Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0001889

Physical Location & Seq

Genomic Coordinates Reverse (-)

3762689 .. 3764077

Gene Structure 1389 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1047300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 957 bp

ATGTTCAGTTTTCCTGAATTAGTGACTGCAACAGATGACTTCTCATTTGCCCGAAGACTAGGAGAGGGTGGTTTTGGACCGGTCTACAAGGGTGTATTACCAGACGGGCAACAGGTAGCAGTAAAGAGACTTGCAAGACATTCAGGACAAGGATTGGAAGAATTCATGAATGAGATAACACTTATAGCTGAACTTCAACATAGTAATCTTGTTCGGCTTTTGGGTTGTTGCATTCAAGGAGAAGAGAAAATTCTGATCTATGAATACCTGACAAATACAAGCTTGGATGCGTTCCTCTTTGATTCCACTAGAGTGTATCTCTTAGATTGGCAGAGGCGCATCAACATCATTGAAGGGATTGCACAAGGACTTCTTTATCTTCATAAGCACTCTCGAGTAAGAGTCATACACAGAGATCTGAAAGCTAGCAACATTTTACTCGACGAAAGCATGAAACCTAAGATATCAGACTTTGGAATGGCCAGAATCTTTGGACAGAATGAGTCCAGAGCAAATACAAACAGAGTTGTAGGAACATACGGCTATATGTCTCCAGAATATGCCATGAATGGCATTTTCTCCGAGAAGTCTGATGTATACAGCTTTGGAGTTTTACTGCTAGAGATCGTTAGTGGCAGAAAGAACACTGTTTTTGCTTGGGGCTTATGGAAACAAGGTGACTGTCTAGAGATAAGGGACACATCAATGGACTCGTGCCCAAATGACGAAGTTTCGAAATATGTTCATGTTGGTCTGCTATGTGCGCAAGAATACGTAGCAGATAGACCTACCATGTCAGAAGTCCTATCCATGCTCACAAGTGATATCATCTTTTTACCTGATCCAAAACAACCTGCATTCTGCGTCTCAAAAAGTGAATCCGGATCATCACAAGCTGAAAGGTCGGATATGGGTTCATCAAATGGTCTATCTATAACTGTCATGGAACCTAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

319

Amino Acids

35.63

Weight (kDa)

5.37

Isoelectric Point (pI)

44.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	15 - 272	1.8e-52	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	15 - 214	1.6e-45	Protein kinase domain
DUF3403	PF11883	278 - 318	7e-08	Domain of unknown function (DUF3403)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001889)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G18470
fragaria_vesca	FvH4_1g04840 FvH4_4g02170
malus_domestica	MD02G1047300.v1.1 MD02G1047500.v1.1 MD02G1047600.v1.1 MD15G1186100.v1.1
prunus_persica	Prupe.7G230600_v2.0.a1 Prupe.7G230700_v2.0.a1
pyrus_communis	pycom02g03950 pycom02g03960 pycom02g03970 pycom02g03980 pycom15g16680
rosa_chinensis	RchiOBHm_Chr2g0090491 RchiOBHm_Chr4g0389481 RchiOBHm_Chr4g0389511
rosa_laevigata	RLG00000009978 RLG00000009979 RLG00000016118
rosa_multiflora	Rmu_co8294841.1_g000001 Rmu_co8421003.1_g000001 Rmu_sc0002498.1_g000012 Rmu_sc0002498.1_g000014 Rmu_sc0004514.1_g000025 Rmu_sc0015016.1_g000001 Rmu_sc0016342.1_g000001 Rmu_sc0023528.1_g000001
rosa_roxburghii	Rroxscaffold_2G00151090 Rroxscaffold_5G00335480
rosa_rugosa	Rorug02G0008500 Rorug03G0323400 Rorug03G0323500 Rorug03G0323600
rosa_samantha	Rh2AG053900 Rh2BG053400 Rh2CG055500 Rh2DG054100 Rh4AG027900 Rh4AG028000 Rh4BG021100 Rh4CG029900 Rh4DG021500
rosa_wichuraiana	Rw2G004310 Rw4G002030 Rw4G002070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	765
Acc36I	ACCTGC	1 cut(s)	862
AccI	GTMKAC	2 cut(s)	84, 597
AccIII	TCCGGA	1 cut(s)	881
AclWI	GGATC	2 cut(s)	836, 892
AcoI	YGGCCR	1 cut(s)	480
AcsI	RAATTY	2 cut(s)	161, 249
AgeI	ACCGGT	1 cut(s)	79
AgsI	TTSAA	3 cut(s)	197, 236, 353
AleI	CACNNNNGTG	1 cut(s)	311
AluBI	AGCT	5 cut(s)	188, 282, 425, 603, 896
AluI	AGCT	5 cut(s)	188, 282, 425, 603, 896
Alw26I	GTCTC	3 cut(s)	121, 555, 871
AlwI	GGATC	2 cut(s)	836, 892
Ama87I	CYCGRG	1 cut(s)	393
Aor13HI	TCCGGA	1 cut(s)	881
AoxI	GGCC	1 cut(s)	480
ApoI	RAATTY	2 cut(s)	161, 249
ArsI	GACNNNNNNTTYG	2 cut(s)	29, 61
AsiGI	ACCGGT	1 cut(s)	79
AspLEI	GCGC	2 cut(s)	339, 766
AspS9I	GGNCC	1 cut(s)	77
AsuHPI	GGTGA	1 cut(s)	689
AsuII	TTCGAA	1 cut(s)	734
AsuNHI	GCTAGC	1 cut(s)	425
AvaI	CYCGRG	1 cut(s)	393
AvaII	GGWCC	1 cut(s)	77
BaeGI	GKGCMC	1 cut(s)	719
BalI	TGGCCA	1 cut(s)	482
BauI	CACGAG	1 cut(s)	712
BbsI	GAAGAC	1 cut(s)	61
BceAI	ACGGC	1 cut(s)	556
BcoDI	GTCTC	3 cut(s)	121, 555, 871
BfaI	CTAG	6 cut(s)	59, 309, 426, 620, 686, 951
BfuAI	ACCTGC	1 cut(s)	862
BglII	AGATCT	1 cut(s)	415
Bme18I	GGWCC	1 cut(s)	77
BmeT110I	CYCGRG	1 cut(s)	393
BmgT120I	GGNCC	1 cut(s)	77
BmiI	GGNNCC	1 cut(s)	948
BmsI	GCATC	2 cut(s)	277, 348
BmtI	GCTAGC	1 cut(s)	429
BpiI	GAAGAC	1 cut(s)	61
BplI	GAGNNNNNCTC	2 cut(s)	303, 335
BpmI	CTGGAG	1 cut(s)	537
Bpu14I	TTCGAA	1 cut(s)	734
BsaAI	YACGTR	1 cut(s)	775
BsaWI	WCCGGW	2 cut(s)	79, 881
Bse118I	RCCGGY	1 cut(s)	79
BseAI	TCCGGA	1 cut(s)	881
BseGI	GGATG	1 cut(s)	292
BseSI	GKGCMC	1 cut(s)	719
BshFI	GGCC	1 cut(s)	482
BshTI	ACCGGT	1 cut(s)	79
BsiHKCI	CYCGRG	1 cut(s)	393
BsiSI	CCGG	2 cut(s)	80, 882
BslFI	GGGAC	1 cut(s)	710
BsmAI	GTCTC	3 cut(s)	121, 555, 871
BsmBI	CGTCTC	1 cut(s)	871
BsmFI	GGGAC	1 cut(s)	710
BsmI	GAATGC	2 cut(s)	231, 857
BsnI	GGCC	1 cut(s)	482
BsoBI	CYCGRG	1 cut(s)	393
Bsp119I	TTCGAA	1 cut(s)	734
Bsp1286I	GDGCHC	1 cut(s)	719
Bsp13I	TCCGGA	1 cut(s)	881
Bsp143I	GATC	5 cut(s)	255, 415, 624, 841, 884
BspANI	GGCC	1 cut(s)	482
BspEI	TCCGGA	1 cut(s)	881
BspHI	TCATGA	1 cut(s)	165
BspLI	GGNNCC	1 cut(s)	948
BspMI	ACCTGC	1 cut(s)	862
BspOI	GCTAGC	1 cut(s)	429
BspPI	GGATC	2 cut(s)	836, 892
BspT104I	TTCGAA	1 cut(s)	734
BsrFI	RCCGGY	1 cut(s)	79
BssAI	RCCGGY	1 cut(s)	79
BssMI	GATC	5 cut(s)	255, 415, 624, 841, 884
BssNAI	GTATAC	1 cut(s)	598
BssSI	CACGAG	1 cut(s)	712
Bst1107I	GTATAC	1 cut(s)	598
Bst2BI	CACGAG	1 cut(s)	712
Bst4CI	ACNGT	3 cut(s)	649, 683, 940
Bst6I	CTCTTC	1 cut(s)	237
BstBAI	YACGTR	1 cut(s)	775
BstBI	TTCGAA	1 cut(s)	734
BstC8I	GCNNGC	1 cut(s)	427
BstDEI	CTNAG	2 cut(s)	322, 459
BstF5I	GGATG	1 cut(s)	292
BstHHI	GCGC	2 cut(s)	339, 766
BstKTI	GATC	5 cut(s)	258, 418, 627, 844, 887
BstMAI	GTCTC	3 cut(s)	121, 555, 871
BstMBI	GATC	5 cut(s)	255, 415, 624, 841, 884
BstMWI	GCNNNNNNNGC	1 cut(s)	763
BstSLI	GKGCMC	1 cut(s)	719
BstSNI	TACGTA	1 cut(s)	775
BstV2I	GAAGAC	1 cut(s)	61
BstX2I	RGATCY	1 cut(s)	415
BstYI	RGATCY	1 cut(s)	415
BstZ17I	GTATAC	1 cut(s)	598
BsuRI	GGCC	1 cut(s)	482
BtsCI	GGATG	1 cut(s)	292
BtsIMutI	CAGTG	1 cut(s)	645
BveI	ACCTGC	1 cut(s)	862
Cac8I	GCNNGC	1 cut(s)	427
CciI	TCATGA	1 cut(s)	165
CfoI	GCGC	2 cut(s)	339, 766
Cfr10I	RCCGGY	1 cut(s)	79
Cfr13I	GGNCC	1 cut(s)	77
CseI	GACGC	1 cut(s)	853
CspAI	ACCGGT	1 cut(s)	79
CviAII	CATG	7 cut(s)	166, 451, 565, 746, 793, 811, 943
CviJI	RGCY	9 cut(s)	188, 217, 282, 425, 482, 543, 603, 663, 896
CviKI_1	RGCY	9 cut(s)	188, 217, 282, 425, 482, 543, 603, 663, 896
DdeI	CTNAG	2 cut(s)	322, 459
DpnI	GATC	5 cut(s)	257, 417, 626, 843, 886
DpnII	GATC	5 cut(s)	255, 415, 624, 841, 884
EaeI	YGGCCR	1 cut(s)	480
Eam1104I	CTCTTC	1 cut(s)	237
EarI	CTCTTC	1 cut(s)	237
Eco105I	TACGTA	1 cut(s)	775
Eco32I	GATATC	2 cut(s)	465, 826
Eco47I	GGWCC	1 cut(s)	77
Eco88I	CYCGRG	1 cut(s)	393
EcoRI	GAATTC	1 cut(s)	161
EcoRV	GATATC	2 cut(s)	465, 826
Esp3I	CGTCTC	1 cut(s)	871
FaeI	CATG	7 cut(s)	169, 454, 568, 749, 796, 814, 946
FalI	AAGNNNNNCTT	2 cut(s)	357, 389
FaqI	GGGAC	1 cut(s)	710
FatI	CATG	7 cut(s)	165, 450, 564, 745, 792, 810, 942
FblI	GTMKAC	2 cut(s)	84, 597
FokI	GGATG	1 cut(s)	299
FspBI	CTAG	6 cut(s)	59, 309, 426, 620, 686, 951
FspI	TGCGCA	1 cut(s)	765
GlaI	GCGC	2 cut(s)	338, 765
GsuI	CTGGAG	1 cut(s)	537
HaeIII	GGCC	1 cut(s)	482
HapII	CCGG	2 cut(s)	80, 882
HgaI	GACGC	1 cut(s)	853
HhaI	GCGC	2 cut(s)	339, 766
Hin1II	CATG	7 cut(s)	169, 454, 568, 749, 796, 814, 946
Hin6I	GCGC	2 cut(s)	337, 764
HinP1I	GCGC	2 cut(s)	337, 764
HindIII	AAGCTT	1 cut(s)	280
HinfI	GANTC	6 cut(s)	302, 402, 486, 503, 710, 878
HpaII	CCGG	2 cut(s)	80, 882
HphI	GGTGA	1 cut(s)	689
Hpy166II	GTNNAC	2 cut(s)	85, 598
Hpy188I	TCNGA	7 cut(s)	255, 420, 469, 583, 592, 799, 907
Hpy188III	TCNNGA	8 cut(s)	14, 144, 166, 393, 507, 554, 686, 882
Hpy8I	GTNNAC	2 cut(s)	85, 598
Hpy99I	CGWCG	1 cut(s)	446
HpyAV	CCTTC	1 cut(s)	347
HpyCH4III	ACNGT	3 cut(s)	649, 683, 940
HpyCH4IV	ACGT	1 cut(s)	774
HpyCH4V	TGCA	5 cut(s)	29, 134, 231, 362, 857
HpyF10VI	GCNNNNNNNGC	1 cut(s)	763
HpyF3I	CTNAG	2 cut(s)	322, 459
HpySE526I	ACGT	1 cut(s)	774
Hsp92II	CATG	7 cut(s)	169, 454, 568, 749, 796, 814, 946
HspAI	GCGC	2 cut(s)	337, 764
Kpn2I	TCCGGA	1 cut(s)	881
Kzo9I	GATC	5 cut(s)	255, 415, 624, 841, 884
LweI	GCATC	2 cut(s)	277, 348
MaeI	CTAG	6 cut(s)	59, 309, 426, 620, 686, 951
MaeII	ACGT	1 cut(s)	774
MaeIII	GTNAC	2 cut(s)	22, 677
MalI	GATC	5 cut(s)	257, 417, 626, 843, 886
MboI	GATC	5 cut(s)	255, 415, 624, 841, 884
MboII	GAAGA	4 cut(s)	66, 170, 254, 371
MflI	RGATCY	1 cut(s)	415
MhlI	GDGCHC	1 cut(s)	719
MlsI	TGGCCA	1 cut(s)	482
MluCI	AATT	3 cut(s)	17, 161, 249
MluNI	TGGCCA	1 cut(s)	482
MlyI	GAGTC	3 cut(s)	411, 512, 704
MmeI	TCCRAC	1 cut(s)	885
MnlI	CCTC	3 cut(s)	58, 305, 327
Mox20I	TGGCCA	1 cut(s)	482
MroI	TCCGGA	1 cut(s)	881
MscI	TGGCCA	1 cut(s)	482
MslI	CAYNNNNRTG	2 cut(s)	311, 704
Msp20I	TGGCCA	1 cut(s)	482
MspI	CCGG	2 cut(s)	80, 882
Mva1269I	GAATGC	2 cut(s)	231, 857
MwoI	GCNNNNNNNGC	1 cut(s)	763
NdeII	GATC	5 cut(s)	255, 415, 624, 841, 884
NheI	GCTAGC	1 cut(s)	425
NlaIII	CATG	7 cut(s)	169, 454, 568, 749, 796, 814, 946
NlaIV	GGNNCC	1 cut(s)	948
NmuCI	GTSAC	2 cut(s)	22, 677
NsbI	TGCGCA	1 cut(s)	765
NspV	TTCGAA	1 cut(s)	734
OliI	CACNNNNGTG	1 cut(s)	311
PaeR7I	CTCGAG	1 cut(s)	393
PagI	TCATGA	1 cut(s)	165
PctI	GAATGC	2 cut(s)	231, 857
PfeI	GAWTC	3 cut(s)	302, 486, 878
PinAI	ACCGGT	1 cut(s)	79
PleI	GAGTC	3 cut(s)	410, 511, 704
PpsI	GAGTC	3 cut(s)	410, 511, 704
Ppu21I	YACGTR	1 cut(s)	775
PspN4I	GGNNCC	1 cut(s)	948
PspPI	GGNCC	1 cut(s)	77
PsuI	RGATCY	1 cut(s)	415
RseI	CAYNNNNRTG	2 cut(s)	311, 704
Sau3AI	GATC	5 cut(s)	255, 415, 624, 841, 884
Sau96I	GGNCC	1 cut(s)	77
SchI	GAGTC	3 cut(s)	411, 512, 704
SduI	GDGCHC	1 cut(s)	719
SfaNI	GCATC	2 cut(s)	277, 348
Sfr274I	CTCGAG	1 cut(s)	393
SfuI	TTCGAA	1 cut(s)	734
SinI	GGWCC	1 cut(s)	77
SlaI	CTCGAG	1 cut(s)	393
SmiMI	CAYNNNNRTG	2 cut(s)	311, 704
SmlI	CTYRAG	1 cut(s)	393
SmoI	CTYRAG	1 cut(s)	393
SnaBI	TACGTA	1 cut(s)	775
Sse9I	AATT	3 cut(s)	17, 161, 249
SspMI	CTAG	6 cut(s)	59, 309, 426, 620, 686, 951
TaaI	ACNGT	3 cut(s)	649, 683, 940
TaiI	ACGT	1 cut(s)	777
TaqI	TCGA	3 cut(s)	394, 441, 734
TasI	AATT	3 cut(s)	17, 161, 249
TfiI	GAWTC	3 cut(s)	302, 486, 878
TscAI	CASTG	1 cut(s)	652
TseFI	GTSAC	2 cut(s)	22, 677
Tsp45I	GTSAC	2 cut(s)	22, 677
TspDTI	ATGAA	8 cut(s)	154, 182, 276, 371, 467, 581, 734, 906
TspRI	CASTG	1 cut(s)	652
VpaK11BI	GGWCC	1 cut(s)	77
XapI	RAATTY	2 cut(s)	161, 249
XbaI	TCTAGA	1 cut(s)	685
XhoI	CTCGAG	1 cut(s)	393
XmiI	GTMKAC	2 cut(s)	84, 597
XspI	CTAG	6 cut(s)	59, 309, 426, 620, 686, 951

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.