Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0003055

Physical Location & Seq

Genomic Coordinates Forward (+)

38485506 .. 38487845

Gene Structure 2340 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000002716

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2340 bp

ATGGATTTGAGAAGGCTTCCGATCTCCATACCCGCTTCTCTCTTCTACCCAATCACACGCCCCTTAACGGGCGTGACATTCACCTTCCAACGCCGCCACGACCCTCCCCAGCCGCCGCCGCCGTTACAGCCTCCGAGTCCACCACAGCCTCCCAATCCGGGTTTGCTCAAATGGGTATCTTCTATTCTCTCATACCCATCTTTGGATTCTTCTAAATGTGAATCTCTCATACCCCTTTTGTCCCCTCATGAATTTGATCAGTTATTCTACTCCATTAGGTCCAATGTGAACCCCAAAACAGTCCTTCATTTCTTTCATTTTGCTTCTCAGTCTTTTAAGTTTCGGTTTACCGTTCGATCTTTCTGTGTTTTGGTTCATTTGCTCATTGCTTCAAATCTTGGGACTCCTGCGAGGTTGCTTTTGATTCGTTTGATTGATGGGAATGTCCCAGTTTTGTGTGCTGACCCCAACAGCAAGCATATTGAGATAGCCGTTGCAATGTTGGAGTTGAACAATGTGTCCCAACCGGCTCTGGGGATTCAGGCATTGGATATGTTAATTCATGTTTACTGTACCCAGTTCAAGAATTTGGGTTTTGGTTATGCTGTTGATGTGTTTGAGCATTTTTCGGATAAGGGTCTGTTTCCGTCTTTGAAGACTTGTAATGTTTTGCTGAGTTCTTTAGTGAAGGCTAACGAACTTGAGAAGAGCTATCATGTATTTGAAGTTATGACTCGAGGTGTTTGTCCTGATGTTTTCTTGTTTACTACTGCAATTAATGCATTTTGCAAGGGAGGGAAGGTGGATTATGCTATAGCTGTGTTCTCAAAAATGGAGAGCATTGGTATTGGTCCGAATTTTGTTACTTACAATAGCGTTATCCACGGGCTATGTAAGAGTAGAAGATTAGAGGAGGCTTTCCAGTTTAAGGAGAAGATGGTAAAGAATAATGTGAAGCCGAGTCTTATAACATATAGTGTGCTCATTAATGGTTTGATTAAGCTGGAGAAATTTTATGAGGCAAATTGTGTTTTCAAGGAAATGCGTAGTAGTGGTTTTGTCCCGAATGAGGTTGTATATAACACTCTGATTGATGGGTATTGTACAATGGGAAATATTGGCGAGGCACTCAAGATAAGAGATGATATGGTATCGAATGGGGTTACCCCTAATTCTGTTACTCTCAATTCGCTACTGCATGGATTTTGTAAAAGTAATCAATTTGAGCATGCTGAGCAATTTTTGGACAAGATGCTAGCCAGTGGTTTACCGGTCAACCAAGTTGTTTCTTACTCAGTGATTGACTGGTTATGCATGAAATCTAGGTTTGATTCTGCATTAAAATTCACTACTGAAATGTTATTAAGAAACTTTAGGCCCAGTGATAGTTTACTTACTACATTGTTTGTTGGGCTCTGCAAAGATGGGAAGAATTTAGAGGCAATCGAACTTTGGTTTAGGCTATCGGAGAAAGGGTTTGCAGCCAACACAGCGACCTCAAATGCTCTAATCCATGGACTTTGTGAATCTGGTAGCAACGAAGAGGTTGTTAGGCTACTCAAGGCTATACTTGAGAGGGGTATGGCATTGGATAAGTTCTCATGCAACACACTCATTTTAAGTTGTTGCAAGGAGGGAAGAGTAGCGGAAGGTTTTAAGCTTAGGGAAGAGATGTCTAAGCAAGGAATTGATCCTGACATCAACACTTACAATATGCTACTGCATGGTCTATGTAGTATTGGCAAAGTGGACGATGCTGTTAAACTTTGGGTTGAATGCGAAAATCATGGTCTTGTGGCCAATGTTTATACGTATGAGGTGATGATAGATGGGTACTGTAAATCTGGTCGAATCAAAGACGCTGAACTCCTGTTTAGTAAGTTGGTGACTAAGAAAGTGGAGCCAAATTCCGTTGTTTATAATACACTAATCAGTGCATATTGTATAGATGGGAATATGATTGGAGCTCTTGGCCTATTCCATGACATGAAAAACAAGGGCATTCAACCAACTTATGCCACATATTTATCTCTTATAAATAGGCTATGCAATATCGGCAATGTCGAGGATGCAAAAGAGCTCATTGATGAAATGAGGAGGAATGATTTGGTGCCAAATATCGCTTGTTATGGTGAAGTGATTTGTGGTTATTGTAAGTTAGGCCAAATGGATAAAGCAAGGAGCATTTTGCTGGAGATGTCCTCATATAACATACAACCGAATAAAATTACATATACTGTCATGATTGATGGGTATTGCAAGCTAGGCAACATGGAAGAAAAAAATATACTTCAATGTGAGATGGCAAAAAAGGGAATTGTCCCAGATGCAGTCACTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

780

Amino Acids

87.05

Weight (kDa)

7.42

Isoelectric Point (pI)

37.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_long	PF17177	202 - 329	1.6e-08	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	242 - 295	1.5e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	246 - 279	2.1e-10	PPR repeat
PPR_2	PF13041	250 - 299	2.8e-13	PPR repeat family
PPR_1	PF12854	282 - 314	4.2e-11	PPR repeat
PPR_2	PF13041	285 - 334	1.9e-17	PPR repeat family
PPR	PF01535	288 - 318	2.7e-07	PPR repeat
PPR_3	PF13812	310 - 367	3.1e-07	Pentatricopeptide repeat domain
PPR_long	PF17177	339 - 425	6.6e-06	Pentacotripeptide-repeat region of PRORP
TPR_24	PF23276	344 - 427	7.7e-07	Fungal tetratrico peptide repeats
PPR_3	PF13812	344 - 400	7.1e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	352 - 384	1.1e-10	PPR repeat
PPR_2	PF13041	355 - 404	1.9e-17	PPR repeat family
PPR	PF01535	358 - 388	2.2e-07	PPR repeat
PPR_1	PF12854	386 - 418	2.5e-09	PPR repeat
PPR_2	PF13041	390 - 428	3.6e-08	PPR repeat family
PPR	PF01535	393 - 423	9.3e-06	PPR repeat
PPR_2	PF13041	466 - 508	9.6e-06	PPR repeat family
PPR_2	PF13041	532 - 578	3.2e-15	PPR repeat family
PPR_1	PF12854	562 - 591	1.1e-09	PPR repeat
PPR_2	PF13041	565 - 614	1.8e-14	PPR repeat family
PPR_3	PF13812	590 - 647	1.4e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	597 - 627	2.8e-09	PPR repeat
PPR_long	PF17177	614 - 757	5.2e-10	Pentacotripeptide-repeat region of PRORP
TPR_24	PF23276	628 - 726	6.9e-08	Fungal tetratrico peptide repeats
PPR_1	PF12854	632 - 664	9.3e-08	PPR repeat
PPR_2	PF13041	635 - 684	2e-15	PPR repeat family
PPR	PF01535	638 - 668	1.7e-08	PPR repeat
PPR_3	PF13812	659 - 714	1.8e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	666 - 699	1.9e-07	PPR repeat
PPR_2	PF13041	674 - 710	7.3e-06	PPR repeat family
PPR_2	PF13041	705 - 754	3.6e-15	PPR repeat family
PPR_1	PF12854	737 - 760	1.7e-06	PPR repeat
PPR_2	PF13041	745 - 779	3.1e-06	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003055)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G19440 AT4G19440 AT4G19440 AT4G19440
fragaria_vesca	FvH4_4g10410 FvH4_4g10410 FvH4_4g10440 FvH4_5g24430 FvH4_5g24430 FvH4_5g24430 FvH4_5g24430
malus_domestica	MD06G1022200.v1.1
prunus_persica	Prupe.3G018200_v2.0.a1
pyrus_communis	pycom06g01610
rosa_chinensis	RchiOBHm_Chr4g0405931 RchiOBHm_Chr7g0214821 RchiOBHm_Chr7g0219861
rosa_laevigata	RLG00000002716 RLG00000008810
rosa_multiflora	Rmu_sc0002308.1_g000021 Rmu_sc0019702.1_g000004 Rmu_ssc0000087.1_g000071
rosa_roxburghii	Rroxscaffold_3G00244980 Rroxscaffold_5G00350670
rosa_rugosa	Rorug04G0064100 Rorug07G0147600 Rorug07G0147700 Rorug07G0147800
rosa_samantha	Rh4AG134900 Rh4BG130500 Rh4CG142300 Rh4DG129200 Rh7AG284500 Rh7BG275300 Rh7CG303700 Rh7DG287900
rosa_wichuraiana	Rw4G010980 Rw7G024260

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	968, 1920, 2036
AccB1I	GGYRCC	1 cut(s)	2110
AciI	CCGC	6 cut(s)	33, 94, 113, 116, 119, 1646
AclWI	GGATC	1 cut(s)	1685
AcoI	YGGCCR	1 cut(s)	1797
AcsI	RAATTY	7 cut(s)	251, 586, 856, 1010, 1343, 1432, 1906
AfaI	GTAC	3 cut(s)	574, 1105, 1835
AfiI	CCNNNNNNNGG	7 cut(s)	67, 68, 158, 202, 533, 928, 1069
AgeI	ACCGGT	1 cut(s)	1270
AgsI	TTSAA	9 cut(s)	393, 511, 583, 655, 725, 1036, 1775, 2006, 2294
AloI	GAACNNNNNNTCC	2 cut(s)	503, 535
AluBI	AGCT	7 cut(s)	711, 818, 1003, 1660, 1967, 2080, 2263
AluI	AGCT	7 cut(s)	711, 818, 1003, 1660, 1967, 2080, 2263
Alw21I	GWGCWC	3 cut(s)	984, 1969, 2082
AlwI	GGATC	1 cut(s)	1685
Ama87I	CYCGRG	1 cut(s)	735
AoxI	GGCC	4 cut(s)	1376, 1797, 1972, 2161
ApeKI	GCWGC	1 cut(s)	1481
ApoI	RAATTY	7 cut(s)	251, 586, 856, 1010, 1343, 1432, 1906
AseI	ATTAAT	2 cut(s)	777, 987
AsiGI	ACCGGT	1 cut(s)	1270
AspS9I	GGNCC	3 cut(s)	279, 851, 1377
AsuC2I	CCSGG	1 cut(s)	159
AsuHPI	GGTGA	4 cut(s)	73, 1831, 1897, 2144
AsuNHI	GCTAGC	1 cut(s)	1255
AvaI	CYCGRG	1 cut(s)	735
AvaII	GGWCC	2 cut(s)	279, 851
BalI	TGGCCA	1 cut(s)	1799
BanI	GGYRCC	1 cut(s)	2110
BanII	GRGCYC	3 cut(s)	1416, 1969, 2082
BbsI	GAAGAC	1 cut(s)	662
Bbv12I	GWGCWC	3 cut(s)	984, 1969, 2082
BbvI	GCAGC	1 cut(s)	1493
BccI	CCATC	9 cut(s)	205, 431, 931, 1088, 1418, 1823, 1943, 2243, 2296
BceAI	ACGGC	2 cut(s)	106, 476
BclI	TGATCA	1 cut(s)	256
BcnI	CCSGG	1 cut(s)	159
BfaI	CTAG	3 cut(s)	1256, 1323, 2264
BfmI	CTRYAG	1 cut(s)	813
BisI	GCNGC	5 cut(s)	94, 113, 116, 119, 1482
BlpI	GCTNAGC	1 cut(s)	1233
BlsI	GCNGC	5 cut(s)	95, 114, 117, 120, 1483
Bme1390I	CCNGG	1 cut(s)	159
Bme18I	GGWCC	2 cut(s)	279, 851
BmeT110I	CYCGRG	1 cut(s)	735
BmgT120I	GGNCC	3 cut(s)	279, 851, 1377
BmiI	GGNNCC	2 cut(s)	1902, 2112
BmrFI	CCNGG	1 cut(s)	159
BmrI	ACTGGG	3 cut(s)	443, 571, 1374
BmsI	GCATC	4 cut(s)	1242, 1744, 2059, 2317
BmtI	GCTAGC	1 cut(s)	1259
BmuI	ACTGGG	3 cut(s)	443, 571, 1374
BpiI	GAAGAC	1 cut(s)	662
BplI	GAGNNNNNCTC	2 cut(s)	2186, 2218
BpmI	CTGGAG	2 cut(s)	1025, 2213
Bpu10I	CCTNAGC	1 cut(s)	1661
Bpu1102I	GCTNAGC	1 cut(s)	1233
BpuEI	CTTGAG	4 cut(s)	722, 1115, 1544, 1592
BpuMI	CCSGG	1 cut(s)	159
BsaAI	YACGTR	1 cut(s)	1812
BsaJI	CCNNGG	2 cut(s)	883, 1513
BsaWI	WCCGGW	1 cut(s)	1270
BsaXI	ACNNNNNCTCC	4 cut(s)	786, 816, 1460, 1490
Bsc4I	CCNNNNNNNGG	7 cut(s)	67, 68, 158, 202, 533, 928, 1069
Bse118I	RCCGGY	2 cut(s)	526, 1270
Bse1I	ACTGG	6 cut(s)	449, 577, 922, 1260, 1310, 1380
Bse3DI	GCAATG	3 cut(s)	384, 504, 2065
BseDI	CCNNGG	2 cut(s)	883, 1513
BseGI	GGATG	1 cut(s)	2074
BseLI	CCNNNNNNNGG	7 cut(s)	67, 68, 158, 202, 533, 928, 1069
BseMI	GCAATG	3 cut(s)	384, 504, 2065
BseMII	CTCAG	4 cut(s)	341, 665, 1224, 1308
BseNI	ACTGG	6 cut(s)	449, 577, 922, 1260, 1310, 1380
BseRI	GAGGAG	2 cut(s)	926, 2110
BseXI	GCAGC	1 cut(s)	1493
BseYI	CCCAGC	1 cut(s)	108
BshFI	GGCC	4 cut(s)	1378, 1799, 1974, 2163
BshNI	GGYRCC	1 cut(s)	2110
BshTI	ACCGGT	1 cut(s)	1270
BsiHKAI	GWGCWC	3 cut(s)	984, 1969, 2082
BsiHKCI	CYCGRG	1 cut(s)	735
BsiSI	CCGG	3 cut(s)	158, 527, 1271
BslFI	GGGAC	6 cut(s)	226, 415, 431, 505, 1046, 2306
BslI	CCNNNNNNNGG	7 cut(s)	67, 68, 158, 202, 533, 928, 1069
BsmFI	GGGAC	6 cut(s)	226, 415, 431, 505, 1046, 2306
BsmI	GAATGC	2 cut(s)	1781, 2001
BsnI	GGCC	4 cut(s)	1378, 1799, 1974, 2163
BsoBI	CYCGRG	1 cut(s)	735
Bsp1286I	GDGCHC	4 cut(s)	984, 1416, 1969, 2082
Bsp1407I	TGTACA	1 cut(s)	1103
Bsp143I	GATC	4 cut(s)	21, 256, 356, 1690
Bsp1720I	GCTNAGC	1 cut(s)	1233
Bsp19I	CCATGG	1 cut(s)	1513
BspACI	CCGC	6 cut(s)	33, 94, 113, 116, 119, 1646
BspANI	GGCC	4 cut(s)	1378, 1799, 1974, 2163
BspCNI	CTCAG	4 cut(s)	340, 666, 1225, 1307
BspHI	TCATGA	2 cut(s)	247, 2241
BspLI	GGNNCC	2 cut(s)	1902, 2112
BspOI	GCTAGC	1 cut(s)	1259
BspPI	GGATC	1 cut(s)	1685
BspQI	GCTCTTC	1 cut(s)	701
BspT107I	GGYRCC	1 cut(s)	2110
BsrDI	GCAATG	3 cut(s)	384, 504, 2065
BsrFI	RCCGGY	2 cut(s)	526, 1270
BsrGI	TGTACA	1 cut(s)	1103
BsrI	ACTGG	6 cut(s)	449, 577, 922, 1260, 1310, 1380
BssAI	RCCGGY	2 cut(s)	526, 1270
BssECI	CCNNGG	2 cut(s)	883, 1513
BssMI	GATC	4 cut(s)	21, 256, 356, 1690
BssT1I	CCWWGG	1 cut(s)	1513
Bst4CI	ACNGT	5 cut(s)	301, 352, 572, 1838, 2239
Bst6I	CTCTTC	5 cut(s)	47, 701, 1536, 1633, 1662
BstAPI	GCANNNNNTGC	1 cut(s)	779
BstAUI	TGTACA	1 cut(s)	1103
BstBAI	YACGTR	1 cut(s)	1812
BstC8I	GCNNGC	4 cut(s)	476, 1230, 1257, 2261
BstDEI	CTNAG	8 cut(s)	327, 674, 1233, 1294, 1661, 1677, 1890, 2337
BstDSI	CCRYGG	2 cut(s)	883, 1513
BstEII	GGTNACC	1 cut(s)	1162
BstF5I	GGATG	1 cut(s)	2074
BstKTI	GATC	4 cut(s)	24, 259, 359, 1693
BstMBI	GATC	4 cut(s)	21, 256, 356, 1690
BstMWI	GCNNNNNNNGC	7 cut(s)	118, 127, 779, 1234, 1490, 2055, 2265
BstNSI	RCATGY	1 cut(s)	1232
BstPI	GGTNACC	1 cut(s)	1162
BstSCI	CCNGG	1 cut(s)	157
BstSFI	CTRYAG	1 cut(s)	813
BstSNI	TACGTA	1 cut(s)	1812
BstV1I	GCAGC	1 cut(s)	1493
BstV2I	GAAGAC	1 cut(s)	662
BsuRI	GGCC	4 cut(s)	1378, 1799, 1974, 2163
BtgI	CCRYGG	2 cut(s)	883, 1513
BtsCI	GGATG	1 cut(s)	2074
BtsIMutI	CAGTG	4 cut(s)	1267, 1302, 1387, 1939
Cac8I	GCNNGC	4 cut(s)	476, 1230, 1257, 2261
CciI	TCATGA	2 cut(s)	247, 2241
Cfr10I	RCCGGY	2 cut(s)	526, 1270
Cfr13I	GGNCC	3 cut(s)	279, 851, 1377
CseI	GACGC	1 cut(s)	1868
Csp6I	GTAC	3 cut(s)	573, 1104, 1834
CspAI	ACCGGT	1 cut(s)	1270
CviQI	GTAC	3 cut(s)	573, 1104, 1834
DdeI	CTNAG	8 cut(s)	327, 674, 1233, 1294, 1661, 1677, 1890, 2337
DpnI	GATC	4 cut(s)	23, 258, 358, 1692
DpnII	GATC	4 cut(s)	21, 256, 356, 1690
EaeI	YGGCCR	1 cut(s)	1797
Eam1104I	CTCTTC	5 cut(s)	47, 701, 1536, 1633, 1662
EarI	CTCTTC	5 cut(s)	47, 701, 1536, 1633, 1662
Ecl136II	GAGCTC	2 cut(s)	1967, 2080
Eco105I	TACGTA	1 cut(s)	1812
Eco130I	CCWWGG	1 cut(s)	1513
Eco24I	GRGCYC	3 cut(s)	1416, 1969, 2082
Eco47I	GGWCC	2 cut(s)	279, 851
Eco53kI	GAGCTC	2 cut(s)	1967, 2080
Eco88I	CYCGRG	1 cut(s)	735
Eco91I	GGTNACC	1 cut(s)	1162
EcoICRI	GAGCTC	2 cut(s)	1967, 2080
EcoO65I	GGTNACC	1 cut(s)	1162
EcoT14I	CCWWGG	1 cut(s)	1513
EcoT22I	ATGCAT	2 cut(s)	784, 1316
EcoT38I	GRGCYC	3 cut(s)	1416, 1969, 2082
ErhI	CCWWGG	1 cut(s)	1513
FaqI	GGGAC	6 cut(s)	226, 415, 431, 505, 1046, 2306
FauI	CCCGC	1 cut(s)	40
FbaI	TGATCA	1 cut(s)	256
Fnu4HI	GCNGC	5 cut(s)	94, 113, 116, 119, 1482
FokI	GGATG	1 cut(s)	2081
FriOI	GRGCYC	3 cut(s)	1416, 1969, 2082
Fsp4HI	GCNGC	5 cut(s)	94, 113, 116, 119, 1482
FspBI	CTAG	3 cut(s)	1256, 1323, 2264
GluI	GCNGC	5 cut(s)	94, 113, 116, 119, 1482
GsaI	CCCAGC	1 cut(s)	112
GsuI	CTGGAG	2 cut(s)	1025, 2213
HaeIII	GGCC	4 cut(s)	1378, 1799, 1974, 2163
HapII	CCGG	3 cut(s)	158, 527, 1271
HgaI	GACGC	1 cut(s)	1868
HincII	GTYRAC	1 cut(s)	1276
HindII	GTYRAC	1 cut(s)	1276
HindIII	AAGCTT	1 cut(s)	1658
HpaII	CCGG	3 cut(s)	158, 527, 1271
HphI	GGTGA	4 cut(s)	73, 1831, 1897, 2144
Hpy166II	GTNNAC	9 cut(s)	140, 289, 348, 568, 765, 1268, 1276, 1391, 1750
Hpy188I	TCNGA	6 cut(s)	21, 135, 631, 855, 1089, 1468
Hpy188III	TCNNGA	7 cut(s)	248, 583, 749, 1063, 1132, 1694, 2242
Hpy8I	GTNNAC	9 cut(s)	140, 289, 348, 568, 765, 1268, 1276, 1391, 1750
HpyAV	CCTTC	6 cut(s)	6, 94, 314, 682, 793, 1643
HpyCH4III	ACNGT	5 cut(s)	301, 352, 572, 1838, 2239
HpyCH4IV	ACGT	1 cut(s)	1811
HpyF10VI	GCNNNNNNNGC	7 cut(s)	118, 127, 779, 1234, 1490, 2055, 2265
HpyF3I	CTNAG	8 cut(s)	327, 674, 1233, 1294, 1661, 1677, 1890, 2337
HpySE526I	ACGT	1 cut(s)	1811
Ksp22I	TGATCA	1 cut(s)	256
Kzo9I	GATC	4 cut(s)	21, 256, 356, 1690
LguI	GCTCTTC	1 cut(s)	701
LmnI	GCTCC	3 cut(s)	1900, 1964, 2181
Lsp1109I	GCAGC	1 cut(s)	1493
LweI	GCATC	4 cut(s)	1242, 1744, 2059, 2317
MaeI	CTAG	3 cut(s)	1256, 1323, 2264
MaeII	ACGT	1 cut(s)	1811
MaeIII	GTNAC	7 cut(s)	73, 123, 862, 1162, 1177, 1885, 2332
MalI	GATC	4 cut(s)	23, 258, 358, 1692
MboI	GATC	4 cut(s)	21, 256, 356, 1690
MhlI	GDGCHC	4 cut(s)	984, 1416, 1969, 2082
MlsI	TGGCCA	1 cut(s)	1799
MluNI	TGGCCA	1 cut(s)	1799
MlyI	GAGTC	4 cut(s)	145, 397, 727, 970
MmeI	TCCRAC	2 cut(s)	112, 483
Mox20I	TGGCCA	1 cut(s)	1799
Mph1103I	ATGCAT	2 cut(s)	784, 1316
MscI	TGGCCA	1 cut(s)	1799
Msp20I	TGGCCA	1 cut(s)	1799
MspI	CCGG	3 cut(s)	158, 527, 1271
MspR9I	CCNGG	1 cut(s)	159
Mva1269I	GAATGC	2 cut(s)	1781, 2001
MwoI	GCNNNNNNNGC	7 cut(s)	118, 127, 779, 1234, 1490, 2055, 2265
NciI	CCSGG	1 cut(s)	159
NcoI	CCATGG	1 cut(s)	1513
NdeII	GATC	4 cut(s)	21, 256, 356, 1690
NheI	GCTAGC	1 cut(s)	1255
NlaIV	GGNNCC	2 cut(s)	1902, 2112
NmeAIII	GCCGAG	1 cut(s)	984
NmuCI	GTSAC	3 cut(s)	73, 1885, 2332
NsiI	ATGCAT	2 cut(s)	784, 1316
NspI	RCATGY	1 cut(s)	1232
PaeI	GCATGC	1 cut(s)	1232
PaeR7I	CTCGAG	1 cut(s)	735
PagI	TCATGA	2 cut(s)	247, 2241
PciSI	GCTCTTC	1 cut(s)	701
PcsI	WCGNNNNNNNCGW	1 cut(s)	2061
PctI	GAATGC	2 cut(s)	1781, 2001
PfeI	GAWTC	7 cut(s)	206, 221, 424, 538, 1331, 1526, 1851
PinAI	ACCGGT	1 cut(s)	1270
PkrI	GCNGC	5 cut(s)	95, 114, 117, 120, 1483
PleI	GAGTC	4 cut(s)	144, 397, 727, 969
PpsI	GAGTC	4 cut(s)	144, 397, 727, 969
Ppu21I	YACGTR	1 cut(s)	1812
PshBI	ATTAAT	2 cut(s)	777, 987
PsiI	TTATAA	3 cut(s)	968, 1920, 2036
Psp124BI	GAGCTC	2 cut(s)	1969, 2082
PspEI	GGTNACC	1 cut(s)	1162
PspFI	CCCAGC	1 cut(s)	108
PspN4I	GGNNCC	2 cut(s)	1902, 2112
PspPI	GGNCC	3 cut(s)	279, 851, 1377
PspXI	VCTCGAGB	1 cut(s)	735
RsaI	GTAC	3 cut(s)	574, 1105, 1835
RsaNI	GTAC	3 cut(s)	573, 1104, 1834
SacI	GAGCTC	2 cut(s)	1969, 2082
SapI	GCTCTTC	1 cut(s)	701
SatI	GCNGC	5 cut(s)	94, 113, 116, 119, 1482
Sau3AI	GATC	4 cut(s)	21, 256, 356, 1690
Sau96I	GGNCC	3 cut(s)	279, 851, 1377
SchI	GAGTC	4 cut(s)	145, 397, 727, 970
ScrFI	CCNGG	1 cut(s)	159
SduI	GDGCHC	4 cut(s)	984, 1416, 1969, 2082
SfaNI	GCATC	4 cut(s)	1242, 1744, 2059, 2317
SfcI	CTRYAG	1 cut(s)	813
Sfr274I	CTCGAG	1 cut(s)	735
SinI	GGWCC	2 cut(s)	279, 851
SlaI	CTCGAG	1 cut(s)	735
SmlI	CTYRAG	5 cut(s)	701, 735, 1130, 1559, 1571
SmoI	CTYRAG	5 cut(s)	701, 735, 1130, 1559, 1571
SnaBI	TACGTA	1 cut(s)	1812
SphI	GCATGC	1 cut(s)	1232
SsiI	CCGC	6 cut(s)	33, 94, 113, 116, 119, 1646
SspI	AATATT	1 cut(s)	1117
SspMI	CTAG	3 cut(s)	1256, 1323, 2264
SstI	GAGCTC	2 cut(s)	1969, 2082
StyD4I	CCNGG	1 cut(s)	157
StyI	CCWWGG	1 cut(s)	1513
TaaI	ACNGT	5 cut(s)	301, 352, 572, 1838, 2239
TaiI	ACGT	1 cut(s)	1814
TaqI	TCGA	6 cut(s)	355, 736, 1154, 1446, 1849, 2064
TatI	WGTACW	1 cut(s)	1103
TauI	GCSGC	4 cut(s)	96, 115, 118, 121
TfiI	GAWTC	7 cut(s)	206, 221, 424, 538, 1331, 1526, 1851
TscAI	CASTG	4 cut(s)	1267, 1302, 1387, 1939
TseFI	GTSAC	3 cut(s)	73, 1885, 2332
TseI	GCWGC	1 cut(s)	1481
Tsp45I	GTSAC	3 cut(s)	73, 1885, 2332
TspDTI	ATGAA	8 cut(s)	264, 296, 305, 365, 551, 1331, 2003, 2103
TspGWI	ACGGA	2 cut(s)	636, 1900
TspRI	CASTG	4 cut(s)	1267, 1302, 1387, 1939
VpaK11BI	GGWCC	2 cut(s)	279, 851
VspI	ATTAAT	2 cut(s)	777, 987
XapI	RAATTY	7 cut(s)	251, 586, 856, 1010, 1343, 1432, 1906
XceI	RCATGY	1 cut(s)	1232
XhoI	CTCGAG	1 cut(s)	735
XspI	CTAG	3 cut(s)	1256, 1323, 2264
Zsp2I	ATGCAT	2 cut(s)	784, 1316

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.