FvH4_4g02170

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001889
Physical Location & Seq
Reverse (-)
2027418 .. 2031345
3928 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g02170.t1

Sequence Viewer

Length: 2460 bp
ATGGCCAAACTAAGAAGCATTGTCTTCCTCTTTTTCTCATGTTTCTGCATGCATTCTCTTTCTTCTTCTCATGCACAAACACATAATGCTACACTCAAGCAAGGCCAACAGCTAAGCGACCAGGATGGCGATTATCTTGTTTCCAAAAATGGTTTGTTCAAGTTAGGCTTCTTTAGTCCAGGCAACTCAAGCATTTTCGGTGCTACAATTAACCGTTACTTGGGAGTATGGTACTACAAACTACCAAATGATCCAGATGCAGTGTGGGTAGCCAATCCAGAAACTCCGATCATTGATACATCAGGGGTATTTGCATTAGCTAGTGATGCAACATTGAAGCTCATTCATGGTGGAGGCCAAGTATCTGTCTCAGATCCTAATAAAACAGTTTCTGGAAATGTGACTCTCTCCTTATTAGATACTGGAAATTTGGTCCTGCGAGAAGTACTAGCTACTAATGGAACAGCAGGAAATGTTATATGGGAAAGCTTTGACCATCCAACCAACACATTGTTACCTGGAATGAAATTGGGCATGAATGTCAAGGCTGGAGAGAATTGGACTATTTCTTCCTGGTTCAGCGATCAAATACCTGCACCAGGTGCTTTCAGGCTTGGCATTGATCCTGGTAGCAACAACCAATTGATCATCTGGCGACGAGATGAAGTATACTGGAGCAGTGGGATCTGGGAAAATGGATCTTTTCAAAATGCTCATGAAATGACAAGAAGGGTTGATCTGTTTGAGTTTACTTTTGTCTCAAACAAGGAAGATAAGTACTTTTCTTATACTGCTAAAAATAGGTCTACTATCTCAAGGTGGGAACTGAATGCTTGGGGCCAAATCATGCAGTACATTTTAACTGTCAACGGTACTACATGGGAGAGAACAGAAGCTAGTGCATGCACATTCAACTTGAATAATCCTGGAGCCATGTGCATAGAGGAGAAGCCCTCAGAATGCAGGAATGACTCGGATGGACTTGTACCAGTAAGAGGCTATTTTGATGGAACTGAAATTCTAAAGCATGATAACAATACTAACATGACCATTAGTGATTGCCATGCCACTTGCTGGAGTGACTGCTCCTGCATTGGTTTCAAAAGTATTTATTCCAATGGGACTGGGTGTGTATTTATGAGTGGAGCAGCAAGATTTGTCGCCAATGATTACTTTGATGCTACTTATGTCCTTAACATATTTAATAATAGCAAAGAAGGAAGCAAAATGAGCGATTTAACAGAAGATATAAAGAAAAAATGGTGGATATGGTGCATCATAGGTCTTATATTTTTCATGGCATTGCTAGTATGTTACTTATGCTACAAAAAGAAAAGAAAGCTCAGACTCATGAAACACACTGGCCAGGAACAAGGATTAGCAGAGTTGAGATCTCAATTTACCAGATTCGACCACATGTACAAGCTCAAAATAGGTGAAAGAATAGGACAGAACTTTGGGATGTTCAGTTTTTCAGAAGTAGTGGCTGCAACAGATAACTTCTCATCTGCCAGCAAGCTAGGACAGGGTGGTTTTGGACCTGTTTATAAGGGGGTATTACCAGATGGGGAACAGGTAGGGATAAAGAGACTCGCCAGAAGTTCAGGACAAGGGCTAGAAGAATTCATGAATGAGATTACACTTATAGCTGAGCTTCAACATAGTAATCTTGTTAGGCTGTTGGGTTGTTGCATTCAAGGAGAAGAAAAGATTCTGATTTACGAATTTATGGTGAATAAGAGCTTGGATGCGTTCCTATTTGACTCCACTAGAAGGAGGCAATTGGACTGGCAAAGACGGGTAAGCATCATCGAAGGAATTGCACAAGGACTTCTCTATCTTCATAAGCATTCTAGAGTTAGAGTCATACACAGAGATTTGAAAGCTAGTAACATTCTGCTTGATGAAAATATGAACCCTAAGATCTCAGACTTTGGAATGGCCAGGATTTTCGGACAGAATGAATCTATGGCAAATACAGTTAGAGTTGTTGGAACATACGGTTACATGTCTCCAGAGTATGCCATGATGGGCGTTTTTTCTGAAAAGTCTGATGTTTATAGTTTTGGAGTTCTACTGTTGGAGATTATAAGTGGCAAAAAGAACACTAATTTTCTTTCATCTACTGGTCTCTGCCTTATCGAACTTGCCTGGGAATTGTGGAAACAAGGACATACTCTGGAGATAAAGGACACATCACTTGACTTGGGGTCTAAAGAGGAAGTTTTAAAATTTATTCAGGTAGGTCTCCTATGTGTTCAAGAGCATGCAGAGGATAGAGCTAACATGTCAGAAGTAATATCAATGCTTACAAGTGATGTCACACATTTGCCTAATCCAAAGCAACCTGCATACACTATTTCAAGAAGTGAAGCTGGGTCAACTACATTACCTGCAGATACAAGTTCAAATACGGCTTCAGTAAATTATGAATCCATGACTGTGATGGATGGTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

820

Amino Acids

91.63

Weight (kDa)

6.07

Isoelectric Point (pI)

37.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 88 - 192 9.1e-27 D-mannose binding lectin
S_locus_glycop PF00954 225 - 321 1.3e-07 S-locus glycoprotein domain
Pkinase PF00069 502 - 704 1.2e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 504 - 769 1.1e-48 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 772 - 819 5.2e-07 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1548, 2090
Acc36I ACCTGC 3 cut(s) 601, 2356, 2401
AccB7I CCANNNNNTGG 1 cut(s) 1074
AccI GTMKAC 2 cut(s) 669, 806
AclWI GGATC 5 cut(s) 245, 368, 617, 692, 706
AcoI YGGCCR 3 cut(s) 3, 1363, 1941
AcsI RAATTY 5 cut(s) 427, 1017, 1622, 1724, 2231
AcuI CTGAAG 1 cut(s) 2403
AdeI CACNNNGTG 1 cut(s) 602
AfaI GTAC 7 cut(s) 233, 447, 779, 854, 874, 987, 1421
AfiI CCNNNNNNNGG 5 cut(s) 220, 599, 995, 1074, 1773
AflIII ACRYGT 3 cut(s) 1416, 2007, 2286
AhdI GACNNNNNGTC 1 cut(s) 2209
AjuI GAANNNNNNNTTGG 2 cut(s) 1727, 1759
Alw26I GTCTC 6 cut(s) 373, 763, 1582, 2016, 2135, 2252
AlwI GGATC 5 cut(s) 245, 368, 617, 692, 706
AlwNI CAGNNNCTG 1 cut(s) 392
AoxI GGCC 6 cut(s) 3, 103, 355, 838, 1363, 1941
ApeKI GCWGC 2 cut(s) 1148, 1487
ApoI RAATTY 5 cut(s) 427, 1017, 1622, 1724, 2231
Asp700I GAANNNNTTC 1 cut(s) 1710
AspS9I GGNCC 3 cut(s) 433, 838, 1538
AsuHPI GGTGA 2 cut(s) 1448, 1744
AvaII GGWCC 2 cut(s) 433, 1538
BalI TGGCCA 3 cut(s) 5, 1365, 1943
BbsI GAAGAC 1 cut(s) 16
BbvI GCAGC 2 cut(s) 1160, 1474
BccI CCATC 8 cut(s) 119, 504, 971, 1001, 1559, 2023, 2440, 2444
BceAI ACGGC 1 cut(s) 2430
BcgI CGANNNNNNTGC 2 cut(s) 1802, 1836
BclI TGATCA 1 cut(s) 645
BcoDI GTCTC 6 cut(s) 373, 763, 1582, 2016, 2135, 2252
BfaI CTAG 9 cut(s) 321, 449, 897, 1307, 1520, 1616, 1770, 1854, 1887
BfmI CTRYAG 1 cut(s) 2394
BfuAI ACCTGC 3 cut(s) 601, 2356, 2401
BglII AGATCT 2 cut(s) 1391, 1923
BisI GCNGC 2 cut(s) 1149, 1488
BlpI GCTNAGC 2 cut(s) 113, 1650
BlsI GCNGC 2 cut(s) 1150, 1489
BmcAI AGTACT 2 cut(s) 447, 779
Bme18I GGWCC 2 cut(s) 433, 1538
BmeRI GACNNNNNGTC 1 cut(s) 2209
BmgT120I GGNCC 3 cut(s) 433, 838, 1538
BmiI GGNNCC 2 cut(s) 839, 931
BmrI ACTGGG 1 cut(s) 1134
BmsI GCATC 6 cut(s) 247, 316, 1168, 1284, 1738, 1815
BmuI ACTGGG 1 cut(s) 1134
BpiI GAAGAC 1 cut(s) 16
BplI GAGNNNNNCTC 2 cut(s) 938, 970
BpmI CTGGAG 6 cut(s) 570, 694, 948, 1096, 1998, 2201
Bpu1102I GCTNAGC 2 cut(s) 113, 1650
BpuEI CTTGAG 3 cut(s) 80, 172, 799
BsaI GGTCTC 2 cut(s) 2135, 2252
BsaJI CCNNGG 1 cut(s) 2151
BsaXI ACNNNNNCTCC 2 cut(s) 345, 375
Bsc4I CCNNNNNNNGG 5 cut(s) 220, 599, 995, 1074, 1773
Bse1I ACTGG 7 cut(s) 427, 677, 989, 1129, 1366, 1793, 2131
Bse3DI GCAATG 1 cut(s) 1301
BseDI CCNNGG 1 cut(s) 2151
BseGI GGATG 6 cut(s) 130, 496, 982, 1467, 1753, 2455
BseLI CCNNNNNNNGG 5 cut(s) 220, 599, 995, 1074, 1773
BseMI GCAATG 1 cut(s) 1301
BseMII CTCAG 5 cut(s) 384, 969, 1357, 1641, 1941
BseNI ACTGG 7 cut(s) 427, 677, 989, 1129, 1366, 1793, 2131
BseRI GAGGAG 1 cut(s) 959
BseXI GCAGC 2 cut(s) 1160, 1474
BseYI CCCAGC 1 cut(s) 2375
BsgI GTGCAG 1 cut(s) 579
BshFI GGCC 6 cut(s) 5, 105, 357, 840, 1365, 1943
BslFI GGGAC 1 cut(s) 1135
BslI CCNNNNNNNGG 5 cut(s) 220, 599, 995, 1074, 1773
BsmAI GTCTC 6 cut(s) 373, 763, 1582, 2016, 2135, 2252
BsmFI GGGAC 1 cut(s) 1135
BsmI GAATGC 5 cut(s) 52, 835, 965, 1692, 1849
BsnI GGCC 6 cut(s) 5, 105, 357, 840, 1365, 1943
Bso31I GGTCTC 2 cut(s) 2135, 2252
Bsp1407I TGTACA 1 cut(s) 1419
Bsp1720I GCTNAGC 2 cut(s) 113, 1650
BspANI GGCC 6 cut(s) 5, 105, 357, 840, 1365, 1943
BspCNI CTCAG 5 cut(s) 383, 968, 1356, 1642, 1940
BspHI TCATGA 3 cut(s) 715, 1350, 1626
BspLI GGNNCC 2 cut(s) 839, 931
BspMAI CTGCAG 1 cut(s) 2398
BspMI ACCTGC 3 cut(s) 601, 2356, 2401
BspPI GGATC 5 cut(s) 245, 368, 617, 692, 706
BspTNI GGTCTC 2 cut(s) 2135, 2252
BsrDI GCAATG 1 cut(s) 1301
BsrGI TGTACA 1 cut(s) 1419
BsrI ACTGG 7 cut(s) 427, 677, 989, 1129, 1366, 1793, 2131
BssECI CCNNGG 1 cut(s) 2151
BssNAI GTATAC 1 cut(s) 670
Bst1107I GTATAC 1 cut(s) 670
Bst4CI ACNGT 8 cut(s) 215, 388, 865, 872, 1981, 2003, 2079, 2443
BstAPI GCANNNNNTGC 1 cut(s) 602
BstAUI TGTACA 1 cut(s) 1419
BstC8I GCNNGC 5 cut(s) 50, 904, 1513, 1517, 2268
BstDEI CTNAG 8 cut(s) 11, 113, 370, 955, 1343, 1650, 1920, 1927
BstENI CCTNNNNNAGG 1 cut(s) 597
BstF5I GGATG 6 cut(s) 130, 496, 982, 1467, 1753, 2455
BstMAI GTCTC 6 cut(s) 373, 763, 1582, 2016, 2135, 2252
BstMWI GCNNNNNNNGC 4 cut(s) 189, 326, 602, 1230
BstNSI RCATGY 6 cut(s) 52, 906, 1420, 2011, 2270, 2290
BstSFI CTRYAG 1 cut(s) 2394
BstV1I GCAGC 2 cut(s) 1160, 1474
BstV2I GAAGAC 1 cut(s) 16
BstX2I RGATCY 5 cut(s) 373, 684, 698, 1391, 1923
BstYI RGATCY 5 cut(s) 373, 684, 698, 1391, 1923
BstZ17I GTATAC 1 cut(s) 670
BsuRI GGCC 6 cut(s) 5, 105, 357, 840, 1365, 1943
BtsCI GGATG 6 cut(s) 130, 496, 982, 1467, 1753, 2455
BtsI GCAGTG 2 cut(s) 267, 685
BtsIMutI CAGTG 3 cut(s) 267, 685, 1359
BveI ACCTGC 3 cut(s) 601, 2356, 2401
Cac8I GCNNGC 5 cut(s) 50, 904, 1513, 1517, 2268
CaiI CAGNNNCTG 1 cut(s) 392
CciI TCATGA 3 cut(s) 715, 1350, 1626
Cfr13I GGNCC 3 cut(s) 433, 838, 1538
CsiI ACCWGGT 1 cut(s) 598
Csp6I GTAC 7 cut(s) 232, 446, 778, 853, 873, 986, 1420
CviQI GTAC 7 cut(s) 232, 446, 778, 853, 873, 986, 1420
DdeI CTNAG 8 cut(s) 11, 113, 370, 955, 1343, 1650, 1920, 1927
DraI TTTAAA 1 cut(s) 2229
DraIII CACNNNGTG 1 cut(s) 602
DriI GACNNNNNGTC 1 cut(s) 2209
EaeI YGGCCR 3 cut(s) 3, 1363, 1941
Eam1105I GACNNNNNGTC 1 cut(s) 2209
Eco31I GGTCTC 2 cut(s) 2135, 2252
Eco47I GGWCC 2 cut(s) 433, 1538
Eco57I CTGAAG 1 cut(s) 2403
EcoNI CCTNNNNNAGG 1 cut(s) 597
EcoRI GAATTC 1 cut(s) 1622
EcoT22I ATGCAT 1 cut(s) 54
FalI AAGNNNNNCTT 2 cut(s) 152, 184
FaqI GGGAC 1 cut(s) 1135
FbaI TGATCA 1 cut(s) 645
FblI GTMKAC 2 cut(s) 669, 806
Fnu4HI GCNGC 2 cut(s) 1149, 1488
FokI GGATG 5 cut(s) 137, 483, 989, 1474, 1760
Fsp4HI GCNGC 2 cut(s) 1149, 1488
FspBI CTAG 9 cut(s) 321, 449, 897, 1307, 1520, 1616, 1770, 1854, 1887
GluI GCNGC 2 cut(s) 1149, 1488
GsaI CCCAGC 1 cut(s) 2379
GsuI CTGGAG 6 cut(s) 570, 694, 948, 1096, 1998, 2201
HaeIII GGCC 6 cut(s) 5, 105, 357, 840, 1365, 1943
HincII GTYRAC 2 cut(s) 868, 2382
HindII GTYRAC 2 cut(s) 868, 2382
HindIII AAGCTT 1 cut(s) 487
HphI GGTGA 2 cut(s) 1448, 1744
Hpy166II GTNNAC 5 cut(s) 670, 750, 807, 868, 2382
Hpy8I GTNNAC 5 cut(s) 670, 750, 807, 868, 2382
Hpy99I CGWCG 1 cut(s) 660
HpyAV CCTTC 4 cut(s) 723, 1211, 1767, 1808
HpyCH4III ACNGT 8 cut(s) 215, 388, 865, 872, 1981, 2003, 2079, 2443
HpyF10VI GCNNNNNNNGC 4 cut(s) 189, 326, 602, 1230
HpyF3I CTNAG 8 cut(s) 11, 113, 370, 955, 1343, 1650, 1920, 1927
Ksp22I TGATCA 1 cut(s) 645
LmnI GCTCC 4 cut(s) 675, 929, 1091, 1145
Lsp1109I GCAGC 2 cut(s) 1160, 1474
LweI GCATC 6 cut(s) 247, 316, 1168, 1284, 1738, 1815
MabI ACCWGGT 1 cut(s) 598
MaeI CTAG 9 cut(s) 321, 449, 897, 1307, 1520, 1616, 1770, 1854, 1887
MaeIII GTNAC 8 cut(s) 215, 400, 513, 1079, 1313, 1889, 2003, 2320
MboII GAAGA 9 cut(s) 16, 54, 57, 561, 782, 1256, 1631, 1715, 1832
MfeI CAATTG 2 cut(s) 641, 1781
MflI RGATCY 5 cut(s) 373, 684, 698, 1391, 1923
MlsI TGGCCA 3 cut(s) 5, 1365, 1943
MluNI TGGCCA 3 cut(s) 5, 1365, 1943
MlyI GAGTC 6 cut(s) 397, 965, 1341, 1584, 1757, 1872
MmeI TCCRAC 3 cut(s) 524, 1972, 2061
MnlI CCTC 8 cut(s) 38, 347, 937, 964, 989, 1770, 2212, 2266
Mox20I TGGCCA 3 cut(s) 5, 1365, 1943
Mph1103I ATGCAT 1 cut(s) 54
MroXI GAANNNNTTC 1 cut(s) 1710
MscI TGGCCA 3 cut(s) 5, 1365, 1943
MseI TTAA 6 cut(s) 210, 860, 1194, 1203, 1238, 2228
MslI CAYNNNNRTG 1 cut(s) 2441
Msp20I TGGCCA 3 cut(s) 5, 1365, 1943
MunI CAATTG 2 cut(s) 641, 1781
Mva1269I GAATGC 5 cut(s) 52, 835, 965, 1692, 1849
MwoI GCNNNNNNNGC 4 cut(s) 189, 326, 602, 1230
NlaIV GGNNCC 2 cut(s) 839, 931
NmuCI GTSAC 3 cut(s) 400, 1079, 2320
NsiI ATGCAT 1 cut(s) 54
NspI RCATGY 6 cut(s) 52, 906, 1420, 2011, 2270, 2290
PaeI GCATGC 3 cut(s) 52, 906, 2270
PagI TCATGA 3 cut(s) 715, 1350, 1626
PciI ACATGT 3 cut(s) 1416, 2007, 2286
PctI GAATGC 5 cut(s) 52, 835, 965, 1692, 1849
PdmI GAANNNNTTC 1 cut(s) 1710
PfeI GAWTC 4 cut(s) 1407, 1711, 1964, 2432
PflMI CCANNNNNTGG 1 cut(s) 1074
PfoI TCCNGGA 1 cut(s) 925
PkrI GCNGC 2 cut(s) 1150, 1489
PleI GAGTC 6 cut(s) 397, 965, 1341, 1584, 1757, 1871
PpsI GAGTC 6 cut(s) 397, 965, 1341, 1584, 1757, 1871
PscI ACATGT 3 cut(s) 1416, 2007, 2286
PsiI TTATAA 2 cut(s) 1548, 2090
PspFI CCCAGC 1 cut(s) 2375
PspN4I GGNNCC 2 cut(s) 839, 931
PspPI GGNCC 3 cut(s) 433, 838, 1538
PstI CTGCAG 1 cut(s) 2398
PstNI CAGNNNCTG 1 cut(s) 392
PsuI RGATCY 5 cut(s) 373, 684, 698, 1391, 1923
RsaI GTAC 7 cut(s) 233, 447, 779, 854, 874, 987, 1421
RsaNI GTAC 7 cut(s) 232, 446, 778, 853, 873, 986, 1420
RseI CAYNNNNRTG 1 cut(s) 2441
SaqAI TTAA 6 cut(s) 210, 860, 1194, 1203, 1238, 2228
SatI GCNGC 2 cut(s) 1149, 1488
Sau96I GGNCC 3 cut(s) 433, 838, 1538
ScaI AGTACT 2 cut(s) 447, 779
SchI GAGTC 6 cut(s) 397, 965, 1341, 1584, 1757, 1872
SexAI ACCWGGT 1 cut(s) 598
SfaNI GCATC 6 cut(s) 247, 316, 1168, 1284, 1738, 1815
SfcI CTRYAG 1 cut(s) 2394
SinI GGWCC 2 cut(s) 433, 1538
SmiMI CAYNNNNRTG 1 cut(s) 2441
SmlI CTYRAG 3 cut(s) 95, 187, 814
SmoI CTYRAG 3 cut(s) 95, 187, 814
SphI GCATGC 3 cut(s) 52, 906, 2270
SspMI CTAG 9 cut(s) 321, 449, 897, 1307, 1520, 1616, 1770, 1854, 1887
TaaI ACNGT 8 cut(s) 215, 388, 865, 872, 1981, 2003, 2079, 2443
TaqI TCGA 3 cut(s) 1410, 1812, 2142
TatI WGTACW 4 cut(s) 445, 777, 852, 1419
TfiI GAWTC 4 cut(s) 1407, 1711, 1964, 2432
Tru1I TTAA 6 cut(s) 210, 860, 1194, 1203, 1238, 2228
Tru9I TTAA 6 cut(s) 210, 860, 1194, 1203, 1238, 2228
TscAI CASTG 3 cut(s) 267, 685, 1366
TseFI GTSAC 3 cut(s) 400, 1079, 2320
TseI GCWGC 2 cut(s) 1148, 1487
Tsp45I GTSAC 3 cut(s) 400, 1079, 2320
TspRI CASTG 3 cut(s) 267, 685, 1366
Van91I CCANNNNNTGG 1 cut(s) 1074
VpaK11BI GGWCC 2 cut(s) 433, 1538
XagI CCTNNNNNAGG 1 cut(s) 597
XapI RAATTY 5 cut(s) 427, 1017, 1622, 1724, 2231
XbaI TCTAGA 1 cut(s) 1853
XceI RCATGY 6 cut(s) 52, 906, 1420, 2011, 2270, 2290
XcmI CCANNNNNNNNNTGG 2 cut(s) 261, 2443
XmiI GTMKAC 2 cut(s) 669, 806
XmnI GAANNNNTTC 1 cut(s) 1710
XspI CTAG 9 cut(s) 321, 449, 897, 1307, 1520, 1616, 1770, 1854, 1887
ZrmI AGTACT 2 cut(s) 447, 779
Zsp2I ATGCAT 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.