MD15G1186100.v1.1

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001889
Physical Location & Seq
Reverse (-)
14695136 .. 14701893
6758 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1186100.v1.1.491

Sequence Viewer

Length: 2964 bp
ATGTGCTGCGTGCAATCTGTTTTTTTCCTCTTGTCATGCACAAACCCTAGTTATCTTGTACTACTCAAGCAAGGTCAGCAGCTAGTAGATCGAAATCAGGATCGTCTTGCTTCGGAAAATGGCAGTTTCTTTAAGTTAAGCTTCTTGAGTCCTGGTACTTCAAGCGCTTCCGCGATACCAGCGATCGTTACGTGGGGCACAAAGCTTCCAATAAGTCATCCGGATGCAGTGTGGGTGGCTAATTCGAAAACTCCGCTCAGTGATTCATCAGGTGTTTTTACTTCGGATAGTGATGCAAAATTGAAGATTGTGTATGGTGGAGGGCAGATACCCGGTGTGTTAGCAGATGGCCAACAGATAGCAGTTAAGAGATTTGCAAGAAATTCGGGACAGGGACTAGAAGAATTCATAAACGAGATTACACTTATAGCTGAACTTCAACACAGCAATCTTGTTCAGATTCTTGGTTGTTGCATTCAAGGAGAAGAGAAGATTCTGCTCTATGAGTTCCTGACGAATACAAGCTTCGATGGCCTTCCTTTTCGGTTTGTCCACAACTCCTTTCGAATCCAAAGACACACTTTAGTGGTGTACACGCTCAAGCAAGGCGACCAGCTACGGGACCAGGACACCGATCATCTCGTTTCTCCGCACGGTTACTTCAAGCTGGGGTTCTTCAGTCCCGGTGCTTCCAGTGACCTGGGTGCTACGAGCAATCGGTTCTTGGGAATATGGTACAGCAAACTACCAAATCATCCGGATGCGGTGTGGGTGGCAAACCCTGCAACTCCGGTCGTGGATTCGTCTGGAGTGTTTATGTTGGATAGTGATGGGAAAATGAAGATCAAACATGGTGGAGGGGAGATTATTCTGTGGGATCACAATCAGACAGTTTCCGGTAATGTGACTGGCTATATGATGGATAGCGGAAATTTTATGTTAAGGGAAGTAGCATCGGATGGAACTCCCGGAAAGATTTTGTGGGAAAGTTTTGATTACCCATCAAACACATTGTTACCTGGAATGAAGTTAGGCTGGGACTTTAGGACCGGGCGTAATCGAACAGCTTCTTCATGGTTCAGTGGTCAGGTGCCTACGCCAGGTGCTTTCCGGCTTGGTGTGGATCCCAATCGCACCAACCGGTTAATCATCTGGCGGCGAGACGTTGTATACTGGACCAGTGGGATCTGGGAAAGTGGAAGTTTTCAGATGGCCCCTGAACTGACAAGCAGGGCTGATTTGTTCGAGTTCTCTTTTGTTTCAAATGCAGAGGAGAAGTACTTCACTTATTCTGTCAAAGACAACTCTACTCTTTCGAGATGGGAACTCAATACTTGGGGCCAACTCTTGCAGTCGATTTTAGCCTCAGACGGTACAACATGGGAAACCAAAGCTTCAGGTCCATGTACGTTCAACCCAGATTATCCGGATGCAGTGTGCATAAAGCAGACGACCTCTGAATGCAGGAATGGTTCCGAGCTATTCCTACCGATAAGAGGCTACTTAATTGATGCTCAGTTGACATATAATGATAGTAACCCTAACTCGGATCTTAGCGACTGTCACGCAATTTGCTGGAGGGATTGCGCCTGTATCGGCTACGGACCTTTACAGCTCAATGGGTCTGGATGTCTGTATATGAGAGAAGGAGCACATTTTGTCAAGAATAATTACTTTGGAGTTAATTACCTCGTTACCAAAGCAAATAATAGCAGAGATGGAACACCTACCGAAGGAAGTAAAAAGAGCAAATGGTGGATATGGTGTATCGTAGGGATTATACTTGCAGTGGCAGTGCTACTTTTGGGGTACTTCCTCTATACAAGGAAAAGAAAACCTCAACACGTGCAACAAACTGACAATTCGGAAACAATCCAAGAGCAAGGGTTACTTGACTTGGGATCTCAAATCAGCGGAATGAGTGACATATTCAAACTCAAATTTGGCAAAAGCCGGCGGCAGGAATTTAAGATGTTCAGTTTTTCTGAATTAGTGGCTGCAACAGATGACTTCTCATTTGCCCGAAGGCTAGGAGAGGGTGGTTTTGGACCGGTTTACAAGGGTGTATTACCAGACGGGCAACAGGTAGCAGTAAAGAGACTTGCAAGACATTCAGGACAAGGACTAGAAGAATTCATGAATGAGATAACACTTATAGCTGAACTTCAACATAGTAATCTTGTTCGGCTTTTGGGTTGTTGCATCCAAGGAGAAGAGAAGATTCTGATCTATGAATACTTGACAAATACAAGCTTGGATGCCTTCCTCTTTGATTCTACTAGAGTGTATCTCTTAGATTGGCAGAGGCGCATCAACATCATTGAAGGGATTGCACAAGGACTTCTTTATCTTCATAAGTACTCTAGAGTTAGAATCATACACAGAGATTTGAAAGCTAGCAACATTTTACTTGACGAAAGCATGAAACCTAAGATATCAGATTTTGGAATGGCCAGAATCTTCGGACAGGATGAGTCCAGAGCAAATACGAAGAGAGTTGTAGGAACATATGGCTATATGTCTCCAGAGTATGCCATGAATGACATTTTCTCTGAGAAGTCTGATGTATACAGCTTTGGAGTTCTACTGCTGGAGATCGTGAGTGGCAGAAAGAACACTGTATTCGTTTCATCTACTAATCTCAGCCTTATTGAACTTGCCTGGGGTTTATGGCAACAAGGTGACTGTCTAGAGATAAGGGACCCATCAATGGACTCATGTCCAAAAGACGAAGGTTCGAAATATGTTCATGTTGGTCTGCTATGTGTGCAGGAACACGCAGCAGATAGACCTACTATGTCGGAAGTAATATCCATGCTCACAAGTGATGTCATGTTTTTACCAGATCCAAAACAGCCCGCATTCTATGTCTCAAAAAGTGAATCCGGATCATCCCAAGCTGAAATAAGGTCGGATAGGGGTTCTTCAAATTGTGTATCTATAACTGTAATGGAACCTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

988

Amino Acids

110.18

Weight (kDa)

5.91

Isoelectric Point (pI)

38.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 117 - 176 3.2e-07 Protein tyrosine and serine/threonine kinase
B_lectin PF01453 256 - 359 4.9e-25 D-mannose binding lectin
S_locus_glycop PF00954 392 - 488 1.9e-08 S-locus glycoprotein domain
PK_Tyr_Ser-Thr PF07714 673 - 940 2e-50 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 673 - 872 2.3e-44 Protein kinase domain
DUF3403 PF11883 945 - 987 9.9e-06 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1090
AccBSI CCGCTC 1 cut(s) 256
AccI GTMKAC 2 cut(s) 1170, 2568
AccII CGCG 1 cut(s) 173
AccIII TCCGGA 4 cut(s) 220, 757, 1426, 2885
AciI CCGC 9 cut(s) 171, 254, 650, 764, 927, 1156, 1914, 1957, 2859
AclWI GGATC 9 cut(s) 108, 885, 1118, 1131, 1193, 1557, 1909, 2840, 2896
AcoI YGGCCR 2 cut(s) 349, 2451
AcsI RAATTY 6 cut(s) 382, 404, 931, 1940, 1964, 2132
AcuI CTGAAG 2 cut(s) 661, 1380
AcvI CACGTG 1 cut(s) 1846
AfaI GTAC 9 cut(s) 60, 157, 593, 737, 1280, 1375, 1408, 1811, 2360
AfeI AGCGCT 1 cut(s) 166
AfiI CCNNNNNNNGG 7 cut(s) 619, 1100, 1496, 1546, 1733, 1960, 2710
AflIII ACRYGT 1 cut(s) 1843
AgeI ACCGGT 2 cut(s) 1140, 2050
AjnI CCWGG 6 cut(s) 151, 624, 699, 1018, 1099, 2660
AleI CACNNNNGTG 1 cut(s) 584
Alw21I GWGCWC 1 cut(s) 1654
Alw26I GTCTC 4 cut(s) 1155, 2092, 2526, 2875
AlwI GGATC 9 cut(s) 108, 885, 1118, 1131, 1193, 1557, 1909, 2840, 2896
Aor13HI TCCGGA 4 cut(s) 220, 757, 1426, 2885
Aor51HI AGCGCT 1 cut(s) 166
AoxI GGCC 5 cut(s) 349, 532, 1212, 1339, 2451
ApeKI GCWGC 4 cut(s) 6, 79, 1997, 2780
ApoI RAATTY 6 cut(s) 382, 404, 931, 1940, 1964, 2132
ArsI GACNNNNNNTTYG 2 cut(s) 2000, 2032
AsiGI ACCGGT 2 cut(s) 1140, 2050
Asp700I GAANNNNTTC 2 cut(s) 1066, 1280
AspLEI GCGC 3 cut(s) 167, 1589, 2310
AspS9I GGNCC 9 cut(s) 622, 1047, 1176, 1213, 1339, 1400, 1604, 2048, 2701
AsuC2I CCSGG 4 cut(s) 333, 684, 969, 1051
AsuHPI GGTGA 1 cut(s) 2693
AsuII TTCGAA 3 cut(s) 245, 565, 2738
AsuNHI GCTAGC 1 cut(s) 2396
AvaII GGWCC 7 cut(s) 622, 1047, 1176, 1400, 1604, 2048, 2701
BaeGI GKGCMC 1 cut(s) 200
BalI TGGCCA 2 cut(s) 351, 2453
BamHI GGATCC 1 cut(s) 1123
BanI GGYRCC 1 cut(s) 1090
BbrPI CACGTG 1 cut(s) 1846
Bbv12I GWGCWC 1 cut(s) 1654
BbvI GCAGC 3 cut(s) 91, 1984, 2792
BcgI CGANNNNNNTGC 2 cut(s) 366, 400
BciT130I CCWGG 6 cut(s) 153, 626, 701, 1020, 1101, 2662
BcnI CCSGG 4 cut(s) 333, 684, 969, 1051
BcoDI GTCTC 4 cut(s) 1155, 2092, 2526, 2875
BfoI RGCGCY 1 cut(s) 168
BisI GCNGC 6 cut(s) 7, 80, 1157, 1958, 1998, 2781
BlsI GCNGC 6 cut(s) 8, 81, 1158, 1959, 1999, 2782
BmcAI AGTACT 2 cut(s) 1280, 2360
Bme18I GGWCC 7 cut(s) 622, 1047, 1176, 1400, 1604, 2048, 2701
BmgT120I GGNCC 9 cut(s) 622, 1047, 1176, 1213, 1339, 1400, 1604, 2048, 2701
BmiI GGNNCC 9 cut(s) 623, 1092, 1125, 1215, 1340, 1474, 2702, 2703, 2955
BmsI GCATC 9 cut(s) 214, 283, 751, 962, 1420, 1501, 2211, 2248, 2319
BmtI GCTAGC 1 cut(s) 2400
BoxI GACNNNNGTC 1 cut(s) 2718
BplI GAGNNNNNCTC 2 cut(s) 2274, 2306
BpmI CTGGAG 4 cut(s) 828, 1597, 2508, 2612
Bpu14I TTCGAA 3 cut(s) 245, 565, 2738
BpuEI CTTGAG 3 cut(s) 50, 166, 584
BpuMI CCSGG 4 cut(s) 333, 684, 969, 1051
BsaAI YACGTR 2 cut(s) 192, 1846
BsaBI GATNNNNATC 4 cut(s) 93, 882, 1128, 2225
BsaJI CCNNGG 3 cut(s) 700, 2206, 2661
BsaWI WCCGGW 8 cut(s) 220, 757, 790, 896, 1140, 1426, 2050, 2885
Bsc4I CCNNNNNNNGG 7 cut(s) 619, 1100, 1496, 1546, 1733, 1960, 2710
Bse118I RCCGGY 3 cut(s) 1140, 1953, 2050
Bse1I ACTGG 4 cut(s) 693, 913, 1178, 1179
Bse8I GATNNNNATC 4 cut(s) 93, 882, 1128, 2225
BseAI TCCGGA 4 cut(s) 220, 757, 1426, 2885
BseBI CCWGG 6 cut(s) 153, 626, 701, 1020, 1101, 2662
BseDI CCNNGG 3 cut(s) 700, 2206, 2661
BseJI GATNNNNATC 4 cut(s) 93, 882, 1128, 2225
BseLI CCNNNNNNNGG 7 cut(s) 619, 1100, 1496, 1546, 1733, 1960, 2710
BseMII CTCAG 5 cut(s) 271, 1380, 1529, 2544, 2656
BseNI ACTGG 4 cut(s) 693, 913, 1178, 1179
BseRI GAGGAG 1 cut(s) 1286
BseSI GKGCMC 1 cut(s) 200
BseXI GCAGC 3 cut(s) 91, 1984, 2792
BseYI CCCAGC 2 cut(s) 667, 1035
BsgI GTGCAG 1 cut(s) 2789
Bsh1236I CGCG 1 cut(s) 173
Bsh1285I CGRYCG 2 cut(s) 186, 795
BshFI GGCC 5 cut(s) 351, 534, 1214, 1341, 2453
BshNI GGYRCC 1 cut(s) 1090
BshTI ACCGGT 2 cut(s) 1140, 2050
BsiEI CGRYCG 2 cut(s) 186, 795
BsiHKAI GWGCWC 1 cut(s) 1654
BslFI GGGAC 6 cut(s) 402, 408, 635, 666, 1052, 2714
BslI CCNNNNNNNGG 7 cut(s) 619, 1100, 1496, 1546, 1733, 1960, 2710
BsmAI GTCTC 4 cut(s) 1155, 2092, 2526, 2875
BsmBI CGTCTC 1 cut(s) 1155
BsmFI GGGAC 6 cut(s) 402, 408, 635, 666, 1052, 2714
BsmI GAATGC 3 cut(s) 474, 1466, 2861
BsnI GGCC 5 cut(s) 351, 534, 1214, 1341, 2453
Bsp119I TTCGAA 3 cut(s) 245, 565, 2738
Bsp1286I GDGCHC 2 cut(s) 200, 1654
Bsp13I TCCGGA 4 cut(s) 220, 757, 1426, 2885
Bsp1407I TGTACA 1 cut(s) 591
BspACI CCGC 9 cut(s) 171, 254, 650, 764, 927, 1156, 1914, 1957, 2859
BspANI GGCC 5 cut(s) 351, 534, 1214, 1341, 2453
BspCNI CTCAG 5 cut(s) 270, 1379, 1528, 2545, 2655
BspEI TCCGGA 4 cut(s) 220, 757, 1426, 2885
BspFNI CGCG 1 cut(s) 173
BspHI TCATGA 1 cut(s) 2136
BspLI GGNNCC 9 cut(s) 623, 1092, 1125, 1215, 1340, 1474, 2702, 2703, 2955
BspOI GCTAGC 1 cut(s) 2400
BspPI GGATC 9 cut(s) 108, 885, 1118, 1131, 1193, 1557, 1909, 2840, 2896
BspT104I TTCGAA 3 cut(s) 245, 565, 2738
BspT107I GGYRCC 1 cut(s) 1090
BsrBI CCGCTC 1 cut(s) 256
BsrFI RCCGGY 3 cut(s) 1140, 1953, 2050
BsrGI TGTACA 1 cut(s) 591
BsrI ACTGG 4 cut(s) 693, 913, 1178, 1179
BssAI RCCGGY 3 cut(s) 1140, 1953, 2050
BssECI CCNNGG 3 cut(s) 700, 2206, 2661
BssNAI GTATAC 2 cut(s) 1171, 2569
BssT1I CCWWGG 1 cut(s) 2206
Bst1107I GTATAC 2 cut(s) 1171, 2569
Bst2UI CCWGG 6 cut(s) 153, 626, 701, 1020, 1101, 2662
Bst4CI ACNGT 7 cut(s) 656, 892, 1373, 1562, 2620, 2687, 2947
Bst6I CTCTTC 3 cut(s) 480, 2208, 2486
BstAPI GCANNNNNTGC 1 cut(s) 782
BstAUI TGTACA 1 cut(s) 591
BstBAI YACGTR 2 cut(s) 192, 1846
BstBI TTCGAA 3 cut(s) 245, 565, 2738
BstC8I GCNNGC 4 cut(s) 11, 1955, 2398, 2859
BstDEI CTNAG 8 cut(s) 257, 1366, 1515, 1553, 2293, 2430, 2553, 2642
BstENI CCTNNNNNAGG 2 cut(s) 1098, 1731
BstFNI CGCG 1 cut(s) 173
BstH2I RGCGCY 1 cut(s) 168
BstHHI GCGC 3 cut(s) 167, 1589, 2310
BstMAI GTCTC 4 cut(s) 1155, 2092, 2526, 2875
BstMCI CGRYCG 2 cut(s) 186, 795
BstMWI GCNNNNNNNGC 5 cut(s) 76, 179, 531, 782, 2767
BstNI CCWGG 6 cut(s) 153, 626, 701, 1020, 1101, 2662
BstPAI GACNNNNGTC 1 cut(s) 2718
BstSLI GKGCMC 1 cut(s) 200
BstUI CGCG 1 cut(s) 173
BstV1I GCAGC 3 cut(s) 91, 1984, 2792
BstX2I RGATCY 5 cut(s) 1123, 1185, 1549, 1901, 2845
BstXI CCANNNNNNTGG 1 cut(s) 700
BstYI RGATCY 5 cut(s) 1123, 1185, 1549, 1901, 2845
BstZ17I GTATAC 2 cut(s) 1171, 2569
BsuRI GGCC 5 cut(s) 351, 534, 1214, 1341, 2453
BtsI GCAGTG 4 cut(s) 234, 1440, 1794, 1800
BtsIMutI CAGTG 9 cut(s) 234, 265, 700, 1087, 1186, 1440, 1794, 1800, 2616
Cac8I GCNNGC 4 cut(s) 11, 1955, 2398, 2859
CciI TCATGA 1 cut(s) 2136
CfoI GCGC 3 cut(s) 167, 1589, 2310
Cfr10I RCCGGY 3 cut(s) 1140, 1953, 2050
Cfr13I GGNCC 9 cut(s) 622, 1047, 1176, 1213, 1339, 1400, 1604, 2048, 2701
Csp6I GTAC 9 cut(s) 59, 156, 592, 736, 1279, 1374, 1407, 1810, 2359
CspAI ACCGGT 2 cut(s) 1140, 2050
CspCI CAANNNNNGTGG 2 cut(s) 835, 870
CviQI GTAC 9 cut(s) 59, 156, 592, 736, 1279, 1374, 1407, 1810, 2359
DdeI CTNAG 8 cut(s) 257, 1366, 1515, 1553, 2293, 2430, 2553, 2642
EaeI YGGCCR 2 cut(s) 349, 2451
Eam1104I CTCTTC 3 cut(s) 480, 2208, 2486
EarI CTCTTC 3 cut(s) 480, 2208, 2486
Eco130I CCWWGG 1 cut(s) 2206
Eco32I GATATC 1 cut(s) 2436
Eco47I GGWCC 7 cut(s) 622, 1047, 1176, 1400, 1604, 2048, 2701
Eco47III AGCGCT 1 cut(s) 166
Eco57I CTGAAG 2 cut(s) 661, 1380
Eco72I CACGTG 1 cut(s) 1846
EcoNI CCTNNNNNAGG 2 cut(s) 1098, 1731
EcoO109I RGGNCCY 1 cut(s) 2701
EcoRI GAATTC 2 cut(s) 404, 2132
EcoRII CCWGG 6 cut(s) 151, 624, 699, 1018, 1099, 2660
EcoRV GATATC 1 cut(s) 2436
EcoT14I CCWWGG 1 cut(s) 2206
ErhI CCWWGG 1 cut(s) 2206
Esp3I CGTCTC 1 cut(s) 1155
FalI AAGNNNNNCTT 8 cut(s) 125, 157, 565, 597, 1875, 1907, 2328, 2360
FaqI GGGAC 6 cut(s) 402, 408, 635, 666, 1052, 2714
FauI CCCGC 1 cut(s) 2866
FauNDI CATATG 1 cut(s) 2509
FblI GTMKAC 2 cut(s) 1170, 2568
Fnu4HI GCNGC 6 cut(s) 7, 80, 1157, 1958, 1998, 2781
Fsp4HI GCNGC 6 cut(s) 7, 80, 1157, 1958, 1998, 2781
GlaI GCGC 3 cut(s) 166, 1588, 2309
GluI GCNGC 6 cut(s) 7, 80, 1157, 1958, 1998, 2781
GsaI CCCAGC 2 cut(s) 671, 1039
GsuI CTGGAG 4 cut(s) 828, 1597, 2508, 2612
HaeII RGCGCY 1 cut(s) 168
HaeIII GGCC 5 cut(s) 351, 534, 1214, 1341, 2453
HhaI GCGC 3 cut(s) 167, 1589, 2310
Hin6I GCGC 3 cut(s) 165, 1587, 2308
HinP1I GCGC 3 cut(s) 165, 1587, 2308
HincII GTYRAC 1 cut(s) 1521
HindII GTYRAC 1 cut(s) 1521
HindIII AAGCTT 5 cut(s) 139, 203, 523, 1392, 2251
HphI GGTGA 1 cut(s) 2693
Hpy166II GTNNAC 7 cut(s) 553, 592, 594, 1171, 1521, 2056, 2569
Hpy8I GTNNAC 7 cut(s) 553, 592, 594, 1171, 1521, 2056, 2569
HpyAV CCTTC 7 cut(s) 545, 1640, 1727, 2019, 2272, 2318, 2726
HpyCH4III ACNGT 7 cut(s) 656, 892, 1373, 1562, 2620, 2687, 2947
HpyCH4IV ACGT 4 cut(s) 191, 1164, 1409, 1845
HpyF10VI GCNNNNNNNGC 5 cut(s) 76, 179, 531, 782, 2767
HpyF3I CTNAG 8 cut(s) 257, 1366, 1515, 1553, 2293, 2430, 2553, 2642
HpySE526I ACGT 4 cut(s) 191, 1164, 1409, 1845
HspAI GCGC 3 cut(s) 165, 1587, 2308
KflI GGGWCCC 1 cut(s) 2701
Kpn2I TCCGGA 4 cut(s) 220, 757, 1426, 2885
KroI GCCGGC 1 cut(s) 1953
KroNI GCCGGC 1 cut(s) 1955
LmnI GCTCC 1 cut(s) 1649
Lsp1109I GCAGC 3 cut(s) 91, 1984, 2792
LweI GCATC 9 cut(s) 214, 283, 751, 962, 1420, 1501, 2211, 2248, 2319
MaeII ACGT 4 cut(s) 191, 1164, 1409, 1845
MbiI CCGCTC 1 cut(s) 256
MflI RGATCY 5 cut(s) 1123, 1185, 1549, 1901, 2845
MhlI GDGCHC 2 cut(s) 200, 1654
MlsI TGGCCA 2 cut(s) 351, 2453
MluNI TGGCCA 2 cut(s) 351, 2453
MlyI GAGTC 3 cut(s) 157, 2483, 2708
MmeI TCCRAC 3 cut(s) 801, 2781, 2892
Mox20I TGGCCA 2 cut(s) 351, 2453
MroI TCCGGA 4 cut(s) 220, 757, 1426, 2885
MroNI GCCGGC 1 cut(s) 1953
MroXI GAANNNNTTC 2 cut(s) 1066, 1280
MscI TGGCCA 2 cut(s) 351, 2453
MseI TTAA 8 cut(s) 132, 137, 366, 941, 1145, 1505, 1683, 1968
MslI CAYNNNNRTG 3 cut(s) 222, 584, 759
Msp20I TGGCCA 2 cut(s) 351, 2453
MspA1I CMGCKG 1 cut(s) 1914
Mva1269I GAATGC 3 cut(s) 474, 1466, 2861
MvaI CCWGG 6 cut(s) 153, 626, 701, 1020, 1101, 2662
MvnI CGCG 1 cut(s) 173
MwoI GCNNNNNNNGC 5 cut(s) 76, 179, 531, 782, 2767
NaeI GCCGGC 1 cut(s) 1955
NciI CCSGG 4 cut(s) 333, 684, 969, 1051
NdeI CATATG 1 cut(s) 2509
NgoMIV GCCGGC 1 cut(s) 1953
NheI GCTAGC 1 cut(s) 2396
NlaIV GGNNCC 9 cut(s) 623, 1092, 1125, 1215, 1340, 1474, 2702, 2703, 2955
NmuCI GTSAC 5 cut(s) 695, 904, 1562, 1922, 2681
NspV TTCGAA 3 cut(s) 245, 565, 2738
OliI CACNNNNGTG 1 cut(s) 584
PagI TCATGA 1 cut(s) 2136
PctI GAATGC 3 cut(s) 474, 1466, 2861
PdiI GCCGGC 1 cut(s) 1955
PdmI GAANNNNTTC 2 cut(s) 1066, 1280
PfoI TCCNGGA 1 cut(s) 967
PinAI ACCGGT 2 cut(s) 1140, 2050
PkrI GCNGC 6 cut(s) 8, 81, 1158, 1959, 1999, 2782
Ple19I CGATCG 1 cut(s) 186
PleI GAGTC 3 cut(s) 156, 2482, 2708
PmaCI CACGTG 1 cut(s) 1846
PmlI CACGTG 1 cut(s) 1846
PpsI GAGTC 3 cut(s) 156, 2482, 2708
Ppu21I YACGTR 2 cut(s) 192, 1846
PpuMI RGGWCCY 1 cut(s) 2701
PshAI GACNNNNGTC 1 cut(s) 2718
Psp5II RGGWCCY 1 cut(s) 2701
Psp6I CCWGG 6 cut(s) 151, 624, 699, 1018, 1099, 2660
PspCI CACGTG 1 cut(s) 1846
PspFI CCCAGC 2 cut(s) 667, 1035
PspGI CCWGG 6 cut(s) 151, 624, 699, 1018, 1099, 2660
PspN4I GGNNCC 9 cut(s) 623, 1092, 1125, 1215, 1340, 1474, 2702, 2703, 2955
PspPI GGNCC 9 cut(s) 622, 1047, 1176, 1213, 1339, 1400, 1604, 2048, 2701
PspPPI RGGWCCY 1 cut(s) 2701
PsuI RGATCY 5 cut(s) 1123, 1185, 1549, 1901, 2845
PvuI CGATCG 1 cut(s) 186
RsaI GTAC 9 cut(s) 60, 157, 593, 737, 1280, 1375, 1408, 1811, 2360
RsaNI GTAC 9 cut(s) 59, 156, 592, 736, 1279, 1374, 1407, 1810, 2359
RseI CAYNNNNRTG 3 cut(s) 222, 584, 759
SaqAI TTAA 8 cut(s) 132, 137, 366, 941, 1145, 1505, 1683, 1968
SatI GCNGC 6 cut(s) 7, 80, 1157, 1958, 1998, 2781
Sau96I GGNCC 9 cut(s) 622, 1047, 1176, 1213, 1339, 1400, 1604, 2048, 2701
ScaI AGTACT 2 cut(s) 1280, 2360
SchI GAGTC 3 cut(s) 157, 2483, 2708
SduI GDGCHC 2 cut(s) 200, 1654
SfaNI GCATC 9 cut(s) 214, 283, 751, 962, 1420, 1501, 2211, 2248, 2319
SfuI TTCGAA 3 cut(s) 245, 565, 2738
SinI GGWCC 7 cut(s) 622, 1047, 1176, 1400, 1604, 2048, 2701
SmiMI CAYNNNNRTG 3 cut(s) 222, 584, 759
SmlI CTYRAG 3 cut(s) 65, 145, 599
SmoI CTYRAG 3 cut(s) 65, 145, 599
SsiI CCGC 9 cut(s) 171, 254, 650, 764, 927, 1156, 1914, 1957, 2859
StyI CCWWGG 1 cut(s) 2206
TaaI ACNGT 7 cut(s) 656, 892, 1373, 1562, 2620, 2687, 2947
TaiI ACGT 4 cut(s) 194, 1167, 1412, 1848
TaqI TCGA 9 cut(s) 91, 245, 528, 565, 1060, 1245, 1316, 1355, 2738
TatI WGTACW 4 cut(s) 58, 591, 1278, 2358
TauI GCSGC 2 cut(s) 1159, 1960
Tru1I TTAA 8 cut(s) 132, 137, 366, 941, 1145, 1505, 1683, 1968
Tru9I TTAA 8 cut(s) 132, 137, 366, 941, 1145, 1505, 1683, 1968
TscAI CASTG 9 cut(s) 234, 265, 700, 1087, 1186, 1440, 1794, 1800, 2623
TseFI GTSAC 5 cut(s) 695, 904, 1562, 1922, 2681
TseI GCWGC 4 cut(s) 6, 79, 1997, 2780
Tsp45I GTSAC 5 cut(s) 695, 904, 1562, 1922, 2681
TspGWI ACGGA 1 cut(s) 1617
TspRI CASTG 9 cut(s) 234, 265, 700, 1087, 1186, 1440, 1794, 1800, 2623
VpaK11BI GGWCC 7 cut(s) 622, 1047, 1176, 1400, 1604, 2048, 2701
XagI CCTNNNNNAGG 2 cut(s) 1098, 1731
XapI RAATTY 6 cut(s) 382, 404, 931, 1940, 1964, 2132
XbaI TCTAGA 2 cut(s) 2363, 2689
XmiI GTMKAC 2 cut(s) 1170, 2568
XmnI GAANNNNTTC 2 cut(s) 1066, 1280
ZrmI AGTACT 2 cut(s) 1280, 2360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.