Rroxscaffold_3G00244980

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0003055
Physical Location & Seq
Forward (+)
37783775 .. 37786096
2322 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00244980.1

Sequence Viewer

Length: 2322 bp
ATGGATTTGAGAAGGCTTCCGATCTCCAAACCCGCTTCTCTCTTCTACCCAATCACACGCCCCTTAACGGGCGTGACGTTCACCTTCCAACGCCGCCACGACCCTCCCCGGCCGCCGAGTCCACCACAGCCTCCCAATCCGGGTTTGCTCAACTGGGTATCTTCTATTCTCTCAAACCCATCTTTGGATTCTTCTAAATGTGAATCTCTCATGCCCCTTTTGTCCCCTCATGAATTTGATCAGTTATTCTACTCCATTAGGTCCAATGTGAACCCCAAAACAGCTCTTCATTTCTTTCATTTTGCTTCTCAATCTTTTAAGTTTCGGTTTACTGTTCGATCTTTCTGTGTTCTGGTTCATTTGCTCATTGCTTCAAATCTTGGGCCTCCTGCGAGGTTGCTTTTGATTCGTTTGATTGATGGGAATGTCCCAGTTTTGTGTGCTGACCCCAATAGAAAGCATATTGAGATAGCCGTTGCAATGTCGGAGTTGAACAATGTGTCCCAACCGGCTCTGGGGGTTCAGGCATTGGATATGTTAATTCATGTTTACTGTACCCAGTTCAAGAATTTGGGTTTTGGTTATGCTGTTGATGTGTTTGAGCATTTTTCGGATAAGGGTGTGTTTCCGTCTTTGAAGACTTGTAATGTTTTGCTGAGTTCTTTAGTGAAGGCTAACGAACTTGAGAAGAGCTATCATGTATTTGAAGTTATGACTCGAGGTGTTTCTCCTGATGTTTTCTTGTTTACTACTGCAATTAATGCGTTTTGCAAGGGAGGGAAGGTGGATGATGCTATAGCTGTGTTCTCAAAAATGGAGAGCATTGGTGTTGCTCCAAATGTGGTTACTTACAATAGCGTTATCCACGGGCTATGTAAGAGTAGAAGATTAGAGGCGGCTTTCCAGTTTAAGGAGAAGATGGAAAAGAATAATGTGAAGCCGAGTCTTATAACATACAGTGTGCTCATTAATGGTTTGATTAAGCTGGAGAAGTTTTATGAGGCAAATTGTGTTTTCACGGAAATGCGTAGTTGGGGGTTTGTCCCGAATGAGGTTGTATATAACACTCTGATTAATGGGTATTGTACAATGGGAAATATTGGCGAGGCACTCAAGATAAGGGATGATATGGTATCAAATGGGGTTACCCCTAATTCTGTTACTCTGAATTCGCTACTGCATGGATTTTGTAAAAGTAATCAATTTGAGCATGCTGAGCAATTTTTGGACAAGATGCTAGCCAGTGGCTTACCAGTCAACCAAGTTGTTTCTTACTCAGTCATTGACTGGTTATGCATGAAATGTAGGTTTGATTCTGCACTAAAATTCACTACTGAAATGTTATTAAGAAACTTTAGGCCCAGTGATAGTTTGCTTACTACATTGTTTGTTGGGCTCTGCAAAGATGGGAAGAATTTAGAGGCAATCGAACTTTGGTTTAGGCTATCGGAGAAGGGGTTTGCAGCCAACACAGCGATCACAAATGCTCTAATCCATGGACTTTGTGAATCTGGTAGCATCGAAGAGGTTGTTAGGCTACTCAAGGCAATGCTTGAGAGGGGTTTGGCATTGGATAAGTTCTCATACAACACACTCATCTTAAGTTGTTGCAAGGAGGGAAGAGTGGCAGAAGGTTTTAAGCTTAGAGAAGAGATGTATAAGCAAGGAATTGATCCTGACATCAACACTTACAATATGCTACTGCATGGTCTATGTAGTATGGGCAAAGTGGACGATGCTGTTAAACTTTGGGTTGAATGCGAAAATCATGGTCTTGTGGCCAACATTTATACATATGAGGTGATGATAGAAGGGTACTGTAACTCTGGTAGAATCAAAGATGCTGAACTCCTGTTTAGTAAGTTGGTGACTAAGAAAGTGGAGCCAAATTCCGTTGTTTATAATACACTAATCAGTGCATATTGTATAGATGGGAATATGATTGGAGCTCTTGGCCTATTCCATGACATGAAAAACAAGGGCATTCAACCAACTTATGCCACATATTTTTCCCTTATAAATAGGCTATGCAATATTGGCCATGTCGAGGATGCAAAAGAGCTCATTGATGAAATGAGGAGGAATGGTTTGGTGCCAAATGTCACTTGTTATGCTGAAGCAATTTGTGGTTATTATAAGCTAGGCCAAATGGATAAAGCAAGGAGCATTTTTCGGGAGATGTCCTCATATAACATACAACCGAATGAAATTACATATACTGTCATGAGTGATGGGTATTGTAAGCTAGGCAACATGGAAGAAAAAAATATACTTCAATGTGAGATGGCAAAAAAGGGAATTGTCCCAGATGCAGTCACTTAG

Protein Analysis

773

Amino Acids

86.65

Weight (kDa)

7.45

Isoelectric Point (pI)

38.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 195 - 322 8.5e-10 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 210 - 258 4e-07 PPR repeat family
TPR_24 PF23276 234 - 340 3.8e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 236 - 289 2.5e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 240 - 273 2.5e-11 PPR repeat
PPR_2 PF13041 244 - 293 5.9e-16 PPR repeat family
PPR_3 PF13812 276 - 324 3e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 276 - 308 1.7e-11 PPR repeat
PPR_2 PF13041 279 - 328 1.9e-18 PPR repeat family
PPR PF01535 282 - 312 3.3e-06 PPR repeat
TPR_24 PF23276 338 - 423 5.5e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 338 - 394 2.5e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 346 - 378 6.6e-11 PPR repeat
PPR_2 PF13041 349 - 398 1.3e-17 PPR repeat family
PPR PF01535 352 - 382 1.8e-07 PPR repeat
PPR_1 PF12854 380 - 412 2.5e-09 PPR repeat
PPR_2 PF13041 384 - 422 5.2e-08 PPR repeat family
PPR PF01535 387 - 417 9.3e-06 PPR repeat
PPR_2 PF13041 490 - 537 1.8e-10 PPR repeat family
PPR_1 PF12854 520 - 552 2.2e-08 PPR repeat
PPR_2 PF13041 526 - 572 8.5e-16 PPR repeat family
PPR_1 PF12854 556 - 585 3.2e-10 PPR repeat
PPR_2 PF13041 560 - 608 8.2e-13 PPR repeat family
PPR_3 PF13812 584 - 641 2.5e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 591 - 621 3.1e-07 PPR repeat
PPR_2 PF13041 595 - 632 8.1e-07 PPR repeat family
PPR_long PF17177 618 - 742 8.7e-10 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 622 - 726 5.4e-08 Fungal tetratrico peptide repeats
PPR_1 PF12854 626 - 658 9.2e-08 PPR repeat
PPR_2 PF13041 629 - 678 4.2e-15 PPR repeat family
PPR PF01535 632 - 662 1.7e-08 PPR repeat
PPR_3 PF13812 653 - 705 3.5e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 660 - 693 4.4e-07 PPR repeat
PPR_2 PF13041 668 - 704 3e-07 PPR repeat family
PPR_2 PF13041 699 - 748 2.9e-13 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 950, 1902, 2018, 2136
AccB1I GGYRCC 1 cut(s) 2092
AciI CCGC 4 cut(s) 33, 94, 113, 896
AclWI GGATC 1 cut(s) 1667
AcoI YGGCCR 3 cut(s) 110, 1779, 2038
AcsI RAATTY 6 cut(s) 233, 568, 1168, 1325, 1414, 1888
AcuI CTGAAG 1 cut(s) 2136
AfaI GTAC 3 cut(s) 556, 1087, 1817
AfiI CCNNNNNNNGG 8 cut(s) 67, 68, 140, 184, 515, 910, 1051, 2047
AflII CTTAAG 1 cut(s) 1600
AgsI TTSAA 8 cut(s) 375, 493, 565, 637, 707, 1757, 1988, 2276
AloI GAACNNNNNNTCC 2 cut(s) 485, 517
AluBI AGCT 9 cut(s) 284, 693, 800, 985, 1642, 1949, 2062, 2140, 2245
AluI AGCT 9 cut(s) 284, 693, 800, 985, 1642, 1949, 2062, 2140, 2245
Alw21I GWGCWC 3 cut(s) 966, 1951, 2064
AlwI GGATC 1 cut(s) 1667
Ama87I CYCGRG 1 cut(s) 717
AoxI GGCC 7 cut(s) 110, 383, 1358, 1779, 1954, 2038, 2143
ApeKI GCWGC 1 cut(s) 1463
ApoI RAATTY 6 cut(s) 233, 568, 1168, 1325, 1414, 1888
AseI ATTAAT 3 cut(s) 759, 969, 1074
AspS9I GGNCC 3 cut(s) 261, 383, 1359
AsuC2I CCSGG 2 cut(s) 109, 141
AsuHPI GGTGA 3 cut(s) 73, 1813, 1879
AsuNHI GCTAGC 1 cut(s) 1237
AvaI CYCGRG 1 cut(s) 717
AvaII GGWCC 1 cut(s) 261
BalI TGGCCA 2 cut(s) 1781, 2040
BanI GGYRCC 1 cut(s) 2092
BanII GRGCYC 3 cut(s) 1398, 1951, 2064
BbsI GAAGAC 1 cut(s) 644
Bbv12I GWGCWC 3 cut(s) 966, 1951, 2064
BbvI GCAGC 1 cut(s) 1475
BccI CCATC 7 cut(s) 187, 413, 913, 1400, 1925, 2225, 2278
BceAI ACGGC 1 cut(s) 458
BclI TGATCA 1 cut(s) 238
BcnI CCSGG 2 cut(s) 109, 141
BfaI CTAG 3 cut(s) 1238, 2141, 2246
BfmI CTRYAG 1 cut(s) 795
BfrI CTTAAG 1 cut(s) 1600
BisI GCNGC 4 cut(s) 94, 113, 897, 1464
BlpI GCTNAGC 1 cut(s) 1215
BlsI GCNGC 4 cut(s) 95, 114, 898, 1465
Bme1390I CCNGG 2 cut(s) 109, 141
Bme18I GGWCC 1 cut(s) 261
BmeT110I CYCGRG 1 cut(s) 717
BmgT120I GGNCC 3 cut(s) 261, 383, 1359
BmiI GGNNCC 2 cut(s) 1884, 2094
BmrFI CCNGG 2 cut(s) 109, 141
BmrI ACTGGG 4 cut(s) 163, 425, 553, 1356
BmsI GCATC 7 cut(s) 781, 1224, 1527, 1726, 1831, 2041, 2299
BmtI GCTAGC 1 cut(s) 1241
BmuI ACTGGG 4 cut(s) 163, 425, 553, 1356
BpiI GAAGAC 1 cut(s) 644
BplI GAGNNNNNCTC 2 cut(s) 2168, 2200
BpmI CTGGAG 1 cut(s) 1007
Bpu1102I GCTNAGC 1 cut(s) 1215
BpuEI CTTGAG 4 cut(s) 704, 1097, 1526, 1574
BpuMI CCSGG 2 cut(s) 109, 141
BsaJI CCNNGG 3 cut(s) 107, 865, 1495
BsaXI ACNNNNNCTCC 6 cut(s) 768, 798, 1442, 1472, 2071, 2101
Bsc4I CCNNNNNNNGG 8 cut(s) 67, 68, 140, 184, 515, 910, 1051, 2047
Bse118I RCCGGY 1 cut(s) 508
Bse1I ACTGG 8 cut(s) 158, 431, 559, 904, 1242, 1253, 1292, 1362
Bse3DI GCAATG 3 cut(s) 366, 486, 1554
BseDI CCNNGG 3 cut(s) 107, 865, 1495
BseGI GGATG 3 cut(s) 793, 1129, 2056
BseLI CCNNNNNNNGG 8 cut(s) 67, 68, 140, 184, 515, 910, 1051, 2047
BseMI GCAATG 3 cut(s) 366, 486, 1554
BseMII CTCAG 3 cut(s) 647, 1206, 1290
BseNI ACTGG 8 cut(s) 158, 431, 559, 904, 1242, 1253, 1292, 1362
BseRI GAGGAG 1 cut(s) 2092
BseX3I CGGCCG 1 cut(s) 110
BseXI GCAGC 1 cut(s) 1475
BsgI GTGCAG 1 cut(s) 1302
Bsh1285I CGRYCG 1 cut(s) 113
BshFI GGCC 7 cut(s) 112, 385, 1360, 1781, 1956, 2040, 2145
BshNI GGYRCC 1 cut(s) 2092
BsiEI CGRYCG 1 cut(s) 113
BsiHKAI GWGCWC 3 cut(s) 966, 1951, 2064
BsiHKCI CYCGRG 1 cut(s) 717
BsiSI CCGG 3 cut(s) 109, 140, 509
BslFI GGGAC 5 cut(s) 208, 413, 487, 1028, 2288
BslI CCNNNNNNNGG 8 cut(s) 67, 68, 140, 184, 515, 910, 1051, 2047
BsmFI GGGAC 5 cut(s) 208, 413, 487, 1028, 2288
BsmI GAATGC 2 cut(s) 1763, 1983
BsnI GGCC 7 cut(s) 112, 385, 1360, 1781, 1956, 2040, 2145
BsoBI CYCGRG 1 cut(s) 717
Bsp1286I GDGCHC 4 cut(s) 966, 1398, 1951, 2064
Bsp1407I TGTACA 1 cut(s) 1085
Bsp143I GATC 5 cut(s) 21, 238, 338, 1476, 1672
Bsp1720I GCTNAGC 1 cut(s) 1215
Bsp19I CCATGG 1 cut(s) 1495
BspACI CCGC 4 cut(s) 33, 94, 113, 896
BspANI GGCC 7 cut(s) 112, 385, 1360, 1781, 1956, 2040, 2145
BspCNI CTCAG 3 cut(s) 648, 1207, 1289
BspHI TCATGA 2 cut(s) 229, 2223
BspLI GGNNCC 2 cut(s) 1884, 2094
BspOI GCTAGC 1 cut(s) 1241
BspPI GGATC 1 cut(s) 1667
BspQI GCTCTTC 2 cut(s) 291, 683
BspT107I GGYRCC 1 cut(s) 2092
BspTI CTTAAG 1 cut(s) 1600
BsrDI GCAATG 3 cut(s) 366, 486, 1554
BsrFI RCCGGY 1 cut(s) 508
BsrGI TGTACA 1 cut(s) 1085
BsrI ACTGG 8 cut(s) 158, 431, 559, 904, 1242, 1253, 1292, 1362
BssAI RCCGGY 1 cut(s) 508
BssECI CCNNGG 3 cut(s) 107, 865, 1495
BssMI GATC 5 cut(s) 21, 238, 338, 1476, 1672
BssT1I CCWWGG 1 cut(s) 1495
Bst4CI ACNGT 5 cut(s) 334, 554, 959, 1820, 2221
Bst6I CTCTTC 6 cut(s) 47, 291, 683, 1518, 1615, 1644
BstAFI CTTAAG 1 cut(s) 1600
BstAPI GCANNNNNTGC 1 cut(s) 761
BstAUI TGTACA 1 cut(s) 1085
BstC8I GCNNGC 2 cut(s) 1212, 1239
BstDEI CTNAG 6 cut(s) 656, 1215, 1276, 1643, 1872, 2319
BstDSI CCRYGG 2 cut(s) 865, 1495
BstEII GGTNACC 1 cut(s) 1144
BstF5I GGATG 3 cut(s) 793, 1129, 2056
BstKTI GATC 5 cut(s) 24, 241, 341, 1479, 1675
BstMBI GATC 5 cut(s) 21, 238, 338, 1476, 1672
BstMCI CGRYCG 1 cut(s) 113
BstMWI GCNNNNNNNGC 4 cut(s) 761, 1216, 1472, 2037
BstNSI RCATGY 1 cut(s) 1214
BstPI GGTNACC 1 cut(s) 1144
BstSCI CCNGG 2 cut(s) 107, 139
BstSFI CTRYAG 1 cut(s) 795
BstV1I GCAGC 1 cut(s) 1475
BstV2I GAAGAC 1 cut(s) 644
BstZI CGGCCG 1 cut(s) 110
BsuRI GGCC 7 cut(s) 112, 385, 1360, 1781, 1956, 2040, 2145
BtgI CCRYGG 2 cut(s) 865, 1495
BtsCI GGATG 3 cut(s) 793, 1129, 2056
BtsIMutI CAGTG 4 cut(s) 964, 1249, 1369, 1921
Cac8I GCNNGC 2 cut(s) 1212, 1239
CciI TCATGA 2 cut(s) 229, 2223
Cfr10I RCCGGY 1 cut(s) 508
Cfr13I GGNCC 3 cut(s) 261, 383, 1359
Csp6I GTAC 3 cut(s) 555, 1086, 1816
CviQI GTAC 3 cut(s) 555, 1086, 1816
DdeI CTNAG 6 cut(s) 656, 1215, 1276, 1643, 1872, 2319
DpnI GATC 5 cut(s) 23, 240, 340, 1478, 1674
DpnII GATC 5 cut(s) 21, 238, 338, 1476, 1672
EaeI YGGCCR 3 cut(s) 110, 1779, 2038
EagI CGGCCG 1 cut(s) 110
Eam1104I CTCTTC 6 cut(s) 47, 291, 683, 1518, 1615, 1644
EarI CTCTTC 6 cut(s) 47, 291, 683, 1518, 1615, 1644
Ecl136II GAGCTC 2 cut(s) 1949, 2062
EclXI CGGCCG 1 cut(s) 110
Eco130I CCWWGG 1 cut(s) 1495
Eco24I GRGCYC 3 cut(s) 1398, 1951, 2064
Eco47I GGWCC 1 cut(s) 261
Eco52I CGGCCG 1 cut(s) 110
Eco53kI GAGCTC 2 cut(s) 1949, 2062
Eco57I CTGAAG 1 cut(s) 2136
Eco88I CYCGRG 1 cut(s) 717
Eco91I GGTNACC 1 cut(s) 1144
EcoICRI GAGCTC 2 cut(s) 1949, 2062
EcoO65I GGTNACC 1 cut(s) 1144
EcoRI GAATTC 1 cut(s) 1168
EcoT14I CCWWGG 1 cut(s) 1495
EcoT22I ATGCAT 1 cut(s) 1298
EcoT38I GRGCYC 3 cut(s) 1398, 1951, 2064
ErhI CCWWGG 1 cut(s) 1495
FaqI GGGAC 5 cut(s) 208, 413, 487, 1028, 2288
FauI CCCGC 1 cut(s) 40
FauNDI CATATG 1 cut(s) 1795
FbaI TGATCA 1 cut(s) 238
Fnu4HI GCNGC 4 cut(s) 94, 113, 897, 1464
FokI GGATG 3 cut(s) 800, 1136, 2063
FriOI GRGCYC 3 cut(s) 1398, 1951, 2064
Fsp4HI GCNGC 4 cut(s) 94, 113, 897, 1464
FspBI CTAG 3 cut(s) 1238, 2141, 2246
GluI GCNGC 4 cut(s) 94, 113, 897, 1464
GsuI CTGGAG 1 cut(s) 1007
HaeIII GGCC 7 cut(s) 112, 385, 1360, 1781, 1956, 2040, 2145
HapII CCGG 3 cut(s) 109, 140, 509
HincII GTYRAC 1 cut(s) 1258
HindII GTYRAC 1 cut(s) 1258
HindIII AAGCTT 1 cut(s) 1640
HinfI GANTC 9 cut(s) 118, 188, 203, 406, 715, 943, 1313, 1508, 1833
HpaII CCGG 3 cut(s) 109, 140, 509
HphI GGTGA 3 cut(s) 73, 1813, 1879
Hpy166II GTNNAC 8 cut(s) 81, 122, 271, 330, 550, 747, 1258, 1732
Hpy188I TCNGA 6 cut(s) 21, 487, 613, 1071, 1167, 1450
Hpy188III TCNNGA 8 cut(s) 230, 565, 731, 1045, 1114, 1676, 2174, 2224
Hpy8I GTNNAC 8 cut(s) 81, 122, 271, 330, 550, 747, 1258, 1732
HpyAV CCTTC 7 cut(s) 6, 94, 664, 775, 1447, 1625, 1805
HpyCH4III ACNGT 5 cut(s) 334, 554, 959, 1820, 2221
HpyCH4IV ACGT 1 cut(s) 77
HpyF10VI GCNNNNNNNGC 4 cut(s) 761, 1216, 1472, 2037
HpyF3I CTNAG 6 cut(s) 656, 1215, 1276, 1643, 1872, 2319
HpySE526I ACGT 1 cut(s) 77
Ksp22I TGATCA 1 cut(s) 238
Kzo9I GATC 5 cut(s) 21, 238, 338, 1476, 1672
LguI GCTCTTC 2 cut(s) 291, 683
LmnI GCTCC 4 cut(s) 838, 1882, 1946, 2163
Lsp1109I GCAGC 1 cut(s) 1475
LweI GCATC 7 cut(s) 781, 1224, 1527, 1726, 1831, 2041, 2299
MaeI CTAG 3 cut(s) 1238, 2141, 2246
MaeII ACGT 1 cut(s) 77
MaeIII GTNAC 8 cut(s) 73, 844, 1144, 1159, 1820, 1867, 2101, 2314
MalI GATC 5 cut(s) 23, 240, 340, 1478, 1674
MboI GATC 5 cut(s) 21, 238, 338, 1476, 1672
MhlI GDGCHC 4 cut(s) 966, 1398, 1951, 2064
MlsI TGGCCA 2 cut(s) 1781, 2040
MluNI TGGCCA 2 cut(s) 1781, 2040
MlyI GAGTC 3 cut(s) 127, 709, 952
MmeI TCCRAC 2 cut(s) 112, 465
Mox20I TGGCCA 2 cut(s) 1781, 2040
Mph1103I ATGCAT 1 cut(s) 1298
MscI TGGCCA 2 cut(s) 1781, 2040
MslI CAYNNNNRTG 1 cut(s) 1022
Msp20I TGGCCA 2 cut(s) 1781, 2040
MspCI CTTAAG 1 cut(s) 1600
MspI CCGG 3 cut(s) 109, 140, 509
MspR9I CCNGG 2 cut(s) 109, 141
Mva1269I GAATGC 2 cut(s) 1763, 1983
MwoI GCNNNNNNNGC 4 cut(s) 761, 1216, 1472, 2037
NciI CCSGG 2 cut(s) 109, 141
NcoI CCATGG 1 cut(s) 1495
NdeI CATATG 1 cut(s) 1795
NdeII GATC 5 cut(s) 21, 238, 338, 1476, 1672
NheI GCTAGC 1 cut(s) 1237
NlaIV GGNNCC 2 cut(s) 1884, 2094
NmeAIII GCCGAG 2 cut(s) 141, 966
NmuCI GTSAC 4 cut(s) 73, 1867, 2101, 2314
NsiI ATGCAT 1 cut(s) 1298
NspI RCATGY 1 cut(s) 1214
PaeI GCATGC 1 cut(s) 1214
PaeR7I CTCGAG 1 cut(s) 717
PagI TCATGA 2 cut(s) 229, 2223
PciSI GCTCTTC 2 cut(s) 291, 683
PcsI WCGNNNNNNNCGW 1 cut(s) 74
PctI GAATGC 2 cut(s) 1763, 1983
PfeI GAWTC 6 cut(s) 188, 203, 406, 1313, 1508, 1833
PkrI GCNGC 4 cut(s) 95, 114, 898, 1465
PleI GAGTC 3 cut(s) 126, 709, 951
PpsI GAGTC 3 cut(s) 126, 709, 951
PshBI ATTAAT 3 cut(s) 759, 969, 1074
PsiI TTATAA 4 cut(s) 950, 1902, 2018, 2136
Psp124BI GAGCTC 2 cut(s) 1951, 2064
PspEI GGTNACC 1 cut(s) 1144
PspN4I GGNNCC 2 cut(s) 1884, 2094
PspPI GGNCC 3 cut(s) 261, 383, 1359
PspXI VCTCGAGB 1 cut(s) 717
RsaI GTAC 3 cut(s) 556, 1087, 1817
RsaNI GTAC 3 cut(s) 555, 1086, 1816
RseI CAYNNNNRTG 1 cut(s) 1022
SacI GAGCTC 2 cut(s) 1951, 2064
SapI GCTCTTC 2 cut(s) 291, 683
SatI GCNGC 4 cut(s) 94, 113, 897, 1464
Sau3AI GATC 5 cut(s) 21, 238, 338, 1476, 1672
Sau96I GGNCC 3 cut(s) 261, 383, 1359
SchI GAGTC 3 cut(s) 127, 709, 952
ScrFI CCNGG 2 cut(s) 109, 141
SduI GDGCHC 4 cut(s) 966, 1398, 1951, 2064
SfaNI GCATC 7 cut(s) 781, 1224, 1527, 1726, 1831, 2041, 2299
SfcI CTRYAG 1 cut(s) 795
Sfr274I CTCGAG 1 cut(s) 717
SinI GGWCC 1 cut(s) 261
SlaI CTCGAG 1 cut(s) 717
SmiMI CAYNNNNRTG 1 cut(s) 1022
SmlI CTYRAG 6 cut(s) 683, 717, 1112, 1541, 1553, 1600
SmoI CTYRAG 6 cut(s) 683, 717, 1112, 1541, 1553, 1600
SphI GCATGC 1 cut(s) 1214
SsiI CCGC 4 cut(s) 33, 94, 113, 896
SspI AATATT 2 cut(s) 1099, 2035
SspMI CTAG 3 cut(s) 1238, 2141, 2246
SstI GAGCTC 2 cut(s) 1951, 2064
StyD4I CCNGG 2 cut(s) 107, 139
StyI CCWWGG 1 cut(s) 1495
TaaI ACNGT 5 cut(s) 334, 554, 959, 1820, 2221
TaiI ACGT 1 cut(s) 80
TaqI TCGA 5 cut(s) 337, 718, 1428, 1521, 2046
TatI WGTACW 1 cut(s) 1085
TauI GCSGC 3 cut(s) 96, 115, 899
TfiI GAWTC 6 cut(s) 188, 203, 406, 1313, 1508, 1833
TscAI CASTG 4 cut(s) 964, 1249, 1369, 1921
TseFI GTSAC 4 cut(s) 73, 1867, 2101, 2314
TseI GCWGC 1 cut(s) 1463
Tsp45I GTSAC 4 cut(s) 73, 1867, 2101, 2314
TspDTI ATGAA 9 cut(s) 246, 278, 287, 347, 533, 1313, 1985, 2085, 2220
TspGWI ACGGA 3 cut(s) 618, 1034, 1882
TspRI CASTG 4 cut(s) 964, 1249, 1369, 1921
Vha464I CTTAAG 1 cut(s) 1600
VpaK11BI GGWCC 1 cut(s) 261
VspI ATTAAT 3 cut(s) 759, 969, 1074
XapI RAATTY 6 cut(s) 233, 568, 1168, 1325, 1414, 1888
XceI RCATGY 1 cut(s) 1214
XhoI CTCGAG 1 cut(s) 717
XspI CTAG 3 cut(s) 1238, 2141, 2246
Zsp2I ATGCAT 1 cut(s) 1298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.