pycom11g22140

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0004635
Physical Location & Seq
Reverse (-)
25138049 .. 25139642
1594 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g22140.2

Sequence Viewer

Length: 912 bp
ATGGAGTTTGGGAGATTTGTTTGTCTTCAAATCTTGTTGGTGTTCTTTTGCTTGTGTTTGTCATCCATAGAAGGATACAAATTCTATGTTGGTGGGAAAGATGGGTGGGTTGTGAACCCTTCTCAGAGCTACAGCCTCTGGGCTGAGAGAAACAGGTTCAATATCAATGACACACTACATTTCAAGTACAAGAAGGGGTCAGACTCGGTGCTCGTCGTGAACAAAGACGACTACTTCAGCTGCAACACCCAAAACCCTATTCAGAAACTCGACGGTGGCGACTCAGACTTCACCCTCGATAGGTCTGGACCTTTCTACTTTATTAGTGGCCAAAATGGTAATTGTCAAAAGGGTCAGAAGCTTCTTGTTATTGTCTTGGCCCCGAGGCACCCCAAGCCACCAGTCCTCCCTCCTCCTACTACTAGCCCTACCGCTCCTCCGCAGCCCTACCACCCTCCTGCTGCGTCCCCACCACAAGGGCCCGGGCTACCCCCAGCTGCTGGGGGCCCGGTCTCTCTATCTCCATCACCAGTGATTACAACTCCTCCATCTGCTTCACCAGGGCCAGGGTCATCACAACCGTCGCCATCGCCGTCAACCTCTCCGGTTCCAGCTCCGTCAACCAACACTCCAACATCATCTCCGGTGCCAGCTCCGGCGTCAGGCTATACTCCAACTACTTCTCCACCCGCACCGCCTCCGGAGGGGGCCGCACCTACCCCATCTGGCGTGCCTGGGGGCGCTACACCTACTCCATCCGGCCAGCCTGCGGGCCCGCCGGGAACTGTTACTTCTCCGCCACCAGGGTCCCAGGAAGCGACTGCCCCGTCTGGAGCGTTACGTGGAGTAGCGGCTCCTTCGATTGTGTTGGTGTCTTTGGCTGTTGTTCTGGTCAGTGTGGCGCTCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

304

Amino Acids

30.87

Weight (kDa)

8.25

Isoelectric Point (pI)

75.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 826
AccB1I GGYRCC 2 cut(s) 387, 646
AccB7I CCANNNNNTGG 1 cut(s) 500
AccBSI CCGCTC 1 cut(s) 434
AccIII TCCGGA 1 cut(s) 700
AciI CCGC 9 cut(s) 432, 440, 690, 695, 711, 770, 776, 797, 851
AcoI YGGCCR 2 cut(s) 328, 760
AcsI RAATTY 1 cut(s) 80
AcuI CTGAAG 1 cut(s) 220
AcyI GRCGYC 1 cut(s) 659
AfaI GTAC 1 cut(s) 188
AfiI CCNNNNNNNGG 8 cut(s) 300, 476, 500, 566, 662, 704, 769, 803
AgsI TTSAA 3 cut(s) 29, 160, 184
AjnI CCWGG 5 cut(s) 559, 565, 733, 802, 810
AjuI GAANNNNNNNTTGG 2 cut(s) 243, 275
AluBI AGCT 6 cut(s) 129, 240, 361, 497, 614, 653
AluI AGCT 6 cut(s) 129, 240, 361, 497, 614, 653
Alw21I GWGCWC 1 cut(s) 213
Alw26I GTCTC 1 cut(s) 517
AlwNI CAGNNNCTG 2 cut(s) 138, 500
Ama87I CYCGRG 2 cut(s) 382, 482
Aor13HI TCCGGA 1 cut(s) 700
AoxI GGCC 8 cut(s) 328, 378, 479, 505, 563, 708, 760, 772
ApaI GGGCCC 3 cut(s) 483, 509, 776
ApeKI GCWGC 4 cut(s) 240, 442, 461, 497
ApoI RAATTY 1 cut(s) 80
AspLEI GCGC 2 cut(s) 743, 904
AsuC2I CCSGG 4 cut(s) 483, 484, 509, 780
AsuHPI GGTGA 3 cut(s) 283, 519, 549
AvaI CYCGRG 2 cut(s) 382, 482
AvaII GGWCC 2 cut(s) 308, 807
BaeGI GKGCMC 3 cut(s) 483, 509, 776
BalI TGGCCA 1 cut(s) 330
BanI GGYRCC 2 cut(s) 387, 646
BanII GRGCYC 3 cut(s) 483, 509, 776
BbsI GAAGAC 1 cut(s) 17
Bbv12I GWGCWC 1 cut(s) 213
BbvI GCAGC 4 cut(s) 227, 448, 454, 484
BccI CCATC 6 cut(s) 95, 532, 556, 595, 730, 763
BceAI ACGGC 1 cut(s) 577
BciT130I CCWGG 5 cut(s) 561, 567, 735, 804, 812
BciVI GTATCC 1 cut(s) 68
BcnI CCSGG 4 cut(s) 483, 484, 509, 780
BcoDI GTCTC 1 cut(s) 517
BfaI CTAG 1 cut(s) 423
BfmI CTRYAG 1 cut(s) 130
BfoI RGCGCY 2 cut(s) 744, 905
BfuI GTATCC 1 cut(s) 68
BisI GCNGC 6 cut(s) 241, 443, 462, 498, 711, 852
BlsI GCNGC 6 cut(s) 242, 444, 463, 499, 712, 853
Bme1390I CCNGG 9 cut(s) 483, 484, 509, 561, 567, 735, 780, 804, 812
Bme18I GGWCC 2 cut(s) 308, 807
BmeT110I CYCGRG 2 cut(s) 382, 482
BmrFI CCNGG 9 cut(s) 483, 484, 509, 561, 567, 735, 780, 804, 812
BpiI GAAGAC 1 cut(s) 17
BpmI CTGGAG 1 cut(s) 852
BpuMI CCSGG 4 cut(s) 483, 484, 509, 780
BsaAI YACGTR 1 cut(s) 842
BsaHI GRCGYC 1 cut(s) 659
BsaI GGTCTC 1 cut(s) 517
BsaJI CCNNGG 7 cut(s) 383, 482, 560, 566, 734, 803, 810
BsaWI WCCGGW 3 cut(s) 604, 643, 700
Bsc4I CCNNNNNNNGG 8 cut(s) 300, 476, 500, 566, 662, 704, 769, 803
Bse1I ACTGG 2 cut(s) 401, 530
BseAI TCCGGA 1 cut(s) 700
BseBI CCWGG 5 cut(s) 561, 567, 735, 804, 812
BseDI CCNNGG 7 cut(s) 383, 482, 560, 566, 734, 803, 810
BseGI GGATG 2 cut(s) 62, 755
BseLI CCNNNNNNNGG 8 cut(s) 300, 476, 500, 566, 662, 704, 769, 803
BseMII CTCAG 3 cut(s) 135, 137, 297
BseNI ACTGG 2 cut(s) 401, 530
BseRI GAGGAG 3 cut(s) 402, 426, 534
BseSI GKGCMC 3 cut(s) 483, 509, 776
BseXI GCAGC 4 cut(s) 227, 448, 454, 484
BseYI CCCAGC 2 cut(s) 493, 500
BshFI GGCC 8 cut(s) 330, 380, 481, 507, 565, 710, 762, 774
BshNI GGYRCC 2 cut(s) 387, 646
BsiHKAI GWGCWC 1 cut(s) 213
BsiHKCI CYCGRG 2 cut(s) 382, 482
BsiSI CCGG 8 cut(s) 483, 509, 605, 644, 656, 701, 759, 779
BslFI GGGAC 2 cut(s) 451, 793
BslI CCNNNNNNNGG 8 cut(s) 300, 476, 500, 566, 662, 704, 769, 803
BsmAI GTCTC 1 cut(s) 517
BsmFI GGGAC 2 cut(s) 451, 793
BsnI GGCC 8 cut(s) 330, 380, 481, 507, 565, 710, 762, 774
Bso31I GGTCTC 1 cut(s) 517
BsoBI CYCGRG 2 cut(s) 382, 482
Bsp120I GGGCCC 3 cut(s) 479, 505, 772
Bsp1286I GDGCHC 4 cut(s) 213, 483, 509, 776
Bsp13I TCCGGA 1 cut(s) 700
BspACI CCGC 9 cut(s) 432, 440, 690, 695, 711, 770, 776, 797, 851
BspANI GGCC 8 cut(s) 330, 380, 481, 507, 565, 710, 762, 774
BspCNI CTCAG 3 cut(s) 136, 136, 296
BspEI TCCGGA 1 cut(s) 700
BspHI TCATGA 1 cut(s) 908
BspT107I GGYRCC 2 cut(s) 387, 646
BspTNI GGTCTC 1 cut(s) 517
BsrBI CCGCTC 1 cut(s) 434
BsrI ACTGG 2 cut(s) 401, 530
BssECI CCNNGG 7 cut(s) 383, 482, 560, 566, 734, 803, 810
BssNI GRCGYC 1 cut(s) 659
Bst2UI CCWGG 5 cut(s) 561, 567, 735, 804, 812
Bst4CI ACNGT 3 cut(s) 275, 582, 787
BstACI GRCGYC 1 cut(s) 659
BstBAI YACGTR 1 cut(s) 842
BstC8I GCNNGC 6 cut(s) 651, 731, 764, 768, 772, 776
BstDEI CTNAG 3 cut(s) 123, 144, 283
BstF5I GGATG 2 cut(s) 62, 755
BstH2I RGCGCY 2 cut(s) 744, 905
BstHHI GCGC 2 cut(s) 743, 904
BstMAI GTCTC 1 cut(s) 517
BstMWI GCNNNNNNNGC 1 cut(s) 394
BstNI CCWGG 5 cut(s) 561, 567, 735, 804, 812
BstSCI CCNGG 9 cut(s) 481, 482, 507, 559, 565, 733, 778, 802, 810
BstSFI CTRYAG 1 cut(s) 130
BstSLI GKGCMC 3 cut(s) 483, 509, 776
BstV1I GCAGC 4 cut(s) 227, 448, 454, 484
BstV2I GAAGAC 1 cut(s) 17
BsuI GTATCC 1 cut(s) 68
BsuRI GGCC 8 cut(s) 330, 380, 481, 507, 565, 710, 762, 774
BtgZI GCGATG 1 cut(s) 573
BtsCI GGATG 2 cut(s) 62, 755
BtsIMutI CAGTG 2 cut(s) 537, 901
Cac8I GCNNGC 6 cut(s) 651, 731, 764, 768, 772, 776
CaiI CAGNNNCTG 2 cut(s) 138, 500
CciI TCATGA 1 cut(s) 908
CfoI GCGC 2 cut(s) 743, 904
Cfr9I CCCGGG 1 cut(s) 482
CseI GACGC 2 cut(s) 453, 648
Csp6I GTAC 1 cut(s) 187
CviAII CATG 1 cut(s) 909
CviQI GTAC 1 cut(s) 187
DdeI CTNAG 3 cut(s) 123, 144, 283
DrdI GACNNNNNNGTC 1 cut(s) 826
DseDI GACNNNNNNGTC 1 cut(s) 826
EaeI YGGCCR 2 cut(s) 328, 760
EciI GGCGGA 1 cut(s) 786
Eco24I GRGCYC 3 cut(s) 483, 509, 776
Eco31I GGTCTC 1 cut(s) 517
Eco47I GGWCC 2 cut(s) 308, 807
Eco57I CTGAAG 1 cut(s) 220
Eco88I CYCGRG 2 cut(s) 382, 482
EcoO109I RGGNCCY 3 cut(s) 479, 505, 807
EcoRII CCWGG 5 cut(s) 559, 565, 733, 802, 810
EcoT38I GRGCYC 3 cut(s) 483, 509, 776
FaeI CATG 1 cut(s) 912
FaiI YATR 4 cut(s) 68, 87, 669, 910
FaqI GGGAC 2 cut(s) 451, 793
FatI CATG 1 cut(s) 908
FauI CCCGC 3 cut(s) 697, 763, 783
Fnu4HI GCNGC 6 cut(s) 241, 443, 462, 498, 711, 852
FokI GGATG 2 cut(s) 49, 742
FriOI GRGCYC 3 cut(s) 483, 509, 776
Fsp4HI GCNGC 6 cut(s) 241, 443, 462, 498, 711, 852
FspBI CTAG 1 cut(s) 423
GlaI GCGC 2 cut(s) 742, 903
GluI GCNGC 6 cut(s) 241, 443, 462, 498, 711, 852
GsaI CCCAGC 2 cut(s) 497, 504
GsuI CTGGAG 1 cut(s) 852
HaeII RGCGCY 2 cut(s) 744, 905
HaeIII GGCC 8 cut(s) 330, 380, 481, 507, 565, 710, 762, 774
HapII CCGG 8 cut(s) 483, 509, 605, 644, 656, 701, 759, 779
HgaI GACGC 2 cut(s) 453, 648
HhaI GCGC 2 cut(s) 743, 904
Hin1I GRCGYC 1 cut(s) 659
Hin1II CATG 1 cut(s) 912
Hin6I GCGC 2 cut(s) 741, 902
HinP1I GCGC 2 cut(s) 741, 902
HincII GTYRAC 2 cut(s) 597, 621
HindII GTYRAC 2 cut(s) 597, 621
HindIII AAGCTT 1 cut(s) 359
HinfI GANTC 2 cut(s) 203, 281
HpaII CCGG 8 cut(s) 483, 509, 605, 644, 656, 701, 759, 779
HphI GGTGA 3 cut(s) 283, 519, 549
Hpy166II GTNNAC 4 cut(s) 115, 220, 597, 621
Hpy188I TCNGA 5 cut(s) 126, 202, 264, 286, 357
Hpy188III TCNNGA 5 cut(s) 217, 306, 701, 831, 909
Hpy8I GTNNAC 4 cut(s) 115, 220, 597, 621
Hpy99I CGWCG 3 cut(s) 218, 275, 586
HpyAV CCTTC 4 cut(s) 65, 129, 187, 867
HpyCH4III ACNGT 3 cut(s) 275, 582, 787
HpyCH4IV ACGT 1 cut(s) 841
HpyCH4V TGCA 1 cut(s) 243
HpyF10VI GCNNNNNNNGC 1 cut(s) 394
HpyF3I CTNAG 3 cut(s) 123, 144, 283
HpySE526I ACGT 1 cut(s) 841
Hsp92I GRCGYC 1 cut(s) 659
Hsp92II CATG 1 cut(s) 912
HspAI GCGC 2 cut(s) 741, 902
KflI GGGWCCC 1 cut(s) 807
Kpn2I TCCGGA 1 cut(s) 700
LmnI GCTCC 5 cut(s) 439, 619, 658, 833, 859
Lsp1109I GCAGC 4 cut(s) 227, 448, 454, 484
MaeI CTAG 1 cut(s) 423
MaeII ACGT 1 cut(s) 841
MaeIII GTNAC 2 cut(s) 787, 837
MbiI CCGCTC 1 cut(s) 434
MboII GAAGA 1 cut(s) 17
MhlI GDGCHC 4 cut(s) 213, 483, 509, 776
MlsI TGGCCA 1 cut(s) 330
MluCI AATT 2 cut(s) 80, 340
MluNI TGGCCA 1 cut(s) 330
MlyI GAGTC 2 cut(s) 197, 275
MmeI TCCRAC 2 cut(s) 656, 698
Mox20I TGGCCA 1 cut(s) 330
MroI TCCGGA 1 cut(s) 700
MscI TGGCCA 1 cut(s) 330
Msp20I TGGCCA 1 cut(s) 330
MspA1I CMGCKG 2 cut(s) 240, 497
MspI CCGG 8 cut(s) 483, 509, 605, 644, 656, 701, 759, 779
MspR9I CCNGG 9 cut(s) 483, 484, 509, 561, 567, 735, 780, 804, 812
MvaI CCWGG 5 cut(s) 561, 567, 735, 804, 812
MwoI GCNNNNNNNGC 1 cut(s) 394
NciI CCSGG 4 cut(s) 483, 484, 509, 780
NlaIII CATG 1 cut(s) 912
PagI TCATGA 1 cut(s) 908
PcsI WCGNNNNNNNCGW 2 cut(s) 276, 590
PflMI CCANNNNNTGG 1 cut(s) 500
PkrI GCNGC 6 cut(s) 242, 444, 463, 499, 712, 853
PleI GAGTC 2 cut(s) 197, 275
PpsI GAGTC 2 cut(s) 197, 275
Ppu21I YACGTR 1 cut(s) 842
PpuMI RGGWCCY 1 cut(s) 807
Psp5II RGGWCCY 1 cut(s) 807
Psp6I CCWGG 5 cut(s) 559, 565, 733, 802, 810
PspFI CCCAGC 2 cut(s) 493, 500
PspGI CCWGG 5 cut(s) 559, 565, 733, 802, 810
PspOMI GGGCCC 3 cut(s) 479, 505, 772
PspPPI RGGWCCY 1 cut(s) 807
PstNI CAGNNNCTG 2 cut(s) 138, 500
PvuII CAGCTG 2 cut(s) 240, 497
RsaI GTAC 1 cut(s) 188
RsaNI GTAC 1 cut(s) 187
SatI GCNGC 6 cut(s) 241, 443, 462, 498, 711, 852
SchI GAGTC 2 cut(s) 197, 275
ScrFI CCNGG 9 cut(s) 483, 484, 509, 561, 567, 735, 780, 804, 812
SduI GDGCHC 4 cut(s) 213, 483, 509, 776
SfcI CTRYAG 1 cut(s) 130
SinI GGWCC 2 cut(s) 308, 807
SmaI CCCGGG 1 cut(s) 484
SrfI GCCCGGGC 1 cut(s) 484
Sse9I AATT 2 cut(s) 80, 340
SsiI CCGC 9 cut(s) 432, 440, 690, 695, 711, 770, 776, 797, 851
SspMI CTAG 1 cut(s) 423
StyD4I CCNGG 9 cut(s) 481, 482, 507, 559, 565, 733, 778, 802, 810
TaaI ACNGT 3 cut(s) 275, 582, 787
TaiI ACGT 1 cut(s) 844
TaqI TCGA 3 cut(s) 270, 297, 860
TasI AATT 2 cut(s) 80, 340
TatI WGTACW 1 cut(s) 186
TauI GCSGC 2 cut(s) 713, 854
TscAI CASTG 2 cut(s) 537, 901
TseI GCWGC 4 cut(s) 240, 442, 461, 497
TspGWI ACGGA 1 cut(s) 606
TspMI CCCGGG 1 cut(s) 482
TspRI CASTG 2 cut(s) 537, 901
Van91I CCANNNNNTGG 1 cut(s) 500
VpaK11BI GGWCC 2 cut(s) 308, 807
XapI RAATTY 1 cut(s) 80
XmaI CCCGGG 1 cut(s) 482
XspI CTAG 1 cut(s) 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.