Rorug02G0629500

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001935
Physical Location & Seq
Forward (+)
74425927 .. 74428814
2888 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0629500.1

Sequence Viewer

Length: 801 bp
ATGTCGCTGGACGGAGTCCAGCCGTACCGAGACACCTTCCACTCGCTCATTTTGGGGTCCATGAAAGGGGCTCGGTTGCAGGACGCGTTCTACTTCTCGGAGCAGATGAAATCCATGGGTTTGGTTCCTGATGGAATGAATGTTGAAATTAACAATGCGATCTGCATCTGCTGGTATGCAATTGTCCGCGACATGACTGCTGCTGGTCTTGGGTTAAATAAGTTTTGCTATGCGGGACTTATAACTGCACTTAGGAACAAGACACCCTTACCAGATGATTATGCTGCCAAAATTATAGAGTTTGTGGAGCGGTCAAAAGAGTGGTCATCGGTTGAAGCTTCAAGTGAAACCGCTCAAAATGTAATGACGGGGGTAACAAAAGAGGAGTTGTATAACATGCCAACAGCTGAGTATGTTAATAGGCGTGGATTTTTTAATAGGCCGCTAACTGTGTATCATGTTGCATTTCATGCTTGTGCGGACCTACGAAATGTTGAGGTGATGGAAGCTCTTTTGGACATGCTAAAAAAGGATGGGAAATTTCCAGATACCTATATTGTGATTCAAACTATGAGGGATATTAATTGTGGTGTGCAAACACTTGAAGAATATCTGAAGTCAGGGAGGCCTCCTGTACCAGAACTCTATAGTATACTTGCGGAGGGCGCCATGATTGGATATACACCCAAGGGAATGCAAATCGCCCAAAAGGCATTGGAAGATATGAATTCCAGGAACTTCTACTTGCTCCCAAGACAAGGGACTGATCTTCTCCTTGCAGCTGCCGGTGAAAAGCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
PMEI Pectinesterase

Protein Analysis

266

Amino Acids

29.8

Weight (kDa)

5.37

Isoelectric Point (pI)

41.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 242
AccB1I GGYRCC 1 cut(s) 665
AccBSI CCGCTC 2 cut(s) 310, 353
AccI GTMKAC 1 cut(s) 652
AccII CGCG 2 cut(s) 86, 189
AciI CCGC 7 cut(s) 187, 233, 310, 351, 443, 479, 659
AcsI RAATTY 2 cut(s) 539, 727
AcuI CTGAAG 1 cut(s) 635
AcyI GRCGYC 1 cut(s) 666
AfaI GTAC 2 cut(s) 26, 636
AfiI CCNNNNNNNGG 2 cut(s) 66, 758
AflIII ACRYGT 1 cut(s) 84
AgsI TTSAA 5 cut(s) 146, 335, 342, 566, 605
AjnI CCWGG 1 cut(s) 731
AluBI AGCT 4 cut(s) 338, 407, 509, 782
AluI AGCT 4 cut(s) 338, 407, 509, 782
Alw26I GTCTC 1 cut(s) 24
AoxI GGCC 2 cut(s) 440, 626
ApeKI GCWGC 4 cut(s) 200, 284, 779, 782
ApoI RAATTY 2 cut(s) 539, 727
ArsI GACNNNNNNTTYG 2 cut(s) 308, 340
AseI ATTAAT 1 cut(s) 582
AspLEI GCGC 1 cut(s) 668
AspS9I GGNCC 2 cut(s) 57, 481
AsuHPI GGTGA 2 cut(s) 511, 800
AvaII GGWCC 2 cut(s) 57, 481
BanI GGYRCC 1 cut(s) 665
BanII GRGCYC 1 cut(s) 73
BbvI GCAGC 4 cut(s) 187, 271, 769, 791
BccI CCATC 3 cut(s) 125, 496, 527
BceAI ACGGC 1 cut(s) 7
BcgI CGANNNNNNTGC 2 cut(s) 179, 213
BciT130I CCWGG 1 cut(s) 733
BcoDI GTCTC 1 cut(s) 24
BfmI CTRYAG 1 cut(s) 646
BfoI RGCGCY 1 cut(s) 669
BglI GCCNNNNNGGC 1 cut(s) 710
BisI GCNGC 5 cut(s) 201, 285, 443, 780, 783
BlsI GCNGC 5 cut(s) 202, 286, 444, 781, 784
Bme1390I CCNGG 1 cut(s) 733
Bme18I GGWCC 2 cut(s) 57, 481
BmgT120I GGNCC 2 cut(s) 57, 481
BmiI GGNNCC 3 cut(s) 58, 126, 667
BmrFI CCNGG 1 cut(s) 733
BmsI GCATC 1 cut(s) 174
BsaBI GATNNNNATC 1 cut(s) 164
BsaHI GRCGYC 1 cut(s) 666
BsaJI CCNNGG 2 cut(s) 114, 687
Bsc4I CCNNNNNNNGG 2 cut(s) 66, 758
Bse118I RCCGGY 1 cut(s) 785
Bse8I GATNNNNATC 1 cut(s) 164
BseBI CCWGG 1 cut(s) 733
BseDI CCNNGG 2 cut(s) 114, 687
BseGI GGATG 1 cut(s) 538
BseJI GATNNNNATC 1 cut(s) 164
BseLI CCNNNNNNNGG 2 cut(s) 66, 758
BseMII CTCAG 1 cut(s) 399
BseRI GAGGAG 1 cut(s) 398
BseXI GCAGC 4 cut(s) 187, 271, 769, 791
BsgI GTGCAG 1 cut(s) 231
Bsh1236I CGCG 2 cut(s) 86, 189
BshFI GGCC 2 cut(s) 442, 628
BshNI GGYRCC 1 cut(s) 665
BsiSI CCGG 1 cut(s) 786
BslFI GGGAC 2 cut(s) 249, 775
BslI CCNNNNNNNGG 2 cut(s) 66, 758
BsmAI GTCTC 1 cut(s) 24
BsmFI GGGAC 2 cut(s) 249, 775
BsmI GAATGC 1 cut(s) 699
BsnI GGCC 2 cut(s) 442, 628
Bsp1286I GDGCHC 1 cut(s) 73
Bsp143I GATC 2 cut(s) 159, 766
Bsp19I CCATGG 1 cut(s) 114
BspACI CCGC 7 cut(s) 187, 233, 310, 351, 443, 479, 659
BspANI GGCC 2 cut(s) 442, 628
BspCNI CTCAG 1 cut(s) 400
BspFNI CGCG 2 cut(s) 86, 189
BspLI GGNNCC 3 cut(s) 58, 126, 667
BspT107I GGYRCC 1 cut(s) 665
BsrBI CCGCTC 2 cut(s) 310, 353
BsrFI RCCGGY 1 cut(s) 785
BssAI RCCGGY 1 cut(s) 785
BssECI CCNNGG 2 cut(s) 114, 687
BssMI GATC 2 cut(s) 159, 766
BssNAI GTATAC 1 cut(s) 653
BssNI GRCGYC 1 cut(s) 666
BssT1I CCWWGG 2 cut(s) 114, 687
Bst1107I GTATAC 1 cut(s) 653
Bst2UI CCWGG 1 cut(s) 733
Bst4CI ACNGT 1 cut(s) 451
BstACI GRCGYC 1 cut(s) 666
BstAPI GCANNNNNTGC 1 cut(s) 470
BstDEI CTNAG 2 cut(s) 251, 408
BstDSI CCRYGG 1 cut(s) 114
BstF5I GGATG 1 cut(s) 538
BstFNI CGCG 2 cut(s) 86, 189
BstH2I RGCGCY 1 cut(s) 669
BstHHI GCGC 1 cut(s) 668
BstKTI GATC 2 cut(s) 162, 769
BstMAI GTCTC 1 cut(s) 24
BstMBI GATC 2 cut(s) 159, 766
BstMWI GCNNNNNNNGC 3 cut(s) 470, 665, 710
BstNI CCWGG 1 cut(s) 733
BstNSI RCATGY 2 cut(s) 400, 523
BstSCI CCNGG 1 cut(s) 731
BstSFI CTRYAG 1 cut(s) 646
BstUI CGCG 2 cut(s) 86, 189
BstV1I GCAGC 4 cut(s) 187, 271, 769, 791
BstXI CCANNNNNNTGG 1 cut(s) 121
BstZ17I GTATAC 1 cut(s) 653
BsuRI GGCC 2 cut(s) 442, 628
BtgI CCRYGG 1 cut(s) 114
BtsCI GGATG 1 cut(s) 538
CfoI GCGC 1 cut(s) 668
Cfr10I RCCGGY 1 cut(s) 785
Cfr13I GGNCC 2 cut(s) 57, 481
CseI GACGC 1 cut(s) 92
Csp6I GTAC 2 cut(s) 25, 635
CviAII CATG 8 cut(s) 61, 115, 193, 397, 458, 470, 520, 670
CviJI RGCY 9 cut(s) 22, 71, 338, 407, 442, 509, 628, 782, 796
CviKI_1 RGCY 9 cut(s) 22, 71, 338, 407, 442, 509, 628, 782, 796
CviQI GTAC 2 cut(s) 25, 635
DdeI CTNAG 2 cut(s) 251, 408
DinI GGCGCC 1 cut(s) 667
DpnI GATC 2 cut(s) 161, 768
DpnII GATC 2 cut(s) 159, 766
Eco130I CCWWGG 2 cut(s) 114, 687
Eco147I AGGCCT 1 cut(s) 628
Eco24I GRGCYC 1 cut(s) 73
Eco47I GGWCC 2 cut(s) 57, 481
Eco57I CTGAAG 1 cut(s) 635
EcoRI GAATTC 1 cut(s) 727
EcoRII CCWGG 1 cut(s) 731
EcoT14I CCWWGG 2 cut(s) 114, 687
EcoT38I GRGCYC 1 cut(s) 73
EgeI GGCGCC 1 cut(s) 667
EheI GGCGCC 1 cut(s) 667
ErhI CCWWGG 2 cut(s) 114, 687
FaeI CATG 8 cut(s) 64, 118, 196, 400, 461, 473, 523, 673
FalI AAGNNNNNCTT 2 cut(s) 251, 283
FaqI GGGAC 2 cut(s) 249, 775
FatI CATG 8 cut(s) 60, 114, 192, 396, 457, 469, 519, 669
FauI CCCGC 1 cut(s) 226
FblI GTMKAC 1 cut(s) 652
Fnu4HI GCNGC 5 cut(s) 201, 285, 443, 780, 783
FokI GGATG 1 cut(s) 545
FriOI GRGCYC 1 cut(s) 73
Fsp4HI GCNGC 5 cut(s) 201, 285, 443, 780, 783
GlaI GCGC 1 cut(s) 667
GluI GCNGC 5 cut(s) 201, 285, 443, 780, 783
HaeII RGCGCY 1 cut(s) 669
HaeIII GGCC 2 cut(s) 442, 628
HapII CCGG 1 cut(s) 786
HgaI GACGC 1 cut(s) 92
HhaI GCGC 1 cut(s) 668
Hin1I GRCGYC 1 cut(s) 666
Hin1II CATG 8 cut(s) 64, 118, 196, 400, 461, 473, 523, 673
Hin6I GCGC 1 cut(s) 666
HinP1I GCGC 1 cut(s) 666
HindIII AAGCTT 1 cut(s) 336
HinfI GANTC 2 cut(s) 15, 562
HpaII CCGG 1 cut(s) 786
HphI GGTGA 2 cut(s) 511, 800
Hpy166II GTNNAC 1 cut(s) 653
Hpy188I TCNGA 2 cut(s) 100, 615
Hpy188III TCNNGA 2 cut(s) 128, 545
Hpy8I GTNNAC 1 cut(s) 653
HpyAV CCTTC 1 cut(s) 46
HpyCH4III ACNGT 1 cut(s) 451
HpyCH4V TGCA 8 cut(s) 79, 165, 179, 248, 464, 595, 697, 779
HpyF10VI GCNNNNNNNGC 3 cut(s) 470, 665, 710
HpyF3I CTNAG 2 cut(s) 251, 408
Hsp92I GRCGYC 1 cut(s) 666
Hsp92II CATG 8 cut(s) 64, 118, 196, 400, 461, 473, 523, 673
HspAI GCGC 1 cut(s) 666
KasI GGCGCC 1 cut(s) 665
Kzo9I GATC 2 cut(s) 159, 766
LmnI GCTCC 3 cut(s) 100, 307, 753
Lsp1109I GCAGC 4 cut(s) 187, 271, 769, 791
LweI GCATC 1 cut(s) 174
MaeIII GTNAC 1 cut(s) 373
MalI GATC 2 cut(s) 161, 768
MbiI CCGCTC 2 cut(s) 310, 353
MboI GATC 2 cut(s) 159, 766
MboII GAAGA 3 cut(s) 617, 731, 761
MfeI CAATTG 1 cut(s) 180
MhlI GDGCHC 1 cut(s) 73
MluCI AATT 6 cut(s) 147, 180, 291, 539, 583, 727
MluI ACGCGT 1 cut(s) 84
Mly113I GGCGCC 1 cut(s) 666
MlyI GAGTC 1 cut(s) 24
MnlI CCTC 6 cut(s) 376, 490, 567, 618, 639, 655
MseI TTAA 5 cut(s) 150, 215, 417, 435, 582
MslI CAYNNNNRTG 1 cut(s) 474
MspA1I CMGCKG 2 cut(s) 407, 782
MspI CCGG 1 cut(s) 786
MspR9I CCNGG 1 cut(s) 733
MunI CAATTG 1 cut(s) 180
Mva1269I GAATGC 1 cut(s) 699
MvaI CCWGG 1 cut(s) 733
MvnI CGCG 2 cut(s) 86, 189
MwoI GCNNNNNNNGC 3 cut(s) 470, 665, 710
NarI GGCGCC 1 cut(s) 666
NcoI CCATGG 1 cut(s) 114
NdeII GATC 2 cut(s) 159, 766
NlaIII CATG 8 cut(s) 64, 118, 196, 400, 461, 473, 523, 673
NlaIV GGNNCC 3 cut(s) 58, 126, 667
NspI RCATGY 2 cut(s) 400, 523
PceI AGGCCT 1 cut(s) 628
PctI GAATGC 1 cut(s) 699
PfeI GAWTC 1 cut(s) 562
PflFI GACNNNGTC 1 cut(s) 14
PfoI TCCNGGA 1 cut(s) 731
PkrI GCNGC 5 cut(s) 202, 286, 444, 781, 784
PleI GAGTC 1 cut(s) 23
PluTI GGCGCC 1 cut(s) 669
PpsI GAGTC 1 cut(s) 23
PshBI ATTAAT 1 cut(s) 582
PsiI TTATAA 1 cut(s) 242
Psp6I CCWGG 1 cut(s) 731
PspGI CCWGG 1 cut(s) 731
PspN4I GGNNCC 3 cut(s) 58, 126, 667
PspPI GGNCC 2 cut(s) 57, 481
PsyI GACNNNGTC 1 cut(s) 14
PvuII CAGCTG 2 cut(s) 407, 782
RsaI GTAC 2 cut(s) 26, 636
RsaNI GTAC 2 cut(s) 25, 635
RseI CAYNNNNRTG 1 cut(s) 474
SaqAI TTAA 5 cut(s) 150, 215, 417, 435, 582
SatI GCNGC 5 cut(s) 201, 285, 443, 780, 783
Sau3AI GATC 2 cut(s) 159, 766
Sau96I GGNCC 2 cut(s) 57, 481
SchI GAGTC 1 cut(s) 24
ScrFI CCNGG 1 cut(s) 733
SduI GDGCHC 1 cut(s) 73
SetI ASST 8 cut(s) 38, 340, 409, 486, 501, 511, 554, 784
SfaNI GCATC 1 cut(s) 174
SfcI CTRYAG 1 cut(s) 646
SfoI GGCGCC 1 cut(s) 667
SinI GGWCC 2 cut(s) 57, 481
SmiMI CAYNNNNRTG 1 cut(s) 474
Sse9I AATT 6 cut(s) 147, 180, 291, 539, 583, 727
SseBI AGGCCT 1 cut(s) 628
SsiI CCGC 7 cut(s) 187, 233, 310, 351, 443, 479, 659
SspDI GGCGCC 1 cut(s) 665
StuI AGGCCT 1 cut(s) 628
StyD4I CCNGG 1 cut(s) 731
StyI CCWWGG 2 cut(s) 114, 687
TaaI ACNGT 1 cut(s) 451
TasI AATT 6 cut(s) 147, 180, 291, 539, 583, 727
TauI GCSGC 1 cut(s) 445
TfiI GAWTC 1 cut(s) 562
Tru1I TTAA 5 cut(s) 150, 215, 417, 435, 582
Tru9I TTAA 5 cut(s) 150, 215, 417, 435, 582
TseI GCWGC 4 cut(s) 200, 284, 779, 782
TspDTI ATGAA 5 cut(s) 77, 122, 152, 458, 740
TspGWI ACGGA 1 cut(s) 27
Tth111I GACNNNGTC 1 cut(s) 14
VpaK11BI GGWCC 2 cut(s) 57, 481
VspI ATTAAT 1 cut(s) 582
XapI RAATTY 2 cut(s) 539, 727
XceI RCATGY 2 cut(s) 400, 523
XmiI GTMKAC 1 cut(s) 652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.