MD04G1000300.v1.1

Sterile alpha motif.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0003205
Physical Location & Seq
Forward (+)
29418 .. 29920
503 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1000300.v1.1.491

Sequence Viewer

Length: 309 bp
ATGCCGGAATCGAATTGGAGTGGAGTGAGGTCGTGGCTGATAGAATTGGGGCTGAGCCGGTACACGCCGGTGTTCGAGATACATGAGGTGGACGACGAGGTGCTGCCGATGCTGACATTGGAGGATCTTAAGGATATGGGAATCAACGCCGTTGGTTCGCGGCGGAAAATGTATGCCGCCATTCAGAAGCTCCGCAAGGGGTTCTCGCTGTGTACGATACACGTTCATTCCCCGACCCTCGCAAAGCGGAGACCCTTGTTGACTTGGTGTCACCCTTTTTTAATTGTAGTTTTCCCATCAATGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAM_1 SAM_2

Protein Analysis

103

Amino Acids

11.75

Weight (kDa)

8.71

Isoelectric Point (pI)

71.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAM_2 PF07647 8 - 65 3.2e-14 SAM domain (Sterile alpha motif)
SAM_1 PF00536 8 - 65 3.7e-14 SAM domain (Sterile alpha motif)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 160
AciI CCGC 5 cut(s) 160, 163, 177, 193, 247
AclWI GGATC 1 cut(s) 132
AfaI GTAC 2 cut(s) 62, 214
AflII CTTAAG 1 cut(s) 128
AflIII ACRYGT 1 cut(s) 220
AhdI GACNNNNNGTC 1 cut(s) 267
AleI CACNNNNGTG 1 cut(s) 68
AluBI AGCT 1 cut(s) 190
AluI AGCT 1 cut(s) 190
Alw26I GTCTC 1 cut(s) 244
AlwI GGATC 1 cut(s) 132
ApeKI GCWGC 1 cut(s) 103
AsuHPI GGTGA 1 cut(s) 263
BbvI GCAGC 1 cut(s) 90
BccI CCATC 1 cut(s) 304
BceAI ACGGC 1 cut(s) 134
BcoDI GTCTC 1 cut(s) 244
BfrI CTTAAG 1 cut(s) 128
BisI GCNGC 3 cut(s) 104, 161, 177
BlpI GCTNAGC 1 cut(s) 53
BlsI GCNGC 3 cut(s) 105, 162, 178
BmeRI GACNNNNNGTC 1 cut(s) 267
BmsI GCATC 1 cut(s) 99
Bpu1102I GCTNAGC 1 cut(s) 53
BsaI GGTCTC 1 cut(s) 244
Bse118I RCCGGY 2 cut(s) 57, 67
BseMII CTCAG 1 cut(s) 44
BseXI GCAGC 1 cut(s) 90
Bsh1236I CGCG 1 cut(s) 160
BsiSI CCGG 3 cut(s) 5, 58, 68
BsmAI GTCTC 1 cut(s) 244
Bso31I GGTCTC 1 cut(s) 244
Bsp143I GATC 1 cut(s) 124
Bsp1720I GCTNAGC 1 cut(s) 53
BspACI CCGC 5 cut(s) 160, 163, 177, 193, 247
BspCNI CTCAG 1 cut(s) 45
BspFNI CGCG 1 cut(s) 160
BspPI GGATC 1 cut(s) 132
BspTI CTTAAG 1 cut(s) 128
BspTNI GGTCTC 1 cut(s) 244
BsrFI RCCGGY 2 cut(s) 57, 67
BssAI RCCGGY 2 cut(s) 57, 67
BssMI GATC 1 cut(s) 124
BstAFI CTTAAG 1 cut(s) 128
BstDEI CTNAG 1 cut(s) 53
BstFNI CGCG 1 cut(s) 160
BstKTI GATC 1 cut(s) 127
BstMAI GTCTC 1 cut(s) 244
BstMBI GATC 1 cut(s) 124
BstMWI GCNNNNNNNGC 1 cut(s) 109
BstUI CGCG 1 cut(s) 160
BstV1I GCAGC 1 cut(s) 90
BstX2I RGATCY 1 cut(s) 124
BstYI RGATCY 1 cut(s) 124
Cfr10I RCCGGY 2 cut(s) 57, 67
Csp6I GTAC 2 cut(s) 61, 213
CviAII CATG 1 cut(s) 83
CviJI RGCY 4 cut(s) 37, 52, 57, 190
CviKI_1 RGCY 4 cut(s) 37, 52, 57, 190
CviQI GTAC 2 cut(s) 61, 213
DdeI CTNAG 1 cut(s) 53
DpnI GATC 1 cut(s) 126
DpnII GATC 1 cut(s) 124
DriI GACNNNNNGTC 1 cut(s) 267
Eam1105I GACNNNNNGTC 1 cut(s) 267
EciI GGCGGA 1 cut(s) 178
Eco31I GGTCTC 1 cut(s) 244
FaeI CATG 1 cut(s) 86
FaiI YATR 3 cut(s) 84, 137, 174
FatI CATG 1 cut(s) 82
Fnu4HI GCNGC 3 cut(s) 104, 161, 177
Fsp4HI GCNGC 3 cut(s) 104, 161, 177
GluI GCNGC 3 cut(s) 104, 161, 177
HapII CCGG 3 cut(s) 5, 58, 68
Hin1II CATG 1 cut(s) 86
HincII GTYRAC 1 cut(s) 261
HindII GTYRAC 1 cut(s) 261
HinfI GANTC 2 cut(s) 8, 141
HpaII CCGG 3 cut(s) 5, 58, 68
HphI GGTGA 1 cut(s) 263
Hpy166II GTNNAC 4 cut(s) 63, 91, 213, 261
Hpy188I TCNGA 1 cut(s) 186
Hpy188III TCNNGA 1 cut(s) 76
Hpy8I GTNNAC 4 cut(s) 63, 91, 213, 261
Hpy99I CGWCG 1 cut(s) 98
HpyCH4IV ACGT 1 cut(s) 222
HpyCH4V TGCA 1 cut(s) 304
HpyF10VI GCNNNNNNNGC 1 cut(s) 109
HpyF3I CTNAG 1 cut(s) 53
HpySE526I ACGT 1 cut(s) 222
Hsp92II CATG 1 cut(s) 86
Kzo9I GATC 1 cut(s) 124
LmnI GCTCC 1 cut(s) 195
LpnPI CCDG 3 cut(s) 18, 71, 81
Lsp1109I GCAGC 1 cut(s) 90
LweI GCATC 1 cut(s) 99
MaeII ACGT 1 cut(s) 222
MaeIII GTNAC 1 cut(s) 269
MalI GATC 1 cut(s) 126
MboI GATC 1 cut(s) 124
MflI RGATCY 1 cut(s) 124
MluCI AATT 3 cut(s) 13, 44, 282
MnlI CCTC 5 cut(s) 21, 79, 91, 115, 248
MseI TTAA 2 cut(s) 129, 281
MslI CAYNNNNRTG 1 cut(s) 68
MspCI CTTAAG 1 cut(s) 128
MspI CCGG 3 cut(s) 5, 58, 68
MvnI CGCG 1 cut(s) 160
MwoI GCNNNNNNNGC 1 cut(s) 109
NdeII GATC 1 cut(s) 124
NlaIII CATG 1 cut(s) 86
NmuCI GTSAC 1 cut(s) 269
OliI CACNNNNGTG 1 cut(s) 68
PcsI WCGNNNNNNNCGW 1 cut(s) 212
PfeI GAWTC 2 cut(s) 8, 141
PkrI GCNGC 3 cut(s) 105, 162, 178
PsuI RGATCY 1 cut(s) 124
RsaI GTAC 2 cut(s) 62, 214
RsaNI GTAC 2 cut(s) 61, 213
RseI CAYNNNNRTG 1 cut(s) 68
SaqAI TTAA 2 cut(s) 129, 281
SatI GCNGC 3 cut(s) 104, 161, 177
Sau3AI GATC 1 cut(s) 124
SetI ASST 5 cut(s) 32, 90, 102, 192, 225
SfaNI GCATC 1 cut(s) 99
SgrAI CRCCGGYG 1 cut(s) 67
SmiMI CAYNNNNRTG 1 cut(s) 68
SmlI CTYRAG 1 cut(s) 128
SmoI CTYRAG 1 cut(s) 128
Sse9I AATT 3 cut(s) 13, 44, 282
SsiI CCGC 5 cut(s) 160, 163, 177, 193, 247
TaiI ACGT 1 cut(s) 225
TaqI TCGA 2 cut(s) 11, 75
TasI AATT 3 cut(s) 13, 44, 282
TauI GCSGC 2 cut(s) 163, 179
TfiI GAWTC 2 cut(s) 8, 141
Tru1I TTAA 2 cut(s) 129, 281
Tru9I TTAA 2 cut(s) 129, 281
TseFI GTSAC 1 cut(s) 269
TseI GCWGC 1 cut(s) 103
Tsp45I GTSAC 1 cut(s) 269
TspDTI ATGAA 1 cut(s) 215
Vha464I CTTAAG 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.