FvH4_6g17430

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001935
Physical Location & Seq
Forward (+)
11360126 .. 11361662
1537 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g17430.t1

Sequence Viewer

Length: 1272 bp
ATGAAGTTCTTCTATCATTCCATTCTTTTGTTCATTTATTTTTCTCCTCTATTCATTAACCCACATGCGAGGTCGACGTTGTTGAATGCTAGTCTTGTAAGCCAAGAAACATGTAAGGAAGTGCTCGATAGTACCAGAAGGAGCCTCGAACAAGTTACTACATCTATCTATCAGATCCTTGATACATTGCAAGTGCCACAGGGTACTCCTGATTTTTGCAAGGCCTCTGGTGCTCCTATACTAATTGGTTCGACTGGACCTGACGACGTAAAGATTCCGAAAACACCAAACGTCACTGTTTCACAAGATGGGAAGGGAAATTTCAAGCTGATAATGGATGCGATCAAAGCTGCACCGAGTCACTCAAATAACCACTATGTGATACTTGTCAAGAAAGGGGTTTACAAGGAATATGTGAACATCGACCAAACAAAAACGAATATAGTCTTGATGGGAGAGGGTAAAGAAGCCACTATCATTTCCGGGAGTAAAAGCGTAGGCGGCGGTCAGCAAACATTTGATTCAGCCACATTTGCTGTTAGTGCTCAAGGATTCGTTGCAATTGACATAGGGTTTGAGAACACAGCAGGTCAGAAAAATATGCAGGCAGTGGCGGTTCTATCCGCCGGCGACCACGCTGCCTTCCACCGGTGCAAGATAACAGGAAACCAGGACACATTGCTTGTGCATGCTGGCCGCCAGTTCTTCCGAGAATGCGAAATCATCTGCACCGTCGACTTCATCTTTGGCTATGGCACTGCTGTCTTCCAAAAATGCAACATTTACATCAGGAAAAACAAGATTGGTGGCACTAGCGTTATCTCCGCACACGGCCGTAACTCCTCAACAGAAGCATCAGGCTTTTCGTTCCACGATTGCGCCATCAATGGTGACCCCCAGCCCCCTACAAACTCAAGTACTCATGCAGGCGCGTACCTCGGTCGAAACTGGGGCACTTTTGCACGAACAGTGTTCATGCATTCAACTATAAGCAATGTTGTAAGGCCGGAGGGTTGGCTTCAGTGGCAGAGTGATCCTGTAGACAAACTATATTTCGGTGAGTTCCAAAATTCCGGCCCAGGTGCGGCGGATGGAGGTCGAGTCAAGTGGCCGGGGTTTCATCAGATGACCGCAGCTGATGCTGATAAATTTACAGTGGCCAAGTTTATTGATGGGAATTCATGGCTACCTGCGTTAGGTATCCCATTTTCACCAGGAGTGAAGCCATCAGCGGATCCGCCCAAATGGGGGCTTGAATATTCATCGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
PMEI Pectinesterase

Protein Analysis

424

Amino Acids

45.84

Weight (kDa)

8.08

Isoelectric Point (pI)

32.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 97 - 393 1e-111 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 578, 1198
AccI GTMKAC 3 cut(s) 74, 735, 1041
AccII CGCG 1 cut(s) 932
AclWI GGATC 4 cut(s) 169, 1028, 1229, 1242
AcoI YGGCCR 4 cut(s) 694, 832, 1109, 1158
AcsI RAATTY 4 cut(s) 319, 1069, 1148, 1177
AcuI CTGAAG 1 cut(s) 1004
AdeI CACNNNGTG 1 cut(s) 379
AfaI GTAC 4 cut(s) 133, 205, 919, 935
AfiI CCNNNNNNNGG 5 cut(s) 648, 1084, 1196, 1247, 1248
AflIII ACRYGT 1 cut(s) 110
AgeI ACCGGT 1 cut(s) 648
AgsI TTSAA 4 cut(s) 85, 325, 984, 1256
AjnI CCWGG 3 cut(s) 669, 1078, 1213
AluBI AGCT 3 cut(s) 328, 350, 1136
AluI AGCT 3 cut(s) 328, 350, 1136
Alw21I GWGCWC 3 cut(s) 126, 235, 547
AlwI GGATC 4 cut(s) 169, 1028, 1229, 1242
AoxI GGCC 7 cut(s) 222, 694, 832, 1004, 1075, 1109, 1158
ApeKI GCWGC 3 cut(s) 350, 638, 1133
ApoI RAATTY 4 cut(s) 319, 1069, 1148, 1177
ArsI GACNNNNNNTTYG 4 cut(s) 557, 589, 728, 760
AsiGI ACCGGT 1 cut(s) 648
Asp700I GAANNNNTTC 1 cut(s) 8
AspLEI GCGC 2 cut(s) 881, 932
AspS9I GGNCC 2 cut(s) 257, 1076
AsuC2I CCSGG 2 cut(s) 484, 1113
AsuHPI GGTGA 3 cut(s) 902, 1070, 1203
AvaII GGWCC 1 cut(s) 257
BaeGI GKGCMC 1 cut(s) 956
BalI TGGCCA 1 cut(s) 1160
BamHI GGATCC 1 cut(s) 1234
BbsI GAAGAC 1 cut(s) 757
Bbv12I GWGCWC 3 cut(s) 126, 235, 547
BbvI GCAGC 3 cut(s) 337, 625, 1145
BccI CCATC 6 cut(s) 302, 445, 890, 1085, 1166, 1234
BceAI ACGGC 2 cut(s) 819, 847
BcgI CGANNNNNNTGC 2 cut(s) 620, 654
BciT130I CCWGG 3 cut(s) 671, 1080, 1215
BciVI GTATCC 1 cut(s) 1211
BcnI CCSGG 2 cut(s) 484, 1113
BfaI CTAG 2 cut(s) 90, 813
BfmI CTRYAG 1 cut(s) 1038
BfuAI ACCTGC 2 cut(s) 578, 1198
BfuI GTATCC 1 cut(s) 1211
BisI GCNGC 6 cut(s) 351, 502, 639, 697, 1086, 1134
BlsI GCNGC 6 cut(s) 352, 503, 640, 698, 1087, 1135
BmcAI AGTACT 1 cut(s) 919
Bme1390I CCNGG 5 cut(s) 484, 671, 1080, 1113, 1215
Bme18I GGWCC 1 cut(s) 257
BmgT120I GGNCC 2 cut(s) 257, 1076
BmiI GGNNCC 2 cut(s) 143, 1236
BmrFI CCNGG 5 cut(s) 484, 671, 1080, 1113, 1215
BmrI ACTGGG 1 cut(s) 958
BmsI GCATC 3 cut(s) 328, 863, 1129
BmuI ACTGGG 1 cut(s) 958
BpiI GAAGAC 1 cut(s) 757
BpuEI CTTGAG 2 cut(s) 531, 898
BpuMI CCSGG 2 cut(s) 484, 1113
BsaJI CCNNGG 3 cut(s) 937, 1078, 1112
BsaWI WCCGGW 1 cut(s) 648
BsaXI ACNNNNNCTCC 2 cut(s) 1086, 1116
Bsc4I CCNNNNNNNGG 5 cut(s) 648, 1084, 1196, 1247, 1248
Bse118I RCCGGY 2 cut(s) 626, 648
Bse1I ACTGG 3 cut(s) 259, 700, 953
Bse3DI GCAATG 3 cut(s) 185, 677, 1000
BseBI CCWGG 3 cut(s) 671, 1080, 1215
BseDI CCNNGG 3 cut(s) 937, 1078, 1112
BseGI GGATG 2 cut(s) 343, 1096
BseLI CCNNNNNNNGG 5 cut(s) 648, 1084, 1196, 1247, 1248
BseMI GCAATG 3 cut(s) 185, 677, 1000
BseNI ACTGG 3 cut(s) 259, 700, 953
BseRI GAGGAG 2 cut(s) 36, 832
BseSI GKGCMC 1 cut(s) 956
BseX3I CGGCCG 1 cut(s) 832
BseXI GCAGC 3 cut(s) 337, 625, 1145
BseYI CCCAGC 1 cut(s) 897
BsgI GTGCAG 2 cut(s) 336, 712
Bsh1236I CGCG 1 cut(s) 932
Bsh1285I CGRYCG 2 cut(s) 835, 943
BshFI GGCC 7 cut(s) 224, 696, 834, 1006, 1077, 1111, 1160
BshTI ACCGGT 1 cut(s) 648
BsiEI CGRYCG 2 cut(s) 835, 943
BsiHKAI GWGCWC 3 cut(s) 126, 235, 547
BsiSI CCGG 6 cut(s) 483, 627, 649, 1007, 1074, 1112
BslI CCNNNNNNNGG 5 cut(s) 648, 1084, 1196, 1247, 1248
BsmI GAATGC 3 cut(s) 91, 719, 979
BsnI GGCC 7 cut(s) 224, 696, 834, 1006, 1077, 1111, 1160
Bsp1286I GDGCHC 4 cut(s) 126, 235, 547, 956
Bsp143I GATC 4 cut(s) 174, 342, 1033, 1234
BspANI GGCC 7 cut(s) 224, 696, 834, 1006, 1077, 1111, 1160
BspFNI CGCG 1 cut(s) 932
BspHI TCATGA 1 cut(s) 1268
BspLI GGNNCC 2 cut(s) 143, 1236
BspMI ACCTGC 2 cut(s) 578, 1198
BspPI GGATC 4 cut(s) 169, 1028, 1229, 1242
BsrDI GCAATG 3 cut(s) 185, 677, 1000
BsrFI RCCGGY 2 cut(s) 626, 648
BsrI ACTGG 3 cut(s) 259, 700, 953
BssAI RCCGGY 2 cut(s) 626, 648
BssECI CCNNGG 3 cut(s) 937, 1078, 1112
BssMI GATC 4 cut(s) 174, 342, 1033, 1234
Bst2UI CCWGG 3 cut(s) 671, 1080, 1215
Bst4CI ACNGT 4 cut(s) 298, 733, 970, 1156
BstAPI GCANNNNNTGC 1 cut(s) 1139
BstC8I GCNNGC 5 cut(s) 606, 628, 690, 694, 928
BstEII GGTNACC 1 cut(s) 890
BstENI CCTNNNNNAGG 1 cut(s) 1194
BstF5I GGATG 2 cut(s) 343, 1096
BstFNI CGCG 1 cut(s) 932
BstHHI GCGC 2 cut(s) 881, 932
BstKTI GATC 4 cut(s) 177, 345, 1036, 1237
BstMBI GATC 4 cut(s) 174, 342, 1033, 1234
BstMCI CGRYCG 2 cut(s) 835, 943
BstMWI GCNNNNNNNGC 7 cut(s) 230, 347, 501, 533, 542, 1024, 1139
BstNI CCWGG 3 cut(s) 671, 1080, 1215
BstNSI RCATGY 3 cut(s) 68, 114, 692
BstPI GGTNACC 1 cut(s) 890
BstSCI CCNGG 5 cut(s) 482, 669, 1078, 1111, 1213
BstSFI CTRYAG 1 cut(s) 1038
BstSLI GKGCMC 1 cut(s) 956
BstUI CGCG 1 cut(s) 932
BstV1I GCAGC 3 cut(s) 337, 625, 1145
BstV2I GAAGAC 1 cut(s) 757
BstX2I RGATCY 2 cut(s) 174, 1234
BstYI RGATCY 2 cut(s) 174, 1234
BstZI CGGCCG 1 cut(s) 832
BsuI GTATCC 1 cut(s) 1211
BsuRI GGCC 7 cut(s) 224, 696, 834, 1006, 1077, 1111, 1160
BtsCI GGATG 2 cut(s) 343, 1096
BtsI GCAGTG 2 cut(s) 615, 756
BtsIMutI CAGTG 6 cut(s) 294, 615, 756, 975, 1028, 1161
BveI ACCTGC 2 cut(s) 578, 1198
Cac8I GCNNGC 5 cut(s) 606, 628, 690, 694, 928
CciI TCATGA 1 cut(s) 1268
CfoI GCGC 2 cut(s) 881, 932
Cfr10I RCCGGY 2 cut(s) 626, 648
Cfr13I GGNCC 2 cut(s) 257, 1076
Csp6I GTAC 4 cut(s) 132, 204, 918, 934
CspAI ACCGGT 1 cut(s) 648
CspCI CAANNNNNGTGG 2 cut(s) 787, 822
CviAII CATG 7 cut(s) 65, 111, 689, 923, 976, 1182, 1269
CviQI GTAC 4 cut(s) 132, 204, 918, 934
DpnI GATC 4 cut(s) 176, 344, 1035, 1236
DpnII GATC 4 cut(s) 174, 342, 1033, 1234
DraIII CACNNNGTG 1 cut(s) 379
EaeI YGGCCR 4 cut(s) 694, 832, 1109, 1158
EagI CGGCCG 1 cut(s) 832
EciI GGCGGA 3 cut(s) 613, 1103, 1227
EclXI CGGCCG 1 cut(s) 832
Eco147I AGGCCT 1 cut(s) 224
Eco47I GGWCC 1 cut(s) 257
Eco52I CGGCCG 1 cut(s) 832
Eco57I CTGAAG 1 cut(s) 1004
Eco91I GGTNACC 1 cut(s) 890
EcoNI CCTNNNNNAGG 1 cut(s) 1194
EcoO65I GGTNACC 1 cut(s) 890
EcoRI GAATTC 1 cut(s) 1177
EcoRII CCWGG 3 cut(s) 669, 1078, 1213
EcoT22I ATGCAT 1 cut(s) 981
FaeI CATG 7 cut(s) 68, 114, 692, 926, 979, 1185, 1272
FatI CATG 7 cut(s) 64, 110, 688, 922, 975, 1181, 1268
FblI GTMKAC 3 cut(s) 74, 735, 1041
Fnu4HI GCNGC 6 cut(s) 351, 502, 639, 697, 1086, 1134
FokI GGATG 2 cut(s) 350, 1103
Fsp4HI GCNGC 6 cut(s) 351, 502, 639, 697, 1086, 1134
FspBI CTAG 2 cut(s) 90, 813
GlaI GCGC 2 cut(s) 880, 931
GluI GCNGC 6 cut(s) 351, 502, 639, 697, 1086, 1134
GsaI CCCAGC 1 cut(s) 901
HaeIII GGCC 7 cut(s) 224, 696, 834, 1006, 1077, 1111, 1160
HapII CCGG 6 cut(s) 483, 627, 649, 1007, 1074, 1112
HhaI GCGC 2 cut(s) 881, 932
Hin1II CATG 7 cut(s) 68, 114, 692, 926, 979, 1185, 1272
Hin6I GCGC 2 cut(s) 879, 930
HinP1I GCGC 2 cut(s) 879, 930
HincII GTYRAC 2 cut(s) 75, 736
HindII GTYRAC 2 cut(s) 75, 736
HinfI GANTC 5 cut(s) 274, 358, 521, 552, 1101
HpaII CCGG 6 cut(s) 483, 627, 649, 1007, 1074, 1112
HphI GGTGA 3 cut(s) 902, 1070, 1203
Hpy166II GTNNAC 5 cut(s) 75, 403, 418, 736, 1042
Hpy188I TCNGA 5 cut(s) 174, 279, 594, 710, 1125
Hpy188III TCNNGA 5 cut(s) 209, 391, 448, 790, 1269
Hpy8I GTNNAC 5 cut(s) 75, 403, 418, 736, 1042
Hpy99I CGWCG 3 cut(s) 79, 269, 737
HpyAV CCTTC 3 cut(s) 132, 307, 652
HpyCH4III ACNGT 4 cut(s) 298, 733, 970, 1156
HpyCH4IV ACGT 3 cut(s) 77, 267, 291
HpyF10VI GCNNNNNNNGC 7 cut(s) 230, 347, 501, 533, 542, 1024, 1139
HpySE526I ACGT 3 cut(s) 77, 267, 291
Hsp92II CATG 7 cut(s) 68, 114, 692, 926, 979, 1185, 1272
HspAI GCGC 2 cut(s) 879, 930
KroI GCCGGC 1 cut(s) 626
KroNI GCCGGC 1 cut(s) 628
Kzo9I GATC 4 cut(s) 174, 342, 1033, 1234
LmnI GCTCC 2 cut(s) 141, 238
Lsp1109I GCAGC 3 cut(s) 337, 625, 1145
LweI GCATC 3 cut(s) 328, 863, 1129
MaeI CTAG 2 cut(s) 90, 813
MaeII ACGT 3 cut(s) 77, 267, 291
MaeIII GTNAC 5 cut(s) 154, 292, 359, 836, 890
MalI GATC 4 cut(s) 176, 344, 1035, 1236
MboI GATC 4 cut(s) 174, 342, 1033, 1234
MboII GAAGA 2 cut(s) 697, 757
MfeI CAATTG 1 cut(s) 561
MflI RGATCY 2 cut(s) 174, 1234
MhlI GDGCHC 4 cut(s) 126, 235, 547, 956
MlsI TGGCCA 1 cut(s) 1160
MluCI AATT 6 cut(s) 243, 319, 561, 1069, 1148, 1177
MluNI TGGCCA 1 cut(s) 1160
MlyI GAGTC 2 cut(s) 367, 1110
MnlI CCTC 9 cut(s) 57, 63, 155, 235, 451, 853, 947, 1003, 1088
Mox20I TGGCCA 1 cut(s) 1160
Mph1103I ATGCAT 1 cut(s) 981
MreI CGCCGGCG 1 cut(s) 626
MroNI GCCGGC 1 cut(s) 626
MroXI GAANNNNTTC 1 cut(s) 8
MscI TGGCCA 1 cut(s) 1160
MseI TTAA 1 cut(s) 57
MslI CAYNNNNRTG 1 cut(s) 1267
Msp20I TGGCCA 1 cut(s) 1160
MspA1I CMGCKG 2 cut(s) 1136, 1232
MspI CCGG 6 cut(s) 483, 627, 649, 1007, 1074, 1112
MspR9I CCNGG 5 cut(s) 484, 671, 1080, 1113, 1215
MunI CAATTG 1 cut(s) 561
Mva1269I GAATGC 3 cut(s) 91, 719, 979
MvaI CCWGG 3 cut(s) 671, 1080, 1215
MvnI CGCG 1 cut(s) 932
MwoI GCNNNNNNNGC 7 cut(s) 230, 347, 501, 533, 542, 1024, 1139
NaeI GCCGGC 1 cut(s) 628
NciI CCSGG 2 cut(s) 484, 1113
NdeII GATC 4 cut(s) 174, 342, 1033, 1234
NgoMIV GCCGGC 1 cut(s) 626
NlaIII CATG 7 cut(s) 68, 114, 692, 926, 979, 1185, 1272
NlaIV GGNNCC 2 cut(s) 143, 1236
NmuCI GTSAC 3 cut(s) 292, 359, 890
NsiI ATGCAT 1 cut(s) 981
NspI RCATGY 3 cut(s) 68, 114, 692
PaeI GCATGC 1 cut(s) 692
PagI TCATGA 1 cut(s) 1268
PceI AGGCCT 1 cut(s) 224
PciI ACATGT 1 cut(s) 110
PctI GAATGC 3 cut(s) 91, 719, 979
PdiI GCCGGC 1 cut(s) 628
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 3 cut(s) 274, 521, 552
PfoI TCCNGGA 1 cut(s) 482
PinAI ACCGGT 1 cut(s) 648
PkrI GCNGC 6 cut(s) 352, 503, 640, 698, 1087, 1135
PleI GAGTC 2 cut(s) 366, 1109
PpsI GAGTC 2 cut(s) 366, 1109
PscI ACATGT 1 cut(s) 110
Psp6I CCWGG 3 cut(s) 669, 1078, 1213
PspEI GGTNACC 1 cut(s) 890
PspFI CCCAGC 1 cut(s) 897
PspGI CCWGG 3 cut(s) 669, 1078, 1213
PspN4I GGNNCC 2 cut(s) 143, 1236
PspPI GGNCC 2 cut(s) 257, 1076
PsuI RGATCY 2 cut(s) 174, 1234
PvuII CAGCTG 1 cut(s) 1136
RsaI GTAC 4 cut(s) 133, 205, 919, 935
RsaNI GTAC 4 cut(s) 132, 204, 918, 934
RseI CAYNNNNRTG 1 cut(s) 1267
SalI GTCGAC 2 cut(s) 73, 734
SaqAI TTAA 1 cut(s) 57
SatI GCNGC 6 cut(s) 351, 502, 639, 697, 1086, 1134
Sau3AI GATC 4 cut(s) 174, 342, 1033, 1234
Sau96I GGNCC 2 cut(s) 257, 1076
ScaI AGTACT 1 cut(s) 919
SchI GAGTC 2 cut(s) 367, 1110
ScrFI CCNGG 5 cut(s) 484, 671, 1080, 1113, 1215
SduI GDGCHC 4 cut(s) 126, 235, 547, 956
SfaNI GCATC 3 cut(s) 328, 863, 1129
SfcI CTRYAG 1 cut(s) 1038
SgrAI CRCCGGYG 2 cut(s) 626, 648
SinI GGWCC 1 cut(s) 257
SmiMI CAYNNNNRTG 1 cut(s) 1267
SmlI CTYRAG 2 cut(s) 546, 913
SmoI CTYRAG 2 cut(s) 546, 913
SphI GCATGC 1 cut(s) 692
Sse9I AATT 6 cut(s) 243, 319, 561, 1069, 1148, 1177
SseBI AGGCCT 1 cut(s) 224
SspI AATATT 1 cut(s) 1259
SspMI CTAG 2 cut(s) 90, 813
StuI AGGCCT 1 cut(s) 224
StyD4I CCNGG 5 cut(s) 482, 669, 1078, 1111, 1213
TaaI ACNGT 4 cut(s) 298, 733, 970, 1156
TaiI ACGT 3 cut(s) 80, 270, 294
TaqI TCGA 8 cut(s) 74, 126, 147, 251, 423, 735, 943, 1099
TaqII GACCGA 1 cut(s) 929
TasI AATT 6 cut(s) 243, 319, 561, 1069, 1148, 1177
TatI WGTACW 1 cut(s) 917
TauI GCSGC 3 cut(s) 504, 699, 1088
TfiI GAWTC 3 cut(s) 274, 521, 552
Tru1I TTAA 1 cut(s) 57
Tru9I TTAA 1 cut(s) 57
TscAI CASTG 6 cut(s) 301, 615, 763, 975, 1028, 1161
TseFI GTSAC 3 cut(s) 292, 359, 890
TseI GCWGC 3 cut(s) 350, 638, 1133
Tsp45I GTSAC 3 cut(s) 292, 359, 890
TspDTI ATGAA 8 cut(s) 17, 22, 43, 730, 964, 1109, 1170, 1251
TspRI CASTG 6 cut(s) 301, 615, 763, 975, 1028, 1161
VpaK11BI GGWCC 1 cut(s) 257
XagI CCTNNNNNAGG 1 cut(s) 1194
XapI RAATTY 4 cut(s) 319, 1069, 1148, 1177
XceI RCATGY 3 cut(s) 68, 114, 692
XmiI GTMKAC 3 cut(s) 74, 735, 1041
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 2 cut(s) 90, 813
ZrmI AGTACT 1 cut(s) 919
Zsp2I ATGCAT 1 cut(s) 981
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.