RchiOBHm_Chr4g0429181

Sterile alpha motif.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0003205
Physical Location & Seq
Forward (+)
54010733 .. 54012097
1365 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39801

Sequence Viewer

Length: 843 bp
ATGGCAGCTGAGTTACAGCCGCTGGAGGCTACAATCCCCGCCGGCCCAACCGCCACCGCCACGTCGACACCGTCACCTCTTATAACCCCAACGGCGACGGCCGCAATAGCAACCACAACAGCAGCAGCAGCAGCAACAGCAACGGCTCTACACTCGGGAGAAAACCCGGGCCCCCAATTCCAACTGGGCCAGAAACGGCAGCGCCGGCCCAGCGTCCGGTTAGGCGAGATCGGCGACCAGCCCGCCGCCACCGTCTCAATCGACTCACACATGCGAAGGGCCAAGTACCCCTGGAAGCTCCCGAAGGACTCTAGTAAATCCGTCAAGGCCAGGTCGATTACGAACCTCGTCAACGGCCACGATTCGACTGAGATAGATGATCGGAACCAGAACGGCGACGGGGGATTCGAGTTCGGGAGCAGGAAGATCAAGTCGAAGCGGGCGGCGACGACGAAGAGAGTCCGATCGAATTGGATTTCGAATTCGAAGATTGACGAGGGAGGGGATAGCCGGGAGGAGGGGGAGGGGTGGATGAGCGCGGATCCCGATAGGGATAGTCCGGTAAGGGATGAGAGCCCGGTGCAGTCGGCGGAGAACGAGGCGCACAATTGGGAGGAGCAGAGGAGGGTTGGGGGGAATAATTGGGGGAGGGGATCGGAGAGCAGGGAAAACGACGCCCTGGGGTTGAGTCGGTATACGCCGGTGTTCGAGATACATGAGGTGGATGATGAGGTGTTGCCGATGCTGACATTGGAGGATCTTAAAGATATGGGGATAAACGCCGTGGGATCCAGGCGGAAAATGTACAGTGCTATCCAGAAGCTTCGCAAGGGGTTTTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAM_1 SAM_2

Protein Analysis

280

Amino Acids

30.47

Weight (kDa)

6.56

Isoelectric Point (pI)

55.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAM_1 PF00536 225 - 276 1.1e-10 SAM domain (Sterile alpha motif)
SAM_2 PF07647 226 - 276 1.3e-10 SAM domain (Sterile alpha motif)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 83
AccI GTMKAC 2 cut(s) 65, 695
AccII CGCG 1 cut(s) 539
AclWI GGATC 6 cut(s) 536, 549, 661, 765, 783, 796
AcoI YGGCCR 2 cut(s) 99, 355
AcsI RAATTY 1 cut(s) 481
AcyI GRCGYC 1 cut(s) 675
AfaI GTAC 2 cut(s) 287, 806
AfiI CCNNNNNNNGG 2 cut(s) 216, 517
AjiI CACGTC 1 cut(s) 63
AjnI CCWGG 4 cut(s) 290, 329, 678, 791
AluBI AGCT 3 cut(s) 8, 298, 823
AluI AGCT 3 cut(s) 8, 298, 823
Alw26I GTCTC 1 cut(s) 259
AlwI GGATC 6 cut(s) 536, 549, 661, 765, 783, 796
AlwNI CAGNNNCTG 1 cut(s) 22
Ama87I CYCGRG 2 cut(s) 154, 166
AoxI GGCC 8 cut(s) 43, 99, 169, 187, 206, 279, 327, 355
ApaI GGGCCC 1 cut(s) 173
ApeKI GCWGC 6 cut(s) 5, 122, 125, 128, 131, 199
ApoI RAATTY 1 cut(s) 481
ArsI GACNNNNNNTTYG 2 cut(s) 389, 421
AspLEI GCGC 3 cut(s) 204, 539, 604
AspS9I GGNCC 6 cut(s) 44, 169, 170, 187, 207, 279
AsuC2I CCSGG 4 cut(s) 167, 168, 512, 578
AsuHPI GGTGA 1 cut(s) 66
AsuII TTCGAA 2 cut(s) 479, 485
AvaI CYCGRG 2 cut(s) 154, 166
BaeGI GKGCMC 1 cut(s) 173
BamHI GGATCC 2 cut(s) 541, 788
BanII GRGCYC 2 cut(s) 173, 578
BbvI GCAGC 6 cut(s) 17, 134, 137, 140, 143, 211
BceAI ACGGC 7 cut(s) 108, 114, 159, 212, 370, 409, 767
BciT130I CCWGG 4 cut(s) 292, 331, 680, 793
BcnI CCSGG 4 cut(s) 167, 168, 512, 578
BcoDI GTCTC 1 cut(s) 259
BfaI CTAG 1 cut(s) 312
BfoI RGCGCY 1 cut(s) 205
Bme1390I CCNGG 8 cut(s) 167, 168, 292, 331, 512, 578, 680, 793
BmeT110I CYCGRG 2 cut(s) 154, 166
BmgBI CACGTC 1 cut(s) 63
BmgT120I GGNCC 6 cut(s) 44, 169, 170, 187, 207, 279
BmiI GGNNCC 5 cut(s) 171, 172, 386, 543, 790
BmrFI CCNGG 8 cut(s) 167, 168, 292, 331, 512, 578, 680, 793
BmrI ACTGGG 1 cut(s) 194
BmsI GCATC 1 cut(s) 732
BmuI ACTGGG 1 cut(s) 194
BpmI CTGGAG 1 cut(s) 44
Bpu14I TTCGAA 2 cut(s) 479, 485
BpuMI CCSGG 4 cut(s) 167, 168, 512, 578
BsaHI GRCGYC 1 cut(s) 675
BsaJI CCNNGG 5 cut(s) 166, 290, 678, 679, 783
BsaWI WCCGGW 2 cut(s) 216, 559
Bsc4I CCNNNNNNNGG 2 cut(s) 216, 517
Bse118I RCCGGY 3 cut(s) 41, 204, 700
Bse1I ACTGG 1 cut(s) 189
BseBI CCWGG 4 cut(s) 292, 331, 680, 793
BseDI CCNNGG 5 cut(s) 166, 290, 678, 679, 783
BseGI GGATG 3 cut(s) 537, 574, 730
BseLI CCNNNNNNNGG 2 cut(s) 216, 517
BseMII CTCAG 1 cut(s) 360
BseNI ACTGG 1 cut(s) 189
BseRI GAGGAG 3 cut(s) 530, 629, 637
BseSI GKGCMC 1 cut(s) 173
BseX3I CGGCCG 1 cut(s) 99
BseXI GCAGC 6 cut(s) 17, 134, 137, 140, 143, 211
BseYI CCCAGC 1 cut(s) 209
BsgI GTGCAG 1 cut(s) 602
Bsh1236I CGCG 1 cut(s) 539
Bsh1285I CGRYCG 2 cut(s) 102, 467
BshFI GGCC 8 cut(s) 45, 101, 171, 189, 208, 281, 329, 357
BsiEI CGRYCG 2 cut(s) 102, 467
BsiHKCI CYCGRG 2 cut(s) 154, 166
BsiSI CCGG 8 cut(s) 42, 167, 205, 217, 511, 560, 578, 701
BslI CCNNNNNNNGG 2 cut(s) 216, 517
BsmAI GTCTC 1 cut(s) 259
BsmBI CGTCTC 1 cut(s) 259
BsnI GGCC 8 cut(s) 45, 101, 171, 189, 208, 281, 329, 357
BsoBI CYCGRG 2 cut(s) 154, 166
Bsp119I TTCGAA 2 cut(s) 479, 485
Bsp120I GGGCCC 1 cut(s) 169
Bsp1286I GDGCHC 2 cut(s) 173, 578
Bsp1407I TGTACA 1 cut(s) 804
Bsp143I GATC 8 cut(s) 228, 379, 426, 464, 541, 653, 757, 788
BspANI GGCC 8 cut(s) 45, 101, 171, 189, 208, 281, 329, 357
BspCNI CTCAG 1 cut(s) 361
BspFNI CGCG 1 cut(s) 539
BspLI GGNNCC 5 cut(s) 171, 172, 386, 543, 790
BspPI GGATC 6 cut(s) 536, 549, 661, 765, 783, 796
BspT104I TTCGAA 2 cut(s) 479, 485
BsrFI RCCGGY 3 cut(s) 41, 204, 700
BsrGI TGTACA 1 cut(s) 804
BsrI ACTGG 1 cut(s) 189
BssAI RCCGGY 3 cut(s) 41, 204, 700
BssECI CCNNGG 5 cut(s) 166, 290, 678, 679, 783
BssMI GATC 8 cut(s) 228, 379, 426, 464, 541, 653, 757, 788
BssNAI GTATAC 1 cut(s) 696
BssNI GRCGYC 1 cut(s) 675
Bst1107I GTATAC 1 cut(s) 696
Bst2UI CCWGG 4 cut(s) 292, 331, 680, 793
Bst4CI ACNGT 3 cut(s) 72, 253, 809
Bst6I CTCTTC 1 cut(s) 449
BstACI GRCGYC 1 cut(s) 675
BstAUI TGTACA 1 cut(s) 804
BstBI TTCGAA 2 cut(s) 479, 485
BstC8I GCNNGC 4 cut(s) 43, 206, 243, 441
BstDEI CTNAG 2 cut(s) 9, 369
BstDSI CCRYGG 1 cut(s) 783
BstF5I GGATG 3 cut(s) 537, 574, 730
BstFNI CGCG 1 cut(s) 539
BstH2I RGCGCY 1 cut(s) 205
BstHHI GCGC 3 cut(s) 204, 539, 604
BstKTI GATC 8 cut(s) 231, 382, 429, 467, 544, 656, 760, 791
BstMAI GTCTC 1 cut(s) 259
BstMBI GATC 8 cut(s) 228, 379, 426, 464, 541, 653, 757, 788
BstMCI CGRYCG 2 cut(s) 102, 467
BstMWI GCNNNNNNNGC 8 cut(s) 101, 107, 128, 131, 137, 205, 210, 231
BstNI CCWGG 4 cut(s) 292, 331, 680, 793
BstNSI RCATGY 1 cut(s) 274
BstSCI CCNGG 8 cut(s) 165, 166, 290, 329, 510, 576, 678, 791
BstSLI GKGCMC 1 cut(s) 173
BstUI CGCG 1 cut(s) 539
BstV1I GCAGC 6 cut(s) 17, 134, 137, 140, 143, 211
BstX2I RGATCY 3 cut(s) 541, 757, 788
BstYI RGATCY 3 cut(s) 541, 757, 788
BstZ17I GTATAC 1 cut(s) 696
BstZI CGGCCG 1 cut(s) 99
BsuRI GGCC 8 cut(s) 45, 101, 171, 189, 208, 281, 329, 357
BtgI CCRYGG 1 cut(s) 783
BtrI CACGTC 1 cut(s) 63
BtsCI GGATG 3 cut(s) 537, 574, 730
BtsIMutI CAGTG 1 cut(s) 814
Cac8I GCNNGC 4 cut(s) 43, 206, 243, 441
CaiI CAGNNNCTG 1 cut(s) 22
CfoI GCGC 3 cut(s) 204, 539, 604
Cfr10I RCCGGY 3 cut(s) 41, 204, 700
Cfr13I GGNCC 6 cut(s) 44, 169, 170, 187, 207, 279
Cfr9I CCCGGG 1 cut(s) 166
CseI GACGC 2 cut(s) 202, 683
Csp6I GTAC 2 cut(s) 286, 805
CviAII CATG 2 cut(s) 271, 716
CviQI GTAC 2 cut(s) 286, 805
DdeI CTNAG 2 cut(s) 9, 369
DpnI GATC 8 cut(s) 230, 381, 428, 466, 543, 655, 759, 790
DpnII GATC 8 cut(s) 228, 379, 426, 464, 541, 653, 757, 788
EaeI YGGCCR 2 cut(s) 99, 355
EagI CGGCCG 1 cut(s) 99
Eam1104I CTCTTC 1 cut(s) 449
EarI CTCTTC 1 cut(s) 449
EciI GGCGGA 2 cut(s) 605, 811
EclXI CGGCCG 1 cut(s) 99
Eco24I GRGCYC 2 cut(s) 173, 578
Eco52I CGGCCG 1 cut(s) 99
Eco88I CYCGRG 2 cut(s) 154, 166
EcoO109I RGGNCCY 1 cut(s) 170
EcoRI GAATTC 1 cut(s) 481
EcoRII CCWGG 4 cut(s) 290, 329, 678, 791
EcoT38I GRGCYC 2 cut(s) 173, 578
Esp3I CGTCTC 1 cut(s) 259
FaeI CATG 2 cut(s) 274, 719
FaiI YATR 5 cut(s) 83, 272, 696, 717, 770
FatI CATG 2 cut(s) 270, 715
FauI CCCGC 3 cut(s) 46, 250, 432
FblI GTMKAC 2 cut(s) 65, 695
FokI GGATG 3 cut(s) 544, 581, 737
FriOI GRGCYC 2 cut(s) 173, 578
FspBI CTAG 1 cut(s) 312
GlaI GCGC 3 cut(s) 203, 538, 603
GsaI CCCAGC 1 cut(s) 213
GsuI CTGGAG 1 cut(s) 44
HaeII RGCGCY 1 cut(s) 205
HaeIII GGCC 8 cut(s) 45, 101, 171, 189, 208, 281, 329, 357
HapII CCGG 8 cut(s) 42, 167, 205, 217, 511, 560, 578, 701
HgaI GACGC 2 cut(s) 202, 683
HhaI GCGC 3 cut(s) 204, 539, 604
Hin1I GRCGYC 1 cut(s) 675
Hin1II CATG 2 cut(s) 274, 719
Hin6I GCGC 3 cut(s) 202, 537, 602
HinP1I GCGC 3 cut(s) 202, 537, 602
HincII GTYRAC 2 cut(s) 66, 352
HindII GTYRAC 2 cut(s) 66, 352
HindIII AAGCTT 1 cut(s) 821
HinfI GANTC 6 cut(s) 263, 308, 362, 405, 459, 688
HpaII CCGG 8 cut(s) 42, 167, 205, 217, 511, 560, 578, 701
HphI GGTGA 1 cut(s) 66
Hpy166II GTNNAC 3 cut(s) 66, 352, 696
Hpy188I TCNGA 3 cut(s) 384, 464, 658
Hpy188III TCNNGA 7 cut(s) 156, 301, 415, 545, 709, 817, 840
Hpy8I GTNNAC 3 cut(s) 66, 352, 696
Hpy99I CGWCG 6 cut(s) 67, 100, 401, 451, 454, 677
HpyAV CCTTC 2 cut(s) 270, 298
HpyCH4III ACNGT 3 cut(s) 72, 253, 809
HpyCH4IV ACGT 1 cut(s) 62
HpyCH4V TGCA 1 cut(s) 583
HpyF10VI GCNNNNNNNGC 8 cut(s) 101, 107, 128, 131, 137, 205, 210, 231
HpyF3I CTNAG 2 cut(s) 9, 369
HpySE526I ACGT 1 cut(s) 62
Hsp92I GRCGYC 1 cut(s) 675
Hsp92II CATG 2 cut(s) 274, 719
HspAI GCGC 3 cut(s) 202, 537, 602
KroI GCCGGC 2 cut(s) 41, 204
KroNI GCCGGC 2 cut(s) 43, 206
Kzo9I GATC 8 cut(s) 228, 379, 426, 464, 541, 653, 757, 788
LmnI GCTCC 3 cut(s) 303, 417, 616
Lsp1109I GCAGC 6 cut(s) 17, 134, 137, 140, 143, 211
LweI GCATC 1 cut(s) 732
MaeI CTAG 1 cut(s) 312
MaeII ACGT 1 cut(s) 62
MaeIII GTNAC 2 cut(s) 12, 72
MalI GATC 8 cut(s) 230, 381, 428, 466, 543, 655, 759, 790
MboI GATC 8 cut(s) 228, 379, 426, 464, 541, 653, 757, 788
MboII GAAGA 3 cut(s) 436, 466, 499
MfeI CAATTG 1 cut(s) 607
MflI RGATCY 3 cut(s) 541, 757, 788
MhlI GDGCHC 2 cut(s) 173, 578
MluCI AATT 5 cut(s) 176, 469, 481, 607, 640
MlyI GAGTC 4 cut(s) 257, 302, 468, 697
MmeI TCCRAC 1 cut(s) 205
MroNI GCCGGC 2 cut(s) 41, 204
MseI TTAA 1 cut(s) 762
MspA1I CMGCKG 2 cut(s) 8, 22
MspI CCGG 8 cut(s) 42, 167, 205, 217, 511, 560, 578, 701
MspR9I CCNGG 8 cut(s) 167, 168, 292, 331, 512, 578, 680, 793
MunI CAATTG 1 cut(s) 607
MvaI CCWGG 4 cut(s) 292, 331, 680, 793
MvnI CGCG 1 cut(s) 539
MwoI GCNNNNNNNGC 8 cut(s) 101, 107, 128, 131, 137, 205, 210, 231
NaeI GCCGGC 2 cut(s) 43, 206
NciI CCSGG 4 cut(s) 167, 168, 512, 578
NdeII GATC 8 cut(s) 228, 379, 426, 464, 541, 653, 757, 788
NgoMIV GCCGGC 2 cut(s) 41, 204
NlaIII CATG 2 cut(s) 274, 719
NlaIV GGNNCC 5 cut(s) 171, 172, 386, 543, 790
NmuCI GTSAC 1 cut(s) 72
NspI RCATGY 1 cut(s) 274
NspV TTCGAA 2 cut(s) 479, 485
PasI CCCWGGG 1 cut(s) 679
PcsI WCGNNNNNNNCGW 2 cut(s) 68, 405
PdiI GCCGGC 2 cut(s) 43, 206
PfeI GAWTC 2 cut(s) 362, 405
PflFI GACNNNGTC 1 cut(s) 70
Ple19I CGATCG 1 cut(s) 467
PleI GAGTC 4 cut(s) 257, 302, 467, 696
PpsI GAGTC 4 cut(s) 257, 302, 467, 696
PsiI TTATAA 1 cut(s) 83
Psp6I CCWGG 4 cut(s) 290, 329, 678, 791
PspFI CCCAGC 1 cut(s) 209
PspGI CCWGG 4 cut(s) 290, 329, 678, 791
PspN4I GGNNCC 5 cut(s) 171, 172, 386, 543, 790
PspOMI GGGCCC 1 cut(s) 169
PspPI GGNCC 6 cut(s) 44, 169, 170, 187, 207, 279
PstNI CAGNNNCTG 1 cut(s) 22
PsuI RGATCY 3 cut(s) 541, 757, 788
PsyI GACNNNGTC 1 cut(s) 70
PvuI CGATCG 1 cut(s) 467
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 2 cut(s) 287, 806
RsaNI GTAC 2 cut(s) 286, 805
SalI GTCGAC 1 cut(s) 64
SaqAI TTAA 1 cut(s) 762
Sau3AI GATC 8 cut(s) 228, 379, 426, 464, 541, 653, 757, 788
Sau96I GGNCC 6 cut(s) 44, 169, 170, 187, 207, 279
SchI GAGTC 4 cut(s) 257, 302, 468, 697
ScrFI CCNGG 8 cut(s) 167, 168, 292, 331, 512, 578, 680, 793
SduI GDGCHC 2 cut(s) 173, 578
SetI ASST 9 cut(s) 10, 65, 79, 300, 335, 348, 723, 735, 825
SfaNI GCATC 1 cut(s) 732
SfuI TTCGAA 2 cut(s) 479, 485
SgrAI CRCCGGYG 1 cut(s) 700
SmaI CCCGGG 1 cut(s) 168
Sse9I AATT 5 cut(s) 176, 469, 481, 607, 640
SspMI CTAG 1 cut(s) 312
StyD4I CCNGG 8 cut(s) 165, 166, 290, 329, 510, 576, 678, 791
TaaI ACNGT 3 cut(s) 72, 253, 809
TaiI ACGT 1 cut(s) 65
TasI AATT 5 cut(s) 176, 469, 481, 607, 640
TatI WGTACW 1 cut(s) 804
TauI GCSGC 4 cut(s) 22, 104, 248, 446
TfiI GAWTC 2 cut(s) 362, 405
Tru1I TTAA 1 cut(s) 762
Tru9I TTAA 1 cut(s) 762
TscAI CASTG 1 cut(s) 814
TseFI GTSAC 1 cut(s) 72
TseI GCWGC 6 cut(s) 5, 122, 125, 128, 131, 199
Tsp45I GTSAC 1 cut(s) 72
TspGWI ACGGA 1 cut(s) 310
TspMI CCCGGG 1 cut(s) 166
TspRI CASTG 1 cut(s) 814
Tth111I GACNNNGTC 1 cut(s) 70
XapI RAATTY 1 cut(s) 481
XceI RCATGY 1 cut(s) 274
XmaI CCCGGG 1 cut(s) 166
XmiI GTMKAC 2 cut(s) 65, 695
XspI CTAG 1 cut(s) 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.