Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0001935

Physical Location & Seq

Genomic Coordinates Forward (+)

21887690 .. 21890514

Gene Structure 2825 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1105400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1497 bp

ATGAGTCCATTCATGAAGTCGTCCTACCAATTTATCATCGTTTCCACGATCTTGTTCTGTTCCCTATCCATTAACCCGGGTGCTAATAATGTCGATCCCCAGTTGATAGATGTTCCTTTGCCTGTGTTCAAAAATTCATTGGAGAAAACCATGGAAGCTGTGCAAAATGTAGCCTCAGCCATGTCACATATGCATGTTGGTGAAGATGCTAAAAATTCTACGAATATGATCATTTCATATTGTGAGGGTTTGCTTGGTGAAACTGTTGAAGTATTGAATTGGTCCCTTTCCACAATTCATGATTTGAAAGGTGATGTGGTTTCCCATATGAGAACATGGCTGAATACTTCTCATTACAGAGAAATGACATGCATGGAAGCTTTTAAGAATGGTATCGACTCAAGGGTTTCAGAGAGCCTCAAACATGCGACCAGACTAATCGATGAGGTCATCGGCATGATCCAAGTGCAGCAACACGATTACCAAAATGTCGATCAGCCTTTACTACTATCCGGTGGGACGAATTTCGAAGACGCAAAATTTTCCAAGACGCCAAATGTAACAGTTTCTCAGGATGGGAGTGGGAATTTTAATCGAATAATGGATGCCATTGCGGCTGCACCAAGTCATAGCCAGCAGCGGTTTGTGATATTTATCAAGAAAGGAATTTACAAAGAATATGTGAAAATTGATCCCACAAAGACTAATCTGGTCTTGATTGGAGAGGGCATGGACGTTACTACCATATCTGGCAACAGATGCAATGCTAGTGGTTGGCAAACAGTTGATTCAGCCACATTTGCTGTACGTGCTGAAGGATTCATAGCAATGTACGTAGGGTTTGAGAACACTGCGGGGCCAAATAAGTATCAGGCAGTTTCACTTTCATCCTACGGCGACCAATCAGTTTTCTACAGGTGCAAGATGAGTGGATATCAAGACACATTGCTGGTGCAGTCCGGTCGCCAGCTCTACCGAGAATGCACGATCAGTGGCACCATCGACTTCATCTTCGGCTACGGCACTGCCGTTTTCCAAAACTGTAAAATTCTTGCTAGGAAAAATCTTGAAGGTGCACAAAACACACTCACTGCGCATGGTCGATTTGCTGCAGATCCTTCAGGGTTCTCATTTCAATTTTGCAACGTTGAGCGGGACTCCGATCTAGGGGGTAATGTTGCTTCTTCGCCCACGTACTTAGGTCGACCATGGGGAAAATATTCACGTACTATATTCATGCAGTCATATATGAGTAATGTAATAGTGCCCGAGGGTTGGCTGGATTGGAATGGATTTGGTGGGCTTGGCACTTTATATTATGCGGAGTACATGAACAAAGGGGGTGCCTCGACTGTAGGTCGGGTCAAGTGGCCCGGCTATCATGTAATTGCCAAGGCATCCGAGGCCAAATTGTTTACCGTAACCAAGTTTATAGATGGTGACTCATGGCTGCCATCCACCGGTGTCCCATACGCACCAGGATTGGATCAAGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

499

Amino Acids

54.93

Weight (kDa)

6.08

Isoelectric Point (pI)

31.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	21 - 148	6e-11	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	186 - 481	4.6e-117	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001935)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g17310 FvH4_6g17430
malus_domestica	MD01G1105400.v1.1 MD03G1086400.v1.1 MD04G1225800.v1.1 MD06G1080700.v1.1
prunus_persica	Prupe.2G210700_v2.0.a1 Prupe.2G216300_v2.0.a1 Prupe.6G344600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0362901 RchiOBHm_Chr1g0362911 RchiOBHm_Chr3g0450671 RchiOBHm_Chr3g0471441
rosa_laevigata	RLG00000024178 RLG00000025712
rosa_multiflora	Rmu_co8224930.1_g000001 Rmu_co8324923.1_g000001 Rmu_sc0000078.1_g000035 Rmu_sc0001502.1_g000007 Rmu_sc0001502.1_g000008 Rmu_sc0006582.1_g000010
rosa_roxburghii	Rroxscaffold_4G00293660 Rroxscaffold_6G00409950 Rroxscaffold_6G00428330
rosa_rugosa	Rorug01G0302100 Rorug01G0302100 Rorug02G0629500 Rorug03G0117800
rosa_samantha	Rh1AG311400 Rh1AG311500 Rh1BG274600 Rh1CG291800 Rh1DG305300 Rh3AG030300 Rh3AG167600 Rh3BG030600 Rh3BG030700 Rh3BG193100 Rh3CG029500 Rh3CG183500 Rh3DG030200 Rh3DG030300 Rh3DG182400
rosa_wichuraiana	Rw1G027600 Rw3G002330 Rw3G015790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1095
AccB1I	GGYRCC	2 cut(s)	995, 1343
AccBSI	CCGCTC	1 cut(s)	1153
AccI	GTMKAC	1 cut(s)	1204
AciI	CCGC	5 cut(s)	614, 640, 854, 1153, 1322
AclI	AACGTT	1 cut(s)	1146
AclWI	GGATC	5 cut(s)	89, 454, 686, 1109, 1494
AcsI	RAATTY	7 cut(s)	133, 214, 523, 539, 586, 666, 1047
AcuI	CTGAAG	2 cut(s)	834, 1104
AcyI	GRCGYC	1 cut(s)	551
AfaI	GTAC	5 cut(s)	807, 833, 1196, 1228, 1328
AfiI	CCNNNNNNNGG	3 cut(s)	1167, 1275, 1460
AgeI	ACCGGT	1 cut(s)	1460
AgsI	TTSAA	6 cut(s)	130, 269, 277, 307, 1070, 1136
AhdI	GACNNNNNGTC	1 cut(s)	1356
AjnI	CCWGG	1 cut(s)	1477
AluBI	AGCT	3 cut(s)	158, 380, 970
AluI	AGCT	3 cut(s)	158, 380, 970
Alw21I	GWGCWC	1 cut(s)	1078
Alw44I	GTGCAC	1 cut(s)	1074
AlwI	GGATC	5 cut(s)	89, 454, 686, 1109, 1494
Ama87I	CYCGRG	2 cut(s)	76, 1268
AoxI	GGCC	3 cut(s)	857, 1370, 1404
ApaLI	GTGCAC	1 cut(s)	1074
ApeKI	GCWGC	5 cut(s)	469, 617, 637, 1109, 1450
ApoI	RAATTY	7 cut(s)	133, 214, 523, 539, 586, 666, 1047
ArsI	GACNNNNNNTTYG	2 cut(s)	995, 1027
AsiGI	ACCGGT	1 cut(s)	1460
Asp700I	GAANNNNTTC	1 cut(s)	1219
AspLEI	GCGC	1 cut(s)	1096
AspS9I	GGNCC	3 cut(s)	282, 857, 1371
AsuC2I	CCSGG	3 cut(s)	77, 78, 1374
AsuHPI	GGTGA	4 cut(s)	212, 269, 323, 1451
AsuII	TTCGAA	1 cut(s)	528
AvaI	CYCGRG	2 cut(s)	76, 1268
AvaII	GGWCC	1 cut(s)	282
BaeGI	GKGCMC	2 cut(s)	1078, 1269
BanI	GGYRCC	2 cut(s)	995, 1343
BarI	GAAGNNNNNNTAC	4 cut(s)	8, 40, 1165, 1197
BbsI	GAAGAC	1 cut(s)	537
Bbv12I	GWGCWC	1 cut(s)	1078
BbvCI	CCTCAGC	1 cut(s)	175
BbvI	GCAGC	5 cut(s)	481, 604, 649, 1096, 1437
BccI	CCATC	4 cut(s)	569, 1007, 1430, 1462
BceAI	ACGGC	3 cut(s)	910, 1013, 1036
BcgI	CGANNNNNNTGC	2 cut(s)	944, 978
BciT130I	CCWGG	1 cut(s)	1479
BclI	TGATCA	1 cut(s)	228
BcnI	CCSGG	3 cut(s)	77, 78, 1374
BfaI	CTAG	3 cut(s)	768, 1056, 1166
BfmI	CTRYAG	3 cut(s)	913, 1110, 1353
BglI	GCCNNNNNGGC	1 cut(s)	614
BisI	GCNGC	6 cut(s)	470, 615, 618, 638, 1110, 1451
BlsI	GCNGC	6 cut(s)	471, 616, 619, 639, 1111, 1452
Bme1390I	CCNGG	4 cut(s)	77, 78, 1374, 1479
Bme18I	GGWCC	1 cut(s)	282
BmeRI	GACNNNNNGTC	1 cut(s)	1356
BmeT110I	CYCGRG	2 cut(s)	76, 1268
BmgT120I	GGNCC	3 cut(s)	282, 857, 1371
BmiI	GGNNCC	4 cut(s)	284, 858, 997, 1345
BmrFI	CCNGG	4 cut(s)	77, 78, 1374, 1479
BmrI	ACTGGG	1 cut(s)	94
BmsI	GCATC	4 cut(s)	196, 595, 749, 1406
BmuI	ACTGGG	1 cut(s)	94
BpiI	GAAGAC	1 cut(s)	537
BplI	GAGNNNNNCTC	2 cut(s)	1142, 1174
Bpu10I	CCTNAGC	1 cut(s)	175
Bpu14I	TTCGAA	1 cut(s)	528
BpuEI	CTTGAG	1 cut(s)	385
BpuMI	CCSGG	3 cut(s)	77, 78, 1374
Bsa29I	ATCGAT	1 cut(s)	441
BsaAI	YACGTR	4 cut(s)	809, 835, 1194, 1226
BsaBI	GATNNNNATC	1 cut(s)	653
BsaHI	GRCGYC	1 cut(s)	551
BsaJI	CCNNGG	6 cut(s)	76, 150, 1208, 1269, 1392, 1401
BsaWI	WCCGGW	3 cut(s)	512, 959, 1460
Bsc4I	CCNNNNNNNGG	3 cut(s)	1167, 1275, 1460
Bse118I	RCCGGY	1 cut(s)	1460
Bse1I	ACTGG	1 cut(s)	100
Bse3DI	GCAATG	4 cut(s)	609, 769, 834, 944
Bse8I	GATNNNNATC	1 cut(s)	653
BseBI	CCWGG	1 cut(s)	1479
BseCI	ATCGAT	1 cut(s)	441
BseDI	CCNNGG	6 cut(s)	76, 150, 1208, 1269, 1392, 1401
BseGI	GGATG	5 cut(s)	580, 610, 887, 1397, 1454
BseJI	GATNNNNATC	1 cut(s)	653
BseLI	CCNNNNNNNGG	3 cut(s)	1167, 1275, 1460
BseMI	GCAATG	4 cut(s)	609, 769, 834, 944
BseMII	CTCAG	2 cut(s)	189, 584
BseNI	ACTGG	1 cut(s)	100
BseSI	GKGCMC	2 cut(s)	1078, 1269
BseXI	GCAGC	5 cut(s)	481, 604, 649, 1096, 1437
BsgI	GTGCAG	3 cut(s)	488, 603, 974
Bsh1285I	CGRYCG	1 cut(s)	964
BshFI	GGCC	3 cut(s)	859, 1372, 1406
BshNI	GGYRCC	2 cut(s)	995, 1343
BshTI	ACCGGT	1 cut(s)	1460
BshVI	ATCGAT	1 cut(s)	441
BsiEI	CGRYCG	1 cut(s)	964
BsiHKAI	GWGCWC	1 cut(s)	1078
BsiHKCI	CYCGRG	2 cut(s)	76, 1268
BsiSI	CCGG	5 cut(s)	77, 513, 960, 1374, 1461
BslFI	GGGAC	4 cut(s)	268, 532, 1169, 1451
BslI	CCNNNNNNNGG	3 cut(s)	1167, 1275, 1460
BsmFI	GGGAC	4 cut(s)	268, 532, 1169, 1451
BsmI	GAATGC	1 cut(s)	986
BsnI	GGCC	3 cut(s)	859, 1372, 1406
BsoBI	CYCGRG	2 cut(s)	76, 1268
Bsp119I	TTCGAA	1 cut(s)	528
Bsp1286I	GDGCHC	2 cut(s)	1078, 1269
Bsp19I	CCATGG	2 cut(s)	150, 1208
BspACI	CCGC	5 cut(s)	614, 640, 854, 1153, 1322
BspANI	GGCC	3 cut(s)	859, 1372, 1406
BspCNI	CTCAG	2 cut(s)	188, 583
BspDI	ATCGAT	1 cut(s)	441
BspHI	TCATGA	2 cut(s)	12, 298
BspLI	GGNNCC	4 cut(s)	284, 858, 997, 1345
BspMAI	CTGCAG	1 cut(s)	1114
BspPI	GGATC	5 cut(s)	89, 454, 686, 1109, 1494
BspT104I	TTCGAA	1 cut(s)	528
BspT107I	GGYRCC	2 cut(s)	995, 1343
BsrBI	CCGCTC	1 cut(s)	1153
BsrDI	GCAATG	4 cut(s)	609, 769, 834, 944
BsrFI	RCCGGY	1 cut(s)	1460
BsrI	ACTGG	1 cut(s)	100
BssAI	RCCGGY	1 cut(s)	1460
BssECI	CCNNGG	6 cut(s)	76, 150, 1208, 1269, 1392, 1401
BssNI	GRCGYC	1 cut(s)	551
BssT1I	CCWWGG	3 cut(s)	150, 1208, 1392
Bst2UI	CCWGG	1 cut(s)	1479
Bst4CI	ACNGT	6 cut(s)	265, 565, 784, 1043, 1354, 1420
BstACI	GRCGYC	1 cut(s)	551
BstAPI	GCANNNNNTGC	1 cut(s)	759
BstBAI	YACGTR	4 cut(s)	809, 835, 1194, 1226
BstBI	TTCGAA	1 cut(s)	528
BstC8I	GCNNGC	2 cut(s)	635, 968
BstDEI	CTNAG	3 cut(s)	175, 570, 1198
BstDSI	CCRYGG	2 cut(s)	150, 1208
BstF5I	GGATG	5 cut(s)	580, 610, 887, 1397, 1454
BstHHI	GCGC	1 cut(s)	1096
BstMCI	CGRYCG	1 cut(s)	964
BstMWI	GCNNNNNNNGC	5 cut(s)	614, 759, 800, 809, 1403
BstNI	CCWGG	1 cut(s)	1479
BstNSI	RCATGY	3 cut(s)	197, 372, 428
BstSCI	CCNGG	4 cut(s)	75, 76, 1372, 1477
BstSFI	CTRYAG	3 cut(s)	913, 1110, 1353
BstSLI	GKGCMC	2 cut(s)	1078, 1269
BstSNI	TACGTA	1 cut(s)	835
BstV1I	GCAGC	5 cut(s)	481, 604, 649, 1096, 1437
BstV2I	GAAGAC	1 cut(s)	537
BstX2I	RGATCY	1 cut(s)	1114
BstYI	RGATCY	1 cut(s)	1114
Bsu15I	ATCGAT	1 cut(s)	441
BsuRI	GGCC	3 cut(s)	859, 1372, 1406
BsuTUI	ATCGAT	1 cut(s)	441
BtgI	CCRYGG	2 cut(s)	150, 1208
BtsCI	GGATG	5 cut(s)	580, 610, 887, 1397, 1454
BtsI	GCAGTG	3 cut(s)	849, 1023, 1089
BtsIMutI	CAGTG	4 cut(s)	849, 997, 1023, 1089
Cac8I	GCNNGC	2 cut(s)	635, 968
CciI	TCATGA	2 cut(s)	12, 298
CfoI	GCGC	1 cut(s)	1096
Cfr10I	RCCGGY	1 cut(s)	1460
Cfr13I	GGNCC	3 cut(s)	282, 857, 1371
Cfr9I	CCCGGG	1 cut(s)	76
ClaI	ATCGAT	1 cut(s)	441
CseI	GACGC	2 cut(s)	542, 559
Csp6I	GTAC	5 cut(s)	806, 832, 1195, 1227, 1327
CspAI	ACCGGT	1 cut(s)	1460
CspCI	CAANNNNNGTGG	4 cut(s)	751, 786, 910, 945
CviQI	GTAC	5 cut(s)	806, 832, 1195, 1227, 1327
DdeI	CTNAG	3 cut(s)	175, 570, 1198
DriI	GACNNNNNGTC	1 cut(s)	1356
Eam1105I	GACNNNNNGTC	1 cut(s)	1356
Eco105I	TACGTA	1 cut(s)	835
Eco130I	CCWWGG	3 cut(s)	150, 1208, 1392
Eco32I	GATATC	1 cut(s)	935
Eco47I	GGWCC	1 cut(s)	282
Eco57I	CTGAAG	2 cut(s)	834, 1104
Eco88I	CYCGRG	2 cut(s)	76, 1268
EcoRII	CCWGG	1 cut(s)	1477
EcoRV	GATATC	1 cut(s)	935
EcoT14I	CCWWGG	3 cut(s)	150, 1208, 1392
EcoT22I	ATGCAT	2 cut(s)	195, 374
ErhI	CCWWGG	3 cut(s)	150, 1208, 1392
FaqI	GGGAC	4 cut(s)	268, 532, 1169, 1451
FauI	CCCGC	2 cut(s)	847, 1146
FauNDI	CATATG	2 cut(s)	189, 327
FbaI	TGATCA	1 cut(s)	228
FblI	GTMKAC	1 cut(s)	1204
Fnu4HI	GCNGC	6 cut(s)	470, 615, 618, 638, 1110, 1451
FokI	GGATG	5 cut(s)	587, 617, 874, 1384, 1441
Fsp4HI	GCNGC	6 cut(s)	470, 615, 618, 638, 1110, 1451
FspBI	CTAG	3 cut(s)	768, 1056, 1166
FspI	TGCGCA	1 cut(s)	1095
GlaI	GCGC	1 cut(s)	1095
GluI	GCNGC	6 cut(s)	470, 615, 618, 638, 1110, 1451
HaeIII	GGCC	3 cut(s)	859, 1372, 1406
HapII	CCGG	5 cut(s)	77, 513, 960, 1374, 1461
HgaI	GACGC	2 cut(s)	542, 559
HhaI	GCGC	1 cut(s)	1096
Hin1I	GRCGYC	1 cut(s)	551
Hin6I	GCGC	1 cut(s)	1094
HinP1I	GCGC	1 cut(s)	1094
HincII	GTYRAC	1 cut(s)	1205
HindII	GTYRAC	1 cut(s)	1205
HindIII	AAGCTT	1 cut(s)	378
HinfI	GANTC	6 cut(s)	4, 398, 788, 819, 1157, 1442
HpaII	CCGG	5 cut(s)	77, 513, 960, 1374, 1461
HphI	GGTGA	4 cut(s)	212, 269, 323, 1451
Hpy166II	GTNNAC	3 cut(s)	1076, 1205, 1416
Hpy188I	TCNGA	3 cut(s)	412, 1162, 1402
Hpy188III	TCNNGA	7 cut(s)	13, 299, 572, 658, 715, 938, 1067
Hpy8I	GTNNAC	3 cut(s)	1076, 1205, 1416
HpyAV	CCTTC	3 cut(s)	809, 1064, 1128
HpyCH4III	ACNGT	6 cut(s)	265, 565, 784, 1043, 1354, 1420
HpyCH4IV	ACGT	6 cut(s)	735, 808, 834, 1146, 1193, 1225
HpyF10VI	GCNNNNNNNGC	5 cut(s)	614, 759, 800, 809, 1403
HpyF3I	CTNAG	3 cut(s)	175, 570, 1198
HpySE526I	ACGT	6 cut(s)	735, 808, 834, 1146, 1193, 1225
Hsp92I	GRCGYC	1 cut(s)	551
HspAI	GCGC	1 cut(s)	1094
Ksp22I	TGATCA	1 cut(s)	228
Lsp1109I	GCAGC	5 cut(s)	481, 604, 649, 1096, 1437
LweI	GCATC	4 cut(s)	196, 595, 749, 1406
MaeI	CTAG	3 cut(s)	768, 1056, 1166
MaeII	ACGT	6 cut(s)	735, 808, 834, 1146, 1193, 1225
MaeIII	GTNAC	5 cut(s)	183, 559, 736, 1420, 1439
MbiI	CCGCTC	1 cut(s)	1153
MboII	GAAGA	4 cut(s)	215, 542, 1003, 1176
MflI	RGATCY	1 cut(s)	1114
MhlI	GDGCHC	2 cut(s)	1078, 1269
MlyI	GAGTC	4 cut(s)	13, 392, 1151, 1436
MnlI	CCTC	8 cut(s)	184, 238, 428, 439, 718, 1264, 1357, 1396
Mph1103I	ATGCAT	2 cut(s)	195, 374
MroXI	GAANNNNTTC	1 cut(s)	1219
MseI	TTAA	3 cut(s)	72, 384, 591
MslI	CAYNNNNRTG	4 cut(s)	192, 198, 455, 827
MspA1I	CMGCKG	1 cut(s)	640
MspI	CCGG	5 cut(s)	77, 513, 960, 1374, 1461
MspR9I	CCNGG	4 cut(s)	77, 78, 1374, 1479
Mva1269I	GAATGC	1 cut(s)	986
MvaI	CCWGG	1 cut(s)	1479
MwoI	GCNNNNNNNGC	5 cut(s)	614, 759, 800, 809, 1403
NciI	CCSGG	3 cut(s)	77, 78, 1374
NcoI	CCATGG	2 cut(s)	150, 1208
NdeI	CATATG	2 cut(s)	189, 327
NlaIV	GGNNCC	4 cut(s)	284, 858, 997, 1345
NmuCI	GTSAC	2 cut(s)	183, 1439
NsbI	TGCGCA	1 cut(s)	1095
NsiI	ATGCAT	2 cut(s)	195, 374
NspI	RCATGY	3 cut(s)	197, 372, 428
NspV	TTCGAA	1 cut(s)	528
PagI	TCATGA	2 cut(s)	12, 298
PcsI	WCGNNNNNNNCGW	1 cut(s)	1026
PctI	GAATGC	1 cut(s)	986
PdmI	GAANNNNTTC	1 cut(s)	1219
PfeI	GAWTC	2 cut(s)	788, 819
PinAI	ACCGGT	1 cut(s)	1460
PkrI	GCNGC	6 cut(s)	471, 616, 619, 639, 1111, 1452
PleI	GAGTC	4 cut(s)	12, 392, 1151, 1436
PpsI	GAGTC	4 cut(s)	12, 392, 1151, 1436
Ppu21I	YACGTR	4 cut(s)	809, 835, 1194, 1226
Psp1406I	AACGTT	1 cut(s)	1146
Psp6I	CCWGG	1 cut(s)	1477
PspGI	CCWGG	1 cut(s)	1477
PspN4I	GGNNCC	4 cut(s)	284, 858, 997, 1345
PspPI	GGNCC	3 cut(s)	282, 857, 1371
PstI	CTGCAG	1 cut(s)	1114
PsuI	RGATCY	1 cut(s)	1114
RsaI	GTAC	5 cut(s)	807, 833, 1196, 1228, 1328
RsaNI	GTAC	5 cut(s)	806, 832, 1195, 1227, 1327
RseI	CAYNNNNRTG	4 cut(s)	192, 198, 455, 827
SalI	GTCGAC	1 cut(s)	1203
SaqAI	TTAA	3 cut(s)	72, 384, 591
SatI	GCNGC	6 cut(s)	470, 615, 618, 638, 1110, 1451
Sau96I	GGNCC	3 cut(s)	282, 857, 1371
SchI	GAGTC	4 cut(s)	13, 392, 1151, 1436
ScrFI	CCNGG	4 cut(s)	77, 78, 1374, 1479
SduI	GDGCHC	2 cut(s)	1078, 1269
SfaNI	GCATC	4 cut(s)	196, 595, 749, 1406
SfcI	CTRYAG	3 cut(s)	913, 1110, 1353
SfuI	TTCGAA	1 cut(s)	528
SgrAI	CRCCGGYG	1 cut(s)	1460
SinI	GGWCC	1 cut(s)	282
SmaI	CCCGGG	1 cut(s)	78
SmiMI	CAYNNNNRTG	4 cut(s)	192, 198, 455, 827
SmlI	CTYRAG	1 cut(s)	400
SmoI	CTYRAG	1 cut(s)	400
SnaBI	TACGTA	1 cut(s)	835
SsiI	CCGC	5 cut(s)	614, 640, 854, 1153, 1322
SspI	AATATT	1 cut(s)	1220
SspMI	CTAG	3 cut(s)	768, 1056, 1166
StyD4I	CCNGG	4 cut(s)	75, 76, 1372, 1477
StyI	CCWWGG	3 cut(s)	150, 1208, 1392
TaaI	ACNGT	6 cut(s)	265, 565, 784, 1043, 1354, 1420
TaiI	ACGT	6 cut(s)	738, 811, 837, 1149, 1196, 1228
TatI	WGTACW	1 cut(s)	1326
TauI	GCSGC	1 cut(s)	617
TfiI	GAWTC	2 cut(s)	788, 819
Tru1I	TTAA	3 cut(s)	72, 384, 591
Tru9I	TTAA	3 cut(s)	72, 384, 591
TscAI	CASTG	4 cut(s)	856, 997, 1030, 1096
TseFI	GTSAC	2 cut(s)	183, 1439
TseI	GCWGC	5 cut(s)	469, 617, 637, 1109, 1450
Tsp45I	GTSAC	2 cut(s)	183, 1439
TspDTI	ATGAA	9 cut(s)	29, 126, 225, 287, 811, 876, 997, 1225, 1346
TspMI	CCCGGG	1 cut(s)	76
TspRI	CASTG	4 cut(s)	856, 997, 1030, 1096
VneI	GTGCAC	1 cut(s)	1074
VpaK11BI	GGWCC	1 cut(s)	282
XapI	RAATTY	7 cut(s)	133, 214, 523, 539, 586, 666, 1047
XceI	RCATGY	3 cut(s)	197, 372, 428
XmaI	CCCGGG	1 cut(s)	76
XmiI	GTMKAC	1 cut(s)	1204
XmnI	GAANNNNTTC	1 cut(s)	1219
XspI	CTAG	3 cut(s)	768, 1056, 1166
Zsp2I	ATGCAT	2 cut(s)	195, 374

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.