTACCCTGACCCTCTTATCAAGGCAAATGACACCATCAAGCTGGATTTGGAGACCAACAAGATCGTCGACTTTATCAAGTTTGATGTTGGTAATGTTGTCATGGTGACAGGTGGTAGGAACAGAGGGCGAGTTGGAGTAATCAAGAACAGGGAGAAGCATAAGGGAAGCTTTGAGACTATCCATGTCCAGGACGCCACTGGACATGAATTTGCAACCCGTTTGGCGAATGTGTTCACTATTGGTAAGGGGACCAAGCCATGGGTAAGCCTTCCCAAGGGTAAGGGTATTAAGCTTACCATCATTGAGGAGGCTAAGAAGAGGCAAGCAGCCCAAGCCACAGCTGCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
40S_S4_C KOW RS4NT Ribosomal_S4e S4

Protein Analysis

264

Amino Acids

29.93

Weight (kDa)

10.25

Isoelectric Point (pI)

22.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RS4NT PF08071 3 - 39 2.3e-18 RS4NT (NUC023) domain
Ribosomal_S4e PF00900 95 - 169 4.4e-34 Ribosomal family S4e
KOW PF00467 177 - 210 1.9e-06 KOW motif
40S_S4_C PF16121 212 - 258 6.8e-26 40S ribosomal protein S4 C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 327
AccB7I CCANNNNNTGG 2 cut(s) 51, 702
AccI GTMKAC 1 cut(s) 510
AccII CGCG 1 cut(s) 7
AcoI YGGCCR 1 cut(s) 394
AcsI RAATTY 2 cut(s) 292, 650
AcuI CTGAAG 1 cut(s) 35
AcyI GRCGYC 1 cut(s) 636
AfiI CCNNNNNNNGG 6 cut(s) 51, 98, 321, 631, 702, 718
AgeI ACCGGT 1 cut(s) 150
AgsI TTSAA 4 cut(s) 28, 157, 339, 364
AjnI CCWGG 2 cut(s) 113, 630
AjuI GAANNNNNNNTTGG 2 cut(s) 308, 340
AluBI AGCT 4 cut(s) 484, 612, 736, 785
AluI AGCT 4 cut(s) 484, 612, 736, 785
Alw26I GTCTC 4 cut(s) 274, 305, 488, 611
AlwNI CAGNNNCTG 1 cut(s) 788
AoxI GGCC 1 cut(s) 394
ApeKI GCWGC 3 cut(s) 770, 785, 788
ApoI RAATTY 2 cut(s) 292, 650
AseI ATTAAT 1 cut(s) 417
AsiGI ACCGGT 1 cut(s) 150
Asp700I GAANNNNTTC 2 cut(s) 123, 674
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 3 cut(s) 101, 376, 693
AsuHPI GGTGA 1 cut(s) 559
AvaII GGWCC 3 cut(s) 101, 376, 693
BbsI GAAGAC 1 cut(s) 116
BbvI GCAGC 3 cut(s) 772, 775, 782
BccI CCATC 4 cut(s) 212, 422, 485, 749
BciT130I CCWGG 2 cut(s) 115, 632
BclI TGATCA 1 cut(s) 132
BcoDI GTCTC 4 cut(s) 274, 305, 488, 611
BisI GCNGC 3 cut(s) 771, 786, 789
BlsI GCNGC 3 cut(s) 772, 787, 790
Bme1390I CCNGG 2 cut(s) 115, 632
Bme18I GGWCC 3 cut(s) 101, 376, 693
BmgT120I GGNCC 3 cut(s) 101, 376, 693
BmiI GGNNCC 2 cut(s) 326, 694
BmrFI CCNGG 2 cut(s) 115, 632
BmsI GCATC 2 cut(s) 45, 186
BpiI GAAGAC 1 cut(s) 116
BsaBI GATNNNNATC 1 cut(s) 137
BsaHI GRCGYC 1 cut(s) 636
BsaI GGTCTC 1 cut(s) 488
BsaJI CCNNGG 3 cut(s) 114, 701, 717
BsaWI WCCGGW 1 cut(s) 150
Bsc4I CCNNNNNNNGG 6 cut(s) 51, 98, 321, 631, 702, 718
Bse118I RCCGGY 1 cut(s) 150
Bse1I ACTGG 1 cut(s) 646
Bse8I GATNNNNATC 1 cut(s) 137
BseBI CCWGG 2 cut(s) 115, 632
BseDI CCNNGG 3 cut(s) 114, 701, 717
BseGI GGATG 4 cut(s) 60, 268, 352, 405
BseJI GATNNNNATC 1 cut(s) 137
BseLI CCNNNNNNNGG 6 cut(s) 51, 98, 321, 631, 702, 718
BseNI ACTGG 1 cut(s) 646
BseRI GAGGAG 1 cut(s) 764
BseXI GCAGC 3 cut(s) 772, 775, 782
BsgI GTGCAG 1 cut(s) 407
Bsh1236I CGCG 1 cut(s) 7
BshFI GGCC 1 cut(s) 396
BshTI ACCGGT 1 cut(s) 150
BsiSI CCGG 1 cut(s) 151
BslFI GGGAC 1 cut(s) 706
BslI CCNNNNNNNGG 6 cut(s) 51, 98, 321, 631, 702, 718
BsmAI GTCTC 4 cut(s) 274, 305, 488, 611
BsmBI CGTCTC 1 cut(s) 305
BsmFI GGGAC 1 cut(s) 706
BsmI GAATGC 1 cut(s) 334
BsnI GGCC 1 cut(s) 396
Bso31I GGTCTC 1 cut(s) 488
Bsp143I GATC 2 cut(s) 132, 504
Bsp19I CCATGG 1 cut(s) 701
BspANI GGCC 1 cut(s) 396
BspFNI CGCG 1 cut(s) 7
BspLI GGNNCC 2 cut(s) 326, 694
BspTNI GGTCTC 1 cut(s) 488
BsrFI RCCGGY 1 cut(s) 150
BsrI ACTGG 1 cut(s) 646
BssAI RCCGGY 1 cut(s) 150
BssECI CCNNGG 3 cut(s) 114, 701, 717
BssMI GATC 2 cut(s) 132, 504
BssNI GRCGYC 1 cut(s) 636
BssT1I CCWWGG 2 cut(s) 701, 717
Bst2UI CCWGG 2 cut(s) 115, 632
Bst4CI ACNGT 2 cut(s) 176, 324
Bst6I CTCTTC 3 cut(s) 23, 345, 755
BstACI GRCGYC 1 cut(s) 636
BstAPI GCANNNNNTGC 1 cut(s) 55
BstC8I GCNNGC 1 cut(s) 768
BstDEI CTNAG 2 cut(s) 756, 792
BstDSI CCRYGG 1 cut(s) 701
BstENI CCTNNNNNAGG 1 cut(s) 716
BstF5I GGATG 4 cut(s) 60, 268, 352, 405
BstFNI CGCG 1 cut(s) 7
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 2 cut(s) 135, 507
BstMAI GTCTC 4 cut(s) 274, 305, 488, 611
BstMBI GATC 2 cut(s) 132, 504
BstMWI GCNNNNNNNGC 3 cut(s) 55, 776, 785
BstNI CCWGG 2 cut(s) 115, 632
BstSCI CCNGG 2 cut(s) 113, 630
BstUI CGCG 1 cut(s) 7
BstV1I GCAGC 3 cut(s) 772, 775, 782
BstV2I GAAGAC 1 cut(s) 116
BstXI CCANNNNNNTGG 1 cut(s) 484
BsuRI GGCC 1 cut(s) 396
BtgI CCRYGG 1 cut(s) 701
BtsCI GGATG 4 cut(s) 60, 268, 352, 405
BtsIMutI CAGTG 1 cut(s) 639
Cac8I GCNNGC 1 cut(s) 768
CaiI CAGNNNCTG 1 cut(s) 788
CfoI GCGC 1 cut(s) 7
Cfr10I RCCGGY 1 cut(s) 150
Cfr13I GGNCC 3 cut(s) 101, 376, 693
CseI GACGC 1 cut(s) 644
CspAI ACCGGT 1 cut(s) 150
CspCI CAANNNNNGTGG 2 cut(s) 52, 87
CviAII CATG 6 cut(s) 206, 259, 544, 626, 647, 702
DdeI CTNAG 2 cut(s) 756, 792
DpnI GATC 2 cut(s) 134, 506
DpnII GATC 2 cut(s) 132, 504
DrdI GACNNNNNNGTC 1 cut(s) 327
DseDI GACNNNNNNGTC 1 cut(s) 327
EaeI YGGCCR 1 cut(s) 394
Eam1104I CTCTTC 3 cut(s) 23, 345, 755
EarI CTCTTC 3 cut(s) 23, 345, 755
Eco130I CCWWGG 2 cut(s) 701, 717
Eco31I GGTCTC 1 cut(s) 488
Eco47I GGWCC 3 cut(s) 101, 376, 693
Eco57I CTGAAG 1 cut(s) 35
EcoNI CCTNNNNNAGG 1 cut(s) 716
EcoRII CCWGG 2 cut(s) 113, 630
EcoT14I CCWWGG 2 cut(s) 701, 717
ErhI CCWWGG 2 cut(s) 701, 717
Esp3I CGTCTC 1 cut(s) 305
FaeI CATG 6 cut(s) 209, 262, 547, 629, 650, 705
FalI AAGNNNNNCTT 2 cut(s) 596, 628
FaqI GGGAC 1 cut(s) 706
FatI CATG 6 cut(s) 205, 258, 543, 625, 646, 701
FbaI TGATCA 1 cut(s) 132
FblI GTMKAC 1 cut(s) 510
Fnu4HI GCNGC 3 cut(s) 771, 786, 789
FokI GGATG 4 cut(s) 67, 275, 359, 392
Fsp4HI GCNGC 3 cut(s) 771, 786, 789
GlaI GCGC 1 cut(s) 6
GluI GCNGC 3 cut(s) 771, 786, 789
HaeIII GGCC 1 cut(s) 396
HapII CCGG 1 cut(s) 151
HgaI GACGC 1 cut(s) 644
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 636
Hin1II CATG 6 cut(s) 209, 262, 547, 629, 650, 705
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 1 cut(s) 511
HindII GTYRAC 1 cut(s) 511
HindIII AAGCTT 2 cut(s) 610, 734
HpaII CCGG 1 cut(s) 151
HphI GGTGA 1 cut(s) 559
Hpy166II GTNNAC 2 cut(s) 511, 678
Hpy188III TCNNGA 2 cut(s) 99, 586
Hpy8I GTNNAC 2 cut(s) 511, 678
Hpy99I CGWCG 1 cut(s) 512
HpyAV CCTTC 2 cut(s) 258, 722
HpyCH4III ACNGT 2 cut(s) 176, 324
HpyCH4IV ACGT 1 cut(s) 202
HpyCH4V TGCA 6 cut(s) 77, 199, 334, 372, 388, 656
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 776, 785
HpyF3I CTNAG 2 cut(s) 756, 792
HpySE526I ACGT 1 cut(s) 202
Hsp92I GRCGYC 1 cut(s) 636
Hsp92II CATG 6 cut(s) 209, 262, 547, 629, 650, 705
HspAI GCGC 1 cut(s) 5
Ksp22I TGATCA 1 cut(s) 132
Kzo9I GATC 2 cut(s) 132, 504
Lsp1109I GCAGC 3 cut(s) 772, 775, 782
LweI GCATC 2 cut(s) 45, 186
MaeII ACGT 1 cut(s) 202
MaeIII GTNAC 2 cut(s) 443, 547
MalI GATC 2 cut(s) 134, 506
MboI GATC 2 cut(s) 132, 504
MboII GAAGA 6 cut(s) 28, 40, 116, 362, 444, 772
MluCI AATT 4 cut(s) 65, 292, 438, 650
MmeI TCCRAC 1 cut(s) 556
MroXI GAANNNNTTC 2 cut(s) 123, 674
MseI TTAA 2 cut(s) 417, 732
MslI CAYNNNNRTG 1 cut(s) 194
MspA1I CMGCKG 1 cut(s) 785
MspI CCGG 1 cut(s) 151
MspR9I CCNGG 2 cut(s) 115, 632
Mva1269I GAATGC 1 cut(s) 334
MvaI CCWGG 2 cut(s) 115, 632
MvnI CGCG 1 cut(s) 7
MwoI GCNNNNNNNGC 3 cut(s) 55, 776, 785
NcoI CCATGG 1 cut(s) 701
NdeII GATC 2 cut(s) 132, 504
NlaIII CATG 6 cut(s) 209, 262, 547, 629, 650, 705
NlaIV GGNNCC 2 cut(s) 326, 694
NmuCI GTSAC 1 cut(s) 547
PctI GAATGC 1 cut(s) 334
PdmI GAANNNNTTC 2 cut(s) 123, 674
PflMI CCANNNNNTGG 2 cut(s) 51, 702
PfoI TCCNGGA 1 cut(s) 630
PinAI ACCGGT 1 cut(s) 150
PkrI GCNGC 3 cut(s) 772, 787, 790
PshBI ATTAAT 1 cut(s) 417
Psp6I CCWGG 2 cut(s) 113, 630
PspGI CCWGG 2 cut(s) 113, 630
PspN4I GGNNCC 2 cut(s) 326, 694
PspPI GGNCC 3 cut(s) 101, 376, 693
PsrI GAACNNNNNNTAC 2 cut(s) 395, 427
PstNI CAGNNNCTG 1 cut(s) 788
PvuII CAGCTG 1 cut(s) 785
RseI CAYNNNNRTG 1 cut(s) 194
SalI GTCGAC 1 cut(s) 509
SaqAI TTAA 2 cut(s) 417, 732
SatI GCNGC 3 cut(s) 771, 786, 789
Sau3AI GATC 2 cut(s) 132, 504
Sau96I GGNCC 3 cut(s) 101, 376, 693
ScrFI CCNGG 2 cut(s) 115, 632
SfaNI GCATC 2 cut(s) 45, 186
SinI GGWCC 3 cut(s) 101, 376, 693
SmiMI CAYNNNNRTG 1 cut(s) 194
Sse9I AATT 4 cut(s) 65, 292, 438, 650
StyD4I CCNGG 2 cut(s) 113, 630
StyI CCWWGG 2 cut(s) 701, 717
TaaI ACNGT 2 cut(s) 176, 324
TaiI ACGT 1 cut(s) 205
TaqI TCGA 1 cut(s) 510
TasI AATT 4 cut(s) 65, 292, 438, 650
Tru1I TTAA 2 cut(s) 417, 732
Tru9I TTAA 2 cut(s) 417, 732
TscAI CASTG 1 cut(s) 646
TseFI GTSAC 1 cut(s) 547
TseI GCWGC 3 cut(s) 770, 785, 788
Tsp45I GTSAC 1 cut(s) 547
TspDTI ATGAA 3 cut(s) 247, 363, 663
TspGWI ACGGA 4 cut(s) 117, 287, 317, 368
TspRI CASTG 1 cut(s) 646
Van91I CCANNNNNTGG 2 cut(s) 51, 702
VpaK11BI GGWCC 3 cut(s) 101, 376, 693
VspI ATTAAT 1 cut(s) 417
XagI CCTNNNNNAGG 1 cut(s) 716
XapI RAATTY 2 cut(s) 292, 650
XcmI CCANNNNNNNNNTGG 1 cut(s) 638
XmiI GTMKAC 1 cut(s) 510
XmnI GAANNNNTTC 2 cut(s) 123, 674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.