RchiOBHm_Chr7g0225651

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0004635
Physical Location & Seq
Reverse (-)
48490296 .. 48491097
802 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20210

Sequence Viewer

Length: 594 bp
ATGGGGTCTAAAAGATGTATGAGCGTTTTGGTTGTCGTTTTCATGGCGTTGGTCAGCTATTCTCAAGCACACAAGTTCTTTGTGGGTGGCAAAGATGGGTGGATAGTGAACCCGTCTGAGAACTTCAATCACTGGGCCGGAAGGCATAGGTTTCAGGTCAATGATACGCTCTGTTTCAAGTACAAAAAGGGCTCAAACTCTGTCTTGGTTGTAACAAAAGAAGACTACTACTCGTGCAACAACAACAACCCAATACAGTACTTAACAGACGGTGACTCCGTCTTCAGATTTGAACGCTCCGGTCCTTTTTTTTTCATCAGCGGCAATGCCGACGATTGCCAAAATGGTCAGAAACTGATAGTAGTAGTTTTGGCCCTAAGACACAAATCTGACCACGGCGATGCTCCACTGGTACCCTCTCCTTCTCCGGTGGCTTCTCCTAACTCTGATAGTAATCCATCGGATTTAAACAACAAACCAACTCCGCCACTGAAGGAGAATTCGGGGTCACTGCTGCGTTTTAAAGGATCAGATGTTGGGTTGGTATGTCTTGGTATTGTTACCAGTATGATTTTTAGTAACTTTAGGGTTTGA

Protein Analysis

197

Amino Acids

21.74

Weight (kDa)

8.81

Isoelectric Point (pI)

35.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 34 - 117 1e-20 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 412
AccB1I GGYRCC 1 cut(s) 412
AciI CCGC 2 cut(s) 321, 485
AclWI GGATC 1 cut(s) 535
AcsI RAATTY 1 cut(s) 499
AcuI CTGAAG 2 cut(s) 268, 512
AfaI GTAC 3 cut(s) 182, 260, 414
AgsI TTSAA 3 cut(s) 127, 178, 293
AluBI AGCT 1 cut(s) 57
AluI AGCT 1 cut(s) 57
AlwI GGATC 1 cut(s) 535
AlwNI CAGNNNCTG 1 cut(s) 355
AoxI GGCC 2 cut(s) 135, 372
ApeKI GCWGC 1 cut(s) 514
ApoI RAATTY 1 cut(s) 499
Asp718I GGTACC 1 cut(s) 412
AspS9I GGNCC 3 cut(s) 135, 302, 373
AsuHPI GGTGA 1 cut(s) 284
AvaII GGWCC 1 cut(s) 302
BanI GGYRCC 1 cut(s) 412
BanII GRGCYC 1 cut(s) 194
BauI CACGAG 1 cut(s) 232
BbsI GAAGAC 2 cut(s) 228, 274
BbvI GCAGC 1 cut(s) 501
BccI CCATC 2 cut(s) 89, 466
BceAI ACGGC 1 cut(s) 412
BisI GCNGC 2 cut(s) 322, 515
BlsI GCNGC 2 cut(s) 323, 516
BmcAI AGTACT 1 cut(s) 260
Bme18I GGWCC 1 cut(s) 302
BmgT120I GGNCC 3 cut(s) 135, 302, 373
BmiI GGNNCC 1 cut(s) 414
BmrI ACTGGG 1 cut(s) 142
BmsI GCATC 1 cut(s) 391
BmuI ACTGGG 1 cut(s) 142
BpiI GAAGAC 2 cut(s) 228, 274
BpuEI CTTGAG 1 cut(s) 48
BsaBI GATNNNNATC 1 cut(s) 453
BsaJI CCNNGG 1 cut(s) 394
BsaWI WCCGGW 2 cut(s) 299, 427
BsaXI ACNNNNNCTCC 2 cut(s) 260, 290
Bse1I ACTGG 3 cut(s) 137, 414, 564
Bse3DI GCAATG 1 cut(s) 331
Bse8I GATNNNNATC 1 cut(s) 453
BseDI CCNNGG 1 cut(s) 394
BseJI GATNNNNATC 1 cut(s) 453
BseMI GCAATG 1 cut(s) 331
BseMII CTCAG 1 cut(s) 108
BseNI ACTGG 3 cut(s) 137, 414, 564
BseXI GCAGC 1 cut(s) 501
BshFI GGCC 2 cut(s) 137, 374
BshNI GGYRCC 1 cut(s) 412
BsiSI CCGG 3 cut(s) 138, 300, 428
BsnI GGCC 2 cut(s) 137, 374
Bsp1286I GDGCHC 1 cut(s) 194
Bsp143I GATC 1 cut(s) 527
BspACI CCGC 2 cut(s) 321, 485
BspANI GGCC 2 cut(s) 137, 374
BspCNI CTCAG 1 cut(s) 109
BspLI GGNNCC 1 cut(s) 414
BspPI GGATC 1 cut(s) 535
BspT107I GGYRCC 1 cut(s) 412
BsrDI GCAATG 1 cut(s) 331
BsrI ACTGG 3 cut(s) 137, 414, 564
BssECI CCNNGG 1 cut(s) 394
BssMI GATC 1 cut(s) 527
BssSI CACGAG 1 cut(s) 232
Bst2BI CACGAG 1 cut(s) 232
Bst4CI ACNGT 2 cut(s) 258, 272
BstDEI CTNAG 2 cut(s) 117, 377
BstDSI CCRYGG 1 cut(s) 394
BstKTI GATC 1 cut(s) 530
BstMBI GATC 1 cut(s) 527
BstV1I GCAGC 1 cut(s) 501
BstV2I GAAGAC 2 cut(s) 228, 274
BsuRI GGCC 2 cut(s) 137, 374
BtgI CCRYGG 1 cut(s) 394
BtgZI GCGATG 1 cut(s) 414
BtsI GCAGTG 1 cut(s) 509
BtsIMutI CAGTG 4 cut(s) 130, 407, 488, 509
CaiI CAGNNNCTG 1 cut(s) 355
Cfr13I GGNCC 3 cut(s) 135, 302, 373
Csp6I GTAC 3 cut(s) 181, 259, 413
CviAII CATG 1 cut(s) 43
CviJI RGCY 5 cut(s) 57, 137, 192, 374, 434
CviKI_1 RGCY 5 cut(s) 57, 137, 192, 374, 434
CviQI GTAC 3 cut(s) 181, 259, 413
DdeI CTNAG 2 cut(s) 117, 377
DpnI GATC 1 cut(s) 529
DpnII GATC 1 cut(s) 527
DraI TTTAAA 2 cut(s) 468, 523
EciI GGCGGA 1 cut(s) 474
Eco24I GRGCYC 1 cut(s) 194
Eco47I GGWCC 1 cut(s) 302
Eco57I CTGAAG 2 cut(s) 268, 512
EcoRI GAATTC 1 cut(s) 499
EcoT38I GRGCYC 1 cut(s) 194
FaeI CATG 1 cut(s) 46
FaiI YATR 5 cut(s) 20, 44, 147, 547, 569
FatI CATG 1 cut(s) 42
Fnu4HI GCNGC 2 cut(s) 322, 515
FriOI GRGCYC 1 cut(s) 194
Fsp4HI GCNGC 2 cut(s) 322, 515
GluI GCNGC 2 cut(s) 322, 515
HaeIII GGCC 2 cut(s) 137, 374
HapII CCGG 3 cut(s) 138, 300, 428
Hin1II CATG 1 cut(s) 46
HinfI GANTC 1 cut(s) 275
HpaII CCGG 3 cut(s) 138, 300, 428
HphI GGTGA 1 cut(s) 284
Hpy166II GTNNAC 1 cut(s) 109
Hpy188I TCNGA 7 cut(s) 118, 287, 351, 391, 448, 463, 532
Hpy8I GTNNAC 1 cut(s) 109
Hpy99I CGWCG 1 cut(s) 335
HpyAV CCTTC 3 cut(s) 135, 432, 487
HpyCH4III ACNGT 2 cut(s) 258, 272
HpyCH4V TGCA 1 cut(s) 237
HpyF3I CTNAG 2 cut(s) 117, 377
Hsp92II CATG 1 cut(s) 46
KpnI GGTACC 1 cut(s) 416
Kzo9I GATC 1 cut(s) 527
LmnI GCTCC 2 cut(s) 302, 409
LpnPI CCDG 7 cut(s) 118, 140, 151, 313, 395, 441, 577
Lsp1109I GCAGC 1 cut(s) 501
LweI GCATC 1 cut(s) 391
MaeIII GTNAC 5 cut(s) 211, 272, 507, 559, 578
MalI GATC 1 cut(s) 529
MboI GATC 1 cut(s) 527
MboII GAAGA 2 cut(s) 233, 274
MhlI GDGCHC 1 cut(s) 194
MluCI AATT 1 cut(s) 499
MlyI GAGTC 1 cut(s) 269
MnlI CCTC 1 cut(s) 427
MseI TTAA 3 cut(s) 263, 467, 522
MslI CAYNNNNRTG 1 cut(s) 399
MspA1I CMGCKG 1 cut(s) 321
MspI CCGG 3 cut(s) 138, 300, 428
NdeII GATC 1 cut(s) 527
NlaIII CATG 1 cut(s) 46
NlaIV GGNNCC 1 cut(s) 414
NmuCI GTSAC 2 cut(s) 272, 507
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PflFI GACNNNGTC 1 cut(s) 278
PkrI GCNGC 2 cut(s) 323, 516
PleI GAGTC 1 cut(s) 269
PpsI GAGTC 1 cut(s) 269
PspN4I GGNNCC 1 cut(s) 414
PspPI GGNCC 3 cut(s) 135, 302, 373
PstNI CAGNNNCTG 1 cut(s) 355
PsyI GACNNNGTC 1 cut(s) 278
RsaI GTAC 3 cut(s) 182, 260, 414
RsaNI GTAC 3 cut(s) 181, 259, 413
RseI CAYNNNNRTG 1 cut(s) 399
SaqAI TTAA 3 cut(s) 263, 467, 522
SatI GCNGC 2 cut(s) 322, 515
Sau3AI GATC 1 cut(s) 527
Sau96I GGNCC 3 cut(s) 135, 302, 373
ScaI AGTACT 1 cut(s) 260
SchI GAGTC 1 cut(s) 269
SduI GDGCHC 1 cut(s) 194
SetI ASST 3 cut(s) 59, 152, 159
SfaNI GCATC 1 cut(s) 391
SinI GGWCC 1 cut(s) 302
SmiMI CAYNNNNRTG 1 cut(s) 399
SmlI CTYRAG 1 cut(s) 63
SmoI CTYRAG 1 cut(s) 63
Sse9I AATT 1 cut(s) 499
SsiI CCGC 2 cut(s) 321, 485
TaaI ACNGT 2 cut(s) 258, 272
TasI AATT 1 cut(s) 499
TatI WGTACW 2 cut(s) 180, 258
TauI GCSGC 1 cut(s) 324
Tru1I TTAA 3 cut(s) 263, 467, 522
Tru9I TTAA 3 cut(s) 263, 467, 522
TscAI CASTG 4 cut(s) 137, 414, 495, 516
TseFI GTSAC 2 cut(s) 272, 507
TseI GCWGC 1 cut(s) 514
Tsp45I GTSAC 2 cut(s) 272, 507
TspDTI ATGAA 2 cut(s) 31, 304
TspGWI ACGGA 1 cut(s) 268
TspRI CASTG 4 cut(s) 137, 414, 495, 516
Tth111I GACNNNGTC 1 cut(s) 278
VpaK11BI GGWCC 1 cut(s) 302
XapI RAATTY 1 cut(s) 499
ZrmI AGTACT 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.