Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0001935

Physical Location & Seq

Genomic Coordinates Forward (+)

7068799 .. 7069263

Gene Structure 465 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1086400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 465 bp

ATGATGAAACCTTGGCTCAATGACTTTGTATTAAGATCAAGAGCCTCCCTAGCCATTGTGGTTGCAATTTCCGATCTATCAAAGTCAAACCCGATTAAGGAGCTGCTGGATGGGATTTTTCCGACTTGGGTCACAAGCAAGGACAGAAGGCTTTTGCTGGATTCTAATTTGGCAAAGGAAAGTAATGCTAATGTTGTGGTTGCAAAGGATGAGAGTGGGAACTATAAGACTGTGAAAGAAGCAGTGGCAGCAGCACCAGATAATAGCAAAACCAGGTACACTATTCATGTGAAAAAAGGAACTTACAAGGAAAATGTCGAGGTCGGGAAGAAAAAGAAGAACTTGATGATTACTAAAGATGGCATGCATAACACAATCATCACTGATAGCCTCAATGTTGTTGATGAAGCAACCACTTTCAACTCCACAACTTTAGCTGGAGTACACCAGCTTGTGTTCAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0016020 GO:0016787 GO:0016788 GO:0030234 GO:0030312 GO:0030599 GO:0031090 GO:0043086 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044092 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046910 GO:0050790 GO:0050896 GO:0051704 GO:0051707 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098588 GO:0098772 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

155

Amino Acids

17.06

Weight (kDa)

9.49

Isoelectric Point (pI)

21.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pectinesterase	PF01095	64 - 148	5.2e-27	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0001935)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g17310 FvH4_6g17430
malus_domestica	MD01G1105400.v1.1 MD03G1086400.v1.1 MD04G1225800.v1.1 MD06G1080700.v1.1
prunus_persica	Prupe.2G210700_v2.0.a1 Prupe.2G216300_v2.0.a1 Prupe.6G344600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0362901 RchiOBHm_Chr1g0362911 RchiOBHm_Chr3g0450671 RchiOBHm_Chr3g0471441
rosa_laevigata	RLG00000024178 RLG00000025712
rosa_multiflora	Rmu_co8224930.1_g000001 Rmu_co8324923.1_g000001 Rmu_sc0000078.1_g000035 Rmu_sc0001502.1_g000007 Rmu_sc0001502.1_g000008 Rmu_sc0006582.1_g000010
rosa_roxburghii	Rroxscaffold_4G00293660 Rroxscaffold_6G00409950 Rroxscaffold_6G00428330
rosa_rugosa	Rorug01G0302100 Rorug01G0302100 Rorug02G0629500 Rorug03G0117800
rosa_samantha	Rh1AG311400 Rh1AG311500 Rh1BG274600 Rh1CG291800 Rh1DG305300 Rh3AG030300 Rh3AG167600 Rh3BG030600 Rh3BG030700 Rh3BG193100 Rh3CG029500 Rh3CG183500 Rh3DG030200 Rh3DG030300 Rh3DG182400
rosa_wichuraiana	Rw1G027600 Rw3G002330 Rw3G015790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AfaI	GTAC	2 cut(s)	278, 444
AfiI	CCNNNNNNNGG	1 cut(s)	97
AgsI	TTSAA	2 cut(s)	421, 460
AjnI	CCWGG	1 cut(s)	272
AluBI	AGCT	3 cut(s)	103, 437, 451
AluI	AGCT	3 cut(s)	103, 437, 451
ApeKI	GCWGC	3 cut(s)	103, 248, 251
BbvI	GCAGC	3 cut(s)	90, 260, 263
BccI	CCATC	2 cut(s)	104, 353
BciT130I	CCWGG	1 cut(s)	274
BfaI	CTAG	1 cut(s)	50
BisI	GCNGC	3 cut(s)	104, 249, 252
BlsI	GCNGC	3 cut(s)	105, 250, 253
Bme1390I	CCNGG	1 cut(s)	274
BmrFI	CCNGG	1 cut(s)	274
BoxI	GACNNNNGTC	1 cut(s)	128
BpmI	CTGGAG	1 cut(s)	459
BsaJI	CCNNGG	1 cut(s)	11
Bsc4I	CCNNNNNNNGG	1 cut(s)	97
BseBI	CCWGG	1 cut(s)	274
BseDI	CCNNGG	1 cut(s)	11
BseGI	GGATG	2 cut(s)	115, 214
BseLI	CCNNNNNNNGG	1 cut(s)	97
BseXI	GCAGC	3 cut(s)	90, 260, 263
BslI	CCNNNNNNNGG	1 cut(s)	97
Bsp143I	GATC	2 cut(s)	35, 73
BssECI	CCNNGG	1 cut(s)	11
BssMI	GATC	2 cut(s)	35, 73
BssT1I	CCWWGG	1 cut(s)	11
Bst2UI	CCWGG	1 cut(s)	274
Bst4CI	ACNGT	1 cut(s)	232
BstC8I	GCNNGC	1 cut(s)	365
BstF5I	GGATG	2 cut(s)	115, 214
BstKTI	GATC	2 cut(s)	38, 76
BstMBI	GATC	2 cut(s)	35, 73
BstMWI	GCNNNNNNNGC	2 cut(s)	50, 248
BstNI	CCWGG	1 cut(s)	274
BstNSI	RCATGY	1 cut(s)	367
BstPAI	GACNNNNGTC	1 cut(s)	128
BstSCI	CCNGG	1 cut(s)	272
BstV1I	GCAGC	3 cut(s)	90, 260, 263
BtsCI	GGATG	2 cut(s)	115, 214
BtsI	GCAGTG	1 cut(s)	249
BtsIMutI	CAGTG	2 cut(s)	249, 381
Cac8I	GCNNGC	1 cut(s)	365
CsiI	ACCWGGT	1 cut(s)	272
Csp6I	GTAC	2 cut(s)	277, 443
CviAII	CATG	2 cut(s)	287, 364
CviJI	RGCY	8 cut(s)	16, 44, 53, 103, 151, 390, 437, 451
CviKI_1	RGCY	8 cut(s)	16, 44, 53, 103, 151, 390, 437, 451
CviQI	GTAC	2 cut(s)	277, 443
DpnI	GATC	2 cut(s)	37, 75
DpnII	GATC	2 cut(s)	35, 73
Eco130I	CCWWGG	1 cut(s)	11
EcoRII	CCWGG	1 cut(s)	272
EcoT14I	CCWWGG	1 cut(s)	11
EcoT22I	ATGCAT	1 cut(s)	369
ErhI	CCWWGG	1 cut(s)	11
FaeI	CATG	2 cut(s)	290, 367
FaiI	YATR	4 cut(s)	225, 288, 365, 369
FalI	AAGNNNNNCTT	2 cut(s)	326, 358
FatI	CATG	2 cut(s)	286, 363
Fnu4HI	GCNGC	3 cut(s)	104, 249, 252
FokI	GGATG	2 cut(s)	122, 221
Fsp4HI	GCNGC	3 cut(s)	104, 249, 252
FspBI	CTAG	1 cut(s)	50
GluI	GCNGC	3 cut(s)	104, 249, 252
GsuI	CTGGAG	1 cut(s)	459
Hin1II	CATG	2 cut(s)	290, 367
HinfI	GANTC	1 cut(s)	161
Hpy166II	GTNNAC	2 cut(s)	279, 445
Hpy188I	TCNGA	2 cut(s)	73, 123
Hpy188III	TCNNGA	2 cut(s)	39, 325
Hpy8I	GTNNAC	2 cut(s)	279, 445
HpyAV	CCTTC	1 cut(s)	141
HpyCH4III	ACNGT	1 cut(s)	232
HpyCH4V	TGCA	3 cut(s)	65, 203, 367
HpyF10VI	GCNNNNNNNGC	2 cut(s)	50, 248
Hsp92II	CATG	2 cut(s)	290, 367
Kzo9I	GATC	2 cut(s)	35, 73
LmnI	GCTCC	1 cut(s)	100
LpnPI	CCDG	7 cut(s)	92, 143, 259, 270, 286, 423, 461
Lsp1109I	GCAGC	3 cut(s)	90, 260, 263
MabI	ACCWGGT	1 cut(s)	272
MaeI	CTAG	1 cut(s)	50
MaeIII	GTNAC	1 cut(s)	130
MalI	GATC	2 cut(s)	37, 75
MboI	GATC	2 cut(s)	35, 73
MboII	GAAGA	2 cut(s)	340, 349
MluCI	AATT	2 cut(s)	66, 166
MmeI	TCCRAC	1 cut(s)	146
MnlI	CCTC	3 cut(s)	55, 313, 401
Mph1103I	ATGCAT	1 cut(s)	369
MseI	TTAA	2 cut(s)	32, 96
MspR9I	CCNGG	1 cut(s)	274
MvaI	CCWGG	1 cut(s)	274
MwoI	GCNNNNNNNGC	2 cut(s)	50, 248
NdeII	GATC	2 cut(s)	35, 73
NlaIII	CATG	2 cut(s)	290, 367
NmuCI	GTSAC	1 cut(s)	130
NsiI	ATGCAT	1 cut(s)	369
NspI	RCATGY	1 cut(s)	367
PaeI	GCATGC	1 cut(s)	367
PfeI	GAWTC	1 cut(s)	161
PkrI	GCNGC	3 cut(s)	105, 250, 253
PshAI	GACNNNNGTC	1 cut(s)	128
Psp6I	CCWGG	1 cut(s)	272
PspGI	CCWGG	1 cut(s)	272
RsaI	GTAC	2 cut(s)	278, 444
RsaNI	GTAC	2 cut(s)	277, 443
SaqAI	TTAA	2 cut(s)	32, 96
SatI	GCNGC	3 cut(s)	104, 249, 252
Sau3AI	GATC	2 cut(s)	35, 73
ScrFI	CCNGG	1 cut(s)	274
SetI	ASST	6 cut(s)	13, 105, 278, 324, 439, 453
SexAI	ACCWGGT	1 cut(s)	272
SphI	GCATGC	1 cut(s)	367
Sse9I	AATT	2 cut(s)	66, 166
SspMI	CTAG	1 cut(s)	50
StyD4I	CCNGG	1 cut(s)	272
StyI	CCWWGG	1 cut(s)	11
TaaI	ACNGT	1 cut(s)	232
TaqI	TCGA	1 cut(s)	318
TasI	AATT	2 cut(s)	66, 166
TatI	WGTACW	1 cut(s)	442
TfiI	GAWTC	1 cut(s)	161
Tru1I	TTAA	2 cut(s)	32, 96
Tru9I	TTAA	2 cut(s)	32, 96
TscAI	CASTG	2 cut(s)	249, 388
TseFI	GTSAC	1 cut(s)	130
TseI	GCWGC	3 cut(s)	103, 248, 251
Tsp45I	GTSAC	1 cut(s)	130
TspDTI	ATGAA	3 cut(s)	20, 275, 420
TspRI	CASTG	2 cut(s)	249, 388
XceI	RCATGY	1 cut(s)	367
XspI	CTAG	1 cut(s)	50
Zsp2I	ATGCAT	1 cut(s)	369

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.