Rmu_co8224930.1_g000001

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8224930.1
OG0001935
Physical Location & Seq
Forward (+)
55 .. 729
675 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8224930.1_g000001.1.cds

Sequence Viewer

Length: 675 bp
atggacatagggtttgagaacactgccggacaagagaacatgcaggcgttggcggttcgctccagcggcgaccgctccgccttttaccgatgcaagatgaccggaaatcaagacacattgctagtgcaagctggccgccaattcttccgagaatgcaacatcacgggcaccgttgacttcatctttggctatggcagtgctgtctttcaaaaatgcgacatttacatcaagaaaaatccaataggcggaaccagcgtcatcgcggcccatggccgtaacaacccatcagacccatcaggcttttcattccacctctgcaccatcaccggagaccccaatgtggtcattaaagactcgactaacaacggagcgtacctcggtcggaattggggggtttatgcacgaacagtgttcatagattcgaccataagcaatgttctacggccggaaggttggctccagtggcaaggcaagggtgtggacaaagtgtatttcggggagtacatgaacaatggcccgggtgcaaacgctgcaaaccgggtgagctggcctggttgtcatgcgaggatgagccgatctgaggctgatagatttacagtggcaaagcttattgatggaccttcatggctgtcaactgtaggtatcccaaacacaatgggattggatggatcctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
PMEI Pectinesterase

Protein Analysis

224

Amino Acids

24.4

Weight (kDa)

8.27

Isoelectric Point (pI)

29.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 167
AccBSI CCGCTC 1 cut(s) 75
AccII CGCG 1 cut(s) 263
AciI CCGC 7 cut(s) 53, 66, 73, 78, 136, 246, 263
AclWI GGATC 1 cut(s) 663
AcoI YGGCCR 3 cut(s) 133, 271, 443
AfaI GTAC 2 cut(s) 374, 503
AfiI CCNNNNNNNGG 3 cut(s) 245, 340, 580
AgsI TTSAA 1 cut(s) 209
AjnI CCWGG 1 cut(s) 550
AluBI AGCT 3 cut(s) 131, 546, 607
AluI AGCT 3 cut(s) 131, 546, 607
Alw26I GTCTC 1 cut(s) 324
AlwI GGATC 1 cut(s) 663
Ama87I CYCGRG 1 cut(s) 517
AoxI GGCC 6 cut(s) 133, 264, 271, 443, 514, 548
ApeKI GCWGC 1 cut(s) 530
ArsI GACNNNNNNTTYG 3 cut(s) 28, 167, 199
AspS9I GGNCC 3 cut(s) 265, 515, 617
AsuC2I CCSGG 3 cut(s) 518, 519, 539
AsuHPI GGTGA 2 cut(s) 316, 553
AvaI CYCGRG 1 cut(s) 517
AvaII GGWCC 1 cut(s) 617
BaeGI GKGCMC 1 cut(s) 170
BamHI GGATCC 1 cut(s) 668
BanI GGYRCC 1 cut(s) 167
BbvI GCAGC 1 cut(s) 517
BccI CCATC 5 cut(s) 292, 301, 329, 608, 659
BceAI ACGGC 2 cut(s) 258, 458
BciT130I CCWGG 1 cut(s) 552
BciVI GTATCC 1 cut(s) 653
BcnI CCSGG 3 cut(s) 518, 519, 539
BcoDI GTCTC 1 cut(s) 324
BfaI CTAG 1 cut(s) 122
BfmI CTRYAG 1 cut(s) 636
BfuI GTATCC 1 cut(s) 653
BisI GCNGC 4 cut(s) 67, 136, 264, 531
BlsI GCNGC 4 cut(s) 68, 137, 265, 532
Bme1390I CCNGG 4 cut(s) 518, 519, 539, 552
Bme18I GGWCC 1 cut(s) 617
BmeT110I CYCGRG 1 cut(s) 517
BmgT120I GGNCC 3 cut(s) 265, 515, 617
BmiI GGNNCC 4 cut(s) 169, 250, 458, 670
BmrFI CCNGG 4 cut(s) 518, 519, 539, 552
BmsI GCATC 1 cut(s) 80
BplI GAGNNNNNCTC 2 cut(s) 360, 392
BpmI CTGGAG 2 cut(s) 46, 443
BpuMI CCSGG 3 cut(s) 518, 519, 539
BsaI GGTCTC 1 cut(s) 324
BsaJI CCNNGG 3 cut(s) 268, 376, 517
BsaWI WCCGGW 2 cut(s) 101, 326
Bsc4I CCNNNNNNNGG 3 cut(s) 245, 340, 580
Bse1I ACTGG 1 cut(s) 460
Bse3DI GCAATG 2 cut(s) 116, 439
BseBI CCWGG 1 cut(s) 552
BseDI CCNNGG 3 cut(s) 268, 376, 517
BseGI GGATG 2 cut(s) 573, 670
BseLI CCNNNNNNNGG 3 cut(s) 245, 340, 580
BseMI GCAATG 2 cut(s) 116, 439
BseMII CTCAG 1 cut(s) 570
BseNI ACTGG 1 cut(s) 460
BseSI GKGCMC 1 cut(s) 170
BseX3I CGGCCG 1 cut(s) 443
BseXI GCAGC 1 cut(s) 517
BsgI GTGCAG 1 cut(s) 301
Bsh1236I CGCG 1 cut(s) 263
Bsh1285I CGRYCG 3 cut(s) 73, 382, 446
BshFI GGCC 6 cut(s) 135, 266, 273, 445, 516, 550
BshNI GGYRCC 1 cut(s) 167
BsiEI CGRYCG 3 cut(s) 73, 382, 446
BsiHKCI CYCGRG 1 cut(s) 517
BsiSI CCGG 6 cut(s) 27, 102, 327, 446, 518, 538
BslI CCNNNNNNNGG 3 cut(s) 245, 340, 580
BsmAI GTCTC 1 cut(s) 324
BsmI GAATGC 1 cut(s) 158
BsnI GGCC 6 cut(s) 135, 266, 273, 445, 516, 550
Bso31I GGTCTC 1 cut(s) 324
BsoBI CYCGRG 1 cut(s) 517
Bsp1286I GDGCHC 1 cut(s) 170
Bsp143I GATC 2 cut(s) 575, 668
Bsp19I CCATGG 1 cut(s) 268
BspACI CCGC 7 cut(s) 53, 66, 73, 78, 136, 246, 263
BspANI GGCC 6 cut(s) 135, 266, 273, 445, 516, 550
BspCNI CTCAG 1 cut(s) 571
BspFNI CGCG 1 cut(s) 263
BspLI GGNNCC 4 cut(s) 169, 250, 458, 670
BspPI GGATC 1 cut(s) 663
BspT107I GGYRCC 1 cut(s) 167
BspTNI GGTCTC 1 cut(s) 324
BsrBI CCGCTC 1 cut(s) 75
BsrDI GCAATG 2 cut(s) 116, 439
BsrI ACTGG 1 cut(s) 460
BssECI CCNNGG 3 cut(s) 268, 376, 517
BssMI GATC 2 cut(s) 575, 668
BssT1I CCWWGG 1 cut(s) 268
Bst2UI CCWGG 1 cut(s) 552
Bst4CI ACNGT 4 cut(s) 172, 409, 598, 637
BstAPI GCANNNNNTGC 1 cut(s) 530
BstC8I GCNNGC 4 cut(s) 45, 129, 133, 548
BstDEI CTNAG 1 cut(s) 579
BstDSI CCRYGG 1 cut(s) 268
BstF5I GGATG 2 cut(s) 573, 670
BstFNI CGCG 1 cut(s) 263
BstKTI GATC 2 cut(s) 578, 671
BstMAI GTCTC 1 cut(s) 324
BstMBI GATC 2 cut(s) 575, 668
BstMCI CGRYCG 3 cut(s) 73, 382, 446
BstMWI GCNNNNNNNGC 5 cut(s) 66, 72, 252, 463, 530
BstNI CCWGG 1 cut(s) 552
BstNSI RCATGY 1 cut(s) 43
BstSCI CCNGG 4 cut(s) 516, 517, 537, 550
BstSFI CTRYAG 1 cut(s) 636
BstSLI GKGCMC 1 cut(s) 170
BstUI CGCG 1 cut(s) 263
BstV1I GCAGC 1 cut(s) 517
BstX2I RGATCY 1 cut(s) 668
BstYI RGATCY 1 cut(s) 668
BstZI CGGCCG 1 cut(s) 443
BsuI GTATCC 1 cut(s) 653
BsuRI GGCC 6 cut(s) 135, 266, 273, 445, 516, 550
BtgI CCRYGG 1 cut(s) 268
BtgZI GCGATG 1 cut(s) 244
BtsCI GGATG 2 cut(s) 573, 670
BtsI GCAGTG 2 cut(s) 21, 202
BtsIMutI CAGTG 5 cut(s) 21, 202, 414, 467, 603
Cac8I GCNNGC 4 cut(s) 45, 129, 133, 548
Cfr13I GGNCC 3 cut(s) 265, 515, 617
Cfr9I CCCGGG 1 cut(s) 517
CseI GACGC 1 cut(s) 244
Csp6I GTAC 2 cut(s) 373, 502
CviAII CATG 5 cut(s) 40, 269, 505, 560, 624
CviQI GTAC 2 cut(s) 373, 502
DdeI CTNAG 1 cut(s) 579
DpnI GATC 2 cut(s) 577, 670
DpnII GATC 2 cut(s) 575, 668
EaeI YGGCCR 3 cut(s) 133, 271, 443
EagI CGGCCG 1 cut(s) 443
EciI GGCGGA 2 cut(s) 67, 261
EclXI CGGCCG 1 cut(s) 443
Eco130I CCWWGG 1 cut(s) 268
Eco31I GGTCTC 1 cut(s) 324
Eco47I GGWCC 1 cut(s) 617
Eco52I CGGCCG 1 cut(s) 443
Eco88I CYCGRG 1 cut(s) 517
EcoRII CCWGG 1 cut(s) 550
EcoT14I CCWWGG 1 cut(s) 268
ErhI CCWWGG 1 cut(s) 268
FaeI CATG 5 cut(s) 43, 272, 508, 563, 627
FatI CATG 5 cut(s) 39, 268, 504, 559, 623
Fnu4HI GCNGC 4 cut(s) 67, 136, 264, 531
FokI GGATG 1 cut(s) 580
Fsp4HI GCNGC 4 cut(s) 67, 136, 264, 531
FspBI CTAG 1 cut(s) 122
GluI GCNGC 4 cut(s) 67, 136, 264, 531
GsuI CTGGAG 2 cut(s) 46, 443
HaeIII GGCC 6 cut(s) 135, 266, 273, 445, 516, 550
HapII CCGG 6 cut(s) 27, 102, 327, 446, 518, 538
HgaI GACGC 1 cut(s) 244
Hin1II CATG 5 cut(s) 43, 272, 508, 563, 627
HincII GTYRAC 2 cut(s) 175, 633
HindII GTYRAC 2 cut(s) 175, 633
HindIII AAGCTT 1 cut(s) 605
HinfI GANTC 2 cut(s) 353, 419
HpaII CCGG 6 cut(s) 27, 102, 327, 446, 518, 538
HphI GGTGA 2 cut(s) 316, 553
Hpy166II GTNNAC 3 cut(s) 175, 481, 633
Hpy188I TCNGA 4 cut(s) 149, 289, 384, 580
Hpy188III TCNNGA 2 cut(s) 110, 229
Hpy8I GTNNAC 3 cut(s) 175, 481, 633
HpyAV CCTTC 2 cut(s) 443, 630
HpyCH4III ACNGT 4 cut(s) 172, 409, 598, 637
HpyCH4V TGCA 8 cut(s) 43, 93, 127, 156, 318, 401, 524, 533
HpyF10VI GCNNNNNNNGC 5 cut(s) 66, 72, 252, 463, 530
HpyF3I CTNAG 1 cut(s) 579
Hsp92II CATG 5 cut(s) 43, 272, 508, 563, 627
Kzo9I GATC 2 cut(s) 575, 668
LmnI GCTCC 4 cut(s) 65, 80, 368, 462
Lsp1109I GCAGC 1 cut(s) 517
LweI GCATC 1 cut(s) 80
MaeI CTAG 1 cut(s) 122
MaeIII GTNAC 1 cut(s) 275
MalI GATC 2 cut(s) 577, 670
MbiI CCGCTC 1 cut(s) 75
MboI GATC 2 cut(s) 575, 668
MboII GAAGA 1 cut(s) 136
MflI RGATCY 1 cut(s) 668
MhlI GDGCHC 1 cut(s) 170
MluCI AATT 2 cut(s) 140, 385
MlyI GAGTC 1 cut(s) 347
MmeI TCCRAC 1 cut(s) 362
MnlI CCTC 4 cut(s) 323, 386, 558, 574
MseI TTAA 1 cut(s) 348
MspA1I CMGCKG 1 cut(s) 66
MspI CCGG 6 cut(s) 27, 102, 327, 446, 518, 538
MspR9I CCNGG 4 cut(s) 518, 519, 539, 552
Mva1269I GAATGC 1 cut(s) 158
MvaI CCWGG 1 cut(s) 552
MvnI CGCG 1 cut(s) 263
MwoI GCNNNNNNNGC 5 cut(s) 66, 72, 252, 463, 530
NciI CCSGG 3 cut(s) 518, 519, 539
NcoI CCATGG 1 cut(s) 268
NdeII GATC 2 cut(s) 575, 668
NlaIII CATG 5 cut(s) 43, 272, 508, 563, 627
NlaIV GGNNCC 4 cut(s) 169, 250, 458, 670
NspI RCATGY 1 cut(s) 43
PctI GAATGC 1 cut(s) 158
PfeI GAWTC 1 cut(s) 419
PkrI GCNGC 4 cut(s) 68, 137, 265, 532
PleI GAGTC 1 cut(s) 347
PpsI GAGTC 1 cut(s) 347
Psp6I CCWGG 1 cut(s) 550
PspGI CCWGG 1 cut(s) 550
PspN4I GGNNCC 4 cut(s) 169, 250, 458, 670
PspPI GGNCC 3 cut(s) 265, 515, 617
PsuI RGATCY 1 cut(s) 668
RsaI GTAC 2 cut(s) 374, 503
RsaNI GTAC 2 cut(s) 373, 502
SaqAI TTAA 1 cut(s) 348
SatI GCNGC 4 cut(s) 67, 136, 264, 531
Sau3AI GATC 2 cut(s) 575, 668
Sau96I GGNCC 3 cut(s) 265, 515, 617
SchI GAGTC 1 cut(s) 347
ScrFI CCNGG 4 cut(s) 518, 519, 539, 552
SduI GDGCHC 1 cut(s) 170
SetI ASST 8 cut(s) 133, 315, 378, 454, 548, 609, 622, 643
SfaNI GCATC 1 cut(s) 80
SfcI CTRYAG 1 cut(s) 636
SinI GGWCC 1 cut(s) 617
SmaI CCCGGG 1 cut(s) 519
Sse9I AATT 2 cut(s) 140, 385
SsiI CCGC 7 cut(s) 53, 66, 73, 78, 136, 246, 263
SspMI CTAG 1 cut(s) 122
StyD4I CCNGG 4 cut(s) 516, 517, 537, 550
StyI CCWWGG 1 cut(s) 268
TaaI ACNGT 4 cut(s) 172, 409, 598, 637
TaqI TCGA 2 cut(s) 356, 422
TaqII GACCGA 1 cut(s) 368
TasI AATT 2 cut(s) 140, 385
TatI WGTACW 1 cut(s) 501
TauI GCSGC 3 cut(s) 69, 138, 266
TfiI GAWTC 1 cut(s) 419
Tru1I TTAA 1 cut(s) 348
Tru9I TTAA 1 cut(s) 348
TscAI CASTG 5 cut(s) 28, 202, 414, 467, 603
TseI GCWGC 1 cut(s) 530
TspDTI ATGAA 5 cut(s) 169, 294, 403, 521, 612
TspGWI ACGGA 1 cut(s) 381
TspMI CCCGGG 1 cut(s) 517
TspRI CASTG 5 cut(s) 28, 202, 414, 467, 603
VpaK11BI GGWCC 1 cut(s) 617
XceI RCATGY 1 cut(s) 43
XmaI CCCGGG 1 cut(s) 517
XspI CTAG 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.