Rh1AG311400

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0001935
Physical Location & Seq
Forward (+)
54494621 .. 54495529
909 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG311400.1

Sequence Viewer

Length: 648 bp
ATGTTGCCTGAGCTGCCTAATGCTCTTGATCTAGAACCTAGATCACATCAGACTCAGTTCCAGCAAATCAGTTATTGGAAGAATAACCCTAACGTCATCGTGTCTAGGGATTGGACTGGAAATTTCCAAAAGATAATGCAGGCAGTAGAGGCTGTACCGGATCATAGCCGGAAGCGTCTTGTGATCTTGGTGAAGAGAGGAGTTTACAAGGAAAATGTGGATATCAATGTGAAAAAGACTAATTTGGTTTTGATTGGAGAGGGCATGGACGTTACTACCATAACTGGACATAAGAGTAATGGTACTGGTTGGCGTACATTTACCTCGGCGACATCTACTGTGAAGGCAGAAGGCTTCATTGCAATCGATATAGGGTTTGAGAACACAGCCGGACCACACAACGGCCAGGCAGTGGCACTTCTAGTCAAGGGTGACCAATCAGCTTTCTTCCGATGCAAGATAAGTGGATACCAAGTCACATTGTATGCACACAACAGCCGCCAGTTCTTTAGGGAATGCAAGATCAGTGGCACTGTCGACTTCATCTTCGGCTACAGCACTGCAGTTTTCCAAATGTGCCCCAATATTGCGAAGAAAAGACCTAATCATGGCCTGGTTACAGTTACTGCCCACGGTCGTTTCGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
PMEI Pectinesterase

Protein Analysis

215

Amino Acids

24.03

Weight (kDa)

9.51

Isoelectric Point (pI)

20.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 31 - 213 2.8e-72 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 412
AccI GTMKAC 1 cut(s) 537
AciI CCGC 1 cut(s) 499
AclWI GGATC 1 cut(s) 168
AcoI YGGCCR 1 cut(s) 403
AcsI RAATTY 1 cut(s) 121
AfaI GTAC 3 cut(s) 156, 304, 316
AfiI CCNNNNNNNGG 3 cut(s) 401, 412, 608
AjnI CCWGG 2 cut(s) 405, 612
AluBI AGCT 2 cut(s) 13, 443
AluI AGCT 2 cut(s) 13, 443
AlwI GGATC 1 cut(s) 168
AlwNI CAGNNNCTG 1 cut(s) 626
AoxI GGCC 2 cut(s) 403, 610
ApeKI GCWGC 1 cut(s) 13
ApoI RAATTY 1 cut(s) 121
ArsI GACNNNNNNTTYG 2 cut(s) 530, 562
AspS9I GGNCC 1 cut(s) 392
AsuHPI GGTGA 2 cut(s) 202, 443
AsuII TTCGAA 1 cut(s) 642
AvaII GGWCC 1 cut(s) 392
BaeGI GKGCMC 1 cut(s) 581
BceAI ACGGC 1 cut(s) 418
BciT130I CCWGG 2 cut(s) 407, 614
BciVI GTATCC 1 cut(s) 461
BfaI CTAG 4 cut(s) 32, 39, 105, 422
BfmI CTRYAG 2 cut(s) 553, 561
BfuI GTATCC 1 cut(s) 461
BisI GCNGC 2 cut(s) 14, 499
BlsI GCNGC 2 cut(s) 15, 500
Bme1390I CCNGG 2 cut(s) 407, 614
Bme18I GGWCC 1 cut(s) 392
BmgT120I GGNCC 1 cut(s) 392
BmrFI CCNGG 2 cut(s) 407, 614
BmsI GCATC 1 cut(s) 443
Bpu10I CCTNAGC 1 cut(s) 9
Bpu14I TTCGAA 1 cut(s) 642
Bsa29I ATCGAT 1 cut(s) 366
BsaJI CCNNGG 2 cut(s) 324, 631
BsaWI WCCGGW 1 cut(s) 157
Bsc4I CCNNNNNNNGG 3 cut(s) 401, 412, 608
Bse1I ACTGG 4 cut(s) 121, 289, 310, 502
Bse3DI GCAATG 1 cut(s) 357
BseBI CCWGG 2 cut(s) 407, 614
BseCI ATCGAT 1 cut(s) 366
BseDI CCNNGG 2 cut(s) 324, 631
BseLI CCNNNNNNNGG 3 cut(s) 401, 412, 608
BseMI GCAATG 1 cut(s) 357
BseMII CTCAG 1 cut(s) 68
BseNI ACTGG 4 cut(s) 121, 289, 310, 502
BseRI GAGGAG 1 cut(s) 213
BseSI GKGCMC 1 cut(s) 581
Bsh1285I CGRYCG 1 cut(s) 637
BshFI GGCC 2 cut(s) 405, 612
BshVI ATCGAT 1 cut(s) 366
BsiEI CGRYCG 1 cut(s) 637
BsiSI CCGG 3 cut(s) 158, 169, 390
BslI CCNNNNNNNGG 3 cut(s) 401, 412, 608
BsmI GAATGC 1 cut(s) 521
BsnI GGCC 2 cut(s) 405, 612
Bsp119I TTCGAA 1 cut(s) 642
Bsp1286I GDGCHC 1 cut(s) 581
Bsp143I GATC 5 cut(s) 28, 41, 160, 183, 522
BspACI CCGC 1 cut(s) 499
BspANI GGCC 2 cut(s) 405, 612
BspCNI CTCAG 1 cut(s) 67
BspDI ATCGAT 1 cut(s) 366
BspMAI CTGCAG 1 cut(s) 565
BspPI GGATC 1 cut(s) 168
BspT104I TTCGAA 1 cut(s) 642
BsrDI GCAATG 1 cut(s) 357
BsrI ACTGG 4 cut(s) 121, 289, 310, 502
BssECI CCNNGG 2 cut(s) 324, 631
BssMI GATC 5 cut(s) 28, 41, 160, 183, 522
Bst2UI CCWGG 2 cut(s) 407, 614
Bst4CI ACNGT 4 cut(s) 340, 535, 622, 635
Bst6I CTCTTC 1 cut(s) 188
BstBI TTCGAA 1 cut(s) 642
BstC8I GCNNGC 1 cut(s) 141
BstDEI CTNAG 2 cut(s) 9, 54
BstDSI CCRYGG 1 cut(s) 631
BstEII GGTNACC 1 cut(s) 431
BstKTI GATC 5 cut(s) 31, 44, 163, 186, 525
BstMBI GATC 5 cut(s) 28, 41, 160, 183, 522
BstMCI CGRYCG 1 cut(s) 637
BstMWI GCNNNNNNNGC 2 cut(s) 13, 149
BstNI CCWGG 2 cut(s) 407, 614
BstPI GGTNACC 1 cut(s) 431
BstSCI CCNGG 2 cut(s) 405, 612
BstSFI CTRYAG 2 cut(s) 553, 561
BstSLI GKGCMC 1 cut(s) 581
Bsu15I ATCGAT 1 cut(s) 366
BsuI GTATCC 1 cut(s) 461
BsuRI GGCC 2 cut(s) 405, 612
BsuTUI ATCGAT 1 cut(s) 366
BtgI CCRYGG 1 cut(s) 631
BtsI GCAGTG 2 cut(s) 417, 558
BtsIMutI CAGTG 4 cut(s) 417, 531, 532, 558
Cac8I GCNNGC 1 cut(s) 141
CaiI CAGNNNCTG 1 cut(s) 626
Cfr13I GGNCC 1 cut(s) 392
ClaI ATCGAT 1 cut(s) 366
CseI GACGC 1 cut(s) 164
Csp6I GTAC 3 cut(s) 155, 303, 315
CspCI CAANNNNNGTGG 4 cut(s) 445, 480, 508, 543
CviAII CATG 2 cut(s) 265, 608
CviQI GTAC 3 cut(s) 155, 303, 315
DdeI CTNAG 2 cut(s) 9, 54
DpnI GATC 5 cut(s) 30, 43, 162, 185, 524
DpnII GATC 5 cut(s) 28, 41, 160, 183, 522
EaeI YGGCCR 1 cut(s) 403
Eam1104I CTCTTC 1 cut(s) 188
EarI CTCTTC 1 cut(s) 188
Eco32I GATATC 1 cut(s) 223
Eco47I GGWCC 1 cut(s) 392
Eco91I GGTNACC 1 cut(s) 431
EcoO65I GGTNACC 1 cut(s) 431
EcoRII CCWGG 2 cut(s) 405, 612
EcoRV GATATC 1 cut(s) 223
FaeI CATG 2 cut(s) 268, 611
FaiI YATR 7 cut(s) 165, 266, 281, 291, 371, 486, 609
FatI CATG 2 cut(s) 264, 607
FblI GTMKAC 1 cut(s) 537
Fnu4HI GCNGC 2 cut(s) 14, 499
Fsp4HI GCNGC 2 cut(s) 14, 499
FspBI CTAG 4 cut(s) 32, 39, 105, 422
GluI GCNGC 2 cut(s) 14, 499
HaeIII GGCC 2 cut(s) 405, 612
HapII CCGG 3 cut(s) 158, 169, 390
HgaI GACGC 1 cut(s) 164
Hin1II CATG 2 cut(s) 268, 611
HincII GTYRAC 1 cut(s) 538
HindII GTYRAC 1 cut(s) 538
HinfI GANTC 1 cut(s) 52
HpaII CCGG 3 cut(s) 158, 169, 390
HphI GGTGA 2 cut(s) 202, 443
Hpy166II GTNNAC 2 cut(s) 205, 538
Hpy188I TCNGA 2 cut(s) 51, 452
Hpy188III TCNNGA 2 cut(s) 26, 32
Hpy8I GTNNAC 2 cut(s) 205, 538
HpyAV CCTTC 2 cut(s) 337, 344
HpyCH4III ACNGT 4 cut(s) 340, 535, 622, 635
HpyCH4IV ACGT 2 cut(s) 93, 270
HpyCH4V TGCA 6 cut(s) 139, 362, 456, 488, 519, 563
HpyF10VI GCNNNNNNNGC 2 cut(s) 13, 149
HpyF3I CTNAG 2 cut(s) 9, 54
HpySE526I ACGT 2 cut(s) 93, 270
Hsp92II CATG 2 cut(s) 268, 611
Kzo9I GATC 5 cut(s) 28, 41, 160, 183, 522
LweI GCATC 1 cut(s) 443
MaeI CTAG 4 cut(s) 32, 39, 105, 422
MaeII ACGT 2 cut(s) 93, 270
MaeIII GTNAC 5 cut(s) 271, 431, 475, 616, 622
MalI GATC 5 cut(s) 30, 43, 162, 185, 524
MboI GATC 5 cut(s) 28, 41, 160, 183, 522
MboII GAAGA 5 cut(s) 91, 205, 439, 538, 604
MhlI GDGCHC 1 cut(s) 581
MluCI AATT 2 cut(s) 121, 241
MlyI GAGTC 1 cut(s) 46
MnlI CCTC 4 cut(s) 142, 191, 253, 334
MspI CCGG 3 cut(s) 158, 169, 390
MspR9I CCNGG 2 cut(s) 407, 614
Mva1269I GAATGC 1 cut(s) 521
MvaI CCWGG 2 cut(s) 407, 614
MwoI GCNNNNNNNGC 2 cut(s) 13, 149
NdeII GATC 5 cut(s) 28, 41, 160, 183, 522
NlaIII CATG 2 cut(s) 268, 611
NmeAIII GCCGAG 1 cut(s) 305
NmuCI GTSAC 2 cut(s) 431, 475
NspV TTCGAA 1 cut(s) 642
PcsI WCGNNNNNNNCGW 1 cut(s) 639
PctI GAATGC 1 cut(s) 521
PflMI CCANNNNNTGG 1 cut(s) 412
PkrI GCNGC 2 cut(s) 15, 500
PleI GAGTC 1 cut(s) 46
PpsI GAGTC 1 cut(s) 46
Psp6I CCWGG 2 cut(s) 405, 612
PspEI GGTNACC 1 cut(s) 431
PspGI CCWGG 2 cut(s) 405, 612
PspPI GGNCC 1 cut(s) 392
PstI CTGCAG 1 cut(s) 565
PstNI CAGNNNCTG 1 cut(s) 626
RsaI GTAC 3 cut(s) 156, 304, 316
RsaNI GTAC 3 cut(s) 155, 303, 315
SalI GTCGAC 1 cut(s) 536
SatI GCNGC 2 cut(s) 14, 499
Sau3AI GATC 5 cut(s) 28, 41, 160, 183, 522
Sau96I GGNCC 1 cut(s) 392
SchI GAGTC 1 cut(s) 46
ScrFI CCNGG 2 cut(s) 407, 614
SduI GDGCHC 1 cut(s) 581
SetI ASST 7 cut(s) 15, 40, 96, 273, 326, 445, 604
SfaNI GCATC 1 cut(s) 443
SfcI CTRYAG 2 cut(s) 553, 561
SfuI TTCGAA 1 cut(s) 642
SinI GGWCC 1 cut(s) 392
Sse9I AATT 2 cut(s) 121, 241
SsiI CCGC 1 cut(s) 499
SspI AATATT 1 cut(s) 586
SspMI CTAG 4 cut(s) 32, 39, 105, 422
StyD4I CCNGG 2 cut(s) 405, 612
TaaI ACNGT 4 cut(s) 340, 535, 622, 635
TaiI ACGT 2 cut(s) 96, 273
TaqI TCGA 3 cut(s) 366, 537, 642
TasI AATT 2 cut(s) 121, 241
TauI GCSGC 1 cut(s) 501
TscAI CASTG 4 cut(s) 417, 532, 538, 565
TseFI GTSAC 2 cut(s) 431, 475
TseI GCWGC 1 cut(s) 13
Tsp45I GTSAC 2 cut(s) 431, 475
TspDTI ATGAA 2 cut(s) 346, 532
TspRI CASTG 4 cut(s) 417, 532, 538, 565
Van91I CCANNNNNTGG 1 cut(s) 412
VpaK11BI GGWCC 1 cut(s) 392
XapI RAATTY 1 cut(s) 121
XbaI TCTAGA 1 cut(s) 31
XmiI GTMKAC 1 cut(s) 537
XspI CTAG 4 cut(s) 32, 39, 105, 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.