Rh1DG305300

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0001935
Physical Location & Seq
Forward (+)
53268573 .. 53269487
915 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG305300.1

Sequence Viewer

Length: 780 bp
ATGTTGCCTGAGCTGCCTAATGCTCTTGATCTAGAACCTAGATCACATCAGACTCAGTTCCAGCGAATCAGTTATTGGAAGAATAACCCTAACGTCATCGTGTCTAAGGATTGGACTGGAAATTTCCAAAAGATAATGCAGGCAGTAGAGGCTGTACCGGATCATAGCCGGAAGCGTTTTGTGATCTTGGTGAAGAGAGGAGTTTACAAGGAAAATGTGGATATCAATGTGAAAAAGACTAATTTGGTTTTGATTGGAGAGGGCATGGACGTTACTACCATAACTGGACATAAGAGTAATGGTACTGGTTGGCGTAAATTGTCCACACTTTCTATCATACATGGCTACTTTTATAACATACATGATGTTCACTCTTTCAGTTCTGCAATTCATAACCAATTATTTTTTGTATGTGAATCAATGTTGTGCATAGCTGTGAAGGCAGAAGGCTTCATTGCAATAGATATAGGGTTTGAGAACACAGCCGGACCACACAACGGCCAGGCAGTGGCACTTCTAGTCAAGGGTGACCAATCAGCTTTCTTCCGATGCAAGATAAGTGGATACCAAGACACATTGTATGCACACAACAGCCGCCAGTTCTTTAGGGAATGCAAGATCAGTGGCACTGTCGACTTCATCTTCGGCTACAGCACTGCAGTTTTCCAAATGTGCACCATTATTGCAAGAAAAGACCTAATCATGGCCTGGTTACAGTTACTGCCCACGGTCGTTTCGAAGAAGACTCAACCGGGTTTACATTCCAATTGTGTGACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
PMEI Pectinesterase

Protein Analysis

259

Amino Acids

29.19

Weight (kDa)

9.1

Isoelectric Point (pI)

24.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 31 - 110 4.3e-22 Pectinesterase
Pectinesterase PF01095 143 - 232 2.7e-41 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 354
AccB7I CCANNNNNTGG 1 cut(s) 508
AccI GTMKAC 1 cut(s) 633
AciI CCGC 1 cut(s) 595
AclWI GGATC 1 cut(s) 168
AcoI YGGCCR 1 cut(s) 499
AcsI RAATTY 1 cut(s) 121
AfaI GTAC 2 cut(s) 156, 304
AfiI CCNNNNNNNGG 3 cut(s) 497, 508, 703
AjiI CACGTC 1 cut(s) 777
AjnI CCWGG 2 cut(s) 501, 707
AjuI GAANNNNNNNTTGG 2 cut(s) 58, 90
AluBI AGCT 3 cut(s) 13, 434, 539
AluI AGCT 3 cut(s) 13, 434, 539
Alw21I GWGCWC 1 cut(s) 677
Alw44I GTGCAC 1 cut(s) 673
AlwI GGATC 1 cut(s) 168
AlwNI CAGNNNCTG 1 cut(s) 721
AoxI GGCC 2 cut(s) 499, 705
ApaLI GTGCAC 1 cut(s) 673
ApeKI GCWGC 1 cut(s) 13
ApoI RAATTY 1 cut(s) 121
ArsI GACNNNNNNTTYG 2 cut(s) 626, 658
AspS9I GGNCC 1 cut(s) 488
AsuC2I CCSGG 1 cut(s) 753
AsuHPI GGTGA 2 cut(s) 202, 539
AsuII TTCGAA 1 cut(s) 737
AvaII GGWCC 1 cut(s) 488
BaeGI GKGCMC 1 cut(s) 677
BbsI GAAGAC 1 cut(s) 749
Bbv12I GWGCWC 1 cut(s) 677
BceAI ACGGC 1 cut(s) 514
BciT130I CCWGG 2 cut(s) 503, 709
BciVI GTATCC 1 cut(s) 557
BcnI CCSGG 1 cut(s) 753
BfaI CTAG 3 cut(s) 32, 39, 518
BfmI CTRYAG 2 cut(s) 649, 657
BfuI GTATCC 1 cut(s) 557
BisI GCNGC 2 cut(s) 14, 595
BlsI GCNGC 2 cut(s) 15, 596
Bme1390I CCNGG 3 cut(s) 503, 709, 753
Bme18I GGWCC 1 cut(s) 488
BmgBI CACGTC 1 cut(s) 777
BmgT120I GGNCC 1 cut(s) 488
BmrFI CCNGG 3 cut(s) 503, 709, 753
BmsI GCATC 1 cut(s) 539
BpiI GAAGAC 1 cut(s) 749
Bpu10I CCTNAGC 1 cut(s) 9
Bpu14I TTCGAA 1 cut(s) 737
BpuMI CCSGG 1 cut(s) 753
BsaJI CCNNGG 1 cut(s) 726
BsaWI WCCGGW 1 cut(s) 157
Bsc4I CCNNNNNNNGG 3 cut(s) 497, 508, 703
Bse1I ACTGG 4 cut(s) 121, 289, 310, 598
Bse3DI GCAATG 1 cut(s) 453
BseBI CCWGG 2 cut(s) 503, 709
BseDI CCNNGG 1 cut(s) 726
BseLI CCNNNNNNNGG 3 cut(s) 497, 508, 703
BseMI GCAATG 1 cut(s) 453
BseMII CTCAG 1 cut(s) 68
BseNI ACTGG 4 cut(s) 121, 289, 310, 598
BseRI GAGGAG 1 cut(s) 213
BseSI GKGCMC 1 cut(s) 677
Bsh1285I CGRYCG 1 cut(s) 732
BshFI GGCC 2 cut(s) 501, 707
BsiEI CGRYCG 1 cut(s) 732
BsiHKAI GWGCWC 1 cut(s) 677
BsiSI CCGG 4 cut(s) 158, 169, 486, 752
BslI CCNNNNNNNGG 3 cut(s) 497, 508, 703
BsmI GAATGC 1 cut(s) 617
BsnI GGCC 2 cut(s) 501, 707
Bsp119I TTCGAA 1 cut(s) 737
Bsp1286I GDGCHC 1 cut(s) 677
Bsp143I GATC 5 cut(s) 28, 41, 160, 183, 618
BspACI CCGC 1 cut(s) 595
BspANI GGCC 2 cut(s) 501, 707
BspCNI CTCAG 1 cut(s) 67
BspMAI CTGCAG 1 cut(s) 661
BspPI GGATC 1 cut(s) 168
BspT104I TTCGAA 1 cut(s) 737
BsrDI GCAATG 1 cut(s) 453
BsrI ACTGG 4 cut(s) 121, 289, 310, 598
BssECI CCNNGG 1 cut(s) 726
BssMI GATC 5 cut(s) 28, 41, 160, 183, 618
Bst2UI CCWGG 2 cut(s) 503, 709
Bst4CI ACNGT 3 cut(s) 631, 717, 730
Bst6I CTCTTC 1 cut(s) 188
BstBI TTCGAA 1 cut(s) 737
BstC8I GCNNGC 1 cut(s) 141
BstDEI CTNAG 3 cut(s) 9, 54, 105
BstDSI CCRYGG 1 cut(s) 726
BstEII GGTNACC 1 cut(s) 527
BstKTI GATC 5 cut(s) 31, 44, 163, 186, 621
BstMBI GATC 5 cut(s) 28, 41, 160, 183, 618
BstMCI CGRYCG 1 cut(s) 732
BstMWI GCNNNNNNNGC 3 cut(s) 13, 149, 440
BstNI CCWGG 2 cut(s) 503, 709
BstPI GGTNACC 1 cut(s) 527
BstSCI CCNGG 3 cut(s) 501, 707, 751
BstSFI CTRYAG 2 cut(s) 649, 657
BstSLI GKGCMC 1 cut(s) 677
BstV2I GAAGAC 1 cut(s) 749
BsuI GTATCC 1 cut(s) 557
BsuRI GGCC 2 cut(s) 501, 707
BtgI CCRYGG 1 cut(s) 726
BtrI CACGTC 1 cut(s) 777
BtsI GCAGTG 2 cut(s) 513, 654
BtsIMutI CAGTG 4 cut(s) 513, 627, 628, 654
Cac8I GCNNGC 1 cut(s) 141
CaiI CAGNNNCTG 1 cut(s) 721
Cfr13I GGNCC 1 cut(s) 488
Csp6I GTAC 2 cut(s) 155, 303
CspCI CAANNNNNGTGG 4 cut(s) 541, 576, 604, 639
CviAII CATG 4 cut(s) 265, 341, 362, 703
CviQI GTAC 2 cut(s) 155, 303
DdeI CTNAG 3 cut(s) 9, 54, 105
DpnI GATC 5 cut(s) 30, 43, 162, 185, 620
DpnII GATC 5 cut(s) 28, 41, 160, 183, 618
EaeI YGGCCR 1 cut(s) 499
Eam1104I CTCTTC 1 cut(s) 188
EarI CTCTTC 1 cut(s) 188
Eco32I GATATC 1 cut(s) 223
Eco47I GGWCC 1 cut(s) 488
Eco91I GGTNACC 1 cut(s) 527
EcoO65I GGTNACC 1 cut(s) 527
EcoRII CCWGG 2 cut(s) 501, 707
EcoRV GATATC 1 cut(s) 223
FaeI CATG 4 cut(s) 268, 344, 365, 706
FatI CATG 4 cut(s) 264, 340, 361, 702
FblI GTMKAC 1 cut(s) 633
Fnu4HI GCNGC 2 cut(s) 14, 595
Fsp4HI GCNGC 2 cut(s) 14, 595
FspBI CTAG 3 cut(s) 32, 39, 518
GluI GCNGC 2 cut(s) 14, 595
HaeIII GGCC 2 cut(s) 501, 707
HapII CCGG 4 cut(s) 158, 169, 486, 752
Hin1II CATG 4 cut(s) 268, 344, 365, 706
HincII GTYRAC 1 cut(s) 634
HindII GTYRAC 1 cut(s) 634
HinfI GANTC 4 cut(s) 52, 66, 416, 745
HpaII CCGG 4 cut(s) 158, 169, 486, 752
HphI GGTGA 2 cut(s) 202, 539
Hpy166II GTNNAC 6 cut(s) 205, 324, 370, 634, 675, 758
Hpy188I TCNGA 2 cut(s) 51, 548
Hpy188III TCNNGA 2 cut(s) 26, 32
Hpy8I GTNNAC 6 cut(s) 205, 324, 370, 634, 675, 758
HpyAV CCTTC 2 cut(s) 433, 440
HpyCH4III ACNGT 3 cut(s) 631, 717, 730
HpyCH4IV ACGT 3 cut(s) 93, 270, 776
HpyF10VI GCNNNNNNNGC 3 cut(s) 13, 149, 440
HpyF3I CTNAG 3 cut(s) 9, 54, 105
HpySE526I ACGT 3 cut(s) 93, 270, 776
Hsp92II CATG 4 cut(s) 268, 344, 365, 706
Kzo9I GATC 5 cut(s) 28, 41, 160, 183, 618
LweI GCATC 1 cut(s) 539
MaeI CTAG 3 cut(s) 32, 39, 518
MaeII ACGT 3 cut(s) 93, 270, 776
MaeIII GTNAC 5 cut(s) 271, 527, 711, 717, 772
MalI GATC 5 cut(s) 30, 43, 162, 185, 620
MboI GATC 5 cut(s) 28, 41, 160, 183, 618
MboII GAAGA 6 cut(s) 91, 205, 535, 634, 751, 754
MfeI CAATTG 1 cut(s) 766
MhlI GDGCHC 1 cut(s) 677
MluCI AATT 6 cut(s) 121, 241, 317, 387, 398, 766
MlyI GAGTC 2 cut(s) 46, 739
MnlI CCTC 3 cut(s) 142, 191, 253
MslI CAYNNNNRTG 1 cut(s) 434
MspI CCGG 4 cut(s) 158, 169, 486, 752
MspR9I CCNGG 3 cut(s) 503, 709, 753
MunI CAATTG 1 cut(s) 766
Mva1269I GAATGC 1 cut(s) 617
MvaI CCWGG 2 cut(s) 503, 709
MwoI GCNNNNNNNGC 3 cut(s) 13, 149, 440
NciI CCSGG 1 cut(s) 753
NdeII GATC 5 cut(s) 28, 41, 160, 183, 618
NlaIII CATG 4 cut(s) 268, 344, 365, 706
NmuCI GTSAC 2 cut(s) 527, 772
NspV TTCGAA 1 cut(s) 737
PcsI WCGNNNNNNNCGW 1 cut(s) 734
PctI GAATGC 1 cut(s) 617
PfeI GAWTC 2 cut(s) 66, 416
PflMI CCANNNNNTGG 1 cut(s) 508
PkrI GCNGC 2 cut(s) 15, 596
PleI GAGTC 2 cut(s) 46, 739
PpsI GAGTC 2 cut(s) 46, 739
PsiI TTATAA 1 cut(s) 354
Psp6I CCWGG 2 cut(s) 501, 707
PspEI GGTNACC 1 cut(s) 527
PspGI CCWGG 2 cut(s) 501, 707
PspPI GGNCC 1 cut(s) 488
PstI CTGCAG 1 cut(s) 661
PstNI CAGNNNCTG 1 cut(s) 721
RsaI GTAC 2 cut(s) 156, 304
RsaNI GTAC 2 cut(s) 155, 303
RseI CAYNNNNRTG 1 cut(s) 434
SalI GTCGAC 1 cut(s) 632
SatI GCNGC 2 cut(s) 14, 595
Sau3AI GATC 5 cut(s) 28, 41, 160, 183, 618
Sau96I GGNCC 1 cut(s) 488
SchI GAGTC 2 cut(s) 46, 739
ScrFI CCNGG 3 cut(s) 503, 709, 753
SduI GDGCHC 1 cut(s) 677
SetI ASST 8 cut(s) 15, 40, 96, 273, 436, 541, 699, 779
SfaNI GCATC 1 cut(s) 539
SfcI CTRYAG 2 cut(s) 649, 657
SfuI TTCGAA 1 cut(s) 737
SinI GGWCC 1 cut(s) 488
SmiMI CAYNNNNRTG 1 cut(s) 434
Sse9I AATT 6 cut(s) 121, 241, 317, 387, 398, 766
SsiI CCGC 1 cut(s) 595
SspMI CTAG 3 cut(s) 32, 39, 518
StyD4I CCNGG 3 cut(s) 501, 707, 751
TaaI ACNGT 3 cut(s) 631, 717, 730
TaiI ACGT 3 cut(s) 96, 273, 779
TaqI TCGA 2 cut(s) 633, 737
TasI AATT 6 cut(s) 121, 241, 317, 387, 398, 766
TauI GCSGC 1 cut(s) 597
TfiI GAWTC 2 cut(s) 66, 416
TscAI CASTG 4 cut(s) 513, 628, 634, 661
TseFI GTSAC 2 cut(s) 527, 772
TseI GCWGC 1 cut(s) 13
Tsp45I GTSAC 2 cut(s) 527, 772
TspDTI ATGAA 3 cut(s) 380, 442, 628
TspRI CASTG 4 cut(s) 513, 628, 634, 661
Van91I CCANNNNNTGG 1 cut(s) 508
VneI GTGCAC 1 cut(s) 673
VpaK11BI GGWCC 1 cut(s) 488
XapI RAATTY 1 cut(s) 121
XbaI TCTAGA 1 cut(s) 31
XmiI GTMKAC 1 cut(s) 633
XspI CTAG 3 cut(s) 32, 39, 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.