Prupe.2G216300_v2.0.a1

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0001935
Physical Location & Seq
Forward (+)
24667563 .. 24670565
3003 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G216300.1

Sequence Viewer

Length: 900 bp
ATGACCAGAACATGGCTGAGTACTTCGCAATCCAAAGAAAAGAAATGCGTGGACACTTTTCAAAATATGATCGACTCAAATGTTGCGGAGAGCCTTAGACAAGTGACAAAATCAATCGATGAGGTCCTTGGTATGATCCAAGTGCAGCAGCAACATCATCTTCATCGTCATAATAATGTTACTGCAGCTCCAGGTTCAGATCCTGATCCTTATGATCCTGCACTTTCACGGACGGCAGACGTCACCTTGTCACAGAATGAGACCCAGGTTAGCCATGCCTATGGTCCGTCTCTGGATGCCATTGCGGCTGCACCAAGCCATAGCCAGAAGCAGTTTGTGATATTTGTGAAGAAAGGAGTTTACAAAGAATATGCGAAGATTGATAAAACAAAGACTAACTTGGTGTTGATTGGAGAGGGCTTGAGTGTTACCACCATATCTGGAGATAGAAGCAATGCTAGTGGTTGGGCAACAATGAAGTCAGCAACATTTGCCCCACTTGATAAATGTTCCTTTGCCTGTATTCAGAAACTCTTTGGAGAAGACCACGGAACTTGTGCAAAATTGCATGCTAGCGAAGATGATGAAAATTCTACTAATATGGTCATTACATATTATGTAAGGGCCCAAGGATTCATTGCAATGGACATAGGATTTGAGAACACTGCAGGACCAAACACTACAAGTCCTACACGATTACATCCTGATCCTCAGTCTATGTTTAAGATCACTTCATCAAATTTTTTTATTTTTATTTTTTTTCATGGCTATAAGTTTGGTCAGAGGTTCAGCTTGGTTGTGAAAAACTCAACCCAATTACCCAACACACGCTTTCCTTCTTCAAAGAGCCATGCAATGACTGCCATCACCTGGGCAACAAGAAGAAGAAAAAAATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
PMEI Pectinesterase

Protein Analysis

300

Amino Acids

33.22

Weight (kDa)

9.17

Isoelectric Point (pI)

28.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 243
AccB7I CCANNNNNTGG 2 cut(s) 12, 870
AciI CCGC 2 cut(s) 86, 305
AclWI GGATC 5 cut(s) 130, 194, 200, 209, 701
AcsI RAATTY 2 cut(s) 589, 739
AcyI GRCGYC 1 cut(s) 240
AfaI GTAC 1 cut(s) 22
AfiI CCNNNNNNNGG 2 cut(s) 12, 870
AgsI TTSAA 2 cut(s) 62, 843
AjnI CCWGG 3 cut(s) 190, 264, 869
AluBI AGCT 2 cut(s) 188, 792
AluI AGCT 2 cut(s) 188, 792
Alw26I GTCTC 2 cut(s) 254, 294
AlwI GGATC 5 cut(s) 130, 194, 200, 209, 701
AlwNI CAGNNNCTG 1 cut(s) 203
AoxI GGCC 1 cut(s) 624
ApaI GGGCCC 1 cut(s) 628
ApeKI GCWGC 4 cut(s) 145, 148, 185, 308
ApoI RAATTY 2 cut(s) 589, 739
ArsI GACNNNNNNTTYG 2 cut(s) 638, 670
AspS9I GGNCC 5 cut(s) 124, 284, 624, 625, 671
AsuHPI GGTGA 2 cut(s) 235, 859
AsuNHI GCTAGC 1 cut(s) 572
AvaII GGWCC 3 cut(s) 124, 284, 671
BaeGI GKGCMC 1 cut(s) 628
BanII GRGCYC 1 cut(s) 628
BbsI GAAGAC 1 cut(s) 549
BbvI GCAGC 4 cut(s) 157, 160, 197, 295
BccI CCATC 1 cut(s) 872
BceAI ACGGC 1 cut(s) 249
BciT130I CCWGG 3 cut(s) 192, 266, 871
BcoDI GTCTC 2 cut(s) 254, 294
BfaI CTAG 2 cut(s) 459, 573
BfmI CTRYAG 2 cut(s) 183, 666
BglI GCCNNNNNGGC 1 cut(s) 305
BisI GCNGC 5 cut(s) 146, 149, 186, 306, 309
BlsI GCNGC 5 cut(s) 147, 150, 187, 307, 310
BmcAI AGTACT 1 cut(s) 22
Bme1390I CCNGG 3 cut(s) 192, 266, 871
Bme18I GGWCC 3 cut(s) 124, 284, 671
BmgT120I GGNCC 5 cut(s) 124, 284, 624, 625, 671
BmiI GGNNCC 1 cut(s) 626
BmrFI CCNGG 3 cut(s) 192, 266, 871
BmsI GCATC 1 cut(s) 286
BmtI GCTAGC 1 cut(s) 576
BpiI GAAGAC 1 cut(s) 549
BpmI CTGGAG 2 cut(s) 174, 462
BpuEI CTTGAG 1 cut(s) 442
Bsa29I ATCGAT 1 cut(s) 117
BsaBI GATNNNNATC 1 cut(s) 204
BsaHI GRCGYC 1 cut(s) 240
BsaI GGTCTC 1 cut(s) 254
BsaJI CCNNGG 5 cut(s) 127, 264, 547, 628, 870
BsaXI ACNNNNNCTCC 2 cut(s) 172, 202
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 870
Bse3DI GCAATG 5 cut(s) 300, 460, 636, 648, 861
Bse8I GATNNNNATC 1 cut(s) 204
BseBI CCWGG 3 cut(s) 192, 266, 871
BseCI ATCGAT 1 cut(s) 117
BseDI CCNNGG 5 cut(s) 127, 264, 547, 628, 870
BseGI GGATG 2 cut(s) 301, 700
BseJI GATNNNNATC 1 cut(s) 204
BseLI CCNNNNNNNGG 2 cut(s) 12, 870
BseMI GCAATG 5 cut(s) 300, 460, 636, 648, 861
BseMII CTCAG 2 cut(s) 8, 725
BseSI GKGCMC 1 cut(s) 628
BseXI GCAGC 4 cut(s) 157, 160, 197, 295
BsgI GTGCAG 3 cut(s) 164, 204, 294
BshFI GGCC 1 cut(s) 626
BshVI ATCGAT 1 cut(s) 117
BslI CCNNNNNNNGG 2 cut(s) 12, 870
BsmAI GTCTC 2 cut(s) 254, 294
BsmBI CGTCTC 1 cut(s) 294
BsnI GGCC 1 cut(s) 626
Bso31I GGTCTC 1 cut(s) 254
Bsp120I GGGCCC 1 cut(s) 624
Bsp1286I GDGCHC 1 cut(s) 628
Bsp143I GATC 7 cut(s) 69, 135, 199, 205, 214, 706, 726
BspACI CCGC 2 cut(s) 86, 305
BspANI GGCC 1 cut(s) 626
BspCNI CTCAG 2 cut(s) 9, 724
BspDI ATCGAT 1 cut(s) 117
BspLI GGNNCC 1 cut(s) 626
BspMAI CTGCAG 2 cut(s) 187, 670
BspOI GCTAGC 1 cut(s) 576
BspPI GGATC 5 cut(s) 130, 194, 200, 209, 701
BspTNI GGTCTC 1 cut(s) 254
BsrDI GCAATG 5 cut(s) 300, 460, 636, 648, 861
BssECI CCNNGG 5 cut(s) 127, 264, 547, 628, 870
BssMI GATC 7 cut(s) 69, 135, 199, 205, 214, 706, 726
BssNI GRCGYC 1 cut(s) 240
BssT1I CCWWGG 2 cut(s) 127, 628
Bst2UI CCWGG 3 cut(s) 192, 266, 871
BstACI GRCGYC 1 cut(s) 240
BstAPI GCANNNNNTGC 2 cut(s) 491, 860
BstC8I GCNNGC 2 cut(s) 570, 574
BstDEI CTNAG 3 cut(s) 17, 95, 711
BstDSI CCRYGG 1 cut(s) 547
BstF5I GGATG 2 cut(s) 301, 700
BstKTI GATC 7 cut(s) 72, 138, 202, 208, 217, 709, 729
BstMAI GTCTC 2 cut(s) 254, 294
BstMBI GATC 7 cut(s) 69, 135, 199, 205, 214, 706, 726
BstMWI GCNNNNNNNGC 3 cut(s) 305, 491, 860
BstNI CCWGG 3 cut(s) 192, 266, 871
BstNSI RCATGY 1 cut(s) 572
BstSCI CCNGG 3 cut(s) 190, 264, 869
BstSFI CTRYAG 2 cut(s) 183, 666
BstSLI GKGCMC 1 cut(s) 628
BstV1I GCAGC 4 cut(s) 157, 160, 197, 295
BstV2I GAAGAC 1 cut(s) 549
BstX2I RGATCY 1 cut(s) 199
BstXI CCANNNNNNTGG 1 cut(s) 281
BstYI RGATCY 1 cut(s) 199
Bsu15I ATCGAT 1 cut(s) 117
BsuRI GGCC 1 cut(s) 626
BsuTUI ATCGAT 1 cut(s) 117
BtgI CCRYGG 1 cut(s) 547
BtsCI GGATG 2 cut(s) 301, 700
BtsI GCAGTG 1 cut(s) 663
BtsIMutI CAGTG 1 cut(s) 663
Cac8I GCNNGC 2 cut(s) 570, 574
CaiI CAGNNNCTG 1 cut(s) 203
Cfr13I GGNCC 5 cut(s) 124, 284, 624, 625, 671
ClaI ATCGAT 1 cut(s) 117
Csp6I GTAC 1 cut(s) 21
CspCI CAANNNNNGTGG 4 cut(s) 442, 477, 536, 571
CviAII CATG 5 cut(s) 12, 275, 569, 764, 851
CviQI GTAC 1 cut(s) 21
DdeI CTNAG 3 cut(s) 17, 95, 711
DpnI GATC 7 cut(s) 71, 137, 201, 207, 216, 708, 728
DpnII GATC 7 cut(s) 69, 135, 199, 205, 214, 706, 726
Eco130I CCWWGG 2 cut(s) 127, 628
Eco24I GRGCYC 1 cut(s) 628
Eco31I GGTCTC 1 cut(s) 254
Eco47I GGWCC 3 cut(s) 124, 284, 671
EcoO109I RGGNCCY 2 cut(s) 124, 624
EcoRII CCWGG 3 cut(s) 190, 264, 869
EcoT14I CCWWGG 2 cut(s) 127, 628
EcoT38I GRGCYC 1 cut(s) 628
ErhI CCWWGG 2 cut(s) 127, 628
Esp3I CGTCTC 1 cut(s) 294
FaeI CATG 5 cut(s) 15, 278, 572, 767, 854
FalI AAGNNNNNCTT 2 cut(s) 383, 415
FatI CATG 5 cut(s) 11, 274, 568, 763, 850
Fnu4HI GCNGC 5 cut(s) 146, 149, 186, 306, 309
FokI GGATG 2 cut(s) 308, 687
FriOI GRGCYC 1 cut(s) 628
Fsp4HI GCNGC 5 cut(s) 146, 149, 186, 306, 309
FspBI CTAG 2 cut(s) 459, 573
GluI GCNGC 5 cut(s) 146, 149, 186, 306, 309
GsuI CTGGAG 2 cut(s) 174, 462
HaeIII GGCC 1 cut(s) 626
Hin1I GRCGYC 1 cut(s) 240
Hin1II CATG 5 cut(s) 15, 278, 572, 767, 854
HinfI GANTC 2 cut(s) 74, 633
HphI GGTGA 2 cut(s) 235, 859
Hpy166II GTNNAC 2 cut(s) 52, 361
Hpy188I TCNGA 3 cut(s) 199, 528, 783
Hpy188III TCNNGA 4 cut(s) 203, 293, 441, 704
Hpy8I GTNNAC 2 cut(s) 52, 361
HpyAV CCTTC 1 cut(s) 846
HpyCH4IV ACGT 1 cut(s) 240
HpyCH4V TGCA 9 cut(s) 145, 185, 221, 311, 560, 568, 641, 668, 854
HpyF10VI GCNNNNNNNGC 3 cut(s) 305, 491, 860
HpyF3I CTNAG 3 cut(s) 17, 95, 711
HpySE526I ACGT 1 cut(s) 240
Hsp92I GRCGYC 1 cut(s) 240
Hsp92II CATG 5 cut(s) 15, 278, 572, 767, 854
Kzo9I GATC 7 cut(s) 69, 135, 199, 205, 214, 706, 726
LmnI GCTCC 1 cut(s) 193
Lsp1109I GCAGC 4 cut(s) 157, 160, 197, 295
LweI GCATC 1 cut(s) 286
MaeI CTAG 2 cut(s) 459, 573
MaeII ACGT 1 cut(s) 240
MaeIII GTNAC 5 cut(s) 103, 178, 241, 249, 427
MalI GATC 7 cut(s) 71, 137, 201, 207, 216, 708, 728
MboI GATC 7 cut(s) 69, 135, 199, 205, 214, 706, 726
MboII GAAGA 8 cut(s) 152, 361, 388, 554, 590, 831, 894, 897
MflI RGATCY 1 cut(s) 199
MhlI GDGCHC 1 cut(s) 628
MluCI AATT 5 cut(s) 563, 589, 739, 815, 893
MlyI GAGTC 1 cut(s) 68
MnlI CCTC 4 cut(s) 115, 409, 720, 777
MseI TTAA 1 cut(s) 723
MslI CAYNNNNRTG 3 cut(s) 174, 279, 641
MspR9I CCNGG 3 cut(s) 192, 266, 871
MvaI CCWGG 3 cut(s) 192, 266, 871
MwoI GCNNNNNNNGC 3 cut(s) 305, 491, 860
NdeII GATC 7 cut(s) 69, 135, 199, 205, 214, 706, 726
NheI GCTAGC 1 cut(s) 572
NlaIII CATG 5 cut(s) 15, 278, 572, 767, 854
NlaIV GGNNCC 1 cut(s) 626
NmuCI GTSAC 3 cut(s) 103, 241, 249
NspI RCATGY 1 cut(s) 572
PaeI GCATGC 1 cut(s) 572
PfeI GAWTC 1 cut(s) 633
PflMI CCANNNNNTGG 2 cut(s) 12, 870
PkrI GCNGC 5 cut(s) 147, 150, 187, 307, 310
PleI GAGTC 1 cut(s) 68
PpsI GAGTC 1 cut(s) 68
PpuMI RGGWCCY 1 cut(s) 124
Psp5II RGGWCCY 1 cut(s) 124
Psp6I CCWGG 3 cut(s) 190, 264, 869
PspGI CCWGG 3 cut(s) 190, 264, 869
PspN4I GGNNCC 1 cut(s) 626
PspOMI GGGCCC 1 cut(s) 624
PspPI GGNCC 5 cut(s) 124, 284, 624, 625, 671
PspPPI RGGWCCY 1 cut(s) 124
PstI CTGCAG 2 cut(s) 187, 670
PstNI CAGNNNCTG 1 cut(s) 203
PsuI RGATCY 1 cut(s) 199
RsaI GTAC 1 cut(s) 22
RsaNI GTAC 1 cut(s) 21
RseI CAYNNNNRTG 3 cut(s) 174, 279, 641
SaqAI TTAA 1 cut(s) 723
SatI GCNGC 5 cut(s) 146, 149, 186, 306, 309
Sau3AI GATC 7 cut(s) 69, 135, 199, 205, 214, 706, 726
Sau96I GGNCC 5 cut(s) 124, 284, 624, 625, 671
ScaI AGTACT 1 cut(s) 22
SchI GAGTC 1 cut(s) 68
ScrFI CCNGG 3 cut(s) 192, 266, 871
SduI GDGCHC 1 cut(s) 628
SetI ASST 9 cut(s) 126, 190, 196, 243, 248, 270, 788, 794, 872
SfaNI GCATC 1 cut(s) 286
SfcI CTRYAG 2 cut(s) 183, 666
SinI GGWCC 3 cut(s) 124, 284, 671
SmiMI CAYNNNNRTG 3 cut(s) 174, 279, 641
SmlI CTYRAG 1 cut(s) 421
SmoI CTYRAG 1 cut(s) 421
SphI GCATGC 1 cut(s) 572
Sse9I AATT 5 cut(s) 563, 589, 739, 815, 893
SsiI CCGC 2 cut(s) 86, 305
SspMI CTAG 2 cut(s) 459, 573
StyD4I CCNGG 3 cut(s) 190, 264, 869
StyI CCWWGG 2 cut(s) 127, 628
TaiI ACGT 1 cut(s) 243
TaqI TCGA 2 cut(s) 72, 117
TasI AATT 5 cut(s) 563, 589, 739, 815, 893
TatI WGTACW 1 cut(s) 20
TauI GCSGC 1 cut(s) 308
TfiI GAWTC 1 cut(s) 633
Tru1I TTAA 1 cut(s) 723
Tru9I TTAA 1 cut(s) 723
TscAI CASTG 1 cut(s) 670
TseFI GTSAC 3 cut(s) 103, 241, 249
TseI GCWGC 4 cut(s) 145, 148, 185, 308
Tsp45I GTSAC 3 cut(s) 103, 241, 249
TspDTI ATGAA 6 cut(s) 152, 491, 600, 625, 723, 752
TspGWI ACGGA 3 cut(s) 244, 276, 564
TspRI CASTG 1 cut(s) 670
Van91I CCANNNNNTGG 2 cut(s) 12, 870
VpaK11BI GGWCC 3 cut(s) 124, 284, 671
XapI RAATTY 2 cut(s) 589, 739
XceI RCATGY 1 cut(s) 572
XspI CTAG 2 cut(s) 459, 573
ZraI GACGTC 1 cut(s) 241
ZrmI AGTACT 1 cut(s) 22
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.