MD03G1229600.v1.1

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0004635
Physical Location & Seq
Reverse (-)
31487133 .. 31489223
2091 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1229600.v1.1.491

Sequence Viewer

Length: 1104 bp
ATGGAGTTTTGGAGATTTGTTTGGATTCAGATCTTGTTCTTTTCGCTGTGTTTGTGTTTGTGTTCAACCACACAAGGATACAAATTCTATGTTGGTGGGAGAGAGGGGTGGGTGTTGAACCCTTCTGAGAACTACAACCACTGGGCTGAGAGGAACAGGTTCAATGTCAATGACAATCTATATTTCAAGTACCAGAAAGGGTCAGACTCAGTGCTCGTCGTGAACAAAGACGACTACTTCAACTGCAACACCCAAAACCCTATTCAGAAACTCGACGGTGGCGACTCAGACTTCACCTTCGACAGGTCTGGACCTTTCTATTTCATCAGTGGTCAAAATGGTAATTGTGCAAAGGGTCAGAAGTTTCTAATTATTGTCCTCGCCCCGAGGCACCCCAAGCGACCAGTCCTCCCTCCTCCTCCTACTAGCCCTACCACCCCTACAGCTCCTGGCCCTAGCCCCACCGCCCCTACAGCTCCTGCCTATCCTCCCGCCACGTCTCCTCAGCAGCCCTACCAGCCTCCTGCGGCATCCCCACCACAAGGGCCAGGGCTACCACCAGCTGCGGGCCCAGTCTCTCCATCACCCTCACCTGTGATTACAAACCCTCCTCCATCTGCATCACCAGGCCCATGGTCATCACAACCGTCACCGTCGCCATCAACCTCTCCGGTACCAGCTGCTGGCCCATTCTCTCCATCACCATCACCTGTGATTACAAACCCTCCTCCAGCTGCCTCACCAGGCCCATGGTCATCACAACCGTCACCATCGCCGTCAACATATCCGGGGCCAGCTCCCTCAACGACAACATCTCCGGTGCCAGCTCCGTCGTCAGGCTATACTCCAAGTACTTCTCCGCCCTCACCGCCCCCGGAGGGGCTCGCACCTACCCCTTCTGGCGTGCCTGAGGGGCCCACACCTACTCCGTCCGACCAGCCTGCGGGCCCCACACCTCCGGGAACTGTTACTTCTCCGCCGCCAGGGTCCGAGGAGTCGACTGCGCCGAAGCCGTCAAATAATGGAGCGTTACGTGGTGTAACGGCTCCTTCGAGTGTGTTGGTGTCTTTGGCTGTTGTTCTGGTCAGTGTGGCTCTCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

368

Amino Acids

37.93

Weight (kDa)

6.71

Isoelectric Point (pI)

85.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 37 - 120 1.8e-21 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 673
AccB1I GGYRCC 3 cut(s) 390, 673, 820
AccB7I CCANNNNNTGG 1 cut(s) 683
AccI GTMKAC 1 cut(s) 998
AciI CCGC 9 cut(s) 465, 492, 527, 566, 860, 869, 944, 977, 980
AcsI RAATTY 1 cut(s) 83
AfaI GTAC 3 cut(s) 191, 675, 853
AfiI CCNNNNNNNGG 9 cut(s) 303, 542, 566, 683, 836, 878, 879, 943, 983
AgsI TTSAA 5 cut(s) 66, 118, 163, 187, 241
AjiI CACGTC 1 cut(s) 498
AjnI CCWGG 5 cut(s) 448, 547, 625, 742, 982
AjuI GAANNNNNNNTTGG 2 cut(s) 246, 278
AluBI AGCT 7 cut(s) 446, 476, 563, 680, 734, 797, 827
AluI AGCT 7 cut(s) 446, 476, 563, 680, 734, 797, 827
Alw21I GWGCWC 1 cut(s) 216
Alw26I GTCTC 2 cut(s) 504, 580
AlwNI CAGNNNCTG 3 cut(s) 449, 479, 683
Ama87I CYCGRG 1 cut(s) 385
AoxI GGCC 9 cut(s) 451, 545, 568, 628, 685, 745, 791, 914, 946
ApaI GGGCCC 3 cut(s) 572, 918, 950
ApeKI GCWGC 4 cut(s) 508, 563, 680, 734
ApoI RAATTY 1 cut(s) 83
ArsI GACNNNNNNTTYG 2 cut(s) 281, 313
Asp700I GAANNNNTTC 1 cut(s) 158
Asp718I GGTACC 1 cut(s) 673
AspLEI GCGC 1 cut(s) 1006
AsuC2I CCSGG 3 cut(s) 789, 875, 960
AvaI CYCGRG 1 cut(s) 385
AvaII GGWCC 2 cut(s) 311, 987
AxyI CCTNAGG 1 cut(s) 909
BaeGI GKGCMC 3 cut(s) 572, 918, 950
BanI GGYRCC 3 cut(s) 390, 673, 820
BanII GRGCYC 4 cut(s) 572, 885, 918, 950
Bbv12I GWGCWC 1 cut(s) 216
BbvCI CCTCAGC 1 cut(s) 504
BbvI GCAGC 4 cut(s) 520, 550, 667, 721
BccI CCATC 6 cut(s) 589, 622, 667, 706, 712, 778
BceAI ACGGC 3 cut(s) 760, 997, 1059
BcgI CGANNNNNNTGC 2 cut(s) 923, 957
BciT130I CCWGG 5 cut(s) 450, 549, 627, 744, 984
BciVI GTATCC 1 cut(s) 71
BcnI CCSGG 3 cut(s) 789, 875, 960
BcoDI GTCTC 2 cut(s) 504, 580
BfaI CTAG 2 cut(s) 426, 456
BfmI CTRYAG 2 cut(s) 441, 471
BfuI GTATCC 1 cut(s) 71
BglI GCCNNNNNGGC 1 cut(s) 913
BglII AGATCT 1 cut(s) 30
BisI GCNGC 6 cut(s) 509, 528, 564, 681, 735, 980
BlsI GCNGC 6 cut(s) 510, 529, 565, 682, 736, 981
BmcAI AGTACT 1 cut(s) 853
Bme1390I CCNGG 8 cut(s) 450, 549, 627, 744, 789, 875, 960, 984
Bme18I GGWCC 2 cut(s) 311, 987
BmeT110I CYCGRG 1 cut(s) 385
BmgBI CACGTC 1 cut(s) 498
BmrFI CCNGG 8 cut(s) 450, 549, 627, 744, 789, 875, 960, 984
BmrI ACTGGG 2 cut(s) 151, 566
BmsI GCATC 2 cut(s) 539, 629
BmuI ACTGGG 2 cut(s) 151, 566
BpmI CTGGAG 1 cut(s) 714
Bpu10I CCTNAGC 1 cut(s) 504
BpuMI CCSGG 3 cut(s) 789, 875, 960
BsaAI YACGTR 1 cut(s) 1034
BsaBI GATNNNNATC 1 cut(s) 29
BsaJI CCNNGG 8 cut(s) 386, 548, 632, 749, 788, 873, 983, 990
BsaWI WCCGGW 2 cut(s) 670, 817
Bsc4I CCNNNNNNNGG 9 cut(s) 303, 542, 566, 683, 836, 878, 879, 943, 983
Bse1I ACTGG 3 cut(s) 146, 404, 572
Bse21I CCTNAGG 1 cut(s) 909
Bse8I GATNNNNATC 1 cut(s) 29
BseBI CCWGG 5 cut(s) 450, 549, 627, 744, 984
BseDI CCNNGG 8 cut(s) 386, 548, 632, 749, 788, 873, 983, 990
BseGI GGATG 1 cut(s) 530
BseJI GATNNNNATC 1 cut(s) 29
BseLI CCNNNNNNNGG 9 cut(s) 303, 542, 566, 683, 836, 878, 879, 943, 983
BseMII CTCAG 6 cut(s) 117, 138, 222, 300, 518, 900
BseNI ACTGG 3 cut(s) 146, 404, 572
BseRI GAGGAG 6 cut(s) 405, 408, 492, 600, 717, 1007
BseSI GKGCMC 3 cut(s) 572, 918, 950
BseXI GCAGC 4 cut(s) 520, 550, 667, 721
BshFI GGCC 9 cut(s) 453, 547, 570, 630, 687, 747, 793, 916, 948
BshNI GGYRCC 3 cut(s) 390, 673, 820
BsiHKAI GWGCWC 1 cut(s) 216
BsiHKCI CYCGRG 1 cut(s) 385
BsiSI CCGG 5 cut(s) 671, 788, 818, 875, 959
BslI CCNNNNNNNGG 9 cut(s) 303, 542, 566, 683, 836, 878, 879, 943, 983
BsmAI GTCTC 2 cut(s) 504, 580
BsmBI CGTCTC 1 cut(s) 504
BsnI GGCC 9 cut(s) 453, 547, 570, 630, 687, 747, 793, 916, 948
BsoBI CYCGRG 1 cut(s) 385
Bsp120I GGGCCC 3 cut(s) 568, 914, 946
Bsp1286I GDGCHC 5 cut(s) 216, 572, 885, 918, 950
Bsp143I GATC 1 cut(s) 30
Bsp19I CCATGG 2 cut(s) 632, 749
BspACI CCGC 9 cut(s) 465, 492, 527, 566, 860, 869, 944, 977, 980
BspANI GGCC 9 cut(s) 453, 547, 570, 630, 687, 747, 793, 916, 948
BspCNI CTCAG 6 cut(s) 118, 139, 221, 299, 517, 901
BspHI TCATGA 1 cut(s) 1100
BspT107I GGYRCC 3 cut(s) 390, 673, 820
BsrI ACTGG 3 cut(s) 146, 404, 572
BssECI CCNNGG 8 cut(s) 386, 548, 632, 749, 788, 873, 983, 990
BssMI GATC 1 cut(s) 30
BssT1I CCWWGG 2 cut(s) 632, 749
Bst2UI CCWGG 5 cut(s) 450, 549, 627, 744, 984
Bst4CI ACNGT 5 cut(s) 278, 648, 654, 765, 967
BstBAI YACGTR 1 cut(s) 1034
BstC8I GCNNGC 8 cut(s) 568, 685, 795, 825, 885, 905, 942, 946
BstDEI CTNAG 6 cut(s) 126, 147, 208, 286, 504, 909
BstDSI CCRYGG 2 cut(s) 632, 749
BstENI CCTNNNNNAGG 1 cut(s) 301
BstF5I GGATG 1 cut(s) 530
BstHHI GCGC 1 cut(s) 1006
BstKTI GATC 1 cut(s) 33
BstMAI GTCTC 2 cut(s) 504, 580
BstMBI GATC 1 cut(s) 30
BstMWI GCNNNNNNNGC 5 cut(s) 397, 473, 517, 868, 913
BstNI CCWGG 5 cut(s) 450, 549, 627, 744, 984
BstSCI CCNGG 8 cut(s) 448, 547, 625, 742, 787, 873, 958, 982
BstSFI CTRYAG 2 cut(s) 441, 471
BstSLI GKGCMC 3 cut(s) 572, 918, 950
BstV1I GCAGC 4 cut(s) 520, 550, 667, 721
BstX2I RGATCY 1 cut(s) 30
BstXI CCANNNNNNTGG 2 cut(s) 633, 750
BstYI RGATCY 1 cut(s) 30
Bsu36I CCTNAGG 1 cut(s) 909
BsuI GTATCC 1 cut(s) 71
BsuRI GGCC 9 cut(s) 453, 547, 570, 630, 687, 747, 793, 916, 948
BtgI CCRYGG 2 cut(s) 632, 749
BtgZI GCGATG 1 cut(s) 756
BtrI CACGTC 1 cut(s) 498
BtsCI GGATG 1 cut(s) 530
BtsIMutI CAGTG 4 cut(s) 139, 216, 334, 1093
Cac8I GCNNGC 8 cut(s) 568, 685, 795, 825, 885, 905, 942, 946
CaiI CAGNNNCTG 3 cut(s) 449, 479, 683
CciI TCATGA 1 cut(s) 1100
CfoI GCGC 1 cut(s) 1006
Csp6I GTAC 3 cut(s) 190, 674, 852
CviAII CATG 3 cut(s) 633, 750, 1101
CviQI GTAC 3 cut(s) 190, 674, 852
DdeI CTNAG 6 cut(s) 126, 147, 208, 286, 504, 909
DpnI GATC 1 cut(s) 32
DpnII GATC 1 cut(s) 30
EciI GGCGGA 2 cut(s) 849, 966
Eco130I CCWWGG 2 cut(s) 632, 749
Eco24I GRGCYC 4 cut(s) 572, 885, 918, 950
Eco47I GGWCC 2 cut(s) 311, 987
Eco81I CCTNAGG 1 cut(s) 909
Eco88I CYCGRG 1 cut(s) 385
EcoNI CCTNNNNNAGG 1 cut(s) 301
EcoO109I RGGNCCY 2 cut(s) 914, 947
EcoRII CCWGG 5 cut(s) 448, 547, 625, 742, 982
EcoT14I CCWWGG 2 cut(s) 632, 749
EcoT38I GRGCYC 4 cut(s) 572, 885, 918, 950
ErhI CCWWGG 2 cut(s) 632, 749
Esp3I CGTCTC 1 cut(s) 504
FaeI CATG 3 cut(s) 636, 753, 1104
FaiI YATR 7 cut(s) 90, 181, 634, 751, 784, 843, 1102
FatI CATG 3 cut(s) 632, 749, 1100
FauI CCCGC 3 cut(s) 499, 559, 937
FblI GTMKAC 1 cut(s) 998
Fnu4HI GCNGC 6 cut(s) 509, 528, 564, 681, 735, 980
FokI GGATG 1 cut(s) 517
FriOI GRGCYC 4 cut(s) 572, 885, 918, 950
Fsp4HI GCNGC 6 cut(s) 509, 528, 564, 681, 735, 980
FspBI CTAG 2 cut(s) 426, 456
GlaI GCGC 1 cut(s) 1005
GluI GCNGC 6 cut(s) 509, 528, 564, 681, 735, 980
GsuI CTGGAG 1 cut(s) 714
HaeIII GGCC 9 cut(s) 453, 547, 570, 630, 687, 747, 793, 916, 948
HapII CCGG 5 cut(s) 671, 788, 818, 875, 959
HhaI GCGC 1 cut(s) 1006
Hin1II CATG 3 cut(s) 636, 753, 1104
Hin6I GCGC 1 cut(s) 1004
HinP1I GCGC 1 cut(s) 1004
HincII GTYRAC 2 cut(s) 780, 999
HindII GTYRAC 2 cut(s) 780, 999
HinfI GANTC 4 cut(s) 25, 206, 284, 995
HpaII CCGG 5 cut(s) 671, 788, 818, 875, 959
Hpy166II GTNNAC 3 cut(s) 223, 780, 999
Hpy188I TCNGA 8 cut(s) 30, 127, 205, 267, 289, 360, 934, 991
Hpy188III TCNNGA 3 cut(s) 220, 309, 1101
Hpy8I GTNNAC 3 cut(s) 223, 780, 999
Hpy99I CGWCG 4 cut(s) 221, 278, 658, 835
HpyAV CCTTC 4 cut(s) 132, 307, 906, 1059
HpyCH4III ACNGT 5 cut(s) 278, 648, 654, 765, 967
HpyCH4IV ACGT 2 cut(s) 497, 1033
HpyCH4V TGCA 3 cut(s) 246, 350, 620
HpyF10VI GCNNNNNNNGC 5 cut(s) 397, 473, 517, 868, 913
HpyF3I CTNAG 6 cut(s) 126, 147, 208, 286, 504, 909
HpySE526I ACGT 2 cut(s) 497, 1033
Hsp92II CATG 3 cut(s) 636, 753, 1104
HspAI GCGC 1 cut(s) 1004
KpnI GGTACC 1 cut(s) 677
Kzo9I GATC 1 cut(s) 30
LmnI GCTCC 6 cut(s) 451, 481, 802, 832, 1025, 1051
Lsp1109I GCAGC 4 cut(s) 520, 550, 667, 721
LweI GCATC 2 cut(s) 539, 629
MaeI CTAG 2 cut(s) 426, 456
MaeII ACGT 2 cut(s) 497, 1033
MaeIII GTNAC 5 cut(s) 648, 765, 967, 1029, 1039
MalI GATC 1 cut(s) 32
MboI GATC 1 cut(s) 30
MflI RGATCY 1 cut(s) 30
MhlI GDGCHC 5 cut(s) 216, 572, 885, 918, 950
MluCI AATT 3 cut(s) 83, 343, 369
MlyI GAGTC 3 cut(s) 200, 278, 1004
MmeI TCCRAC 1 cut(s) 957
MroXI GAANNNNTTC 1 cut(s) 158
MspA1I CMGCKG 3 cut(s) 563, 680, 734
MspI CCGG 5 cut(s) 671, 788, 818, 875, 959
MspR9I CCNGG 8 cut(s) 450, 549, 627, 744, 789, 875, 960, 984
MvaI CCWGG 5 cut(s) 450, 549, 627, 744, 984
MwoI GCNNNNNNNGC 5 cut(s) 397, 473, 517, 868, 913
NciI CCSGG 3 cut(s) 789, 875, 960
NcoI CCATGG 2 cut(s) 632, 749
NdeII GATC 1 cut(s) 30
NlaIII CATG 3 cut(s) 636, 753, 1104
NmuCI GTSAC 2 cut(s) 648, 765
PagI TCATGA 1 cut(s) 1100
PcsI WCGNNNNNNNCGW 3 cut(s) 279, 1004, 1049
PdmI GAANNNNTTC 1 cut(s) 158
PfeI GAWTC 1 cut(s) 25
PflMI CCANNNNNTGG 1 cut(s) 683
PfoI TCCNGGA 1 cut(s) 958
PkrI GCNGC 6 cut(s) 510, 529, 565, 682, 736, 981
PleI GAGTC 3 cut(s) 200, 278, 1003
PpsI GAGTC 3 cut(s) 200, 278, 1003
Ppu21I YACGTR 1 cut(s) 1034
Psp6I CCWGG 5 cut(s) 448, 547, 625, 742, 982
PspGI CCWGG 5 cut(s) 448, 547, 625, 742, 982
PspOMI GGGCCC 3 cut(s) 568, 914, 946
PstNI CAGNNNCTG 3 cut(s) 449, 479, 683
PsuI RGATCY 1 cut(s) 30
PvuII CAGCTG 3 cut(s) 563, 680, 734
RsaI GTAC 3 cut(s) 191, 675, 853
RsaNI GTAC 3 cut(s) 190, 674, 852
SalI GTCGAC 1 cut(s) 997
SatI GCNGC 6 cut(s) 509, 528, 564, 681, 735, 980
Sau3AI GATC 1 cut(s) 30
ScaI AGTACT 1 cut(s) 853
SchI GAGTC 3 cut(s) 200, 278, 1004
ScrFI CCNGG 8 cut(s) 450, 549, 627, 744, 789, 875, 960, 984
SduI GDGCHC 5 cut(s) 216, 572, 885, 918, 950
SfaNI GCATC 2 cut(s) 539, 629
SfcI CTRYAG 2 cut(s) 441, 471
SinI GGWCC 2 cut(s) 311, 987
Sse9I AATT 3 cut(s) 83, 343, 369
SsiI CCGC 9 cut(s) 465, 492, 527, 566, 860, 869, 944, 977, 980
SspMI CTAG 2 cut(s) 426, 456
StyD4I CCNGG 8 cut(s) 448, 547, 625, 742, 787, 873, 958, 982
StyI CCWWGG 2 cut(s) 632, 749
TaaI ACNGT 5 cut(s) 278, 648, 654, 765, 967
TaiI ACGT 2 cut(s) 500, 1036
TaqI TCGA 4 cut(s) 273, 300, 998, 1052
TasI AATT 3 cut(s) 83, 343, 369
TatI WGTACW 1 cut(s) 851
TauI GCSGC 2 cut(s) 530, 982
TfiI GAWTC 1 cut(s) 25
TscAI CASTG 4 cut(s) 146, 216, 334, 1093
TseFI GTSAC 2 cut(s) 648, 765
TseI GCWGC 4 cut(s) 508, 563, 680, 734
Tsp45I GTSAC 2 cut(s) 648, 765
TspDTI ATGAA 1 cut(s) 313
TspGWI ACGGA 2 cut(s) 819, 918
TspRI CASTG 4 cut(s) 146, 216, 334, 1093
Van91I CCANNNNNTGG 1 cut(s) 683
VpaK11BI GGWCC 2 cut(s) 311, 987
XagI CCTNNNNNAGG 1 cut(s) 301
XapI RAATTY 1 cut(s) 83
XmiI GTMKAC 1 cut(s) 998
XmnI GAANNNNTTC 1 cut(s) 158
XspI CTAG 2 cut(s) 426, 456
ZrmI AGTACT 1 cut(s) 853
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.