FvH4_3g19770

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0004635
Physical Location & Seq
Forward (+)
12931829 .. 12933297
1469 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g19770.t1

Sequence Viewer

Length: 747 bp
ATGGAGTTTGGGAGATTTCTGTGCCTCTTGTTCTTGGTTTGCTTGACGTGTTTTCTCCCTGCAACAGAAGGTTACAAGTTTTACGTTGGTGGAAGAGATGGGTGGGTTTTGAAACCATCTGAGAGTTACAATCACTGGGCTGAGAGGAATAGGTTCCAAGTCAATGACACTCTCTATTTCAAGTACAACAAAGGATCGGACTCGGTTCTGGTGGTGAACAAGGACAACTTCTTCAACTGCAACACAAGCAACCCCATTCAGAAACTAGACGAGGGCAACTCCGAGTTCAAGTTTGATAGGTCTGGACCGTTCTACTTCATCAGTGGCCAAGATGGTAACTGCGCCAATGGTCAGAAGCTGATCATCATCGTTCTCGCCGTCAGGCAGCGTCAAACTCCTCCTTCGGCTGCGCCACCACAAGGCTCAGCCCCAGCTCCAGCTCCAGCAACTGCTCCATCTTCATCATCATCACCCTCACATGCCCCAAGTACTAACCCAGTAACATCTCCAGTCCCAGCTCCGGCAGCCACTACTACTCCAACCTCGTCTCCTCCTAGCTCTGTCGGGACCGCACCAACACCGTCCGGAGATACTCCGTCCGGGGACGTGATGTCACCACCACCGGGGTCGAACCAGAACTCAACAGCACCGAAAGGAAACGGAGCGTATGGTGTAACGGCATCGAGTGTGTTGGTGTCTTCAGTTGTTGTTTTGGTCAGTTTGGCTTTTGTGAGCAGCTTTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

249

Amino Acids

26.11

Weight (kDa)

7.6

Isoelectric Point (pI)

55.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 35 - 118 7.3e-22 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 584
AciI CCGC 1 cut(s) 570
AclWI GGATC 1 cut(s) 202
AcoI YGGCCR 1 cut(s) 325
AcuI CTGAAG 1 cut(s) 684
AfaI GTAC 2 cut(s) 185, 490
AfiI CCNNNNNNNGG 3 cut(s) 419, 520, 623
AflIII ACRYGT 1 cut(s) 47
AgsI TTSAA 4 cut(s) 112, 181, 235, 289
AhdI GACNNNNNGTC 1 cut(s) 610
AjiI CACGTC 2 cut(s) 48, 607
AluBI AGCT 6 cut(s) 358, 434, 440, 518, 558, 738
AluI AGCT 6 cut(s) 358, 434, 440, 518, 558, 738
Alw26I GTCTC 1 cut(s) 552
AlwI GGATC 1 cut(s) 202
AlwNI CAGNNNCTG 2 cut(s) 358, 449
Aor13HI TCCGGA 1 cut(s) 584
AoxI GGCC 1 cut(s) 325
ApeKI GCWGC 4 cut(s) 385, 407, 524, 735
Asp700I GAANNNNTTC 1 cut(s) 152
AspLEI GCGC 2 cut(s) 344, 412
AspS9I GGNCC 2 cut(s) 305, 567
AsuC2I CCSGG 2 cut(s) 601, 624
AsuHPI GGTGA 3 cut(s) 226, 462, 606
AvaII GGWCC 2 cut(s) 305, 567
BalI TGGCCA 1 cut(s) 327
BbsI GAAGAC 1 cut(s) 690
BbvI GCAGC 3 cut(s) 394, 397, 536
BccI CCATC 4 cut(s) 92, 124, 326, 463
BceAI ACGGC 2 cut(s) 362, 693
BclI TGATCA 1 cut(s) 360
BcnI CCSGG 2 cut(s) 601, 624
BcoDI GTCTC 1 cut(s) 552
BfaI CTAG 2 cut(s) 266, 555
BisI GCNGC 4 cut(s) 386, 408, 525, 736
BlpI GCTNAGC 1 cut(s) 424
BlsI GCNGC 4 cut(s) 387, 409, 526, 737
BmcAI AGTACT 1 cut(s) 490
Bme1390I CCNGG 2 cut(s) 601, 624
Bme18I GGWCC 2 cut(s) 305, 567
BmeRI GACNNNNNGTC 1 cut(s) 610
BmgBI CACGTC 2 cut(s) 48, 607
BmgT120I GGNCC 2 cut(s) 305, 567
BmiI GGNNCC 2 cut(s) 155, 568
BmrFI CCNGG 2 cut(s) 601, 624
BmrI ACTGGG 2 cut(s) 145, 491
BmsI GCATC 1 cut(s) 689
BmuI ACTGGG 2 cut(s) 145, 491
BpiI GAAGAC 1 cut(s) 690
BplI GAGNNNNNCTC 2 cut(s) 263, 295
BpmI CTGGAG 3 cut(s) 420, 426, 492
Bpu1102I GCTNAGC 1 cut(s) 424
BpuMI CCSGG 2 cut(s) 601, 624
BsaBI GATNNNNATC 1 cut(s) 365
BsaJI CCNNGG 2 cut(s) 600, 623
BsaWI WCCGGW 1 cut(s) 584
BsaXI ACNNNNNCTCC 4 cut(s) 520, 532, 550, 562
Bsc4I CCNNNNNNNGG 3 cut(s) 419, 520, 623
Bse1I ACTGG 3 cut(s) 140, 497, 509
Bse8I GATNNNNATC 1 cut(s) 365
BseAI TCCGGA 1 cut(s) 584
BseDI CCNNGG 2 cut(s) 600, 623
BseJI GATNNNNATC 1 cut(s) 365
BseLI CCNNNNNNNGG 3 cut(s) 419, 520, 623
BseMII CTCAG 3 cut(s) 111, 132, 438
BseNI ACTGG 3 cut(s) 140, 497, 509
BseRI GAGGAG 2 cut(s) 387, 540
BseXI GCAGC 3 cut(s) 394, 397, 536
BseYI CCCAGC 2 cut(s) 430, 514
BshFI GGCC 1 cut(s) 327
BsiSI CCGG 4 cut(s) 521, 585, 600, 623
BslFI GGGAC 3 cut(s) 497, 580, 617
BslI CCNNNNNNNGG 3 cut(s) 419, 520, 623
BsmAI GTCTC 1 cut(s) 552
BsmBI CGTCTC 1 cut(s) 552
BsmFI GGGAC 3 cut(s) 497, 580, 617
BsnI GGCC 1 cut(s) 327
Bsp13I TCCGGA 1 cut(s) 584
Bsp143I GATC 2 cut(s) 194, 360
Bsp1720I GCTNAGC 1 cut(s) 424
BspACI CCGC 1 cut(s) 570
BspANI GGCC 1 cut(s) 327
BspCNI CTCAG 3 cut(s) 112, 133, 437
BspEI TCCGGA 1 cut(s) 584
BspLI GGNNCC 2 cut(s) 155, 568
BspPI GGATC 1 cut(s) 202
BsrI ACTGG 3 cut(s) 140, 497, 509
BssECI CCNNGG 2 cut(s) 600, 623
BssMI GATC 2 cut(s) 194, 360
Bst4CI ACNGT 2 cut(s) 309, 582
Bst6I CTCTTC 1 cut(s) 88
BstDEI CTNAG 3 cut(s) 120, 141, 424
BstHHI GCGC 2 cut(s) 344, 412
BstKTI GATC 2 cut(s) 197, 363
BstMAI GTCTC 1 cut(s) 552
BstMBI GATC 2 cut(s) 194, 360
BstMWI GCNNNNNNNGC 2 cut(s) 246, 524
BstNSI RCATGY 1 cut(s) 482
BstSCI CCNGG 2 cut(s) 599, 622
BstV1I GCAGC 3 cut(s) 394, 397, 536
BstV2I GAAGAC 1 cut(s) 690
BsuRI GGCC 1 cut(s) 327
BtrI CACGTC 2 cut(s) 48, 607
BtsIMutI CAGTG 2 cut(s) 133, 328
CaiI CAGNNNCTG 2 cut(s) 358, 449
CfoI GCGC 2 cut(s) 344, 412
Cfr13I GGNCC 2 cut(s) 305, 567
CseI GACGC 1 cut(s) 377
Csp6I GTAC 2 cut(s) 184, 489
CviAII CATG 1 cut(s) 479
CviQI GTAC 2 cut(s) 184, 489
DdeI CTNAG 3 cut(s) 120, 141, 424
DpnI GATC 2 cut(s) 196, 362
DpnII GATC 2 cut(s) 194, 360
DriI GACNNNNNGTC 1 cut(s) 610
EaeI YGGCCR 1 cut(s) 325
Eam1104I CTCTTC 1 cut(s) 88
Eam1105I GACNNNNNGTC 1 cut(s) 610
EarI CTCTTC 1 cut(s) 88
Eco47I GGWCC 2 cut(s) 305, 567
Eco57I CTGAAG 1 cut(s) 684
Esp3I CGTCTC 1 cut(s) 552
FaeI CATG 1 cut(s) 482
FaiI YATR 2 cut(s) 480, 669
FalI AAGNNNNNCTT 2 cut(s) 212, 244
FaqI GGGAC 3 cut(s) 497, 580, 617
FatI CATG 1 cut(s) 478
FbaI TGATCA 1 cut(s) 360
Fnu4HI GCNGC 4 cut(s) 386, 408, 525, 736
Fsp4HI GCNGC 4 cut(s) 386, 408, 525, 736
FspBI CTAG 2 cut(s) 266, 555
GlaI GCGC 2 cut(s) 343, 411
GluI GCNGC 4 cut(s) 386, 408, 525, 736
GsaI CCCAGC 2 cut(s) 434, 518
GsuI CTGGAG 3 cut(s) 420, 426, 492
HaeIII GGCC 1 cut(s) 327
HapII CCGG 4 cut(s) 521, 585, 600, 623
HgaI GACGC 1 cut(s) 377
HhaI GCGC 2 cut(s) 344, 412
Hin1II CATG 1 cut(s) 482
Hin6I GCGC 2 cut(s) 342, 410
HinP1I GCGC 2 cut(s) 342, 410
HinfI GANTC 1 cut(s) 200
HpaII CCGG 4 cut(s) 521, 585, 600, 623
HphI GGTGA 3 cut(s) 226, 462, 606
Hpy166II GTNNAC 1 cut(s) 217
Hpy188I TCNGA 5 cut(s) 121, 199, 261, 283, 354
Hpy188III TCNNGA 3 cut(s) 303, 565, 585
Hpy8I GTNNAC 1 cut(s) 217
HpyAV CCTTC 2 cut(s) 62, 411
HpyCH4III ACNGT 2 cut(s) 309, 582
HpyCH4IV ACGT 3 cut(s) 47, 84, 606
HpyCH4V TGCA 2 cut(s) 62, 240
HpyF10VI GCNNNNNNNGC 2 cut(s) 246, 524
HpyF3I CTNAG 3 cut(s) 120, 141, 424
HpySE526I ACGT 3 cut(s) 47, 84, 606
Hsp92II CATG 1 cut(s) 482
HspAI GCGC 2 cut(s) 342, 410
Kpn2I TCCGGA 1 cut(s) 584
Ksp22I TGATCA 1 cut(s) 360
Kzo9I GATC 2 cut(s) 194, 360
LmnI GCTCC 5 cut(s) 439, 445, 457, 523, 662
Lsp1109I GCAGC 3 cut(s) 394, 397, 536
LweI GCATC 1 cut(s) 689
MaeI CTAG 2 cut(s) 266, 555
MaeII ACGT 3 cut(s) 47, 84, 606
MaeIII GTNAC 6 cut(s) 71, 125, 335, 499, 612, 673
MalI GATC 2 cut(s) 196, 362
MboI GATC 2 cut(s) 194, 360
MboII GAAGA 4 cut(s) 105, 223, 450, 690
MlsI TGGCCA 1 cut(s) 327
MluNI TGGCCA 1 cut(s) 327
MlyI GAGTC 1 cut(s) 194
MmeI TCCRAC 1 cut(s) 563
MnlI CCTC 7 cut(s) 35, 138, 265, 408, 484, 553, 561
Mox20I TGGCCA 1 cut(s) 327
MroI TCCGGA 1 cut(s) 584
MroXI GAANNNNTTC 1 cut(s) 152
MscI TGGCCA 1 cut(s) 327
Msp20I TGGCCA 1 cut(s) 327
MspI CCGG 4 cut(s) 521, 585, 600, 623
MspR9I CCNGG 2 cut(s) 601, 624
MwoI GCNNNNNNNGC 2 cut(s) 246, 524
NciI CCSGG 2 cut(s) 601, 624
NdeII GATC 2 cut(s) 194, 360
NlaIII CATG 1 cut(s) 482
NlaIV GGNNCC 2 cut(s) 155, 568
NmuCI GTSAC 1 cut(s) 612
NspI RCATGY 1 cut(s) 482
PcsI WCGNNNNNNNCGW 1 cut(s) 375
PdmI GAANNNNTTC 1 cut(s) 152
PkrI GCNGC 4 cut(s) 387, 409, 526, 737
PleI GAGTC 1 cut(s) 194
PpsI GAGTC 1 cut(s) 194
PspFI CCCAGC 2 cut(s) 430, 514
PspN4I GGNNCC 2 cut(s) 155, 568
PspPI GGNCC 2 cut(s) 305, 567
PstNI CAGNNNCTG 2 cut(s) 358, 449
RsaI GTAC 2 cut(s) 185, 490
RsaNI GTAC 2 cut(s) 184, 489
SatI GCNGC 4 cut(s) 386, 408, 525, 736
Sau3AI GATC 2 cut(s) 194, 360
Sau96I GGNCC 2 cut(s) 305, 567
ScaI AGTACT 1 cut(s) 490
SchI GAGTC 1 cut(s) 194
ScrFI CCNGG 2 cut(s) 601, 624
SfaNI GCATC 1 cut(s) 689
SinI GGWCC 2 cut(s) 305, 567
SsiI CCGC 1 cut(s) 570
SspMI CTAG 2 cut(s) 266, 555
StyD4I CCNGG 2 cut(s) 599, 622
TaaI ACNGT 2 cut(s) 309, 582
TaiI ACGT 3 cut(s) 50, 87, 609
TaqI TCGA 2 cut(s) 629, 683
TatI WGTACW 2 cut(s) 183, 488
TscAI CASTG 2 cut(s) 140, 328
TseFI GTSAC 1 cut(s) 612
TseI GCWGC 4 cut(s) 385, 407, 524, 735
Tsp45I GTSAC 1 cut(s) 612
TspDTI ATGAA 2 cut(s) 307, 450
TspGWI ACGGA 2 cut(s) 585, 675
TspRI CASTG 2 cut(s) 140, 328
VpaK11BI GGWCC 2 cut(s) 305, 567
XceI RCATGY 1 cut(s) 482
XmnI GAANNNNTTC 1 cut(s) 152
XspI CTAG 2 cut(s) 266, 555
ZrmI AGTACT 1 cut(s) 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.