RLG00000024178

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0001935
Physical Location & Seq
Reverse (-)
32788422 .. 32792233
3812 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024178

Sequence Viewer

Length: 2274 bp
ATGGTTATGGGGGAGGGGAGAGGCGGGTTGGTGCCGTATATGGTTCCTATTTCTCGGTGGTGGAAGCTGGATCCTACTTGGCTGTTGTTGGATCAGAGGACTGTAGTGTTGGTGGGGTGGCGTTGCCGGCCTCCCCTTCTTAATTCTTCTGTTGGTGGGGTCGATGGTGCTGCACGGGGGCTATGCTTCTGGCCCTCTGACGGGGGTGGAGCTTCAGTGGCTGTGGCGGTGCGGTGTTTGATTTTTGATCTCGTGCAGCCAAGTACTGGGTGTTTTTCCTTGCTTGTCGGTTATGGGGATGGAGGTTTCCAGGGCGACAATGTTCCTACTTGGGCTCTCTGGGCTGGTGATCGATCTGAGGATGAGGATGCTCAACGTGGTGGCTATTTAGGCTATGCTGGTGATGGCCTGAAGGAGTTCGGTAGGGGCGACGACGTCTTTTCCGGCAACAGCGACGGCGACATGCAACTTGTAATTGATAACTCTCCAAAATTTGACCATGAGGAGCGGTCCTCTCCCACTTTTGCATATATATGCATACAAAGGAATGATCGAGTCCATTGCAGTATTGAATTTGCTCTGCAACTTTTCACATCTCATCATTTACATTTCAGTACAACCCTCACGATTCCATTGGAGAAGATGAAGTTCTCCTATCCTTCCATTATTTTATTTATCCATTTCTCTGCTCTGTTCACTAACCCACATGCTAGCCCTAGTTATACTATTGGCTCCTCTCGCATCAGAAAAGTTTCATTGCCTTTGTTCAAAGACTCATTGGTGTCGACCATGGAACTTGTACAAAATGTGACCTCTACCATGTCCCAAATCCATGATGATGATGGTGAGCCATATCTCAATGTTACTATTCAACATTGTGCAAATTTCCTTGGCGAAATTGCTGGAGTCTTGAATTGGATCCTTTCTTCGATTAATCAGGAATACACTAAAGCTTCATCTGGAGGTTATAATCAATCTTATACAACAGGTGATGTTATTTCCCATGCGAGGACGTTGTTGAATGCTACACATCATGGCAGCCAAGAAACATGTATGGAAGTGCTCAATGGTACCAAACACTCCATGGTTGCAGGGAACCTCAAACAAGTGACCGCATCTATCTATGAAATCTTTGATATGTTGCAGATGCTACAAGGTAGTCCTGATTTTTCCAAGGCTACTGGTGCTATACCAATTGGTTCGACTGGACTTGATGACTTCAAGATTACGCTAAAACCAAACGTCACTGTTTCACAAGATGGGAAGGGAAATTTCAAGCTAATAATGGATGCGATCGCGGCTGCACCAAGTCACTCGACCAAGCACTTTGTGATACTTGTTAAGAAGGGGGTTTACAACGAATATGTGAACATCAACAACACAAAATCGAATATGATCCTGATTGGAGAGGGTAAGGATGCTACTATCATTTCCGGGAGTAAAAGCGCAGGCAGCGGTTGGACAACATTTAATACAGCCACATTTGCTGTTAGAGCCCAAGGATTCGTTGCAATTGACATAGGGTTCGAGAACACAGCAGGTCAAAAAAATACGCAAGCGGTCGCACTTCTGTCCGGCGGCGACCACTCTGTCTTCTATCGCTGCAAGATAACAGGAAATCAAGACACATTGCTTGTGCAAGCCGGCCGCCAGTTCTTCCGAGAATGCCAAATCACCGGCACTGTTGACTTCATCTTTGGCTATGGCACTGTCGTCTTCCAAAAATGCAACATTTACATCAAGAAAAACCAAATTGGCGGCACTAGTGTCATCGCTGCCCACGGCCGCAACTCCTCAACAGACTCATCCGGCTTTTCATTCCAATTCTGCAATATCGATGGAGACCCTAAGGTGTCCACCGTGAAGCCAACTCAAGAAGGCGCGTACCTCGGTCGGAGTTGGGGCATTTATGCACGAACAGTGTTCATGCAGTCGTCTATAAGTGATGTTCTAAGGCCGGAGGGTTGGCTTCAACGGCATGTAGATCCTGTAGACAAACTATATTTCGGTGAGTACCAGAATTCTGGACCTGGTGCGGCGCTTGGAGGTCGGGTGAAGTGGCCTGGTTTTCATAAGATGAGCGCATCTGATGCTGATAAATTTACGGTGGCCAACTTTATTGATGGGAATTCATGGCTAGCTTCGTTAGGTATCCCATACACACAGGGATTAGATCGGTCGGCCGATCCGCCAGCCAATCCGCCAATGGCAGCTTCAACAATCACAGTCACAACCTACAAAAAGCTCATTTCGAGTTTTTACACCAGCGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
PMEI Pectinesterase

Protein Analysis

758

Amino Acids

82.29

Weight (kDa)

6.26

Isoelectric Point (pI)

29.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 414 - 710 9.5e-112 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 969
AasI GACNNNNNNGTC 1 cut(s) 1589
AatII GACGTC 1 cut(s) 438
Acc36I ACCTGC 1 cut(s) 1529
Acc65I GGTACC 1 cut(s) 1070
AccB1I GGYRCC 2 cut(s) 31, 1070
AccB7I CCANNNNNTGG 1 cut(s) 266
AccBSI CCGCTC 1 cut(s) 508
AccI GTMKAC 2 cut(s) 785, 1992
AccII CGCG 2 cut(s) 1298, 1883
AclWI GGATC 8 cut(s) 65, 78, 99, 913, 926, 1390, 1979, 2180
AcoI YGGCCR 4 cut(s) 1645, 1783, 2109, 2181
AcsI RAATTY 7 cut(s) 491, 572, 883, 1270, 2020, 2099, 2128
AcuI CTGAAG 2 cut(s) 198, 431
AcyI GRCGYC 1 cut(s) 435
AdeI CACNNNGTG 1 cut(s) 1330
AfaI GTAC 6 cut(s) 265, 616, 801, 1072, 1886, 2015
AfiI CCNNNNNNNGG 4 cut(s) 200, 201, 266, 1008
AflIII ACRYGT 1 cut(s) 1049
AgsI TTSAA 9 cut(s) 572, 769, 872, 913, 1021, 1222, 1276, 1973, 2217
AhlI ACTAGT 1 cut(s) 1763
AjnI CCWGG 3 cut(s) 309, 2029, 2062
AjuI GAANNNNNNNTTGG 2 cut(s) 2197, 2229
AluBI AGCT 7 cut(s) 67, 212, 953, 1279, 2141, 2213, 2245
AluI AGCT 7 cut(s) 67, 212, 953, 1279, 2141, 2213, 2245
Alw21I GWGCWC 1 cut(s) 1065
Alw26I GTCTC 1 cut(s) 1836
AlwI GGATC 8 cut(s) 65, 78, 99, 913, 926, 1390, 1979, 2180
AlwNI CAGNNNCTG 1 cut(s) 221
AoxI GGCC 9 cut(s) 128, 191, 406, 1645, 1783, 1955, 2060, 2109, 2181
ApeKI GCWGC 8 cut(s) 170, 256, 1038, 1301, 1452, 1602, 1775, 2210
ApoI RAATTY 7 cut(s) 491, 572, 883, 1270, 2020, 2099, 2128
ArsI GACNNNNNNTTYG 4 cut(s) 1508, 1540, 1679, 1711
AseI ATTAAT 1 cut(s) 933
AsiSI GCGATCGC 1 cut(s) 1296
Asp700I GAANNNNTTC 2 cut(s) 416, 751
Asp718I GGTACC 1 cut(s) 1070
AspLEI GCGC 4 cut(s) 1448, 1883, 2041, 2084
AspS9I GGNCC 3 cut(s) 192, 510, 2027
AsuC2I CCSGG 1 cut(s) 1435
AsuHPI GGTGA 7 cut(s) 359, 413, 857, 1001, 1666, 2021, 2065
AsuNHI GCTAGC 2 cut(s) 710, 2137
AvaII GGWCC 2 cut(s) 510, 2027
AxyI CCTNAGG 1 cut(s) 1848
BalI TGGCCA 1 cut(s) 2111
BamHI GGATCC 2 cut(s) 70, 918
BanI GGYRCC 2 cut(s) 31, 1070
BanII GRGCYC 2 cut(s) 337, 1498
BarI GAAGNNNNNNTAC 2 cut(s) 1337, 1369
BauI CACGAG 1 cut(s) 251
BbsI GAAGAC 2 cut(s) 1585, 1708
Bbv12I GWGCWC 1 cut(s) 1065
BbvI GCAGC 8 cut(s) 157, 268, 1050, 1288, 1464, 1589, 1762, 2222
BccI CCATC 7 cut(s) 158, 293, 398, 836, 1253, 1832, 2117
BceAI ACGGC 4 cut(s) 19, 472, 1798, 1991
BcgI CGANNNNNNTGC 4 cut(s) 152, 186, 543, 577
BciT130I CCWGG 3 cut(s) 311, 2031, 2064
BciVI GTATCC 1 cut(s) 2162
BcnI CCSGG 1 cut(s) 1435
BcoDI GTCTC 1 cut(s) 1836
BcuI ACTAGT 1 cut(s) 1763
BfaI CTAG 4 cut(s) 711, 717, 1764, 2138
BfmI CTRYAG 2 cut(s) 102, 1989
BfoI RGCGCY 1 cut(s) 2042
BfuAI ACCTGC 1 cut(s) 1529
BfuI GTATCC 1 cut(s) 2162
BmcAI AGTACT 1 cut(s) 265
Bme1390I CCNGG 4 cut(s) 311, 1435, 2031, 2064
Bme18I GGWCC 2 cut(s) 510, 2027
BmgT120I GGNCC 3 cut(s) 192, 510, 2027
BmiI GGNNCC 7 cut(s) 33, 45, 72, 733, 920, 1072, 1097
BmrFI CCNGG 4 cut(s) 311, 1435, 2031, 2064
BmrI ACTGGG 1 cut(s) 276
BmsI GCATC 8 cut(s) 358, 750, 1124, 1137, 1279, 1408, 2080, 2093
BmtI GCTAGC 2 cut(s) 714, 2141
BmuI ACTGGG 1 cut(s) 276
BpiI GAAGAC 2 cut(s) 1585, 1708
BplI GAGNNNNNCTC 2 cut(s) 497, 529
BpmI CTGGAG 2 cut(s) 924, 981
BpuEI CTTGAG 1 cut(s) 1857
BpuMI CCSGG 1 cut(s) 1435
Bsa29I ATCGAT 2 cut(s) 352, 1836
BsaHI GRCGYC 1 cut(s) 435
BsaI GGTCTC 1 cut(s) 1836
BsaJI CCNNGG 8 cut(s) 310, 789, 889, 1083, 1173, 1498, 1780, 1888
BsaXI ACNNNNNCTCC 4 cut(s) 201, 231, 2037, 2067
Bsc4I CCNNNNNNNGG 4 cut(s) 200, 201, 266, 1008
Bse118I RCCGGY 3 cut(s) 126, 1643, 1676
Bse1I ACTGG 4 cut(s) 271, 1186, 1210, 1651
Bse21I CCTNAGG 1 cut(s) 1848
Bse3DI GCAATG 3 cut(s) 559, 755, 1628
BseBI CCWGG 3 cut(s) 311, 2031, 2064
BseCI ATCGAT 2 cut(s) 352, 1836
BseDI CCNNGG 8 cut(s) 310, 789, 889, 1083, 1173, 1498, 1780, 1888
BseGI GGATG 6 cut(s) 304, 367, 373, 1294, 1423, 1805
BseLI CCNNNNNNNGG 4 cut(s) 200, 201, 266, 1008
BseMI GCAATG 3 cut(s) 559, 755, 1628
BseMII CTCAG 1 cut(s) 348
BseNI ACTGG 4 cut(s) 271, 1186, 1210, 1651
BseRI GAGGAG 3 cut(s) 518, 724, 1783
BseX3I CGGCCG 3 cut(s) 1645, 1783, 2181
BseXI GCAGC 8 cut(s) 157, 268, 1050, 1288, 1464, 1589, 1762, 2222
BsgI GTGCAG 3 cut(s) 156, 275, 1287
Bsh1236I CGCG 2 cut(s) 1298, 1883
Bsh1285I CGRYCG 7 cut(s) 1296, 1563, 1648, 1786, 1894, 2180, 2184
BshFI GGCC 9 cut(s) 130, 193, 408, 1647, 1785, 1957, 2062, 2111, 2183
BshNI GGYRCC 2 cut(s) 31, 1070
BshVI ATCGAT 2 cut(s) 352, 1836
BsiEI CGRYCG 7 cut(s) 1296, 1563, 1648, 1786, 1894, 2180, 2184
BsiHKAI GWGCWC 1 cut(s) 1065
BsiSI CCGG 8 cut(s) 127, 444, 1434, 1575, 1644, 1677, 1809, 1958
BslFI GGGAC 1 cut(s) 808
BslI CCNNNNNNNGG 4 cut(s) 200, 201, 266, 1008
BsmAI GTCTC 1 cut(s) 1836
BsmFI GGGAC 1 cut(s) 808
BsmI GAATGC 2 cut(s) 1027, 1670
BsnI GGCC 9 cut(s) 130, 193, 408, 1647, 1785, 1957, 2062, 2111, 2183
Bso31I GGTCTC 1 cut(s) 1836
Bsp1286I GDGCHC 3 cut(s) 337, 1065, 1498
Bsp1407I TGTACA 1 cut(s) 799
Bsp19I CCATGG 2 cut(s) 789, 1083
BspANI GGCC 9 cut(s) 130, 193, 408, 1647, 1785, 1957, 2062, 2111, 2183
BspCNI CTCAG 1 cut(s) 349
BspDI ATCGAT 2 cut(s) 352, 1836
BspFNI CGCG 2 cut(s) 1298, 1883
BspLI GGNNCC 7 cut(s) 33, 45, 72, 733, 920, 1072, 1097
BspMI ACCTGC 1 cut(s) 1529
BspOI GCTAGC 2 cut(s) 714, 2141
BspPI GGATC 8 cut(s) 65, 78, 99, 913, 926, 1390, 1979, 2180
BspT107I GGYRCC 2 cut(s) 31, 1070
BspTNI GGTCTC 1 cut(s) 1836
BsrBI CCGCTC 1 cut(s) 508
BsrDI GCAATG 3 cut(s) 559, 755, 1628
BsrFI RCCGGY 3 cut(s) 126, 1643, 1676
BsrGI TGTACA 1 cut(s) 799
BsrI ACTGG 4 cut(s) 271, 1186, 1210, 1651
BssAI RCCGGY 3 cut(s) 126, 1643, 1676
BssECI CCNNGG 8 cut(s) 310, 789, 889, 1083, 1173, 1498, 1780, 1888
BssNI GRCGYC 1 cut(s) 435
BssSI CACGAG 1 cut(s) 251
BssT1I CCWWGG 5 cut(s) 789, 889, 1083, 1173, 1498
Bst2BI CACGAG 1 cut(s) 251
Bst2UI CCWGG 3 cut(s) 311, 2031, 2064
Bst4CI ACNGT 8 cut(s) 103, 1249, 1684, 1711, 1861, 1921, 2107, 2227
BstACI GRCGYC 1 cut(s) 435
BstAPI GCANNNNNTGC 1 cut(s) 2090
BstAUI TGTACA 1 cut(s) 799
BstC8I GCNNGC 8 cut(s) 128, 712, 1450, 1557, 1641, 1645, 2139, 2193
BstDEI CTNAG 3 cut(s) 357, 1848, 1952
BstDSI CCRYGG 3 cut(s) 789, 1083, 1780
BstF5I GGATG 6 cut(s) 304, 367, 373, 1294, 1423, 1805
BstFNI CGCG 2 cut(s) 1298, 1883
BstH2I RGCGCY 1 cut(s) 2042
BstHHI GCGC 4 cut(s) 1448, 1883, 2041, 2084
BstMAI GTCTC 1 cut(s) 1836
BstMCI CGRYCG 7 cut(s) 1296, 1563, 1648, 1786, 1894, 2180, 2184
BstNI CCWGG 3 cut(s) 311, 2031, 2064
BstNSI RCATGY 4 cut(s) 466, 710, 1053, 1982
BstSCI CCNGG 4 cut(s) 309, 1433, 2029, 2062
BstSFI CTRYAG 2 cut(s) 102, 1989
BstUI CGCG 2 cut(s) 1298, 1883
BstV1I GCAGC 8 cut(s) 157, 268, 1050, 1288, 1464, 1589, 1762, 2222
BstV2I GAAGAC 2 cut(s) 1585, 1708
BstX2I RGATCY 3 cut(s) 70, 918, 1984
BstXI CCANNNNNNTGG 1 cut(s) 2024
BstYI RGATCY 3 cut(s) 70, 918, 1984
BstZI CGGCCG 3 cut(s) 1645, 1783, 2181
Bsu15I ATCGAT 2 cut(s) 352, 1836
Bsu36I CCTNAGG 1 cut(s) 1848
BsuI GTATCC 1 cut(s) 2162
BsuRI GGCC 9 cut(s) 130, 193, 408, 1647, 1785, 1957, 2062, 2111, 2183
BsuTUI ATCGAT 2 cut(s) 352, 1836
BtgI CCRYGG 3 cut(s) 789, 1083, 1780
BtgZI GCGATG 1 cut(s) 1756
BtsCI GGATG 6 cut(s) 304, 367, 373, 1294, 1423, 1805
BtsIMutI CAGTG 5 cut(s) 222, 1245, 1680, 1707, 1926
BveI ACCTGC 1 cut(s) 1529
Cac8I GCNNGC 8 cut(s) 128, 712, 1450, 1557, 1641, 1645, 2139, 2193
CaiI CAGNNNCTG 1 cut(s) 221
CfoI GCGC 4 cut(s) 1448, 1883, 2041, 2084
Cfr10I RCCGGY 3 cut(s) 126, 1643, 1676
Cfr13I GGNCC 3 cut(s) 192, 510, 2027
ClaI ATCGAT 2 cut(s) 352, 1836
CsiI ACCWGGT 1 cut(s) 2029
Csp6I GTAC 6 cut(s) 264, 615, 800, 1071, 1885, 2014
CviQI GTAC 6 cut(s) 264, 615, 800, 1071, 1885, 2014
DdeI CTNAG 3 cut(s) 357, 1848, 1952
DraIII CACNNNGTG 1 cut(s) 1330
DrdI GACNNNNNNGTC 1 cut(s) 1589
DseDI GACNNNNNNGTC 1 cut(s) 1589
EaeI YGGCCR 4 cut(s) 1645, 1783, 2109, 2181
EagI CGGCCG 3 cut(s) 1645, 1783, 2181
EciI GGCGGA 2 cut(s) 2178, 2190
EclXI CGGCCG 3 cut(s) 1645, 1783, 2181
Eco130I CCWWGG 5 cut(s) 789, 889, 1083, 1173, 1498
Eco24I GRGCYC 2 cut(s) 337, 1498
Eco31I GGTCTC 1 cut(s) 1836
Eco47I GGWCC 2 cut(s) 510, 2027
Eco52I CGGCCG 3 cut(s) 1645, 1783, 2181
Eco57I CTGAAG 2 cut(s) 198, 431
Eco81I CCTNAGG 1 cut(s) 1848
EcoRI GAATTC 2 cut(s) 2020, 2128
EcoRII CCWGG 3 cut(s) 309, 2029, 2062
EcoT14I CCWWGG 5 cut(s) 789, 889, 1083, 1173, 1498
EcoT22I ATGCAT 1 cut(s) 539
EcoT38I GRGCYC 2 cut(s) 337, 1498
ErhI CCWWGG 5 cut(s) 789, 889, 1083, 1173, 1498
FaqI GGGAC 1 cut(s) 808
FauI CCCGC 1 cut(s) 17
FblI GTMKAC 2 cut(s) 785, 1992
FokI GGATG 6 cut(s) 311, 374, 380, 1301, 1430, 1792
FriOI GRGCYC 2 cut(s) 337, 1498
FspBI CTAG 4 cut(s) 711, 717, 1764, 2138
GlaI GCGC 4 cut(s) 1447, 1882, 2040, 2083
GsuI CTGGAG 2 cut(s) 924, 981
HaeII RGCGCY 1 cut(s) 2042
HaeIII GGCC 9 cut(s) 130, 193, 408, 1647, 1785, 1957, 2062, 2111, 2183
HapII CCGG 8 cut(s) 127, 444, 1434, 1575, 1644, 1677, 1809, 1958
HhaI GCGC 4 cut(s) 1448, 1883, 2041, 2084
Hin1I GRCGYC 1 cut(s) 435
Hin6I GCGC 4 cut(s) 1446, 1881, 2039, 2082
HinP1I GCGC 4 cut(s) 1446, 1881, 2039, 2082
HincII GTYRAC 2 cut(s) 786, 1687
HindII GTYRAC 2 cut(s) 786, 1687
HindIII AAGCTT 1 cut(s) 951
HinfI GANTC 6 cut(s) 555, 628, 773, 906, 1503, 1802
HpaII CCGG 8 cut(s) 127, 444, 1434, 1575, 1644, 1677, 1809, 1958
HphI GGTGA 7 cut(s) 359, 413, 857, 1001, 1666, 2021, 2065
Hpy166II GTNNAC 7 cut(s) 696, 786, 1354, 1369, 1687, 1857, 1993
Hpy188I TCNGA 7 cut(s) 96, 199, 358, 746, 1661, 1896, 2089
Hpy8I GTNNAC 7 cut(s) 696, 786, 1354, 1369, 1687, 1857, 1993
Hpy99I CGWCG 3 cut(s) 434, 437, 458
HpyAV CCTTC 6 cut(s) 146, 406, 669, 1258, 1339, 1871
HpyCH4III ACNGT 8 cut(s) 103, 1249, 1684, 1711, 1861, 1921, 2107, 2227
HpyCH4IV ACGT 4 cut(s) 376, 435, 1013, 1242
HpyF3I CTNAG 3 cut(s) 357, 1848, 1952
HpySE526I ACGT 4 cut(s) 376, 435, 1013, 1242
Hsp92I GRCGYC 1 cut(s) 435
HspAI GCGC 4 cut(s) 1446, 1881, 2039, 2082
KpnI GGTACC 1 cut(s) 1074
KroI GCCGGC 2 cut(s) 126, 1643
KroNI GCCGGC 2 cut(s) 128, 1645
LmnI GCTCC 3 cut(s) 209, 505, 737
Lsp1109I GCAGC 8 cut(s) 157, 268, 1050, 1288, 1464, 1589, 1762, 2222
LweI GCATC 8 cut(s) 358, 750, 1124, 1137, 1279, 1408, 2080, 2093
MabI ACCWGGT 1 cut(s) 2029
MaeI CTAG 4 cut(s) 711, 717, 1764, 2138
MaeII ACGT 4 cut(s) 376, 435, 1013, 1242
MaeIII GTNAC 6 cut(s) 808, 862, 1108, 1243, 1310, 2227
MbiI CCGCTC 1 cut(s) 508
MboII GAAGA 6 cut(s) 138, 652, 918, 1585, 1648, 1708
MfeI CAATTG 2 cut(s) 1194, 1512
MflI RGATCY 3 cut(s) 70, 918, 1984
MhlI GDGCHC 3 cut(s) 337, 1065, 1498
MlsI TGGCCA 1 cut(s) 2111
MluNI TGGCCA 1 cut(s) 2111
MlyI GAGTC 4 cut(s) 564, 767, 915, 1796
MmeI TCCRAC 3 cut(s) 69, 1439, 1874
Mox20I TGGCCA 1 cut(s) 2111
Mph1103I ATGCAT 1 cut(s) 539
MroNI GCCGGC 2 cut(s) 126, 1643
MroXI GAANNNNTTC 2 cut(s) 416, 751
MscI TGGCCA 1 cut(s) 2111
MseI TTAA 4 cut(s) 141, 933, 1341, 1470
MslI CAYNNNNRTG 2 cut(s) 532, 837
Msp20I TGGCCA 1 cut(s) 2111
MspA1I CMGCKG 1 cut(s) 1455
MspI CCGG 8 cut(s) 127, 444, 1434, 1575, 1644, 1677, 1809, 1958
MspR9I CCNGG 4 cut(s) 311, 1435, 2031, 2064
MunI CAATTG 2 cut(s) 1194, 1512
Mva1269I GAATGC 2 cut(s) 1027, 1670
MvaI CCWGG 3 cut(s) 311, 2031, 2064
MvnI CGCG 2 cut(s) 1298, 1883
NaeI GCCGGC 2 cut(s) 128, 1645
NciI CCSGG 1 cut(s) 1435
NcoI CCATGG 2 cut(s) 789, 1083
NgoMIV GCCGGC 2 cut(s) 126, 1643
NheI GCTAGC 2 cut(s) 710, 2137
NlaIV GGNNCC 7 cut(s) 33, 45, 72, 733, 920, 1072, 1097
NmuCI GTSAC 5 cut(s) 808, 1108, 1243, 1310, 2227
NsiI ATGCAT 1 cut(s) 539
NspI RCATGY 4 cut(s) 466, 710, 1053, 1982
PciI ACATGT 1 cut(s) 1049
PcsI WCGNNNNNNNCGW 1 cut(s) 426
PctI GAATGC 2 cut(s) 1027, 1670
PdiI GCCGGC 2 cut(s) 128, 1645
PdmI GAANNNNTTC 2 cut(s) 416, 751
PfeI GAWTC 2 cut(s) 628, 1503
PflFI GACNNNGTC 1 cut(s) 434
PflMI CCANNNNNTGG 1 cut(s) 266
PfoI TCCNGGA 1 cut(s) 1433
Ple19I CGATCG 1 cut(s) 1296
PleI GAGTC 4 cut(s) 563, 767, 914, 1796
PpsI GAGTC 4 cut(s) 563, 767, 914, 1796
PscI ACATGT 1 cut(s) 1049
PshBI ATTAAT 1 cut(s) 933
PsiI TTATAA 1 cut(s) 969
Psp6I CCWGG 3 cut(s) 309, 2029, 2062
PspGI CCWGG 3 cut(s) 309, 2029, 2062
PspN4I GGNNCC 7 cut(s) 33, 45, 72, 733, 920, 1072, 1097
PspPI GGNCC 3 cut(s) 192, 510, 2027
PstNI CAGNNNCTG 1 cut(s) 221
PsuI RGATCY 3 cut(s) 70, 918, 1984
PsyI GACNNNGTC 1 cut(s) 434
PvuI CGATCG 1 cut(s) 1296
RgaI GCGATCGC 1 cut(s) 1296
RsaI GTAC 6 cut(s) 265, 616, 801, 1072, 1886, 2015
RsaNI GTAC 6 cut(s) 264, 615, 800, 1071, 1885, 2014
RseI CAYNNNNRTG 2 cut(s) 532, 837
SalI GTCGAC 1 cut(s) 784
SaqAI TTAA 4 cut(s) 141, 933, 1341, 1470
Sau96I GGNCC 3 cut(s) 192, 510, 2027
ScaI AGTACT 1 cut(s) 265
SchI GAGTC 4 cut(s) 564, 767, 915, 1796
ScrFI CCNGG 4 cut(s) 311, 1435, 2031, 2064
SduI GDGCHC 3 cut(s) 337, 1065, 1498
SexAI ACCWGGT 1 cut(s) 2029
SfaAI GCGATCGC 1 cut(s) 1296
SfaNI GCATC 8 cut(s) 358, 750, 1124, 1137, 1279, 1408, 2080, 2093
SfcI CTRYAG 2 cut(s) 102, 1989
SgfI GCGATCGC 1 cut(s) 1296
SinI GGWCC 2 cut(s) 510, 2027
SmiMI CAYNNNNRTG 2 cut(s) 532, 837
SmlI CTYRAG 1 cut(s) 1872
SmoI CTYRAG 1 cut(s) 1872
SpeI ACTAGT 1 cut(s) 1763
SspMI CTAG 4 cut(s) 711, 717, 1764, 2138
StyD4I CCNGG 4 cut(s) 309, 1433, 2029, 2062
StyI CCWWGG 5 cut(s) 789, 889, 1083, 1173, 1498
TaaI ACNGT 8 cut(s) 103, 1249, 1684, 1711, 1861, 1921, 2107, 2227
TaiI ACGT 4 cut(s) 379, 438, 1016, 1245
TaqII GACCGA 2 cut(s) 1880, 2166
TatI WGTACW 3 cut(s) 263, 614, 799
TauI GCSGC 6 cut(s) 1301, 1581, 1650, 1761, 1788, 2039
TfiI GAWTC 2 cut(s) 628, 1503
Tru1I TTAA 4 cut(s) 141, 933, 1341, 1470
Tru9I TTAA 4 cut(s) 141, 933, 1341, 1470
TscAI CASTG 5 cut(s) 222, 1252, 1687, 1714, 1926
TseFI GTSAC 5 cut(s) 808, 1108, 1243, 1310, 2227
TseI GCWGC 8 cut(s) 170, 256, 1038, 1301, 1452, 1602, 1775, 2210
Tsp45I GTSAC 5 cut(s) 808, 1108, 1243, 1310, 2227
TspDTI ATGAA 9 cut(s) 659, 744, 945, 1140, 1681, 1806, 1915, 2060, 2121
TspRI CASTG 5 cut(s) 222, 1252, 1687, 1714, 1926
Tth111I GACNNNGTC 1 cut(s) 434
Van91I CCANNNNNTGG 1 cut(s) 266
VpaK11BI GGWCC 2 cut(s) 510, 2027
VspI ATTAAT 1 cut(s) 933
XapI RAATTY 7 cut(s) 491, 572, 883, 1270, 2020, 2099, 2128
XceI RCATGY 4 cut(s) 466, 710, 1053, 1982
XcmI CCANNNNNNNNNTGG 3 cut(s) 839, 1081, 2203
XmiI GTMKAC 2 cut(s) 785, 1992
XmnI GAANNNNTTC 2 cut(s) 416, 751
XspI CTAG 4 cut(s) 711, 717, 1764, 2138
ZraI GACGTC 1 cut(s) 436
ZrmI AGTACT 1 cut(s) 265
Zsp2I ATGCAT 1 cut(s) 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.