RchiOBHm_Chr4g0389511

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0001889
Physical Location & Seq
Reverse (-)
4446161 .. 4449386
3226 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36264

Sequence Viewer

Length: 2307 bp
ATGGCCAAACTAAGAAACACTGTCTTCCTCTATTTCTCATGTTTCTGCATGCATTATTTTTCTTCTTCTCATGCACAAACACAAAATGCAACACTCAAGCAAGGCCAACAGCTACGCGATCAGGATGGTGATTATCTTGTTTCCGAAAATGGTTTGTTTAAGTTAGGCTTCTTCAGTCCTGGAAACTCAAGCGTTTTCGGTGCTACAAGTAATCGTTACTTAGGAATATGGTACTACAAACTACCAAATGATCCAGATGCAGTATGGGTAGCCAATCCAGAAACTCCGATCATTGATACATCAGGAGTATTTGCATTGGCTAGTGATGCAACATTGAAGCTCATTCATGGTGGAGGCCAAATATCTGTCTCAGATCCCAATCAAACAGTTTCTGGTAATGTGACTGTCTCCTTATTAGATACTGGAAATTTGGTCTTAAGAGAAGTAACTTCTAATGGAACACATGGAGAAGTTCTGTGGGAAAGCTTTGACCATCCAACCAACACATTGTTACCTGGAATGAAATTGGGTATCAATGTCGAGGCTGGACAGAATTGGACTATTTCTTCCTGGTTCAGCGATCAAATCCCTGCAACAGGTGTTTTTAGGCTTGGCGTTGATCCTGGTAGCACCAACCAATTGATCATCTGGAGGCGAGATGAAGTATACTGGAGCAGTGGGGTCTGGGAAAATGGAACCTTTCAAAATGCTCCTGAAATGACAAGAAGGGTTGATCTATTTGAGTTCACTTTTGTTTCAAACAAGGAAGATAAGTACTTTACTTATACCGTTAAAAACAGGTCTACTGTTTCAAGGTGGGAACTGAATGCTTGGGGCCAAATCATGCACTCCACTCTCAACGGTACTACTTGGGAAAGCACAGAAGCTAGTCCATGCACATTCAACTTGACTAATCTGGGAGCCCTGTGCATAGAGGAGAAGCCCTCTGAATGCAGGAATGGCTCGGACGGACTTGTACCAGTAAGAGGCTATTTTGATGGAAACGAAATTTTACATCATGACAACAATACTGAAATGACCATTAGTGATTGCCATGCTACTTGCTGGAGTGACTGCGCCTGTATTGGCTACAAAAGTATATATACCAATGGGACTGGGTGTGTGTTTATGAGTGGAGAAGCGAAATTTATCCCGAATAGTTACTTTGATGCTACTTATGTCCTTAACATATGGAATCATAGCAAAGGCCAAGACCAAGGATTAGATGAGTTGAGATCTCAAATTACCAGATTTGGCCACATATACAAGCTCAAAATAGGTGAAAGAATAGGACAGAACTTTCGGATGTTCAGTTTTTCAGAAGTAGTGGCTGCAACAGATAACTTCTCGTCTGCCAGCAAGCTAGGACAGGGTGGGTTTGGACCTGTTTACAAGGGGGTATTACCAGATGGGGAACAGGTAGGAGTAAAGAGACTCGCGAGAAGTTCAGGACAAGGACTAGAAGAATTCATGAATGAGATTACACTTATAGCTGAGCTTCAACATAGTAATCTTGTTAGGCTATTAGGTTGTTGCATTCAAGGAGAAGAAAAGATTCTGATTTACGAATTTATGGCAAATAAGAGCTTGGATGCCTTCCTATTTGACTCCACTAGAAGGAAGAAATTGGACTGGCAAACACTGGTAAACATCATTGAGGGAATTGCACAAGGACTTCTCTATCTTCATAAGCATTCTAGAGTTAGAGTCATACACAGAGATCTGAAAGCTAGTAACATTCTGCTTGATGAAAATATGAACCCTAAGATCTCAGACTTTGGAATGGCCAGAATTTTCGGACAGAATGAATCTATGGCAAATACAGTTAGAGTTGTCGGAACATACGGCTATATGTCTCCAGAGTATGCCATGAGGGGCATTTTCTCTGAAAAGTCTGATGTTTACAGTTTTGGTGTTCTACTGTTGGAGATTATAAGTGGCAAAAAGAACACTACTTTTCTTTCATCTAATGGTCTCTGCCTTATCGAACTTGCCTGGGAATTGTGGAAACAAGGCCAGACCCTGGAGATAAAGGACACATCACTTGACTGTGGTTCCAAAGAGGAAGTTTTAAAATTTATTCAGGTTGGTCTCTTATGTGTTCAAGAGTATGCAGAAGATAGAGCTACTATGTCAGAAGTAATATCAATGCTTACAAGTGATGTCACACCTTTGTCTGATCCAAAGCAACCTGCATACACTATTTCAAGAAGTGAAGCAGCTGGGTCAACGCGCACGTTACCTGATAGAAGTTCAAATACAGCTTCAGTAAATAATGTATCCATTACTTTGATGGAAGGTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

768

Amino Acids

85.53

Weight (kDa)

5.36

Isoelectric Point (pI)

38.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 88 - 191 2.7e-27 D-mannose binding lectin
S_locus_glycop PF00954 224 - 299 2.8e-08 S-locus glycoprotein domain
Pkinase PF00069 450 - 715 3.6e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 452 - 717 7.2e-50 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1934
Acc36I ACCTGC 1 cut(s) 2200
AccB7I CCANNNNNTGG 1 cut(s) 2023
AccI GTMKAC 2 cut(s) 666, 803
AccII CGCG 3 cut(s) 117, 1439, 2233
AclWI GGATC 4 cut(s) 245, 368, 614, 2174
AcoI YGGCCR 3 cut(s) 3, 1255, 1785
AcsI RAATTY 7 cut(s) 427, 1008, 1145, 1466, 1568, 1791, 2075
AcuI CTGAAG 2 cut(s) 157, 2250
AfaI GTAC 4 cut(s) 233, 776, 865, 978
AfiI CCNNNNNNNGG 4 cut(s) 596, 986, 1617, 2023
AflII CTTAAG 1 cut(s) 436
AjnI CCWGG 6 cut(s) 178, 514, 569, 622, 1994, 2022
Alw26I GTCTC 6 cut(s) 373, 412, 1426, 1860, 1979, 2096
AlwI GGATC 4 cut(s) 245, 368, 614, 2174
AlwNI CAGNNNCTG 2 cut(s) 392, 2023
AoxI GGCC 8 cut(s) 3, 103, 355, 835, 1207, 1255, 1785, 2014
ApeKI GCWGC 2 cut(s) 1331, 2219
ApoI RAATTY 7 cut(s) 427, 1008, 1145, 1466, 1568, 1791, 2075
ArsI GACNNNNNNTTYG 2 cut(s) 1284, 1316
Asp700I GAANNNNTTC 1 cut(s) 1554
AspLEI GCGC 2 cut(s) 1079, 2235
AspS9I GGNCC 2 cut(s) 835, 1382
AsuHPI GGTGA 2 cut(s) 140, 1292
AvaII GGWCC 1 cut(s) 1382
BalI TGGCCA 3 cut(s) 5, 1257, 1787
BanII GRGCYC 1 cut(s) 925
BarI GAAGNNNNNNTAC 2 cut(s) 2242, 2274
BbsI GAAGAC 1 cut(s) 16
BbvI GCAGC 2 cut(s) 1318, 2231
BccI CCATC 5 cut(s) 119, 501, 992, 1403, 2287
BceAI ACGGC 1 cut(s) 1861
BciT130I CCWGG 6 cut(s) 180, 516, 571, 624, 1996, 2024
BciVI GTATCC 1 cut(s) 2290
BclI TGATCA 1 cut(s) 642
BcoDI GTCTC 6 cut(s) 373, 412, 1426, 1860, 1979, 2096
BfaI CTAG 7 cut(s) 321, 888, 1364, 1460, 1614, 1698, 1731
BfrI CTTAAG 1 cut(s) 436
BfuAI ACCTGC 1 cut(s) 2200
BfuI GTATCC 1 cut(s) 2290
BglII AGATCT 3 cut(s) 1235, 1720, 1767
BisI GCNGC 2 cut(s) 1332, 2220
BlpI GCTNAGC 1 cut(s) 1494
BlsI GCNGC 2 cut(s) 1333, 2221
BmcAI AGTACT 1 cut(s) 776
Bme1390I CCNGG 6 cut(s) 180, 516, 571, 624, 1996, 2024
Bme18I GGWCC 1 cut(s) 1382
BmgT120I GGNCC 2 cut(s) 835, 1382
BmiI GGNNCC 4 cut(s) 697, 836, 922, 2056
BmrFI CCNGG 6 cut(s) 180, 516, 571, 624, 1996, 2024
BmrI ACTGGG 1 cut(s) 1125
BmsI GCATC 4 cut(s) 247, 316, 1159, 1582
BmuI ACTGGG 1 cut(s) 1125
BpiI GAAGAC 1 cut(s) 16
BplI GAGNNNNNCTC 2 cut(s) 929, 961
BpmI CTGGAG 5 cut(s) 670, 691, 1087, 1842, 2045
Bpu1102I GCTNAGC 1 cut(s) 1494
BpuEI CTTGAG 2 cut(s) 80, 172
BsaBI GATNNNNATC 1 cut(s) 378
BsaI GGTCTC 2 cut(s) 1979, 2096
BsaJI CCNNGG 3 cut(s) 1216, 1995, 2022
Bsc4I CCNNNNNNNGG 4 cut(s) 596, 986, 1617, 2023
Bse1I ACTGG 6 cut(s) 427, 674, 980, 1120, 1637, 1647
Bse8I GATNNNNATC 1 cut(s) 378
BseBI CCWGG 6 cut(s) 180, 516, 571, 624, 1996, 2024
BseDI CCNNGG 3 cut(s) 1216, 1995, 2022
BseGI GGATG 4 cut(s) 130, 493, 1311, 1597
BseJI GATNNNNATC 1 cut(s) 378
BseLI CCNNNNNNNGG 4 cut(s) 596, 986, 1617, 2023
BseMII CTCAG 3 cut(s) 384, 1485, 1785
BseNI ACTGG 6 cut(s) 427, 674, 980, 1120, 1637, 1647
BseRI GAGGAG 1 cut(s) 950
BseXI GCAGC 2 cut(s) 1318, 2231
BseYI CCCAGC 1 cut(s) 2222
Bsh1236I CGCG 3 cut(s) 117, 1439, 2233
BshFI GGCC 8 cut(s) 5, 105, 357, 837, 1209, 1257, 1787, 2016
BslFI GGGAC 1 cut(s) 1126
BslI CCNNNNNNNGG 4 cut(s) 596, 986, 1617, 2023
BsmAI GTCTC 6 cut(s) 373, 412, 1426, 1860, 1979, 2096
BsmFI GGGAC 1 cut(s) 1126
BsmI GAATGC 4 cut(s) 832, 956, 1536, 1693
BsnI GGCC 8 cut(s) 5, 105, 357, 837, 1209, 1257, 1787, 2016
Bso31I GGTCTC 2 cut(s) 1979, 2096
Bsp1286I GDGCHC 1 cut(s) 925
Bsp1720I GCTNAGC 1 cut(s) 1494
Bsp68I TCGCGA 1 cut(s) 1439
BspANI GGCC 8 cut(s) 5, 105, 357, 837, 1209, 1257, 1787, 2016
BspCNI CTCAG 3 cut(s) 383, 1486, 1784
BspFNI CGCG 3 cut(s) 117, 1439, 2233
BspHI TCATGA 2 cut(s) 1018, 1470
BspLI GGNNCC 4 cut(s) 697, 836, 922, 2056
BspMI ACCTGC 1 cut(s) 2200
BspPI GGATC 4 cut(s) 245, 368, 614, 2174
BspTI CTTAAG 1 cut(s) 436
BspTNI GGTCTC 2 cut(s) 1979, 2096
BsrI ACTGG 6 cut(s) 427, 674, 980, 1120, 1637, 1647
BssECI CCNNGG 3 cut(s) 1216, 1995, 2022
BssNAI GTATAC 1 cut(s) 667
BssT1I CCWWGG 1 cut(s) 1216
Bst1107I GTATAC 1 cut(s) 667
Bst2UI CCWGG 6 cut(s) 180, 516, 571, 624, 1996, 2024
BstAFI CTTAAG 1 cut(s) 436
BstC8I GCNNGC 3 cut(s) 50, 1357, 1361
BstDEI CTNAG 6 cut(s) 11, 220, 370, 1494, 1764, 1771
BstENI CCTNNNNNAGG 1 cut(s) 594
BstF5I GGATG 4 cut(s) 130, 493, 1311, 1597
BstFNI CGCG 3 cut(s) 117, 1439, 2233
BstHHI GCGC 2 cut(s) 1079, 2235
BstMAI GTCTC 6 cut(s) 373, 412, 1426, 1860, 1979, 2096
BstMWI GCNNNNNNNGC 2 cut(s) 326, 960
BstNI CCWGG 6 cut(s) 180, 516, 571, 624, 1996, 2024
BstNSI RCATGY 1 cut(s) 52
BstSCI CCNGG 6 cut(s) 178, 514, 569, 622, 1994, 2022
BstUI CGCG 3 cut(s) 117, 1439, 2233
BstV1I GCAGC 2 cut(s) 1318, 2231
BstV2I GAAGAC 1 cut(s) 16
BstX2I RGATCY 4 cut(s) 373, 1235, 1720, 1767
BstYI RGATCY 4 cut(s) 373, 1235, 1720, 1767
BstZ17I GTATAC 1 cut(s) 667
BsuI GTATCC 1 cut(s) 2290
BsuRI GGCC 8 cut(s) 5, 105, 357, 837, 1209, 1257, 1787, 2016
BtsCI GGATG 4 cut(s) 130, 493, 1311, 1597
BtsI GCAGTG 1 cut(s) 682
BtsIMutI CAGTG 3 cut(s) 18, 682, 1640
BtuMI TCGCGA 1 cut(s) 1439
BveI ACCTGC 1 cut(s) 2200
Cac8I GCNNGC 3 cut(s) 50, 1357, 1361
CaiI CAGNNNCTG 2 cut(s) 392, 2023
CciI TCATGA 2 cut(s) 1018, 1470
CfoI GCGC 2 cut(s) 1079, 2235
Cfr13I GGNCC 2 cut(s) 835, 1382
Csp6I GTAC 4 cut(s) 232, 775, 864, 977
CviQI GTAC 4 cut(s) 232, 775, 864, 977
DdeI CTNAG 6 cut(s) 11, 220, 370, 1494, 1764, 1771
DraI TTTAAA 1 cut(s) 2073
EaeI YGGCCR 3 cut(s) 3, 1255, 1785
Eco130I CCWWGG 1 cut(s) 1216
Eco24I GRGCYC 1 cut(s) 925
Eco31I GGTCTC 2 cut(s) 1979, 2096
Eco47I GGWCC 1 cut(s) 1382
Eco57I CTGAAG 2 cut(s) 157, 2250
EcoNI CCTNNNNNAGG 1 cut(s) 594
EcoRI GAATTC 1 cut(s) 1466
EcoRII CCWGG 6 cut(s) 178, 514, 569, 622, 1994, 2022
EcoT14I CCWWGG 1 cut(s) 1216
EcoT22I ATGCAT 1 cut(s) 54
EcoT38I GRGCYC 1 cut(s) 925
ErhI CCWWGG 1 cut(s) 1216
FalI AAGNNNNNCTT 2 cut(s) 152, 184
FaqI GGGAC 1 cut(s) 1126
FauNDI CATATG 1 cut(s) 1190
FbaI TGATCA 1 cut(s) 642
FblI GTMKAC 2 cut(s) 666, 803
Fnu4HI GCNGC 2 cut(s) 1332, 2220
FokI GGATG 4 cut(s) 137, 480, 1318, 1604
FriOI GRGCYC 1 cut(s) 925
Fsp4HI GCNGC 2 cut(s) 1332, 2220
FspBI CTAG 7 cut(s) 321, 888, 1364, 1460, 1614, 1698, 1731
GlaI GCGC 2 cut(s) 1078, 2234
GluI GCNGC 2 cut(s) 1332, 2220
GsaI CCCAGC 1 cut(s) 2226
GsuI CTGGAG 5 cut(s) 670, 691, 1087, 1842, 2045
HaeIII GGCC 8 cut(s) 5, 105, 357, 837, 1209, 1257, 1787, 2016
HhaI GCGC 2 cut(s) 1079, 2235
Hin6I GCGC 2 cut(s) 1077, 2233
HinP1I GCGC 2 cut(s) 1077, 2233
HincII GTYRAC 1 cut(s) 2229
HindII GTYRAC 1 cut(s) 2229
HindIII AAGCTT 1 cut(s) 484
HinfI GANTC 6 cut(s) 1195, 1434, 1555, 1607, 1707, 1808
HphI GGTGA 2 cut(s) 140, 1292
Hpy166II GTNNAC 7 cut(s) 667, 747, 804, 1390, 1648, 1903, 2229
Hpy8I GTNNAC 7 cut(s) 667, 747, 804, 1390, 1648, 1903, 2229
HpyAV CCTTC 4 cut(s) 720, 1606, 1611, 2291
HpyCH4IV ACGT 1 cut(s) 2237
HpyF10VI GCNNNNNNNGC 2 cut(s) 326, 960
HpyF3I CTNAG 6 cut(s) 11, 220, 370, 1494, 1764, 1771
HpySE526I ACGT 1 cut(s) 2237
HspAI GCGC 2 cut(s) 1077, 2233
Ksp22I TGATCA 1 cut(s) 642
LmnI GCTCC 3 cut(s) 672, 715, 920
Lsp1109I GCAGC 2 cut(s) 1318, 2231
LweI GCATC 4 cut(s) 247, 316, 1159, 1582
MaeI CTAG 7 cut(s) 321, 888, 1364, 1460, 1614, 1698, 1731
MaeII ACGT 1 cut(s) 2237
MaeIII GTNAC 9 cut(s) 215, 400, 445, 510, 1070, 1160, 1733, 2164, 2238
MfeI CAATTG 1 cut(s) 638
MflI RGATCY 4 cut(s) 373, 1235, 1720, 1767
MhlI GDGCHC 1 cut(s) 925
MlsI TGGCCA 3 cut(s) 5, 1257, 1787
MluNI TGGCCA 3 cut(s) 5, 1257, 1787
MlyI GAGTC 3 cut(s) 1428, 1601, 1716
MmeI TCCRAC 3 cut(s) 521, 1816, 1905
Mox20I TGGCCA 3 cut(s) 5, 1257, 1787
Mph1103I ATGCAT 1 cut(s) 54
MroXI GAANNNNTTC 1 cut(s) 1554
MscI TGGCCA 3 cut(s) 5, 1257, 1787
MseI TTAA 5 cut(s) 159, 437, 792, 1185, 2072
Msp20I TGGCCA 3 cut(s) 5, 1257, 1787
MspA1I CMGCKG 1 cut(s) 2222
MspCI CTTAAG 1 cut(s) 436
MspR9I CCNGG 6 cut(s) 180, 516, 571, 624, 1996, 2024
MunI CAATTG 1 cut(s) 638
Mva1269I GAATGC 4 cut(s) 832, 956, 1536, 1693
MvaI CCWGG 6 cut(s) 180, 516, 571, 624, 1996, 2024
MvnI CGCG 3 cut(s) 117, 1439, 2233
MwoI GCNNNNNNNGC 2 cut(s) 326, 960
NdeI CATATG 1 cut(s) 1190
NlaIV GGNNCC 4 cut(s) 697, 836, 922, 2056
NmuCI GTSAC 3 cut(s) 400, 1070, 2164
NruI TCGCGA 1 cut(s) 1439
NsiI ATGCAT 1 cut(s) 54
NspI RCATGY 1 cut(s) 52
PaeI GCATGC 1 cut(s) 52
PagI TCATGA 2 cut(s) 1018, 1470
PctI GAATGC 4 cut(s) 832, 956, 1536, 1693
PdmI GAANNNNTTC 1 cut(s) 1554
PfeI GAWTC 3 cut(s) 1195, 1555, 1808
PflMI CCANNNNNTGG 1 cut(s) 2023
PfoI TCCNGGA 1 cut(s) 178
PkrI GCNGC 2 cut(s) 1333, 2221
PleI GAGTC 3 cut(s) 1428, 1601, 1715
PpsI GAGTC 3 cut(s) 1428, 1601, 1715
PsiI TTATAA 1 cut(s) 1934
Psp6I CCWGG 6 cut(s) 178, 514, 569, 622, 1994, 2022
PspFI CCCAGC 1 cut(s) 2222
PspGI CCWGG 6 cut(s) 178, 514, 569, 622, 1994, 2022
PspN4I GGNNCC 4 cut(s) 697, 836, 922, 2056
PspPI GGNCC 2 cut(s) 835, 1382
PstNI CAGNNNCTG 2 cut(s) 392, 2023
PsuI RGATCY 4 cut(s) 373, 1235, 1720, 1767
PvuII CAGCTG 1 cut(s) 2222
RruI TCGCGA 1 cut(s) 1439
RsaI GTAC 4 cut(s) 233, 776, 865, 978
RsaNI GTAC 4 cut(s) 232, 775, 864, 977
SaqAI TTAA 5 cut(s) 159, 437, 792, 1185, 2072
SatI GCNGC 2 cut(s) 1332, 2220
Sau96I GGNCC 2 cut(s) 835, 1382
ScaI AGTACT 1 cut(s) 776
SchI GAGTC 3 cut(s) 1428, 1601, 1716
ScrFI CCNGG 6 cut(s) 180, 516, 571, 624, 1996, 2024
SduI GDGCHC 1 cut(s) 925
SfaNI GCATC 4 cut(s) 247, 316, 1159, 1582
SinI GGWCC 1 cut(s) 1382
SmlI CTYRAG 3 cut(s) 95, 187, 436
SmoI CTYRAG 3 cut(s) 95, 187, 436
SphI GCATGC 1 cut(s) 52
SspMI CTAG 7 cut(s) 321, 888, 1364, 1460, 1614, 1698, 1731
StyD4I CCNGG 6 cut(s) 178, 514, 569, 622, 1994, 2022
StyI CCWWGG 1 cut(s) 1216
TaiI ACGT 1 cut(s) 2240
TaqI TCGA 2 cut(s) 540, 1986
TatI WGTACW 1 cut(s) 774
TfiI GAWTC 3 cut(s) 1195, 1555, 1808
Tru1I TTAA 5 cut(s) 159, 437, 792, 1185, 2072
Tru9I TTAA 5 cut(s) 159, 437, 792, 1185, 2072
TscAI CASTG 3 cut(s) 25, 682, 1647
TseFI GTSAC 3 cut(s) 400, 1070, 2164
TseI GCWGC 2 cut(s) 1331, 2219
Tsp45I GTSAC 3 cut(s) 400, 1070, 2164
TspGWI ACGGA 1 cut(s) 984
TspRI CASTG 3 cut(s) 25, 682, 1647
Van91I CCANNNNNTGG 1 cut(s) 2023
Vha464I CTTAAG 1 cut(s) 436
VpaK11BI GGWCC 1 cut(s) 1382
XagI CCTNNNNNAGG 1 cut(s) 594
XapI RAATTY 7 cut(s) 427, 1008, 1145, 1466, 1568, 1791, 2075
XbaI TCTAGA 1 cut(s) 1697
XceI RCATGY 1 cut(s) 52
XcmI CCANNNNNNNNNTGG 2 cut(s) 261, 2290
XmiI GTMKAC 2 cut(s) 666, 803
XmnI GAANNNNTTC 1 cut(s) 1554
XspI CTAG 7 cut(s) 321, 888, 1364, 1460, 1614, 1698, 1731
ZrmI AGTACT 1 cut(s) 776
Zsp2I ATGCAT 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.