Rh5CG258000

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0004635
Physical Location & Seq
Forward (+)
27399887 .. 27401988
2102 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG258000.1

Sequence Viewer

Length: 975 bp
ATGGAGTTGGGGAGATTTCTATGCCTGTTCTTGGTTTGCTTGACGTGTTTTGTCTCTGCAACAGAAGGATACAAGTTTTACGTTGGTGGGAGAGACGGGTGGGTTTTGAAACCATCTGAGAGTTACAACCACTGGGCTGAGAGGAATAGGTTCCTAGTCAATGACACTCTCTATTTCAAGTACAACAAAGGATCGGACTCGGTTCTGGTGGTGAACAAGGACAGTTTCTTCAACTGCAACACAAGCAACCCAATTCAGAAACTGGATGACGGCAATTCCGAGTTCAAGTTTGATAGGTCTGGACCATTCTATTTCATCAGTGGACAAGATGGTAACTGCGCAAAGGGTCAGAAACTAATTATCTTAGTTCTCGCCGTCAGGCACGGCCAAACTCCTCCGGCTCAGCCTCCCATGGCGGCGCCACCACAAGGCTCAGCGCCAGCACCTGTACCATCAAAAGGCTCAGCCCCAGCTGCTGCACCATCAGGTCCAGCATCAACACCCTCATATGCGCCAAGTACTACCCCAATAACAACTCCAGTTCCAGCTCCATCAAAAGGCTCAGCCCCAGCTGCCGCACCATCAGGTCCAGCATCAACACCCTCATATGCGCCAAGTACTACCCCAATAACAACTCCAGTTCCAGCTCCATCAAAAGGCTCAGCCCCAGCTGCCGGACCATCAGGTCCATCATCATCACCCTCACTTGCGCCAAGTACTACCCCAATAACATCTCCCGTTCCAGCTCCGGCCGCCGCTACTACTCCAACCTCGTCTCCTCCTAGCCCGATGGGGCCCGAACCGACACCGTCCGGAGCTGCTCCTTCCGGGGAAGTGACGTCACCTCCACCGGGGTCGAACCAGGCATCGACAGCACCGAATCAAAACGGAGCGTATGGTGTAACGGCATCGAGCGTGTTGGTGTCTTCGGTTGTTGTTTTGGTCAGTTTGGCTCTTATGAGCAGCTTTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

324

Amino Acids

32.83

Weight (kDa)

8.54

Isoelectric Point (pI)

65.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 34 - 117 5.8e-21 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 684
AatII GACGTC 1 cut(s) 842
Acc16I TGCGCA 1 cut(s) 340
AccB1I GGYRCC 1 cut(s) 418
AccIII TCCGGA 1 cut(s) 812
AciI CCGC 4 cut(s) 416, 576, 753, 756
AclWI GGATC 1 cut(s) 199
AcoI YGGCCR 2 cut(s) 385, 750
AcyI GRCGYC 2 cut(s) 419, 839
AfaI GTAC 5 cut(s) 182, 450, 520, 619, 718
AfiI CCNNNNNNNGG 7 cut(s) 31, 428, 458, 557, 656, 674, 851
AflIII ACRYGT 1 cut(s) 44
AgsI TTSAA 4 cut(s) 109, 178, 232, 286
AjiI CACGTC 1 cut(s) 45
AjnI CCWGG 1 cut(s) 861
AluBI AGCT 8 cut(s) 473, 548, 572, 647, 671, 746, 818, 966
AluI AGCT 8 cut(s) 473, 548, 572, 647, 671, 746, 818, 966
Alw26I GTCTC 3 cut(s) 58, 87, 780
AlwI GGATC 1 cut(s) 199
AlwNI CAGNNNCTG 3 cut(s) 262, 446, 476
Aor13HI TCCGGA 1 cut(s) 812
AoxI GGCC 3 cut(s) 385, 750, 794
ApaI GGGCCC 1 cut(s) 798
ApeKI GCWGC 6 cut(s) 473, 476, 572, 671, 818, 963
Asp700I GAANNNNTTC 1 cut(s) 149
AspLEI GCGC 6 cut(s) 341, 421, 439, 514, 613, 712
AspS9I GGNCC 7 cut(s) 302, 488, 587, 677, 686, 794, 795
AsuC2I CCSGG 2 cut(s) 829, 852
AsuHPI GGTGA 3 cut(s) 223, 690, 834
AvaII GGWCC 5 cut(s) 302, 488, 587, 677, 686
BaeGI GKGCMC 1 cut(s) 798
BanI GGYRCC 1 cut(s) 418
BanII GRGCYC 1 cut(s) 798
BbsI GAAGAC 1 cut(s) 918
BbvI GCAGC 5 cut(s) 460, 463, 559, 658, 805
BceAI ACGGC 4 cut(s) 286, 359, 400, 921
BciT130I CCWGG 1 cut(s) 863
BciVI GTATCC 1 cut(s) 62
BcnI CCSGG 2 cut(s) 829, 852
BcoDI GTCTC 3 cut(s) 58, 87, 780
BfaI CTAG 2 cut(s) 155, 783
BfoI RGCGCY 2 cut(s) 422, 440
BfuI GTATCC 1 cut(s) 62
BlpI GCTNAGC 5 cut(s) 402, 433, 463, 562, 661
BmcAI AGTACT 3 cut(s) 520, 619, 718
Bme1390I CCNGG 3 cut(s) 829, 852, 863
Bme18I GGWCC 5 cut(s) 302, 488, 587, 677, 686
BmgBI CACGTC 1 cut(s) 45
BmgT120I GGNCC 7 cut(s) 302, 488, 587, 677, 686, 794, 795
BmiI GGNNCC 4 cut(s) 152, 420, 795, 796
BmrFI CCNGG 3 cut(s) 829, 852, 863
BmrI ACTGGG 1 cut(s) 142
BmsI GCATC 4 cut(s) 503, 602, 875, 917
BmuI ACTGGG 1 cut(s) 142
BpiI GAAGAC 1 cut(s) 918
BpmI CTGGAG 2 cut(s) 522, 621
Bpu1102I GCTNAGC 5 cut(s) 402, 433, 463, 562, 661
BpuMI CCSGG 2 cut(s) 829, 852
BsaHI GRCGYC 2 cut(s) 419, 839
BsaJI CCNNGG 3 cut(s) 411, 828, 851
BsaWI WCCGGW 1 cut(s) 812
BsaXI ACNNNNNCTCC 4 cut(s) 760, 790, 829, 859
Bsc4I CCNNNNNNNGG 7 cut(s) 31, 428, 458, 557, 656, 674, 851
Bse1I ACTGG 4 cut(s) 137, 267, 539, 638
BseAI TCCGGA 1 cut(s) 812
BseBI CCWGG 1 cut(s) 863
BseDI CCNNGG 3 cut(s) 411, 828, 851
BseGI GGATG 1 cut(s) 271
BseLI CCNNNNNNNGG 7 cut(s) 31, 428, 458, 557, 656, 674, 851
BseMII CTCAG 7 cut(s) 108, 129, 416, 447, 477, 576, 675
BseNI ACTGG 4 cut(s) 137, 267, 539, 638
BseRI GAGGAG 2 cut(s) 384, 768
BseSI GKGCMC 1 cut(s) 798
BseX3I CGGCCG 1 cut(s) 750
BseXI GCAGC 5 cut(s) 460, 463, 559, 658, 805
BseYI CCCAGC 3 cut(s) 469, 568, 667
BsgI GTGCAG 1 cut(s) 462
Bsh1285I CGRYCG 1 cut(s) 753
BshFI GGCC 3 cut(s) 387, 752, 796
BshNI GGYRCC 1 cut(s) 418
BsiEI CGRYCG 1 cut(s) 753
BsiSI CCGG 6 cut(s) 398, 675, 749, 813, 828, 851
BslI CCNNNNNNNGG 7 cut(s) 31, 428, 458, 557, 656, 674, 851
BsmAI GTCTC 3 cut(s) 58, 87, 780
BsmBI CGTCTC 2 cut(s) 87, 780
BsnI GGCC 3 cut(s) 387, 752, 796
Bsp120I GGGCCC 1 cut(s) 794
Bsp1286I GDGCHC 1 cut(s) 798
Bsp13I TCCGGA 1 cut(s) 812
Bsp143I GATC 1 cut(s) 191
Bsp1720I GCTNAGC 5 cut(s) 402, 433, 463, 562, 661
Bsp19I CCATGG 1 cut(s) 411
BspACI CCGC 4 cut(s) 416, 576, 753, 756
BspANI GGCC 3 cut(s) 387, 752, 796
BspCNI CTCAG 7 cut(s) 109, 130, 415, 446, 476, 575, 674
BspEI TCCGGA 1 cut(s) 812
BspLI GGNNCC 4 cut(s) 152, 420, 795, 796
BspPI GGATC 1 cut(s) 199
BspT107I GGYRCC 1 cut(s) 418
BsrI ACTGG 4 cut(s) 137, 267, 539, 638
BssECI CCNNGG 3 cut(s) 411, 828, 851
BssMI GATC 1 cut(s) 191
BssNI GRCGYC 2 cut(s) 419, 839
BssT1I CCWWGG 1 cut(s) 411
Bst2UI CCWGG 1 cut(s) 863
Bst4CI ACNGT 2 cut(s) 224, 810
BstACI GRCGYC 2 cut(s) 419, 839
BstC8I GCNNGC 1 cut(s) 441
BstDEI CTNAG 8 cut(s) 117, 138, 364, 402, 433, 463, 562, 661
BstDSI CCRYGG 1 cut(s) 411
BstF5I GGATG 1 cut(s) 271
BstH2I RGCGCY 2 cut(s) 422, 440
BstHHI GCGC 6 cut(s) 341, 421, 439, 514, 613, 712
BstKTI GATC 1 cut(s) 194
BstMAI GTCTC 3 cut(s) 58, 87, 780
BstMBI GATC 1 cut(s) 191
BstMCI CGRYCG 1 cut(s) 753
BstMWI GCNNNNNNNGC 6 cut(s) 243, 473, 572, 671, 752, 872
BstNI CCWGG 1 cut(s) 863
BstSCI CCNGG 3 cut(s) 827, 850, 861
BstSLI GKGCMC 1 cut(s) 798
BstV1I GCAGC 5 cut(s) 460, 463, 559, 658, 805
BstV2I GAAGAC 1 cut(s) 918
BstZI CGGCCG 1 cut(s) 750
BsuI GTATCC 1 cut(s) 62
BsuRI GGCC 3 cut(s) 387, 752, 796
BtgI CCRYGG 1 cut(s) 411
BtrI CACGTC 1 cut(s) 45
BtsCI GGATG 1 cut(s) 271
BtsIMutI CAGTG 2 cut(s) 130, 325
Cac8I GCNNGC 1 cut(s) 441
CaiI CAGNNNCTG 3 cut(s) 262, 446, 476
CfoI GCGC 6 cut(s) 341, 421, 439, 514, 613, 712
Cfr13I GGNCC 7 cut(s) 302, 488, 587, 677, 686, 794, 795
Csp6I GTAC 5 cut(s) 181, 449, 519, 618, 717
CviAII CATG 1 cut(s) 412
CviQI GTAC 5 cut(s) 181, 449, 519, 618, 717
DdeI CTNAG 8 cut(s) 117, 138, 364, 402, 433, 463, 562, 661
DinI GGCGCC 1 cut(s) 420
DpnI GATC 1 cut(s) 193
DpnII GATC 1 cut(s) 191
DrdI GACNNNNNNGTC 1 cut(s) 684
DseDI GACNNNNNNGTC 1 cut(s) 684
EaeI YGGCCR 2 cut(s) 385, 750
EagI CGGCCG 1 cut(s) 750
EclXI CGGCCG 1 cut(s) 750
Eco130I CCWWGG 1 cut(s) 411
Eco24I GRGCYC 1 cut(s) 798
Eco47I GGWCC 5 cut(s) 302, 488, 587, 677, 686
Eco52I CGGCCG 1 cut(s) 750
EcoO109I RGGNCCY 1 cut(s) 794
EcoRII CCWGG 1 cut(s) 861
EcoT14I CCWWGG 1 cut(s) 411
EcoT38I GRGCYC 1 cut(s) 798
EgeI GGCGCC 1 cut(s) 420
EheI GGCGCC 1 cut(s) 420
ErhI CCWWGG 1 cut(s) 411
Esp3I CGTCTC 2 cut(s) 87, 780
FaeI CATG 1 cut(s) 415
FaiI YATR 8 cut(s) 22, 413, 508, 510, 607, 609, 897, 959
FatI CATG 1 cut(s) 411
FauNDI CATATG 2 cut(s) 508, 607
FokI GGATG 1 cut(s) 278
FriOI GRGCYC 1 cut(s) 798
FspBI CTAG 2 cut(s) 155, 783
FspI TGCGCA 1 cut(s) 340
GlaI GCGC 6 cut(s) 340, 420, 438, 513, 612, 711
GsaI CCCAGC 3 cut(s) 473, 572, 671
GsuI CTGGAG 2 cut(s) 522, 621
HaeII RGCGCY 2 cut(s) 422, 440
HaeIII GGCC 3 cut(s) 387, 752, 796
HapII CCGG 6 cut(s) 398, 675, 749, 813, 828, 851
HhaI GCGC 6 cut(s) 341, 421, 439, 514, 613, 712
Hin1I GRCGYC 2 cut(s) 419, 839
Hin1II CATG 1 cut(s) 415
Hin6I GCGC 6 cut(s) 339, 419, 437, 512, 611, 710
HinP1I GCGC 6 cut(s) 339, 419, 437, 512, 611, 710
HinfI GANTC 2 cut(s) 197, 880
HpaII CCGG 6 cut(s) 398, 675, 749, 813, 828, 851
HphI GGTGA 3 cut(s) 223, 690, 834
Hpy166II GTNNAC 2 cut(s) 214, 323
Hpy188I TCNGA 5 cut(s) 118, 196, 258, 280, 351
Hpy188III TCNNGA 2 cut(s) 300, 813
Hpy8I GTNNAC 2 cut(s) 214, 323
HpyAV CCTTC 2 cut(s) 59, 834
HpyCH4III ACNGT 2 cut(s) 224, 810
HpyCH4IV ACGT 3 cut(s) 44, 81, 839
HpyCH4V TGCA 3 cut(s) 59, 237, 479
HpyF10VI GCNNNNNNNGC 6 cut(s) 243, 473, 572, 671, 752, 872
HpyF3I CTNAG 8 cut(s) 117, 138, 364, 402, 433, 463, 562, 661
HpySE526I ACGT 3 cut(s) 44, 81, 839
Hsp92I GRCGYC 2 cut(s) 419, 839
Hsp92II CATG 1 cut(s) 415
HspAI GCGC 6 cut(s) 339, 419, 437, 512, 611, 710
KasI GGCGCC 1 cut(s) 418
Kpn2I TCCGGA 1 cut(s) 812
Kzo9I GATC 1 cut(s) 191
LmnI GCTCC 6 cut(s) 553, 652, 751, 815, 826, 890
Lsp1109I GCAGC 5 cut(s) 460, 463, 559, 658, 805
LweI GCATC 4 cut(s) 503, 602, 875, 917
MaeI CTAG 2 cut(s) 155, 783
MaeII ACGT 3 cut(s) 44, 81, 839
MaeIII GTNAC 5 cut(s) 122, 332, 835, 840, 901
MalI GATC 1 cut(s) 193
MboI GATC 1 cut(s) 191
MboII GAAGA 2 cut(s) 220, 918
MhlI GDGCHC 1 cut(s) 798
MluCI AATT 3 cut(s) 252, 274, 357
Mly113I GGCGCC 1 cut(s) 419
MlyI GAGTC 1 cut(s) 191
MmeI TCCRAC 1 cut(s) 791
MnlI CCTC 9 cut(s) 135, 405, 417, 514, 613, 712, 781, 789, 855
MroI TCCGGA 1 cut(s) 812
MroXI GAANNNNTTC 1 cut(s) 149
MspA1I CMGCKG 3 cut(s) 473, 572, 671
MspI CCGG 6 cut(s) 398, 675, 749, 813, 828, 851
MspR9I CCNGG 3 cut(s) 829, 852, 863
MvaI CCWGG 1 cut(s) 863
MwoI GCNNNNNNNGC 6 cut(s) 243, 473, 572, 671, 752, 872
NarI GGCGCC 1 cut(s) 419
NciI CCSGG 2 cut(s) 829, 852
NcoI CCATGG 1 cut(s) 411
NdeI CATATG 2 cut(s) 508, 607
NdeII GATC 1 cut(s) 191
NlaIII CATG 1 cut(s) 415
NlaIV GGNNCC 4 cut(s) 152, 420, 795, 796
NmuCI GTSAC 2 cut(s) 835, 840
NsbI TGCGCA 1 cut(s) 340
PcsI WCGNNNNNNNCGW 2 cut(s) 276, 875
PdmI GAANNNNTTC 1 cut(s) 149
PfeI GAWTC 1 cut(s) 880
PflFI GACNNNGTC 1 cut(s) 808
PleI GAGTC 1 cut(s) 191
PluTI GGCGCC 1 cut(s) 422
PpsI GAGTC 1 cut(s) 191
Psp6I CCWGG 1 cut(s) 861
PspFI CCCAGC 3 cut(s) 469, 568, 667
PspGI CCWGG 1 cut(s) 861
PspN4I GGNNCC 4 cut(s) 152, 420, 795, 796
PspOMI GGGCCC 1 cut(s) 794
PspPI GGNCC 7 cut(s) 302, 488, 587, 677, 686, 794, 795
PstNI CAGNNNCTG 3 cut(s) 262, 446, 476
PsyI GACNNNGTC 1 cut(s) 808
PvuII CAGCTG 3 cut(s) 473, 572, 671
RsaI GTAC 5 cut(s) 182, 450, 520, 619, 718
RsaNI GTAC 5 cut(s) 181, 449, 519, 618, 717
Sau3AI GATC 1 cut(s) 191
Sau96I GGNCC 7 cut(s) 302, 488, 587, 677, 686, 794, 795
ScaI AGTACT 3 cut(s) 520, 619, 718
SchI GAGTC 1 cut(s) 191
ScrFI CCNGG 3 cut(s) 829, 852, 863
SduI GDGCHC 1 cut(s) 798
SfaNI GCATC 4 cut(s) 503, 602, 875, 917
SfoI GGCGCC 1 cut(s) 420
SinI GGWCC 5 cut(s) 302, 488, 587, 677, 686
Sse9I AATT 3 cut(s) 252, 274, 357
SsiI CCGC 4 cut(s) 416, 576, 753, 756
SspDI GGCGCC 1 cut(s) 418
SspMI CTAG 2 cut(s) 155, 783
StyD4I CCNGG 3 cut(s) 827, 850, 861
StyI CCWWGG 1 cut(s) 411
TaaI ACNGT 2 cut(s) 224, 810
TaiI ACGT 3 cut(s) 47, 84, 842
TaqI TCGA 3 cut(s) 857, 869, 911
TasI AATT 3 cut(s) 252, 274, 357
TatI WGTACW 4 cut(s) 180, 518, 617, 716
TauI GCSGC 4 cut(s) 419, 578, 755, 758
TfiI GAWTC 1 cut(s) 880
TscAI CASTG 2 cut(s) 137, 325
TseFI GTSAC 2 cut(s) 835, 840
TseI GCWGC 6 cut(s) 473, 476, 572, 671, 818, 963
Tsp45I GTSAC 2 cut(s) 835, 840
TspDTI ATGAA 1 cut(s) 304
TspGWI ACGGA 1 cut(s) 903
TspRI CASTG 2 cut(s) 137, 325
Tth111I GACNNNGTC 1 cut(s) 808
VpaK11BI GGWCC 5 cut(s) 302, 488, 587, 677, 686
XmnI GAANNNNTTC 1 cut(s) 149
XspI CTAG 2 cut(s) 155, 783
ZraI GACGTC 1 cut(s) 840
ZrmI AGTACT 3 cut(s) 520, 619, 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.