MD06G1022200.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0003055
Physical Location & Seq
Reverse (-)
2759277 .. 2765839
6563 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1022200.v1.1.491

Sequence Viewer

Length: 2427 bp
ATGCATTTTTCCATGGATTTGAGAAGGCTTTCAATCCCAAAATCCCATCCTTTTCTCTTCTCCCCCATCAACCGCCCGTGCGTGACCTGCACCGCCCAACACCGCCCCCAAGAACCTCCGCAACCGCCGCCATTGCAGCTTCCGGATCAACCGGAGCCGACCGATCAGAGTTTGCACAACTGGGTATCTTCTATTCTCTCAAAACCATCTTTAGATTCTTCTAAATGCAAGGCTCTCGTACCCCTTTTGTCCCCTCTACATTTTGATCGATTGTTCTGTTCCATTAGATCCAATGTGAACCCCACAACAGCTCTCCATTTCTTCTATTTTGCTTCTGAATCTTTTAAGTTCCGGTTTACTGTTCGATCTTTTTGTGTTTTGGTTCGTCTGCTTATTGGTTCGAATCTTGTGGCCCCTGCGAGATTGCTTTTGATCCGTTCAATTGATGGGAATGTGCCGATTTCATATGCTAACCCCATTCACAGGCATATGGAGATAGCCATTGCCATGTTAGCGTTGAATACCGTGGCTGAACGAGGTGTTGGTGTTCAGGCATTGGACTTGTTGATTCATGTCTACTGCACCCAATTTAAGAAATTTGGTTACGCGGTTGATGTGTTTATGTTTTTTTCTGATAAGGGTGTCTTTCCGTCTTTGAAGACTTGTAATTTTTTGTTGAGTTCGTTAGTGAAGGCCAGCGAACTTCATAAGAGTTATCAAGTATTTGAAGTTATGTCTCGAGGTGTTTCTCCTGACATTTACTTGTTCACTACTGCGATTAATGCTTTTTGTAAGGGAGGGAAGGTTGATGAAGCAATAGGTTTGCTTTCAAAAATGGAAGGGCGGGGGGTTGCTCCAAATGTTGTTACATACAATAATGTTATTCATGGTCTCTGTAAGAGCAGAAGATTGGAGGAGGCCTTCCAGTTTAAGAAGAAGATGGTTGAAAACAATGTGAACCCGAGTCTTATAACATACAGTATACTCATTAATGGTTTGATTAAGCTGGAGAAGTTGTATGAGGCAAATTGTGTTTTGAAGGAAATGTGTAATAGGGGATTTGTCCCAAATGAGGTTGTTTATAACACATTGATTGATGGGTTTTGTAAAAAGGGAAATATTAGTGAGGCACTAAAGATAAAGGATGATATGTTATCCCATGGATTAACTCCCAATTCCGTTACTCTTAATTCTATACTGCAGGGATATTGTAAAACTAATCAGTTGGAGCATGCTGAGCAGATTCTAGACAAGATGCTATCCCATGGTTTATCCATAAATCAAACTGTTTGTTTCTCAGTCATTCACTGGTTATGCATGAAATCTAGGTTCGATTCTGCTCTAAAGTTCACTATAGAAATGCTTTCAAGAAACTGTAGACCCAGCGATGGCTTGCTTACCACGTTGGTTAGTGGGCTTTGTAAAGATGGAAAACATTCCAAGGCAGTTGAACTTTGGTTTAGGCTATGTAACAAAGGATTTGCAGCCAACACACCAACCTCAAATGCTTTAATTCATGGACTTTGTGAATCTGTTAGCATGCAAGAGGTTGTTCCGCGACTCAAACCAATGCTAGAGAGAGGTTTGGTAATGGATAGAATCTCATACAACACACTTATCTCGGGTTGTTGCAAGGAGGGAAAAGTGGATGAAGGGTTTAAGCTTAAGAAAGAGATGGCTAAGCAAGGAATTGAACCAGATACTTATACTTATAATTTGTTAATGCATGGTCTATGTAATATAGGAAAAGTGGACGATGCAGTTAAACTTTGGGATGAGTTTGAAAATCAGGGTCTGGCTCCCAATGTCTATACATATGGGGTGATGATAGATGGGTACTGTAAAGCTGGCAGAGTGGACGAGGGTGAAAAACTTTTCAGTAAGTTGGTAACTAAGAAAGTGGAGCTAAATTCCGTTGTTTACAATACACTAATCAGAGCATACAGCACAAATGGGAATATGACGGCAGCCCTTGGTCTCTGCTTGGACATGAAAAAGAAGGGCATTCAACCAACTTGTGCCACTTATTCTTCCCTTATAGATGGACTGTGCAATATTGGCAGTGTTGATGATGCAAAATGCCTTCTAGATGAAATGAGGAATGAGTGTTTGTTGCCAGGTGTTGTCTGTTATACAGCACTAATTCATGGTTATTGTAAACTAGGACAAATGGATAAAGTAGGGAATATCTTGCTGGAGATGTCTTCATTTAACATTAAACCTAATAAGATTACCTACACTGTCATGATTGATGGGTACTGTAAACTAGGTATTATGGAAGAAGCAACTAGACTTCTATCTGAGATGACAAAGATGGGAGTTGTCCCAGACGCGGTCACCTATAATGCATTAACTAATGGATTTTGTAAGGAAAGGAAGGTGGAAGAAGCTTTTGAAGTGTGTGATCACATGGCCAATAAGGAGTAG

Protein Analysis

809

Amino Acids

90.2

Weight (kDa)

8.48

Isoelectric Point (pI)

32.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 194 - 330 1.5e-08 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 217 - 265 3.1e-06 PPR repeat family
PPR_3 PF13812 243 - 295 6.9e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 247 - 280 3.9e-10 PPR repeat
PPR_2 PF13041 251 - 292 4.5e-11 PPR repeat family
PPR PF01535 255 - 284 3.8e-06 PPR repeat
PPR_3 PF13812 275 - 331 4.4e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 282 - 315 2.1e-11 PPR repeat
PPR_2 PF13041 286 - 335 3.6e-18 PPR repeat family
PPR PF01535 289 - 319 8.3e-07 PPR repeat
PPR_long PF17177 339 - 429 7.3e-07 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 345 - 448 1.7e-08 Fungal tetratrico peptide repeats
PPR_3 PF13812 345 - 403 3.1e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 352 - 384 2.9e-12 PPR repeat
PPR_2 PF13041 356 - 397 4.4e-13 PPR repeat family
PPR PF01535 359 - 389 6.5e-08 PPR repeat
PPR_1 PF12854 388 - 419 4e-08 PPR repeat
PPR_2 PF13041 391 - 428 3.6e-08 PPR repeat family
PPR PF01535 394 - 424 5.3e-06 PPR repeat
PPR_2 PF13041 467 - 509 1.5e-06 PPR repeat family
PPR_1 PF12854 527 - 559 2.5e-11 PPR repeat
PPR_long PF17177 528 - 653 4.4e-08 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 532 - 580 2.4e-19 PPR repeat family
PPR PF01535 534 - 564 1.7e-08 PPR repeat
PPR_3 PF13812 554 - 613 8.9e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 563 - 593 3.1e-11 PPR repeat
PPR_2 PF13041 570 - 615 3.3e-16 PPR repeat family
PPR_1 PF12854 598 - 628 8e-12 PPR repeat
PPR PF01535 604 - 632 2.1e-07 PPR repeat
PPR_3 PF13812 624 - 681 4.7e-11 Pentatricopeptide repeat domain
PPR_long PF17177 625 - 750 2e-08 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 628 - 725 6.8e-07 Fungal tetratrico peptide repeats
PPR_2 PF13041 637 - 685 1.6e-16 PPR repeat family
PPR PF01535 639 - 669 4.5e-06 PPR repeat
PPR_1 PF12854 667 - 700 1.1e-10 PPR repeat
PPR_3 PF13812 695 - 753 1.8e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 706 - 755 1.4e-16 PPR repeat family
PPR PF01535 709 - 739 8.9e-06 PPR repeat
PPR_long PF17177 726 - 806 5.4e-07 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 738 - 770 2.3e-10 PPR repeat
PPR PF01535 744 - 774 3.6e-08 PPR repeat
PPR_2 PF13041 744 - 790 1.1e-15 PPR repeat family
PPR_1 PF12854 772 - 804 2.2e-11 PPR repeat
PPR_2 PF13041 776 - 807 1.7e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 971, 1083, 1713
Acc36I ACCTGC 1 cut(s) 95
AccI GTMKAC 3 cut(s) 576, 982, 1378
AccII CGCG 3 cut(s) 608, 1558, 2333
AccIII TCCGGA 1 cut(s) 142
AclWI GGATC 3 cut(s) 153, 282, 427
AcoI YGGCCR 1 cut(s) 2412
AcsI RAATTY 2 cut(s) 596, 1909
AfaI GTAC 3 cut(s) 240, 1838, 2258
AfiI CCNNNNNNNGG 4 cut(s) 483, 1072, 1388, 2332
AflII CTTAAG 1 cut(s) 1664
AjnI CCWGG 1 cut(s) 2116
AjuI GAANNNNNNNTTGG 2 cut(s) 525, 557
AluBI AGCT 7 cut(s) 139, 311, 1006, 1663, 1847, 1906, 2390
AluI AGCT 7 cut(s) 139, 311, 1006, 1663, 1847, 1906, 2390
Alw26I GTCTC 3 cut(s) 741, 896, 1982
AlwI GGATC 3 cut(s) 153, 282, 427
AlwNI CAGNNNCTG 1 cut(s) 1795
Ama87I CYCGRG 3 cut(s) 738, 961, 1621
Aor13HI TCCGGA 1 cut(s) 142
AoxI GGCC 4 cut(s) 411, 693, 918, 2412
ApeKI GCWGC 3 cut(s) 136, 1484, 1967
ApoI RAATTY 2 cut(s) 596, 1909
AseI ATTAAT 2 cut(s) 780, 990
Asp700I GAANNNNTTC 2 cut(s) 28, 1435
AspS9I GGNCC 1 cut(s) 412
AsuHPI GGTGA 3 cut(s) 1834, 1877, 2329
AsuII TTCGAA 1 cut(s) 401
AvaI CYCGRG 3 cut(s) 738, 961, 1621
BalI TGGCCA 1 cut(s) 2414
BbsI GAAGAC 2 cut(s) 665, 2196
BbvI GCAGC 3 cut(s) 148, 1496, 1979
BceAI ACGGC 1 cut(s) 1980
BcgI CGANNNNNNTGC 2 cut(s) 217, 251
BciT130I CCWGG 1 cut(s) 2118
BclI TGATCA 1 cut(s) 2404
BcoDI GTCTC 3 cut(s) 741, 896, 1982
BfaI CTAG 7 cut(s) 1247, 1326, 1574, 2087, 2162, 2267, 2289
BfmI CTRYAG 3 cut(s) 1199, 1353, 1375
BfrI CTTAAG 1 cut(s) 1664
BfuAI ACCTGC 1 cut(s) 95
BisI GCNGC 4 cut(s) 128, 137, 1485, 1968
BlpI GCTNAGC 2 cut(s) 1236, 1680
BlsI GCNGC 4 cut(s) 129, 138, 1486, 1969
Bme1390I CCNGG 1 cut(s) 2118
BmeT110I CYCGRG 3 cut(s) 738, 961, 1621
BmgT120I GGNCC 1 cut(s) 412
BmiI GGNNCC 3 cut(s) 156, 414, 1800
BmrFI CCNGG 1 cut(s) 2118
BmrI ACTGGG 1 cut(s) 190
BmsI GCATC 3 cut(s) 1245, 1747, 2062
BmuI ACTGGG 1 cut(s) 190
BpiI GAAGAC 2 cut(s) 665, 2196
BpmI CTGGAG 2 cut(s) 1028, 2216
Bpu1102I GCTNAGC 2 cut(s) 1236, 1680
Bpu14I TTCGAA 1 cut(s) 401
Bsa29I ATCGAT 1 cut(s) 268
BsaI GGTCTC 2 cut(s) 896, 1982
BsaJI CCNNGG 6 cut(s) 12, 525, 1159, 1264, 1440, 1972
BsaWI WCCGGW 3 cut(s) 142, 151, 351
BsaXI ACNNNNNCTCC 4 cut(s) 789, 819, 1001, 1031
Bsc4I CCNNNNNNNGG 4 cut(s) 483, 1072, 1388, 2332
Bse1I ACTGG 3 cut(s) 185, 925, 1313
Bse3DI GCAATG 2 cut(s) 131, 501
BseAI TCCGGA 1 cut(s) 142
BseBI CCWGG 1 cut(s) 2118
BseCI ATCGAT 1 cut(s) 268
BseDI CCNNGG 6 cut(s) 12, 525, 1159, 1264, 1440, 1972
BseGI GGATG 4 cut(s) 46, 1150, 1654, 1780
BseLI CCNNNNNNNGG 4 cut(s) 483, 1072, 1388, 2332
BseMI GCAATG 2 cut(s) 131, 501
BseMII CTCAG 3 cut(s) 1227, 1311, 2292
BseNI ACTGG 3 cut(s) 185, 925, 1313
BseRI GAGGAG 1 cut(s) 929
BseXI GCAGC 3 cut(s) 148, 1496, 1979
BseYI CCCAGC 1 cut(s) 1382
BsgI GTGCAG 2 cut(s) 73, 565
Bsh1236I CGCG 3 cut(s) 608, 1558, 2333
Bsh1285I CGRYCG 1 cut(s) 162
BshFI GGCC 4 cut(s) 413, 695, 920, 2414
BshVI ATCGAT 1 cut(s) 268
BsiEI CGRYCG 1 cut(s) 162
BsiHKCI CYCGRG 3 cut(s) 738, 961, 1621
BsiSI CCGG 3 cut(s) 143, 152, 352
BslFI GGGAC 3 cut(s) 235, 1049, 2309
BslI CCNNNNNNNGG 4 cut(s) 483, 1072, 1388, 2332
BsmAI GTCTC 3 cut(s) 741, 896, 1982
BsmFI GGGAC 3 cut(s) 235, 1049, 2309
BsmI GAATGC 1 cut(s) 2004
BsnI GGCC 4 cut(s) 413, 695, 920, 2414
Bso31I GGTCTC 2 cut(s) 896, 1982
BsoBI CYCGRG 3 cut(s) 738, 961, 1621
Bsp119I TTCGAA 1 cut(s) 401
Bsp13I TCCGGA 1 cut(s) 142
Bsp143I GATC 7 cut(s) 145, 163, 265, 287, 365, 432, 2404
Bsp1720I GCTNAGC 2 cut(s) 1236, 1680
Bsp19I CCATGG 3 cut(s) 12, 1159, 1264
BspANI GGCC 4 cut(s) 413, 695, 920, 2414
BspCNI CTCAG 3 cut(s) 1228, 1310, 2293
BspDI ATCGAT 1 cut(s) 268
BspEI TCCGGA 1 cut(s) 142
BspFNI CGCG 3 cut(s) 608, 1558, 2333
BspHI TCATGA 1 cut(s) 2244
BspLI GGNNCC 3 cut(s) 156, 414, 1800
BspMAI CTGCAG 1 cut(s) 1203
BspMI ACCTGC 1 cut(s) 95
BspPI GGATC 3 cut(s) 153, 282, 427
BspT104I TTCGAA 1 cut(s) 401
BspTI CTTAAG 1 cut(s) 1664
BspTNI GGTCTC 2 cut(s) 896, 1982
BsrDI GCAATG 2 cut(s) 131, 501
BsrI ACTGG 3 cut(s) 185, 925, 1313
BssECI CCNNGG 6 cut(s) 12, 525, 1159, 1264, 1440, 1972
BssMI GATC 7 cut(s) 145, 163, 265, 287, 365, 432, 2404
BssNAI GTATAC 1 cut(s) 983
BssT1I CCWWGG 5 cut(s) 12, 1159, 1264, 1440, 1972
Bst1107I GTATAC 1 cut(s) 983
Bst2UI CCWGG 1 cut(s) 2118
Bst4CI ACNGT 9 cut(s) 361, 526, 980, 1288, 1376, 1841, 2049, 2242, 2261
Bst6I CTCTTC 1 cut(s) 62
BstAFI CTTAAG 1 cut(s) 1664
BstBI TTCGAA 1 cut(s) 401
BstC8I GCNNGC 5 cut(s) 697, 1233, 1394, 1541, 1849
BstDEI CTNAG 5 cut(s) 1236, 1297, 1680, 1893, 2301
BstDSI CCRYGG 4 cut(s) 12, 525, 1159, 1264
BstEII GGTNACC 1 cut(s) 2335
BstF5I GGATG 4 cut(s) 46, 1150, 1654, 1780
BstFNI CGCG 3 cut(s) 608, 1558, 2333
BstKTI GATC 7 cut(s) 148, 166, 268, 290, 368, 435, 2407
BstMAI GTCTC 3 cut(s) 741, 896, 1982
BstMBI GATC 7 cut(s) 145, 163, 265, 287, 365, 432, 2404
BstMCI CGRYCG 1 cut(s) 162
BstMWI GCNNNNNNNGC 8 cut(s) 87, 127, 133, 136, 512, 782, 1237, 2058
BstNI CCWGG 1 cut(s) 2118
BstNSI RCATGY 2 cut(s) 1235, 1543
BstPI GGTNACC 1 cut(s) 2335
BstSCI CCNGG 1 cut(s) 2116
BstSFI CTRYAG 3 cut(s) 1199, 1353, 1375
BstUI CGCG 3 cut(s) 608, 1558, 2333
BstV1I GCAGC 3 cut(s) 148, 1496, 1979
BstV2I GAAGAC 2 cut(s) 665, 2196
BstX2I RGATCY 1 cut(s) 287
BstYI RGATCY 1 cut(s) 287
BstZ17I GTATAC 1 cut(s) 983
Bsu15I ATCGAT 1 cut(s) 268
BsuRI GGCC 4 cut(s) 413, 695, 920, 2414
BsuTUI ATCGAT 1 cut(s) 268
BtgI CCRYGG 4 cut(s) 12, 525, 1159, 1264
BtgZI GCGATG 1 cut(s) 1401
BtsCI GGATG 4 cut(s) 46, 1150, 1654, 1780
BtsI GCAGTG 1 cut(s) 2068
BtsIMutI CAGTG 3 cut(s) 1306, 2068, 2238
BveI ACCTGC 1 cut(s) 95
Cac8I GCNNGC 5 cut(s) 697, 1233, 1394, 1541, 1849
CaiI CAGNNNCTG 1 cut(s) 1795
CciI TCATGA 1 cut(s) 2244
Cfr13I GGNCC 1 cut(s) 412
ClaI ATCGAT 1 cut(s) 268
CseI GACGC 1 cut(s) 2339
Csp6I GTAC 3 cut(s) 239, 1837, 2257
CviQI GTAC 3 cut(s) 239, 1837, 2257
DdeI CTNAG 5 cut(s) 1236, 1297, 1680, 1893, 2301
DpnI GATC 7 cut(s) 147, 165, 267, 289, 367, 434, 2406
DpnII GATC 7 cut(s) 145, 163, 265, 287, 365, 432, 2404
EaeI YGGCCR 1 cut(s) 2412
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
Eco130I CCWWGG 5 cut(s) 12, 1159, 1264, 1440, 1972
Eco147I AGGCCT 1 cut(s) 920
Eco31I GGTCTC 2 cut(s) 896, 1982
Eco88I CYCGRG 3 cut(s) 738, 961, 1621
Eco91I GGTNACC 1 cut(s) 2335
EcoO65I GGTNACC 1 cut(s) 2335
EcoRII CCWGG 1 cut(s) 2116
EcoT14I CCWWGG 5 cut(s) 12, 1159, 1264, 1440, 1972
EcoT22I ATGCAT 4 cut(s) 6, 1319, 1728, 2350
ErhI CCWWGG 5 cut(s) 12, 1159, 1264, 1440, 1972
FalI AAGNNNNNCTT 2 cut(s) 629, 661
FaqI GGGAC 3 cut(s) 235, 1049, 2309
FauI CCCGC 1 cut(s) 837
FauNDI CATATG 3 cut(s) 466, 489, 1816
FbaI TGATCA 1 cut(s) 2404
FblI GTMKAC 3 cut(s) 576, 982, 1378
Fnu4HI GCNGC 4 cut(s) 128, 137, 1485, 1968
FokI GGATG 4 cut(s) 33, 1157, 1661, 1787
Fsp4HI GCNGC 4 cut(s) 128, 137, 1485, 1968
FspBI CTAG 7 cut(s) 1247, 1326, 1574, 2087, 2162, 2267, 2289
GluI GCNGC 4 cut(s) 128, 137, 1485, 1968
GsaI CCCAGC 1 cut(s) 1386
GsuI CTGGAG 2 cut(s) 1028, 2216
HaeIII GGCC 4 cut(s) 413, 695, 920, 2414
HapII CCGG 3 cut(s) 143, 152, 352
HgaI GACGC 1 cut(s) 2339
HindIII AAGCTT 2 cut(s) 1661, 2388
HpaII CCGG 3 cut(s) 143, 152, 352
HphI GGTGA 3 cut(s) 1834, 1877, 2329
Hpy188I TCNGA 5 cut(s) 168, 337, 634, 1937, 2302
Hpy188III TCNNGA 7 cut(s) 143, 738, 752, 1247, 1368, 2087, 2245
HpyCH4III ACNGT 9 cut(s) 361, 526, 980, 1288, 1376, 1841, 2049, 2242, 2261
HpyCH4IV ACGT 1 cut(s) 1403
HpyF10VI GCNNNNNNNGC 8 cut(s) 87, 127, 133, 136, 512, 782, 1237, 2058
HpyF3I CTNAG 5 cut(s) 1236, 1297, 1680, 1893, 2301
HpySE526I ACGT 1 cut(s) 1403
Kpn2I TCCGGA 1 cut(s) 142
Ksp22I TGATCA 1 cut(s) 2404
Kzo9I GATC 7 cut(s) 145, 163, 265, 287, 365, 432, 2404
LmnI GCTCC 5 cut(s) 154, 859, 1228, 1804, 1903
Lsp1109I GCAGC 3 cut(s) 148, 1496, 1979
LweI GCATC 3 cut(s) 1245, 1747, 2062
MaeI CTAG 7 cut(s) 1247, 1326, 1574, 2087, 2162, 2267, 2289
MaeII ACGT 1 cut(s) 1403
MaeIII GTNAC 7 cut(s) 82, 602, 865, 1180, 1469, 1888, 2335
MalI GATC 7 cut(s) 147, 165, 267, 289, 367, 434, 2406
MboI GATC 7 cut(s) 145, 163, 265, 287, 365, 432, 2404
MfeI CAATTG 1 cut(s) 441
MflI RGATCY 1 cut(s) 287
MlsI TGGCCA 1 cut(s) 2414
MluNI TGGCCA 1 cut(s) 2414
MlyI GAGTC 2 cut(s) 973, 1554
MmeI TCCRAC 1 cut(s) 1206
Mox20I TGGCCA 1 cut(s) 2414
Mph1103I ATGCAT 4 cut(s) 6, 1319, 1728, 2350
MroI TCCGGA 1 cut(s) 142
MroXI GAANNNNTTC 2 cut(s) 28, 1435
MscI TGGCCA 1 cut(s) 2414
MslI CAYNNNNRTG 2 cut(s) 506, 2243
Msp20I TGGCCA 1 cut(s) 2414
MspCI CTTAAG 1 cut(s) 1664
MspI CCGG 3 cut(s) 143, 152, 352
MspR9I CCNGG 1 cut(s) 2118
MunI CAATTG 1 cut(s) 441
Mva1269I GAATGC 1 cut(s) 2004
MvaI CCWGG 1 cut(s) 2118
MvnI CGCG 3 cut(s) 608, 1558, 2333
MwoI GCNNNNNNNGC 8 cut(s) 87, 127, 133, 136, 512, 782, 1237, 2058
NcoI CCATGG 3 cut(s) 12, 1159, 1264
NdeI CATATG 3 cut(s) 466, 489, 1816
NdeII GATC 7 cut(s) 145, 163, 265, 287, 365, 432, 2404
NlaIV GGNNCC 3 cut(s) 156, 414, 1800
NmuCI GTSAC 2 cut(s) 82, 2335
NsiI ATGCAT 4 cut(s) 6, 1319, 1728, 2350
NspI RCATGY 2 cut(s) 1235, 1543
NspV TTCGAA 1 cut(s) 401
PaeI GCATGC 2 cut(s) 1235, 1543
PaeR7I CTCGAG 1 cut(s) 738
PagI TCATGA 1 cut(s) 2244
PceI AGGCCT 1 cut(s) 920
PctI GAATGC 1 cut(s) 2004
PdmI GAANNNNTTC 2 cut(s) 28, 1435
PfeI GAWTC 8 cut(s) 215, 338, 403, 568, 1243, 1334, 1529, 1599
PflFI GACNNNGTC 1 cut(s) 2333
PkrI GCNGC 4 cut(s) 129, 138, 1486, 1969
PleI GAGTC 2 cut(s) 972, 1554
PpsI GAGTC 2 cut(s) 972, 1554
PshBI ATTAAT 2 cut(s) 780, 990
PsiI TTATAA 3 cut(s) 971, 1083, 1713
Psp6I CCWGG 1 cut(s) 2116
PspEI GGTNACC 1 cut(s) 2335
PspFI CCCAGC 1 cut(s) 1382
PspGI CCWGG 1 cut(s) 2116
PspN4I GGNNCC 3 cut(s) 156, 414, 1800
PspPI GGNCC 1 cut(s) 412
PstI CTGCAG 1 cut(s) 1203
PstNI CAGNNNCTG 1 cut(s) 1795
PsuI RGATCY 1 cut(s) 287
PsyI GACNNNGTC 1 cut(s) 2333
RsaI GTAC 3 cut(s) 240, 1838, 2258
RsaNI GTAC 3 cut(s) 239, 1837, 2257
RseI CAYNNNNRTG 2 cut(s) 506, 2243
SatI GCNGC 4 cut(s) 128, 137, 1485, 1968
Sau3AI GATC 7 cut(s) 145, 163, 265, 287, 365, 432, 2404
Sau96I GGNCC 1 cut(s) 412
SchI GAGTC 2 cut(s) 973, 1554
ScrFI CCNGG 1 cut(s) 2118
SfaNI GCATC 3 cut(s) 1245, 1747, 2062
SfcI CTRYAG 3 cut(s) 1199, 1353, 1375
Sfr274I CTCGAG 1 cut(s) 738
SfuI TTCGAA 1 cut(s) 401
SlaI CTCGAG 1 cut(s) 738
SmiMI CAYNNNNRTG 2 cut(s) 506, 2243
SmlI CTYRAG 2 cut(s) 738, 1664
SmoI CTYRAG 2 cut(s) 738, 1664
SphI GCATGC 2 cut(s) 1235, 1543
SseBI AGGCCT 1 cut(s) 920
SspI AATATT 2 cut(s) 1120, 2056
SspMI CTAG 7 cut(s) 1247, 1326, 1574, 2087, 2162, 2267, 2289
StuI AGGCCT 1 cut(s) 920
StyD4I CCNGG 1 cut(s) 2116
StyI CCWWGG 5 cut(s) 12, 1159, 1264, 1440, 1972
TaaI ACNGT 9 cut(s) 361, 526, 980, 1288, 1376, 1841, 2049, 2242, 2261
TaiI ACGT 1 cut(s) 1406
TaqI TCGA 5 cut(s) 268, 364, 401, 739, 1332
TaqII GACCGA 1 cut(s) 176
TauI GCSGC 1 cut(s) 130
TfiI GAWTC 8 cut(s) 215, 338, 403, 568, 1243, 1334, 1529, 1599
TscAI CASTG 3 cut(s) 1313, 2068, 2245
TseFI GTSAC 2 cut(s) 82, 2335
TseI GCWGC 3 cut(s) 136, 1484, 1967
Tsp45I GTSAC 2 cut(s) 82, 2335
TspGWI ACGGA 4 cut(s) 425, 639, 1168, 1903
TspRI CASTG 3 cut(s) 1313, 2068, 2245
Tth111I GACNNNGTC 1 cut(s) 2333
Vha464I CTTAAG 1 cut(s) 1664
VspI ATTAAT 2 cut(s) 780, 990
XapI RAATTY 2 cut(s) 596, 1909
XbaI TCTAGA 2 cut(s) 1246, 2086
XceI RCATGY 2 cut(s) 1235, 1543
XhoI CTCGAG 1 cut(s) 738
XmiI GTMKAC 3 cut(s) 576, 982, 1378
XmnI GAANNNNTTC 2 cut(s) 28, 1435
XspI CTAG 7 cut(s) 1247, 1326, 1574, 2087, 2162, 2267, 2289
Zsp2I ATGCAT 4 cut(s) 6, 1319, 1728, 2350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.