Rroxscaffold_1G00056930

Belongs to the eukaryotic ribosomal protein eS4 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001275
Physical Location & Seq
Forward (+)
78919765 .. 78922060
2296 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00056930.1

Sequence Viewer

Length: 849 bp
ATGGCGCGAGGACTGAAGAAGCATTTGAAGAGGCTCAATGCCCCCAAGCATTGGATGCTTGACAAATTGGGTGGTGCATTTGCCCCCAAGCCCTCATCTGGACCTCACAAATCCAGGGAATGTCTTCCTTTGATCATTATCTTGCGAAACCGGTTGAAGTATGCTTTGACATACCGTGAGGTCATTGCCATCTTGATGCAACGTCATGTTATGGTCGATGGGAAAGTTAGGACTGATAAGACTTATCCTTCTGGTTTCATGGTGGTCTTGATTTTGGAATGTGGCACGTTATTAACATTCTGCTTTACTTTGACAGATGTTGTCTCAATCCCCAAGACAAATGAGAATTTCCGTCTCCTTTATGACACCAAAGGACGGTTCCGTCTGCATTCAATCAGGGATGAAGAGGCAAAGTTCAAACTCTGCAAGGTCCGTTCTGTGCAGTTCGGCCAAAAGAACATCCCTTATATTAATACCTATGATGGAAGAACAATTCGTTACCCTGACCCTCTCATCAAGGCAAATGACACCATCAAGCTGGATTTGGAGACCAACAAGATCGTCGACTTTATCAAGTTTGATGTTGGTAATGTTGTCATGGTGACAGGTGGTAGGAACAGAGGGCGAGTTGGAGTAATCAAGAACAGGGAGAAGCATAAGGGAAGCTTTGAGACTATCCATGTCCAGGACGCCACTGGACATGAATTTGCAACCCGTTTGGCGAATGTGTTCACGATTGGTAAGGGGACGAAGCCATGGGTAAGCCTTCCCAAGGGTAAGGGTATTAAGCTTACCATCATTGAGGAGGCCAAGAAGAGGCAAGCAGCCCAAGCCACAGCTGCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
40S_S4_C KOW RS4NT Ribosomal_S4e S4

Protein Analysis

282

Amino Acids

31.9

Weight (kDa)

10.12

Isoelectric Point (pI)

24.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RS4NT PF08071 3 - 39 2.6e-18 RS4NT (NUC023) domain
Ribosomal_S4e PF00900 113 - 187 5e-34 Ribosomal family S4e
KOW PF00467 195 - 228 2e-06 KOW motif
40S_S4_C PF16121 230 - 276 7.6e-26 40S ribosomal protein S4 C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 381
AccB7I CCANNNNNTGG 1 cut(s) 51
AccI GTMKAC 1 cut(s) 564
AccII CGCG 1 cut(s) 7
AcoI YGGCCR 1 cut(s) 448
AcsI RAATTY 2 cut(s) 346, 704
AcuI CTGAAG 1 cut(s) 35
AcyI GRCGYC 1 cut(s) 690
AfiI CCNNNNNNNGG 6 cut(s) 51, 98, 375, 685, 772, 816
AgeI ACCGGT 1 cut(s) 150
AgsI TTSAA 4 cut(s) 28, 157, 393, 418
AjnI CCWGG 2 cut(s) 113, 684
AjuI GAANNNNNNNTTGG 2 cut(s) 362, 394
AluBI AGCT 4 cut(s) 538, 666, 790, 839
AluI AGCT 4 cut(s) 538, 666, 790, 839
Alw26I GTCTC 4 cut(s) 328, 359, 542, 665
AlwNI CAGNNNCTG 1 cut(s) 842
AoxI GGCC 2 cut(s) 448, 807
ApeKI GCWGC 3 cut(s) 824, 839, 842
ApoI RAATTY 2 cut(s) 346, 704
AseI ATTAAT 1 cut(s) 471
AsiGI ACCGGT 1 cut(s) 150
Asp700I GAANNNNTTC 2 cut(s) 123, 728
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 2 cut(s) 101, 430
AsuHPI GGTGA 1 cut(s) 613
AvaII GGWCC 2 cut(s) 101, 430
BbsI GAAGAC 1 cut(s) 116
BbvI GCAGC 3 cut(s) 826, 829, 836
BccI CCATC 5 cut(s) 197, 212, 476, 539, 803
BciT130I CCWGG 2 cut(s) 115, 686
BclI TGATCA 1 cut(s) 132
BcoDI GTCTC 4 cut(s) 328, 359, 542, 665
BisI GCNGC 3 cut(s) 825, 840, 843
BlsI GCNGC 3 cut(s) 826, 841, 844
Bme1390I CCNGG 2 cut(s) 115, 686
Bme18I GGWCC 2 cut(s) 101, 430
BmgT120I GGNCC 2 cut(s) 101, 430
BmiI GGNNCC 1 cut(s) 380
BmrFI CCNGG 2 cut(s) 115, 686
BmsI GCATC 2 cut(s) 45, 186
BpiI GAAGAC 1 cut(s) 116
BsaBI GATNNNNATC 1 cut(s) 137
BsaHI GRCGYC 1 cut(s) 690
BsaI GGTCTC 1 cut(s) 542
BsaJI CCNNGG 3 cut(s) 114, 755, 771
BsaWI WCCGGW 1 cut(s) 150
Bsc4I CCNNNNNNNGG 6 cut(s) 51, 98, 375, 685, 772, 816
Bse118I RCCGGY 1 cut(s) 150
Bse1I ACTGG 1 cut(s) 700
Bse3DI GCAATG 1 cut(s) 183
Bse8I GATNNNNATC 1 cut(s) 137
BseBI CCWGG 2 cut(s) 115, 686
BseDI CCNNGG 3 cut(s) 114, 755, 771
BseGI GGATG 3 cut(s) 60, 406, 459
BseJI GATNNNNATC 1 cut(s) 137
BseLI CCNNNNNNNGG 6 cut(s) 51, 98, 375, 685, 772, 816
BseMI GCAATG 1 cut(s) 183
BseNI ACTGG 1 cut(s) 700
BseRI GAGGAG 1 cut(s) 818
BseXI GCAGC 3 cut(s) 826, 829, 836
BsgI GTGCAG 1 cut(s) 461
Bsh1236I CGCG 1 cut(s) 7
BshFI GGCC 2 cut(s) 450, 809
BshTI ACCGGT 1 cut(s) 150
BsiSI CCGG 1 cut(s) 151
BslFI GGGAC 1 cut(s) 760
BslI CCNNNNNNNGG 6 cut(s) 51, 98, 375, 685, 772, 816
BsmAI GTCTC 4 cut(s) 328, 359, 542, 665
BsmBI CGTCTC 1 cut(s) 359
BsmFI GGGAC 1 cut(s) 760
BsmI GAATGC 1 cut(s) 388
BsnI GGCC 2 cut(s) 450, 809
Bso31I GGTCTC 1 cut(s) 542
Bsp143I GATC 2 cut(s) 132, 558
Bsp19I CCATGG 1 cut(s) 755
BspANI GGCC 2 cut(s) 450, 809
BspFNI CGCG 1 cut(s) 7
BspLI GGNNCC 1 cut(s) 380
BspTNI GGTCTC 1 cut(s) 542
BsrDI GCAATG 1 cut(s) 183
BsrFI RCCGGY 1 cut(s) 150
BsrI ACTGG 1 cut(s) 700
BssAI RCCGGY 1 cut(s) 150
BssECI CCNNGG 3 cut(s) 114, 755, 771
BssMI GATC 2 cut(s) 132, 558
BssNI GRCGYC 1 cut(s) 690
BssT1I CCWWGG 2 cut(s) 755, 771
Bst2UI CCWGG 2 cut(s) 115, 686
Bst4CI ACNGT 2 cut(s) 176, 378
Bst6I CTCTTC 3 cut(s) 23, 399, 809
BstACI GRCGYC 1 cut(s) 690
BstAPI GCANNNNNTGC 1 cut(s) 55
BstC8I GCNNGC 1 cut(s) 822
BstDEI CTNAG 1 cut(s) 846
BstDSI CCRYGG 1 cut(s) 755
BstENI CCTNNNNNAGG 1 cut(s) 770
BstF5I GGATG 3 cut(s) 60, 406, 459
BstFNI CGCG 1 cut(s) 7
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 2 cut(s) 135, 561
BstMAI GTCTC 4 cut(s) 328, 359, 542, 665
BstMBI GATC 2 cut(s) 132, 558
BstMWI GCNNNNNNNGC 3 cut(s) 55, 830, 839
BstNI CCWGG 2 cut(s) 115, 686
BstSCI CCNGG 2 cut(s) 113, 684
BstUI CGCG 1 cut(s) 7
BstV1I GCAGC 3 cut(s) 826, 829, 836
BstV2I GAAGAC 1 cut(s) 116
BstXI CCANNNNNNTGG 1 cut(s) 538
BsuRI GGCC 2 cut(s) 450, 809
BtgI CCRYGG 1 cut(s) 755
BtsCI GGATG 3 cut(s) 60, 406, 459
BtsIMutI CAGTG 1 cut(s) 693
Cac8I GCNNGC 1 cut(s) 822
CaiI CAGNNNCTG 1 cut(s) 842
CfoI GCGC 1 cut(s) 7
Cfr10I RCCGGY 1 cut(s) 150
Cfr13I GGNCC 2 cut(s) 101, 430
CseI GACGC 1 cut(s) 698
CspAI ACCGGT 1 cut(s) 150
CspCI CAANNNNNGTGG 2 cut(s) 52, 87
CviAII CATG 6 cut(s) 206, 259, 598, 680, 701, 756
DdeI CTNAG 1 cut(s) 846
DpnI GATC 2 cut(s) 134, 560
DpnII GATC 2 cut(s) 132, 558
DrdI GACNNNNNNGTC 1 cut(s) 381
DseDI GACNNNNNNGTC 1 cut(s) 381
EaeI YGGCCR 1 cut(s) 448
Eam1104I CTCTTC 3 cut(s) 23, 399, 809
EarI CTCTTC 3 cut(s) 23, 399, 809
Eco130I CCWWGG 2 cut(s) 755, 771
Eco31I GGTCTC 1 cut(s) 542
Eco47I GGWCC 2 cut(s) 101, 430
Eco57I CTGAAG 1 cut(s) 35
EcoNI CCTNNNNNAGG 1 cut(s) 770
EcoRII CCWGG 2 cut(s) 113, 684
EcoT14I CCWWGG 2 cut(s) 755, 771
ErhI CCWWGG 2 cut(s) 755, 771
Esp3I CGTCTC 1 cut(s) 359
FaeI CATG 6 cut(s) 209, 262, 601, 683, 704, 759
FalI AAGNNNNNCTT 2 cut(s) 650, 682
FaqI GGGAC 1 cut(s) 760
FatI CATG 6 cut(s) 205, 258, 597, 679, 700, 755
FbaI TGATCA 1 cut(s) 132
FblI GTMKAC 1 cut(s) 564
Fnu4HI GCNGC 3 cut(s) 825, 840, 843
FokI GGATG 3 cut(s) 67, 413, 446
Fsp4HI GCNGC 3 cut(s) 825, 840, 843
GlaI GCGC 1 cut(s) 6
GluI GCNGC 3 cut(s) 825, 840, 843
HaeIII GGCC 2 cut(s) 450, 809
HapII CCGG 1 cut(s) 151
HgaI GACGC 1 cut(s) 698
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 690
Hin1II CATG 6 cut(s) 209, 262, 601, 683, 704, 759
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 1 cut(s) 565
HindII GTYRAC 1 cut(s) 565
HindIII AAGCTT 2 cut(s) 664, 788
HpaII CCGG 1 cut(s) 151
HphI GGTGA 1 cut(s) 613
Hpy166II GTNNAC 2 cut(s) 565, 732
Hpy188III TCNNGA 5 cut(s) 99, 193, 268, 640, 733
Hpy8I GTNNAC 2 cut(s) 565, 732
Hpy99I CGWCG 1 cut(s) 566
HpyAV CCTTC 2 cut(s) 258, 776
HpyCH4III ACNGT 2 cut(s) 176, 378
HpyCH4IV ACGT 2 cut(s) 202, 287
HpyCH4V TGCA 6 cut(s) 77, 199, 388, 426, 442, 710
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 830, 839
HpyF3I CTNAG 1 cut(s) 846
HpySE526I ACGT 2 cut(s) 202, 287
Hsp92I GRCGYC 1 cut(s) 690
Hsp92II CATG 6 cut(s) 209, 262, 601, 683, 704, 759
HspAI GCGC 1 cut(s) 5
Ksp22I TGATCA 1 cut(s) 132
Kzo9I GATC 2 cut(s) 132, 558
Lsp1109I GCAGC 3 cut(s) 826, 829, 836
LweI GCATC 2 cut(s) 45, 186
MaeII ACGT 2 cut(s) 202, 287
MaeIII GTNAC 2 cut(s) 497, 601
MalI GATC 2 cut(s) 134, 560
MboI GATC 2 cut(s) 132, 558
MboII GAAGA 6 cut(s) 28, 40, 116, 416, 498, 826
MluCI AATT 4 cut(s) 65, 346, 492, 704
MmeI TCCRAC 1 cut(s) 610
MroXI GAANNNNTTC 2 cut(s) 123, 728
MseI TTAA 3 cut(s) 293, 471, 786
MslI CAYNNNNRTG 1 cut(s) 194
MspA1I CMGCKG 1 cut(s) 839
MspI CCGG 1 cut(s) 151
MspR9I CCNGG 2 cut(s) 115, 686
Mva1269I GAATGC 1 cut(s) 388
MvaI CCWGG 2 cut(s) 115, 686
MvnI CGCG 1 cut(s) 7
MwoI GCNNNNNNNGC 3 cut(s) 55, 830, 839
NcoI CCATGG 1 cut(s) 755
NdeII GATC 2 cut(s) 132, 558
NlaIII CATG 6 cut(s) 209, 262, 601, 683, 704, 759
NlaIV GGNNCC 1 cut(s) 380
NmuCI GTSAC 1 cut(s) 601
PctI GAATGC 1 cut(s) 388
PdmI GAANNNNTTC 2 cut(s) 123, 728
PflMI CCANNNNNTGG 1 cut(s) 51
PfoI TCCNGGA 1 cut(s) 684
PinAI ACCGGT 1 cut(s) 150
PkrI GCNGC 3 cut(s) 826, 841, 844
PshBI ATTAAT 1 cut(s) 471
Psp6I CCWGG 2 cut(s) 113, 684
PspGI CCWGG 2 cut(s) 113, 684
PspN4I GGNNCC 1 cut(s) 380
PspPI GGNCC 2 cut(s) 101, 430
PstNI CAGNNNCTG 1 cut(s) 842
PvuII CAGCTG 1 cut(s) 839
RseI CAYNNNNRTG 1 cut(s) 194
SalI GTCGAC 1 cut(s) 563
SaqAI TTAA 3 cut(s) 293, 471, 786
SatI GCNGC 3 cut(s) 825, 840, 843
Sau3AI GATC 2 cut(s) 132, 558
Sau96I GGNCC 2 cut(s) 101, 430
ScrFI CCNGG 2 cut(s) 115, 686
SfaNI GCATC 2 cut(s) 45, 186
SinI GGWCC 2 cut(s) 101, 430
SmiMI CAYNNNNRTG 1 cut(s) 194
Sse9I AATT 4 cut(s) 65, 346, 492, 704
StyD4I CCNGG 2 cut(s) 113, 684
StyI CCWWGG 2 cut(s) 755, 771
TaaI ACNGT 2 cut(s) 176, 378
TaiI ACGT 2 cut(s) 205, 290
TaqI TCGA 2 cut(s) 216, 564
TasI AATT 4 cut(s) 65, 346, 492, 704
Tru1I TTAA 3 cut(s) 293, 471, 786
Tru9I TTAA 3 cut(s) 293, 471, 786
TscAI CASTG 1 cut(s) 700
TseFI GTSAC 1 cut(s) 601
TseI GCWGC 3 cut(s) 824, 839, 842
Tsp45I GTSAC 1 cut(s) 601
TspDTI ATGAA 3 cut(s) 247, 417, 717
TspGWI ACGGA 3 cut(s) 341, 371, 422
TspRI CASTG 1 cut(s) 700
Van91I CCANNNNNTGG 1 cut(s) 51
VpaK11BI GGWCC 2 cut(s) 101, 430
VspI ATTAAT 1 cut(s) 471
XagI CCTNNNNNAGG 1 cut(s) 770
XapI RAATTY 2 cut(s) 346, 704
XcmI CCANNNNNNNNNTGG 1 cut(s) 692
XmiI GTMKAC 1 cut(s) 564
XmnI GAANNNNTTC 2 cut(s) 123, 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.