Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7A

Orthogroup ID

OG0003055

Physical Location & Seq

Genomic Coordinates Reverse (-)

30453708 .. 30455291

Gene Structure 1584 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7AG284500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1584 bp

ATGCAATTCTCAATGGATTTGAGAAGGCTTCCGATCTCCAAACCCGCTTCTCTCTTCTACCCAATCACGCGCCCCTTAACGGGCGTGACATTCACCTTCCAACGCCGCCACTACCCTCCCCAGCCGCCGCCGTTACAGCCTTTGAGTCCACCACGGCCTCCCAATCCGGTTTTGCTCAACTGGGTATCTTCTATTCTCTCAAACCCATCTTTGGATTCTTCTAAATGTGAATCTCTCATGCCCTTTTTGTCCCCTCATGAATTTGATCAGTTATTCTACTCCATTAGGTCCAATGTGAACCCCAAAACAGCTCTTCATTTCTTTCATTTTGCTTCTCAATCTTTTAAGTTTCGGTTTACTGTTCGATCTTTCTGTGTTTTGGTTCATTTGCTCATTGCTTCAAATCTTAGACCTCCTGCGAGGTTGCTTTTGATTCGTTTGATTGATGGGAATGTCCCAGTTTTGTGTGCTGACCCCAACAGCAAGCAAATTGAGATAGCCGTCGCAATGTCGGAGTTGAACAATGCGTCCCAACCGGCTCTGGGGATTCAAGCATTGGATATGTTAATTCATGTTTACTGTACCCAGTTCAAGAATTTGGGTTTTGGTTATGCTGTTGATGTGTTTGAGCATTTTTCGGATAAGGGTGTGTTTCCGTCTTTGAAGACTTGTAATGTTTTGCTGAGTTCTTTAGTGAAGGCTAACGAACTTGAGAAGAGCTGTCATGTATTTGAAGTTATGACTCGAGGTGTTTCTCCTAATGTTTTCTTGTTTACTACTGCAATTAATGTGTTTTGCAAGGGAGGGAAGGTGGATGATGCAATAGCTGTGCTCTCAAAAATGGAGAGCATTGGTGTTGCTCCAAATGTGGTTACTTACAATAGCGTTATCCACGGGCTATGTATGAGTAGAAGATTAGAGGAGGCTTTCCGGTTCAAGGAGAAGATGGTAAAGAATAATGTGAAGCCAAGTCTTATAACATACAATGTGCTCATTAATGGTTTAATTAAGCTGGAGAAGTTTTATGAGGCAAATTGTGTTTTCAAGGAAATGCGTAGTTGGGGATTTATCCCGAATGAGGTTGTATATAACACTCTGATTAATGGGTATTGTACAATGGGAAATATTGGAGAGGCACTCAAGATAAGGGATGATATGGTATCGAATGGGGTTACCCCTAATTCTGTTACTCTGAATTCGCTACTGCATGGATATTGTAAAAGTAATCAATTTGAGCATGCTGAGCAATTTTTGGACCAGATGCTAGCCAGTGGTTTACCAGTCAACCAAGTTGTTTCTTACTCAGTCATTGACTGGTTATGCATGAAATTTAGGTTTGATTCTACACTAAGATTCACTACCGAAATGTTTTTAAGAAACTTTAGGCCCAGTGATAGTTTGCTTACTACATTGTTTGTTGGGCTATGCAAAGATGGGAAGAATTTAGAGGCAATCAAACTTTGGTTTAGGCTATCGGAGAAGGGGTTTGCAGCCAACACAGCGACCACAAATGCTCTAATCCATGGACTTTGTGAATCTGGTAGTATCGAAGAGGTTGTTAGGCTACTCAAGGCAATGCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

527

Amino Acids

59.26

Weight (kDa)

8.89

Isoelectric Point (pI)

43.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_long	PF17177	204 - 332	1.7e-10	Pentacotripeptide-repeat region of PRORP
TPR_24	PF23276	243 - 350	6.2e-06	Fungal tetratrico peptide repeats
PPR_3	PF13812	245 - 298	5.8e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	249 - 282	2e-10	PPR repeat
PPR_2	PF13041	253 - 301	2.3e-14	PPR repeat family
PPR_3	PF13812	277 - 333	1.2e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	285 - 317	2.2e-11	PPR repeat
PPR_2	PF13041	288 - 337	5e-18	PPR repeat family
PPR	PF01535	291 - 321	1.9e-06	PPR repeat
PPR_3	PF13812	313 - 370	5.6e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	323 - 361	1.7e-08	PPR repeat family
PPR	PF01535	326 - 356	6.5e-06	PPR repeat
PPR_3	PF13812	347 - 405	1e-07	Pentatricopeptide repeat domain
TPR_24	PF23276	347 - 432	8.5e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	355 - 387	1.6e-10	PPR repeat
PPR_2	PF13041	358 - 407	1.2e-17	PPR repeat family
PPR	PF01535	361 - 391	1.1e-07	PPR repeat
PPR_1	PF12854	389 - 421	1.4e-09	PPR repeat
PPR_2	PF13041	393 - 441	2e-09	PPR repeat family
PPR	PF01535	396 - 426	2.1e-06	PPR repeat
PPR_2	PF13041	469 - 511	1.3e-06	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003055)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G19440 AT4G19440 AT4G19440 AT4G19440
fragaria_vesca	FvH4_4g10410 FvH4_4g10410 FvH4_4g10440 FvH4_5g24430 FvH4_5g24430 FvH4_5g24430 FvH4_5g24430
malus_domestica	MD06G1022200.v1.1
prunus_persica	Prupe.3G018200_v2.0.a1
pyrus_communis	pycom06g01610
rosa_chinensis	RchiOBHm_Chr4g0405931 RchiOBHm_Chr7g0214821 RchiOBHm_Chr7g0219861
rosa_laevigata	RLG00000002716 RLG00000008810
rosa_multiflora	Rmu_sc0002308.1_g000021 Rmu_sc0019702.1_g000004 Rmu_ssc0000087.1_g000071
rosa_roxburghii	Rroxscaffold_3G00244980 Rroxscaffold_5G00350670
rosa_rugosa	Rorug04G0064100 Rorug07G0147600 Rorug07G0147700 Rorug07G0147800
rosa_samantha	Rh4AG134900 Rh4BG130500 Rh4CG142300 Rh4DG129200 Rh7AG284500 Rh7BG275300 Rh7CG303700 Rh7DG287900
rosa_wichuraiana	Rw4G010980 Rw7G024260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	977
AccII	CGCG	1 cut(s)	70
AciI	CCGC	4 cut(s)	45, 106, 125, 128
AcsI	RAATTY	5 cut(s)	260, 595, 1195, 1328, 1441
AfaI	GTAC	2 cut(s)	583, 1114
AfiI	CCNNNNNNNGG	6 cut(s)	79, 80, 211, 542, 937, 1078
AgsI	TTSAA	8 cut(s)	402, 520, 551, 592, 664, 734, 937, 1045
AloI	GAACNNNNNNTCC	2 cut(s)	512, 544
AluBI	AGCT	4 cut(s)	311, 720, 827, 1012
AluI	AGCT	4 cut(s)	311, 720, 827, 1012
Alw21I	GWGCWC	2 cut(s)	834, 993
Ama87I	CYCGRG	1 cut(s)	744
AoxI	GGCC	2 cut(s)	155, 1385
ApeKI	GCWGC	1 cut(s)	1490
ApoI	RAATTY	5 cut(s)	260, 595, 1195, 1328, 1441
AseI	ATTAAT	3 cut(s)	786, 996, 1101
AspLEI	GCGC	1 cut(s)	72
AspS9I	GGNCC	3 cut(s)	288, 1255, 1386
AsuHPI	GGTGA	1 cut(s)	85
AsuNHI	GCTAGC	1 cut(s)	1264
AvaI	CYCGRG	1 cut(s)	744
AvaII	GGWCC	2 cut(s)	288, 1255
BbsI	GAAGAC	1 cut(s)	671
Bbv12I	GWGCWC	2 cut(s)	834, 993
BbvI	GCAGC	1 cut(s)	1502
BccI	CCATC	4 cut(s)	214, 440, 940, 1427
BceAI	ACGGC	3 cut(s)	115, 170, 485
BclI	TGATCA	1 cut(s)	265
BfaI	CTAG	1 cut(s)	1265
BisI	GCNGC	4 cut(s)	106, 125, 128, 1491
BlpI	GCTNAGC	1 cut(s)	1242
BlsI	GCNGC	4 cut(s)	107, 126, 129, 1492
Bme18I	GGWCC	2 cut(s)	288, 1255
BmeT110I	CYCGRG	1 cut(s)	744
BmgT120I	GGNCC	3 cut(s)	288, 1255, 1386
BmrI	ACTGGG	4 cut(s)	190, 452, 580, 1383
BmsI	GCATC	2 cut(s)	808, 1251
BmtI	GCTAGC	1 cut(s)	1268
BmuI	ACTGGG	4 cut(s)	190, 452, 580, 1383
BpiI	GAAGAC	1 cut(s)	671
BplI	GAGNNNNNCTC	2 cut(s)	1122, 1154
BpmI	CTGGAG	1 cut(s)	1034
Bpu1102I	GCTNAGC	1 cut(s)	1242
BpuEI	CTTGAG	3 cut(s)	731, 1124, 1553
BsaJI	CCNNGG	3 cut(s)	152, 892, 1522
BsaWI	WCCGGW	2 cut(s)	166, 930
BsaXI	ACNNNNNCTCC	4 cut(s)	795, 825, 1469, 1499
Bsc4I	CCNNNNNNNGG	6 cut(s)	79, 80, 211, 542, 937, 1078
Bse118I	RCCGGY	1 cut(s)	535
Bse1I	ACTGG	7 cut(s)	185, 458, 586, 1269, 1280, 1319, 1389
Bse3DI	GCAATG	3 cut(s)	393, 513, 1581
BseDI	CCNNGG	3 cut(s)	152, 892, 1522
BseGI	GGATG	2 cut(s)	820, 1156
BseLI	CCNNNNNNNGG	6 cut(s)	79, 80, 211, 542, 937, 1078
BseMI	GCAATG	3 cut(s)	393, 513, 1581
BseMII	CTCAG	3 cut(s)	674, 1233, 1317
BseNI	ACTGG	7 cut(s)	185, 458, 586, 1269, 1280, 1319, 1389
BseRI	GAGGAG	1 cut(s)	935
BseXI	GCAGC	1 cut(s)	1502
BseYI	CCCAGC	1 cut(s)	120
Bsh1236I	CGCG	1 cut(s)	70
BshFI	GGCC	2 cut(s)	157, 1387
BsiHKAI	GWGCWC	2 cut(s)	834, 993
BsiHKCI	CYCGRG	1 cut(s)	744
BsiSI	CCGG	3 cut(s)	167, 536, 931
BslFI	GGGAC	3 cut(s)	235, 440, 514
BslI	CCNNNNNNNGG	6 cut(s)	79, 80, 211, 542, 937, 1078
BsmFI	GGGAC	3 cut(s)	235, 440, 514
BsnI	GGCC	2 cut(s)	157, 1387
BsoBI	CYCGRG	1 cut(s)	744
Bsp1286I	GDGCHC	2 cut(s)	834, 993
Bsp1407I	TGTACA	1 cut(s)	1112
Bsp143I	GATC	3 cut(s)	33, 265, 365
Bsp1720I	GCTNAGC	1 cut(s)	1242
Bsp19I	CCATGG	1 cut(s)	1522
BspACI	CCGC	4 cut(s)	45, 106, 125, 128
BspANI	GGCC	2 cut(s)	157, 1387
BspCNI	CTCAG	3 cut(s)	675, 1234, 1316
BspFNI	CGCG	1 cut(s)	70
BspHI	TCATGA	1 cut(s)	256
BspOI	GCTAGC	1 cut(s)	1268
BspQI	GCTCTTC	2 cut(s)	318, 710
BsrDI	GCAATG	3 cut(s)	393, 513, 1581
BsrFI	RCCGGY	1 cut(s)	535
BsrGI	TGTACA	1 cut(s)	1112
BsrI	ACTGG	7 cut(s)	185, 458, 586, 1269, 1280, 1319, 1389
BssAI	RCCGGY	1 cut(s)	535
BssECI	CCNNGG	3 cut(s)	152, 892, 1522
BssMI	GATC	3 cut(s)	33, 265, 365
BssT1I	CCWWGG	1 cut(s)	1522
Bst4CI	ACNGT	2 cut(s)	361, 581
Bst6I	CTCTTC	4 cut(s)	59, 318, 710, 1545
BstAUI	TGTACA	1 cut(s)	1112
BstC8I	GCNNGC	3 cut(s)	485, 1239, 1266
BstDEI	CTNAG	5 cut(s)	407, 683, 1242, 1303, 1349
BstDSI	CCRYGG	3 cut(s)	152, 892, 1522
BstEII	GGTNACC	1 cut(s)	1171
BstF5I	GGATG	2 cut(s)	820, 1156
BstFNI	CGCG	1 cut(s)	70
BstHHI	GCGC	1 cut(s)	72
BstKTI	GATC	3 cut(s)	36, 268, 368
BstMBI	GATC	3 cut(s)	33, 265, 365
BstMWI	GCNNNNNNNGC	3 cut(s)	136, 1243, 1499
BstNSI	RCATGY	1 cut(s)	1241
BstPI	GGTNACC	1 cut(s)	1171
BstUI	CGCG	1 cut(s)	70
BstV1I	GCAGC	1 cut(s)	1502
BstV2I	GAAGAC	1 cut(s)	671
BsuRI	GGCC	2 cut(s)	157, 1387
BtgI	CCRYGG	3 cut(s)	152, 892, 1522
BtsCI	GGATG	2 cut(s)	820, 1156
BtsIMutI	CAGTG	2 cut(s)	1276, 1396
Cac8I	GCNNGC	3 cut(s)	485, 1239, 1266
CciI	TCATGA	1 cut(s)	256
CfoI	GCGC	1 cut(s)	72
Cfr10I	RCCGGY	1 cut(s)	535
Cfr13I	GGNCC	3 cut(s)	288, 1255, 1386
CseI	GACGC	1 cut(s)	516
Csp6I	GTAC	2 cut(s)	582, 1113
CviAII	CATG	8 cut(s)	238, 257, 572, 725, 1208, 1238, 1324, 1523
CviQI	GTAC	2 cut(s)	582, 1113
DdeI	CTNAG	5 cut(s)	407, 683, 1242, 1303, 1349
DpnI	GATC	3 cut(s)	35, 267, 367
DpnII	GATC	3 cut(s)	33, 265, 365
Eam1104I	CTCTTC	4 cut(s)	59, 318, 710, 1545
EarI	CTCTTC	4 cut(s)	59, 318, 710, 1545
Eco130I	CCWWGG	1 cut(s)	1522
Eco47I	GGWCC	2 cut(s)	288, 1255
Eco88I	CYCGRG	1 cut(s)	744
Eco91I	GGTNACC	1 cut(s)	1171
EcoO65I	GGTNACC	1 cut(s)	1171
EcoRI	GAATTC	1 cut(s)	1195
EcoT14I	CCWWGG	1 cut(s)	1522
EcoT22I	ATGCAT	1 cut(s)	1325
ErhI	CCWWGG	1 cut(s)	1522
FaeI	CATG	8 cut(s)	241, 260, 575, 728, 1211, 1241, 1327, 1526
FaqI	GGGAC	3 cut(s)	235, 440, 514
FatI	CATG	8 cut(s)	237, 256, 571, 724, 1207, 1237, 1323, 1522
FauI	CCCGC	1 cut(s)	52
FbaI	TGATCA	1 cut(s)	265
Fnu4HI	GCNGC	4 cut(s)	106, 125, 128, 1491
FokI	GGATG	2 cut(s)	827, 1163
Fsp4HI	GCNGC	4 cut(s)	106, 125, 128, 1491
FspBI	CTAG	1 cut(s)	1265
GlaI	GCGC	1 cut(s)	71
GluI	GCNGC	4 cut(s)	106, 125, 128, 1491
GsaI	CCCAGC	1 cut(s)	124
GsuI	CTGGAG	1 cut(s)	1034
HaeIII	GGCC	2 cut(s)	157, 1387
HapII	CCGG	3 cut(s)	167, 536, 931
HgaI	GACGC	1 cut(s)	516
HhaI	GCGC	1 cut(s)	72
Hin1II	CATG	8 cut(s)	241, 260, 575, 728, 1211, 1241, 1327, 1526
Hin6I	GCGC	1 cut(s)	70
HinP1I	GCGC	1 cut(s)	70
HincII	GTYRAC	1 cut(s)	1285
HindII	GTYRAC	1 cut(s)	1285
HinfI	GANTC	9 cut(s)	145, 215, 230, 433, 547, 742, 1340, 1353, 1535
HpaII	CCGG	3 cut(s)	167, 536, 931
HphI	GGTGA	1 cut(s)	85
Hpy166II	GTNNAC	7 cut(s)	149, 298, 357, 577, 774, 1277, 1285
Hpy188I	TCNGA	6 cut(s)	33, 514, 640, 1098, 1194, 1477
Hpy188III	TCNNGA	4 cut(s)	257, 592, 1072, 1141
Hpy8I	GTNNAC	7 cut(s)	149, 298, 357, 577, 774, 1277, 1285
Hpy99I	CGWCG	1 cut(s)	506
HpyAV	CCTTC	5 cut(s)	18, 106, 691, 802, 1474
HpyCH4III	ACNGT	2 cut(s)	361, 581
HpyCH4V	TGCA	8 cut(s)	4, 782, 798, 821, 1207, 1323, 1428, 1490
HpyF10VI	GCNNNNNNNGC	3 cut(s)	136, 1243, 1499
HpyF3I	CTNAG	5 cut(s)	407, 683, 1242, 1303, 1349
Hsp92II	CATG	8 cut(s)	241, 260, 575, 728, 1211, 1241, 1327, 1526
HspAI	GCGC	1 cut(s)	70
Ksp22I	TGATCA	1 cut(s)	265
Kzo9I	GATC	3 cut(s)	33, 265, 365
LguI	GCTCTTC	2 cut(s)	318, 710
LmnI	GCTCC	1 cut(s)	865
Lsp1109I	GCAGC	1 cut(s)	1502
LweI	GCATC	2 cut(s)	808, 1251
MaeI	CTAG	1 cut(s)	1265
MaeIII	GTNAC	5 cut(s)	85, 132, 871, 1171, 1186
MalI	GATC	3 cut(s)	35, 267, 367
MboI	GATC	3 cut(s)	33, 265, 365
MhlI	GDGCHC	2 cut(s)	834, 993
MlyI	GAGTC	2 cut(s)	154, 736
MmeI	TCCRAC	2 cut(s)	124, 492
Mph1103I	ATGCAT	1 cut(s)	1325
MseI	TTAA	9 cut(s)	77, 345, 566, 786, 996, 1004, 1008, 1101, 1373
MspI	CCGG	3 cut(s)	167, 536, 931
MvnI	CGCG	1 cut(s)	70
MwoI	GCNNNNNNNGC	3 cut(s)	136, 1243, 1499
NcoI	CCATGG	1 cut(s)	1522
NdeII	GATC	3 cut(s)	33, 265, 365
NheI	GCTAGC	1 cut(s)	1264
NlaIII	CATG	8 cut(s)	241, 260, 575, 728, 1211, 1241, 1327, 1526
NmuCI	GTSAC	1 cut(s)	85
NsiI	ATGCAT	1 cut(s)	1325
NspI	RCATGY	1 cut(s)	1241
PacI	TTAATTAA	1 cut(s)	1008
PaeI	GCATGC	1 cut(s)	1241
PaeR7I	CTCGAG	1 cut(s)	744
PagI	TCATGA	1 cut(s)	256
PciSI	GCTCTTC	2 cut(s)	318, 710
PfeI	GAWTC	7 cut(s)	215, 230, 433, 547, 1340, 1353, 1535
PkrI	GCNGC	4 cut(s)	107, 126, 129, 1492
PleI	GAGTC	2 cut(s)	153, 736
PpsI	GAGTC	2 cut(s)	153, 736
PshBI	ATTAAT	3 cut(s)	786, 996, 1101
PsiI	TTATAA	1 cut(s)	977
PspEI	GGTNACC	1 cut(s)	1171
PspFI	CCCAGC	1 cut(s)	120
PspPI	GGNCC	3 cut(s)	288, 1255, 1386
PspXI	VCTCGAGB	1 cut(s)	744
RsaI	GTAC	2 cut(s)	583, 1114
RsaNI	GTAC	2 cut(s)	582, 1113
SapI	GCTCTTC	2 cut(s)	318, 710
SaqAI	TTAA	9 cut(s)	77, 345, 566, 786, 996, 1004, 1008, 1101, 1373
SatI	GCNGC	4 cut(s)	106, 125, 128, 1491
Sau3AI	GATC	3 cut(s)	33, 265, 365
Sau96I	GGNCC	3 cut(s)	288, 1255, 1386
SchI	GAGTC	2 cut(s)	154, 736
SduI	GDGCHC	2 cut(s)	834, 993
SfaNI	GCATC	2 cut(s)	808, 1251
Sfr274I	CTCGAG	1 cut(s)	744
SinI	GGWCC	2 cut(s)	288, 1255
SlaI	CTCGAG	1 cut(s)	744
SmlI	CTYRAG	4 cut(s)	710, 744, 1139, 1568
SmoI	CTYRAG	4 cut(s)	710, 744, 1139, 1568
SphI	GCATGC	1 cut(s)	1241
SsiI	CCGC	4 cut(s)	45, 106, 125, 128
SspI	AATATT	1 cut(s)	1126
SspMI	CTAG	1 cut(s)	1265
StyI	CCWWGG	1 cut(s)	1522
TaaI	ACNGT	2 cut(s)	361, 581
TaqI	TCGA	4 cut(s)	364, 745, 1163, 1548
TatI	WGTACW	1 cut(s)	1112
TauI	GCSGC	3 cut(s)	108, 127, 130
TfiI	GAWTC	7 cut(s)	215, 230, 433, 547, 1340, 1353, 1535
Tru1I	TTAA	9 cut(s)	77, 345, 566, 786, 996, 1004, 1008, 1101, 1373
Tru9I	TTAA	9 cut(s)	77, 345, 566, 786, 996, 1004, 1008, 1101, 1373
TscAI	CASTG	2 cut(s)	1276, 1396
TseFI	GTSAC	1 cut(s)	85
TseI	GCWGC	1 cut(s)	1490
Tsp45I	GTSAC	1 cut(s)	85
TspDTI	ATGAA	6 cut(s)	273, 305, 314, 374, 560, 1340
TspGWI	ACGGA	1 cut(s)	645
TspRI	CASTG	2 cut(s)	1276, 1396
VpaK11BI	GGWCC	2 cut(s)	288, 1255
VspI	ATTAAT	3 cut(s)	786, 996, 1101
XapI	RAATTY	5 cut(s)	260, 595, 1195, 1328, 1441
XceI	RCATGY	1 cut(s)	1241
XhoI	CTCGAG	1 cut(s)	744
XspI	CTAG	1 cut(s)	1265
Zsp2I	ATGCAT	1 cut(s)	1325

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.