Rh1AG370400

Belongs to the eukaryotic ribosomal protein eS4 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0001275
Physical Location & Seq
Forward (+)
60375712 .. 60378249
2538 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG370400.1

Sequence Viewer

Length: 741 bp
ATGCTTGACAAACTTGGTGGCGCATTTGCACCTAAGCCCTCATCTGGACCTCACAAGGCCAGGGAGTGCCTGCCGTTGATCATTATCTTGCGAAACCGGTTGAAATATGCTCTCACTTACCGTGAAGTCATTTCCATTTTGATGCAACGACATGTTCTGGTTGATGGGAAGGTTAGGACTGACAAAACCTATCCTTCAGGTTTCATGGATGTTATATCAATCCCGAAAACAAATGAGAATTTTCGTCTCCTTTATGACACAAAGGGTCGGTTCCGCCTCCACTCAATCAGGGATGAAGAGTCAAAGTTTAAACTGTGCAAAGTCCGCTCTGTACAGTTTGGGCAAAAGAATATCCCCTACATTAACACCTATGATGGGCGTACAATCCGCTACCCTGACCCTCTCATCAAGGCCAATGACACCATAAAGCTTGACCTAGAGACCAACAAGATCACCGACTTCATTAAGTTTGATGTTGGGAATGTGGTCATGGTTACTGGTGGAAGGAACAGGGGGCGTGTTGGTGTGATCAAGAACAGGGAGAAACACAAGGGAAGTTTTGAGACCATCCACGTCCAGGATGCAACTGGTCATGAGTTTGCAACCCGTTTGGCGAATGTGTTCACCATTGGCAAGGGAACAAAGCCATGGGTGAGTCTGCCCAAGGGAAGAGGTATTAAGCTCTCGATCATCGAGGAGGCCAGAAAGAGGCAAGCAGCTCAACAAGCATCCGCCGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
40S_S4_C KOW RS4NT Ribosomal_S4e S4

Protein Analysis

246

Amino Acids

27.87

Weight (kDa)

10.13

Isoelectric Point (pI)

23.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RS4NT PF08071 1 - 21 1.4e-06 RS4NT (NUC023) domain
Ribosomal_S4e PF00900 77 - 151 1.4e-33 Ribosomal family S4e
KOW PF00467 159 - 192 1.7e-06 KOW motif
40S_S4_C PF16121 194 - 240 5.3e-25 40S ribosomal protein S4 C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 327
AciI CCGC 5 cut(s) 274, 325, 388, 732, 735
AcsI RAATTY 1 cut(s) 238
AcuI CTGAAG 1 cut(s) 180
AfaI GTAC 2 cut(s) 333, 382
AfiI CCNNNNNNNGG 4 cut(s) 44, 375, 577, 708
AflIII ACRYGT 1 cut(s) 151
AgeI ACCGGT 1 cut(s) 96
AgsI TTSAA 1 cut(s) 103
AjiI CACGTC 1 cut(s) 574
AjnI CCWGG 2 cut(s) 59, 576
AluBI AGCT 3 cut(s) 430, 682, 719
AluI AGCT 3 cut(s) 430, 682, 719
Alw26I GTCTC 3 cut(s) 251, 434, 557
AoxI GGCC 3 cut(s) 57, 411, 699
ApeKI GCWGC 1 cut(s) 716
ApoI RAATTY 1 cut(s) 238
AsiGI ACCGGT 1 cut(s) 96
Asp700I GAANNNNTTC 1 cut(s) 620
AspLEI GCGC 1 cut(s) 23
AspS9I GGNCC 1 cut(s) 47
AsuHPI GGTGA 3 cut(s) 445, 616, 664
AvaII GGWCC 1 cut(s) 47
BbvI GCAGC 1 cut(s) 728
BccI CCATC 3 cut(s) 158, 368, 575
BceAI ACGGC 1 cut(s) 58
BciT130I CCWGG 2 cut(s) 61, 578
BclI TGATCA 2 cut(s) 78, 528
BcoDI GTCTC 3 cut(s) 251, 434, 557
BfaI CTAG 1 cut(s) 437
BisI GCNGC 2 cut(s) 717, 735
BlsI GCNGC 2 cut(s) 718, 736
Bme1390I CCNGG 2 cut(s) 61, 578
Bme18I GGWCC 1 cut(s) 47
BmgBI CACGTC 1 cut(s) 574
BmgT120I GGNCC 1 cut(s) 47
BmiI GGNNCC 1 cut(s) 272
BmrFI CCNGG 2 cut(s) 61, 578
BmsI GCATC 3 cut(s) 132, 571, 737
Bpu10I CCTNAGC 1 cut(s) 33
BsaBI GATNNNNATC 1 cut(s) 83
BsaI GGTCTC 2 cut(s) 434, 557
BsaJI CCNNGG 3 cut(s) 60, 647, 663
BsaWI WCCGGW 1 cut(s) 96
Bsc4I CCNNNNNNNGG 4 cut(s) 44, 375, 577, 708
Bse118I RCCGGY 1 cut(s) 96
Bse1I ACTGG 2 cut(s) 502, 592
Bse8I GATNNNNATC 1 cut(s) 83
BseBI CCWGG 2 cut(s) 61, 578
BseDI CCNNGG 3 cut(s) 60, 647, 663
BseGI GGATG 5 cut(s) 214, 298, 567, 586, 728
BseJI GATNNNNATC 1 cut(s) 83
BseLI CCNNNNNNNGG 4 cut(s) 44, 375, 577, 708
BseNI ACTGG 2 cut(s) 502, 592
BseRI GAGGAG 1 cut(s) 710
BseXI GCAGC 1 cut(s) 728
BshFI GGCC 3 cut(s) 59, 413, 701
BshTI ACCGGT 1 cut(s) 96
BsiSI CCGG 1 cut(s) 97
BslI CCNNNNNNNGG 4 cut(s) 44, 375, 577, 708
BsmAI GTCTC 3 cut(s) 251, 434, 557
BsmBI CGTCTC 1 cut(s) 251
BsnI GGCC 3 cut(s) 59, 413, 701
Bso31I GGTCTC 2 cut(s) 434, 557
Bsp1407I TGTACA 1 cut(s) 331
Bsp143I GATC 4 cut(s) 78, 450, 528, 687
Bsp19I CCATGG 1 cut(s) 647
BspACI CCGC 5 cut(s) 274, 325, 388, 732, 735
BspANI GGCC 3 cut(s) 59, 413, 701
BspHI TCATGA 1 cut(s) 592
BspLI GGNNCC 1 cut(s) 272
BspTNI GGTCTC 2 cut(s) 434, 557
BsrBI CCGCTC 1 cut(s) 327
BsrFI RCCGGY 1 cut(s) 96
BsrGI TGTACA 1 cut(s) 331
BsrI ACTGG 2 cut(s) 502, 592
BssAI RCCGGY 1 cut(s) 96
BssECI CCNNGG 3 cut(s) 60, 647, 663
BssMI GATC 4 cut(s) 78, 450, 528, 687
BssT1I CCWWGG 2 cut(s) 647, 663
Bst2UI CCWGG 2 cut(s) 61, 578
Bst4CI ACNGT 3 cut(s) 122, 315, 336
Bst6I CTCTTC 2 cut(s) 291, 664
BstAUI TGTACA 1 cut(s) 331
BstC8I GCNNGC 2 cut(s) 71, 714
BstDEI CTNAG 1 cut(s) 33
BstDSI CCRYGG 1 cut(s) 647
BstF5I GGATG 5 cut(s) 214, 298, 567, 586, 728
BstHHI GCGC 1 cut(s) 23
BstKTI GATC 4 cut(s) 81, 453, 531, 690
BstMAI GTCTC 3 cut(s) 251, 434, 557
BstMBI GATC 4 cut(s) 78, 450, 528, 687
BstMWI GCNNNNNNNGC 3 cut(s) 324, 725, 734
BstNI CCWGG 2 cut(s) 61, 578
BstNSI RCATGY 1 cut(s) 155
BstSCI CCNGG 2 cut(s) 59, 576
BstV1I GCAGC 1 cut(s) 728
BsuRI GGCC 3 cut(s) 59, 413, 701
BtgI CCRYGG 1 cut(s) 647
BtrI CACGTC 1 cut(s) 574
BtsCI GGATG 5 cut(s) 214, 298, 567, 586, 728
Cac8I GCNNGC 2 cut(s) 71, 714
CciI TCATGA 1 cut(s) 592
CfoI GCGC 1 cut(s) 23
Cfr10I RCCGGY 1 cut(s) 96
Cfr13I GGNCC 1 cut(s) 47
Csp6I GTAC 2 cut(s) 332, 381
CspAI ACCGGT 1 cut(s) 96
CspCI CAANNNNNGTGG 1 cut(s) 33
CviAII CATG 5 cut(s) 152, 205, 490, 593, 648
CviJI RGCY 8 cut(s) 37, 59, 413, 430, 646, 682, 701, 719
CviKI_1 RGCY 8 cut(s) 37, 59, 413, 430, 646, 682, 701, 719
CviQI GTAC 2 cut(s) 332, 381
DdeI CTNAG 1 cut(s) 33
DpnI GATC 4 cut(s) 80, 452, 530, 689
DpnII GATC 4 cut(s) 78, 450, 528, 687
DraI TTTAAA 1 cut(s) 310
Eam1104I CTCTTC 2 cut(s) 291, 664
EarI CTCTTC 2 cut(s) 291, 664
EciI GGCGGA 2 cut(s) 263, 721
Eco130I CCWWGG 2 cut(s) 647, 663
Eco31I GGTCTC 2 cut(s) 434, 557
Eco47I GGWCC 1 cut(s) 47
Eco57I CTGAAG 1 cut(s) 180
EcoRII CCWGG 2 cut(s) 59, 576
EcoT14I CCWWGG 2 cut(s) 647, 663
ErhI CCWWGG 2 cut(s) 647, 663
Esp3I CGTCTC 1 cut(s) 251
FaeI CATG 5 cut(s) 155, 208, 493, 596, 651
FatI CATG 5 cut(s) 151, 204, 489, 592, 647
FbaI TGATCA 2 cut(s) 78, 528
Fnu4HI GCNGC 2 cut(s) 717, 735
FokI GGATG 5 cut(s) 221, 305, 554, 593, 715
Fsp4HI GCNGC 2 cut(s) 717, 735
FspBI CTAG 1 cut(s) 437
GlaI GCGC 1 cut(s) 22
GluI GCNGC 2 cut(s) 717, 735
HaeIII GGCC 3 cut(s) 59, 413, 701
HapII CCGG 1 cut(s) 97
HhaI GCGC 1 cut(s) 23
Hin1II CATG 5 cut(s) 155, 208, 493, 596, 651
Hin6I GCGC 1 cut(s) 21
HinP1I GCGC 1 cut(s) 21
HindIII AAGCTT 1 cut(s) 428
HinfI GANTC 2 cut(s) 299, 655
HpaII CCGG 1 cut(s) 97
HphI GGTGA 3 cut(s) 445, 616, 664
Hpy166II GTNNAC 1 cut(s) 624
Hpy188III TCNNGA 5 cut(s) 45, 223, 532, 593, 685
Hpy8I GTNNAC 1 cut(s) 624
HpyAV CCTTC 3 cut(s) 163, 204, 498
HpyCH4III ACNGT 3 cut(s) 122, 315, 336
HpyCH4IV ACGT 1 cut(s) 573
HpyCH4V TGCA 5 cut(s) 29, 145, 318, 584, 602
HpyF10VI GCNNNNNNNGC 3 cut(s) 324, 725, 734
HpyF3I CTNAG 1 cut(s) 33
HpySE526I ACGT 1 cut(s) 573
Hsp92II CATG 5 cut(s) 155, 208, 493, 596, 651
HspAI GCGC 1 cut(s) 21
Ksp22I TGATCA 2 cut(s) 78, 528
Kzo9I GATC 4 cut(s) 78, 450, 528, 687
Lsp1109I GCAGC 1 cut(s) 728
LweI GCATC 3 cut(s) 132, 571, 737
MaeI CTAG 1 cut(s) 437
MaeII ACGT 1 cut(s) 573
MaeIII GTNAC 1 cut(s) 493
MalI GATC 4 cut(s) 80, 452, 530, 689
MbiI CCGCTC 1 cut(s) 327
MboI GATC 4 cut(s) 78, 450, 528, 687
MboII GAAGA 2 cut(s) 308, 681
MluCI AATT 1 cut(s) 238
MlyI GAGTC 2 cut(s) 308, 664
MnlI CCTC 8 cut(s) 49, 60, 287, 411, 665, 688, 691, 702
MroXI GAANNNNTTC 1 cut(s) 620
MseI TTAA 4 cut(s) 309, 363, 465, 678
MslI CAYNNNNRTG 1 cut(s) 140
MspI CCGG 1 cut(s) 97
MspR9I CCNGG 2 cut(s) 61, 578
MssI GTTTAAAC 1 cut(s) 310
MvaI CCWGG 2 cut(s) 61, 578
MwoI GCNNNNNNNGC 3 cut(s) 324, 725, 734
NcoI CCATGG 1 cut(s) 647
NdeII GATC 4 cut(s) 78, 450, 528, 687
NlaIII CATG 5 cut(s) 155, 208, 493, 596, 651
NlaIV GGNNCC 1 cut(s) 272
NspI RCATGY 1 cut(s) 155
PagI TCATGA 1 cut(s) 592
PciI ACATGT 1 cut(s) 151
PdmI GAANNNNTTC 1 cut(s) 620
PfoI TCCNGGA 1 cut(s) 576
PinAI ACCGGT 1 cut(s) 96
PkrI GCNGC 2 cut(s) 718, 736
PleI GAGTC 2 cut(s) 307, 663
PmeI GTTTAAAC 1 cut(s) 310
PpsI GAGTC 2 cut(s) 307, 663
PscI ACATGT 1 cut(s) 151
Psp6I CCWGG 2 cut(s) 59, 576
PspGI CCWGG 2 cut(s) 59, 576
PspN4I GGNNCC 1 cut(s) 272
PspPI GGNCC 1 cut(s) 47
RsaI GTAC 2 cut(s) 333, 382
RsaNI GTAC 2 cut(s) 332, 381
RseI CAYNNNNRTG 1 cut(s) 140
SaqAI TTAA 4 cut(s) 309, 363, 465, 678
SatI GCNGC 2 cut(s) 717, 735
Sau3AI GATC 4 cut(s) 78, 450, 528, 687
Sau96I GGNCC 1 cut(s) 47
SchI GAGTC 2 cut(s) 308, 664
ScrFI CCNGG 2 cut(s) 61, 578
SfaNI GCATC 3 cut(s) 132, 571, 737
SinI GGWCC 1 cut(s) 47
SmiMI CAYNNNNRTG 1 cut(s) 140
Sse9I AATT 1 cut(s) 238
SsiI CCGC 5 cut(s) 274, 325, 388, 732, 735
SspMI CTAG 1 cut(s) 437
StyD4I CCNGG 2 cut(s) 59, 576
StyI CCWWGG 2 cut(s) 647, 663
TaaI ACNGT 3 cut(s) 122, 315, 336
TaiI ACGT 1 cut(s) 576
TaqI TCGA 2 cut(s) 686, 693
TasI AATT 1 cut(s) 238
TatI WGTACW 1 cut(s) 331
TauI GCSGC 1 cut(s) 737
Tru1I TTAA 4 cut(s) 309, 363, 465, 678
Tru9I TTAA 4 cut(s) 309, 363, 465, 678
TseI GCWGC 1 cut(s) 716
TspDTI ATGAA 3 cut(s) 193, 309, 451
VpaK11BI GGWCC 1 cut(s) 47
XapI RAATTY 1 cut(s) 238
XceI RCATGY 1 cut(s) 155
XcmI CCANNNNNNNNNTGG 1 cut(s) 584
XmnI GAANNNNTTC 1 cut(s) 620
XspI CTAG 1 cut(s) 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.