Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0003055

Physical Location & Seq

Genomic Coordinates Reverse (-)

14090075 .. 14096127

Gene Structure 6053 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g10410.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2466 bp

ATGGAGTTGAGAAGGCTTTCCATCTCCAAGCCTTGCTCCTTCTTAATCAAACGCCCGTTAAGCTGCGTCCCGCGTCCTCAGCCTCCGCCGCCGCCCCCGCCTCCGAACAACCATCGATTGCTGGACCGGGTATCCTCTATTCTCTCAAACTCATCTTTCAAGTATCCTCAATGTAGATCTCTCATCTCCCTCTTGTCCCCTCATGATTTCGATACCATGTTCTACTCCCTCGCCACTTCCCCCTCCTCCAATCTCAACCCCAAAACTGCTCTCCGCTTCTTCCATTTCGCCTCCAGAGCTTTTAACTTCCATTTTACGCTTCGCTCTTATTGCCTTTTGGTTCGTTTACTTCTTCTCTCCAATCTTAAACTCCCCGCTCGATTGCTTCTGATTCGCTTGATCGATGGCAATGTGCGTGCTCTCTATGCTCTCCCGCCCACGAACAGGCATGTTGAGATAGCCATGGCTATGTCCGAGTTCTCAACTGCTTCTGAAAGAGCTGCTGTTCTTCAGGCATTGGATATCTTGGTTCATCTATACTGTACCCAGTTCAAGGATTCTGGTTTTTGTTCTGCTATTGATGTTTTTGAGGTTTTCTCTAATAAGGCTATGTTTCCGTCCCTCAAGACTTGCCATTTTTTGTTGAATTCTTTAGTCAAGGCTGACCAACTTGATAAGAGCTACCGTTTATTTGAACTTCTGTCTCGTGGTGTTTCTCTGGATGTTTACTTGTTTACTACTGCCATTCAGGCATTCTGTAAGGGAGGCAAGGTTGATGATGCAATTGGTTTGTTCTCTAGAATGGAGGCACTAGGCATTGCTCCAAATGTTGTTACTTACAACAATGTTATTCATGGGCTGTGTAAGAGCAGACGATTAGAGGAGGCTTTCGGATTCAAGGACAAGATGGTAGAAAACAATGTGAATCCCAGTCGTACGACCTATGGTGCGCTCCTTAATGGTTTGATTAAGCTGGACAAGTTTGAAGATGCTAATTGTGTTTTGAAGGAAATGTATAGTAAGGGATTCGTCCCCAACCAGGTTATCTATAACATACTGATTGACGGCTATTGTAAAACGGGAAGTCTTAGTGTGGCACTGAACTTATGGGATGATATGTTATCCAATGGGGCAACTCCAAATTCTGTTACCATTAACTCTCTACTGCAGGGACTTTGTAAAAGCAATCACTTTGAGCATGCTGAGCAGGTCTTAGATAAGATGCTATCCAGTTGTTCCTCTGTAGACCACGTTGTTTGTTTCTCAGTCATTCACTCGTTATGCAAGAACTGCAGGTTTGATTCTGCACTGAAATTCACAATTGAAATGCTTTCAAGAAACTTCAGGCCCAGTGATAACTTGCTTACGACATTGGTTGTTGGGCTCTGTAAAGATGGAAAGCATTCTGAGGCAACTCGTCTGTGGTTTAGGCTATGGGAGAAAGGGTTTGCAGCCAACACAATGACTTCAAATGCTCTGATTCATGGAGTTTGTGAATCCTGTAACATGCAAGAGGTTGCTAAGCTACTCAAGGAAATGGTAGAGAGAGGCTTGGTACTGGATAGGATCTCATGCAATACACTCATCTTCGGTTGGTGCAAGGAGGGAAAAGTGGAGGAAGCTTTCAAGCTTAGGGACTGGATGGGTAAGAAAGGAATTTTGCCAGACACATATACTTACAATTTGCTAATGCATGGTCTATGTCGTTTGGGGAAAGTGGATGATGCTGTTAAACTTTTGGAGGAGTGCAGAAAACATGGTCTTGCCGATGTCTATACATATGGGGTGATGATAGATGGGTACTATAAAGCTAACAGAACCAAAGAGGGTGAACACCTATTTATTGAGTTGGTGACTAAGAAACTACAGCTAAATTTCATTGTTTATAATACACTTATCAGAGCATACTGTACAAATGCGAACATGATAGGAGCCCTTGGTCTCTGCTGTGACATGACAAAGAAGGGTATTCAACCAACTTGTACCACATATTCTTCTCTTATACATGGGATGTGCAATATTGGCAATGTTGAAGATGCAAAAGACCTTCTTGATCAAATTAGGATGGAAGGTTTGTTGCCTGATGTTGTTTGCTATACTGCACTAATTCATGGTTATTGTAAGCTAGGCCAAATGGATAAAGTAGGGAGTGTTCTTGGAGAGATGTCTTCATATAACATACAACCTAATGTGATTACGTACACTGTCATGATTGATGGGCATTGTAAACTAGGTAATATGGAAGAAGCAACTAAACTTATATGCGAGATGAAAAGAAAGGGAATTGTCCCGGATGCTGTCACTTACAATGCCTTAACAAATGGATTTTGCAAGGACAGGAAGGTGGAAGAAGCTTTTGAAGTTTGTGATCAAATGTTCAGTAAGGGTCTAACTTTAGATGAAATTACCTATACGACATTGGTTAGTGGCCTGCATCAAGCTACTACATGTGCAAACGAGGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

822

Amino Acids

92.02

Weight (kDa)

8.28

Isoelectric Point (pI)

31.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_long	PF17177	181 - 320	3.8e-08	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	234 - 284	1.8e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	236 - 269	1.3e-08	PPR repeat
PPR_2	PF13041	241 - 289	5.1e-14	PPR repeat family
PPR_1	PF12854	272 - 304	7.1e-11	PPR repeat
PPR_2	PF13041	275 - 324	2.1e-15	PPR repeat family
PPR	PF01535	278 - 308	1.4e-06	PPR repeat
PPR_3	PF13812	300 - 357	3.6e-06	Pentatricopeptide repeat domain
TPR_24	PF23276	334 - 438	3.3e-07	Fungal tetratrico peptide repeats
PPR_1	PF12854	341 - 373	4.8e-10	PPR repeat
PPR_2	PF13041	345 - 394	5.5e-17	PPR repeat family
PPR	PF01535	348 - 377	5.5e-07	PPR repeat
PPR_1	PF12854	377 - 408	9.7e-07	PPR repeat
PPR_2	PF13041	380 - 410	3.4e-06	PPR repeat family
PPR_3	PF13812	508 - 565	4.9e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	516 - 548	2.4e-07	PPR repeat
PPR_2	PF13041	522 - 569	6e-18	PPR repeat family
PPR_1	PF12854	551 - 582	4.8e-12	PPR repeat
PPR_2	PF13041	555 - 603	6e-13	PPR repeat family
PPR	PF01535	558 - 588	3.9e-07	PPR repeat
PPR_3	PF13812	613 - 669	8e-08	Pentatricopeptide repeat domain
PPR_long	PF17177	614 - 729	5e-07	Pentacotripeptide-repeat region of PRORP
TPR_24	PF23276	619 - 714	1.3e-07	Fungal tetratrico peptide repeats
PPR_2	PF13041	627 - 673	7.4e-13	PPR repeat family
PPR_1	PF12854	655 - 687	1.4e-09	PPR repeat
PPR_3	PF13812	683 - 738	1.2e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	691 - 714	6.5e-08	PPR repeat
PPR_2	PF13041	694 - 743	5.1e-18	PPR repeat family
PPR	PF01535	697 - 726	1.9e-06	PPR repeat
PPR_3	PF13812	717 - 774	6.1e-11	Pentatricopeptide repeat domain
PPR_long	PF17177	718 - 808	5.8e-10	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	726 - 758	6.5e-11	PPR repeat
PPR	PF01535	732 - 762	3.7e-10	PPR repeat
PPR_2	PF13041	734 - 778	4.6e-16	PPR repeat family
PPR_1	PF12854	760 - 792	8.5e-12	PPR repeat
PPR_2	PF13041	764 - 812	2.5e-14	PPR repeat family
PPR	PF01535	767 - 797	5.4e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003055)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G19440 AT4G19440 AT4G19440 AT4G19440
fragaria_vesca	FvH4_4g10410 FvH4_4g10410 FvH4_4g10440 FvH4_5g24430 FvH4_5g24430 FvH4_5g24430 FvH4_5g24430
malus_domestica	MD06G1022200.v1.1
prunus_persica	Prupe.3G018200_v2.0.a1
pyrus_communis	pycom06g01610
rosa_chinensis	RchiOBHm_Chr4g0405931 RchiOBHm_Chr7g0214821 RchiOBHm_Chr7g0219861
rosa_laevigata	RLG00000002716 RLG00000008810
rosa_multiflora	Rmu_sc0002308.1_g000021 Rmu_sc0019702.1_g000004 Rmu_ssc0000087.1_g000071
rosa_roxburghii	Rroxscaffold_3G00244980 Rroxscaffold_5G00350670
rosa_rugosa	Rorug04G0064100 Rorug07G0147600 Rorug07G0147700 Rorug07G0147800
rosa_samantha	Rh4AG134900 Rh4BG130500 Rh4CG142300 Rh4DG129200 Rh7AG284500 Rh7BG275300 Rh7CG303700 Rh7DG287900
rosa_wichuraiana	Rw4G010980 Rw7G024260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1887
Acc36I	ACCTGC	2 cut(s)	1198, 1284
AccBSI	CCGCTC	1 cut(s)	377
AccI	GTMKAC	1 cut(s)	1245
AccII	CGCG	1 cut(s)	73
AciI	CCGC	8 cut(s)	71, 86, 89, 92, 98, 274, 375, 434
AclWI	GGATC	1 cut(s)	1574
AcsI	RAATTY	5 cut(s)	646, 1141, 1313, 1656, 1873
AcuI	CTGAAG	2 cut(s)	494, 1327
AfaI	GTAC	7 cut(s)	544, 937, 1557, 1802, 1912, 1984, 2201
AfiI	CCNNNNNNNGG	3 cut(s)	444, 553, 1039
AflIII	ACRYGT	1 cut(s)	2447
AjnI	CCWGG	1 cut(s)	1038
Alw21I	GWGCWC	1 cut(s)	421
Alw26I	GTCTC	2 cut(s)	708, 1946
AlwI	GGATC	1 cut(s)	1574
AoxI	GGCC	3 cut(s)	1346, 2128, 2428
ApeKI	GCWGC	3 cut(s)	63, 500, 1451
ApoI	RAATTY	5 cut(s)	646, 1141, 1313, 1656, 1873
Asp700I	GAANNNNTTC	2 cut(s)	16, 1402
AspLEI	GCGC	1 cut(s)	952
AspS9I	GGNCC	2 cut(s)	124, 1347
AsuC2I	CCSGG	2 cut(s)	128, 2291
AsuHPI	GGTGA	3 cut(s)	1798, 1841, 1864
AvaII	GGWCC	1 cut(s)	124
BanII	GRGCYC	2 cut(s)	1386, 1936
BauI	CACGAG	1 cut(s)	705
BbsI	GAAGAC	1 cut(s)	2160
Bbv12I	GWGCWC	1 cut(s)	421
BbvCI	CCTCAGC	1 cut(s)	78
BbvI	GCAGC	3 cut(s)	50, 487, 1463
BccI	CCATC	9 cut(s)	29, 120, 398, 901, 1388, 1636, 1790, 2059, 2210
BceAI	ACGGC	1 cut(s)	1081
BciT130I	CCWGG	1 cut(s)	1040
BciVI	GTATCC	2 cut(s)	142, 174
BclI	TGATCA	2 cut(s)	2053, 2368
BcnI	CCSGG	2 cut(s)	128, 2291
BcoDI	GTCTC	2 cut(s)	708, 1946
BfaI	CTAG	4 cut(s)	798, 812, 2126, 2231
BfmI	CTRYAG	4 cut(s)	1166, 1242, 1291, 1865
BfuAI	ACCTGC	2 cut(s)	1198, 1284
BfuI	GTATCC	2 cut(s)	142, 174
BglI	GCCNNNNNGGC	1 cut(s)	749
BglII	AGATCT	1 cut(s)	176
BisI	GCNGC	5 cut(s)	64, 89, 92, 501, 1452
BlpI	GCTNAGC	2 cut(s)	1203, 1521
BlsI	GCNGC	5 cut(s)	65, 90, 93, 502, 1453
Bme1390I	CCNGG	3 cut(s)	128, 1040, 2291
Bme18I	GGWCC	1 cut(s)	124
BmgT120I	GGNCC	2 cut(s)	124, 1347
BmiI	GGNNCC	1 cut(s)	1933
BmrFI	CCNGG	3 cut(s)	128, 1040, 2291
BmrI	ACTGGG	3 cut(s)	541, 924, 1344
BmsI	GCATC	7 cut(s)	769, 979, 1212, 1714, 2026, 2284, 2443
BmuI	ACTGGG	3 cut(s)	541, 924, 1344
BpiI	GAAGAC	1 cut(s)	2160
BplI	GAGNNNNNCTC	2 cut(s)	581, 613
BpmI	CTGGAG	1 cut(s)	277
Bpu10I	CCTNAGC	2 cut(s)	78, 1631
Bpu1102I	GCTNAGC	2 cut(s)	1203, 1521
BpuEI	CTTGAG	2 cut(s)	608, 1514
BpuMI	CCSGG	2 cut(s)	128, 2291
Bsa29I	ATCGAT	2 cut(s)	115, 402
BsaAI	YACGTR	1 cut(s)	2199
BsaI	GGTCTC	1 cut(s)	1946
BsaJI	CCNNGG	2 cut(s)	462, 1936
BsaXI	ACNNNNNCTCC	4 cut(s)	756, 786, 1430, 1460
Bsc4I	CCNNNNNNNGG	3 cut(s)	444, 553, 1039
Bse1I	ACTGG	6 cut(s)	547, 930, 1230, 1350, 1563, 1643
Bse3DI	GCAATG	3 cut(s)	415, 816, 2032
BseBI	CCWGG	1 cut(s)	1040
BseCI	ATCGAT	2 cut(s)	115, 402
BseDI	CCNNGG	2 cut(s)	462, 1936
BseGI	GGATG	7 cut(s)	727, 1117, 1647, 1726, 2016, 2070, 2299
BseLI	CCNNNNNNNGG	3 cut(s)	444, 553, 1039
BseMI	GCAATG	3 cut(s)	415, 816, 2032
BseMII	CTCAG	4 cut(s)	92, 1194, 1278, 1398
BseNI	ACTGG	6 cut(s)	547, 930, 1230, 1350, 1563, 1643
BseRI	GAGGAG	3 cut(s)	235, 896, 1757
BseXI	GCAGC	3 cut(s)	50, 487, 1463
BsgI	GTGCAG	3 cut(s)	1290, 1768, 2085
Bsh1236I	CGCG	1 cut(s)	73
BshFI	GGCC	3 cut(s)	1348, 2130, 2430
BshVI	ATCGAT	2 cut(s)	115, 402
BsiHKAI	GWGCWC	1 cut(s)	421
BsiSI	CCGG	2 cut(s)	127, 2291
BsiWI	CGTACG	1 cut(s)	935
BslFI	GGGAC	7 cut(s)	53, 181, 604, 1016, 1185, 1649, 2273
BslI	CCNNNNNNNGG	3 cut(s)	444, 553, 1039
BsmAI	GTCTC	2 cut(s)	708, 1946
BsmFI	GGGAC	7 cut(s)	53, 181, 604, 1016, 1185, 1649, 2273
BsmI	GAATGC	2 cut(s)	752, 1402
BsnI	GGCC	3 cut(s)	1348, 2130, 2430
Bso31I	GGTCTC	1 cut(s)	1946
Bsp1286I	GDGCHC	3 cut(s)	421, 1386, 1936
Bsp1407I	TGTACA	1 cut(s)	1910
Bsp143I	GATC	5 cut(s)	176, 399, 1566, 2053, 2368
Bsp1720I	GCTNAGC	2 cut(s)	1203, 1521
Bsp19I	CCATGG	1 cut(s)	462
BspACI	CCGC	8 cut(s)	71, 86, 89, 92, 98, 274, 375, 434
BspANI	GGCC	3 cut(s)	1348, 2130, 2430
BspCNI	CTCAG	4 cut(s)	91, 1195, 1277, 1399
BspDI	ATCGAT	2 cut(s)	115, 402
BspFNI	CGCG	1 cut(s)	73
BspHI	TCATGA	2 cut(s)	202, 2208
BspLI	GGNNCC	1 cut(s)	1933
BspMAI	CTGCAG	2 cut(s)	1170, 1295
BspMI	ACCTGC	2 cut(s)	1198, 1284
BspPI	GGATC	1 cut(s)	1574
BspTNI	GGTCTC	1 cut(s)	1946
BsrBI	CCGCTC	1 cut(s)	377
BsrDI	GCAATG	3 cut(s)	415, 816, 2032
BsrGI	TGTACA	1 cut(s)	1910
BsrI	ACTGG	6 cut(s)	547, 930, 1230, 1350, 1563, 1643
BssECI	CCNNGG	2 cut(s)	462, 1936
BssMI	GATC	5 cut(s)	176, 399, 1566, 2053, 2368
BssSI	CACGAG	1 cut(s)	705
BssT1I	CCWWGG	2 cut(s)	462, 1936
Bst2BI	CACGAG	1 cut(s)	705
Bst2UI	CCWGG	1 cut(s)	1040
Bst4CI	ACNGT	4 cut(s)	542, 686, 1910, 2206
BstAPI	GCANNNNNTGC	1 cut(s)	1290
BstAUI	TGTACA	1 cut(s)	1910
BstBAI	YACGTR	1 cut(s)	2199
BstC8I	GCNNGC	3 cut(s)	417, 1200, 2432
BstDEI	CTNAG	9 cut(s)	78, 1088, 1203, 1213, 1264, 1407, 1521, 1631, 1857
BstDSI	CCRYGG	1 cut(s)	462
BstF5I	GGATG	7 cut(s)	727, 1117, 1647, 1726, 2016, 2070, 2299
BstFNI	CGCG	1 cut(s)	73
BstHHI	GCGC	1 cut(s)	952
BstKTI	GATC	5 cut(s)	179, 402, 1569, 2056, 2371
BstMAI	GTCTC	2 cut(s)	708, 1946
BstMBI	GATC	5 cut(s)	176, 399, 1566, 2053, 2368
BstNI	CCWGG	1 cut(s)	1040
BstNSI	RCATGY	4 cut(s)	452, 1202, 1510, 2451
BstSCI	CCNGG	3 cut(s)	126, 1038, 2289
BstSFI	CTRYAG	4 cut(s)	1166, 1242, 1291, 1865
BstSNI	TACGTA	1 cut(s)	2199
BstUI	CGCG	1 cut(s)	73
BstV1I	GCAGC	3 cut(s)	50, 487, 1463
BstV2I	GAAGAC	1 cut(s)	2160
BstX2I	RGATCY	2 cut(s)	176, 1566
BstYI	RGATCY	2 cut(s)	176, 1566
Bsu15I	ATCGAT	2 cut(s)	115, 402
BsuI	GTATCC	2 cut(s)	142, 174
BsuRI	GGCC	3 cut(s)	1348, 2130, 2430
BsuTUI	ATCGAT	2 cut(s)	115, 402
BtgI	CCRYGG	1 cut(s)	462
BtsCI	GGATG	7 cut(s)	727, 1117, 1647, 1726, 2016, 2070, 2299
BtsIMutI	CAGTG	4 cut(s)	1097, 1307, 1357, 2202
BveI	ACCTGC	2 cut(s)	1198, 1284
Cac8I	GCNNGC	3 cut(s)	417, 1200, 2432
CciI	TCATGA	2 cut(s)	202, 2208
CfoI	GCGC	1 cut(s)	952
Cfr13I	GGNCC	2 cut(s)	124, 1347
ClaI	ATCGAT	2 cut(s)	115, 402
CseI	GACGC	2 cut(s)	55, 62
CsiI	ACCWGGT	1 cut(s)	1038
Csp6I	GTAC	7 cut(s)	543, 936, 1556, 1801, 1911, 1983, 2200
CspCI	CAANNNNNGTGG	2 cut(s)	1238, 1273
CviQI	GTAC	7 cut(s)	543, 936, 1556, 1801, 1911, 1983, 2200
DdeI	CTNAG	9 cut(s)	78, 1088, 1203, 1213, 1264, 1407, 1521, 1631, 1857
DpnI	GATC	5 cut(s)	178, 401, 1568, 2055, 2370
DpnII	GATC	5 cut(s)	176, 399, 1566, 2053, 2368
EciI	GGCGGA	1 cut(s)	75
Eco105I	TACGTA	1 cut(s)	2199
Eco130I	CCWWGG	2 cut(s)	462, 1936
Eco24I	GRGCYC	2 cut(s)	1386, 1936
Eco31I	GGTCTC	1 cut(s)	1946
Eco32I	GATATC	1 cut(s)	523
Eco47I	GGWCC	1 cut(s)	124
Eco57I	CTGAAG	2 cut(s)	494, 1327
EcoRI	GAATTC	1 cut(s)	646
EcoRII	CCWGG	1 cut(s)	1038
EcoRV	GATATC	1 cut(s)	523
EcoT14I	CCWWGG	2 cut(s)	462, 1936
EcoT22I	ATGCAT	1 cut(s)	1695
EcoT38I	GRGCYC	2 cut(s)	1386, 1936
ErhI	CCWWGG	2 cut(s)	462, 1936
FalI	AAGNNNNNCTT	2 cut(s)	2034, 2066
FaqI	GGGAC	7 cut(s)	53, 181, 604, 1016, 1185, 1649, 2273
FauI	CCCGC	4 cut(s)	78, 105, 382, 441
FauNDI	CATATG	1 cut(s)	1780
FbaI	TGATCA	2 cut(s)	2053, 2368
FblI	GTMKAC	1 cut(s)	1245
Fnu4HI	GCNGC	5 cut(s)	64, 89, 92, 501, 1452
FokI	GGATG	7 cut(s)	734, 1124, 1654, 1733, 2023, 2077, 2306
FriOI	GRGCYC	2 cut(s)	1386, 1936
Fsp4HI	GCNGC	5 cut(s)	64, 89, 92, 501, 1452
FspBI	CTAG	4 cut(s)	798, 812, 2126, 2231
GlaI	GCGC	1 cut(s)	951
GluI	GCNGC	5 cut(s)	64, 89, 92, 501, 1452
GsuI	CTGGAG	1 cut(s)	277
HaeIII	GGCC	3 cut(s)	1348, 2130, 2430
HapII	CCGG	2 cut(s)	127, 2291
HgaI	GACGC	2 cut(s)	55, 62
HhaI	GCGC	1 cut(s)	952
Hin6I	GCGC	1 cut(s)	950
HinP1I	GCGC	1 cut(s)	950
HindIII	AAGCTT	3 cut(s)	1620, 1628, 2352
HinfI	GANTC	8 cut(s)	391, 557, 894, 925, 1026, 1301, 1480, 1496
HpaII	CCGG	2 cut(s)	127, 2291
HphI	GGTGA	3 cut(s)	1798, 1841, 1864
Hpy166II	GTNNAC	7 cut(s)	347, 727, 735, 1246, 1832, 2202, 2228
Hpy188I	TCNGA	8 cut(s)	105, 390, 475, 493, 893, 1408, 1479, 1901
Hpy188III	TCNNGA	8 cut(s)	203, 294, 625, 719, 798, 1335, 2051, 2209
Hpy8I	GTNNAC	7 cut(s)	347, 727, 735, 1246, 1832, 2202, 2228
HpyAV	CCTTC	7 cut(s)	6, 49, 1000, 1957, 2057, 2063, 2335
HpyCH4III	ACNGT	4 cut(s)	542, 686, 1910, 2206
HpyCH4IV	ACGT	2 cut(s)	1251, 2198
HpyF3I	CTNAG	9 cut(s)	78, 1088, 1203, 1213, 1264, 1407, 1521, 1631, 1857
HpySE526I	ACGT	2 cut(s)	1251, 2198
HspAI	GCGC	1 cut(s)	950
Ksp22I	TGATCA	2 cut(s)	2053, 2368
Kzo9I	GATC	5 cut(s)	176, 399, 1566, 2053, 2368
LmnI	GCTCC	4 cut(s)	41, 826, 957, 1931
Lsp1109I	GCAGC	3 cut(s)	50, 487, 1463
LweI	GCATC	7 cut(s)	769, 979, 1212, 1714, 2026, 2284, 2443
MabI	ACCWGGT	1 cut(s)	1038
MaeI	CTAG	4 cut(s)	798, 812, 2126, 2231
MaeII	ACGT	2 cut(s)	1251, 2198
MaeIII	GTNAC	6 cut(s)	832, 1147, 1502, 1852, 1949, 2299
MalI	GATC	5 cut(s)	178, 401, 1568, 2055, 2370
MbiI	CCGCTC	1 cut(s)	377
MboI	GATC	5 cut(s)	176, 399, 1566, 2053, 2368
MfeI	CAATTG	2 cut(s)	783, 1320
MflI	RGATCY	2 cut(s)	176, 1566
MhlI	GDGCHC	3 cut(s)	421, 1386, 1936
Mph1103I	ATGCAT	1 cut(s)	1695
MroXI	GAANNNNTTC	2 cut(s)	16, 1402
MseI	TTAA	9 cut(s)	44, 59, 303, 366, 957, 969, 1155, 1731, 2315
MslI	CAYNNNNRTG	2 cut(s)	467, 2207
MspI	CCGG	2 cut(s)	127, 2291
MspR9I	CCNGG	3 cut(s)	128, 1040, 2291
MunI	CAATTG	2 cut(s)	783, 1320
Mva1269I	GAATGC	2 cut(s)	752, 1402
MvaI	CCWGG	1 cut(s)	1040
MvnI	CGCG	1 cut(s)	73
NciI	CCSGG	2 cut(s)	128, 2291
NcoI	CCATGG	1 cut(s)	462
NdeI	CATATG	1 cut(s)	1780
NdeII	GATC	5 cut(s)	176, 399, 1566, 2053, 2368
NlaIV	GGNNCC	1 cut(s)	1933
NmuCI	GTSAC	3 cut(s)	1852, 1949, 2299
NsiI	ATGCAT	1 cut(s)	1695
NspI	RCATGY	4 cut(s)	452, 1202, 1510, 2451
PaeI	GCATGC	1 cut(s)	1202
PagI	TCATGA	2 cut(s)	202, 2208
PciI	ACATGT	1 cut(s)	2447
PctI	GAATGC	2 cut(s)	752, 1402
PdmI	GAANNNNTTC	2 cut(s)	16, 1402
PfeI	GAWTC	8 cut(s)	391, 557, 894, 925, 1026, 1301, 1480, 1496
Pfl23II	CGTACG	1 cut(s)	935
PfoI	TCCNGGA	1 cut(s)	2289
PkrI	GCNGC	5 cut(s)	65, 90, 93, 502, 1453
Ppu21I	YACGTR	1 cut(s)	2199
PscI	ACATGT	1 cut(s)	2447
PsiI	TTATAA	1 cut(s)	1887
Psp6I	CCWGG	1 cut(s)	1038
PspGI	CCWGG	1 cut(s)	1038
PspLI	CGTACG	1 cut(s)	935
PspN4I	GGNNCC	1 cut(s)	1933
PspPI	GGNCC	2 cut(s)	124, 1347
PsrI	GAACNNNNNNTAC	2 cut(s)	2136, 2168
PstI	CTGCAG	2 cut(s)	1170, 1295
PsuI	RGATCY	2 cut(s)	176, 1566
RsaI	GTAC	7 cut(s)	544, 937, 1557, 1802, 1912, 1984, 2201
RsaNI	GTAC	7 cut(s)	543, 936, 1556, 1801, 1911, 1983, 2200
RseI	CAYNNNNRTG	2 cut(s)	467, 2207
SaqAI	TTAA	9 cut(s)	44, 59, 303, 366, 957, 969, 1155, 1731, 2315
SatI	GCNGC	5 cut(s)	64, 89, 92, 501, 1452
Sau3AI	GATC	5 cut(s)	176, 399, 1566, 2053, 2368
Sau96I	GGNCC	2 cut(s)	124, 1347
ScrFI	CCNGG	3 cut(s)	128, 1040, 2291
SduI	GDGCHC	3 cut(s)	421, 1386, 1936
SexAI	ACCWGGT	1 cut(s)	1038
SfaNI	GCATC	7 cut(s)	769, 979, 1212, 1714, 2026, 2284, 2443
SfcI	CTRYAG	4 cut(s)	1166, 1242, 1291, 1865
SinI	GGWCC	1 cut(s)	124
SmiMI	CAYNNNNRTG	2 cut(s)	467, 2207
SmlI	CTYRAG	2 cut(s)	623, 1529
SmoI	CTYRAG	2 cut(s)	623, 1529
SnaBI	TACGTA	1 cut(s)	2199
SphI	GCATGC	1 cut(s)	1202
SsiI	CCGC	8 cut(s)	71, 86, 89, 92, 98, 274, 375, 434
SspI	AATATT	1 cut(s)	2020
SspMI	CTAG	4 cut(s)	798, 812, 2126, 2231
StyD4I	CCNGG	3 cut(s)	126, 1038, 2289
StyI	CCWWGG	2 cut(s)	462, 1936
TaaI	ACNGT	4 cut(s)	542, 686, 1910, 2206
TaiI	ACGT	2 cut(s)	1254, 2201
TaqI	TCGA	4 cut(s)	115, 210, 379, 402
TatI	WGTACW	1 cut(s)	1910
TauI	GCSGC	2 cut(s)	91, 94
TfiI	GAWTC	8 cut(s)	391, 557, 894, 925, 1026, 1301, 1480, 1496
Tru1I	TTAA	9 cut(s)	44, 59, 303, 366, 957, 969, 1155, 1731, 2315
Tru9I	TTAA	9 cut(s)	44, 59, 303, 366, 957, 969, 1155, 1731, 2315
TscAI	CASTG	4 cut(s)	1104, 1314, 1357, 2209
TseFI	GTSAC	3 cut(s)	1852, 1949, 2299
TseI	GCWGC	3 cut(s)	63, 500, 1451
Tsp45I	GTSAC	3 cut(s)	1852, 1949, 2299
TspDTI	ATGAA	8 cut(s)	521, 842, 1472, 1867, 2099, 2160, 2285, 2415
TspGWI	ACGGA	1 cut(s)	606
TspRI	CASTG	4 cut(s)	1104, 1314, 1357, 2209
VpaK11BI	GGWCC	1 cut(s)	124
XapI	RAATTY	5 cut(s)	646, 1141, 1313, 1656, 1873
XbaI	TCTAGA	1 cut(s)	797
XceI	RCATGY	4 cut(s)	452, 1202, 1510, 2451
XmiI	GTMKAC	1 cut(s)	1245
XmnI	GAANNNNTTC	2 cut(s)	16, 1402
XspI	CTAG	4 cut(s)	798, 812, 2126, 2231
Zsp2I	ATGCAT	1 cut(s)	1695

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.