Rh4CG311100

Sterile alpha motif.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0003205
Physical Location & Seq
Forward (+)
57259110 .. 57262311
3202 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG311100.1

Sequence Viewer

Length: 909 bp
ATGGCAGCTGAGTTACAGCCGCTGGAGGCTACAATCCCCGCCGGCCCAACCGCCACCGCCACGTCGACACCGTCACCTCTTATAACCCCAACGGCGACGGCCGCAATCGCAACCACAGCAGCAGCAGCAGCAGCAGCAGCAACAGCAACGGCTCTACACTCGGGCGAAAACCCGGGCCCCCAATTCCAACTGGGCCAGAAACGGCAGCGCCGGCCCAGCGTCCGGTTAGGCGAGATCGGCGACCAGCCCGCCGCCACCGTCTCAATCGACTCACACATGCGAAGGGCCAAGTACCCCTGGAAGCTCCCGAAGGACTCTAGTAAATCCGTCAAGGCCAGGTCGATTACGAACCTCGTGAACGGCCACGATTCGACTGAGATAGATGATCGGAACCAGAACGGCGACGGGGGATTCGAGTTCGGGAGCAGGAAGATCAAATCGAAGCGGGCGGCGACGACGAAGAGAGTCCGATCGAATTGGATTTCGAATTCGAAGATTGACGAGGGAGGGGATAGCCGGGAGGAGGGGGAGGGGTGGATGAGCGCAGATCCCGATAGGGATAGTCCGGTAAGGGATGAGAGCCCGGTGCAGTCGGCGGAGAACGAGGCGCACAATTGGGAGGAGCAGAGGAGGGTTGGGGGGAATAATTGGGGGAGGGGATCGGAGAGCAGGGAAAACGACGCCGTTGAGGCGGATAATATGCCGGAGTCGAATTGGAGCGGAGTGAGGTCGTGGCTAATAGAGCTGGGGTTGAGTCGGTATACGCCGGTGTTCGAGATACATGAGGTGGATGATGAGGTGTTGCCGATGCTGACATTGGAGGATCTTAAAGATATGGGGATAAACGCCGTTGGATCCAGGCGGAAAATGTACAGTGCTATCCAGAAGCTTCGCAAGGGCTTTTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAM_1 SAM_2

Protein Analysis

302

Amino Acids

32.88

Weight (kDa)

5.85

Isoelectric Point (pI)

57.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAM_1 PF00536 241 - 298 2.2e-13 SAM domain (Sterile alpha motif)
SAM_2 PF07647 242 - 298 1.3e-13 SAM domain (Sterile alpha motif)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 83
AccBSI CCGCTC 1 cut(s) 720
AccI GTMKAC 2 cut(s) 65, 761
AclWI GGATC 5 cut(s) 542, 667, 831, 849, 862
AcoI YGGCCR 2 cut(s) 99, 361
AcsI RAATTY 1 cut(s) 487
AcyI GRCGYC 1 cut(s) 681
AfaI GTAC 2 cut(s) 293, 872
AfiI CCNNNNNNNGG 2 cut(s) 222, 523
AjiI CACGTC 1 cut(s) 63
AjnI CCWGG 3 cut(s) 296, 335, 857
AluBI AGCT 4 cut(s) 8, 304, 745, 889
AluI AGCT 4 cut(s) 8, 304, 745, 889
Alw26I GTCTC 1 cut(s) 265
AlwI GGATC 5 cut(s) 542, 667, 831, 849, 862
AlwNI CAGNNNCTG 1 cut(s) 22
Ama87I CYCGRG 2 cut(s) 160, 172
AoxI GGCC 8 cut(s) 43, 99, 175, 193, 212, 285, 333, 361
ApaI GGGCCC 1 cut(s) 179
ApeKI GCWGC 9 cut(s) 5, 119, 122, 125, 128, 131, 134, 137, 205
ApoI RAATTY 1 cut(s) 487
ArsI GACNNNNNNTTYG 2 cut(s) 395, 427
AspLEI GCGC 3 cut(s) 210, 545, 610
AspS9I GGNCC 6 cut(s) 44, 175, 176, 193, 213, 285
AsuC2I CCSGG 4 cut(s) 173, 174, 518, 584
AsuHPI GGTGA 1 cut(s) 66
AsuII TTCGAA 2 cut(s) 485, 491
AvaI CYCGRG 2 cut(s) 160, 172
BaeGI GKGCMC 1 cut(s) 179
BamHI GGATCC 1 cut(s) 854
BanII GRGCYC 2 cut(s) 179, 584
BauI CACGAG 1 cut(s) 353
BbvI GCAGC 9 cut(s) 17, 131, 134, 137, 140, 143, 146, 149, 217
BceAI ACGGC 8 cut(s) 108, 114, 165, 218, 376, 415, 668, 833
BciT130I CCWGG 3 cut(s) 298, 337, 859
BcnI CCSGG 4 cut(s) 173, 174, 518, 584
BcoDI GTCTC 1 cut(s) 265
BfaI CTAG 1 cut(s) 318
BfoI RGCGCY 1 cut(s) 211
BglI GCCNNNNNGGC 1 cut(s) 689
Bme1390I CCNGG 7 cut(s) 173, 174, 298, 337, 518, 584, 859
BmeT110I CYCGRG 2 cut(s) 160, 172
BmgBI CACGTC 1 cut(s) 63
BmgT120I GGNCC 6 cut(s) 44, 175, 176, 193, 213, 285
BmiI GGNNCC 4 cut(s) 177, 178, 392, 856
BmrFI CCNGG 7 cut(s) 173, 174, 298, 337, 518, 584, 859
BmrI ACTGGG 1 cut(s) 200
BmsI GCATC 1 cut(s) 798
BmuI ACTGGG 1 cut(s) 200
BpmI CTGGAG 1 cut(s) 44
Bpu14I TTCGAA 2 cut(s) 485, 491
BpuMI CCSGG 4 cut(s) 173, 174, 518, 584
BsaHI GRCGYC 1 cut(s) 681
BsaJI CCNNGG 2 cut(s) 172, 296
BsaWI WCCGGW 2 cut(s) 222, 565
Bsc4I CCNNNNNNNGG 2 cut(s) 222, 523
Bse118I RCCGGY 3 cut(s) 41, 210, 766
Bse1I ACTGG 1 cut(s) 195
BseBI CCWGG 3 cut(s) 298, 337, 859
BseDI CCNNGG 2 cut(s) 172, 296
BseGI GGATG 3 cut(s) 543, 580, 796
BseLI CCNNNNNNNGG 2 cut(s) 222, 523
BseMII CTCAG 1 cut(s) 366
BseNI ACTGG 1 cut(s) 195
BseRI GAGGAG 3 cut(s) 536, 635, 643
BseSI GKGCMC 1 cut(s) 179
BseX3I CGGCCG 1 cut(s) 99
BseXI GCAGC 9 cut(s) 17, 131, 134, 137, 140, 143, 146, 149, 217
BseYI CCCAGC 2 cut(s) 215, 745
BsgI GTGCAG 1 cut(s) 608
Bsh1285I CGRYCG 2 cut(s) 102, 473
BshFI GGCC 8 cut(s) 45, 101, 177, 195, 214, 287, 335, 363
BsiEI CGRYCG 2 cut(s) 102, 473
BsiHKCI CYCGRG 2 cut(s) 160, 172
BsiSI CCGG 9 cut(s) 42, 173, 211, 223, 517, 566, 584, 704, 767
BslI CCNNNNNNNGG 2 cut(s) 222, 523
BsmAI GTCTC 1 cut(s) 265
BsmBI CGTCTC 1 cut(s) 265
BsnI GGCC 8 cut(s) 45, 101, 177, 195, 214, 287, 335, 363
BsoBI CYCGRG 2 cut(s) 160, 172
Bsp119I TTCGAA 2 cut(s) 485, 491
Bsp120I GGGCCC 1 cut(s) 175
Bsp1286I GDGCHC 2 cut(s) 179, 584
Bsp1407I TGTACA 1 cut(s) 870
Bsp143I GATC 8 cut(s) 234, 385, 432, 470, 547, 659, 823, 854
BspANI GGCC 8 cut(s) 45, 101, 177, 195, 214, 287, 335, 363
BspCNI CTCAG 1 cut(s) 367
BspLI GGNNCC 4 cut(s) 177, 178, 392, 856
BspPI GGATC 5 cut(s) 542, 667, 831, 849, 862
BspT104I TTCGAA 2 cut(s) 485, 491
BsrBI CCGCTC 1 cut(s) 720
BsrFI RCCGGY 3 cut(s) 41, 210, 766
BsrGI TGTACA 1 cut(s) 870
BsrI ACTGG 1 cut(s) 195
BssAI RCCGGY 3 cut(s) 41, 210, 766
BssECI CCNNGG 2 cut(s) 172, 296
BssMI GATC 8 cut(s) 234, 385, 432, 470, 547, 659, 823, 854
BssNAI GTATAC 1 cut(s) 762
BssNI GRCGYC 1 cut(s) 681
BssSI CACGAG 1 cut(s) 353
Bst1107I GTATAC 1 cut(s) 762
Bst2BI CACGAG 1 cut(s) 353
Bst2UI CCWGG 3 cut(s) 298, 337, 859
Bst4CI ACNGT 3 cut(s) 72, 259, 875
Bst6I CTCTTC 1 cut(s) 455
BstACI GRCGYC 1 cut(s) 681
BstAUI TGTACA 1 cut(s) 870
BstBI TTCGAA 2 cut(s) 485, 491
BstC8I GCNNGC 4 cut(s) 43, 212, 249, 447
BstDEI CTNAG 2 cut(s) 9, 375
BstF5I GGATG 3 cut(s) 543, 580, 796
BstH2I RGCGCY 1 cut(s) 211
BstHHI GCGC 3 cut(s) 210, 545, 610
BstKTI GATC 8 cut(s) 237, 388, 435, 473, 550, 662, 826, 857
BstMAI GTCTC 1 cut(s) 265
BstMBI GATC 8 cut(s) 234, 385, 432, 470, 547, 659, 823, 854
BstMCI CGRYCG 2 cut(s) 102, 473
BstNI CCWGG 3 cut(s) 298, 337, 859
BstNSI RCATGY 1 cut(s) 280
BstSCI CCNGG 7 cut(s) 171, 172, 296, 335, 516, 582, 857
BstSLI GKGCMC 1 cut(s) 179
BstV1I GCAGC 9 cut(s) 17, 131, 134, 137, 140, 143, 146, 149, 217
BstX2I RGATCY 3 cut(s) 547, 823, 854
BstYI RGATCY 3 cut(s) 547, 823, 854
BstZ17I GTATAC 1 cut(s) 762
BstZI CGGCCG 1 cut(s) 99
BsuRI GGCC 8 cut(s) 45, 101, 177, 195, 214, 287, 335, 363
BtrI CACGTC 1 cut(s) 63
BtsCI GGATG 3 cut(s) 543, 580, 796
BtsIMutI CAGTG 1 cut(s) 880
Cac8I GCNNGC 4 cut(s) 43, 212, 249, 447
CaiI CAGNNNCTG 1 cut(s) 22
CfoI GCGC 3 cut(s) 210, 545, 610
Cfr10I RCCGGY 3 cut(s) 41, 210, 766
Cfr13I GGNCC 6 cut(s) 44, 175, 176, 193, 213, 285
Cfr9I CCCGGG 1 cut(s) 172
CseI GACGC 2 cut(s) 208, 689
Csp6I GTAC 2 cut(s) 292, 871
CviAII CATG 2 cut(s) 277, 782
CviQI GTAC 2 cut(s) 292, 871
DdeI CTNAG 2 cut(s) 9, 375
DpnI GATC 8 cut(s) 236, 387, 434, 472, 549, 661, 825, 856
DpnII GATC 8 cut(s) 234, 385, 432, 470, 547, 659, 823, 854
EaeI YGGCCR 2 cut(s) 99, 361
EagI CGGCCG 1 cut(s) 99
Eam1104I CTCTTC 1 cut(s) 455
EarI CTCTTC 1 cut(s) 455
EciI GGCGGA 3 cut(s) 611, 707, 877
EclXI CGGCCG 1 cut(s) 99
Eco24I GRGCYC 2 cut(s) 179, 584
Eco52I CGGCCG 1 cut(s) 99
Eco88I CYCGRG 2 cut(s) 160, 172
EcoO109I RGGNCCY 1 cut(s) 176
EcoRI GAATTC 1 cut(s) 487
EcoRII CCWGG 3 cut(s) 296, 335, 857
EcoT38I GRGCYC 2 cut(s) 179, 584
Esp3I CGTCTC 1 cut(s) 265
FaeI CATG 2 cut(s) 280, 785
FaiI YATR 6 cut(s) 83, 278, 701, 762, 783, 836
FatI CATG 2 cut(s) 276, 781
FauI CCCGC 3 cut(s) 46, 256, 438
FblI GTMKAC 2 cut(s) 65, 761
FokI GGATG 3 cut(s) 550, 587, 803
FriOI GRGCYC 2 cut(s) 179, 584
FspBI CTAG 1 cut(s) 318
GlaI GCGC 3 cut(s) 209, 544, 609
GsaI CCCAGC 2 cut(s) 219, 749
GsuI CTGGAG 1 cut(s) 44
HaeII RGCGCY 1 cut(s) 211
HaeIII GGCC 8 cut(s) 45, 101, 177, 195, 214, 287, 335, 363
HapII CCGG 9 cut(s) 42, 173, 211, 223, 517, 566, 584, 704, 767
HgaI GACGC 2 cut(s) 208, 689
HhaI GCGC 3 cut(s) 210, 545, 610
Hin1I GRCGYC 1 cut(s) 681
Hin1II CATG 2 cut(s) 280, 785
Hin6I GCGC 3 cut(s) 208, 543, 608
HinP1I GCGC 3 cut(s) 208, 543, 608
HincII GTYRAC 1 cut(s) 66
HindII GTYRAC 1 cut(s) 66
HindIII AAGCTT 1 cut(s) 887
HinfI GANTC 7 cut(s) 269, 314, 368, 411, 465, 707, 754
HpaII CCGG 9 cut(s) 42, 173, 211, 223, 517, 566, 584, 704, 767
HphI GGTGA 1 cut(s) 66
Hpy166II GTNNAC 3 cut(s) 66, 358, 762
Hpy188I TCNGA 3 cut(s) 390, 470, 664
Hpy188III TCNNGA 7 cut(s) 307, 355, 421, 551, 775, 883, 906
Hpy8I GTNNAC 3 cut(s) 66, 358, 762
Hpy99I CGWCG 6 cut(s) 67, 100, 407, 457, 460, 683
HpyAV CCTTC 2 cut(s) 276, 304
HpyCH4III ACNGT 3 cut(s) 72, 259, 875
HpyCH4IV ACGT 1 cut(s) 62
HpyCH4V TGCA 1 cut(s) 589
HpyF3I CTNAG 2 cut(s) 9, 375
HpySE526I ACGT 1 cut(s) 62
Hsp92I GRCGYC 1 cut(s) 681
Hsp92II CATG 2 cut(s) 280, 785
HspAI GCGC 3 cut(s) 208, 543, 608
KroI GCCGGC 2 cut(s) 41, 210
KroNI GCCGGC 2 cut(s) 43, 212
Kzo9I GATC 8 cut(s) 234, 385, 432, 470, 547, 659, 823, 854
LmnI GCTCC 4 cut(s) 309, 423, 622, 717
Lsp1109I GCAGC 9 cut(s) 17, 131, 134, 137, 140, 143, 146, 149, 217
LweI GCATC 1 cut(s) 798
MaeI CTAG 1 cut(s) 318
MaeII ACGT 1 cut(s) 62
MaeIII GTNAC 2 cut(s) 12, 72
MalI GATC 8 cut(s) 236, 387, 434, 472, 549, 661, 825, 856
MbiI CCGCTC 1 cut(s) 720
MboI GATC 8 cut(s) 234, 385, 432, 470, 547, 659, 823, 854
MboII GAAGA 3 cut(s) 442, 472, 505
MfeI CAATTG 1 cut(s) 613
MflI RGATCY 3 cut(s) 547, 823, 854
MhlI GDGCHC 2 cut(s) 179, 584
MluCI AATT 6 cut(s) 182, 475, 487, 613, 646, 712
MlyI GAGTC 5 cut(s) 263, 308, 474, 716, 763
MmeI TCCRAC 2 cut(s) 211, 832
MroNI GCCGGC 2 cut(s) 41, 210
MseI TTAA 1 cut(s) 828
MspA1I CMGCKG 2 cut(s) 8, 22
MspI CCGG 9 cut(s) 42, 173, 211, 223, 517, 566, 584, 704, 767
MspR9I CCNGG 7 cut(s) 173, 174, 298, 337, 518, 584, 859
MunI CAATTG 1 cut(s) 613
MvaI CCWGG 3 cut(s) 298, 337, 859
NaeI GCCGGC 2 cut(s) 43, 212
NciI CCSGG 4 cut(s) 173, 174, 518, 584
NdeII GATC 8 cut(s) 234, 385, 432, 470, 547, 659, 823, 854
NgoMIV GCCGGC 2 cut(s) 41, 210
NlaIII CATG 2 cut(s) 280, 785
NlaIV GGNNCC 4 cut(s) 177, 178, 392, 856
NmuCI GTSAC 1 cut(s) 72
NspI RCATGY 1 cut(s) 280
NspV TTCGAA 2 cut(s) 485, 491
PcsI WCGNNNNNNNCGW 2 cut(s) 68, 411
PdiI GCCGGC 2 cut(s) 43, 212
PfeI GAWTC 2 cut(s) 368, 411
PflFI GACNNNGTC 1 cut(s) 70
Ple19I CGATCG 1 cut(s) 473
PleI GAGTC 5 cut(s) 263, 308, 473, 715, 762
PpsI GAGTC 5 cut(s) 263, 308, 473, 715, 762
PsiI TTATAA 1 cut(s) 83
Psp6I CCWGG 3 cut(s) 296, 335, 857
PspFI CCCAGC 2 cut(s) 215, 745
PspGI CCWGG 3 cut(s) 296, 335, 857
PspN4I GGNNCC 4 cut(s) 177, 178, 392, 856
PspOMI GGGCCC 1 cut(s) 175
PspPI GGNCC 6 cut(s) 44, 175, 176, 193, 213, 285
PstNI CAGNNNCTG 1 cut(s) 22
PsuI RGATCY 3 cut(s) 547, 823, 854
PsyI GACNNNGTC 1 cut(s) 70
PvuI CGATCG 1 cut(s) 473
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 2 cut(s) 293, 872
RsaNI GTAC 2 cut(s) 292, 871
SalI GTCGAC 1 cut(s) 64
SaqAI TTAA 1 cut(s) 828
Sau3AI GATC 8 cut(s) 234, 385, 432, 470, 547, 659, 823, 854
Sau96I GGNCC 6 cut(s) 44, 175, 176, 193, 213, 285
SchI GAGTC 5 cut(s) 263, 308, 474, 716, 763
ScrFI CCNGG 7 cut(s) 173, 174, 298, 337, 518, 584, 859
SduI GDGCHC 2 cut(s) 179, 584
SfaNI GCATC 1 cut(s) 798
SfuI TTCGAA 2 cut(s) 485, 491
SgrAI CRCCGGYG 1 cut(s) 766
SmaI CCCGGG 1 cut(s) 174
Sse9I AATT 6 cut(s) 182, 475, 487, 613, 646, 712
SspMI CTAG 1 cut(s) 318
StyD4I CCNGG 7 cut(s) 171, 172, 296, 335, 516, 582, 857
TaaI ACNGT 3 cut(s) 72, 259, 875
TaiI ACGT 1 cut(s) 65
TasI AATT 6 cut(s) 182, 475, 487, 613, 646, 712
TatI WGTACW 1 cut(s) 870
TauI GCSGC 4 cut(s) 22, 104, 254, 452
TfiI GAWTC 2 cut(s) 368, 411
Tru1I TTAA 1 cut(s) 828
Tru9I TTAA 1 cut(s) 828
TscAI CASTG 1 cut(s) 880
TseFI GTSAC 1 cut(s) 72
TseI GCWGC 9 cut(s) 5, 119, 122, 125, 128, 131, 134, 137, 205
Tsp45I GTSAC 1 cut(s) 72
TspGWI ACGGA 1 cut(s) 316
TspMI CCCGGG 1 cut(s) 172
TspRI CASTG 1 cut(s) 880
Tth111I GACNNNGTC 1 cut(s) 70
XapI RAATTY 1 cut(s) 487
XceI RCATGY 1 cut(s) 280
XmaI CCCGGG 1 cut(s) 172
XmiI GTMKAC 2 cut(s) 65, 761
XspI CTAG 1 cut(s) 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.