RchiOBHm_Chr1g0362911

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001935
Physical Location & Seq
Forward (+)
54484660 .. 54484911
252 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ58769

Sequence Viewer

Length: 192 bp
ATGGAAACCGTGGCATTGACACTGTATTATGGTGAGTTCATGAATAAAGGGCCAGGTTCCGAGCTCGCAGGGCGGGTCAGATGGCTCGGTCATCATACTGATCCATCTGAGGCCGATCAGTTTACGGCCACCAACTTCATTGACGGAGATTCTTGGCTGCCATCCACAGGTATCCCATACACATCAACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
PMEI Pectinesterase

Protein Analysis

63

Amino Acids

6.94

Weight (kDa)

4.49

Isoelectric Point (pI)

30.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 6 - 50 1.1e-12 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 73
AclWI GGATC 1 cut(s) 95
AcoI YGGCCR 1 cut(s) 126
AfiI CCNNNNNNNGG 1 cut(s) 167
AjnI CCWGG 1 cut(s) 52
AluBI AGCT 1 cut(s) 64
AluI AGCT 1 cut(s) 64
Alw21I GWGCWC 1 cut(s) 66
AlwI GGATC 1 cut(s) 95
AoxI GGCC 3 cut(s) 50, 111, 126
ApeKI GCWGC 1 cut(s) 157
AspS9I GGNCC 1 cut(s) 50
AsuHPI GGTGA 1 cut(s) 44
BanII GRGCYC 1 cut(s) 66
Bbv12I GWGCWC 1 cut(s) 66
BbvI GCAGC 1 cut(s) 144
BccI CCATC 3 cut(s) 75, 112, 169
BceAI ACGGC 1 cut(s) 141
BciT130I CCWGG 1 cut(s) 54
BciVI GTATCC 1 cut(s) 182
BfaI CTAG 1 cut(s) 190
BfuI GTATCC 1 cut(s) 182
BisI GCNGC 1 cut(s) 158
BlsI GCNGC 1 cut(s) 159
Bme1390I CCNGG 1 cut(s) 54
BmgT120I GGNCC 1 cut(s) 50
BmiI GGNNCC 1 cut(s) 58
BmrFI CCNGG 1 cut(s) 54
BsaJI CCNNGG 1 cut(s) 9
Bsc4I CCNNNNNNNGG 1 cut(s) 167
BseBI CCWGG 1 cut(s) 54
BseDI CCNNGG 1 cut(s) 9
BseGI GGATG 1 cut(s) 161
BseLI CCNNNNNNNGG 1 cut(s) 167
BseMII CTCAG 1 cut(s) 99
BseXI GCAGC 1 cut(s) 144
BshFI GGCC 3 cut(s) 52, 113, 128
BsiHKAI GWGCWC 1 cut(s) 66
BslI CCNNNNNNNGG 1 cut(s) 167
BsnI GGCC 3 cut(s) 52, 113, 128
Bsp1286I GDGCHC 1 cut(s) 66
Bsp143I GATC 2 cut(s) 100, 115
BspACI CCGC 1 cut(s) 73
BspANI GGCC 3 cut(s) 52, 113, 128
BspCNI CTCAG 1 cut(s) 100
BspHI TCATGA 1 cut(s) 39
BspLI GGNNCC 1 cut(s) 58
BspPI GGATC 1 cut(s) 95
BssECI CCNNGG 1 cut(s) 9
BssMI GATC 2 cut(s) 100, 115
Bst2UI CCWGG 1 cut(s) 54
Bst4CI ACNGT 2 cut(s) 10, 24
BstC8I GCNNGC 1 cut(s) 66
BstDEI CTNAG 1 cut(s) 108
BstDSI CCRYGG 1 cut(s) 9
BstF5I GGATG 1 cut(s) 161
BstKTI GATC 2 cut(s) 103, 118
BstMBI GATC 2 cut(s) 100, 115
BstMWI GCNNNNNNNGC 1 cut(s) 70
BstNI CCWGG 1 cut(s) 54
BstSCI CCNGG 1 cut(s) 52
BstV1I GCAGC 1 cut(s) 144
BsuI GTATCC 1 cut(s) 182
BsuRI GGCC 3 cut(s) 52, 113, 128
BtgI CCRYGG 1 cut(s) 9
BtsCI GGATG 1 cut(s) 161
BtsIMutI CAGTG 1 cut(s) 20
Cac8I GCNNGC 1 cut(s) 66
CciI TCATGA 1 cut(s) 39
Cfr13I GGNCC 1 cut(s) 50
CviAII CATG 1 cut(s) 40
CviJI RGCY 6 cut(s) 52, 64, 85, 113, 128, 157
CviKI_1 RGCY 6 cut(s) 52, 64, 85, 113, 128, 157
DdeI CTNAG 1 cut(s) 108
DpnI GATC 2 cut(s) 102, 117
DpnII GATC 2 cut(s) 100, 115
EaeI YGGCCR 1 cut(s) 126
Ecl136II GAGCTC 1 cut(s) 64
Eco24I GRGCYC 1 cut(s) 66
Eco53kI GAGCTC 1 cut(s) 64
EcoICRI GAGCTC 1 cut(s) 64
EcoRII CCWGG 1 cut(s) 52
EcoT38I GRGCYC 1 cut(s) 66
FaeI CATG 1 cut(s) 43
FaiI YATR 4 cut(s) 30, 41, 96, 178
FatI CATG 1 cut(s) 39
FauI CCCGC 1 cut(s) 66
Fnu4HI GCNGC 1 cut(s) 158
FokI GGATG 1 cut(s) 148
FriOI GRGCYC 1 cut(s) 66
Fsp4HI GCNGC 1 cut(s) 158
FspBI CTAG 1 cut(s) 190
GluI GCNGC 1 cut(s) 158
HaeIII GGCC 3 cut(s) 52, 113, 128
Hin1II CATG 1 cut(s) 43
HinfI GANTC 1 cut(s) 149
HphI GGTGA 1 cut(s) 44
Hpy166II GTNNAC 1 cut(s) 123
Hpy188I TCNGA 3 cut(s) 61, 80, 109
Hpy188III TCNNGA 1 cut(s) 40
Hpy8I GTNNAC 1 cut(s) 123
HpyCH4III ACNGT 2 cut(s) 10, 24
HpyF10VI GCNNNNNNNGC 1 cut(s) 70
HpyF3I CTNAG 1 cut(s) 108
Hsp92II CATG 1 cut(s) 43
Kzo9I GATC 2 cut(s) 100, 115
LpnPI CCDG 4 cut(s) 39, 54, 66, 153
Lsp1109I GCAGC 1 cut(s) 144
MaeI CTAG 1 cut(s) 190
MalI GATC 2 cut(s) 102, 117
MboI GATC 2 cut(s) 100, 115
MhlI GDGCHC 1 cut(s) 66
MnlI CCTC 1 cut(s) 103
MspR9I CCNGG 1 cut(s) 54
MvaI CCWGG 1 cut(s) 54
MwoI GCNNNNNNNGC 1 cut(s) 70
NdeII GATC 2 cut(s) 100, 115
NlaIII CATG 1 cut(s) 43
NlaIV GGNNCC 1 cut(s) 58
PagI TCATGA 1 cut(s) 39
PfeI GAWTC 1 cut(s) 149
PkrI GCNGC 1 cut(s) 159
Psp124BI GAGCTC 1 cut(s) 66
Psp6I CCWGG 1 cut(s) 52
PspGI CCWGG 1 cut(s) 52
PspN4I GGNNCC 1 cut(s) 58
PspPI GGNCC 1 cut(s) 50
SacI GAGCTC 1 cut(s) 66
SatI GCNGC 1 cut(s) 158
Sau3AI GATC 2 cut(s) 100, 115
Sau96I GGNCC 1 cut(s) 50
ScrFI CCNGG 1 cut(s) 54
SduI GDGCHC 1 cut(s) 66
SetI ASST 4 cut(s) 58, 66, 172, 191
SsiI CCGC 1 cut(s) 73
SspMI CTAG 1 cut(s) 190
SstI GAGCTC 1 cut(s) 66
StyD4I CCNGG 1 cut(s) 52
TaaI ACNGT 2 cut(s) 10, 24
TaqII GACCGA 1 cut(s) 77
TfiI GAWTC 1 cut(s) 149
TscAI CASTG 1 cut(s) 27
TseI GCWGC 1 cut(s) 157
TspDTI ATGAA 3 cut(s) 28, 56, 127
TspGWI ACGGA 1 cut(s) 159
TspRI CASTG 1 cut(s) 27
XspI CTAG 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.