Rh5BG230100

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0004635
Physical Location & Seq
Forward (+)
27043258 .. 27044534
1277 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG230100.1

Sequence Viewer

Length: 876 bp
ATGGAGTTGGGGAGATTTCTATGCCTGTTCTTGGTTTGCTTGACGTGTTTTGTCTCTGCAACAGAAGGATACAAGTTTTACGTTGGTGGGAGAGACGGGTGGGTTTTGAAACCATCTGAGAGTTACAACCACTGGGCTGAGAGGAATAGGTTCCTAGTCAATGACACCCTCTATTTCAAGTACAACAAAGGATCGGACTCGGTTCTGGTGGTGAACAAGGACAGTTTCTACAACTGCAACACAAGCAACCCTATTCAGAAACTGGATGACGGCAATTCCGAGTTCAAGTTTGATAGGTCTGGACCATTCTATTTCATCAGTGGACAAGATGGTAACTGCGCAAAGGGTCAGAAACTAATTATCATAGTTCTCGCCGTCAGGCACCGCCAAACTCCTCCGGCTCAGCCTCCCATGGCGGCGCCACCACAAGGCTCAGCGCCAGCACCTGTACCATCAAAAGGCTCAGCCCCAGCTGCTGCACCATCAGGTCCAGCATCAACACCCTCATATGCGCCAAGTACTACCCCAATAACAACTCCAGTTCCAGCTCCATCAAAAGGCTCAGCCCCAGCTGCCGCACCATCAGGTCCATCATCATCACCCTCACTTGCGCCAAGTACTACCCCAATAACATCTCCAGTTCCAGCTCCGGCCGCCGCTACTACTCCAACCTCGTCTCCTCCTAGCCCGGTGGGGCCCGAACCGACACCTTCCGGAGCTGCTCCTTCCGGGGAAGTGACGTCACCTCCACCGGGGTCGAACCAGGCATCGACAGCACCGAATCAAAACGGAGCGTATGGTGTAACGGCATCGAGCGTGTTGGTGTCTTCGGTTGTTGTTTTGGTCAGTTTGGCTCTTATGAGCAGCTTTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

291

Amino Acids

29.91

Weight (kDa)

8.55

Isoelectric Point (pI)

64.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 34 - 117 2.7e-21 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 743
Acc16I TGCGCA 1 cut(s) 340
AccB1I GGYRCC 2 cut(s) 381, 418
AccIII TCCGGA 1 cut(s) 713
AciI CCGC 5 cut(s) 385, 416, 576, 654, 657
AclWI GGATC 1 cut(s) 199
AcoI YGGCCR 1 cut(s) 651
AcyI GRCGYC 2 cut(s) 419, 740
AfaI GTAC 4 cut(s) 182, 450, 520, 619
AfiI CCNNNNNNNGG 5 cut(s) 31, 428, 458, 557, 752
AflIII ACRYGT 1 cut(s) 44
AgsI TTSAA 3 cut(s) 109, 178, 286
AjiI CACGTC 1 cut(s) 45
AjnI CCWGG 1 cut(s) 762
AluBI AGCT 6 cut(s) 473, 548, 572, 647, 719, 867
AluI AGCT 6 cut(s) 473, 548, 572, 647, 719, 867
Alw26I GTCTC 3 cut(s) 58, 87, 681
AlwI GGATC 1 cut(s) 199
AlwNI CAGNNNCTG 3 cut(s) 262, 446, 476
Aor13HI TCCGGA 1 cut(s) 713
AoxI GGCC 2 cut(s) 651, 695
ApaI GGGCCC 1 cut(s) 699
ApeKI GCWGC 5 cut(s) 473, 476, 572, 719, 864
Asp700I GAANNNNTTC 1 cut(s) 149
AspLEI GCGC 5 cut(s) 341, 421, 439, 514, 613
AspS9I GGNCC 5 cut(s) 302, 488, 587, 695, 696
AsuC2I CCSGG 3 cut(s) 689, 730, 753
AsuHPI GGTGA 3 cut(s) 223, 591, 735
AvaII GGWCC 3 cut(s) 302, 488, 587
BaeGI GKGCMC 1 cut(s) 699
BanI GGYRCC 2 cut(s) 381, 418
BanII GRGCYC 1 cut(s) 699
BbsI GAAGAC 1 cut(s) 819
BbvI GCAGC 4 cut(s) 460, 463, 559, 706
BccI CCATC 7 cut(s) 121, 323, 460, 490, 559, 589, 598
BceAI ACGGC 3 cut(s) 286, 359, 822
BciT130I CCWGG 1 cut(s) 764
BciVI GTATCC 1 cut(s) 62
BcnI CCSGG 3 cut(s) 689, 730, 753
BcoDI GTCTC 3 cut(s) 58, 87, 681
BfaI CTAG 2 cut(s) 155, 684
BfoI RGCGCY 2 cut(s) 422, 440
BfuI GTATCC 1 cut(s) 62
BisI GCNGC 9 cut(s) 417, 474, 477, 573, 576, 654, 657, 720, 865
BlpI GCTNAGC 4 cut(s) 402, 433, 463, 562
BlsI GCNGC 9 cut(s) 418, 475, 478, 574, 577, 655, 658, 721, 866
BmcAI AGTACT 2 cut(s) 520, 619
Bme1390I CCNGG 4 cut(s) 689, 730, 753, 764
Bme18I GGWCC 3 cut(s) 302, 488, 587
BmgBI CACGTC 1 cut(s) 45
BmgT120I GGNCC 5 cut(s) 302, 488, 587, 695, 696
BmiI GGNNCC 5 cut(s) 152, 383, 420, 696, 697
BmrFI CCNGG 4 cut(s) 689, 730, 753, 764
BmrI ACTGGG 1 cut(s) 142
BmsI GCATC 3 cut(s) 503, 776, 818
BmuI ACTGGG 1 cut(s) 142
BpiI GAAGAC 1 cut(s) 819
BpmI CTGGAG 2 cut(s) 522, 621
Bpu1102I GCTNAGC 4 cut(s) 402, 433, 463, 562
BpuMI CCSGG 3 cut(s) 689, 730, 753
BsaHI GRCGYC 2 cut(s) 419, 740
BsaJI CCNNGG 3 cut(s) 411, 729, 752
BsaWI WCCGGW 1 cut(s) 713
BsaXI ACNNNNNCTCC 4 cut(s) 661, 691, 730, 760
Bsc4I CCNNNNNNNGG 5 cut(s) 31, 428, 458, 557, 752
Bse1I ACTGG 4 cut(s) 137, 267, 539, 638
BseAI TCCGGA 1 cut(s) 713
BseBI CCWGG 1 cut(s) 764
BseDI CCNNGG 3 cut(s) 411, 729, 752
BseGI GGATG 1 cut(s) 271
BseLI CCNNNNNNNGG 5 cut(s) 31, 428, 458, 557, 752
BseMII CTCAG 6 cut(s) 108, 129, 416, 447, 477, 576
BseNI ACTGG 4 cut(s) 137, 267, 539, 638
BseRI GAGGAG 2 cut(s) 384, 669
BseSI GKGCMC 1 cut(s) 699
BseX3I CGGCCG 1 cut(s) 651
BseXI GCAGC 4 cut(s) 460, 463, 559, 706
BseYI CCCAGC 2 cut(s) 469, 568
BsgI GTGCAG 1 cut(s) 462
Bsh1285I CGRYCG 1 cut(s) 654
BshFI GGCC 2 cut(s) 653, 697
BshNI GGYRCC 2 cut(s) 381, 418
BsiEI CGRYCG 1 cut(s) 654
BsiSI CCGG 6 cut(s) 398, 650, 689, 714, 729, 752
BslI CCNNNNNNNGG 5 cut(s) 31, 428, 458, 557, 752
BsmAI GTCTC 3 cut(s) 58, 87, 681
BsmBI CGTCTC 2 cut(s) 87, 681
BsnI GGCC 2 cut(s) 653, 697
Bsp120I GGGCCC 1 cut(s) 695
Bsp1286I GDGCHC 1 cut(s) 699
Bsp13I TCCGGA 1 cut(s) 713
Bsp143I GATC 1 cut(s) 191
Bsp1720I GCTNAGC 4 cut(s) 402, 433, 463, 562
Bsp19I CCATGG 1 cut(s) 411
BspACI CCGC 5 cut(s) 385, 416, 576, 654, 657
BspANI GGCC 2 cut(s) 653, 697
BspCNI CTCAG 6 cut(s) 109, 130, 415, 446, 476, 575
BspEI TCCGGA 1 cut(s) 713
BspLI GGNNCC 5 cut(s) 152, 383, 420, 696, 697
BspPI GGATC 1 cut(s) 199
BspT107I GGYRCC 2 cut(s) 381, 418
BsrI ACTGG 4 cut(s) 137, 267, 539, 638
BssECI CCNNGG 3 cut(s) 411, 729, 752
BssMI GATC 1 cut(s) 191
BssNI GRCGYC 2 cut(s) 419, 740
BssT1I CCWWGG 1 cut(s) 411
Bst2UI CCWGG 1 cut(s) 764
Bst4CI ACNGT 1 cut(s) 224
BstACI GRCGYC 2 cut(s) 419, 740
BstC8I GCNNGC 1 cut(s) 441
BstDEI CTNAG 6 cut(s) 117, 138, 402, 433, 463, 562
BstDSI CCRYGG 1 cut(s) 411
BstF5I GGATG 1 cut(s) 271
BstH2I RGCGCY 2 cut(s) 422, 440
BstHHI GCGC 5 cut(s) 341, 421, 439, 514, 613
BstKTI GATC 1 cut(s) 194
BstMAI GTCTC 3 cut(s) 58, 87, 681
BstMBI GATC 1 cut(s) 191
BstMCI CGRYCG 1 cut(s) 654
BstMWI GCNNNNNNNGC 5 cut(s) 243, 473, 572, 653, 773
BstNI CCWGG 1 cut(s) 764
BstSCI CCNGG 4 cut(s) 687, 728, 751, 762
BstSLI GKGCMC 1 cut(s) 699
BstV1I GCAGC 4 cut(s) 460, 463, 559, 706
BstV2I GAAGAC 1 cut(s) 819
BstZI CGGCCG 1 cut(s) 651
BsuI GTATCC 1 cut(s) 62
BsuRI GGCC 2 cut(s) 653, 697
BtgI CCRYGG 1 cut(s) 411
BtrI CACGTC 1 cut(s) 45
BtsCI GGATG 1 cut(s) 271
BtsIMutI CAGTG 2 cut(s) 130, 325
Cac8I GCNNGC 1 cut(s) 441
CaiI CAGNNNCTG 3 cut(s) 262, 446, 476
CfoI GCGC 5 cut(s) 341, 421, 439, 514, 613
Cfr13I GGNCC 5 cut(s) 302, 488, 587, 695, 696
Csp6I GTAC 4 cut(s) 181, 449, 519, 618
CviAII CATG 1 cut(s) 412
CviQI GTAC 4 cut(s) 181, 449, 519, 618
DdeI CTNAG 6 cut(s) 117, 138, 402, 433, 463, 562
DinI GGCGCC 1 cut(s) 420
DpnI GATC 1 cut(s) 193
DpnII GATC 1 cut(s) 191
EaeI YGGCCR 1 cut(s) 651
EagI CGGCCG 1 cut(s) 651
EclXI CGGCCG 1 cut(s) 651
Eco130I CCWWGG 1 cut(s) 411
Eco24I GRGCYC 1 cut(s) 699
Eco47I GGWCC 3 cut(s) 302, 488, 587
Eco52I CGGCCG 1 cut(s) 651
EcoO109I RGGNCCY 1 cut(s) 695
EcoRII CCWGG 1 cut(s) 762
EcoT14I CCWWGG 1 cut(s) 411
EcoT38I GRGCYC 1 cut(s) 699
EgeI GGCGCC 1 cut(s) 420
EheI GGCGCC 1 cut(s) 420
ErhI CCWWGG 1 cut(s) 411
Esp3I CGTCTC 2 cut(s) 87, 681
FaeI CATG 1 cut(s) 415
FaiI YATR 7 cut(s) 22, 365, 413, 508, 510, 798, 860
FatI CATG 1 cut(s) 411
FauNDI CATATG 1 cut(s) 508
Fnu4HI GCNGC 9 cut(s) 417, 474, 477, 573, 576, 654, 657, 720, 865
FokI GGATG 1 cut(s) 278
FriOI GRGCYC 1 cut(s) 699
Fsp4HI GCNGC 9 cut(s) 417, 474, 477, 573, 576, 654, 657, 720, 865
FspBI CTAG 2 cut(s) 155, 684
FspI TGCGCA 1 cut(s) 340
GlaI GCGC 5 cut(s) 340, 420, 438, 513, 612
GluI GCNGC 9 cut(s) 417, 474, 477, 573, 576, 654, 657, 720, 865
GsaI CCCAGC 2 cut(s) 473, 572
GsuI CTGGAG 2 cut(s) 522, 621
HaeII RGCGCY 2 cut(s) 422, 440
HaeIII GGCC 2 cut(s) 653, 697
HapII CCGG 6 cut(s) 398, 650, 689, 714, 729, 752
HhaI GCGC 5 cut(s) 341, 421, 439, 514, 613
Hin1I GRCGYC 2 cut(s) 419, 740
Hin1II CATG 1 cut(s) 415
Hin6I GCGC 5 cut(s) 339, 419, 437, 512, 611
HinP1I GCGC 5 cut(s) 339, 419, 437, 512, 611
HinfI GANTC 2 cut(s) 197, 781
HpaII CCGG 6 cut(s) 398, 650, 689, 714, 729, 752
HphI GGTGA 3 cut(s) 223, 591, 735
Hpy166II GTNNAC 2 cut(s) 214, 323
Hpy188I TCNGA 5 cut(s) 118, 196, 258, 280, 351
Hpy188III TCNNGA 2 cut(s) 300, 714
Hpy8I GTNNAC 2 cut(s) 214, 323
HpyAV CCTTC 3 cut(s) 59, 720, 735
HpyCH4III ACNGT 1 cut(s) 224
HpyCH4IV ACGT 3 cut(s) 44, 81, 740
HpyCH4V TGCA 3 cut(s) 59, 237, 479
HpyF10VI GCNNNNNNNGC 5 cut(s) 243, 473, 572, 653, 773
HpyF3I CTNAG 6 cut(s) 117, 138, 402, 433, 463, 562
HpySE526I ACGT 3 cut(s) 44, 81, 740
Hsp92I GRCGYC 2 cut(s) 419, 740
Hsp92II CATG 1 cut(s) 415
HspAI GCGC 5 cut(s) 339, 419, 437, 512, 611
KasI GGCGCC 1 cut(s) 418
Kpn2I TCCGGA 1 cut(s) 713
Kzo9I GATC 1 cut(s) 191
LmnI GCTCC 5 cut(s) 553, 652, 716, 727, 791
Lsp1109I GCAGC 4 cut(s) 460, 463, 559, 706
LweI GCATC 3 cut(s) 503, 776, 818
MaeI CTAG 2 cut(s) 155, 684
MaeII ACGT 3 cut(s) 44, 81, 740
MaeIII GTNAC 5 cut(s) 122, 332, 736, 741, 802
MalI GATC 1 cut(s) 193
MboI GATC 1 cut(s) 191
MboII GAAGA 1 cut(s) 819
MhlI GDGCHC 1 cut(s) 699
MluCI AATT 2 cut(s) 274, 357
Mly113I GGCGCC 1 cut(s) 419
MlyI GAGTC 1 cut(s) 191
MmeI TCCRAC 1 cut(s) 692
MnlI CCTC 9 cut(s) 135, 179, 405, 417, 514, 613, 682, 690, 756
MroI TCCGGA 1 cut(s) 713
MroXI GAANNNNTTC 1 cut(s) 149
MspA1I CMGCKG 2 cut(s) 473, 572
MspI CCGG 6 cut(s) 398, 650, 689, 714, 729, 752
MspR9I CCNGG 4 cut(s) 689, 730, 753, 764
MvaI CCWGG 1 cut(s) 764
MwoI GCNNNNNNNGC 5 cut(s) 243, 473, 572, 653, 773
NarI GGCGCC 1 cut(s) 419
NciI CCSGG 3 cut(s) 689, 730, 753
NcoI CCATGG 1 cut(s) 411
NdeI CATATG 1 cut(s) 508
NdeII GATC 1 cut(s) 191
NlaIII CATG 1 cut(s) 415
NlaIV GGNNCC 5 cut(s) 152, 383, 420, 696, 697
NmuCI GTSAC 2 cut(s) 736, 741
NsbI TGCGCA 1 cut(s) 340
PcsI WCGNNNNNNNCGW 2 cut(s) 276, 776
PdmI GAANNNNTTC 1 cut(s) 149
PfeI GAWTC 1 cut(s) 781
PkrI GCNGC 9 cut(s) 418, 475, 478, 574, 577, 655, 658, 721, 866
PleI GAGTC 1 cut(s) 191
PluTI GGCGCC 1 cut(s) 422
PpsI GAGTC 1 cut(s) 191
Psp6I CCWGG 1 cut(s) 762
PspFI CCCAGC 2 cut(s) 469, 568
PspGI CCWGG 1 cut(s) 762
PspN4I GGNNCC 5 cut(s) 152, 383, 420, 696, 697
PspOMI GGGCCC 1 cut(s) 695
PspPI GGNCC 5 cut(s) 302, 488, 587, 695, 696
PstNI CAGNNNCTG 3 cut(s) 262, 446, 476
PvuII CAGCTG 2 cut(s) 473, 572
RsaI GTAC 4 cut(s) 182, 450, 520, 619
RsaNI GTAC 4 cut(s) 181, 449, 519, 618
SatI GCNGC 9 cut(s) 417, 474, 477, 573, 576, 654, 657, 720, 865
Sau3AI GATC 1 cut(s) 191
Sau96I GGNCC 5 cut(s) 302, 488, 587, 695, 696
ScaI AGTACT 2 cut(s) 520, 619
SchI GAGTC 1 cut(s) 191
ScrFI CCNGG 4 cut(s) 689, 730, 753, 764
SduI GDGCHC 1 cut(s) 699
SfaNI GCATC 3 cut(s) 503, 776, 818
SfoI GGCGCC 1 cut(s) 420
SinI GGWCC 3 cut(s) 302, 488, 587
Sse9I AATT 2 cut(s) 274, 357
SsiI CCGC 5 cut(s) 385, 416, 576, 654, 657
SspDI GGCGCC 1 cut(s) 418
SspMI CTAG 2 cut(s) 155, 684
StyD4I CCNGG 4 cut(s) 687, 728, 751, 762
StyI CCWWGG 1 cut(s) 411
TaaI ACNGT 1 cut(s) 224
TaiI ACGT 3 cut(s) 47, 84, 743
TaqI TCGA 3 cut(s) 758, 770, 812
TasI AATT 2 cut(s) 274, 357
TatI WGTACW 3 cut(s) 180, 518, 617
TauI GCSGC 4 cut(s) 419, 578, 656, 659
TfiI GAWTC 1 cut(s) 781
TscAI CASTG 2 cut(s) 137, 325
TseFI GTSAC 2 cut(s) 736, 741
TseI GCWGC 5 cut(s) 473, 476, 572, 719, 864
Tsp45I GTSAC 2 cut(s) 736, 741
TspDTI ATGAA 1 cut(s) 304
TspGWI ACGGA 1 cut(s) 804
TspRI CASTG 2 cut(s) 137, 325
VpaK11BI GGWCC 3 cut(s) 302, 488, 587
XmnI GAANNNNTTC 1 cut(s) 149
XspI CTAG 2 cut(s) 155, 684
ZraI GACGTC 1 cut(s) 741
ZrmI AGTACT 2 cut(s) 520, 619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.