pycom07g20470

Belongs to the eukaryotic ribosomal protein eS4 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001275
Physical Location & Seq
Forward (+)
22543679 .. 22546182
2504 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g20470.1

Sequence Viewer

Length: 972 bp
ATGATCCACGACTTGGATCCCCTCAAAGCCCACCCTGCGCCATTCTGTAGGGTTTTAGTGAGGGCGTCTCTCTCTCTCCCTCTCTCCCTCTCTCTCGCTCTCTCCCTCCCTCTTGCCCGTTCAGAGCTCCATCGGAAGCTCAGATACTTCCCCAGAGTTCAAGATCACAGTCCCAAAATGGCGAGAGGTTTGAAGAAGCATTTGAAGAGGCTCAATGCCCCGAAGCATTGGATGCTCGACAAACTTGGCGGAGCATTTGCACCTAAGCCTTCATCTGGGCCTCACAAGTCCAGGGAATGCCTTCCCTTGGTCATTATTCTACGAAACCGATTGAAATATGCTCTAACTTACCGGGAGGTCATTTCCATCTTGATGCAACGGCATGTTCTGGTTGACGGCAAGGTTAGGACTGACAAAACATATCCTTCCGGTTTCATGGATGTTATTTCAATCCCCAAAACAAATGAGAATTTCCGTCTCCTTTATGACACAAAGGGTCGGTTCCGTCTCCACTCAATCCGGGACGAAGAGGCAAAGTTCAAACTTTGCAAGGTCCGTTCTGTGCAATTTGGGCAGAAGGGCATCCCATACATTAACACCTACGACGGACGGACCATCCGCTACCCTGACCCTCTCATCAAGGCCAACGACACTATAAAGCTTGACCTAGAGGCCAACAAGATCACCGACTTCATTAAGTTCGATGTTGGCAATGTGGTGATGGTTACCGGTGGAAGGAACAGAGGGCGTGTTGGAGTAATCAAGAACAGGGAGAAACACAAGGGAAGTTTCGAGACAATCCACGTCCAGGATGCCACCGGCCATGAGTTTGCTACTCGATTGGGCAATGTGTTCACCATTGGCAAGGGGACAAAGCCGTGGGTAAGTCTTCCCAAGGGAAGGGGTATCAAGCTGACCATCATTGAAGAGGCGAAAAAGAGGCAAGCAGCCCAACAGGCAGCGACGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
40S_S4_C KOW RS4NT Ribosomal_S4e S4

Protein Analysis

324

Amino Acids

36.61

Weight (kDa)

10.34

Isoelectric Point (pI)

28.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RS4NT PF08071 62 - 98 3.1e-18 RS4NT (NUC023) domain
Ribosomal_S4e PF00900 154 - 228 8.5e-34 Ribosomal family S4e
KOW PF00467 236 - 269 2.5e-06 KOW motif
40S_S4_C PF16121 271 - 317 4.1e-25 40S ribosomal protein S4 C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 13
AciI CCGC 2 cut(s) 249, 619
AclWI GGATC 2 cut(s) 11, 24
AcoI YGGCCR 1 cut(s) 820
AcsI RAATTY 1 cut(s) 469
AcyI GRCGYC 1 cut(s) 65
AfiI CCNNNNNNNGG 6 cut(s) 13, 275, 307, 735, 808, 900
AgeI ACCGGT 1 cut(s) 728
AgsI TTSAA 7 cut(s) 161, 193, 205, 334, 450, 541, 926
AjiI CACGTC 1 cut(s) 805
AjnI CCWGG 2 cut(s) 290, 807
AluBI AGCT 4 cut(s) 127, 139, 661, 913
AluI AGCT 4 cut(s) 127, 139, 661, 913
Alw21I GWGCWC 1 cut(s) 129
Alw26I GTCTC 4 cut(s) 72, 482, 512, 788
AlwI GGATC 2 cut(s) 11, 24
AoxI GGCC 4 cut(s) 278, 642, 672, 820
ApeKI GCWGC 2 cut(s) 947, 959
ApoI RAATTY 1 cut(s) 469
AsiGI ACCGGT 1 cut(s) 728
Asp700I GAANNNNTTC 1 cut(s) 300
AspLEI GCGC 1 cut(s) 40
AspS9I GGNCC 3 cut(s) 278, 553, 612
AsuC2I CCSGG 2 cut(s) 353, 521
AsuHPI GGTGA 3 cut(s) 676, 730, 847
AvaII GGWCC 2 cut(s) 553, 612
BamHI GGATCC 1 cut(s) 16
BanII GRGCYC 1 cut(s) 129
BarI GAAGNNNNNNTAC 2 cut(s) 128, 160
BbsI GAAGAC 1 cut(s) 881
Bbv12I GWGCWC 1 cut(s) 129
BbvI GCAGC 1 cut(s) 959
BccI CCATC 5 cut(s) 138, 374, 623, 715, 926
BceAI ACGGC 3 cut(s) 395, 412, 862
BciT130I CCWGG 2 cut(s) 292, 809
BcnI CCSGG 2 cut(s) 353, 521
BcoDI GTCTC 4 cut(s) 72, 482, 512, 788
BfaI CTAG 1 cut(s) 668
BfmI CTRYAG 1 cut(s) 46
BglI GCCNNNNNGGC 1 cut(s) 956
BisI GCNGC 2 cut(s) 948, 960
BlsI GCNGC 2 cut(s) 949, 961
Bme1390I CCNGG 4 cut(s) 292, 353, 521, 809
Bme18I GGWCC 2 cut(s) 553, 612
BmgBI CACGTC 1 cut(s) 805
BmgT120I GGNCC 3 cut(s) 278, 553, 612
BmiI GGNNCC 2 cut(s) 18, 503
BmrFI CCNGG 4 cut(s) 292, 353, 521, 809
BmsI GCATC 4 cut(s) 222, 363, 591, 802
BpiI GAAGAC 1 cut(s) 881
BplI GAGNNNNNCTC 2 cut(s) 52, 84
Bpu10I CCTNAGC 1 cut(s) 264
BpuMI CCSGG 2 cut(s) 353, 521
BsaHI GRCGYC 1 cut(s) 65
BsaJI CCNNGG 4 cut(s) 291, 306, 878, 894
BsaWI WCCGGW 2 cut(s) 428, 728
Bsc4I CCNNNNNNNGG 6 cut(s) 13, 275, 307, 735, 808, 900
Bse118I RCCGGY 2 cut(s) 728, 818
Bse3DI GCAATG 2 cut(s) 718, 853
BseBI CCWGG 2 cut(s) 292, 809
BseDI CCNNGG 4 cut(s) 291, 306, 878, 894
BseGI GGATG 5 cut(s) 237, 445, 582, 615, 817
BseLI CCNNNNNNNGG 6 cut(s) 13, 275, 307, 735, 808, 900
BseMI GCAATG 2 cut(s) 718, 853
BseMII CTCAG 1 cut(s) 154
BseXI GCAGC 1 cut(s) 959
BshFI GGCC 4 cut(s) 280, 644, 674, 822
BshTI ACCGGT 1 cut(s) 728
BsiHKAI GWGCWC 1 cut(s) 129
BsiSI CCGG 5 cut(s) 352, 429, 520, 729, 819
BslFI GGGAC 3 cut(s) 156, 536, 883
BslI CCNNNNNNNGG 6 cut(s) 13, 275, 307, 735, 808, 900
BsmAI GTCTC 4 cut(s) 72, 482, 512, 788
BsmBI CGTCTC 3 cut(s) 72, 482, 512
BsmFI GGGAC 3 cut(s) 156, 536, 883
BsmI GAATGC 1 cut(s) 302
BsnI GGCC 4 cut(s) 280, 644, 674, 822
Bsp1286I GDGCHC 1 cut(s) 129
Bsp143I GATC 4 cut(s) 3, 16, 163, 681
BspACI CCGC 2 cut(s) 249, 619
BspANI GGCC 4 cut(s) 280, 644, 674, 822
BspCNI CTCAG 1 cut(s) 153
BspLI GGNNCC 2 cut(s) 18, 503
BspPI GGATC 2 cut(s) 11, 24
BsrDI GCAATG 2 cut(s) 718, 853
BsrFI RCCGGY 2 cut(s) 728, 818
BssAI RCCGGY 2 cut(s) 728, 818
BssECI CCNNGG 4 cut(s) 291, 306, 878, 894
BssMI GATC 4 cut(s) 3, 16, 163, 681
BssNI GRCGYC 1 cut(s) 65
BssT1I CCWWGG 2 cut(s) 306, 894
Bst2UI CCWGG 2 cut(s) 292, 809
Bst4CI ACNGT 1 cut(s) 170
Bst6I CTCTTC 3 cut(s) 200, 522, 921
BstACI GRCGYC 1 cut(s) 65
BstAPI GCANNNNNTGC 1 cut(s) 232
BstC8I GCNNGC 1 cut(s) 945
BstDEI CTNAG 2 cut(s) 140, 264
BstDSI CCRYGG 1 cut(s) 878
BstEII GGTNACC 1 cut(s) 724
BstF5I GGATG 5 cut(s) 237, 445, 582, 615, 817
BstHHI GCGC 1 cut(s) 40
BstKTI GATC 4 cut(s) 6, 19, 166, 684
BstMAI GTCTC 4 cut(s) 72, 482, 512, 788
BstMBI GATC 4 cut(s) 3, 16, 163, 681
BstMWI GCNNNNNNNGC 5 cut(s) 35, 232, 571, 956, 965
BstNI CCWGG 2 cut(s) 292, 809
BstNSI RCATGY 1 cut(s) 386
BstPI GGTNACC 1 cut(s) 724
BstSCI CCNGG 4 cut(s) 290, 351, 519, 807
BstSFI CTRYAG 1 cut(s) 46
BstV1I GCAGC 1 cut(s) 959
BstV2I GAAGAC 1 cut(s) 881
BstX2I RGATCY 1 cut(s) 16
BstYI RGATCY 1 cut(s) 16
BsuRI GGCC 4 cut(s) 280, 644, 674, 822
BtgI CCRYGG 1 cut(s) 878
BtrI CACGTC 1 cut(s) 805
BtsCI GGATG 5 cut(s) 237, 445, 582, 615, 817
Cac8I GCNNGC 1 cut(s) 945
CfoI GCGC 1 cut(s) 40
Cfr10I RCCGGY 2 cut(s) 728, 818
Cfr13I GGNCC 3 cut(s) 278, 553, 612
CseI GACGC 1 cut(s) 54
CspAI ACCGGT 1 cut(s) 728
CviAII CATG 3 cut(s) 383, 436, 824
DdeI CTNAG 2 cut(s) 140, 264
DpnI GATC 4 cut(s) 5, 18, 165, 683
DpnII GATC 4 cut(s) 3, 16, 163, 681
EaeI YGGCCR 1 cut(s) 820
Eam1104I CTCTTC 3 cut(s) 200, 522, 921
EarI CTCTTC 3 cut(s) 200, 522, 921
EciI GGCGGA 1 cut(s) 264
Ecl136II GAGCTC 1 cut(s) 127
Eco130I CCWWGG 2 cut(s) 306, 894
Eco24I GRGCYC 1 cut(s) 129
Eco47I GGWCC 2 cut(s) 553, 612
Eco53kI GAGCTC 1 cut(s) 127
Eco91I GGTNACC 1 cut(s) 724
EcoICRI GAGCTC 1 cut(s) 127
EcoO65I GGTNACC 1 cut(s) 724
EcoRII CCWGG 2 cut(s) 290, 807
EcoT14I CCWWGG 2 cut(s) 306, 894
EcoT38I GRGCYC 1 cut(s) 129
ErhI CCWWGG 2 cut(s) 306, 894
Esp3I CGTCTC 3 cut(s) 72, 482, 512
FaeI CATG 3 cut(s) 386, 439, 827
FaiI YATR 9 cut(s) 339, 384, 421, 437, 486, 589, 656, 825, 970
FaqI GGGAC 3 cut(s) 156, 536, 883
FatI CATG 3 cut(s) 382, 435, 823
Fnu4HI GCNGC 2 cut(s) 948, 960
FokI GGATG 5 cut(s) 244, 452, 569, 602, 824
FriOI GRGCYC 1 cut(s) 129
Fsp4HI GCNGC 2 cut(s) 948, 960
FspBI CTAG 1 cut(s) 668
GlaI GCGC 1 cut(s) 39
GluI GCNGC 2 cut(s) 948, 960
HaeIII GGCC 4 cut(s) 280, 644, 674, 822
HapII CCGG 5 cut(s) 352, 429, 520, 729, 819
HgaI GACGC 1 cut(s) 54
HhaI GCGC 1 cut(s) 40
Hin1I GRCGYC 1 cut(s) 65
Hin1II CATG 3 cut(s) 386, 439, 827
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HincII GTYRAC 1 cut(s) 394
HindII GTYRAC 1 cut(s) 394
HindIII AAGCTT 1 cut(s) 659
HpaII CCGG 5 cut(s) 352, 429, 520, 729, 819
HphI GGTGA 3 cut(s) 676, 730, 847
Hpy166II GTNNAC 2 cut(s) 394, 855
Hpy188I TCNGA 3 cut(s) 124, 135, 143
Hpy188III TCNNGA 4 cut(s) 161, 370, 763, 793
Hpy8I GTNNAC 2 cut(s) 394, 855
Hpy99I CGWCG 2 cut(s) 608, 967
HpyAV CCTTC 6 cut(s) 279, 311, 435, 571, 729, 894
HpyCH4III ACNGT 1 cut(s) 170
HpyCH4IV ACGT 1 cut(s) 804
HpyCH4V TGCA 4 cut(s) 260, 376, 549, 565
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 232, 571, 956, 965
HpyF3I CTNAG 2 cut(s) 140, 264
HpySE526I ACGT 1 cut(s) 804
Hsp92I GRCGYC 1 cut(s) 65
Hsp92II CATG 3 cut(s) 386, 439, 827
HspAI GCGC 1 cut(s) 38
Kzo9I GATC 4 cut(s) 3, 16, 163, 681
LmnI GCTCC 2 cut(s) 132, 251
Lsp1109I GCAGC 1 cut(s) 959
LweI GCATC 4 cut(s) 222, 363, 591, 802
MaeI CTAG 1 cut(s) 668
MaeII ACGT 1 cut(s) 804
MaeIII GTNAC 1 cut(s) 724
MalI GATC 4 cut(s) 5, 18, 165, 683
MboI GATC 4 cut(s) 3, 16, 163, 681
MboII GAAGA 5 cut(s) 205, 217, 539, 881, 938
MflI RGATCY 1 cut(s) 16
MhlI GDGCHC 1 cut(s) 129
MluCI AATT 2 cut(s) 469, 566
MmeI TCCRAC 1 cut(s) 733
MroXI GAANNNNTTC 1 cut(s) 300
MseI TTAA 2 cut(s) 594, 696
MslI CAYNNNNRTG 1 cut(s) 371
MspI CCGG 5 cut(s) 352, 429, 520, 729, 819
MspR9I CCNGG 4 cut(s) 292, 353, 521, 809
Mva1269I GAATGC 1 cut(s) 302
MvaI CCWGG 2 cut(s) 292, 809
MwoI GCNNNNNNNGC 5 cut(s) 35, 232, 571, 956, 965
NciI CCSGG 2 cut(s) 353, 521
NdeII GATC 4 cut(s) 3, 16, 163, 681
NlaIII CATG 3 cut(s) 386, 439, 827
NlaIV GGNNCC 2 cut(s) 18, 503
NspI RCATGY 1 cut(s) 386
PctI GAATGC 1 cut(s) 302
PdmI GAANNNNTTC 1 cut(s) 300
PflMI CCANNNNNTGG 1 cut(s) 13
PfoI TCCNGGA 2 cut(s) 519, 807
PinAI ACCGGT 1 cut(s) 728
PkrI GCNGC 2 cut(s) 949, 961
Psp124BI GAGCTC 1 cut(s) 129
Psp6I CCWGG 2 cut(s) 290, 807
PspEI GGTNACC 1 cut(s) 724
PspGI CCWGG 2 cut(s) 290, 807
PspN4I GGNNCC 2 cut(s) 18, 503
PspPI GGNCC 3 cut(s) 278, 553, 612
PsuI RGATCY 1 cut(s) 16
RseI CAYNNNNRTG 1 cut(s) 371
SacI GAGCTC 1 cut(s) 129
SaqAI TTAA 2 cut(s) 594, 696
SatI GCNGC 2 cut(s) 948, 960
Sau3AI GATC 4 cut(s) 3, 16, 163, 681
Sau96I GGNCC 3 cut(s) 278, 553, 612
ScrFI CCNGG 4 cut(s) 292, 353, 521, 809
SduI GDGCHC 1 cut(s) 129
SfaNI GCATC 4 cut(s) 222, 363, 591, 802
SfcI CTRYAG 1 cut(s) 46
SinI GGWCC 2 cut(s) 553, 612
SmiMI CAYNNNNRTG 1 cut(s) 371
Sse9I AATT 2 cut(s) 469, 566
SsiI CCGC 2 cut(s) 249, 619
SspMI CTAG 1 cut(s) 668
SstI GAGCTC 1 cut(s) 129
StyD4I CCNGG 4 cut(s) 290, 351, 519, 807
StyI CCWWGG 2 cut(s) 306, 894
TaaI ACNGT 1 cut(s) 170
TaiI ACGT 1 cut(s) 807
TaqI TCGA 4 cut(s) 237, 702, 792, 838
TasI AATT 2 cut(s) 469, 566
Tru1I TTAA 2 cut(s) 594, 696
Tru9I TTAA 2 cut(s) 594, 696
TseI GCWGC 2 cut(s) 947, 959
TspDTI ATGAA 3 cut(s) 261, 424, 682
TspGWI ACGGA 5 cut(s) 464, 494, 545, 621, 625
Van91I CCANNNNNTGG 1 cut(s) 13
VpaK11BI GGWCC 2 cut(s) 553, 612
XapI RAATTY 1 cut(s) 469
XceI RCATGY 1 cut(s) 386
XmnI GAANNNNTTC 1 cut(s) 300
XspI CTAG 1 cut(s) 668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.