pycom03g17720

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0004635
Physical Location & Seq
Reverse (-)
18831553 .. 18833061
1509 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g17720.1

Sequence Viewer

Length: 1101 bp
ATGGAGTTTGGGAGATTTGTTTGGATTCAGATCTTGTTCTTTTCGCTGTGTTTGTGTTTGTGTTCAACTACACAAGGATACAAATTCTATGTTGGTGGGAGAGATGGGTGGGTGTTGAACCCTTCTGAGAACTACAACCACTGGGCTGAGAGGAACAGGTTCAATGTCAATGACAATCTATATTTCAAGTACCAGAAAGGGTCAGACTCAGTGCTCGTCGTGAACAAAGACGACTACTTCAACTGCAACACCCAAAACCCTATTCAGAAACTCGACGGTGGCGACTCAGACTTCACCTTTGACAGGTCTGGAGCTTTCTATTTCATCAGTGGTCAAAATGGTAATTGTGCAAAGGGTCAGAAGCTTCTTGTTACTGTCCTCGCCCCGAGGCACCCCAAGCGACCAGTCCTCCCTCCTCCTCCTACTAGCCCCACCCCTACAGCTCCTGGCCCTAGCCCTACCACCCCTACAGCTCCTGCCTATCCTCCCGCTACGTCTCCTCAGCAGCCCTACCAGCCTCCTGCGGCATCCCCACCACAAGGGCCAGGGCTACCACCAGCTGCAGGCCCAGTCTCTCCATCACCCCCACCTGTGATTACAAACCCTCCTCCATCTGCATCACCAGGCCCATGGTCATCACAACCGTCACCGTCGCCATCAACCTCCCCGGTACCAGCTGCCGGCCCAGTCTCCCCATCACCATCACCTGCGATTACAAACCCTCCTCCAGCTGCCTCACCAGGCCCATGGTCATCACAACCGTCACCATCGCCGTCAACAAATCCGGTGTCAGCTCCCTCAATGACAACATCTCCGGTGCCAGCTCCGTCGTCAGGCTATACTCCAAGCACTTCTCCGCCCTCACCGCCCCCGGAGGGGCTCGCACCTACCCCTTCTGGCGTGCCTGAGGGGCCCACACCTACTCCGTCAAACGAGCCTGCTGGCCCCACACCTCCGGGAACTGTTACTTCTCCGCCGCCAGGGTCCGAGGAGTCGACTGCGCCGAAGCCGTCAAATAATGGAGCGTTACGTGGTGTAACGGCTCCTTCGAGTGTGTTGGTGTCTTTGGCTGTTCTTCTGGTCAGTGTGGCTCTCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

367

Amino Acids

37.59

Weight (kDa)

6.71

Isoelectric Point (pI)

82.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 37 - 120 2.5e-21 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 715
Acc36I ACCTGC 1 cut(s) 715
Acc65I GGTACC 1 cut(s) 670
AccB1I GGYRCC 3 cut(s) 390, 670, 817
AccI GTMKAC 1 cut(s) 995
AciI CCGC 6 cut(s) 489, 524, 857, 866, 974, 977
AcsI RAATTY 1 cut(s) 83
AfaI GTAC 2 cut(s) 191, 672
AfiI CCNNNNNNNGG 8 cut(s) 303, 539, 563, 680, 833, 875, 876, 980
AgsI TTSAA 5 cut(s) 66, 118, 163, 187, 241
AjnI CCWGG 5 cut(s) 445, 544, 622, 739, 979
AjuI GAANNNNNNNTTGG 2 cut(s) 246, 278
AluBI AGCT 9 cut(s) 314, 364, 443, 473, 560, 677, 731, 794, 824
AluI AGCT 9 cut(s) 314, 364, 443, 473, 560, 677, 731, 794, 824
Alw21I GWGCWC 1 cut(s) 216
Alw26I GTCTC 3 cut(s) 501, 577, 694
AlwNI CAGNNNCTG 2 cut(s) 446, 476
Ama87I CYCGRG 1 cut(s) 385
AoxI GGCC 8 cut(s) 448, 542, 565, 625, 682, 742, 911, 943
ApaI GGGCCC 1 cut(s) 915
ApeKI GCWGC 4 cut(s) 505, 560, 677, 731
ApoI RAATTY 1 cut(s) 83
ArsI GACNNNNNNTTYG 4 cut(s) 281, 313, 773, 805
Asp700I GAANNNNTTC 1 cut(s) 158
Asp718I GGTACC 1 cut(s) 670
AspLEI GCGC 1 cut(s) 1003
AsuC2I CCSGG 3 cut(s) 668, 872, 957
AsuHPI GGTGA 9 cut(s) 286, 573, 612, 639, 690, 696, 729, 756, 855
AvaI CYCGRG 1 cut(s) 385
AvaII GGWCC 1 cut(s) 984
AxyI CCTNAGG 1 cut(s) 906
BaeGI GKGCMC 1 cut(s) 915
BanI GGYRCC 3 cut(s) 390, 670, 817
BanII GRGCYC 2 cut(s) 882, 915
Bbv12I GWGCWC 1 cut(s) 216
BbvCI CCTCAGC 1 cut(s) 501
BbvI GCAGC 4 cut(s) 517, 547, 664, 718
BccI CCATC 7 cut(s) 98, 586, 619, 664, 703, 709, 775
BceAI ACGGC 3 cut(s) 757, 994, 1056
BciT130I CCWGG 5 cut(s) 447, 546, 624, 741, 981
BciVI GTATCC 1 cut(s) 71
BcnI CCSGG 3 cut(s) 668, 872, 957
BcoDI GTCTC 3 cut(s) 501, 577, 694
BfaI CTAG 2 cut(s) 426, 453
BfmI CTRYAG 3 cut(s) 438, 468, 561
BfuAI ACCTGC 1 cut(s) 715
BfuI GTATCC 1 cut(s) 71
BglI GCCNNNNNGGC 1 cut(s) 910
BglII AGATCT 1 cut(s) 30
BisI GCNGC 6 cut(s) 506, 525, 561, 678, 732, 977
BlsI GCNGC 6 cut(s) 507, 526, 562, 679, 733, 978
Bme1390I CCNGG 8 cut(s) 447, 546, 624, 668, 741, 872, 957, 981
Bme18I GGWCC 1 cut(s) 984
BmeT110I CYCGRG 1 cut(s) 385
BmiI GGNNCC 8 cut(s) 392, 672, 819, 912, 913, 946, 985, 1044
BmrFI CCNGG 8 cut(s) 447, 546, 624, 668, 741, 872, 957, 981
BmrI ACTGGG 3 cut(s) 151, 563, 680
BmsI GCATC 2 cut(s) 536, 626
BmuI ACTGGG 3 cut(s) 151, 563, 680
BpmI CTGGAG 2 cut(s) 330, 711
Bpu10I CCTNAGC 1 cut(s) 501
BpuMI CCSGG 3 cut(s) 668, 872, 957
BsaAI YACGTR 1 cut(s) 1031
BsaBI GATNNNNATC 1 cut(s) 29
BsaJI CCNNGG 8 cut(s) 386, 545, 629, 666, 746, 870, 980, 987
BsaWI WCCGGW 2 cut(s) 784, 814
Bsc4I CCNNNNNNNGG 8 cut(s) 303, 539, 563, 680, 833, 875, 876, 980
Bse118I RCCGGY 1 cut(s) 680
Bse1I ACTGG 4 cut(s) 146, 404, 569, 686
Bse21I CCTNAGG 1 cut(s) 906
Bse8I GATNNNNATC 1 cut(s) 29
BseBI CCWGG 5 cut(s) 447, 546, 624, 741, 981
BseDI CCNNGG 8 cut(s) 386, 545, 629, 666, 746, 870, 980, 987
BseGI GGATG 1 cut(s) 527
BseJI GATNNNNATC 1 cut(s) 29
BseLI CCNNNNNNNGG 8 cut(s) 303, 539, 563, 680, 833, 875, 876, 980
BseMII CTCAG 6 cut(s) 117, 138, 222, 300, 515, 897
BseNI ACTGG 4 cut(s) 146, 404, 569, 686
BseRI GAGGAG 6 cut(s) 405, 408, 489, 597, 714, 1004
BseSI GKGCMC 1 cut(s) 915
BseXI GCAGC 4 cut(s) 517, 547, 664, 718
BshFI GGCC 8 cut(s) 450, 544, 567, 627, 684, 744, 913, 945
BshNI GGYRCC 3 cut(s) 390, 670, 817
BsiHKAI GWGCWC 1 cut(s) 216
BsiHKCI CYCGRG 1 cut(s) 385
BsiSI CCGG 6 cut(s) 668, 681, 785, 815, 872, 956
BslI CCNNNNNNNGG 8 cut(s) 303, 539, 563, 680, 833, 875, 876, 980
BsmAI GTCTC 3 cut(s) 501, 577, 694
BsmBI CGTCTC 1 cut(s) 501
BsnI GGCC 8 cut(s) 450, 544, 567, 627, 684, 744, 913, 945
BsoBI CYCGRG 1 cut(s) 385
Bsp120I GGGCCC 1 cut(s) 911
Bsp1286I GDGCHC 3 cut(s) 216, 882, 915
Bsp143I GATC 1 cut(s) 30
Bsp19I CCATGG 2 cut(s) 629, 746
BspACI CCGC 6 cut(s) 489, 524, 857, 866, 974, 977
BspANI GGCC 8 cut(s) 450, 544, 567, 627, 684, 744, 913, 945
BspCNI CTCAG 6 cut(s) 118, 139, 221, 299, 514, 898
BspHI TCATGA 1 cut(s) 1097
BspLI GGNNCC 8 cut(s) 392, 672, 819, 912, 913, 946, 985, 1044
BspMAI CTGCAG 1 cut(s) 565
BspMI ACCTGC 1 cut(s) 715
BspT107I GGYRCC 3 cut(s) 390, 670, 817
BsrFI RCCGGY 1 cut(s) 680
BsrI ACTGG 4 cut(s) 146, 404, 569, 686
BssAI RCCGGY 1 cut(s) 680
BssECI CCNNGG 8 cut(s) 386, 545, 629, 666, 746, 870, 980, 987
BssMI GATC 1 cut(s) 30
BssT1I CCWWGG 2 cut(s) 629, 746
Bst2UI CCWGG 5 cut(s) 447, 546, 624, 741, 981
Bst4CI ACNGT 6 cut(s) 278, 376, 645, 651, 762, 964
BstBAI YACGTR 1 cut(s) 1031
BstC8I GCNNGC 7 cut(s) 565, 682, 822, 882, 902, 939, 943
BstDEI CTNAG 6 cut(s) 126, 147, 208, 286, 501, 906
BstDSI CCRYGG 2 cut(s) 629, 746
BstENI CCTNNNNNAGG 1 cut(s) 301
BstF5I GGATG 1 cut(s) 527
BstHHI GCGC 1 cut(s) 1003
BstKTI GATC 1 cut(s) 33
BstMAI GTCTC 3 cut(s) 501, 577, 694
BstMBI GATC 1 cut(s) 30
BstMWI GCNNNNNNNGC 4 cut(s) 397, 514, 865, 910
BstNI CCWGG 5 cut(s) 447, 546, 624, 741, 981
BstSCI CCNGG 8 cut(s) 445, 544, 622, 666, 739, 870, 955, 979
BstSFI CTRYAG 3 cut(s) 438, 468, 561
BstSLI GKGCMC 1 cut(s) 915
BstV1I GCAGC 4 cut(s) 517, 547, 664, 718
BstX2I RGATCY 1 cut(s) 30
BstXI CCANNNNNNTGG 2 cut(s) 630, 747
BstYI RGATCY 1 cut(s) 30
Bsu36I CCTNAGG 1 cut(s) 906
BsuI GTATCC 1 cut(s) 71
BsuRI GGCC 8 cut(s) 450, 544, 567, 627, 684, 744, 913, 945
BtgI CCRYGG 2 cut(s) 629, 746
BtgZI GCGATG 1 cut(s) 753
BtsCI GGATG 1 cut(s) 527
BtsIMutI CAGTG 4 cut(s) 139, 216, 334, 1090
BveI ACCTGC 1 cut(s) 715
Cac8I GCNNGC 7 cut(s) 565, 682, 822, 882, 902, 939, 943
CaiI CAGNNNCTG 2 cut(s) 446, 476
CciI TCATGA 1 cut(s) 1097
CfoI GCGC 1 cut(s) 1003
Cfr10I RCCGGY 1 cut(s) 680
Csp6I GTAC 2 cut(s) 190, 671
CviAII CATG 3 cut(s) 630, 747, 1098
CviQI GTAC 2 cut(s) 190, 671
DdeI CTNAG 6 cut(s) 126, 147, 208, 286, 501, 906
DpnI GATC 1 cut(s) 32
DpnII GATC 1 cut(s) 30
EciI GGCGGA 2 cut(s) 846, 963
Eco130I CCWWGG 2 cut(s) 629, 746
Eco24I GRGCYC 2 cut(s) 882, 915
Eco47I GGWCC 1 cut(s) 984
Eco81I CCTNAGG 1 cut(s) 906
Eco88I CYCGRG 1 cut(s) 385
EcoNI CCTNNNNNAGG 1 cut(s) 301
EcoO109I RGGNCCY 1 cut(s) 911
EcoRII CCWGG 5 cut(s) 445, 544, 622, 739, 979
EcoT14I CCWWGG 2 cut(s) 629, 746
EcoT38I GRGCYC 2 cut(s) 882, 915
ErhI CCWWGG 2 cut(s) 629, 746
Esp3I CGTCTC 1 cut(s) 501
FaeI CATG 3 cut(s) 633, 750, 1101
FaiI YATR 6 cut(s) 90, 181, 631, 748, 840, 1099
FatI CATG 3 cut(s) 629, 746, 1097
FauI CCCGC 1 cut(s) 496
FblI GTMKAC 1 cut(s) 995
Fnu4HI GCNGC 6 cut(s) 506, 525, 561, 678, 732, 977
FokI GGATG 1 cut(s) 514
FriOI GRGCYC 2 cut(s) 882, 915
Fsp4HI GCNGC 6 cut(s) 506, 525, 561, 678, 732, 977
FspBI CTAG 2 cut(s) 426, 453
GlaI GCGC 1 cut(s) 1002
GluI GCNGC 6 cut(s) 506, 525, 561, 678, 732, 977
GsuI CTGGAG 2 cut(s) 330, 711
HaeIII GGCC 8 cut(s) 450, 544, 567, 627, 684, 744, 913, 945
HapII CCGG 6 cut(s) 668, 681, 785, 815, 872, 956
HhaI GCGC 1 cut(s) 1003
Hin1II CATG 3 cut(s) 633, 750, 1101
Hin6I GCGC 1 cut(s) 1001
HinP1I GCGC 1 cut(s) 1001
HincII GTYRAC 2 cut(s) 777, 996
HindII GTYRAC 2 cut(s) 777, 996
HindIII AAGCTT 1 cut(s) 362
HinfI GANTC 4 cut(s) 25, 206, 284, 992
HpaII CCGG 6 cut(s) 668, 681, 785, 815, 872, 956
HphI GGTGA 9 cut(s) 286, 573, 612, 639, 690, 696, 729, 756, 855
Hpy166II GTNNAC 3 cut(s) 223, 777, 996
Hpy188I TCNGA 7 cut(s) 30, 127, 205, 267, 289, 360, 988
Hpy188III TCNNGA 3 cut(s) 220, 309, 1098
Hpy8I GTNNAC 3 cut(s) 223, 777, 996
Hpy99I CGWCG 4 cut(s) 221, 278, 655, 832
HpyAV CCTTC 3 cut(s) 132, 903, 1056
HpyCH4III ACNGT 6 cut(s) 278, 376, 645, 651, 762, 964
HpyCH4IV ACGT 2 cut(s) 494, 1030
HpyCH4V TGCA 4 cut(s) 246, 350, 563, 617
HpyF10VI GCNNNNNNNGC 4 cut(s) 397, 514, 865, 910
HpyF3I CTNAG 6 cut(s) 126, 147, 208, 286, 501, 906
HpySE526I ACGT 2 cut(s) 494, 1030
Hsp92II CATG 3 cut(s) 633, 750, 1101
HspAI GCGC 1 cut(s) 1001
KpnI GGTACC 1 cut(s) 674
KroI GCCGGC 1 cut(s) 680
KroNI GCCGGC 1 cut(s) 682
Kzo9I GATC 1 cut(s) 30
LmnI GCTCC 7 cut(s) 311, 448, 478, 799, 829, 1022, 1048
Lsp1109I GCAGC 4 cut(s) 517, 547, 664, 718
LweI GCATC 2 cut(s) 536, 626
MaeI CTAG 2 cut(s) 426, 453
MaeII ACGT 2 cut(s) 494, 1030
MaeIII GTNAC 6 cut(s) 370, 645, 762, 964, 1026, 1036
MalI GATC 1 cut(s) 32
MboI GATC 1 cut(s) 30
MboII GAAGA 1 cut(s) 1067
MflI RGATCY 1 cut(s) 30
MhlI GDGCHC 3 cut(s) 216, 882, 915
MluCI AATT 2 cut(s) 83, 343
MlyI GAGTC 3 cut(s) 200, 278, 1001
MroNI GCCGGC 1 cut(s) 680
MroXI GAANNNNTTC 1 cut(s) 158
MspA1I CMGCKG 3 cut(s) 560, 677, 731
MspI CCGG 6 cut(s) 668, 681, 785, 815, 872, 956
MspR9I CCNGG 8 cut(s) 447, 546, 624, 668, 741, 872, 957, 981
MvaI CCWGG 5 cut(s) 447, 546, 624, 741, 981
MwoI GCNNNNNNNGC 4 cut(s) 397, 514, 865, 910
NaeI GCCGGC 1 cut(s) 682
NciI CCSGG 3 cut(s) 668, 872, 957
NcoI CCATGG 2 cut(s) 629, 746
NdeII GATC 1 cut(s) 30
NgoMIV GCCGGC 1 cut(s) 680
NlaIII CATG 3 cut(s) 633, 750, 1101
NlaIV GGNNCC 8 cut(s) 392, 672, 819, 912, 913, 946, 985, 1044
NmuCI GTSAC 2 cut(s) 645, 762
PagI TCATGA 1 cut(s) 1097
PaqCI CACCTGC 1 cut(s) 715
PcsI WCGNNNNNNNCGW 3 cut(s) 279, 1001, 1046
PdiI GCCGGC 1 cut(s) 682
PdmI GAANNNNTTC 1 cut(s) 158
PfeI GAWTC 1 cut(s) 25
PfoI TCCNGGA 1 cut(s) 955
PkrI GCNGC 6 cut(s) 507, 526, 562, 679, 733, 978
PleI GAGTC 3 cut(s) 200, 278, 1000
PpsI GAGTC 3 cut(s) 200, 278, 1000
Ppu21I YACGTR 1 cut(s) 1031
Psp6I CCWGG 5 cut(s) 445, 544, 622, 739, 979
PspGI CCWGG 5 cut(s) 445, 544, 622, 739, 979
PspN4I GGNNCC 8 cut(s) 392, 672, 819, 912, 913, 946, 985, 1044
PspOMI GGGCCC 1 cut(s) 911
PstI CTGCAG 1 cut(s) 565
PstNI CAGNNNCTG 2 cut(s) 446, 476
PsuI RGATCY 1 cut(s) 30
PvuII CAGCTG 3 cut(s) 560, 677, 731
RsaI GTAC 2 cut(s) 191, 672
RsaNI GTAC 2 cut(s) 190, 671
SalI GTCGAC 1 cut(s) 994
SatI GCNGC 6 cut(s) 506, 525, 561, 678, 732, 977
Sau3AI GATC 1 cut(s) 30
SchI GAGTC 3 cut(s) 200, 278, 1001
ScrFI CCNGG 8 cut(s) 447, 546, 624, 668, 741, 872, 957, 981
SduI GDGCHC 3 cut(s) 216, 882, 915
SfaNI GCATC 2 cut(s) 536, 626
SfcI CTRYAG 3 cut(s) 438, 468, 561
SinI GGWCC 1 cut(s) 984
Sse9I AATT 2 cut(s) 83, 343
SsiI CCGC 6 cut(s) 489, 524, 857, 866, 974, 977
SspMI CTAG 2 cut(s) 426, 453
StyD4I CCNGG 8 cut(s) 445, 544, 622, 666, 739, 870, 955, 979
StyI CCWWGG 2 cut(s) 629, 746
TaaI ACNGT 6 cut(s) 278, 376, 645, 651, 762, 964
TaiI ACGT 2 cut(s) 497, 1033
TaqI TCGA 3 cut(s) 273, 995, 1049
TasI AATT 2 cut(s) 83, 343
TauI GCSGC 2 cut(s) 527, 979
TfiI GAWTC 1 cut(s) 25
TscAI CASTG 4 cut(s) 146, 216, 334, 1090
TseFI GTSAC 2 cut(s) 645, 762
TseI GCWGC 4 cut(s) 505, 560, 677, 731
Tsp45I GTSAC 2 cut(s) 645, 762
TspDTI ATGAA 1 cut(s) 313
TspGWI ACGGA 2 cut(s) 816, 915
TspRI CASTG 4 cut(s) 146, 216, 334, 1090
VpaK11BI GGWCC 1 cut(s) 984
XagI CCTNNNNNAGG 1 cut(s) 301
XapI RAATTY 1 cut(s) 83
XmiI GTMKAC 1 cut(s) 995
XmnI GAANNNNTTC 1 cut(s) 158
XspI CTAG 2 cut(s) 426, 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.