MD03G1060900.v1.1

Belongs to the eukaryotic ribosomal protein eS4 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001275
Physical Location & Seq
Reverse (-)
4930368 .. 4932639
2272 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1060900.v1.1.491

Sequence Viewer

Length: 798 bp
ATGGCTAGAGGACTGAAGAAGCATTTGAAGAGGCTCAATGCCCCAAAGCATTGGATGCTCGACAAGCTTGGTGGTACGTTTGCCCCCAAGCCATCATCTGGTCCCCACAAAGCCAGGGAGTGCCTTCCCCTGGTTCTTATTCTGAGGAACAGGTTGAAGTATGCACTCACGTACCGTGAGGTTGTTTCAATTCTCATGCAGCGCCATATTTTGGTTGACGGAAAGGTTAGGACTGACAAGACTTACCCTTCTGGCTTCATGGATGTTGTTTCGATCCCAAAAACAAATGAGAATTTCCGTCTGCTCTTTGACACCAAGGGTCGGTTTAGGCTCCACTCAATTAGAGATGAGGAGGCCAAGTTCAAGCTCTGCAAGGTTCGATCAGTGCAGTTTGGGCAGAAGGGCATCCCCTATCTTAACACTTTTGATGGACGCACTATTCGCTACCCTGACCCGCTCATCAAGGCCAATGACACTATCAAGCTGGACTTAGAGGCCAACAAGATCACTGACTTCATTAAGTTTGATGTTGGTAATGTTGTCATGGTTACTGGCGGAAGGAATAGGGGGCGTGTTGGAGTCATTAAGAACAGGGAGAAGCATAAGGGAAGCTTTGAAACTATCCACGTACAGGATGCCCTTGGACATGAGTTTGCAACCCGTCTTGGTAATGTGTTCACCATCGGCAAGGGTACAAAGCCATGGGTGTCTCTTCCCAAGGGCAAGGGTATCAAGCTAAGCATCATTGAAGAGGCAAAGAAGCGACAAGCGGCCGCCCAAACAGCTGCTACTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
40S_S4_C KOW RS4NT Ribosomal_S4e S4

Protein Analysis

266

Amino Acids

29.9

Weight (kDa)

10.32

Isoelectric Point (pI)

23.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RS4NT PF08071 3 - 39 2.6e-19 RS4NT (NUC023) domain
Ribosomal_S4e PF00900 95 - 169 8.4e-34 Ribosomal family S4e
KOW PF00467 177 - 210 1.9e-06 KOW motif
40S_S4_C PF16121 212 - 258 1.3e-25 40S ribosomal protein S4 C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 98, 211
AccBSI CCGCTC 1 cut(s) 457
AciI CCGC 4 cut(s) 455, 555, 770, 774
AclWI GGATC 1 cut(s) 268
AcoI YGGCCR 1 cut(s) 771
AcsI RAATTY 1 cut(s) 292
AcuI CTGAAG 1 cut(s) 35
AfaI GTAC 4 cut(s) 76, 173, 630, 694
AfiI CCNNNNNNNGG 5 cut(s) 98, 130, 211, 321, 631
AgsI TTSAA 6 cut(s) 28, 157, 189, 364, 617, 749
AjnI CCWGG 2 cut(s) 113, 129
AloI GAACNNNNNNTCC 2 cut(s) 344, 376
AluBI AGCT 6 cut(s) 67, 367, 484, 612, 736, 785
AluI AGCT 6 cut(s) 67, 367, 484, 612, 736, 785
Alw26I GTCTC 1 cut(s) 714
AlwI GGATC 1 cut(s) 268
AoxI GGCC 4 cut(s) 354, 465, 495, 771
ApeKI GCWGC 2 cut(s) 199, 785
ApoI RAATTY 1 cut(s) 292
ArsI GACNNNNNNTTYG 2 cut(s) 423, 455
AspLEI GCGC 1 cut(s) 204
AspS9I GGNCC 1 cut(s) 101
AsuHPI GGTGA 1 cut(s) 670
AvaII GGWCC 1 cut(s) 101
BaeI ACNNNNGTAYC 2 cut(s) 155, 188
BbvI GCAGC 2 cut(s) 211, 772
BccI CCATC 3 cut(s) 100, 422, 689
BciT130I CCWGG 2 cut(s) 115, 131
BcoDI GTCTC 1 cut(s) 714
BfaI CTAG 1 cut(s) 6
BfoI RGCGCY 1 cut(s) 205
BisI GCNGC 4 cut(s) 200, 771, 774, 786
BlpI GCTNAGC 1 cut(s) 737
BlsI GCNGC 4 cut(s) 201, 772, 775, 787
Bme1390I CCNGG 2 cut(s) 115, 131
Bme18I GGWCC 1 cut(s) 101
BmgT120I GGNCC 1 cut(s) 101
BmiI GGNNCC 2 cut(s) 103, 332
BmrFI CCNGG 2 cut(s) 115, 131
BmsI GCATC 4 cut(s) 45, 414, 625, 750
Bpu1102I GCTNAGC 1 cut(s) 737
BsaAI YACGTR 2 cut(s) 171, 628
BsaJI CCNNGG 6 cut(s) 114, 129, 315, 640, 701, 717
BsaXI ACNNNNNCTCC 2 cut(s) 344, 374
Bsc4I CCNNNNNNNGG 5 cut(s) 98, 130, 211, 321, 631
Bse1I ACTGG 1 cut(s) 556
BseBI CCWGG 2 cut(s) 115, 131
BseDI CCNNGG 6 cut(s) 114, 129, 315, 640, 701, 717
BseGI GGATG 4 cut(s) 60, 268, 405, 640
BseLI CCNNNNNNNGG 5 cut(s) 98, 130, 211, 321, 631
BseMII CTCAG 1 cut(s) 134
BseNI ACTGG 1 cut(s) 556
BseRI GAGGAG 1 cut(s) 365
BseX3I CGGCCG 1 cut(s) 771
BseXI GCAGC 2 cut(s) 211, 772
BsgI GTGCAG 1 cut(s) 407
Bsh1285I CGRYCG 1 cut(s) 774
BshFI GGCC 4 cut(s) 356, 467, 497, 773
BsiEI CGRYCG 1 cut(s) 774
BslFI GGGAC 1 cut(s) 87
BslI CCNNNNNNNGG 5 cut(s) 98, 130, 211, 321, 631
BsmAI GTCTC 1 cut(s) 714
BsmFI GGGAC 1 cut(s) 87
BsnI GGCC 4 cut(s) 356, 467, 497, 773
Bsp143I GATC 3 cut(s) 273, 380, 504
Bsp1720I GCTNAGC 1 cut(s) 737
Bsp19I CCATGG 1 cut(s) 701
BspACI CCGC 4 cut(s) 455, 555, 770, 774
BspANI GGCC 4 cut(s) 356, 467, 497, 773
BspCNI CTCAG 1 cut(s) 135
BspLI GGNNCC 2 cut(s) 103, 332
BspPI GGATC 1 cut(s) 268
BsrBI CCGCTC 1 cut(s) 457
BsrI ACTGG 1 cut(s) 556
BssECI CCNNGG 6 cut(s) 114, 129, 315, 640, 701, 717
BssMI GATC 3 cut(s) 273, 380, 504
BssT1I CCWWGG 4 cut(s) 315, 640, 701, 717
Bst2UI CCWGG 2 cut(s) 115, 131
Bst4CI ACNGT 1 cut(s) 176
Bst6I CTCTTC 3 cut(s) 23, 717, 744
BstAPI GCANNNNNTGC 1 cut(s) 55
BstBAI YACGTR 2 cut(s) 171, 628
BstDEI CTNAG 3 cut(s) 143, 490, 737
BstDSI CCRYGG 1 cut(s) 701
BstF5I GGATG 4 cut(s) 60, 268, 405, 640
BstH2I RGCGCY 1 cut(s) 205
BstHHI GCGC 1 cut(s) 204
BstKTI GATC 3 cut(s) 276, 383, 507
BstMAI GTCTC 1 cut(s) 714
BstMBI GATC 3 cut(s) 273, 380, 504
BstMCI CGRYCG 1 cut(s) 774
BstMWI GCNNNNNNNGC 6 cut(s) 55, 64, 394, 441, 782, 791
BstNI CCWGG 2 cut(s) 115, 131
BstSCI CCNGG 2 cut(s) 113, 129
BstV1I GCAGC 2 cut(s) 211, 772
BstXI CCANNNNNNTGG 1 cut(s) 51
BstZI CGGCCG 1 cut(s) 771
BsuRI GGCC 4 cut(s) 356, 467, 497, 773
BtgI CCRYGG 1 cut(s) 701
BtsCI GGATG 4 cut(s) 60, 268, 405, 640
BtsIMutI CAGTG 2 cut(s) 390, 507
CciNI GCGGCCGC 1 cut(s) 771
CfoI GCGC 1 cut(s) 204
Cfr13I GGNCC 1 cut(s) 101
CseI GACGC 1 cut(s) 441
Csp6I GTAC 4 cut(s) 75, 172, 629, 693
CspCI CAANNNNNGTGG 2 cut(s) 52, 87
CviAII CATG 5 cut(s) 196, 259, 544, 647, 702
CviQI GTAC 4 cut(s) 75, 172, 629, 693
DdeI CTNAG 3 cut(s) 143, 490, 737
DpnI GATC 3 cut(s) 275, 382, 506
DpnII GATC 3 cut(s) 273, 380, 504
EaeI YGGCCR 1 cut(s) 771
EagI CGGCCG 1 cut(s) 771
Eam1104I CTCTTC 3 cut(s) 23, 717, 744
EarI CTCTTC 3 cut(s) 23, 717, 744
EciI GGCGGA 1 cut(s) 570
EclXI CGGCCG 1 cut(s) 771
Eco130I CCWWGG 4 cut(s) 315, 640, 701, 717
Eco47I GGWCC 1 cut(s) 101
Eco52I CGGCCG 1 cut(s) 771
Eco57I CTGAAG 1 cut(s) 35
EcoRII CCWGG 2 cut(s) 113, 129
EcoT14I CCWWGG 4 cut(s) 315, 640, 701, 717
ErhI CCWWGG 4 cut(s) 315, 640, 701, 717
FaeI CATG 5 cut(s) 199, 262, 547, 650, 705
FaiI YATR 8 cut(s) 162, 197, 207, 260, 545, 603, 648, 703
FalI AAGNNNNNCTT 4 cut(s) 473, 505, 596, 628
FaqI GGGAC 1 cut(s) 87
FatI CATG 5 cut(s) 195, 258, 543, 646, 701
FauI CCCGC 1 cut(s) 462
Fnu4HI GCNGC 4 cut(s) 200, 771, 774, 786
FokI GGATG 4 cut(s) 67, 275, 392, 647
Fsp4HI GCNGC 4 cut(s) 200, 771, 774, 786
FspBI CTAG 1 cut(s) 6
GlaI GCGC 1 cut(s) 203
GluI GCNGC 4 cut(s) 200, 771, 774, 786
HaeII RGCGCY 1 cut(s) 205
HaeIII GGCC 4 cut(s) 356, 467, 497, 773
HgaI GACGC 1 cut(s) 441
HhaI GCGC 1 cut(s) 204
Hin1II CATG 5 cut(s) 199, 262, 547, 650, 705
Hin6I GCGC 1 cut(s) 202
HinP1I GCGC 1 cut(s) 202
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HindIII AAGCTT 2 cut(s) 65, 610
HinfI GANTC 1 cut(s) 579
HphI GGTGA 1 cut(s) 670
Hpy166II GTNNAC 2 cut(s) 217, 678
Hpy188I TCNGA 1 cut(s) 144
Hpy8I GTNNAC 2 cut(s) 217, 678
HpyAV CCTTC 4 cut(s) 134, 258, 394, 552
HpyCH4III ACNGT 1 cut(s) 176
HpyCH4IV ACGT 3 cut(s) 77, 170, 627
HpyCH4V TGCA 5 cut(s) 164, 199, 372, 388, 656
HpyF10VI GCNNNNNNNGC 6 cut(s) 55, 64, 394, 441, 782, 791
HpyF3I CTNAG 3 cut(s) 143, 490, 737
HpySE526I ACGT 3 cut(s) 77, 170, 627
Hsp92II CATG 5 cut(s) 199, 262, 547, 650, 705
HspAI GCGC 1 cut(s) 202
Kzo9I GATC 3 cut(s) 273, 380, 504
LmnI GCTCC 1 cut(s) 336
Lsp1109I GCAGC 2 cut(s) 211, 772
LweI GCATC 4 cut(s) 45, 414, 625, 750
MaeI CTAG 1 cut(s) 6
MaeII ACGT 3 cut(s) 77, 170, 627
MaeIII GTNAC 1 cut(s) 547
MalI GATC 3 cut(s) 275, 382, 506
MbiI CCGCTC 1 cut(s) 457
MboI GATC 3 cut(s) 273, 380, 504
MboII GAAGA 4 cut(s) 28, 40, 704, 761
MluCI AATT 3 cut(s) 189, 292, 339
MlyI GAGTC 1 cut(s) 588
MmeI TCCRAC 1 cut(s) 556
MnlI CCTC 7 cut(s) 24, 138, 172, 343, 346, 487, 745
MseI TTAA 4 cut(s) 417, 519, 585, 796
MspA1I CMGCKG 1 cut(s) 785
MspR9I CCNGG 2 cut(s) 115, 131
MvaI CCWGG 2 cut(s) 115, 131
MwoI GCNNNNNNNGC 6 cut(s) 55, 64, 394, 441, 782, 791
NcoI CCATGG 1 cut(s) 701
NdeII GATC 3 cut(s) 273, 380, 504
NlaIII CATG 5 cut(s) 199, 262, 547, 650, 705
NlaIV GGNNCC 2 cut(s) 103, 332
NotI GCGGCCGC 1 cut(s) 771
PflMI CCANNNNNTGG 2 cut(s) 98, 211
PkrI GCNGC 4 cut(s) 201, 772, 775, 787
PleI GAGTC 1 cut(s) 587
PpsI GAGTC 1 cut(s) 587
Ppu21I YACGTR 2 cut(s) 171, 628
Psp6I CCWGG 2 cut(s) 113, 129
PspGI CCWGG 2 cut(s) 113, 129
PspN4I GGNNCC 2 cut(s) 103, 332
PspPI GGNCC 1 cut(s) 101
PvuII CAGCTG 1 cut(s) 785
RsaI GTAC 4 cut(s) 76, 173, 630, 694
RsaNI GTAC 4 cut(s) 75, 172, 629, 693
SaqAI TTAA 4 cut(s) 417, 519, 585, 796
SatI GCNGC 4 cut(s) 200, 771, 774, 786
Sau3AI GATC 3 cut(s) 273, 380, 504
Sau96I GGNCC 1 cut(s) 101
SchI GAGTC 1 cut(s) 588
ScrFI CCNGG 2 cut(s) 115, 131
SfaNI GCATC 4 cut(s) 45, 414, 625, 750
SinI GGWCC 1 cut(s) 101
Sse9I AATT 3 cut(s) 189, 292, 339
SsiI CCGC 4 cut(s) 455, 555, 770, 774
SspMI CTAG 1 cut(s) 6
StyD4I CCNGG 2 cut(s) 113, 129
StyI CCWWGG 4 cut(s) 315, 640, 701, 717
TaaI ACNGT 1 cut(s) 176
TaiI ACGT 3 cut(s) 80, 173, 630
TaqI TCGA 3 cut(s) 60, 272, 379
TasI AATT 3 cut(s) 189, 292, 339
TauI GCSGC 2 cut(s) 773, 776
Tru1I TTAA 4 cut(s) 417, 519, 585, 796
Tru9I TTAA 4 cut(s) 417, 519, 585, 796
TscAI CASTG 2 cut(s) 390, 514
TseI GCWGC 2 cut(s) 199, 785
TspDTI ATGAA 2 cut(s) 247, 505
TspGWI ACGGA 2 cut(s) 234, 287
TspRI CASTG 2 cut(s) 390, 514
Van91I CCANNNNNTGG 2 cut(s) 98, 211
VpaK11BI GGWCC 1 cut(s) 101
XapI RAATTY 1 cut(s) 292
XspI CTAG 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.