Rh1AG311500

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0001935
Physical Location & Seq
Forward (+)
54495637 .. 54495909
273 bp
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UTR
Exon/CDS
Intron
Rh1AG311500.1

Sequence Viewer

Length: 273 bp
ATGCAATCGACCCTAGGTAGTGTAGTAACTCCCAAGGGTTGGATTGAATGGAATGGAAACCGTGGCATTGACACTCTGTATTATGGTGAGTTCATGAATAAAGGGCCAGGTTCCGAGCTCGCAGGGCGGGTCAGATGGCTCGGTCATCATACTGATCCATCTGAGGCCGATCAGTTTACGGCCACCAACTTTATTGACGGAGATTTTTGGCTGCCATCCACAGGTATCCCATACACATCAGAATTGGGGAGTCCAATACCTCAACCTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
PMEI Pectinesterase

Protein Analysis

90

Amino Acids

9.89

Weight (kDa)

4.63

Isoelectric Point (pI)

36.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 1 - 68 4.4e-23 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 39
AciI CCGC 1 cut(s) 127
AclWI GGATC 1 cut(s) 149
AcoI YGGCCR 1 cut(s) 180
AfiI CCNNNNNNNGG 2 cut(s) 39, 221
AgsI TTSAA 1 cut(s) 47
AjnI CCWGG 1 cut(s) 106
AluBI AGCT 2 cut(s) 118, 270
AluI AGCT 2 cut(s) 118, 270
Alw21I GWGCWC 1 cut(s) 120
AlwI GGATC 1 cut(s) 149
AoxI GGCC 3 cut(s) 104, 165, 180
ApeKI GCWGC 1 cut(s) 211
AspA2I CCTAGG 1 cut(s) 13
AspS9I GGNCC 1 cut(s) 104
AsuHPI GGTGA 1 cut(s) 98
AvrII CCTAGG 1 cut(s) 13
BanII GRGCYC 1 cut(s) 120
Bbv12I GWGCWC 1 cut(s) 120
BbvI GCAGC 1 cut(s) 198
BccI CCATC 3 cut(s) 129, 166, 223
BceAI ACGGC 1 cut(s) 195
BciT130I CCWGG 1 cut(s) 108
BciVI GTATCC 1 cut(s) 236
BfaI CTAG 2 cut(s) 14, 267
BfuI GTATCC 1 cut(s) 236
BisI GCNGC 1 cut(s) 212
BlnI CCTAGG 1 cut(s) 13
BlsI GCNGC 1 cut(s) 213
Bme1390I CCNGG 1 cut(s) 108
BmgT120I GGNCC 1 cut(s) 104
BmiI GGNNCC 1 cut(s) 112
BmrFI CCNGG 1 cut(s) 108
BsaJI CCNNGG 3 cut(s) 13, 33, 61
Bsc4I CCNNNNNNNGG 2 cut(s) 39, 221
BseBI CCWGG 1 cut(s) 108
BseDI CCNNGG 3 cut(s) 13, 33, 61
BseGI GGATG 1 cut(s) 215
BseLI CCNNNNNNNGG 2 cut(s) 39, 221
BseMII CTCAG 1 cut(s) 153
BseXI GCAGC 1 cut(s) 198
BshFI GGCC 3 cut(s) 106, 167, 182
BsiHKAI GWGCWC 1 cut(s) 120
BslI CCNNNNNNNGG 2 cut(s) 39, 221
BsnI GGCC 3 cut(s) 106, 167, 182
Bsp1286I GDGCHC 1 cut(s) 120
Bsp143I GATC 2 cut(s) 154, 169
BspACI CCGC 1 cut(s) 127
BspANI GGCC 3 cut(s) 106, 167, 182
BspCNI CTCAG 1 cut(s) 154
BspHI TCATGA 1 cut(s) 93
BspLI GGNNCC 1 cut(s) 112
BspPI GGATC 1 cut(s) 149
BssECI CCNNGG 3 cut(s) 13, 33, 61
BssMI GATC 2 cut(s) 154, 169
BssT1I CCWWGG 2 cut(s) 13, 33
Bst2UI CCWGG 1 cut(s) 108
Bst4CI ACNGT 1 cut(s) 62
BstC8I GCNNGC 1 cut(s) 120
BstDEI CTNAG 1 cut(s) 162
BstDSI CCRYGG 1 cut(s) 61
BstF5I GGATG 1 cut(s) 215
BstKTI GATC 2 cut(s) 157, 172
BstMBI GATC 2 cut(s) 154, 169
BstMWI GCNNNNNNNGC 1 cut(s) 124
BstNI CCWGG 1 cut(s) 108
BstSCI CCNGG 1 cut(s) 106
BstV1I GCAGC 1 cut(s) 198
BsuI GTATCC 1 cut(s) 236
BsuRI GGCC 3 cut(s) 106, 167, 182
BtgI CCRYGG 1 cut(s) 61
BtsCI GGATG 1 cut(s) 215
Cac8I GCNNGC 1 cut(s) 120
CciI TCATGA 1 cut(s) 93
Cfr13I GGNCC 1 cut(s) 104
CviAII CATG 1 cut(s) 94
CviJI RGCY 7 cut(s) 106, 118, 139, 167, 182, 211, 270
CviKI_1 RGCY 7 cut(s) 106, 118, 139, 167, 182, 211, 270
DdeI CTNAG 1 cut(s) 162
DpnI GATC 2 cut(s) 156, 171
DpnII GATC 2 cut(s) 154, 169
EaeI YGGCCR 1 cut(s) 180
Ecl136II GAGCTC 1 cut(s) 118
Eco130I CCWWGG 2 cut(s) 13, 33
Eco24I GRGCYC 1 cut(s) 120
Eco53kI GAGCTC 1 cut(s) 118
EcoICRI GAGCTC 1 cut(s) 118
EcoRII CCWGG 1 cut(s) 106
EcoT14I CCWWGG 2 cut(s) 13, 33
EcoT38I GRGCYC 1 cut(s) 120
ErhI CCWWGG 2 cut(s) 13, 33
FaeI CATG 1 cut(s) 97
FaiI YATR 4 cut(s) 84, 95, 150, 232
FatI CATG 1 cut(s) 93
FauI CCCGC 1 cut(s) 120
Fnu4HI GCNGC 1 cut(s) 212
FokI GGATG 1 cut(s) 202
FriOI GRGCYC 1 cut(s) 120
Fsp4HI GCNGC 1 cut(s) 212
FspBI CTAG 2 cut(s) 14, 267
GluI GCNGC 1 cut(s) 212
HaeIII GGCC 3 cut(s) 106, 167, 182
Hin1II CATG 1 cut(s) 97
HinfI GANTC 1 cut(s) 250
HphI GGTGA 1 cut(s) 98
Hpy166II GTNNAC 1 cut(s) 177
Hpy188I TCNGA 4 cut(s) 115, 134, 163, 241
Hpy188III TCNNGA 1 cut(s) 94
Hpy8I GTNNAC 1 cut(s) 177
HpyCH4III ACNGT 1 cut(s) 62
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 1 cut(s) 124
HpyF3I CTNAG 1 cut(s) 162
Hsp92II CATG 1 cut(s) 97
Kzo9I GATC 2 cut(s) 154, 169
LpnPI CCDG 4 cut(s) 93, 108, 120, 207
Lsp1109I GCAGC 1 cut(s) 198
MaeI CTAG 2 cut(s) 14, 267
MaeIII GTNAC 1 cut(s) 25
MalI GATC 2 cut(s) 156, 171
MboI GATC 2 cut(s) 154, 169
MhlI GDGCHC 1 cut(s) 120
MluCI AATT 1 cut(s) 242
MlyI GAGTC 1 cut(s) 259
MmeI TCCRAC 1 cut(s) 20
MnlI CCTC 2 cut(s) 157, 270
MspR9I CCNGG 1 cut(s) 108
MvaI CCWGG 1 cut(s) 108
MwoI GCNNNNNNNGC 1 cut(s) 124
NdeII GATC 2 cut(s) 154, 169
NlaIII CATG 1 cut(s) 97
NlaIV GGNNCC 1 cut(s) 112
PagI TCATGA 1 cut(s) 93
PflMI CCANNNNNTGG 1 cut(s) 39
PkrI GCNGC 1 cut(s) 213
PleI GAGTC 1 cut(s) 258
PpsI GAGTC 1 cut(s) 258
Psp124BI GAGCTC 1 cut(s) 120
Psp6I CCWGG 1 cut(s) 106
PspGI CCWGG 1 cut(s) 106
PspN4I GGNNCC 1 cut(s) 112
PspPI GGNCC 1 cut(s) 104
SacI GAGCTC 1 cut(s) 120
SatI GCNGC 1 cut(s) 212
Sau3AI GATC 2 cut(s) 154, 169
Sau96I GGNCC 1 cut(s) 104
SchI GAGTC 1 cut(s) 259
ScrFI CCNGG 1 cut(s) 108
SduI GDGCHC 1 cut(s) 120
SetI ASST 7 cut(s) 19, 112, 120, 226, 262, 268, 272
Sse9I AATT 1 cut(s) 242
SsiI CCGC 1 cut(s) 127
SspMI CTAG 2 cut(s) 14, 267
SstI GAGCTC 1 cut(s) 120
StyD4I CCNGG 1 cut(s) 106
StyI CCWWGG 2 cut(s) 13, 33
TaaI ACNGT 1 cut(s) 62
TaqI TCGA 1 cut(s) 8
TaqII GACCGA 1 cut(s) 131
TasI AATT 1 cut(s) 242
TseI GCWGC 1 cut(s) 211
TspDTI ATGAA 2 cut(s) 82, 110
TspGWI ACGGA 1 cut(s) 213
Van91I CCANNNNNTGG 1 cut(s) 39
XmaJI CCTAGG 1 cut(s) 13
XspI CTAG 2 cut(s) 14, 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.