Rw1G027600

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0001935
Physical Location & Seq
Forward (+)
55132960 .. 55134106
1147 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G027600.1

Sequence Viewer

Length: 987 bp
ATGTTGCCTGAGCTGCCTAATGCTCTTGATCTAGAACCTAGATCACATCAGACTCAGTTCCAGCAAATCAGTTATTGGAAGAATAACCCTAACGTCATCGTGTCTAGGGATTGGACTGGAAATTTCCAAAAGATAATGCAGGCAGTAGAGGCTGTACCGGATCATAGCCGGAAGCGTCTTGTGATCTTGGTGAAGAGAGGAGTTTACAAGGAAAATGTGGATATCAATGTGAAAAAGACTAATTTGGTTTTGATTGGAGAGGGCATGGACGTTACTACCATAACTGGACATAAGAGTAATGGTACTGGTTGGCGTACATTTACCTCGGCGACATCTACTGTGAAGGCAGAAGGCTTCATTGCAATCGATATAGGGTTTGAGAACACAGCCGGACCACACAACGGCCAGGCAGTGGCACTTCTAGTCAAGGGTGACCAATCAGCTTTCTTCCGATGCAAGATAAGTGGATACCAAGTCACATTGTATGCACACAACAGCCGCCAGTTCTTTAGGGAATGCAAGATCAGTGGCACTGTCGACTTCATCTTCGGCTACAGCACTGCAGTTTTCCAAATGTGCCCCAATATTGCGAAGAAAAGACCTAATCATGGCCTGGTTACAGTTACTGCCCACTGTCATGCTGATTTGTGGAGCAACATTACAGCTACACCCACATACTTGGGTCGACGGGGTAATTACTCACGTACAGTTTTCATGCAATCGACCCTAGGTAGTGTAGTAACTCCCAAGGGTTGGATTGAATGGAATGGAAACCGTGGCATTGACACTCTGTATTATGGTGAGTTCATGAATAAAGGACCAGGTTCCGAGCTCGCAGGGCGGCTCAGATGGCTCGGTCATCATACTGATCCATCTGAGGCCGATCAGTTTACGGCCACCAACTTTATTGACGGAGATTTTTGGCTGCCATCCACAGGTATCCCATACACATCAGAATTGGGGAGTCCAATACCTCAACCTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
PMEI Pectinesterase

Protein Analysis

328

Amino Acids

36.51

Weight (kDa)

8.95

Isoelectric Point (pI)

29.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 31 - 215 4.7e-71 Pectinesterase
Pectinesterase PF01095 215 - 306 1.3e-28 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 412, 753
AccI GTMKAC 2 cut(s) 537, 685
AciI CCGC 2 cut(s) 499, 841
AclWI GGATC 2 cut(s) 168, 863
AcoI YGGCCR 2 cut(s) 403, 894
AcsI RAATTY 1 cut(s) 121
AfaI GTAC 4 cut(s) 156, 304, 316, 706
AfiI CCNNNNNNNGG 5 cut(s) 401, 412, 608, 753, 935
AgsI TTSAA 1 cut(s) 761
AjnI CCWGG 3 cut(s) 405, 612, 820
AluBI AGCT 5 cut(s) 13, 443, 665, 832, 984
AluI AGCT 5 cut(s) 13, 443, 665, 832, 984
Alw21I GWGCWC 1 cut(s) 834
AlwI GGATC 2 cut(s) 168, 863
AlwNI CAGNNNCTG 1 cut(s) 626
AoxI GGCC 4 cut(s) 403, 610, 879, 894
ApeKI GCWGC 2 cut(s) 13, 925
ApoI RAATTY 1 cut(s) 121
ArsI GACNNNNNNTTYG 2 cut(s) 530, 562
AspA2I CCTAGG 1 cut(s) 727
AspS9I GGNCC 2 cut(s) 392, 818
AsuHPI GGTGA 3 cut(s) 202, 443, 812
AvaII GGWCC 2 cut(s) 392, 818
AvrII CCTAGG 1 cut(s) 727
BaeGI GKGCMC 1 cut(s) 581
BanII GRGCYC 1 cut(s) 834
Bbv12I GWGCWC 1 cut(s) 834
BbvI GCAGC 1 cut(s) 912
BccI CCATC 3 cut(s) 843, 880, 937
BceAI ACGGC 2 cut(s) 418, 909
BciT130I CCWGG 3 cut(s) 407, 614, 822
BciVI GTATCC 2 cut(s) 461, 950
BfaI CTAG 6 cut(s) 32, 39, 105, 422, 728, 981
BfmI CTRYAG 2 cut(s) 553, 561
BfuI GTATCC 2 cut(s) 461, 950
BisI GCNGC 4 cut(s) 14, 499, 842, 926
BlnI CCTAGG 1 cut(s) 727
BlsI GCNGC 4 cut(s) 15, 500, 843, 927
Bme1390I CCNGG 3 cut(s) 407, 614, 822
Bme18I GGWCC 2 cut(s) 392, 818
BmgT120I GGNCC 2 cut(s) 392, 818
BmiI GGNNCC 1 cut(s) 826
BmrFI CCNGG 3 cut(s) 407, 614, 822
BmsI GCATC 1 cut(s) 443
Bpu10I CCTNAGC 1 cut(s) 9
Bsa29I ATCGAT 1 cut(s) 366
BsaAI YACGTR 1 cut(s) 704
BsaJI CCNNGG 4 cut(s) 324, 727, 747, 775
BsaWI WCCGGW 1 cut(s) 157
Bsc4I CCNNNNNNNGG 5 cut(s) 401, 412, 608, 753, 935
Bse1I ACTGG 4 cut(s) 121, 289, 310, 502
Bse3DI GCAATG 1 cut(s) 357
BseBI CCWGG 3 cut(s) 407, 614, 822
BseCI ATCGAT 1 cut(s) 366
BseDI CCNNGG 4 cut(s) 324, 727, 747, 775
BseGI GGATG 1 cut(s) 929
BseLI CCNNNNNNNGG 5 cut(s) 401, 412, 608, 753, 935
BseMI GCAATG 1 cut(s) 357
BseMII CTCAG 3 cut(s) 68, 859, 867
BseNI ACTGG 4 cut(s) 121, 289, 310, 502
BseRI GAGGAG 1 cut(s) 213
BseSI GKGCMC 1 cut(s) 581
BseXI GCAGC 1 cut(s) 912
BshFI GGCC 4 cut(s) 405, 612, 881, 896
BshVI ATCGAT 1 cut(s) 366
BsiHKAI GWGCWC 1 cut(s) 834
BsiSI CCGG 3 cut(s) 158, 169, 390
BslI CCNNNNNNNGG 5 cut(s) 401, 412, 608, 753, 935
BsmI GAATGC 1 cut(s) 521
BsnI GGCC 4 cut(s) 405, 612, 881, 896
Bsp1286I GDGCHC 2 cut(s) 581, 834
Bsp143I GATC 7 cut(s) 28, 41, 160, 183, 522, 868, 883
BspACI CCGC 2 cut(s) 499, 841
BspANI GGCC 4 cut(s) 405, 612, 881, 896
BspCNI CTCAG 3 cut(s) 67, 858, 868
BspDI ATCGAT 1 cut(s) 366
BspHI TCATGA 1 cut(s) 807
BspLI GGNNCC 1 cut(s) 826
BspMAI CTGCAG 1 cut(s) 565
BspPI GGATC 2 cut(s) 168, 863
BsrDI GCAATG 1 cut(s) 357
BsrI ACTGG 4 cut(s) 121, 289, 310, 502
BssECI CCNNGG 4 cut(s) 324, 727, 747, 775
BssMI GATC 7 cut(s) 28, 41, 160, 183, 522, 868, 883
BssT1I CCWWGG 2 cut(s) 727, 747
Bst2UI CCWGG 3 cut(s) 407, 614, 822
Bst4CI ACNGT 6 cut(s) 340, 535, 622, 635, 709, 776
Bst6I CTCTTC 1 cut(s) 188
BstBAI YACGTR 1 cut(s) 704
BstC8I GCNNGC 2 cut(s) 141, 834
BstDEI CTNAG 4 cut(s) 9, 54, 845, 876
BstDSI CCRYGG 1 cut(s) 775
BstEII GGTNACC 1 cut(s) 431
BstF5I GGATG 1 cut(s) 929
BstKTI GATC 7 cut(s) 31, 44, 163, 186, 525, 871, 886
BstMBI GATC 7 cut(s) 28, 41, 160, 183, 522, 868, 883
BstMWI GCNNNNNNNGC 4 cut(s) 13, 149, 838, 850
BstNI CCWGG 3 cut(s) 407, 614, 822
BstPI GGTNACC 1 cut(s) 431
BstSCI CCNGG 3 cut(s) 405, 612, 820
BstSFI CTRYAG 2 cut(s) 553, 561
BstSLI GKGCMC 1 cut(s) 581
BstV1I GCAGC 1 cut(s) 912
BstXI CCANNNNNNTGG 1 cut(s) 679
Bsu15I ATCGAT 1 cut(s) 366
BsuI GTATCC 2 cut(s) 461, 950
BsuRI GGCC 4 cut(s) 405, 612, 881, 896
BsuTUI ATCGAT 1 cut(s) 366
BtgI CCRYGG 1 cut(s) 775
BtsCI GGATG 1 cut(s) 929
BtsI GCAGTG 2 cut(s) 417, 558
BtsIMutI CAGTG 5 cut(s) 417, 531, 532, 558, 631
Cac8I GCNNGC 2 cut(s) 141, 834
CaiI CAGNNNCTG 1 cut(s) 626
CciI TCATGA 1 cut(s) 807
Cfr13I GGNCC 2 cut(s) 392, 818
ClaI ATCGAT 1 cut(s) 366
CseI GACGC 1 cut(s) 164
CsiI ACCWGGT 1 cut(s) 820
Csp6I GTAC 4 cut(s) 155, 303, 315, 705
CspCI CAANNNNNGTGG 4 cut(s) 445, 480, 508, 543
CviAII CATG 5 cut(s) 265, 608, 638, 715, 808
CviQI GTAC 4 cut(s) 155, 303, 315, 705
DdeI CTNAG 4 cut(s) 9, 54, 845, 876
DpnI GATC 7 cut(s) 30, 43, 162, 185, 524, 870, 885
DpnII GATC 7 cut(s) 28, 41, 160, 183, 522, 868, 883
EaeI YGGCCR 2 cut(s) 403, 894
Eam1104I CTCTTC 1 cut(s) 188
EarI CTCTTC 1 cut(s) 188
Ecl136II GAGCTC 1 cut(s) 832
Eco130I CCWWGG 2 cut(s) 727, 747
Eco24I GRGCYC 1 cut(s) 834
Eco32I GATATC 1 cut(s) 223
Eco47I GGWCC 2 cut(s) 392, 818
Eco53kI GAGCTC 1 cut(s) 832
Eco91I GGTNACC 1 cut(s) 431
EcoICRI GAGCTC 1 cut(s) 832
EcoO65I GGTNACC 1 cut(s) 431
EcoRII CCWGG 3 cut(s) 405, 612, 820
EcoRV GATATC 1 cut(s) 223
EcoT14I CCWWGG 2 cut(s) 727, 747
EcoT38I GRGCYC 1 cut(s) 834
ErhI CCWWGG 2 cut(s) 727, 747
FaeI CATG 5 cut(s) 268, 611, 641, 718, 811
FatI CATG 5 cut(s) 264, 607, 637, 714, 807
FblI GTMKAC 2 cut(s) 537, 685
Fnu4HI GCNGC 4 cut(s) 14, 499, 842, 926
FokI GGATG 1 cut(s) 916
FriOI GRGCYC 1 cut(s) 834
Fsp4HI GCNGC 4 cut(s) 14, 499, 842, 926
FspBI CTAG 6 cut(s) 32, 39, 105, 422, 728, 981
GluI GCNGC 4 cut(s) 14, 499, 842, 926
HaeIII GGCC 4 cut(s) 405, 612, 881, 896
HapII CCGG 3 cut(s) 158, 169, 390
HgaI GACGC 1 cut(s) 164
Hin1II CATG 5 cut(s) 268, 611, 641, 718, 811
HincII GTYRAC 2 cut(s) 538, 686
HindII GTYRAC 2 cut(s) 538, 686
HinfI GANTC 2 cut(s) 52, 964
HpaII CCGG 3 cut(s) 158, 169, 390
HphI GGTGA 3 cut(s) 202, 443, 812
Hpy166II GTNNAC 4 cut(s) 205, 538, 686, 891
Hpy188I TCNGA 6 cut(s) 51, 452, 829, 848, 877, 955
Hpy188III TCNNGA 3 cut(s) 26, 32, 808
Hpy8I GTNNAC 4 cut(s) 205, 538, 686, 891
Hpy99I CGWCG 1 cut(s) 690
HpyAV CCTTC 2 cut(s) 337, 344
HpyCH4III ACNGT 6 cut(s) 340, 535, 622, 635, 709, 776
HpyCH4IV ACGT 3 cut(s) 93, 270, 703
HpyCH4V TGCA 7 cut(s) 139, 362, 456, 488, 519, 563, 718
HpyF10VI GCNNNNNNNGC 4 cut(s) 13, 149, 838, 850
HpyF3I CTNAG 4 cut(s) 9, 54, 845, 876
HpySE526I ACGT 3 cut(s) 93, 270, 703
Hsp92II CATG 5 cut(s) 268, 611, 641, 718, 811
Kzo9I GATC 7 cut(s) 28, 41, 160, 183, 522, 868, 883
LmnI GCTCC 1 cut(s) 651
Lsp1109I GCAGC 1 cut(s) 912
LweI GCATC 1 cut(s) 443
MabI ACCWGGT 1 cut(s) 820
MaeI CTAG 6 cut(s) 32, 39, 105, 422, 728, 981
MaeII ACGT 3 cut(s) 93, 270, 703
MaeIII GTNAC 6 cut(s) 271, 431, 475, 616, 622, 739
MalI GATC 7 cut(s) 30, 43, 162, 185, 524, 870, 885
MboI GATC 7 cut(s) 28, 41, 160, 183, 522, 868, 883
MboII GAAGA 5 cut(s) 91, 205, 439, 538, 604
MhlI GDGCHC 2 cut(s) 581, 834
MluCI AATT 4 cut(s) 121, 241, 694, 956
MlyI GAGTC 2 cut(s) 46, 973
MmeI TCCRAC 1 cut(s) 734
MnlI CCTC 6 cut(s) 142, 191, 253, 334, 871, 984
MslI CAYNNNNRTG 1 cut(s) 636
MspI CCGG 3 cut(s) 158, 169, 390
MspR9I CCNGG 3 cut(s) 407, 614, 822
Mva1269I GAATGC 1 cut(s) 521
MvaI CCWGG 3 cut(s) 407, 614, 822
MwoI GCNNNNNNNGC 4 cut(s) 13, 149, 838, 850
NdeII GATC 7 cut(s) 28, 41, 160, 183, 522, 868, 883
NlaIII CATG 5 cut(s) 268, 611, 641, 718, 811
NlaIV GGNNCC 1 cut(s) 826
NmeAIII GCCGAG 1 cut(s) 305
NmuCI GTSAC 2 cut(s) 431, 475
PagI TCATGA 1 cut(s) 807
PctI GAATGC 1 cut(s) 521
PflMI CCANNNNNTGG 2 cut(s) 412, 753
PkrI GCNGC 4 cut(s) 15, 500, 843, 927
PleI GAGTC 2 cut(s) 46, 972
PpsI GAGTC 2 cut(s) 46, 972
Ppu21I YACGTR 1 cut(s) 704
Psp124BI GAGCTC 1 cut(s) 834
Psp6I CCWGG 3 cut(s) 405, 612, 820
PspEI GGTNACC 1 cut(s) 431
PspGI CCWGG 3 cut(s) 405, 612, 820
PspN4I GGNNCC 1 cut(s) 826
PspPI GGNCC 2 cut(s) 392, 818
PstI CTGCAG 1 cut(s) 565
PstNI CAGNNNCTG 1 cut(s) 626
RsaI GTAC 4 cut(s) 156, 304, 316, 706
RsaNI GTAC 4 cut(s) 155, 303, 315, 705
RseI CAYNNNNRTG 1 cut(s) 636
SacI GAGCTC 1 cut(s) 834
SalI GTCGAC 2 cut(s) 536, 684
SatI GCNGC 4 cut(s) 14, 499, 842, 926
Sau3AI GATC 7 cut(s) 28, 41, 160, 183, 522, 868, 883
Sau96I GGNCC 2 cut(s) 392, 818
SchI GAGTC 2 cut(s) 46, 973
ScrFI CCNGG 3 cut(s) 407, 614, 822
SduI GDGCHC 2 cut(s) 581, 834
SexAI ACCWGGT 1 cut(s) 820
SfaNI GCATC 1 cut(s) 443
SfcI CTRYAG 2 cut(s) 553, 561
SinI GGWCC 2 cut(s) 392, 818
SmiMI CAYNNNNRTG 1 cut(s) 636
Sse9I AATT 4 cut(s) 121, 241, 694, 956
SsiI CCGC 2 cut(s) 499, 841
SspI AATATT 1 cut(s) 586
SspMI CTAG 6 cut(s) 32, 39, 105, 422, 728, 981
SstI GAGCTC 1 cut(s) 834
StyD4I CCNGG 3 cut(s) 405, 612, 820
StyI CCWWGG 2 cut(s) 727, 747
TaaI ACNGT 6 cut(s) 340, 535, 622, 635, 709, 776
TaiI ACGT 3 cut(s) 96, 273, 706
TaqI TCGA 4 cut(s) 366, 537, 685, 722
TaqII GACCGA 1 cut(s) 845
TasI AATT 4 cut(s) 121, 241, 694, 956
TauI GCSGC 2 cut(s) 501, 844
TscAI CASTG 5 cut(s) 417, 532, 538, 565, 638
TseFI GTSAC 2 cut(s) 431, 475
TseI GCWGC 2 cut(s) 13, 925
Tsp45I GTSAC 2 cut(s) 431, 475
TspDTI ATGAA 5 cut(s) 346, 532, 703, 796, 824
TspGWI ACGGA 1 cut(s) 927
TspRI CASTG 5 cut(s) 417, 532, 538, 565, 638
Van91I CCANNNNNTGG 2 cut(s) 412, 753
VpaK11BI GGWCC 2 cut(s) 392, 818
XapI RAATTY 1 cut(s) 121
XbaI TCTAGA 1 cut(s) 31
XmaJI CCTAGG 1 cut(s) 727
XmiI GTMKAC 2 cut(s) 537, 685
XspI CTAG 6 cut(s) 32, 39, 105, 422, 728, 981
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.