FvH4_7g25090

Belongs to the eukaryotic ribosomal protein eS4 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0001275
Physical Location & Seq
Forward (+)
19212276 .. 19215192
2917 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g25090.t2

Sequence Viewer

Length: 795 bp
ATGGCGAGGGGATTGAAGAAGCATTTGAAGAGGCTCAATGCCCCCAAGCATTGGATGCTTGACAAACTTGGTGGCGCATTTGCACCTAAGCCGTCATCTGGACCTCACAAGTCCAGGGAATGCCTGCCATTGATCATTATCCTGCGAAACCGGTTGAAATATGCTCTCACTTACCGTGAGGTCATTGCCATTCTGATGCAACGACATGTTCTGGTTGATGGGAAGGTTAGGACTGACAAAACCTATCCATCAGGTTTCATGGATGTTATATCAATCCCAAAAACAAATGAGAATTTCCGTCTCCTGTATGACACAAAGGGTCGGTTCCGTCTCCACTCTATCAGGGATGAAGAGTCAAAGTTTAAACTGTGCAAAGTCCGGTCAGTGCAGTTTGGTCAAAAGAATATCCCCTACATTAATACCTATGATGGGCGTACAATCCGCTACCCTGACCCTCTCATCAAGGCCAATGACACCATCAAGCTTGACCTAGAGACCAACAAGATCACCGACTTCATCAAGTTTGATGTTGGTAATGTGGTCATGGTCACTGGTGGAAGGAACAGGGGGCGTGTTGGTGTCATCAAGAGCAGGGAGAAGCACAAGGGAAGTTTTGAGACCATCCACGTCCAGGATTCAACTGGTCATGAGTTTGCAACCCGTTTGGGGAATGTGTTCACCATTGGCAAGGGAACAAAGCCATGGGTGAGTCTGCCCAAGGGAAGAGGTATTAAGCTATCGATCATCGAGGAGGCCAGAAAGAGGCAAGCAGCTCTACAAGCATCAGCCGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
40S_S4_C KOW RS4NT Ribosomal_S4e S4

Protein Analysis

265

Amino Acids

29.99

Weight (kDa)

10.32

Isoelectric Point (pI)

27.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RS4NT PF08071 1 - 21 2.6e-06 RS4NT (NUC023) domain
Ribosomal_S4e PF00900 77 - 151 1.4e-33 Ribosomal family S4e
KOW PF00467 159 - 192 7.2e-07 KOW motif
40S_S4_C PF16121 194 - 239 1e-24 40S ribosomal protein S4 C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 51
AciI CCGC 2 cut(s) 442, 789
AcsI RAATTY 1 cut(s) 292
AfaI GTAC 1 cut(s) 436
AfiI CCNNNNNNNGG 6 cut(s) 51, 98, 429, 631, 666, 762
AflIII ACRYGT 1 cut(s) 205
AgeI ACCGGT 1 cut(s) 150
AgsI TTSAA 4 cut(s) 16, 28, 157, 639
AjiI CACGTC 1 cut(s) 628
AjnI CCWGG 2 cut(s) 113, 630
AluBI AGCT 3 cut(s) 484, 736, 773
AluI AGCT 3 cut(s) 484, 736, 773
Alw26I GTCTC 4 cut(s) 305, 335, 488, 611
AoxI GGCC 2 cut(s) 465, 753
ApeKI GCWGC 1 cut(s) 770
ApoI RAATTY 1 cut(s) 292
AseI ATTAAT 1 cut(s) 417
AsiGI ACCGGT 1 cut(s) 150
Asp700I GAANNNNTTC 1 cut(s) 674
AspLEI GCGC 1 cut(s) 77
AspS9I GGNCC 1 cut(s) 101
AsuHPI GGTGA 3 cut(s) 499, 670, 718
AvaII GGWCC 1 cut(s) 101
BbvI GCAGC 1 cut(s) 782
BccI CCATC 5 cut(s) 212, 256, 422, 485, 629
BceAI ACGGC 1 cut(s) 76
BciT130I CCWGG 2 cut(s) 115, 632
BclI TGATCA 1 cut(s) 132
BcoDI GTCTC 4 cut(s) 305, 335, 488, 611
BfaI CTAG 1 cut(s) 491
BisI GCNGC 2 cut(s) 771, 789
BlsI GCNGC 2 cut(s) 772, 790
Bme1390I CCNGG 2 cut(s) 115, 632
Bme18I GGWCC 1 cut(s) 101
BmgBI CACGTC 1 cut(s) 628
BmgT120I GGNCC 1 cut(s) 101
BmiI GGNNCC 1 cut(s) 326
BmrFI CCNGG 2 cut(s) 115, 632
BmsI GCATC 3 cut(s) 45, 186, 791
Bpu10I CCTNAGC 1 cut(s) 87
Bsa29I ATCGAT 1 cut(s) 740
BsaBI GATNNNNATC 1 cut(s) 137
BsaI GGTCTC 2 cut(s) 488, 611
BsaJI CCNNGG 3 cut(s) 114, 701, 717
BsaWI WCCGGW 2 cut(s) 150, 378
Bsc4I CCNNNNNNNGG 6 cut(s) 51, 98, 429, 631, 666, 762
Bse118I RCCGGY 1 cut(s) 150
Bse1I ACTGG 2 cut(s) 556, 646
Bse3DI GCAATG 1 cut(s) 183
Bse8I GATNNNNATC 1 cut(s) 137
BseBI CCWGG 2 cut(s) 115, 632
BseCI ATCGAT 1 cut(s) 740
BseDI CCNNGG 3 cut(s) 114, 701, 717
BseGI GGATG 4 cut(s) 60, 268, 352, 621
BseJI GATNNNNATC 1 cut(s) 137
BseLI CCNNNNNNNGG 6 cut(s) 51, 98, 429, 631, 666, 762
BseMI GCAATG 1 cut(s) 183
BseNI ACTGG 2 cut(s) 556, 646
BseRI GAGGAG 1 cut(s) 764
BseXI GCAGC 1 cut(s) 782
BsgI GTGCAG 1 cut(s) 407
BshFI GGCC 2 cut(s) 467, 755
BshTI ACCGGT 1 cut(s) 150
BshVI ATCGAT 1 cut(s) 740
BsiSI CCGG 2 cut(s) 151, 379
BslI CCNNNNNNNGG 6 cut(s) 51, 98, 429, 631, 666, 762
BsmAI GTCTC 4 cut(s) 305, 335, 488, 611
BsmBI CGTCTC 2 cut(s) 305, 335
BsmI GAATGC 1 cut(s) 125
BsnI GGCC 2 cut(s) 467, 755
Bso31I GGTCTC 2 cut(s) 488, 611
Bsp143I GATC 3 cut(s) 132, 504, 741
Bsp19I CCATGG 1 cut(s) 701
BspACI CCGC 2 cut(s) 442, 789
BspANI GGCC 2 cut(s) 467, 755
BspDI ATCGAT 1 cut(s) 740
BspHI TCATGA 1 cut(s) 646
BspLI GGNNCC 1 cut(s) 326
BspTNI GGTCTC 2 cut(s) 488, 611
BsrDI GCAATG 1 cut(s) 183
BsrFI RCCGGY 1 cut(s) 150
BsrI ACTGG 2 cut(s) 556, 646
BssAI RCCGGY 1 cut(s) 150
BssECI CCNNGG 3 cut(s) 114, 701, 717
BssMI GATC 3 cut(s) 132, 504, 741
BssT1I CCWWGG 2 cut(s) 701, 717
Bst2UI CCWGG 2 cut(s) 115, 632
Bst4CI ACNGT 2 cut(s) 176, 369
Bst6I CTCTTC 3 cut(s) 23, 345, 718
BstAPI GCANNNNNTGC 1 cut(s) 55
BstC8I GCNNGC 2 cut(s) 125, 768
BstDEI CTNAG 1 cut(s) 87
BstDSI CCRYGG 1 cut(s) 701
BstF5I GGATG 4 cut(s) 60, 268, 352, 621
BstHHI GCGC 1 cut(s) 77
BstKTI GATC 3 cut(s) 135, 507, 744
BstMAI GTCTC 4 cut(s) 305, 335, 488, 611
BstMBI GATC 3 cut(s) 132, 504, 741
BstMWI GCNNNNNNNGC 3 cut(s) 55, 779, 788
BstNI CCWGG 2 cut(s) 115, 632
BstNSI RCATGY 1 cut(s) 209
BstSCI CCNGG 2 cut(s) 113, 630
BstV1I GCAGC 1 cut(s) 782
Bsu15I ATCGAT 1 cut(s) 740
BsuRI GGCC 2 cut(s) 467, 755
BsuTUI ATCGAT 1 cut(s) 740
BtgI CCRYGG 1 cut(s) 701
BtrI CACGTC 1 cut(s) 628
BtsCI GGATG 4 cut(s) 60, 268, 352, 621
BtsIMutI CAGTG 2 cut(s) 390, 549
Cac8I GCNNGC 2 cut(s) 125, 768
CciI TCATGA 1 cut(s) 646
CfoI GCGC 1 cut(s) 77
Cfr10I RCCGGY 1 cut(s) 150
Cfr13I GGNCC 1 cut(s) 101
ClaI ATCGAT 1 cut(s) 740
Csp6I GTAC 1 cut(s) 435
CspAI ACCGGT 1 cut(s) 150
CspCI CAANNNNNGTGG 2 cut(s) 52, 87
CviAII CATG 5 cut(s) 206, 259, 544, 647, 702
CviJI RGCY 9 cut(s) 34, 91, 467, 484, 700, 736, 755, 773, 788
CviKI_1 RGCY 9 cut(s) 34, 91, 467, 484, 700, 736, 755, 773, 788
CviQI GTAC 1 cut(s) 435
DdeI CTNAG 1 cut(s) 87
DpnI GATC 3 cut(s) 134, 506, 743
DpnII GATC 3 cut(s) 132, 504, 741
DraI TTTAAA 1 cut(s) 364
Eam1104I CTCTTC 3 cut(s) 23, 345, 718
EarI CTCTTC 3 cut(s) 23, 345, 718
Eco130I CCWWGG 2 cut(s) 701, 717
Eco31I GGTCTC 2 cut(s) 488, 611
Eco47I GGWCC 1 cut(s) 101
EcoRII CCWGG 2 cut(s) 113, 630
EcoT14I CCWWGG 2 cut(s) 701, 717
ErhI CCWWGG 2 cut(s) 701, 717
Esp3I CGTCTC 2 cut(s) 305, 335
FaeI CATG 5 cut(s) 209, 262, 547, 650, 705
FatI CATG 5 cut(s) 205, 258, 543, 646, 701
FbaI TGATCA 1 cut(s) 132
Fnu4HI GCNGC 2 cut(s) 771, 789
FokI GGATG 4 cut(s) 67, 275, 359, 608
Fsp4HI GCNGC 2 cut(s) 771, 789
FspBI CTAG 1 cut(s) 491
GlaI GCGC 1 cut(s) 76
GluI GCNGC 2 cut(s) 771, 789
HaeIII GGCC 2 cut(s) 467, 755
HapII CCGG 2 cut(s) 151, 379
HhaI GCGC 1 cut(s) 77
Hin1II CATG 5 cut(s) 209, 262, 547, 650, 705
Hin6I GCGC 1 cut(s) 75
HinP1I GCGC 1 cut(s) 75
HindIII AAGCTT 1 cut(s) 482
HinfI GANTC 3 cut(s) 353, 635, 709
HpaII CCGG 2 cut(s) 151, 379
HphI GGTGA 3 cut(s) 499, 670, 718
Hpy166II GTNNAC 1 cut(s) 678
Hpy188I TCNGA 1 cut(s) 195
Hpy188III TCNNGA 3 cut(s) 99, 586, 647
Hpy8I GTNNAC 1 cut(s) 678
HpyAV CCTTC 2 cut(s) 217, 552
HpyCH4III ACNGT 2 cut(s) 176, 369
HpyCH4IV ACGT 1 cut(s) 627
HpyCH4V TGCA 5 cut(s) 83, 199, 372, 388, 656
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 779, 788
HpyF3I CTNAG 1 cut(s) 87
HpySE526I ACGT 1 cut(s) 627
Hsp92II CATG 5 cut(s) 209, 262, 547, 650, 705
HspAI GCGC 1 cut(s) 75
Ksp22I TGATCA 1 cut(s) 132
Kzo9I GATC 3 cut(s) 132, 504, 741
Lsp1109I GCAGC 1 cut(s) 782
LweI GCATC 3 cut(s) 45, 186, 791
MaeI CTAG 1 cut(s) 491
MaeII ACGT 1 cut(s) 627
MaeIII GTNAC 1 cut(s) 547
MalI GATC 3 cut(s) 134, 506, 743
MboI GATC 3 cut(s) 132, 504, 741
MboII GAAGA 4 cut(s) 28, 40, 362, 735
MluCI AATT 1 cut(s) 292
MlyI GAGTC 2 cut(s) 362, 718
MnlI CCTC 8 cut(s) 24, 114, 172, 465, 719, 742, 745, 756
MroXI GAANNNNTTC 1 cut(s) 674
MseI TTAA 3 cut(s) 363, 417, 732
MslI CAYNNNNRTG 1 cut(s) 194
MspI CCGG 2 cut(s) 151, 379
MspR9I CCNGG 2 cut(s) 115, 632
MssI GTTTAAAC 1 cut(s) 364
Mva1269I GAATGC 1 cut(s) 125
MvaI CCWGG 2 cut(s) 115, 632
MwoI GCNNNNNNNGC 3 cut(s) 55, 779, 788
NcoI CCATGG 1 cut(s) 701
NdeII GATC 3 cut(s) 132, 504, 741
NlaIII CATG 5 cut(s) 209, 262, 547, 650, 705
NlaIV GGNNCC 1 cut(s) 326
NmuCI GTSAC 1 cut(s) 547
NspI RCATGY 1 cut(s) 209
PagI TCATGA 1 cut(s) 646
PciI ACATGT 1 cut(s) 205
PctI GAATGC 1 cut(s) 125
PdmI GAANNNNTTC 1 cut(s) 674
PfeI GAWTC 1 cut(s) 635
PflMI CCANNNNNTGG 1 cut(s) 51
PfoI TCCNGGA 1 cut(s) 630
PinAI ACCGGT 1 cut(s) 150
PkrI GCNGC 2 cut(s) 772, 790
PleI GAGTC 2 cut(s) 361, 717
PmeI GTTTAAAC 1 cut(s) 364
PpsI GAGTC 2 cut(s) 361, 717
PscI ACATGT 1 cut(s) 205
PshBI ATTAAT 1 cut(s) 417
Psp6I CCWGG 2 cut(s) 113, 630
PspGI CCWGG 2 cut(s) 113, 630
PspN4I GGNNCC 1 cut(s) 326
PspPI GGNCC 1 cut(s) 101
RsaI GTAC 1 cut(s) 436
RsaNI GTAC 1 cut(s) 435
RseI CAYNNNNRTG 1 cut(s) 194
SaqAI TTAA 3 cut(s) 363, 417, 732
SatI GCNGC 2 cut(s) 771, 789
Sau3AI GATC 3 cut(s) 132, 504, 741
Sau96I GGNCC 1 cut(s) 101
SchI GAGTC 2 cut(s) 362, 718
ScrFI CCNGG 2 cut(s) 115, 632
SfaNI GCATC 3 cut(s) 45, 186, 791
SinI GGWCC 1 cut(s) 101
SmiMI CAYNNNNRTG 1 cut(s) 194
Sse9I AATT 1 cut(s) 292
SsiI CCGC 2 cut(s) 442, 789
SspMI CTAG 1 cut(s) 491
StyD4I CCNGG 2 cut(s) 113, 630
StyI CCWWGG 2 cut(s) 701, 717
TaaI ACNGT 2 cut(s) 176, 369
TaiI ACGT 1 cut(s) 630
TaqI TCGA 2 cut(s) 740, 747
TasI AATT 1 cut(s) 292
TauI GCSGC 1 cut(s) 791
TfiI GAWTC 1 cut(s) 635
Tru1I TTAA 3 cut(s) 363, 417, 732
Tru9I TTAA 3 cut(s) 363, 417, 732
TscAI CASTG 2 cut(s) 390, 556
TseFI GTSAC 1 cut(s) 547
TseI GCWGC 1 cut(s) 770
Tsp45I GTSAC 1 cut(s) 547
TspDTI ATGAA 3 cut(s) 247, 363, 505
TspGWI ACGGA 2 cut(s) 287, 317
TspRI CASTG 2 cut(s) 390, 556
Van91I CCANNNNNTGG 1 cut(s) 51
VpaK11BI GGWCC 1 cut(s) 101
VspI ATTAAT 1 cut(s) 417
XapI RAATTY 1 cut(s) 292
XceI RCATGY 1 cut(s) 209
XcmI CCANNNNNNNNNTGG 1 cut(s) 638
XmnI GAANNNNTTC 1 cut(s) 674
XspI CTAG 1 cut(s) 491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.