Rroxscaffold_5G00350670

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0003055
Physical Location & Seq
Reverse (-)
25015019 .. 25017478
2460 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00350670.1

Sequence Viewer

Length: 2460 bp
ATGGAGCTGAGAAGGGTTTCAATCTCGAAACCTTGCTCTTTCTTAATCAAACGCCCATTAAGCTGCGTCCCACGTCCTCAGCCTCCGGCGCCGCCGCCGAACAACCAGAGATTACTTGACCGGGTATCCTCTGTTCTCTCAAACTCATCTTTCAAGTACCCTCAATGTAGATCTCTCATATCCCTCTTGTCCCCTCATGAATTCGATGCCCTCTTCTATTCCGTCGCCGCTTCCCCCTCCTCCAATGTCAACCCCAAAACTGCTCTCCGTTTCTTTCACTTCGCTTCTAAAGCTTTCAACTTCCATTTCACCCTCGCCTCTTATTGCCTTTTGGTTCGTGTACTAATTCTTTCCAATCTTAACTCGCCTGCGAGATTGCTTTTGATCCGCTTGATTGATGGCAATGTGCAAGCTTTGTATTCTCTCCCGCCCACTGATAGGCACATTGAGATAGCCATGGCTATGTCCCACTTCAAAACCGCCTACGAAAGAGCTGCTGTGCTTCAGGCGTTGGATATGTTGATTCATCTCTACTGTACTCAGTTCAAGGATTTGGGTTTTTGTTCTGCCGTTCATGTTTTTGAGTTTTTCTCTAATAAGGCTATGTTTCCGTCCCTCAAGACTTGCCATTTTTTGTTGAATTCTTTAGTCAAGGCTGACCAACTTGATAAGAGCTACCGTGTGTTTGAAGTTCTGTTGCGTGGTGTTTCCCCGGATGTTTACTTGTTTACTACTGCAATTCAAGCATTTTGTAAGGGAGGGAAGGTTGATGATGCAATAGGTTTGTTCTCTAAAATGGAGCCACTTGGCATTGCTCCAAATGTTGTTACTTACAACAATGTTATTCACGCGTTATGTAAGAGCAGAAGATTAGAGGAGGCTTTCCAATTCAAGGACAAGATGGCACAAAACAATGTGAATCCCAGTCTGACAACATATGGTGTGCTCCTTAATGGTTTGATTAAGTTGGAGAAGTTTGACGACGCAAATCATGTTTTGAAGGAAATGTATAGTAAGGGATTTGTCCCCAATGAGGTTATCTATAACATATTGATTGACGGCTATTGTAAAACGGGAAGTACTAGTGAGGCACTGAAGTTTAGGGATGAAATGTTATCCAATGGGGTAACTCCAAATTCTGTTACCATTAACTCGCTACTGCAGGGATTTTGTAAAAGCAACCACTTTGAGCATGCTGAGCAGGTCTTAGATAAGGTGCTATCCAGTGGTTCATCGGTAGACCAAGTCGTTTGTTTCTCAGTCATTCACTCGTTATGCAAGAACTCTAGGTTTGATTCTGCACTGAAATTCACAATTGAAATGCTATTAAGAAACTTCAGGCCCAGCGATAACTTGCTTACAACATTGGTTGTTGGGCTCTGTAAAGATGGAAAGCATTCTGAGGCATCTCAACTTTGGTTTAGGCTATGGGAGAAAGGGTTTGCAGCTAACACAGTGACCTCAAATGCTCTAATTTATGGGGTTTGTGAATCTCGTAGCATGCAAGAGGTTGCTAGGCTACTCAAGGAAATGGTAAAGAGAGGCTTGGTATTGGATAGGATCTCATGCAACACACTCATCTTGGGTTGGTGCAAGGAGGGAAAAGTGGACGAAGGTTTTAAGATTAGGGACTGGATGGGTAAGAAAGGAATTTTGCCAGACACATATACTTACAATTTGCTAATGCATGGTCTATGTAATATGGGAAAAGTGGATGATGCTGTTAAACTTTGGGAGGAGTGCAGAAAACATGGTCTTGCTGATGTCTATACATATGGGGTGATGATAGATGGGTACTGTAGAGCTGACAGAATTAAAGACGGTGAAGACCTATTTATTGAGTTGGTGATTAAGAAACTACAGCTGAATTTCGTTGTTTATAATACGCTTATAAGAGCATACTGTACAAATGCGAACATGATTGGAGCCCTTGGTCTCTGCTGCGACATGAAAAAGAAGGGTATTGAACCAACGTGTACCACATATTCTTCTCTTATACATGGGATGTGCAATATTGGTAATGTCGAAGATGCAGAATACCTTCTTGATCAAATGAGGAAGGAGGGTTTGTTGCCCGATGTTGTTTGCTACACTGCACTAATTCATGGTTATTGTAAGCTAGGCCAGATGGATAAAGTAGGGAGTGTTCTTGGAGAGATGTCTTCATATAATGTACAACCTAATAAGATTACATACACTGTCATGATTGATGGGCATTGTAAACTAGGTAATATGGAAGAAGCAACTAAACTTCTATGCGAGATGGAAAAAAAGGGGATTGTCCCGGATGCTGTCACTTACAATGCCTTAACAAATGGATTTTGCAAGGAAAGGATGGTGGAAGAAGCTTTTGAAGTTTGTGATCAAATGTTCACTAAAGGTCTAGCTTTAGATGAAATTACCTATACGACATTGGTTAGTGGGCTGCATCAAACAGCTACATGTGCAGACAAGGAATAA

Protein Analysis

819

Amino Acids

91.85

Weight (kDa)

8.06

Isoelectric Point (pI)

31.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 179 - 318 4e-09 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 233 - 285 2.7e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 234 - 267 2.9e-10 PPR repeat
PPR_2 PF13041 238 - 279 2e-10 PPR repeat family
PPR_3 PF13812 262 - 318 1.4e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 270 - 301 1e-10 PPR repeat
PPR_2 PF13041 273 - 322 1e-15 PPR repeat family
PPR_3 PF13812 298 - 355 1.6e-06 Pentatricopeptide repeat domain
TPR_24 PF23276 330 - 436 9.4e-09 Fungal tetratrico peptide repeats
PPR_3 PF13812 332 - 389 9.9e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 339 - 371 4.9e-11 PPR repeat
PPR_2 PF13041 343 - 392 2.1e-17 PPR repeat family
PPR PF01535 346 - 376 2.9e-08 PPR repeat
PPR_1 PF12854 374 - 405 4.2e-06 PPR repeat
PPR_3 PF13812 506 - 563 7.9e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 520 - 567 5.1e-16 PPR repeat family
PPR_1 PF12854 549 - 580 3.3e-11 PPR repeat
PPR PF01535 556 - 586 1.6e-06 PPR repeat
PPR_2 PF13041 557 - 601 1.1e-12 PPR repeat family
PPR_1 PF12854 587 - 614 5.1e-06 PPR repeat
PPR_3 PF13812 611 - 667 1.9e-09 Pentatricopeptide repeat domain
PPR_long PF17177 616 - 728 2e-08 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 619 - 712 3.5e-08 Fungal tetratrico peptide repeats
PPR_2 PF13041 623 - 671 2.1e-14 PPR repeat family
PPR_3 PF13812 649 - 704 5e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 653 - 686 2.1e-11 PPR repeat
PPR PF01535 661 - 690 2.4e-06 PPR repeat
PPR_2 PF13041 661 - 706 1.2e-13 PPR repeat family
PPR_1 PF12854 689 - 712 6.5e-08 PPR repeat
PPR_2 PF13041 692 - 741 1.2e-17 PPR repeat family
PPR PF01535 695 - 724 2.1e-06 PPR repeat
PPR_3 PF13812 715 - 772 1.4e-09 Pentatricopeptide repeat domain
PPR_long PF17177 716 - 806 1.9e-09 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 724 - 756 7.6e-11 PPR repeat
PPR PF01535 730 - 760 5.1e-10 PPR repeat
PPR_2 PF13041 732 - 776 5.2e-16 PPR repeat family
PPR_1 PF12854 758 - 790 1.5e-11 PPR repeat
PPR_2 PF13041 762 - 810 1e-13 PPR repeat family
PPR PF01535 765 - 795 7.3e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1881, 1892
Acc36I ACCTGC 1 cut(s) 1192
AccB1I GGYRCC 1 cut(s) 88
AccI GTMKAC 1 cut(s) 1239
AccII CGCG 1 cut(s) 851
AciI CCGC 6 cut(s) 92, 95, 228, 388, 428, 480
AclWI GGATC 2 cut(s) 379, 1568
AcsI RAATTY 6 cut(s) 200, 640, 1135, 1307, 1650, 1867
AcuI CTGAAG 3 cut(s) 488, 1115, 1321
AcyI GRCGYC 1 cut(s) 89
AfaI GTAC 8 cut(s) 158, 342, 538, 1081, 1796, 1906, 1978, 2175
AfiI CCNNNNNNNGG 3 cut(s) 438, 892, 1033
AflIII ACRYGT 3 cut(s) 849, 1973, 2441
AhlI ACTAGT 1 cut(s) 1082
AjiI CACGTC 1 cut(s) 74
Alw21I GWGCWC 1 cut(s) 948
Alw26I GTCTC 1 cut(s) 1940
AlwI GGATC 2 cut(s) 379, 1568
AoxI GGCC 2 cut(s) 1340, 2122
ApeKI GCWGC 5 cut(s) 63, 494, 1445, 1941, 2425
ApoI RAATTY 6 cut(s) 200, 640, 1135, 1307, 1650, 1867
Asp700I GAANNNNTTC 3 cut(s) 16, 1396, 2040
AspLEI GCGC 1 cut(s) 91
AspS9I GGNCC 1 cut(s) 1341
AsuC2I CCSGG 3 cut(s) 122, 713, 2285
AsuHPI GGTGA 4 cut(s) 301, 1792, 1835, 1858
BanI GGYRCC 1 cut(s) 88
BanII GRGCYC 2 cut(s) 1380, 1930
BbsI GAAGAC 2 cut(s) 1833, 2154
Bbv12I GWGCWC 1 cut(s) 948
BbvCI CCTCAGC 1 cut(s) 78
BbvI GCAGC 5 cut(s) 50, 481, 1457, 1928, 2412
BccI CCATC 9 cut(s) 392, 895, 1382, 1630, 1784, 2122, 2204, 2257, 2329
BceAI ACGGC 2 cut(s) 554, 1075
BcgI CGANNNNNNTGC 2 cut(s) 476, 510
BciVI GTATCC 1 cut(s) 136
BclI TGATCA 2 cut(s) 2047, 2362
BcnI CCSGG 3 cut(s) 122, 713, 2285
BcoDI GTCTC 1 cut(s) 1940
BcuI ACTAGT 1 cut(s) 1082
BfaI CTAG 6 cut(s) 1083, 1287, 1515, 2120, 2225, 2384
BfmI CTRYAG 3 cut(s) 1160, 1798, 1859
BfoI RGCGCY 1 cut(s) 92
BfuAI ACCTGC 1 cut(s) 1192
BfuI GTATCC 1 cut(s) 136
BglII AGATCT 1 cut(s) 170
BisI GCNGC 8 cut(s) 64, 92, 95, 228, 495, 1446, 1942, 2426
BlpI GCTNAGC 1 cut(s) 1197
BlsI GCNGC 8 cut(s) 65, 93, 96, 229, 496, 1447, 1943, 2427
BmcAI AGTACT 1 cut(s) 1081
Bme1390I CCNGG 3 cut(s) 122, 713, 2285
BmgBI CACGTC 1 cut(s) 74
BmgT120I GGNCC 1 cut(s) 1341
BmiI GGNNCC 3 cut(s) 90, 801, 1927
BmrFI CCNGG 3 cut(s) 122, 713, 2285
BmrI ACTGGG 1 cut(s) 918
BmsI GCATC 7 cut(s) 196, 763, 1415, 1708, 2020, 2278, 2437
BmuI ACTGGG 1 cut(s) 918
BpiI GAAGAC 2 cut(s) 1833, 2154
BplI GAGNNNNNCTC 2 cut(s) 575, 607
Bpu10I CCTNAGC 1 cut(s) 78
Bpu1102I GCTNAGC 1 cut(s) 1197
BpuEI CTTGAG 2 cut(s) 602, 1508
BpuMI CCSGG 3 cut(s) 122, 713, 2285
BsaHI GRCGYC 1 cut(s) 89
BsaI GGTCTC 1 cut(s) 1940
BsaJI CCNNGG 3 cut(s) 456, 711, 1930
BsaXI ACNNNNNCTCC 6 cut(s) 750, 780, 1424, 1454, 2054, 2084
Bsc4I CCNNNNNNNGG 3 cut(s) 438, 892, 1033
Bse1I ACTGG 3 cut(s) 924, 1224, 1637
Bse3DI GCAATG 2 cut(s) 409, 810
BseDI CCNNGG 3 cut(s) 456, 711, 1930
BseGI GGATG 7 cut(s) 721, 1111, 1641, 1720, 2010, 2293, 2340
BseLI CCNNNNNNNGG 3 cut(s) 438, 892, 1033
BseMI GCAATG 2 cut(s) 409, 810
BseMII CTCAG 5 cut(s) 92, 554, 1188, 1272, 1392
BseNI ACTGG 3 cut(s) 924, 1224, 1637
BseRI GAGGAG 3 cut(s) 229, 890, 1751
BseXI GCAGC 5 cut(s) 50, 481, 1457, 1928, 2412
BseYI CCCAGC 1 cut(s) 1343
BsgI GTGCAG 3 cut(s) 1284, 1762, 2079
Bsh1236I CGCG 1 cut(s) 851
BshFI GGCC 2 cut(s) 1342, 2124
BshNI GGYRCC 1 cut(s) 88
BsiHKAI GWGCWC 1 cut(s) 948
BsiSI CCGG 4 cut(s) 86, 121, 713, 2285
BslFI GGGAC 7 cut(s) 53, 175, 451, 598, 1010, 1643, 2267
BslI CCNNNNNNNGG 3 cut(s) 438, 892, 1033
BsmAI GTCTC 1 cut(s) 1940
BsmFI GGGAC 7 cut(s) 53, 175, 451, 598, 1010, 1643, 2267
BsmI GAATGC 1 cut(s) 1396
BsnI GGCC 2 cut(s) 1342, 2124
Bso31I GGTCTC 1 cut(s) 1940
Bsp1286I GDGCHC 3 cut(s) 948, 1380, 1930
Bsp1407I TGTACA 2 cut(s) 1904, 2173
Bsp143I GATC 5 cut(s) 170, 384, 1560, 2047, 2362
Bsp1720I GCTNAGC 1 cut(s) 1197
Bsp19I CCATGG 1 cut(s) 456
BspACI CCGC 6 cut(s) 92, 95, 228, 388, 428, 480
BspANI GGCC 2 cut(s) 1342, 2124
BspCNI CTCAG 5 cut(s) 91, 553, 1189, 1271, 1393
BspFNI CGCG 1 cut(s) 851
BspHI TCATGA 2 cut(s) 196, 2202
BspLI GGNNCC 3 cut(s) 90, 801, 1927
BspMAI CTGCAG 1 cut(s) 1164
BspMI ACCTGC 1 cut(s) 1192
BspPI GGATC 2 cut(s) 379, 1568
BspT107I GGYRCC 1 cut(s) 88
BspTNI GGTCTC 1 cut(s) 1940
BsrDI GCAATG 2 cut(s) 409, 810
BsrGI TGTACA 2 cut(s) 1904, 2173
BsrI ACTGG 3 cut(s) 924, 1224, 1637
BssECI CCNNGG 3 cut(s) 456, 711, 1930
BssMI GATC 5 cut(s) 170, 384, 1560, 2047, 2362
BssNI GRCGYC 1 cut(s) 89
BssT1I CCWWGG 2 cut(s) 456, 1930
Bst4CI ACNGT 7 cut(s) 536, 680, 1456, 1799, 1823, 1904, 2200
Bst6I CTCTTC 1 cut(s) 218
BstACI GRCGYC 1 cut(s) 89
BstAUI TGTACA 2 cut(s) 1904, 2173
BstC8I GCNNGC 4 cut(s) 369, 411, 1194, 1502
BstDEI CTNAG 7 cut(s) 8, 78, 540, 1197, 1207, 1258, 1401
BstDSI CCRYGG 1 cut(s) 456
BstF5I GGATG 7 cut(s) 721, 1111, 1641, 1720, 2010, 2293, 2340
BstFNI CGCG 1 cut(s) 851
BstH2I RGCGCY 1 cut(s) 92
BstHHI GCGC 1 cut(s) 91
BstKTI GATC 5 cut(s) 173, 387, 1563, 2050, 2365
BstMAI GTCTC 1 cut(s) 1940
BstMBI GATC 5 cut(s) 170, 384, 1560, 2047, 2362
BstMWI GCNNNNNNNGC 6 cut(s) 60, 88, 290, 743, 1198, 2444
BstNSI RCATGY 3 cut(s) 1196, 1504, 2445
BstSCI CCNGG 3 cut(s) 120, 711, 2283
BstSFI CTRYAG 3 cut(s) 1160, 1798, 1859
BstUI CGCG 1 cut(s) 851
BstV1I GCAGC 5 cut(s) 50, 481, 1457, 1928, 2412
BstV2I GAAGAC 2 cut(s) 1833, 2154
BstX2I RGATCY 2 cut(s) 170, 1560
BstYI RGATCY 2 cut(s) 170, 1560
BsuI GTATCC 1 cut(s) 136
BsuRI GGCC 2 cut(s) 1342, 2124
BtgI CCRYGG 1 cut(s) 456
BtrI CACGTC 1 cut(s) 74
BtsCI GGATG 7 cut(s) 721, 1111, 1641, 1720, 2010, 2293, 2340
BtsI GCAGTG 1 cut(s) 2091
BtsIMutI CAGTG 7 cut(s) 432, 1091, 1231, 1301, 1461, 2091, 2196
BveI ACCTGC 1 cut(s) 1192
Cac8I GCNNGC 4 cut(s) 369, 411, 1194, 1502
CciI TCATGA 2 cut(s) 196, 2202
CfoI GCGC 1 cut(s) 91
Cfr13I GGNCC 1 cut(s) 1341
CseI GACGC 2 cut(s) 55, 992
Csp6I GTAC 8 cut(s) 157, 341, 537, 1080, 1795, 1905, 1977, 2174
CviQI GTAC 8 cut(s) 157, 341, 537, 1080, 1795, 1905, 1977, 2174
DdeI CTNAG 7 cut(s) 8, 78, 540, 1197, 1207, 1258, 1401
DinI GGCGCC 1 cut(s) 90
DpnI GATC 5 cut(s) 172, 386, 1562, 2049, 2364
DpnII GATC 5 cut(s) 170, 384, 1560, 2047, 2362
Eam1104I CTCTTC 1 cut(s) 218
EarI CTCTTC 1 cut(s) 218
Eco130I CCWWGG 2 cut(s) 456, 1930
Eco24I GRGCYC 2 cut(s) 1380, 1930
Eco31I GGTCTC 1 cut(s) 1940
Eco57I CTGAAG 3 cut(s) 488, 1115, 1321
EcoRI GAATTC 2 cut(s) 200, 640
EcoT14I CCWWGG 2 cut(s) 456, 1930
EcoT22I ATGCAT 1 cut(s) 1689
EcoT38I GRGCYC 2 cut(s) 1380, 1930
EgeI GGCGCC 1 cut(s) 90
EheI GGCGCC 1 cut(s) 90
ErhI CCWWGG 2 cut(s) 456, 1930
FalI AAGNNNNNCTT 2 cut(s) 1529, 1561
FaqI GGGAC 7 cut(s) 53, 175, 451, 598, 1010, 1643, 2267
FauI CCCGC 1 cut(s) 435
FauNDI CATATG 2 cut(s) 937, 1774
FbaI TGATCA 2 cut(s) 2047, 2362
FblI GTMKAC 1 cut(s) 1239
Fnu4HI GCNGC 8 cut(s) 64, 92, 95, 228, 495, 1446, 1942, 2426
FokI GGATG 7 cut(s) 728, 1118, 1648, 1727, 2017, 2300, 2347
FriOI GRGCYC 2 cut(s) 1380, 1930
Fsp4HI GCNGC 8 cut(s) 64, 92, 95, 228, 495, 1446, 1942, 2426
FspBI CTAG 6 cut(s) 1083, 1287, 1515, 2120, 2225, 2384
GlaI GCGC 1 cut(s) 90
GluI GCNGC 8 cut(s) 64, 92, 95, 228, 495, 1446, 1942, 2426
GsaI CCCAGC 1 cut(s) 1347
HaeII RGCGCY 1 cut(s) 92
HaeIII GGCC 2 cut(s) 1342, 2124
HapII CCGG 4 cut(s) 86, 121, 713, 2285
HgaI GACGC 2 cut(s) 55, 992
HhaI GCGC 1 cut(s) 91
Hin1I GRCGYC 1 cut(s) 89
Hin6I GCGC 1 cut(s) 89
HinP1I GCGC 1 cut(s) 89
HincII GTYRAC 1 cut(s) 250
HindII GTYRAC 1 cut(s) 250
HindIII AAGCTT 3 cut(s) 291, 411, 2346
HinfI GANTC 4 cut(s) 523, 919, 1295, 1490
HpaII CCGG 4 cut(s) 86, 121, 713, 2285
HphI GGTGA 4 cut(s) 301, 1792, 1835, 1858
Hpy166II GTNNAC 9 cut(s) 250, 341, 721, 729, 1240, 1609, 1977, 2222, 2373
Hpy188I TCNGA 2 cut(s) 930, 1402
Hpy188III TCNNGA 5 cut(s) 25, 197, 619, 2045, 2203
Hpy8I GTNNAC 9 cut(s) 250, 341, 721, 729, 1240, 1609, 1977, 2222, 2373
Hpy99I CGWCG 2 cut(s) 227, 986
HpyAV CCTTC 7 cut(s) 6, 757, 994, 1607, 1951, 2051, 2053
HpyCH4III ACNGT 7 cut(s) 536, 680, 1456, 1799, 1823, 1904, 2200
HpyCH4IV ACGT 2 cut(s) 73, 1973
HpyF10VI GCNNNNNNNGC 6 cut(s) 60, 88, 290, 743, 1198, 2444
HpyF3I CTNAG 7 cut(s) 8, 78, 540, 1197, 1207, 1258, 1401
HpySE526I ACGT 2 cut(s) 73, 1973
Hsp92I GRCGYC 1 cut(s) 89
HspAI GCGC 1 cut(s) 89
KasI GGCGCC 1 cut(s) 88
Ksp22I TGATCA 2 cut(s) 2047, 2362
Kzo9I GATC 5 cut(s) 170, 384, 1560, 2047, 2362
LmnI GCTCC 5 cut(s) 4, 799, 820, 951, 1925
Lsp1109I GCAGC 5 cut(s) 50, 481, 1457, 1928, 2412
LweI GCATC 7 cut(s) 196, 763, 1415, 1708, 2020, 2278, 2437
MaeI CTAG 6 cut(s) 1083, 1287, 1515, 2120, 2225, 2384
MaeII ACGT 2 cut(s) 73, 1973
MaeIII GTNAC 5 cut(s) 826, 1126, 1141, 1456, 2293
MalI GATC 5 cut(s) 172, 386, 1562, 2049, 2364
MboI GATC 5 cut(s) 170, 384, 1560, 2047, 2362
MboII GAAGA 8 cut(s) 205, 879, 1838, 1980, 2039, 2154, 2249, 2354
MfeI CAATTG 1 cut(s) 1314
MflI RGATCY 2 cut(s) 170, 1560
MhlI GDGCHC 3 cut(s) 948, 1380, 1930
MluI ACGCGT 1 cut(s) 849
Mly113I GGCGCC 1 cut(s) 89
MmeI TCCRAC 2 cut(s) 492, 948
Mph1103I ATGCAT 1 cut(s) 1689
MroXI GAANNNNTTC 3 cut(s) 16, 1396, 2040
MslI CAYNNNNRTG 2 cut(s) 461, 2201
MspA1I CMGCKG 1 cut(s) 1864
MspI CCGG 4 cut(s) 86, 121, 713, 2285
MspR9I CCNGG 3 cut(s) 122, 713, 2285
MunI CAATTG 1 cut(s) 1314
Mva1269I GAATGC 1 cut(s) 1396
MvnI CGCG 1 cut(s) 851
MwoI GCNNNNNNNGC 6 cut(s) 60, 88, 290, 743, 1198, 2444
NarI GGCGCC 1 cut(s) 89
NciI CCSGG 3 cut(s) 122, 713, 2285
NcoI CCATGG 1 cut(s) 456
NdeI CATATG 2 cut(s) 937, 1774
NdeII GATC 5 cut(s) 170, 384, 1560, 2047, 2362
NlaIV GGNNCC 3 cut(s) 90, 801, 1927
NmuCI GTSAC 2 cut(s) 1456, 2293
NsiI ATGCAT 1 cut(s) 1689
NspI RCATGY 3 cut(s) 1196, 1504, 2445
PaeI GCATGC 2 cut(s) 1196, 1504
PagI TCATGA 2 cut(s) 196, 2202
PciI ACATGT 1 cut(s) 2441
PctI GAATGC 1 cut(s) 1396
PdmI GAANNNNTTC 3 cut(s) 16, 1396, 2040
PfeI GAWTC 4 cut(s) 523, 919, 1295, 1490
PflFI GACNNNGTC 1 cut(s) 1244
PfoI TCCNGGA 1 cut(s) 2283
PkrI GCNGC 8 cut(s) 65, 93, 96, 229, 496, 1447, 1943, 2427
PluTI GGCGCC 1 cut(s) 92
PscI ACATGT 1 cut(s) 2441
PsiI TTATAA 2 cut(s) 1881, 1892
PspFI CCCAGC 1 cut(s) 1343
PspN4I GGNNCC 3 cut(s) 90, 801, 1927
PspPI GGNCC 1 cut(s) 1341
PsrI GAACNNNNNNTAC 4 cut(s) 117, 149, 2130, 2162
PstI CTGCAG 1 cut(s) 1164
PsuI RGATCY 2 cut(s) 170, 1560
PsyI GACNNNGTC 1 cut(s) 1244
PvuII CAGCTG 1 cut(s) 1864
RsaI GTAC 8 cut(s) 158, 342, 538, 1081, 1796, 1906, 1978, 2175
RsaNI GTAC 8 cut(s) 157, 341, 537, 1080, 1795, 1905, 1977, 2174
RseI CAYNNNNRTG 2 cut(s) 461, 2201
SatI GCNGC 8 cut(s) 64, 92, 95, 228, 495, 1446, 1942, 2426
Sau3AI GATC 5 cut(s) 170, 384, 1560, 2047, 2362
Sau96I GGNCC 1 cut(s) 1341
ScaI AGTACT 1 cut(s) 1081
ScrFI CCNGG 3 cut(s) 122, 713, 2285
SduI GDGCHC 3 cut(s) 948, 1380, 1930
SfaNI GCATC 7 cut(s) 196, 763, 1415, 1708, 2020, 2278, 2437
SfcI CTRYAG 3 cut(s) 1160, 1798, 1859
SfoI GGCGCC 1 cut(s) 90
SmiMI CAYNNNNRTG 2 cut(s) 461, 2201
SmlI CTYRAG 2 cut(s) 617, 1523
SmoI CTYRAG 2 cut(s) 617, 1523
SpeI ACTAGT 1 cut(s) 1082
SphI GCATGC 2 cut(s) 1196, 1504
SsiI CCGC 6 cut(s) 92, 95, 228, 388, 428, 480
SspDI GGCGCC 1 cut(s) 88
SspI AATATT 1 cut(s) 2014
SspMI CTAG 6 cut(s) 1083, 1287, 1515, 2120, 2225, 2384
StyD4I CCNGG 3 cut(s) 120, 711, 2283
StyI CCWWGG 2 cut(s) 456, 1930
TaaI ACNGT 7 cut(s) 536, 680, 1456, 1799, 1823, 1904, 2200
TaiI ACGT 2 cut(s) 76, 1976
TaqI TCGA 3 cut(s) 26, 204, 2025
TatI WGTACW 5 cut(s) 340, 536, 1079, 1904, 2173
TauI GCSGC 3 cut(s) 94, 97, 230
TfiI GAWTC 4 cut(s) 523, 919, 1295, 1490
TscAI CASTG 7 cut(s) 439, 1098, 1231, 1308, 1461, 2098, 2203
TseFI GTSAC 2 cut(s) 1456, 2293
TseI GCWGC 5 cut(s) 63, 494, 1445, 1941, 2425
Tsp45I GTSAC 2 cut(s) 1456, 2293
TspDTI ATGAA 9 cut(s) 213, 515, 563, 1122, 1221, 1964, 2093, 2154, 2409
TspGWI ACGGA 3 cut(s) 211, 257, 600
TspRI CASTG 7 cut(s) 439, 1098, 1231, 1308, 1461, 2098, 2203
Tth111I GACNNNGTC 1 cut(s) 1244
XapI RAATTY 6 cut(s) 200, 640, 1135, 1307, 1650, 1867
XceI RCATGY 3 cut(s) 1196, 1504, 2445
XmiI GTMKAC 1 cut(s) 1239
XmnI GAANNNNTTC 3 cut(s) 16, 1396, 2040
XspI CTAG 6 cut(s) 1083, 1287, 1515, 2120, 2225, 2384
ZrmI AGTACT 1 cut(s) 1081
Zsp2I ATGCAT 1 cut(s) 1689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.