Rorug07G0232400

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0004635
Physical Location & Seq
Forward (+)
21338307 .. 21342067
3761 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0232400.1

Sequence Viewer

Length: 1221 bp
ATGGCCTCTCGCAAGGGGGTGAAACGTGCACGATCTCCAGAACCAGCCTCTTTCACAGAATCAAAGAAACTCAGAAAGGCAATTAACAACCAAACCCAGGTCGCAATGGAAATCACTGAGAAATTGCTTCTGACCGAAGGCAAGGAACAGAACATGGTTTACTCGCCACTGTCCATTCACGTGGTTCTAAGCATGATCGCTGCTGGCACAAAGGGTCTGACAAAGGACCAGTTGCTCTCCTTCCTCAAGTCCAAGTCCATCAACGAGCTTAACGACCTCGCCTCCAATGTTGCTCCGCTGGTCTTTGCCGATGGATCCCCAAGAGGTGGGCCTTGCTTGTCATTCGCCAATGGCGTTTGGGTGGAACAGTCTTGCCCAGTCAAGCCTTCTTTCAAAGATGTAGTTGACACTGCTTACAAGGCGGCCTTGAAACTAGTAGATTTTAAGACTGATTACGAACAGAGTGCATGTCCTCACATTTGTCACACCCAAGAAAGCATAAACACTCTTAACATTGAAAAGGTTCTGACTAATAAAAAAGCAAAGGCTCTTCGGTCTCTCTCTACCTCCACCGGCCACCAAACGGCGTCGAACCACTTCCTTTTGCTTCGTCTCAGCCTTGCGCAGCTGCCAGAGGCAGCCTTGCACCGTGACACGGCCACCAGCGGCGGCGGGAAGCTCCGCCCGAAGCAGCCTCACACGGCGGCGTGGCCCGTAGCAGTGGCTGCAAGTCAAACCCAACCAAGAACGAGACCGGAGCTATCGATCAAGATATCTCGAGCTAAGGCATCGAGGCCTGAAATTGAAGCTTTGACGTCGAAATCGAGCCTTGCCGGATTCTGCAAAATTCTGGAATTTTTCCGACCACCGAAGCTTTCGATCGACTTCGTGCTAGTTATGAAAGTTGTCAAGCATGATGAGAGGAAGAAGAGCAGCCCGGCCCCGACACCATTGGTGGTGGTCGGCGGCGGCTCTGCCACTAACTCCGGCGGCCCTTTCCGGCCACCTCCGGGGATCAAAAATATGGTTTCTTGGCTTTGTGTGGTCATGGGCGAAGATCCGACCGTTGGATCTTCGTATAAATGCAAAACAGTGATTAGGAAGGCGATCCGTGAGGATCCGACCGTTGGATCATCATGTAATTTCAATCCGACCGTTGGATTGTCGTTTGATTATGTTTTTGAGTTATTGGCTAAGTTAAGGTCATGTTTGATTAGGTGA

Protein Analysis

406

Amino Acids

44.05

Weight (kDa)

9.73

Isoelectric Point (pI)

45.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Serpin PF00079 30 - 153 2.7e-20 Serpin (serine protease inhibitor)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 818
Acc16I TGCGCA 1 cut(s) 624
AccB7I CCANNNNNTGG 1 cut(s) 326
AclWI GGATC 9 cut(s) 309, 322, 1022, 1052, 1078, 1102, 1112, 1125, 1138
AcoI YGGCCR 3 cut(s) 574, 657, 1001
AcsI RAATTY 2 cut(s) 846, 854
AcvI CACGTG 1 cut(s) 181
AcyI GRCGYC 2 cut(s) 587, 815
AfiI CCNNNNNNNGG 8 cut(s) 97, 326, 583, 655, 1010, 1067, 1127, 1157
AgsI TTSAA 5 cut(s) 394, 430, 518, 806, 1147
AhlI ACTAGT 1 cut(s) 433
AjnI CCWGG 1 cut(s) 96
AluBI AGCT 7 cut(s) 268, 628, 679, 760, 782, 809, 874
AluI AGCT 7 cut(s) 268, 628, 679, 760, 782, 809, 874
Alw21I GWGCWC 1 cut(s) 31
Alw26I GTCTC 3 cut(s) 561, 617, 745
Alw44I GTGCAC 1 cut(s) 27
AlwI GGATC 9 cut(s) 309, 322, 1022, 1052, 1078, 1102, 1112, 1125, 1138
AlwNI CAGNNNCTG 1 cut(s) 725
Ama87I CYCGRG 1 cut(s) 777
ApaLI GTGCAC 1 cut(s) 27
ApeKI GCWGC 7 cut(s) 200, 625, 628, 638, 691, 725, 933
ApoI RAATTY 2 cut(s) 846, 854
Asp700I GAANNNNTTC 2 cut(s) 522, 596
AspLEI GCGC 1 cut(s) 625
AspS9I GGNCC 5 cut(s) 226, 329, 711, 940, 992
AsuC2I CCSGG 2 cut(s) 938, 1011
AsuHPI GGTGA 1 cut(s) 31
AvaI CYCGRG 1 cut(s) 777
AvaII GGWCC 1 cut(s) 226
BaeGI GKGCMC 1 cut(s) 31
BamHI GGATCC 2 cut(s) 314, 1117
BbrPI CACGTG 1 cut(s) 181
Bbv12I GWGCWC 1 cut(s) 31
BbvI GCAGC 7 cut(s) 187, 615, 637, 650, 703, 712, 945
BccI CCATC 2 cut(s) 266, 305
BceAI ACGGC 3 cut(s) 600, 672, 717
BcgI CGANNNNNNTGC 2 cut(s) 446, 480
BciT130I CCWGG 1 cut(s) 98
BcnI CCSGG 2 cut(s) 938, 1011
BcoDI GTCTC 3 cut(s) 561, 617, 745
BcuI ACTAGT 1 cut(s) 433
BfaI CTAG 2 cut(s) 434, 893
Bme1390I CCNGG 3 cut(s) 98, 938, 1011
Bme18I GGWCC 1 cut(s) 226
BmeT110I CYCGRG 1 cut(s) 777
BmgT120I GGNCC 5 cut(s) 226, 329, 711, 940, 992
BmiI GGNNCC 3 cut(s) 316, 942, 1119
BmrFI CCNGG 3 cut(s) 98, 938, 1011
BmrI ACTGGG 1 cut(s) 371
BmsI GCATC 1 cut(s) 797
BmuI ACTGGG 1 cut(s) 371
BpmI CTGGAG 1 cut(s) 21
Bpu10I CCTNAGC 1 cut(s) 783
BpuEI CTTGAG 1 cut(s) 230
BpuMI CCSGG 2 cut(s) 938, 1011
Bsa29I ATCGAT 1 cut(s) 764
BsaAI YACGTR 1 cut(s) 181
BsaHI GRCGYC 2 cut(s) 587, 815
BsaI GGTCTC 2 cut(s) 561, 745
BsaJI CCNNGG 2 cut(s) 96, 1010
BsaWI WCCGGW 1 cut(s) 754
BsaXI ACNNNNNCTCC 2 cut(s) 266, 296
Bsc4I CCNNNNNNNGG 8 cut(s) 97, 326, 583, 655, 1010, 1067, 1127, 1157
Bse118I RCCGGY 1 cut(s) 572
Bse1I ACTGG 2 cut(s) 229, 377
Bse3DI GCAATG 1 cut(s) 111
BseBI CCWGG 1 cut(s) 98
BseCI ATCGAT 1 cut(s) 764
BseDI CCNNGG 2 cut(s) 96, 1010
BseLI CCNNNNNNNGG 8 cut(s) 97, 326, 583, 655, 1010, 1067, 1127, 1157
BseMI GCAATG 1 cut(s) 111
BseMII CTCAG 3 cut(s) 85, 108, 628
BseNI ACTGG 2 cut(s) 229, 377
BseSI GKGCMC 1 cut(s) 31
BseXI GCAGC 7 cut(s) 187, 615, 637, 650, 703, 712, 945
Bsh1285I CGRYCG 4 cut(s) 882, 1065, 1125, 1155
BshVI ATCGAT 1 cut(s) 764
BsiEI CGRYCG 4 cut(s) 882, 1065, 1125, 1155
BsiHKAI GWGCWC 1 cut(s) 31
BsiHKCI CYCGRG 1 cut(s) 777
BsiSI CCGG 7 cut(s) 573, 755, 834, 938, 987, 1000, 1010
BslI CCNNNNNNNGG 8 cut(s) 97, 326, 583, 655, 1010, 1067, 1127, 1157
BsmAI GTCTC 3 cut(s) 561, 617, 745
BsmBI CGTCTC 1 cut(s) 617
Bso31I GGTCTC 2 cut(s) 561, 745
BsoBI CYCGRG 1 cut(s) 777
Bsp1286I GDGCHC 1 cut(s) 31
BspCNI CTCAG 3 cut(s) 84, 109, 627
BspDI ATCGAT 1 cut(s) 764
BspLI GGNNCC 3 cut(s) 316, 942, 1119
BspPI GGATC 9 cut(s) 309, 322, 1022, 1052, 1078, 1102, 1112, 1125, 1138
BspQI GCTCTTC 2 cut(s) 555, 923
BspTNI GGTCTC 2 cut(s) 561, 745
BsrDI GCAATG 1 cut(s) 111
BsrFI RCCGGY 1 cut(s) 572
BsrI ACTGG 2 cut(s) 229, 377
BssAI RCCGGY 1 cut(s) 572
BssECI CCNNGG 2 cut(s) 96, 1010
BssNI GRCGYC 2 cut(s) 587, 815
Bst2UI CCWGG 1 cut(s) 98
Bst4CI ACNGT 7 cut(s) 171, 369, 650, 1066, 1093, 1126, 1156
Bst6I CTCTTC 2 cut(s) 555, 923
BstACI GRCGYC 2 cut(s) 587, 815
BstAPI GCANNNNNTGC 1 cut(s) 725
BstBAI YACGTR 1 cut(s) 181
BstC8I GCNNGC 1 cut(s) 205
BstDEI CTNAG 6 cut(s) 71, 117, 188, 614, 783, 1194
BstHHI GCGC 1 cut(s) 625
BstMAI GTCTC 3 cut(s) 561, 617, 745
BstMCI CGRYCG 4 cut(s) 882, 1065, 1125, 1155
BstMWI GCNNNNNNNGC 2 cut(s) 419, 725
BstNI CCWGG 1 cut(s) 98
BstNSI RCATGY 1 cut(s) 471
BstSCI CCNGG 3 cut(s) 96, 936, 1009
BstSLI GKGCMC 1 cut(s) 31
BstV1I GCAGC 7 cut(s) 187, 615, 637, 650, 703, 712, 945
BstX2I RGATCY 4 cut(s) 314, 1057, 1070, 1117
BstXI CCANNNNNNTGG 1 cut(s) 181
BstYI RGATCY 4 cut(s) 314, 1057, 1070, 1117
Bsu15I ATCGAT 1 cut(s) 764
BsuTUI ATCGAT 1 cut(s) 764
BtsI GCAGTG 2 cut(s) 408, 726
BtsIMutI CAGTG 5 cut(s) 114, 167, 408, 726, 1098
Cac8I GCNNGC 1 cut(s) 205
CaiI CAGNNNCTG 1 cut(s) 725
CfoI GCGC 1 cut(s) 625
Cfr10I RCCGGY 1 cut(s) 572
Cfr13I GGNCC 5 cut(s) 226, 329, 711, 940, 992
ClaI ATCGAT 1 cut(s) 764
CseI GACGC 1 cut(s) 576
CviAII CATG 7 cut(s) 154, 193, 468, 914, 1048, 1137, 1206
DdeI CTNAG 6 cut(s) 71, 117, 188, 614, 783, 1194
EaeI YGGCCR 3 cut(s) 574, 657, 1001
Eam1104I CTCTTC 2 cut(s) 555, 923
EarI CTCTTC 2 cut(s) 555, 923
EciI GGCGGA 1 cut(s) 671
Eco147I AGGCCT 1 cut(s) 796
Eco31I GGTCTC 2 cut(s) 561, 745
Eco32I GATATC 1 cut(s) 774
Eco47I GGWCC 1 cut(s) 226
Eco72I CACGTG 1 cut(s) 181
Eco88I CYCGRG 1 cut(s) 777
EcoRII CCWGG 1 cut(s) 96
EcoRV GATATC 1 cut(s) 774
Esp3I CGTCTC 1 cut(s) 617
FaeI CATG 7 cut(s) 157, 196, 471, 917, 1051, 1140, 1209
FalI AAGNNNNNCTT 2 cut(s) 410, 442
FatI CATG 7 cut(s) 153, 192, 467, 913, 1047, 1136, 1205
FauI CCCGC 1 cut(s) 665
FspBI CTAG 2 cut(s) 434, 893
FspI TGCGCA 1 cut(s) 624
GlaI GCGC 1 cut(s) 624
GsuI CTGGAG 1 cut(s) 21
HapII CCGG 7 cut(s) 573, 755, 834, 938, 987, 1000, 1010
HgaI GACGC 1 cut(s) 576
HhaI GCGC 1 cut(s) 625
Hin1I GRCGYC 2 cut(s) 587, 815
Hin1II CATG 7 cut(s) 157, 196, 471, 917, 1051, 1140, 1209
Hin6I GCGC 1 cut(s) 623
HinP1I GCGC 1 cut(s) 623
HincII GTYRAC 1 cut(s) 406
HindII GTYRAC 1 cut(s) 406
HindIII AAGCTT 2 cut(s) 807, 872
HinfI GANTC 2 cut(s) 59, 837
HpaII CCGG 7 cut(s) 573, 755, 834, 938, 987, 1000, 1010
HphI GGTGA 1 cut(s) 31
Hpy166II GTNNAC 3 cut(s) 29, 160, 406
Hpy188I TCNGA 8 cut(s) 74, 132, 219, 528, 863, 1062, 1122, 1152
Hpy188III TCNNGA 4 cut(s) 38, 769, 777, 851
Hpy8I GTNNAC 3 cut(s) 29, 160, 406
Hpy99I CGWCG 2 cut(s) 592, 820
HpyAV CCTTC 4 cut(s) 131, 250, 396, 1096
HpyCH4III ACNGT 7 cut(s) 171, 369, 650, 1066, 1093, 1126, 1156
HpyCH4IV ACGT 3 cut(s) 25, 180, 815
HpyCH4V TGCA 6 cut(s) 29, 467, 646, 728, 843, 1086
HpyF10VI GCNNNNNNNGC 2 cut(s) 419, 725
HpyF3I CTNAG 6 cut(s) 71, 117, 188, 614, 783, 1194
HpySE526I ACGT 3 cut(s) 25, 180, 815
Hsp92I GRCGYC 2 cut(s) 587, 815
Hsp92II CATG 7 cut(s) 157, 196, 471, 917, 1051, 1140, 1209
HspAI GCGC 1 cut(s) 623
LguI GCTCTTC 2 cut(s) 555, 923
LmnI GCTCC 3 cut(s) 298, 684, 757
Lsp1109I GCAGC 7 cut(s) 187, 615, 637, 650, 703, 712, 945
LweI GCATC 1 cut(s) 797
MaeI CTAG 2 cut(s) 434, 893
MaeII ACGT 3 cut(s) 25, 180, 815
MaeIII GTNAC 2 cut(s) 482, 650
MboII GAAGA 5 cut(s) 542, 937, 940, 1065, 1067
MflI RGATCY 4 cut(s) 314, 1057, 1070, 1117
MhlI GDGCHC 1 cut(s) 31
MluCI AATT 6 cut(s) 81, 122, 801, 846, 854, 1141
MmeI TCCRAC 7 cut(s) 886, 1048, 1085, 1108, 1138, 1145, 1175
MroXI GAANNNNTTC 2 cut(s) 522, 596
MseI TTAA 5 cut(s) 84, 270, 444, 510, 1199
MslI CAYNNNNRTG 1 cut(s) 179
MspA1I CMGCKG 3 cut(s) 298, 628, 666
MspI CCGG 7 cut(s) 573, 755, 834, 938, 987, 1000, 1010
MspR9I CCNGG 3 cut(s) 98, 938, 1011
MvaI CCWGG 1 cut(s) 98
MwoI GCNNNNNNNGC 2 cut(s) 419, 725
NciI CCSGG 2 cut(s) 938, 1011
NlaIII CATG 7 cut(s) 157, 196, 471, 917, 1051, 1140, 1209
NlaIV GGNNCC 3 cut(s) 316, 942, 1119
NmuCI GTSAC 2 cut(s) 482, 650
NsbI TGCGCA 1 cut(s) 624
NspI RCATGY 1 cut(s) 471
PaeR7I CTCGAG 1 cut(s) 777
PceI AGGCCT 1 cut(s) 796
PciSI GCTCTTC 2 cut(s) 555, 923
PcsI WCGNNNNNNNCGW 3 cut(s) 270, 351, 821
PdmI GAANNNNTTC 2 cut(s) 522, 596
PfeI GAWTC 2 cut(s) 59, 837
PflMI CCANNNNNTGG 1 cut(s) 326
Ple19I CGATCG 1 cut(s) 882
PmaCI CACGTG 1 cut(s) 181
PmlI CACGTG 1 cut(s) 181
Ppu21I YACGTR 1 cut(s) 181
Psp6I CCWGG 1 cut(s) 96
PspCI CACGTG 1 cut(s) 181
PspGI CCWGG 1 cut(s) 96
PspN4I GGNNCC 3 cut(s) 316, 942, 1119
PspPI GGNCC 5 cut(s) 226, 329, 711, 940, 992
PsrI GAACNNNNNNTAC 2 cut(s) 143, 175
PstNI CAGNNNCTG 1 cut(s) 725
PsuI RGATCY 4 cut(s) 314, 1057, 1070, 1117
PvuI CGATCG 1 cut(s) 882
PvuII CAGCTG 1 cut(s) 628
RseI CAYNNNNRTG 1 cut(s) 179
SapI GCTCTTC 2 cut(s) 555, 923
SaqAI TTAA 5 cut(s) 84, 270, 444, 510, 1199
Sau96I GGNCC 5 cut(s) 226, 329, 711, 940, 992
ScrFI CCNGG 3 cut(s) 98, 938, 1011
SduI GDGCHC 1 cut(s) 31
SfaNI GCATC 1 cut(s) 797
Sfr274I CTCGAG 1 cut(s) 777
SinI GGWCC 1 cut(s) 226
SlaI CTCGAG 1 cut(s) 777
SmiMI CAYNNNNRTG 1 cut(s) 179
SmlI CTYRAG 2 cut(s) 245, 777
SmoI CTYRAG 2 cut(s) 245, 777
SpeI ACTAGT 1 cut(s) 433
Sse9I AATT 6 cut(s) 81, 122, 801, 846, 854, 1141
SseBI AGGCCT 1 cut(s) 796
SspMI CTAG 2 cut(s) 434, 893
StuI AGGCCT 1 cut(s) 796
StyD4I CCNGG 3 cut(s) 96, 936, 1009
TaaI ACNGT 7 cut(s) 171, 369, 650, 1066, 1093, 1126, 1156
TaiI ACGT 3 cut(s) 28, 183, 818
TaqI TCGA 8 cut(s) 590, 764, 778, 791, 818, 824, 878, 882
TaqII GACCGA 2 cut(s) 149, 543
TasI AATT 6 cut(s) 81, 122, 801, 846, 854, 1141
TauI GCSGC 7 cut(s) 425, 669, 672, 707, 969, 972, 993
TfiI GAWTC 2 cut(s) 59, 837
Tru1I TTAA 5 cut(s) 84, 270, 444, 510, 1199
Tru9I TTAA 5 cut(s) 84, 270, 444, 510, 1199
TscAI CASTG 5 cut(s) 121, 174, 415, 726, 1098
TseFI GTSAC 2 cut(s) 482, 650
TseI GCWGC 7 cut(s) 200, 625, 628, 638, 691, 725, 933
Tsp45I GTSAC 2 cut(s) 482, 650
TspDTI ATGAA 1 cut(s) 914
TspGWI ACGGA 1 cut(s) 1100
TspRI CASTG 5 cut(s) 121, 174, 415, 726, 1098
Van91I CCANNNNNTGG 1 cut(s) 326
VneI GTGCAC 1 cut(s) 27
VpaK11BI GGWCC 1 cut(s) 226
XapI RAATTY 2 cut(s) 846, 854
XceI RCATGY 1 cut(s) 471
XhoI CTCGAG 1 cut(s) 777
XmnI GAANNNNTTC 2 cut(s) 522, 596
XspI CTAG 2 cut(s) 434, 893
ZraI GACGTC 1 cut(s) 816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.