FvH4_6g17310

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001935
Physical Location & Seq
Forward (+)
11229650 .. 11231536
1887 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g17310.t1

Sequence Viewer

Length: 1155 bp
ATGAAGTTCTTATATCATTCCATTCTTTTCTTCATTCATCTCTCTGCTCTATTCATGAACGCAAATGCTAGCCCTAGTTATATTATTGGCTCCTCTCGCCTCACGAAAGTTTCATCGCCTCTGTTTAAAGACTCGTGGGCAACCATGGAACTTGACGACGTAAAGATTGCGACGACACCAAACGTCGTTGTTTCACAAGATGGGAAGGGAAATTTCAAGAAAATAATGGATGCGATTGCAGCTGCACCAAGTCAGTTGTCATCCAAGCACTTTGTGATACTTGTTAAGAAAGGGGTTTACAAGGAATATGTGAACATCGGCAACACAAAGTCGAATATAGTCTTGATCGGTGAGGGTAAGGATGCTACTATCATTTCCGGAAGCAAAAGCGTAGGCGGCGGTCAGGCAACATTTGATACAGCTACATTTGCCGTTAGGGCTCAAGGATTTGTTGCGATGGACATAGGATTCGAGAACACAGCAGATCCAAAAAGTGGGCAAGCCGTGGCAGTTCAATCCAGCGGCGACCACTCTGTCTTCTACCGGTGCAAGATATCAGGAAACCAAGACACATTGCTTGTGCAAGCCGGCCGCCAGTTCTTCCGAGAATGCCAAATCATCGGCAGTATCGACTTCATCTTCGGCTATGGCACTGCCGTCTTCCAAAAATGTGACATTTACATCAAGAAAAGCCAAATCGGCGGCACCACCGTCATCACCGCCAACGGCCGTAACTCCTCAACAGACTCATCAGGCTTTTCGTTCCAATTTTGCACCATCCATGGTGACCCCCAGTCCCCTACTGGCCCTACATTCGTAACTCCTAGAGGTGTGTACCTCGGTCGAAGCTGGGGCACTTATGCCAGAACAGTGTTCATGCAGTCGTCTATAAGCAGTATTTTAAGCCCGCAAGGTTGGCTTCAATGGAATGATGTTCCAGTAGACAAGCTATACTTCGGTGAGTATCAAAATTCCGGCCCCGGTGCGGCGCTTGGAGGTCGGGTGACATGGCCGGGGTTTCATAAGATGAGCGCAGCCGATGCTGATAAATTTACAGTGGTCAAGTTTATTGATGGAGATTCATGGCTACCGGCGTTAGGTATCCCACACTCACAAGGATTGGATCCATTGGCCAATCCGCCAATGGGAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
PMEI Pectinesterase

Protein Analysis

385

Amino Acids

41.32

Weight (kDa)

8.87

Isoelectric Point (pI)

35.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 61 - 360 3.7e-110 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 533
AccB1I GGYRCC 1 cut(s) 704
AccB7I CCANNNNNTGG 1 cut(s) 494
AccI GTMKAC 1 cut(s) 942
AccIII TCCGGA 1 cut(s) 377
AciI CCGC 9 cut(s) 396, 399, 522, 592, 702, 720, 908, 986, 1139
AclWI GGATC 3 cut(s) 479, 1118, 1131
AcoI YGGCCR 4 cut(s) 589, 727, 1010, 1131
AcsI RAATTY 3 cut(s) 211, 970, 1049
AdeI CACNNNGTG 1 cut(s) 274
AfaI GTAC 1 cut(s) 836
AfiI CCNNNNNNNGG 4 cut(s) 494, 985, 1097, 1145
AgeI ACCGGT 1 cut(s) 543
AgsI TTSAA 3 cut(s) 217, 515, 923
AhdI GACNNNNNGTC 1 cut(s) 793
AluBI AGCT 5 cut(s) 242, 422, 849, 949, 1151
AluI AGCT 5 cut(s) 242, 422, 849, 949, 1151
AlwI GGATC 3 cut(s) 479, 1118, 1131
Aor13HI TCCGGA 1 cut(s) 377
AoxI GGCC 6 cut(s) 589, 727, 805, 976, 1010, 1131
ApeKI GCWGC 3 cut(s) 239, 242, 1034
ApoI RAATTY 3 cut(s) 211, 970, 1049
ArsI GACNNNNNNTTYG 4 cut(s) 452, 484, 623, 655
AsiGI ACCGGT 1 cut(s) 543
AspLEI GCGC 2 cut(s) 991, 1034
AspS9I GGNCC 2 cut(s) 806, 977
AsuC2I CCSGG 2 cut(s) 981, 1014
AsuHPI GGTGA 5 cut(s) 362, 709, 797, 971, 1015
AsuNHI GCTAGC 1 cut(s) 68
BaeGI GKGCMC 1 cut(s) 857
BalI TGGCCA 1 cut(s) 1133
BamHI GGATCC 1 cut(s) 1123
BanI GGYRCC 1 cut(s) 704
BanII GRGCYC 1 cut(s) 442
BauI CACGAG 1 cut(s) 133
BbsI GAAGAC 2 cut(s) 529, 652
BbvI GCAGC 3 cut(s) 229, 251, 1046
BccI CCATC 4 cut(s) 194, 451, 785, 1067
BceAI ACGGC 5 cut(s) 416, 488, 641, 714, 742
BciVI GTATCC 1 cut(s) 1112
BcnI CCSGG 2 cut(s) 981, 1014
BfaI CTAG 3 cut(s) 69, 75, 825
BfoI RGCGCY 1 cut(s) 992
BfuI GTATCC 1 cut(s) 1112
BglI GCCNNNNNGGC 2 cut(s) 437, 699
BisI GCNGC 8 cut(s) 240, 243, 397, 523, 592, 703, 987, 1035
BlsI GCNGC 8 cut(s) 241, 244, 398, 524, 593, 704, 988, 1036
Bme1390I CCNGG 2 cut(s) 981, 1014
BmeRI GACNNNNNGTC 1 cut(s) 793
BmgT120I GGNCC 2 cut(s) 806, 977
BmiI GGNNCC 4 cut(s) 91, 706, 979, 1125
BmrFI CCNGG 2 cut(s) 981, 1014
BmrI ACTGGG 1 cut(s) 787
BmsI GCATC 3 cut(s) 220, 352, 1030
BmtI GCTAGC 1 cut(s) 72
BmuI ACTGGG 1 cut(s) 787
BpiI GAAGAC 2 cut(s) 529, 652
BpuEI CTTGAG 1 cut(s) 426
BpuMI CCSGG 2 cut(s) 981, 1014
BsaJI CCNNGG 6 cut(s) 144, 504, 781, 838, 979, 1013
BsaWI WCCGGW 2 cut(s) 377, 543
BsaXI ACNNNNNCTCC 2 cut(s) 987, 1017
Bsc4I CCNNNNNNNGG 4 cut(s) 494, 985, 1097, 1145
Bse118I RCCGGY 3 cut(s) 543, 587, 1090
Bse1I ACTGG 4 cut(s) 595, 793, 808, 938
Bse3DI GCAATG 1 cut(s) 572
BseAI TCCGGA 1 cut(s) 377
BseDI CCNNGG 6 cut(s) 144, 504, 781, 838, 979, 1013
BseGI GGATG 4 cut(s) 235, 260, 367, 777
BseLI CCNNNNNNNGG 4 cut(s) 494, 985, 1097, 1145
BseMI GCAATG 1 cut(s) 572
BseNI ACTGG 4 cut(s) 595, 793, 808, 938
BseRI GAGGAG 2 cut(s) 82, 727
BseSI GKGCMC 1 cut(s) 857
BseX3I CGGCCG 2 cut(s) 589, 727
BseXI GCAGC 3 cut(s) 229, 251, 1046
BseYI CCCAGC 1 cut(s) 849
BsgI GTGCAG 1 cut(s) 228
Bsh1285I CGRYCG 3 cut(s) 592, 730, 844
BshFI GGCC 6 cut(s) 591, 729, 807, 978, 1012, 1133
BshNI GGYRCC 1 cut(s) 704
BshTI ACCGGT 1 cut(s) 543
BsiEI CGRYCG 3 cut(s) 592, 730, 844
BsiSI CCGG 7 cut(s) 378, 544, 588, 975, 981, 1013, 1091
BslFI GGGAC 1 cut(s) 781
BslI CCNNNNNNNGG 4 cut(s) 494, 985, 1097, 1145
BsmFI GGGAC 1 cut(s) 781
BsmI GAATGC 1 cut(s) 614
BsnI GGCC 6 cut(s) 591, 729, 807, 978, 1012, 1133
Bsp1286I GDGCHC 2 cut(s) 442, 857
Bsp13I TCCGGA 1 cut(s) 377
Bsp143I GATC 3 cut(s) 345, 484, 1123
Bsp19I CCATGG 2 cut(s) 144, 781
BspACI CCGC 9 cut(s) 396, 399, 522, 592, 702, 720, 908, 986, 1139
BspANI GGCC 6 cut(s) 591, 729, 807, 978, 1012, 1133
BspEI TCCGGA 1 cut(s) 377
BspHI TCATGA 1 cut(s) 54
BspLI GGNNCC 4 cut(s) 91, 706, 979, 1125
BspOI GCTAGC 1 cut(s) 72
BspPI GGATC 3 cut(s) 479, 1118, 1131
BspT107I GGYRCC 1 cut(s) 704
BsrDI GCAATG 1 cut(s) 572
BsrFI RCCGGY 3 cut(s) 543, 587, 1090
BsrI ACTGG 4 cut(s) 595, 793, 808, 938
BssAI RCCGGY 3 cut(s) 543, 587, 1090
BssECI CCNNGG 6 cut(s) 144, 504, 781, 838, 979, 1013
BssMI GATC 3 cut(s) 345, 484, 1123
BssSI CACGAG 1 cut(s) 133
BssT1I CCWWGG 2 cut(s) 144, 781
Bst2BI CACGAG 1 cut(s) 133
Bst4CI ACNGT 3 cut(s) 712, 871, 1057
BstAPI GCANNNNNTGC 1 cut(s) 1040
BstC8I GCNNGC 5 cut(s) 70, 501, 585, 589, 908
BstDSI CCRYGG 3 cut(s) 144, 504, 781
BstEII GGTNACC 1 cut(s) 785
BstF5I GGATG 4 cut(s) 235, 260, 367, 777
BstH2I RGCGCY 1 cut(s) 992
BstHHI GCGC 2 cut(s) 991, 1034
BstKTI GATC 3 cut(s) 348, 487, 1126
BstMBI GATC 3 cut(s) 345, 484, 1123
BstMCI CGRYCG 3 cut(s) 592, 730, 844
BstMWI GCNNNNNNNGC 8 cut(s) 96, 239, 396, 428, 437, 699, 916, 1040
BstPI GGTNACC 1 cut(s) 785
BstSCI CCNGG 2 cut(s) 979, 1012
BstSLI GKGCMC 1 cut(s) 857
BstV1I GCAGC 3 cut(s) 229, 251, 1046
BstV2I GAAGAC 2 cut(s) 529, 652
BstX2I RGATCY 2 cut(s) 484, 1123
BstYI RGATCY 2 cut(s) 484, 1123
BstZI CGGCCG 2 cut(s) 589, 727
BsuI GTATCC 1 cut(s) 1112
BsuRI GGCC 6 cut(s) 591, 729, 807, 978, 1012, 1133
BtgI CCRYGG 3 cut(s) 144, 504, 781
BtgZI GCGATG 2 cut(s) 99, 470
BtsCI GGATG 4 cut(s) 235, 260, 367, 777
BtsI GCAGTG 1 cut(s) 651
BtsIMutI CAGTG 3 cut(s) 651, 876, 1062
Cac8I GCNNGC 5 cut(s) 70, 501, 585, 589, 908
CciI TCATGA 1 cut(s) 54
CfoI GCGC 2 cut(s) 991, 1034
Cfr10I RCCGGY 3 cut(s) 543, 587, 1090
Cfr13I GGNCC 2 cut(s) 806, 977
Csp6I GTAC 1 cut(s) 835
CspAI ACCGGT 1 cut(s) 543
CviAII CATG 6 cut(s) 55, 145, 782, 877, 1008, 1083
CviQI GTAC 1 cut(s) 835
DpnI GATC 3 cut(s) 347, 486, 1125
DpnII GATC 3 cut(s) 345, 484, 1123
DraI TTTAAA 1 cut(s) 127
DraIII CACNNNGTG 1 cut(s) 274
DrdI GACNNNNNNGTC 1 cut(s) 533
DriI GACNNNNNGTC 1 cut(s) 793
DseDI GACNNNNNNGTC 1 cut(s) 533
EaeI YGGCCR 4 cut(s) 589, 727, 1010, 1131
EagI CGGCCG 2 cut(s) 589, 727
Eam1105I GACNNNNNGTC 1 cut(s) 793
EciI GGCGGA 1 cut(s) 1128
EclXI CGGCCG 2 cut(s) 589, 727
Eco130I CCWWGG 2 cut(s) 144, 781
Eco24I GRGCYC 1 cut(s) 442
Eco32I GATATC 1 cut(s) 555
Eco52I CGGCCG 2 cut(s) 589, 727
Eco91I GGTNACC 1 cut(s) 785
EcoO65I GGTNACC 1 cut(s) 785
EcoRV GATATC 1 cut(s) 555
EcoT14I CCWWGG 2 cut(s) 144, 781
EcoT38I GRGCYC 1 cut(s) 442
ErhI CCWWGG 2 cut(s) 144, 781
FaeI CATG 6 cut(s) 58, 148, 785, 880, 1011, 1086
FalI AAGNNNNNCTT 4 cut(s) 903, 935, 938, 970
FaqI GGGAC 1 cut(s) 781
FatI CATG 6 cut(s) 54, 144, 781, 876, 1007, 1082
FauI CCCGC 1 cut(s) 915
FblI GTMKAC 1 cut(s) 942
Fnu4HI GCNGC 8 cut(s) 240, 243, 397, 523, 592, 703, 987, 1035
FokI GGATG 4 cut(s) 242, 247, 374, 764
FriOI GRGCYC 1 cut(s) 442
Fsp4HI GCNGC 8 cut(s) 240, 243, 397, 523, 592, 703, 987, 1035
FspBI CTAG 3 cut(s) 69, 75, 825
GlaI GCGC 2 cut(s) 990, 1033
GluI GCNGC 8 cut(s) 240, 243, 397, 523, 592, 703, 987, 1035
GsaI CCCAGC 1 cut(s) 853
HaeII RGCGCY 1 cut(s) 992
HaeIII GGCC 6 cut(s) 591, 729, 807, 978, 1012, 1133
HapII CCGG 7 cut(s) 378, 544, 588, 975, 981, 1013, 1091
HhaI GCGC 2 cut(s) 991, 1034
Hin1II CATG 6 cut(s) 58, 148, 785, 880, 1011, 1086
Hin6I GCGC 2 cut(s) 989, 1032
HinP1I GCGC 2 cut(s) 989, 1032
HinfI GANTC 4 cut(s) 131, 468, 746, 1079
HpaII CCGG 7 cut(s) 378, 544, 588, 975, 981, 1013, 1091
HphI GGTGA 5 cut(s) 362, 709, 797, 971, 1015
Hpy166II GTNNAC 4 cut(s) 298, 313, 835, 943
Hpy188I TCNGA 1 cut(s) 605
Hpy188III TCNNGA 8 cut(s) 55, 103, 217, 343, 378, 472, 558, 685
Hpy8I GTNNAC 4 cut(s) 298, 313, 835, 943
Hpy99I CGWCG 3 cut(s) 161, 175, 188
HpyAV CCTTC 1 cut(s) 199
HpyCH4III ACNGT 3 cut(s) 712, 871, 1057
HpyCH4IV ACGT 2 cut(s) 159, 183
HpyCH4V TGCA 6 cut(s) 239, 245, 549, 583, 774, 880
HpyF10VI GCNNNNNNNGC 8 cut(s) 96, 239, 396, 428, 437, 699, 916, 1040
HpySE526I ACGT 2 cut(s) 159, 183
Hsp92II CATG 6 cut(s) 58, 148, 785, 880, 1011, 1086
HspAI GCGC 2 cut(s) 989, 1032
Kpn2I TCCGGA 1 cut(s) 377
KroI GCCGGC 1 cut(s) 587
KroNI GCCGGC 1 cut(s) 589
Kzo9I GATC 3 cut(s) 345, 484, 1123
LmnI GCTCC 2 cut(s) 95, 1148
Lsp1109I GCAGC 3 cut(s) 229, 251, 1046
LweI GCATC 3 cut(s) 220, 352, 1030
MaeI CTAG 3 cut(s) 69, 75, 825
MaeII ACGT 2 cut(s) 159, 183
MaeIII GTNAC 5 cut(s) 671, 731, 785, 817, 1003
MalI GATC 3 cut(s) 347, 486, 1125
MboI GATC 3 cut(s) 345, 484, 1123
MboII GAAGA 5 cut(s) 22, 529, 592, 631, 652
MflI RGATCY 2 cut(s) 484, 1123
MhlI GDGCHC 2 cut(s) 442, 857
MlsI TGGCCA 1 cut(s) 1133
MluCI AATT 4 cut(s) 211, 767, 970, 1049
MluNI TGGCCA 1 cut(s) 1133
MlyI GAGTC 2 cut(s) 125, 740
MnlI CCTC 8 cut(s) 103, 110, 129, 346, 748, 821, 848, 989
Mox20I TGGCCA 1 cut(s) 1133
MroI TCCGGA 1 cut(s) 377
MroNI GCCGGC 1 cut(s) 587
MscI TGGCCA 1 cut(s) 1133
MseI TTAA 4 cut(s) 126, 285, 902, 1153
Msp20I TGGCCA 1 cut(s) 1133
MspA1I CMGCKG 2 cut(s) 242, 522
MspI CCGG 7 cut(s) 378, 544, 588, 975, 981, 1013, 1091
MspR9I CCNGG 2 cut(s) 981, 1014
Mva1269I GAATGC 1 cut(s) 614
MwoI GCNNNNNNNGC 8 cut(s) 96, 239, 396, 428, 437, 699, 916, 1040
NaeI GCCGGC 1 cut(s) 589
NciI CCSGG 2 cut(s) 981, 1014
NcoI CCATGG 2 cut(s) 144, 781
NdeII GATC 3 cut(s) 345, 484, 1123
NgoMIV GCCGGC 1 cut(s) 587
NheI GCTAGC 1 cut(s) 68
NlaIII CATG 6 cut(s) 58, 148, 785, 880, 1011, 1086
NlaIV GGNNCC 4 cut(s) 91, 706, 979, 1125
NmuCI GTSAC 3 cut(s) 671, 785, 1003
PagI TCATGA 1 cut(s) 54
PcsI WCGNNNNNNNCGW 1 cut(s) 627
PctI GAATGC 1 cut(s) 614
PdiI GCCGGC 1 cut(s) 589
PfeI GAWTC 2 cut(s) 468, 1079
PflMI CCANNNNNTGG 1 cut(s) 494
PinAI ACCGGT 1 cut(s) 543
PkrI GCNGC 8 cut(s) 241, 244, 398, 524, 593, 704, 988, 1036
PleI GAGTC 2 cut(s) 125, 740
PpsI GAGTC 2 cut(s) 125, 740
PspEI GGTNACC 1 cut(s) 785
PspFI CCCAGC 1 cut(s) 849
PspN4I GGNNCC 4 cut(s) 91, 706, 979, 1125
PspPI GGNCC 2 cut(s) 806, 977
PsuI RGATCY 2 cut(s) 484, 1123
PvuII CAGCTG 1 cut(s) 242
RsaI GTAC 1 cut(s) 836
RsaNI GTAC 1 cut(s) 835
SaqAI TTAA 4 cut(s) 126, 285, 902, 1153
SatI GCNGC 8 cut(s) 240, 243, 397, 523, 592, 703, 987, 1035
Sau3AI GATC 3 cut(s) 345, 484, 1123
Sau96I GGNCC 2 cut(s) 806, 977
SchI GAGTC 2 cut(s) 125, 740
ScrFI CCNGG 2 cut(s) 981, 1014
SduI GDGCHC 2 cut(s) 442, 857
SfaNI GCATC 3 cut(s) 220, 352, 1030
SmlI CTYRAG 1 cut(s) 441
SmoI CTYRAG 1 cut(s) 441
Sse9I AATT 4 cut(s) 211, 767, 970, 1049
SsiI CCGC 9 cut(s) 396, 399, 522, 592, 702, 720, 908, 986, 1139
SspMI CTAG 3 cut(s) 69, 75, 825
StyD4I CCNGG 2 cut(s) 979, 1012
StyI CCWWGG 2 cut(s) 144, 781
TaaI ACNGT 3 cut(s) 712, 871, 1057
TaiI ACGT 2 cut(s) 162, 186
TaqI TCGA 4 cut(s) 332, 471, 630, 844
TaqII GACCGA 1 cut(s) 830
TasI AATT 4 cut(s) 211, 767, 970, 1049
TauI GCSGC 5 cut(s) 399, 525, 594, 705, 989
TfiI GAWTC 2 cut(s) 468, 1079
Tru1I TTAA 4 cut(s) 126, 285, 902, 1153
Tru9I TTAA 4 cut(s) 126, 285, 902, 1153
TscAI CASTG 3 cut(s) 658, 876, 1062
TseFI GTSAC 3 cut(s) 671, 785, 1003
TseI GCWGC 3 cut(s) 239, 242, 1034
Tsp45I GTSAC 3 cut(s) 671, 785, 1003
TspRI CASTG 3 cut(s) 658, 876, 1062
Van91I CCANNNNNTGG 1 cut(s) 494
XapI RAATTY 3 cut(s) 211, 970, 1049
XcmI CCANNNNNNNNNTGG 2 cut(s) 800, 1141
XmiI GTMKAC 1 cut(s) 942
XspI CTAG 3 cut(s) 69, 75, 825
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.