pycom09g16300

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0004635
Physical Location & Seq
Reverse (-)
16526402 .. 16527154
753 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g16300.1

Sequence Viewer

Length: 630 bp
ATGGGGTCTGAGAGACTATTTTTGAGCATTTTTCTTGTTATTTTGGTGGCGGCATTGGCAAGTAACTCTTCTCAAGCTTACAAGTTCCCTGTGGGTGGCAAAGATGGTTGGGTTTTAAACCCCCCCGAGAGCTTCAATCACTGGGCGGAAAGGCATAGGTTTCAGCTTGTTTTCTTTTGTCGTTGTGGTGAATGCATATTTGCAGTTTTCAACTACAAAAAAGGGTCAAATTCTGTGTTGGTGGTAGCAAAAGAGGATTACAACTCATGCAACACAAAAAACCCAAAACAGTGTTTAACAGATGGGGACTCAGTCTTCAAGTTCAACCGATCGGGGCCATTTTTCTTCATCAGCGGCAATGCTGAAAATTGCCAAAAGGGTCAGAAGCTCATAGTAGTGGTTTTGGCCGTCAGAAACAAAACTCAACACCATACGGATTCTCCTTCTCCATCACCAGTAATTACTGATCCGCCTGCTGTCCTGCCGTCTCCGGCGGATTTGGAACCAAATGCACCAGTACAGCCTCCTCAGCAGAATTCTGCACCAGGATCAGACGTTACGTTAATGGTTTTCATATGTATAATTAGTATTGTTGTGAGTACTCTCATTCTGGGTAACTTTGTGGCTTGA

Protein Analysis

210

Amino Acids

22.82

Weight (kDa)

8.16

Isoelectric Point (pI)

47.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 37 - 128 6.9e-14 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 50, 146, 354, 470, 494
AclWI GGATC 2 cut(s) 461, 556
AcoI YGGCCR 1 cut(s) 405
AcsI RAATTY 2 cut(s) 229, 535
AfaI GTAC 2 cut(s) 519, 601
AfiI CCNNNNNNNGG 1 cut(s) 95
AgsI TTSAA 4 cut(s) 136, 211, 319, 325
AjnI CCWGG 1 cut(s) 544
AluBI AGCT 4 cut(s) 77, 132, 166, 388
AluI AGCT 4 cut(s) 77, 132, 166, 388
Alw26I GTCTC 2 cut(s) 7, 492
AlwI GGATC 2 cut(s) 461, 556
Ama87I CYCGRG 1 cut(s) 125
AoxI GGCC 2 cut(s) 335, 405
ApoI RAATTY 2 cut(s) 229, 535
AspS9I GGNCC 1 cut(s) 335
AsuHPI GGTGA 2 cut(s) 200, 444
AvaI CYCGRG 1 cut(s) 125
BbsI GAAGAC 1 cut(s) 307
BbvCI CCTCAGC 1 cut(s) 528
BccI CCATC 3 cut(s) 98, 296, 457
BceAI ACGGC 2 cut(s) 392, 469
BciT130I CCWGG 1 cut(s) 546
BcoDI GTCTC 2 cut(s) 7, 492
BisI GCNGC 2 cut(s) 51, 355
BlsI GCNGC 2 cut(s) 52, 356
BmcAI AGTACT 1 cut(s) 601
Bme1390I CCNGG 1 cut(s) 546
BmeT110I CYCGRG 1 cut(s) 125
BmgT120I GGNCC 1 cut(s) 335
BmiI GGNNCC 2 cut(s) 336, 504
BmrFI CCNGG 1 cut(s) 546
BmrI ACTGGG 1 cut(s) 151
BmuI ACTGGG 1 cut(s) 151
BpiI GAAGAC 1 cut(s) 307
Bpu10I CCTNAGC 1 cut(s) 528
BpuEI CTTGAG 1 cut(s) 57
BsaXI ACNNNNNCTCC 2 cut(s) 424, 454
Bsc4I CCNNNNNNNGG 1 cut(s) 95
Bse1I ACTGG 3 cut(s) 146, 455, 515
Bse3DI GCAATG 1 cut(s) 364
BseBI CCWGG 1 cut(s) 546
BseLI CCNNNNNNNGG 1 cut(s) 95
BseMI GCAATG 1 cut(s) 364
BseMII CTCAG 2 cut(s) 324, 542
BseNI ACTGG 3 cut(s) 146, 455, 515
BseRI GAGGAG 1 cut(s) 516
BsgI GTGCAG 1 cut(s) 525
Bsh1285I CGRYCG 1 cut(s) 332
BshFI GGCC 2 cut(s) 337, 407
BsiEI CGRYCG 1 cut(s) 332
BsiHKCI CYCGRG 1 cut(s) 125
BsiSI CCGG 1 cut(s) 491
BslFI GGGAC 1 cut(s) 320
BslI CCNNNNNNNGG 1 cut(s) 95
BsmAI GTCTC 2 cut(s) 7, 492
BsmBI CGTCTC 1 cut(s) 492
BsmFI GGGAC 1 cut(s) 320
BsmI GAATGC 1 cut(s) 197
BsnI GGCC 2 cut(s) 337, 407
BsoBI CYCGRG 1 cut(s) 125
Bsp143I GATC 3 cut(s) 329, 466, 548
BspACI CCGC 5 cut(s) 50, 146, 354, 470, 494
BspANI GGCC 2 cut(s) 337, 407
BspCNI CTCAG 2 cut(s) 323, 541
BspLI GGNNCC 2 cut(s) 336, 504
BspPI GGATC 2 cut(s) 461, 556
BsrDI GCAATG 1 cut(s) 364
BsrI ACTGG 3 cut(s) 146, 455, 515
BssMI GATC 3 cut(s) 329, 466, 548
Bst2UI CCWGG 1 cut(s) 546
Bst4CI ACNGT 1 cut(s) 291
Bst6I CTCTTC 1 cut(s) 73
BstC8I GCNNGC 1 cut(s) 474
BstDEI CTNAG 3 cut(s) 9, 310, 528
BstKTI GATC 3 cut(s) 332, 469, 551
BstMAI GTCTC 2 cut(s) 7, 492
BstMBI GATC 3 cut(s) 329, 466, 548
BstMCI CGRYCG 1 cut(s) 332
BstMWI GCNNNNNNNGC 2 cut(s) 56, 529
BstNI CCWGG 1 cut(s) 546
BstSCI CCNGG 1 cut(s) 544
BstV2I GAAGAC 1 cut(s) 307
BsuRI GGCC 2 cut(s) 337, 407
BtsIMutI CAGTG 2 cut(s) 139, 296
Cac8I GCNNGC 1 cut(s) 474
Cfr13I GGNCC 1 cut(s) 335
Csp6I GTAC 2 cut(s) 518, 600
CviAII CATG 1 cut(s) 267
CviJI RGCY 8 cut(s) 77, 132, 166, 337, 388, 407, 523, 626
CviKI_1 RGCY 8 cut(s) 77, 132, 166, 337, 388, 407, 523, 626
CviQI GTAC 2 cut(s) 518, 600
DdeI CTNAG 3 cut(s) 9, 310, 528
DpnI GATC 3 cut(s) 331, 468, 550
DpnII GATC 3 cut(s) 329, 466, 548
DraI TTTAAA 1 cut(s) 117
EaeI YGGCCR 1 cut(s) 405
Eam1104I CTCTTC 1 cut(s) 73
EarI CTCTTC 1 cut(s) 73
EciI GGCGGA 3 cut(s) 161, 459, 509
Eco88I CYCGRG 1 cut(s) 125
EcoRI GAATTC 1 cut(s) 535
EcoRII CCWGG 1 cut(s) 544
EcoT22I ATGCAT 1 cut(s) 197
Esp3I CGTCTC 1 cut(s) 492
FaeI CATG 1 cut(s) 270
FaiI YATR 8 cut(s) 156, 197, 268, 392, 432, 575, 577, 581
FalI AAGNNNNNCTT 2 cut(s) 52, 84
FaqI GGGAC 1 cut(s) 320
FatI CATG 1 cut(s) 266
FauNDI CATATG 1 cut(s) 575
Fnu4HI GCNGC 2 cut(s) 51, 355
Fsp4HI GCNGC 2 cut(s) 51, 355
GluI GCNGC 2 cut(s) 51, 355
HaeIII GGCC 2 cut(s) 337, 407
HapII CCGG 1 cut(s) 491
Hin1II CATG 1 cut(s) 270
HindIII AAGCTT 1 cut(s) 75
HinfI GANTC 2 cut(s) 308, 437
HpaII CCGG 1 cut(s) 491
HphI GGTGA 2 cut(s) 200, 444
Hpy188I TCNGA 4 cut(s) 10, 384, 413, 553
HpyAV CCTTC 1 cut(s) 453
HpyCH4III ACNGT 1 cut(s) 291
HpyCH4IV ACGT 2 cut(s) 555, 560
HpyCH4V TGCA 5 cut(s) 195, 203, 270, 512, 542
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 529
HpyF3I CTNAG 3 cut(s) 9, 310, 528
HpySE526I ACGT 2 cut(s) 555, 560
Hsp92II CATG 1 cut(s) 270
Kzo9I GATC 3 cut(s) 329, 466, 548
MaeII ACGT 2 cut(s) 555, 560
MaeIII GTNAC 3 cut(s) 62, 556, 614
MalI GATC 3 cut(s) 331, 468, 550
MboI GATC 3 cut(s) 329, 466, 548
MboII GAAGA 3 cut(s) 60, 307, 337
MluCI AATT 5 cut(s) 229, 367, 459, 535, 582
MlyI GAGTC 1 cut(s) 302
MnlI CCTC 3 cut(s) 247, 534, 537
Mph1103I ATGCAT 1 cut(s) 197
MseI TTAA 3 cut(s) 116, 296, 563
MslI CAYNNNNRTG 1 cut(s) 395
MspA1I CMGCKG 1 cut(s) 354
MspI CCGG 1 cut(s) 491
MspR9I CCNGG 1 cut(s) 546
Mva1269I GAATGC 1 cut(s) 197
MvaI CCWGG 1 cut(s) 546
MwoI GCNNNNNNNGC 2 cut(s) 56, 529
NdeI CATATG 1 cut(s) 575
NdeII GATC 3 cut(s) 329, 466, 548
NlaIII CATG 1 cut(s) 270
NlaIV GGNNCC 2 cut(s) 336, 504
NsiI ATGCAT 1 cut(s) 197
PctI GAATGC 1 cut(s) 197
PfeI GAWTC 1 cut(s) 437
PflFI GACNNNGTC 1 cut(s) 311
PkrI GCNGC 2 cut(s) 52, 356
Ple19I CGATCG 1 cut(s) 332
PleI GAGTC 1 cut(s) 302
PpsI GAGTC 1 cut(s) 302
Psp6I CCWGG 1 cut(s) 544
PspGI CCWGG 1 cut(s) 544
PspN4I GGNNCC 2 cut(s) 336, 504
PspPI GGNCC 1 cut(s) 335
PsyI GACNNNGTC 1 cut(s) 311
PvuI CGATCG 1 cut(s) 332
RsaI GTAC 2 cut(s) 519, 601
RsaNI GTAC 2 cut(s) 518, 600
RseI CAYNNNNRTG 1 cut(s) 395
SaqAI TTAA 3 cut(s) 116, 296, 563
SatI GCNGC 2 cut(s) 51, 355
Sau3AI GATC 3 cut(s) 329, 466, 548
Sau96I GGNCC 1 cut(s) 335
ScaI AGTACT 1 cut(s) 601
SchI GAGTC 1 cut(s) 302
ScrFI CCNGG 1 cut(s) 546
SetI ASST 7 cut(s) 79, 134, 161, 168, 390, 558, 563
SmiMI CAYNNNNRTG 1 cut(s) 395
SmlI CTYRAG 1 cut(s) 72
SmoI CTYRAG 1 cut(s) 72
Sse9I AATT 5 cut(s) 229, 367, 459, 535, 582
SsiI CCGC 5 cut(s) 50, 146, 354, 470, 494
StyD4I CCNGG 1 cut(s) 544
TaaI ACNGT 1 cut(s) 291
TaiI ACGT 2 cut(s) 558, 563
TasI AATT 5 cut(s) 229, 367, 459, 535, 582
TatI WGTACW 2 cut(s) 517, 599
TauI GCSGC 2 cut(s) 53, 357
TfiI GAWTC 1 cut(s) 437
Tru1I TTAA 3 cut(s) 116, 296, 563
Tru9I TTAA 3 cut(s) 116, 296, 563
TscAI CASTG 2 cut(s) 146, 296
TspDTI ATGAA 2 cut(s) 337, 562
TspGWI ACGGA 1 cut(s) 449
TspRI CASTG 2 cut(s) 146, 296
Tth111I GACNNNGTC 1 cut(s) 311
XapI RAATTY 2 cut(s) 229, 535
ZrmI AGTACT 1 cut(s) 601
Zsp2I ATGCAT 1 cut(s) 197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.