FvH4_4g22930

Sterile alpha motif.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003205
Physical Location & Seq
Forward (+)
25569945 .. 25572343
2399 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g22930.t1

Sequence Viewer

Length: 855 bp
ATGGCAGCTGAGCTACAGCCACTGGAAGCCACAATCCCCGCCGGCCCAACCACCACCGCCACGTCGACACCCCCACCTCATATACCCACACCTGCCACCGCACCAATCACATCCACAACCGCACCACCACCACCGCCAACGACGGCTCTACACTCGGCTGAGAACCCCGCCCCTCAATTCCAACCGGGCCAGAAACGCCAGCGCCGGCCCAGCGTCCGCCTAGGCGAGATCGGCGACCAGCCCGCTGCCACCGTCTCAATCGACTCGCACATGCGGCGGGCCAGGTACCCCTGGAAGCTCCCGAAGGACTCGAGTAAATCCGTCAAGGCCAGGTCGATCACGAACCTGGTCAACGGCCACGACTCGACGGAGAACGGATTCGAGTTCGGGAACCGGAAGATCAAATCGAAGCGGGCGGCGACGACGAAGCGAGTCCGGTCGAATTGGATAGCGAACTCGAAGATCGACGAGGGAGGGGATAGCCGGGAGGAAGGGGAGGGGTGGATGAGCGCGGATCCGAGTCCGGTGAGGGACGAGAGTCCGGTGCAGTCGGCGGAGGAGAACGATAATTGGGAGGCGGAGGAGCAGAGGAGGGTTTGGGGGAGGGGATCGGAGAGCAGGGAAAACGACGCCGTTGAGATGCCGGAGTCGAATTGGAGCGGGGTGAGGACGTGGCTAATTGAATTGGGGTTGAGTCGGTATACGCCGGTGTTTGAGATACATGAGGTGGATGACGAGGTGTTGCCGATGCTGACGTTGGAGGATCTTAAAGATATGGGGATCAACGCCGTCGGATCACGGCGGAAAATGTACAGTGCGATCCAGAAGCTTCGCAAGGGGTTCTCCATCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAM_1 SAM_2

Protein Analysis

285

Amino Acids

31.4

Weight (kDa)

6.27

Isoelectric Point (pI)

66.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAM_1 PF00536 221 - 278 1.7e-13 SAM domain (Sterile alpha motif)
SAM_2 PF07647 222 - 278 3.9e-13 SAM domain (Sterile alpha motif)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 100
Acc36I ACCTGC 1 cut(s) 100
Acc65I GGTACC 1 cut(s) 285
AccB1I GGYRCC 1 cut(s) 285
AccBSI CCGCTC 1 cut(s) 660
AccI GTMKAC 2 cut(s) 65, 701
AccII CGCG 1 cut(s) 512
AclWI GGATC 7 cut(s) 509, 522, 616, 771, 788, 802, 814
AcoI YGGCCR 1 cut(s) 355
AcyI GRCGYC 1 cut(s) 630
AfaI GTAC 2 cut(s) 287, 812
AgsI TTSAA 1 cut(s) 683
AjiI CACGTC 2 cut(s) 63, 672
AjnI CCWGG 4 cut(s) 281, 290, 329, 345
AluBI AGCT 4 cut(s) 8, 13, 298, 829
AluI AGCT 4 cut(s) 8, 13, 298, 829
Alw26I GTCTC 1 cut(s) 259
AlwI GGATC 7 cut(s) 509, 522, 616, 771, 788, 802, 814
AlwNI CAGNNNCTG 1 cut(s) 22
Ama87I CYCGRG 1 cut(s) 310
AoxI GGCC 6 cut(s) 43, 187, 206, 279, 327, 355
ApeKI GCWGC 2 cut(s) 5, 245
Asp700I GAANNNNTTC 1 cut(s) 377
Asp718I GGTACC 1 cut(s) 285
AspA2I CCTAGG 1 cut(s) 220
AspLEI GCGC 2 cut(s) 204, 512
AspS9I GGNCC 4 cut(s) 44, 187, 207, 279
AsuC2I CCSGG 2 cut(s) 186, 485
AsuHPI GGTGA 2 cut(s) 538, 676
AvaI CYCGRG 1 cut(s) 310
AvrII CCTAGG 1 cut(s) 220
BamHI GGATCC 1 cut(s) 514
BanI GGYRCC 1 cut(s) 285
BbvI GCAGC 2 cut(s) 17, 232
BccI CCATC 1 cut(s) 854
BceAI ACGGC 5 cut(s) 159, 370, 617, 773, 815
BciT130I CCWGG 4 cut(s) 283, 292, 331, 347
BcnI CCSGG 2 cut(s) 186, 485
BcoDI GTCTC 1 cut(s) 259
BfaI CTAG 1 cut(s) 221
BfmI CTRYAG 1 cut(s) 14
BfoI RGCGCY 1 cut(s) 205
BfuAI ACCTGC 1 cut(s) 100
BisI GCNGC 4 cut(s) 6, 246, 275, 417
BlnI CCTAGG 1 cut(s) 220
BlpI GCTNAGC 1 cut(s) 9
BlsI GCNGC 4 cut(s) 7, 247, 276, 418
Bme1390I CCNGG 6 cut(s) 186, 283, 292, 331, 347, 485
BmeT110I CYCGRG 1 cut(s) 310
BmgBI CACGTC 2 cut(s) 63, 672
BmgT120I GGNCC 4 cut(s) 44, 187, 207, 279
BmiI GGNNCC 3 cut(s) 287, 392, 516
BmrFI CCNGG 6 cut(s) 186, 283, 292, 331, 347, 485
BmsI GCATC 2 cut(s) 630, 738
BoxI GACNNNNGTC 1 cut(s) 537
Bpu1102I GCTNAGC 1 cut(s) 9
BpuMI CCSGG 2 cut(s) 186, 485
BsaHI GRCGYC 1 cut(s) 630
BsaJI CCNNGG 2 cut(s) 220, 290
BsaWI WCCGGW 4 cut(s) 393, 435, 523, 541
Bse118I RCCGGY 3 cut(s) 41, 204, 706
Bse1I ACTGG 1 cut(s) 27
BseBI CCWGG 4 cut(s) 283, 292, 331, 347
BseDI CCNNGG 2 cut(s) 220, 290
BseGI GGATG 3 cut(s) 110, 510, 736
BseMII CTCAG 1 cut(s) 150
BseNI ACTGG 1 cut(s) 27
BseRI GAGGAG 3 cut(s) 572, 596, 604
BseXI GCAGC 2 cut(s) 17, 232
BseYI CCCAGC 1 cut(s) 209
BsgI GTGCAG 1 cut(s) 566
Bsh1236I CGCG 1 cut(s) 512
Bsh1285I CGRYCG 1 cut(s) 440
BshFI GGCC 6 cut(s) 45, 189, 208, 281, 329, 357
BshNI GGYRCC 1 cut(s) 285
BsiEI CGRYCG 1 cut(s) 440
BsiHKCI CYCGRG 1 cut(s) 310
BslFI GGGAC 1 cut(s) 545
BsmAI GTCTC 1 cut(s) 259
BsmBI CGTCTC 1 cut(s) 259
BsmFI GGGAC 1 cut(s) 545
BsnI GGCC 6 cut(s) 45, 189, 208, 281, 329, 357
BsoBI CYCGRG 1 cut(s) 310
Bsp1407I TGTACA 1 cut(s) 810
Bsp1720I GCTNAGC 1 cut(s) 9
BspANI GGCC 6 cut(s) 45, 189, 208, 281, 329, 357
BspCNI CTCAG 1 cut(s) 151
BspFNI CGCG 1 cut(s) 512
BspLI GGNNCC 3 cut(s) 287, 392, 516
BspMI ACCTGC 1 cut(s) 100
BspPI GGATC 7 cut(s) 509, 522, 616, 771, 788, 802, 814
BspT107I GGYRCC 1 cut(s) 285
BsrBI CCGCTC 1 cut(s) 660
BsrFI RCCGGY 3 cut(s) 41, 204, 706
BsrGI TGTACA 1 cut(s) 810
BsrI ACTGG 1 cut(s) 27
BssAI RCCGGY 3 cut(s) 41, 204, 706
BssECI CCNNGG 2 cut(s) 220, 290
BssNAI GTATAC 1 cut(s) 702
BssNI GRCGYC 1 cut(s) 630
BssT1I CCWWGG 1 cut(s) 220
Bst1107I GTATAC 1 cut(s) 702
Bst2UI CCWGG 4 cut(s) 283, 292, 331, 347
Bst4CI ACNGT 2 cut(s) 253, 815
BstACI GRCGYC 1 cut(s) 630
BstAUI TGTACA 1 cut(s) 810
BstC8I GCNNGC 6 cut(s) 43, 200, 206, 243, 279, 414
BstDEI CTNAG 2 cut(s) 9, 159
BstF5I GGATG 3 cut(s) 110, 510, 736
BstFNI CGCG 1 cut(s) 512
BstH2I RGCGCY 1 cut(s) 205
BstHHI GCGC 2 cut(s) 204, 512
BstMAI GTCTC 1 cut(s) 259
BstMCI CGRYCG 1 cut(s) 440
BstMWI GCNNNNNNNGC 4 cut(s) 195, 210, 231, 274
BstNI CCWGG 4 cut(s) 283, 292, 331, 347
BstNSI RCATGY 1 cut(s) 274
BstPAI GACNNNNGTC 1 cut(s) 537
BstSCI CCNGG 6 cut(s) 184, 281, 290, 329, 345, 483
BstSFI CTRYAG 1 cut(s) 14
BstUI CGCG 1 cut(s) 512
BstV1I GCAGC 2 cut(s) 17, 232
BstX2I RGATCY 2 cut(s) 514, 763
BstYI RGATCY 2 cut(s) 514, 763
BstZ17I GTATAC 1 cut(s) 702
BsuRI GGCC 6 cut(s) 45, 189, 208, 281, 329, 357
BtrI CACGTC 2 cut(s) 63, 672
BtsCI GGATG 3 cut(s) 110, 510, 736
BtsIMutI CAGTG 2 cut(s) 20, 820
BveI ACCTGC 1 cut(s) 100
Cac8I GCNNGC 6 cut(s) 43, 200, 206, 243, 279, 414
CaiI CAGNNNCTG 1 cut(s) 22
CfoI GCGC 2 cut(s) 204, 512
Cfr10I RCCGGY 3 cut(s) 41, 204, 706
Cfr13I GGNCC 4 cut(s) 44, 187, 207, 279
CseI GACGC 2 cut(s) 202, 638
CsiI ACCWGGT 1 cut(s) 345
Csp6I GTAC 2 cut(s) 286, 811
CviAII CATG 2 cut(s) 271, 722
CviQI GTAC 2 cut(s) 286, 811
DdeI CTNAG 2 cut(s) 9, 159
EaeI YGGCCR 1 cut(s) 355
EciI GGCGGA 4 cut(s) 206, 569, 593, 817
Eco130I CCWWGG 1 cut(s) 220
Eco88I CYCGRG 1 cut(s) 310
EcoRII CCWGG 4 cut(s) 281, 290, 329, 345
EcoT14I CCWWGG 1 cut(s) 220
ErhI CCWWGG 1 cut(s) 220
Esp3I CGTCTC 1 cut(s) 259
FaeI CATG 2 cut(s) 274, 725
FaiI YATR 8 cut(s) 81, 83, 272, 702, 723, 776, 851, 853
FaqI GGGAC 1 cut(s) 545
FatI CATG 2 cut(s) 270, 721
FauI CCCGC 6 cut(s) 46, 175, 250, 270, 405, 653
FauNDI CATATG 1 cut(s) 851
FblI GTMKAC 2 cut(s) 65, 701
Fnu4HI GCNGC 4 cut(s) 6, 246, 275, 417
FokI GGATG 3 cut(s) 97, 517, 743
Fsp4HI GCNGC 4 cut(s) 6, 246, 275, 417
FspBI CTAG 1 cut(s) 221
GlaI GCGC 2 cut(s) 203, 511
GluI GCNGC 4 cut(s) 6, 246, 275, 417
GsaI CCCAGC 1 cut(s) 213
HaeII RGCGCY 1 cut(s) 205
HaeIII GGCC 6 cut(s) 45, 189, 208, 281, 329, 357
HgaI GACGC 2 cut(s) 202, 638
HhaI GCGC 2 cut(s) 204, 512
Hin1I GRCGYC 1 cut(s) 630
Hin1II CATG 2 cut(s) 274, 725
Hin6I GCGC 2 cut(s) 202, 510
HinP1I GCGC 2 cut(s) 202, 510
HincII GTYRAC 2 cut(s) 66, 352
HindII GTYRAC 2 cut(s) 66, 352
HindIII AAGCTT 1 cut(s) 827
HinfI GANTC 9 cut(s) 263, 308, 362, 378, 432, 520, 538, 647, 694
HphI GGTGA 2 cut(s) 538, 676
Hpy166II GTNNAC 3 cut(s) 66, 352, 702
Hpy188I TCNGA 3 cut(s) 519, 613, 794
Hpy188III TCNNGA 4 cut(s) 301, 340, 388, 823
Hpy8I GTNNAC 3 cut(s) 66, 352, 702
Hpy99I CGWCG 8 cut(s) 67, 145, 370, 424, 427, 470, 632, 794
HpyAV CCTTC 2 cut(s) 298, 485
HpyCH4III ACNGT 2 cut(s) 253, 815
HpyCH4IV ACGT 3 cut(s) 62, 671, 755
HpyCH4V TGCA 1 cut(s) 547
HpyF10VI GCNNNNNNNGC 4 cut(s) 195, 210, 231, 274
HpyF3I CTNAG 2 cut(s) 9, 159
HpySE526I ACGT 3 cut(s) 62, 671, 755
Hsp92I GRCGYC 1 cut(s) 630
Hsp92II CATG 2 cut(s) 274, 725
HspAI GCGC 2 cut(s) 202, 510
KpnI GGTACC 1 cut(s) 289
KroI GCCGGC 2 cut(s) 41, 204
KroNI GCCGGC 2 cut(s) 43, 206
LmnI GCTCC 3 cut(s) 303, 583, 657
Lsp1109I GCAGC 2 cut(s) 17, 232
LweI GCATC 2 cut(s) 630, 738
MabI ACCWGGT 1 cut(s) 345
MaeI CTAG 1 cut(s) 221
MaeII ACGT 3 cut(s) 62, 671, 755
MbiI CCGCTC 1 cut(s) 660
MboII GAAGA 2 cut(s) 409, 472
MflI RGATCY 2 cut(s) 514, 763
MluCI AATT 6 cut(s) 176, 442, 568, 652, 678, 683
MlyI GAGTC 8 cut(s) 257, 302, 356, 441, 529, 547, 656, 703
MmeI TCCRAC 3 cut(s) 205, 738, 772
MroNI GCCGGC 2 cut(s) 41, 204
MroXI GAANNNNTTC 1 cut(s) 377
MseI TTAA 1 cut(s) 768
MspA1I CMGCKG 2 cut(s) 8, 245
MspR9I CCNGG 6 cut(s) 186, 283, 292, 331, 347, 485
MvaI CCWGG 4 cut(s) 283, 292, 331, 347
MvnI CGCG 1 cut(s) 512
MwoI GCNNNNNNNGC 4 cut(s) 195, 210, 231, 274
NaeI GCCGGC 2 cut(s) 43, 206
NciI CCSGG 2 cut(s) 186, 485
NdeI CATATG 1 cut(s) 851
NgoMIV GCCGGC 2 cut(s) 41, 204
NlaIII CATG 2 cut(s) 274, 725
NlaIV GGNNCC 3 cut(s) 287, 392, 516
NmeAIII GCCGAG 1 cut(s) 134
NspI RCATGY 1 cut(s) 274
PaeR7I CTCGAG 1 cut(s) 310
PaqCI CACCTGC 1 cut(s) 100
PdiI GCCGGC 2 cut(s) 43, 206
PdmI GAANNNNTTC 1 cut(s) 377
PfeI GAWTC 1 cut(s) 378
PkrI GCNGC 4 cut(s) 7, 247, 276, 418
PleI GAGTC 8 cut(s) 257, 302, 356, 440, 528, 546, 655, 702
PpsI GAGTC 8 cut(s) 257, 302, 356, 440, 528, 546, 655, 702
PshAI GACNNNNGTC 1 cut(s) 537
Psp6I CCWGG 4 cut(s) 281, 290, 329, 345
PspFI CCCAGC 1 cut(s) 209
PspGI CCWGG 4 cut(s) 281, 290, 329, 345
PspN4I GGNNCC 3 cut(s) 287, 392, 516
PspPI GGNCC 4 cut(s) 44, 187, 207, 279
PspXI VCTCGAGB 1 cut(s) 310
PstNI CAGNNNCTG 1 cut(s) 22
PsuI RGATCY 2 cut(s) 514, 763
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 2 cut(s) 287, 812
RsaNI GTAC 2 cut(s) 286, 811
SalI GTCGAC 1 cut(s) 64
SaqAI TTAA 1 cut(s) 768
SatI GCNGC 4 cut(s) 6, 246, 275, 417
Sau96I GGNCC 4 cut(s) 44, 187, 207, 279
SchI GAGTC 8 cut(s) 257, 302, 356, 441, 529, 547, 656, 703
ScrFI CCNGG 6 cut(s) 186, 283, 292, 331, 347, 485
SexAI ACCWGGT 1 cut(s) 345
SfaNI GCATC 2 cut(s) 630, 738
SfcI CTRYAG 1 cut(s) 14
Sfr274I CTCGAG 1 cut(s) 310
SgrAI CRCCGGYG 1 cut(s) 706
SlaI CTCGAG 1 cut(s) 310
SmlI CTYRAG 1 cut(s) 310
SmoI CTYRAG 1 cut(s) 310
Sse9I AATT 6 cut(s) 176, 442, 568, 652, 678, 683
SspMI CTAG 1 cut(s) 221
StyD4I CCNGG 6 cut(s) 184, 281, 290, 329, 345, 483
StyI CCWWGG 1 cut(s) 220
TaaI ACNGT 2 cut(s) 253, 815
TaiI ACGT 3 cut(s) 65, 674, 758
TasI AATT 6 cut(s) 176, 442, 568, 652, 678, 683
TatI WGTACW 1 cut(s) 810
TauI GCSGC 2 cut(s) 277, 419
TfiI GAWTC 1 cut(s) 378
Tru1I TTAA 1 cut(s) 768
Tru9I TTAA 1 cut(s) 768
TscAI CASTG 2 cut(s) 27, 820
TseI GCWGC 2 cut(s) 5, 245
TspGWI ACGGA 3 cut(s) 310, 383, 390
TspRI CASTG 2 cut(s) 27, 820
XceI RCATGY 1 cut(s) 274
XhoI CTCGAG 1 cut(s) 310
XmaJI CCTAGG 1 cut(s) 220
XmiI GTMKAC 2 cut(s) 65, 701
XmnI GAANNNNTTC 1 cut(s) 377
XspI CTAG 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.