Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7D

Orthogroup ID

OG0003055

Physical Location & Seq

Genomic Coordinates Reverse (-)

32180384 .. 32182069

Gene Structure 1686 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7DG287900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1686 bp

ATGCAATTCTCAATGGATTTGAGAAGGCTTCCGATCTCCAAACCCGCTTCTCTCTTCTACCCAATCACACGCCCCTTAACGGGCGTGACATTCACCTTCCAACGCCGCCACGACCCTCCCCAGCCGCCGCCGTTACAGCCTCCGAGTCCACCACGGCCTCCCAATCCGGGTTTGCTCAACTGGGTATCTTCTATTCTCTCAAACCCATCTTTGGATTCTTCTAAATGTGAATCTCTCATGCCCCTTTTGTCCCCTCATGAATTTGATCAGTTATTCTACTCCATTAGGTCCAATGTGAACCCCAAAACAGCTCTTCATTTCTTTCATTTTGCTTCTCAATCTTTTAAGTTTCGGTTTACTGTTCGATCTTTCTGTGTTTTGGTTCATTTGCTCATTGCTTCAAATCTTGGGCCTCCTGCGAGGTTGCTTTTGATTCGTTTGATTGATGGGAATGTCCCAGTTTTGTGTGCTGACCCCAACAGCAAGCATATTGAGATAGCCGTCGCAATGTCGGAGTTGAACAATGCGTCCCAACCGGCTCTGGGGATTCAGGCATTGGATATGTTAATTCATGTTTACTGTACCCAGTTCAAGAATTTGGGTTTTGGTTATGCTGTTCATGTGTTTGAGCATTTTTCGGATAAGGGTGTGTTTCCGTCTTTGAAGACTTGTAATGTTTTGCTGAGTTCTTTAGTGAAGGCTAACGCTCTTGAGAAGAGCTGTCATGTATTTGAAGTTATGACTCGAGGTGTTTGTCCTGATGTTTTCTTGTTTACTACTGCAATTAATGCGTTTTGCAAGGGAGGGAAGGTGGATGATGCAATAGCTGTGCTCTCAAAAATGGAGAGCATTGGTGTTGCTCCAAATGTGGTTACTTACAATAGCGTTATCCACGGGCTATGTAAGAGTAGAAGATTAGAGGAGGCTTTCCAGTTTAAGGAGAAGATGGTAAAGAATAATGTGAAGCCGAGTCTTATAACATACAGTGTGCTCATTAATGGTTTGATTAAGCTGGAGAAGTTTTATGAGGCAAATTGTGTTTTCAAGGAAATGCGTAGTTGGGGGTTTGTCCCGAATGAAGTTGTATATAACACTCTCATTAATGGGTATTGTACTATGGGAAATATTGGCGAGGCACTCGAGATAAGGGATGATATGGTATCGAATGGGGTTACCCCTAATTCTGTTACTCTGAATTCGCTACTGCATGGATTTTGTAAAAGTAATCAATTTGAGCATGCTGAGCAATTTTTGGACAAGATGCTAGCCAGTGGTTTACCAGTCAACCAAGTTGTTTCTTACTCAGTCATTGACTGGTTATGCATGAAATCTAGGTTTGATTCTGCACTAAGATTCACTACCGAAATGTTATTAAGAAACTTTAGGCCCAGTGATAGTTTGCTTACTACATTGTTTGTTGGGCTATGCAAAGATGGGAAGAATTTAGAGGCAATCGAACTTTGGTTTAGGCTATCGGAGAAGGGGTTTGCAGCCAACACAGCGACCACAAATGCTCTAATCCATGGACTTTGTGAATCTGGTAGCATCGAAGAGGTTGTTAGGCTACTCAAGGCAATGCTTGAGAGGGGTTTGGCATTGGATAAGTTCTCATACAACACACTCATCTTAAGTTGTTGCAAGGAGGGAAGAGTGGCAGAAGGTTTTAAGCTTAGAGAAGATGTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

561

Amino Acids

62.61

Weight (kDa)

8.47

Isoelectric Point (pI)

42.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_long	PF17177	204 - 332	2.6e-09	Pentacotripeptide-repeat region of PRORP
TPR_24	PF23276	243 - 350	2e-06	Fungal tetratrico peptide repeats
PPR_3	PF13812	245 - 298	8.9e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	249 - 282	2.2e-11	PPR repeat
PPR_2	PF13041	253 - 302	2.4e-15	PPR repeat family
PPR_1	PF12854	285 - 317	1.2e-12	PPR repeat
PPR_2	PF13041	288 - 337	6.2e-19	PPR repeat family
PPR_3	PF13812	289 - 333	1.3e-07	Pentatricopeptide repeat domain
PPR	PF01535	291 - 321	1.8e-07	PPR repeat
PPR_3	PF13812	313 - 370	2.8e-07	Pentatricopeptide repeat domain
TPR_24	PF23276	347 - 430	3.9e-06	Fungal tetratrico peptide repeats
PPR_3	PF13812	347 - 403	7.8e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	355 - 387	4.5e-11	PPR repeat
PPR_2	PF13041	358 - 407	1.6e-17	PPR repeat family
PPR	PF01535	361 - 391	9e-08	PPR repeat
PPR_1	PF12854	389 - 421	1.7e-09	PPR repeat
PPR_2	PF13041	393 - 431	2.4e-08	PPR repeat family
PPR	PF01535	396 - 426	6.3e-06	PPR repeat
PPR_2	PF13041	469 - 511	5.5e-06	PPR repeat family
PPR_2	PF13041	499 - 547	5.1e-12	PPR repeat family
PPR_1	PF12854	529 - 559	1.2e-06	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003055)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G19440 AT4G19440 AT4G19440 AT4G19440
fragaria_vesca	FvH4_4g10410 FvH4_4g10410 FvH4_4g10440 FvH4_5g24430 FvH4_5g24430 FvH4_5g24430 FvH4_5g24430
malus_domestica	MD06G1022200.v1.1
prunus_persica	Prupe.3G018200_v2.0.a1
pyrus_communis	pycom06g01610
rosa_chinensis	RchiOBHm_Chr4g0405931 RchiOBHm_Chr7g0214821 RchiOBHm_Chr7g0219861
rosa_laevigata	RLG00000002716 RLG00000008810
rosa_multiflora	Rmu_sc0002308.1_g000021 Rmu_sc0019702.1_g000004 Rmu_ssc0000087.1_g000071
rosa_roxburghii	Rroxscaffold_3G00244980 Rroxscaffold_5G00350670
rosa_rugosa	Rorug04G0064100 Rorug07G0147600 Rorug07G0147700 Rorug07G0147800
rosa_samantha	Rh4AG134900 Rh4BG130500 Rh4CG142300 Rh4DG129200 Rh7AG284500 Rh7BG275300 Rh7CG303700 Rh7DG287900
rosa_wichuraiana	Rw4G010980 Rw7G024260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	977
AciI	CCGC	4 cut(s)	45, 106, 125, 128
AcsI	RAATTY	4 cut(s)	260, 595, 1195, 1441
AfaI	GTAC	2 cut(s)	583, 1114
AfiI	CCNNNNNNNGG	6 cut(s)	79, 80, 167, 211, 542, 937
AflII	CTTAAG	1 cut(s)	1627
AgsI	TTSAA	6 cut(s)	402, 520, 592, 664, 734, 1045
AloI	GAACNNNNNNTCC	2 cut(s)	512, 544
AluBI	AGCT	5 cut(s)	311, 720, 827, 1012, 1669
AluI	AGCT	5 cut(s)	311, 720, 827, 1012, 1669
Alw21I	GWGCWC	2 cut(s)	834, 993
Ama87I	CYCGRG	2 cut(s)	744, 1139
AoxI	GGCC	3 cut(s)	155, 410, 1385
ApeKI	GCWGC	1 cut(s)	1490
ApoI	RAATTY	4 cut(s)	260, 595, 1195, 1441
AseI	ATTAAT	3 cut(s)	786, 996, 1101
AspS9I	GGNCC	3 cut(s)	288, 410, 1386
AsuC2I	CCSGG	1 cut(s)	168
AsuHPI	GGTGA	1 cut(s)	85
AsuNHI	GCTAGC	1 cut(s)	1264
AvaI	CYCGRG	2 cut(s)	744, 1139
AvaII	GGWCC	1 cut(s)	288
BbsI	GAAGAC	1 cut(s)	671
Bbv12I	GWGCWC	2 cut(s)	834, 993
BbvI	GCAGC	1 cut(s)	1502
BccI	CCATC	4 cut(s)	214, 440, 940, 1427
BceAI	ACGGC	3 cut(s)	115, 170, 485
BclI	TGATCA	1 cut(s)	265
BcnI	CCSGG	1 cut(s)	168
BfaI	CTAG	2 cut(s)	1265, 1332
BfrI	CTTAAG	1 cut(s)	1627
BisI	GCNGC	4 cut(s)	106, 125, 128, 1491
BlpI	GCTNAGC	1 cut(s)	1242
BlsI	GCNGC	4 cut(s)	107, 126, 129, 1492
Bme1390I	CCNGG	1 cut(s)	168
Bme18I	GGWCC	1 cut(s)	288
BmeT110I	CYCGRG	2 cut(s)	744, 1139
BmgT120I	GGNCC	3 cut(s)	288, 410, 1386
BmrFI	CCNGG	1 cut(s)	168
BmrI	ACTGGG	4 cut(s)	190, 452, 580, 1383
BmsI	GCATC	3 cut(s)	808, 1251, 1554
BmtI	GCTAGC	1 cut(s)	1268
BmuI	ACTGGG	4 cut(s)	190, 452, 580, 1383
BpiI	GAAGAC	1 cut(s)	671
BpmI	CTGGAG	1 cut(s)	1034
Bpu1102I	GCTNAGC	1 cut(s)	1242
BpuEI	CTTGAG	3 cut(s)	731, 1553, 1601
BpuMI	CCSGG	1 cut(s)	168
BsaJI	CCNNGG	3 cut(s)	152, 892, 1522
BsaXI	ACNNNNNCTCC	4 cut(s)	795, 825, 1469, 1499
Bsc4I	CCNNNNNNNGG	6 cut(s)	79, 80, 167, 211, 542, 937
Bse118I	RCCGGY	1 cut(s)	535
Bse1I	ACTGG	8 cut(s)	185, 458, 586, 931, 1269, 1280, 1319, 1389
Bse3DI	GCAATG	3 cut(s)	393, 513, 1581
BseDI	CCNNGG	3 cut(s)	152, 892, 1522
BseGI	GGATG	2 cut(s)	820, 1156
BseLI	CCNNNNNNNGG	6 cut(s)	79, 80, 167, 211, 542, 937
BseMI	GCAATG	3 cut(s)	393, 513, 1581
BseMII	CTCAG	3 cut(s)	674, 1233, 1317
BseNI	ACTGG	8 cut(s)	185, 458, 586, 931, 1269, 1280, 1319, 1389
BseRI	GAGGAG	1 cut(s)	935
BseXI	GCAGC	1 cut(s)	1502
BseYI	CCCAGC	1 cut(s)	120
BsgI	GTGCAG	1 cut(s)	1329
BshFI	GGCC	3 cut(s)	157, 412, 1387
BsiHKAI	GWGCWC	2 cut(s)	834, 993
BsiHKCI	CYCGRG	2 cut(s)	744, 1139
BsiSI	CCGG	2 cut(s)	167, 536
BslFI	GGGAC	4 cut(s)	235, 440, 514, 1055
BslI	CCNNNNNNNGG	6 cut(s)	79, 80, 167, 211, 542, 937
BsmFI	GGGAC	4 cut(s)	235, 440, 514, 1055
BsnI	GGCC	3 cut(s)	157, 412, 1387
BsoBI	CYCGRG	2 cut(s)	744, 1139
Bsp1286I	GDGCHC	2 cut(s)	834, 993
Bsp143I	GATC	3 cut(s)	33, 265, 365
Bsp1720I	GCTNAGC	1 cut(s)	1242
Bsp19I	CCATGG	1 cut(s)	1522
BspACI	CCGC	4 cut(s)	45, 106, 125, 128
BspANI	GGCC	3 cut(s)	157, 412, 1387
BspCNI	CTCAG	3 cut(s)	675, 1234, 1316
BspHI	TCATGA	1 cut(s)	256
BspOI	GCTAGC	1 cut(s)	1268
BspQI	GCTCTTC	2 cut(s)	318, 710
BspTI	CTTAAG	1 cut(s)	1627
BsrDI	GCAATG	3 cut(s)	393, 513, 1581
BsrFI	RCCGGY	1 cut(s)	535
BsrI	ACTGG	8 cut(s)	185, 458, 586, 931, 1269, 1280, 1319, 1389
BssAI	RCCGGY	1 cut(s)	535
BssECI	CCNNGG	3 cut(s)	152, 892, 1522
BssMI	GATC	3 cut(s)	33, 265, 365
BssT1I	CCWWGG	1 cut(s)	1522
Bst4CI	ACNGT	3 cut(s)	361, 581, 986
Bst6I	CTCTTC	5 cut(s)	59, 318, 710, 1545, 1642
BstAFI	CTTAAG	1 cut(s)	1627
BstAPI	GCANNNNNTGC	1 cut(s)	788
BstC8I	GCNNGC	3 cut(s)	485, 1239, 1266
BstDEI	CTNAG	5 cut(s)	683, 1242, 1303, 1349, 1670
BstDSI	CCRYGG	3 cut(s)	152, 892, 1522
BstEII	GGTNACC	1 cut(s)	1171
BstF5I	GGATG	2 cut(s)	820, 1156
BstKTI	GATC	3 cut(s)	36, 268, 368
BstMBI	GATC	3 cut(s)	33, 265, 365
BstMWI	GCNNNNNNNGC	4 cut(s)	136, 788, 1243, 1499
BstNSI	RCATGY	1 cut(s)	1241
BstPI	GGTNACC	1 cut(s)	1171
BstSCI	CCNGG	1 cut(s)	166
BstV1I	GCAGC	1 cut(s)	1502
BstV2I	GAAGAC	1 cut(s)	671
BsuRI	GGCC	3 cut(s)	157, 412, 1387
BtgI	CCRYGG	3 cut(s)	152, 892, 1522
BtsCI	GGATG	2 cut(s)	820, 1156
BtsIMutI	CAGTG	3 cut(s)	991, 1276, 1396
Cac8I	GCNNGC	3 cut(s)	485, 1239, 1266
CciI	TCATGA	1 cut(s)	256
Cfr10I	RCCGGY	1 cut(s)	535
Cfr13I	GGNCC	3 cut(s)	288, 410, 1386
CseI	GACGC	1 cut(s)	516
Csp6I	GTAC	2 cut(s)	582, 1113
CviAII	CATG	9 cut(s)	238, 257, 572, 620, 725, 1208, 1238, 1324, 1523
CviQI	GTAC	2 cut(s)	582, 1113
DdeI	CTNAG	5 cut(s)	683, 1242, 1303, 1349, 1670
DpnI	GATC	3 cut(s)	35, 267, 367
DpnII	GATC	3 cut(s)	33, 265, 365
Eam1104I	CTCTTC	5 cut(s)	59, 318, 710, 1545, 1642
EarI	CTCTTC	5 cut(s)	59, 318, 710, 1545, 1642
Eco130I	CCWWGG	1 cut(s)	1522
Eco47I	GGWCC	1 cut(s)	288
Eco88I	CYCGRG	2 cut(s)	744, 1139
Eco91I	GGTNACC	1 cut(s)	1171
EcoO65I	GGTNACC	1 cut(s)	1171
EcoRI	GAATTC	1 cut(s)	1195
EcoT14I	CCWWGG	1 cut(s)	1522
EcoT22I	ATGCAT	1 cut(s)	1325
ErhI	CCWWGG	1 cut(s)	1522
FaeI	CATG	9 cut(s)	241, 260, 575, 623, 728, 1211, 1241, 1327, 1526
FaqI	GGGAC	4 cut(s)	235, 440, 514, 1055
FatI	CATG	9 cut(s)	237, 256, 571, 619, 724, 1207, 1237, 1323, 1522
FauI	CCCGC	1 cut(s)	52
FbaI	TGATCA	1 cut(s)	265
Fnu4HI	GCNGC	4 cut(s)	106, 125, 128, 1491
FokI	GGATG	2 cut(s)	827, 1163
Fsp4HI	GCNGC	4 cut(s)	106, 125, 128, 1491
FspBI	CTAG	2 cut(s)	1265, 1332
GluI	GCNGC	4 cut(s)	106, 125, 128, 1491
GsaI	CCCAGC	1 cut(s)	124
GsuI	CTGGAG	1 cut(s)	1034
HaeIII	GGCC	3 cut(s)	157, 412, 1387
HapII	CCGG	2 cut(s)	167, 536
HgaI	GACGC	1 cut(s)	516
Hin1II	CATG	9 cut(s)	241, 260, 575, 623, 728, 1211, 1241, 1327, 1526
HincII	GTYRAC	1 cut(s)	1285
HindII	GTYRAC	1 cut(s)	1285
HindIII	AAGCTT	1 cut(s)	1667
HpaII	CCGG	2 cut(s)	167, 536
HphI	GGTGA	1 cut(s)	85
Hpy166II	GTNNAC	7 cut(s)	149, 298, 357, 577, 774, 1277, 1285
Hpy188I	TCNGA	6 cut(s)	33, 144, 514, 640, 1194, 1477
Hpy188III	TCNNGA	6 cut(s)	257, 592, 710, 758, 1072, 1141
Hpy8I	GTNNAC	7 cut(s)	149, 298, 357, 577, 774, 1277, 1285
Hpy99I	CGWCG	1 cut(s)	506
HpyAV	CCTTC	6 cut(s)	18, 106, 691, 802, 1474, 1652
HpyCH4III	ACNGT	3 cut(s)	361, 581, 986
HpyF10VI	GCNNNNNNNGC	4 cut(s)	136, 788, 1243, 1499
HpyF3I	CTNAG	5 cut(s)	683, 1242, 1303, 1349, 1670
Hsp92II	CATG	9 cut(s)	241, 260, 575, 623, 728, 1211, 1241, 1327, 1526
Ksp22I	TGATCA	1 cut(s)	265
Kzo9I	GATC	3 cut(s)	33, 265, 365
LguI	GCTCTTC	2 cut(s)	318, 710
LmnI	GCTCC	1 cut(s)	865
Lsp1109I	GCAGC	1 cut(s)	1502
LweI	GCATC	3 cut(s)	808, 1251, 1554
MaeI	CTAG	2 cut(s)	1265, 1332
MaeIII	GTNAC	5 cut(s)	85, 132, 871, 1171, 1186
MalI	GATC	3 cut(s)	35, 267, 367
MboI	GATC	3 cut(s)	33, 265, 365
MhlI	GDGCHC	2 cut(s)	834, 993
MlyI	GAGTC	3 cut(s)	154, 736, 979
MmeI	TCCRAC	2 cut(s)	124, 492
Mph1103I	ATGCAT	1 cut(s)	1325
MspCI	CTTAAG	1 cut(s)	1627
MspI	CCGG	2 cut(s)	167, 536
MspR9I	CCNGG	1 cut(s)	168
MwoI	GCNNNNNNNGC	4 cut(s)	136, 788, 1243, 1499
NciI	CCSGG	1 cut(s)	168
NcoI	CCATGG	1 cut(s)	1522
NdeII	GATC	3 cut(s)	33, 265, 365
NheI	GCTAGC	1 cut(s)	1264
NlaIII	CATG	9 cut(s)	241, 260, 575, 623, 728, 1211, 1241, 1327, 1526
NmeAIII	GCCGAG	1 cut(s)	993
NmuCI	GTSAC	1 cut(s)	85
NsiI	ATGCAT	1 cut(s)	1325
NspI	RCATGY	1 cut(s)	1241
PaeI	GCATGC	1 cut(s)	1241
PaeR7I	CTCGAG	2 cut(s)	744, 1139
PagI	TCATGA	1 cut(s)	256
PciSI	GCTCTTC	2 cut(s)	318, 710
PfeI	GAWTC	7 cut(s)	215, 230, 433, 547, 1340, 1353, 1535
PkrI	GCNGC	4 cut(s)	107, 126, 129, 1492
PleI	GAGTC	3 cut(s)	153, 736, 978
PpsI	GAGTC	3 cut(s)	153, 736, 978
PshBI	ATTAAT	3 cut(s)	786, 996, 1101
PsiI	TTATAA	1 cut(s)	977
PspEI	GGTNACC	1 cut(s)	1171
PspFI	CCCAGC	1 cut(s)	120
PspPI	GGNCC	3 cut(s)	288, 410, 1386
PspXI	VCTCGAGB	1 cut(s)	744
RsaI	GTAC	2 cut(s)	583, 1114
RsaNI	GTAC	2 cut(s)	582, 1113
SapI	GCTCTTC	2 cut(s)	318, 710
SatI	GCNGC	4 cut(s)	106, 125, 128, 1491
Sau3AI	GATC	3 cut(s)	33, 265, 365
Sau96I	GGNCC	3 cut(s)	288, 410, 1386
SchI	GAGTC	3 cut(s)	154, 736, 979
ScrFI	CCNGG	1 cut(s)	168
SduI	GDGCHC	2 cut(s)	834, 993
SfaNI	GCATC	3 cut(s)	808, 1251, 1554
Sfr274I	CTCGAG	2 cut(s)	744, 1139
SinI	GGWCC	1 cut(s)	288
SlaI	CTCGAG	2 cut(s)	744, 1139
SmlI	CTYRAG	6 cut(s)	710, 744, 1139, 1568, 1580, 1627
SmoI	CTYRAG	6 cut(s)	710, 744, 1139, 1568, 1580, 1627
SphI	GCATGC	1 cut(s)	1241
SsiI	CCGC	4 cut(s)	45, 106, 125, 128
SspI	AATATT	1 cut(s)	1126
SspMI	CTAG	2 cut(s)	1265, 1332
StyD4I	CCNGG	1 cut(s)	166
StyI	CCWWGG	1 cut(s)	1522
TaaI	ACNGT	3 cut(s)	361, 581, 986
TaqI	TCGA	6 cut(s)	364, 745, 1140, 1163, 1455, 1548
TatI	WGTACW	1 cut(s)	1112
TauI	GCSGC	3 cut(s)	108, 127, 130
TfiI	GAWTC	7 cut(s)	215, 230, 433, 547, 1340, 1353, 1535
TscAI	CASTG	3 cut(s)	991, 1276, 1396
TseFI	GTSAC	1 cut(s)	85
TseI	GCWGC	1 cut(s)	1490
Tsp45I	GTSAC	1 cut(s)	85
TspDTI	ATGAA	8 cut(s)	273, 305, 314, 374, 560, 608, 1092, 1340
TspGWI	ACGGA	1 cut(s)	645
TspRI	CASTG	3 cut(s)	991, 1276, 1396
Vha464I	CTTAAG	1 cut(s)	1627
VpaK11BI	GGWCC	1 cut(s)	288
VspI	ATTAAT	3 cut(s)	786, 996, 1101
XapI	RAATTY	4 cut(s)	260, 595, 1195, 1441
XceI	RCATGY	1 cut(s)	1241
XhoI	CTCGAG	2 cut(s)	744, 1139
XspI	CTAG	2 cut(s)	1265, 1332
Zsp2I	ATGCAT	1 cut(s)	1325

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.