Rh4AG289600

Sterile alpha motif.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0003205
Physical Location & Seq
Forward (+)
60307700 .. 60310589
2890 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG289600.1

Sequence Viewer

Length: 912 bp
ATGGCAGCTGAGTTACAGCCGCTGGAGGCTACAATCCCCGCCGGCCCAACCGCCACCGCCACGTCGACACCGTCACCTCTTATAACCCCAACGGCGACGGCCGCAATAGCAACCACAACAGCAGCAGCAGCAGCAGCAACAGCAACAGCAACGGCTCTACACTCGGGCGAAAACCCGGGCCCCCAATTCCAACTGGGCCAGAAACGGCAGCGCCGGCCCAGCGTCCGGTTAGGCGAGATCGGCGACCAGCCCGCCGCCACCGTCTCAATCGACTCACACATGCGAAGGGCCAAGTACCCCTGGAAGCTCCCGAAGGACTCTAGTAAATCCGTCAAGGCCAGGTCGATTACGAACCTCGTCAACGGCCACGATTCGACTGAGATAGATGATCGGAACCAGAACGGCGACGGGGGATTCGAGTTCGGGAGCAGGAAGATCAAATCGAAGCGGGCGGCGACGACGAAGAGAGTCCGATCGAATTGGATTTCGAATTCGAAGATTGACGAGGGAGGGGATAGCCGGGAGGATGGGGAGGGGTGGATGAGCGCAGATCCCGATAGGGATAGTCCGGTGAGGGATGAGAGTCCGGTGCAGTCGGCGGAGAACGAGGCGCACAATTGGGAGGAGCAGAGGAGGGTTGGGGGGAATAATTGGGGGAGGGGATCGGAGAGCAGGGAAAACGACGCCGTTGAGGCGGATAATATGCCGGAGTCGAATTGGAGCGGAGTGAGGTCGTGGCTAATAGAGCTGGGGCTGAGTCGGTATACGCCGGTGTTCGAGATACATGAGGTGGATGATGAGGTGTTGCCGATGCTGACATTGGAGGATCTTAAAGATATGGGGATAAACGCCGTCGGATCCAGGCGGAAAATGTACAGTGCTATCCAGAAGCTTCGCAAGGGGTTTTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAM_1 SAM_2

Protein Analysis

303

Amino Acids

33.0

Weight (kDa)

5.85

Isoelectric Point (pI)

57.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAM_1 PF00536 242 - 299 2.2e-13 SAM domain (Sterile alpha motif)
SAM_2 PF07647 243 - 299 1.3e-13 SAM domain (Sterile alpha motif)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 83
AccBSI CCGCTC 1 cut(s) 723
AccI GTMKAC 2 cut(s) 65, 764
AclWI GGATC 5 cut(s) 545, 670, 834, 852, 865
AcoI YGGCCR 2 cut(s) 99, 364
AcsI RAATTY 1 cut(s) 490
AcyI GRCGYC 1 cut(s) 684
AfaI GTAC 2 cut(s) 296, 875
AfiI CCNNNNNNNGG 1 cut(s) 225
AjiI CACGTC 1 cut(s) 63
AjnI CCWGG 3 cut(s) 299, 338, 860
AluBI AGCT 4 cut(s) 8, 307, 748, 892
AluI AGCT 4 cut(s) 8, 307, 748, 892
Alw26I GTCTC 1 cut(s) 268
AlwI GGATC 5 cut(s) 545, 670, 834, 852, 865
AlwNI CAGNNNCTG 1 cut(s) 22
Ama87I CYCGRG 2 cut(s) 163, 175
AoxI GGCC 8 cut(s) 43, 99, 178, 196, 215, 288, 336, 364
ApaI GGGCCC 1 cut(s) 182
ApeKI GCWGC 7 cut(s) 5, 122, 125, 128, 131, 134, 208
ApoI RAATTY 1 cut(s) 490
ArsI GACNNNNNNTTYG 2 cut(s) 398, 430
AspLEI GCGC 3 cut(s) 213, 548, 613
AspS9I GGNCC 6 cut(s) 44, 178, 179, 196, 216, 288
AsuC2I CCSGG 3 cut(s) 176, 177, 521
AsuHPI GGTGA 2 cut(s) 66, 583
AsuII TTCGAA 2 cut(s) 488, 494
AvaI CYCGRG 2 cut(s) 163, 175
BaeGI GKGCMC 1 cut(s) 182
BamHI GGATCC 1 cut(s) 857
BanII GRGCYC 1 cut(s) 182
BbvI GCAGC 7 cut(s) 17, 134, 137, 140, 143, 146, 220
BccI CCATC 1 cut(s) 521
BceAI ACGGC 8 cut(s) 108, 114, 168, 221, 379, 418, 671, 836
BciT130I CCWGG 3 cut(s) 301, 340, 862
BcnI CCSGG 3 cut(s) 176, 177, 521
BcoDI GTCTC 1 cut(s) 268
BfaI CTAG 1 cut(s) 321
BfoI RGCGCY 1 cut(s) 214
BglI GCCNNNNNGGC 1 cut(s) 692
Bme1390I CCNGG 6 cut(s) 176, 177, 301, 340, 521, 862
BmeT110I CYCGRG 2 cut(s) 163, 175
BmgBI CACGTC 1 cut(s) 63
BmgT120I GGNCC 6 cut(s) 44, 178, 179, 196, 216, 288
BmiI GGNNCC 4 cut(s) 180, 181, 395, 859
BmrFI CCNGG 6 cut(s) 176, 177, 301, 340, 521, 862
BmrI ACTGGG 1 cut(s) 203
BmsI GCATC 1 cut(s) 801
BmuI ACTGGG 1 cut(s) 203
BpmI CTGGAG 1 cut(s) 44
Bpu14I TTCGAA 2 cut(s) 488, 494
BpuMI CCSGG 3 cut(s) 176, 177, 521
BsaHI GRCGYC 1 cut(s) 684
BsaJI CCNNGG 2 cut(s) 175, 299
BsaWI WCCGGW 3 cut(s) 225, 568, 586
Bsc4I CCNNNNNNNGG 1 cut(s) 225
Bse118I RCCGGY 3 cut(s) 41, 213, 769
Bse1I ACTGG 1 cut(s) 198
BseBI CCWGG 3 cut(s) 301, 340, 862
BseDI CCNNGG 2 cut(s) 175, 299
BseGI GGATG 4 cut(s) 532, 546, 583, 799
BseLI CCNNNNNNNGG 1 cut(s) 225
BseMII CTCAG 2 cut(s) 369, 746
BseNI ACTGG 1 cut(s) 198
BseRI GAGGAG 2 cut(s) 638, 646
BseSI GKGCMC 1 cut(s) 182
BseX3I CGGCCG 1 cut(s) 99
BseXI GCAGC 7 cut(s) 17, 134, 137, 140, 143, 146, 220
BseYI CCCAGC 2 cut(s) 218, 748
BsgI GTGCAG 1 cut(s) 611
Bsh1285I CGRYCG 2 cut(s) 102, 476
BshFI GGCC 8 cut(s) 45, 101, 180, 198, 217, 290, 338, 366
BsiEI CGRYCG 2 cut(s) 102, 476
BsiHKCI CYCGRG 2 cut(s) 163, 175
BsiSI CCGG 9 cut(s) 42, 176, 214, 226, 520, 569, 587, 707, 770
BslI CCNNNNNNNGG 1 cut(s) 225
BsmAI GTCTC 1 cut(s) 268
BsmBI CGTCTC 1 cut(s) 268
BsnI GGCC 8 cut(s) 45, 101, 180, 198, 217, 290, 338, 366
BsoBI CYCGRG 2 cut(s) 163, 175
Bsp119I TTCGAA 2 cut(s) 488, 494
Bsp120I GGGCCC 1 cut(s) 178
Bsp1286I GDGCHC 1 cut(s) 182
Bsp1407I TGTACA 1 cut(s) 873
Bsp143I GATC 8 cut(s) 237, 388, 435, 473, 550, 662, 826, 857
BspANI GGCC 8 cut(s) 45, 101, 180, 198, 217, 290, 338, 366
BspCNI CTCAG 2 cut(s) 370, 747
BspLI GGNNCC 4 cut(s) 180, 181, 395, 859
BspPI GGATC 5 cut(s) 545, 670, 834, 852, 865
BspT104I TTCGAA 2 cut(s) 488, 494
BsrBI CCGCTC 1 cut(s) 723
BsrFI RCCGGY 3 cut(s) 41, 213, 769
BsrGI TGTACA 1 cut(s) 873
BsrI ACTGG 1 cut(s) 198
BssAI RCCGGY 3 cut(s) 41, 213, 769
BssECI CCNNGG 2 cut(s) 175, 299
BssMI GATC 8 cut(s) 237, 388, 435, 473, 550, 662, 826, 857
BssNAI GTATAC 1 cut(s) 765
BssNI GRCGYC 1 cut(s) 684
Bst1107I GTATAC 1 cut(s) 765
Bst2UI CCWGG 3 cut(s) 301, 340, 862
Bst4CI ACNGT 3 cut(s) 72, 262, 878
Bst6I CTCTTC 1 cut(s) 458
BstACI GRCGYC 1 cut(s) 684
BstAUI TGTACA 1 cut(s) 873
BstBI TTCGAA 2 cut(s) 488, 494
BstC8I GCNNGC 4 cut(s) 43, 215, 252, 450
BstDEI CTNAG 3 cut(s) 9, 378, 755
BstF5I GGATG 4 cut(s) 532, 546, 583, 799
BstH2I RGCGCY 1 cut(s) 214
BstHHI GCGC 3 cut(s) 213, 548, 613
BstKTI GATC 8 cut(s) 240, 391, 438, 476, 553, 665, 829, 860
BstMAI GTCTC 1 cut(s) 268
BstMBI GATC 8 cut(s) 237, 388, 435, 473, 550, 662, 826, 857
BstMCI CGRYCG 2 cut(s) 102, 476
BstNI CCWGG 3 cut(s) 301, 340, 862
BstNSI RCATGY 1 cut(s) 283
BstSCI CCNGG 6 cut(s) 174, 175, 299, 338, 519, 860
BstSLI GKGCMC 1 cut(s) 182
BstV1I GCAGC 7 cut(s) 17, 134, 137, 140, 143, 146, 220
BstX2I RGATCY 3 cut(s) 550, 826, 857
BstYI RGATCY 3 cut(s) 550, 826, 857
BstZ17I GTATAC 1 cut(s) 765
BstZI CGGCCG 1 cut(s) 99
BsuRI GGCC 8 cut(s) 45, 101, 180, 198, 217, 290, 338, 366
BtrI CACGTC 1 cut(s) 63
BtsCI GGATG 4 cut(s) 532, 546, 583, 799
BtsIMutI CAGTG 1 cut(s) 883
Cac8I GCNNGC 4 cut(s) 43, 215, 252, 450
CaiI CAGNNNCTG 1 cut(s) 22
CfoI GCGC 3 cut(s) 213, 548, 613
Cfr10I RCCGGY 3 cut(s) 41, 213, 769
Cfr13I GGNCC 6 cut(s) 44, 178, 179, 196, 216, 288
Cfr9I CCCGGG 1 cut(s) 175
CseI GACGC 2 cut(s) 211, 692
Csp6I GTAC 2 cut(s) 295, 874
CviAII CATG 2 cut(s) 280, 785
CviQI GTAC 2 cut(s) 295, 874
DdeI CTNAG 3 cut(s) 9, 378, 755
DpnI GATC 8 cut(s) 239, 390, 437, 475, 552, 664, 828, 859
DpnII GATC 8 cut(s) 237, 388, 435, 473, 550, 662, 826, 857
EaeI YGGCCR 2 cut(s) 99, 364
EagI CGGCCG 1 cut(s) 99
Eam1104I CTCTTC 1 cut(s) 458
EarI CTCTTC 1 cut(s) 458
EciI GGCGGA 3 cut(s) 614, 710, 880
EclXI CGGCCG 1 cut(s) 99
Eco24I GRGCYC 1 cut(s) 182
Eco52I CGGCCG 1 cut(s) 99
Eco88I CYCGRG 2 cut(s) 163, 175
EcoO109I RGGNCCY 1 cut(s) 179
EcoRI GAATTC 1 cut(s) 490
EcoRII CCWGG 3 cut(s) 299, 338, 860
EcoT38I GRGCYC 1 cut(s) 182
Esp3I CGTCTC 1 cut(s) 268
FaeI CATG 2 cut(s) 283, 788
FaiI YATR 6 cut(s) 83, 281, 704, 765, 786, 839
FatI CATG 2 cut(s) 279, 784
FauI CCCGC 3 cut(s) 46, 259, 441
FblI GTMKAC 2 cut(s) 65, 764
FokI GGATG 4 cut(s) 539, 553, 590, 806
FriOI GRGCYC 1 cut(s) 182
FspBI CTAG 1 cut(s) 321
GlaI GCGC 3 cut(s) 212, 547, 612
GsaI CCCAGC 2 cut(s) 222, 752
GsuI CTGGAG 1 cut(s) 44
HaeII RGCGCY 1 cut(s) 214
HaeIII GGCC 8 cut(s) 45, 101, 180, 198, 217, 290, 338, 366
HapII CCGG 9 cut(s) 42, 176, 214, 226, 520, 569, 587, 707, 770
HgaI GACGC 2 cut(s) 211, 692
HhaI GCGC 3 cut(s) 213, 548, 613
Hin1I GRCGYC 1 cut(s) 684
Hin1II CATG 2 cut(s) 283, 788
Hin6I GCGC 3 cut(s) 211, 546, 611
HinP1I GCGC 3 cut(s) 211, 546, 611
HincII GTYRAC 2 cut(s) 66, 361
HindII GTYRAC 2 cut(s) 66, 361
HindIII AAGCTT 1 cut(s) 890
HinfI GANTC 8 cut(s) 272, 317, 371, 414, 468, 583, 710, 757
HpaII CCGG 9 cut(s) 42, 176, 214, 226, 520, 569, 587, 707, 770
HphI GGTGA 2 cut(s) 66, 583
Hpy166II GTNNAC 3 cut(s) 66, 361, 765
Hpy188I TCNGA 4 cut(s) 393, 473, 667, 857
Hpy188III TCNNGA 6 cut(s) 310, 424, 554, 778, 886, 909
Hpy8I GTNNAC 3 cut(s) 66, 361, 765
Hpy99I CGWCG 7 cut(s) 67, 100, 410, 460, 463, 686, 857
HpyAV CCTTC 2 cut(s) 279, 307
HpyCH4III ACNGT 3 cut(s) 72, 262, 878
HpyCH4IV ACGT 1 cut(s) 62
HpyCH4V TGCA 1 cut(s) 592
HpyF3I CTNAG 3 cut(s) 9, 378, 755
HpySE526I ACGT 1 cut(s) 62
Hsp92I GRCGYC 1 cut(s) 684
Hsp92II CATG 2 cut(s) 283, 788
HspAI GCGC 3 cut(s) 211, 546, 611
KroI GCCGGC 2 cut(s) 41, 213
KroNI GCCGGC 2 cut(s) 43, 215
Kzo9I GATC 8 cut(s) 237, 388, 435, 473, 550, 662, 826, 857
LmnI GCTCC 4 cut(s) 312, 426, 625, 720
Lsp1109I GCAGC 7 cut(s) 17, 134, 137, 140, 143, 146, 220
LweI GCATC 1 cut(s) 801
MaeI CTAG 1 cut(s) 321
MaeII ACGT 1 cut(s) 62
MaeIII GTNAC 2 cut(s) 12, 72
MalI GATC 8 cut(s) 239, 390, 437, 475, 552, 664, 828, 859
MbiI CCGCTC 1 cut(s) 723
MboI GATC 8 cut(s) 237, 388, 435, 473, 550, 662, 826, 857
MboII GAAGA 3 cut(s) 445, 475, 508
MfeI CAATTG 1 cut(s) 616
MflI RGATCY 3 cut(s) 550, 826, 857
MhlI GDGCHC 1 cut(s) 182
MluCI AATT 6 cut(s) 185, 478, 490, 616, 649, 715
MlyI GAGTC 6 cut(s) 266, 311, 477, 592, 719, 766
MmeI TCCRAC 2 cut(s) 214, 835
MroNI GCCGGC 2 cut(s) 41, 213
MseI TTAA 1 cut(s) 831
MspA1I CMGCKG 2 cut(s) 8, 22
MspI CCGG 9 cut(s) 42, 176, 214, 226, 520, 569, 587, 707, 770
MspR9I CCNGG 6 cut(s) 176, 177, 301, 340, 521, 862
MunI CAATTG 1 cut(s) 616
MvaI CCWGG 3 cut(s) 301, 340, 862
NaeI GCCGGC 2 cut(s) 43, 215
NciI CCSGG 3 cut(s) 176, 177, 521
NdeII GATC 8 cut(s) 237, 388, 435, 473, 550, 662, 826, 857
NgoMIV GCCGGC 2 cut(s) 41, 213
NlaIII CATG 2 cut(s) 283, 788
NlaIV GGNNCC 4 cut(s) 180, 181, 395, 859
NmuCI GTSAC 1 cut(s) 72
NspI RCATGY 1 cut(s) 283
NspV TTCGAA 2 cut(s) 488, 494
PcsI WCGNNNNNNNCGW 2 cut(s) 68, 414
PdiI GCCGGC 2 cut(s) 43, 215
PfeI GAWTC 2 cut(s) 371, 414
PflFI GACNNNGTC 1 cut(s) 70
Ple19I CGATCG 1 cut(s) 476
PleI GAGTC 6 cut(s) 266, 311, 476, 591, 718, 765
PpsI GAGTC 6 cut(s) 266, 311, 476, 591, 718, 765
PsiI TTATAA 1 cut(s) 83
Psp6I CCWGG 3 cut(s) 299, 338, 860
PspFI CCCAGC 2 cut(s) 218, 748
PspGI CCWGG 3 cut(s) 299, 338, 860
PspN4I GGNNCC 4 cut(s) 180, 181, 395, 859
PspOMI GGGCCC 1 cut(s) 178
PspPI GGNCC 6 cut(s) 44, 178, 179, 196, 216, 288
PstNI CAGNNNCTG 1 cut(s) 22
PsuI RGATCY 3 cut(s) 550, 826, 857
PsyI GACNNNGTC 1 cut(s) 70
PvuI CGATCG 1 cut(s) 476
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 2 cut(s) 296, 875
RsaNI GTAC 2 cut(s) 295, 874
SalI GTCGAC 1 cut(s) 64
SaqAI TTAA 1 cut(s) 831
Sau3AI GATC 8 cut(s) 237, 388, 435, 473, 550, 662, 826, 857
Sau96I GGNCC 6 cut(s) 44, 178, 179, 196, 216, 288
SchI GAGTC 6 cut(s) 266, 311, 477, 592, 719, 766
ScrFI CCNGG 6 cut(s) 176, 177, 301, 340, 521, 862
SduI GDGCHC 1 cut(s) 182
SfaNI GCATC 1 cut(s) 801
SfuI TTCGAA 2 cut(s) 488, 494
SgrAI CRCCGGYG 1 cut(s) 769
SmaI CCCGGG 1 cut(s) 177
Sse9I AATT 6 cut(s) 185, 478, 490, 616, 649, 715
SspMI CTAG 1 cut(s) 321
StyD4I CCNGG 6 cut(s) 174, 175, 299, 338, 519, 860
TaaI ACNGT 3 cut(s) 72, 262, 878
TaiI ACGT 1 cut(s) 65
TasI AATT 6 cut(s) 185, 478, 490, 616, 649, 715
TatI WGTACW 1 cut(s) 873
TauI GCSGC 4 cut(s) 22, 104, 257, 455
TfiI GAWTC 2 cut(s) 371, 414
Tru1I TTAA 1 cut(s) 831
Tru9I TTAA 1 cut(s) 831
TscAI CASTG 1 cut(s) 883
TseFI GTSAC 1 cut(s) 72
TseI GCWGC 7 cut(s) 5, 122, 125, 128, 131, 134, 208
Tsp45I GTSAC 1 cut(s) 72
TspGWI ACGGA 1 cut(s) 319
TspMI CCCGGG 1 cut(s) 175
TspRI CASTG 1 cut(s) 883
Tth111I GACNNNGTC 1 cut(s) 70
XapI RAATTY 1 cut(s) 490
XceI RCATGY 1 cut(s) 283
XmaI CCCGGG 1 cut(s) 175
XmiI GTMKAC 2 cut(s) 65, 764
XspI CTAG 1 cut(s) 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.